BGC0000346

General information about the BGC

MIBiG accession	BGC0000346
Short description	epoxomicin biosynthetic gene cluster from Goodfellowiella coeruleoviolacea
Status	Minimal annotation: no A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s) Completeness: complete Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)	NRP Polyketide (Other)
Loci	NCBI GenBank: KF647219.1
Compounds	epoxomicin
Species	Goodfellowiella coeruleoviolacea [taxonomy]
References	Genetic basis for the biosynthesis of the pharmaceutically important class of epoxyketone proteasome inhibitors. Schorn M et al., ACS Chem Biol (2014) PMID:24168704 Epoxomicin, a new antitumor agent of microbial origin. Hanada M et al., J Antibiot (Tokyo) (1992) PMID:1468981 Total synthesis of the potent proteasome inhibitor epoxomicin: a useful tool for understanding proteasome biology. Sin N et al., Bioorg Med Chem Lett (1999) PMID:10465562 Epoxomicin, a potent and selective proteasome inhibitor, exhibits in vivo antiinflammatory activity. Meng L et al., Proc Natl Acad Sci U S A (1999) PMID:10468620

Chemical products information

epoxomicin

Copy SMILES

C₂₈H₅₀N₄O₇

Chemical database entries
PubCHEM
PubCHEM

List of genes involved in compound(s) production

Identifiers	Position	Product	Extra
AHB38501.1	177 - 1778 (-)	FAD-dependent oxidoreductase	copy AA seq copy Nt seq
AHB38494.1 epxA	1987 - 2652 (+)	TetR-like transcriptional regulator	copy AA seq copy Nt seq
AHB38495.1 epxB	2768 - 4381 (-)	FAD-dependent oxidoreductase	copy AA seq copy Nt seq
AHB38496.1 epxC	4862 - 6139 (+)	cytochrome P450	copy AA seq copy Nt seq
AHB38497.1 epxD	6250 - 21543 (+)	non-ribosomal peptide synthetase	copy AA seq copy Nt seq
AHB38498.1 epxE	21547 - 27549 (+)	polyketide synthase	copy AA seq copy Nt seq
AHB38499.1 epxF	27570 - 29261 (+)	acyl-CoA dehydrogenase	copy AA seq copy Nt seq
AHB38500.1	29429 - 29878 (-)	MarR-like transcriptional regulator	copy AA seq copy Nt seq

Polyketide-specific information

Subclass	Other
Cyclic?	no

Polyketide-synthases

Genes	Properties	Modules
epxE	Synthase subclass: Modular type I	Module 5 Specificity: Malonyl-CoA Evidence for specificity: Sequence-based prediction Genes: epxE Core domains: Ketosynthase, Acyltransferase, Thiolation (ACP/PCP) Scaffold-modifying domains: Methylation

NRP-specific information

NRP-synthases

Gene	Modules
epxD	Module 1 Specificity: isoleucine Evidence for specificity: Sequence-based prediction Condensation domain type: Starter Modification domains: Methylation
	Module 2 Specificity: isoleucine Evidence for specificity: Sequence-based prediction Condensation domain type: LCL
	Module 3 Specificity: threonine Evidence for specificity: Sequence-based prediction Condensation domain type: LCL
	Module 4 Specificity: leucine Evidence for specificity: Condensation domain type: LCL

Annotation changelog

MIBiG version	Submitter	Notes
1.0	Hidden contributor (ID: MQCAQSV425YQGLSERITRLP7K, no GDPR consent given).	Submitted
2.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Migrated from v1.4 Updated compound(s) information (NPAtlas curation)
3.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Corrected NRP module activity Removed ketoreductase stereochemistry annotation from modules without ketoreductases Updated NRP substrate specificities
3.1	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Update chemical activity to schema version 2.11

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters