BGC0000351 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000351 (Mycolicibacterium smegmatis MC2 155)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	24,370	exochelin	NRPS:Type I	100%

BGC0000351 - Region 1 - NRPS

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Location: 1 - 24,370 nt. (total: 24,370 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 24,371
Label		BGC0000351

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000351	1.00	NRP	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0000426	0.45	NRP	sevadicin	Paenibacillus larvae
BGC0001133	0.40	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.36	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002437	0.33	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001657	0.33	NRP	lysocin	Lysobacter sp. RH2180-5
BGC0001132	0.31	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833	0.30	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0000449	0.30	NRP	tridecaptin	Paenibacillus terrae
BGC0002135	0.29	NRP	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000351	1.00	NRP	exochelin	Mycolicibacterium smegmatis MC2 155
BGC0000426	0.75	NRP	sevadicin	Paenibacillus larvae
BGC0001133	0.72	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.69	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002437	0.66	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001657	0.66	NRP	lysocin	Lysobacter sp. RH2180-5
BGC0001132	0.64	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833	0.63	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0000449	0.63	NRP	tridecaptin	Paenibacillus terrae
BGC0002135	0.63	NRP	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 24370:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Orn - bAla) + (D-OH-Orn - D-Thr - OH-Orn - Ser)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

fxbB

Search NORINE for peptide: strict or relaxed

AMP-binding (30..438): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DINYWGGIGK
Nearest Stachelhaus code(s):: Orn DINYWGGIGK (100% 8Å match); Fo-OH-Orn DINYWGGIGK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1546..1928): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IDTLISSGDK
Nearest Stachelhaus code(s):: bAla IDTLISSGDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

fxbC

Search NORINE for peptide: strict or relaxed

AMP-binding (191..472): OH-Orn

nrpys: OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DMENLGLINK
Nearest Stachelhaus code(s):: OH-Orn DMENLGLINK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1579..1976): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3100..3502): OH-Orn

nrpys: OH-Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DMENLGLINK
Nearest Stachelhaus code(s):: OH-Orn DMENLGLINK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4158..4533): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DTWDTGLVDK
Nearest Stachelhaus code(s):: Orn DTEDMGLVDK (53% 8Å match)
Stachelhaus code match:: 80% (moderate)