BGC0000354 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000354 (Actinoplanes friuliensis)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS-like,NRPS	1	70,820	friulimicin A/friulimicin B/friulimicin C/friulimicin D	NRPS:Type I	100%

BGC0000354 - Region 1 - NRPS,NRPS-like

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Location: 1 - 70,820 nt. (total: 70,820 nt) Show pHMM detection rules used

Region on contig edge.

NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))
NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 70,821
Label		BGC0000354

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000354	1.48	NRP	friulimicin A, friulimicin B, friulimicin C, friulimicin D	Actinoplanes friuliensis
BGC0001133	0.73	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.72	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002017	0.64	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0002437	0.64	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0000417	0.63	NRP	rhodochelin	Rhodococcus jostii RHA1
BGC0000457	0.62	NRP	vicibactin	Rhizobium etli CFN 42
BGC0002405	0.61	Saccharide	mid-chain acyl sugars	Solanum lycopersicum
BGC0002135	0.61	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001137	0.60	Alkaloid	marinacarboline A, marinacarboline B, marinacarboline C, marinacarboline D	Marinactinospora thermotolerans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000354	1.00	NRP	friulimicin A, friulimicin B, friulimicin C, friulimicin D	Actinoplanes friuliensis
BGC0002071	0.77	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001133	0.75	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0000331	0.69	NRP	cyanopeptolin	Planktothrix agardhii NIVA-CYA 116
BGC0001844	0.68	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002017	0.68	NRP	potensibactin	Nocardiopsis potens DSM 45234
BGC0000417	0.68	NRP	rhodochelin	Rhodococcus jostii RHA1
BGC0001758	0.68	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001131	0.68	NRP	ambactin	Xenorhabdus miraniensis
BGC0002135	0.68	NRP	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 70820:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val - Pro) + (X - Asp - Gly - Asp - Gly - D-Dab) + (X - D-Hpr) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 0 - 31831:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - Asp - Gly - Asp - Gly - D-Dab) + (X - D-Hpr) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 825 - 70820:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val - Pro) + (X - Asp - Gly - Asp - Gly - D-Dab) + (X - D-Hpr) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

pstA

AMP-binding (212..619): X

nrpys: dnmeProp, Dab

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DIWQSTADDK
Nearest Stachelhaus code(s):: dnmeProp or Dab DIWQSTADDK (100% 8Å match); Asp DIWQSTADDK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

lipA

CAL_domain (42..468): pk

minowa: NH2

Prediction, score:

NH2: 149.0
AHBA: 8.0
Acetyl-CoA: 7.5
shikimic_acid: 0.0
fatty_acid: -3.4

pstB

Search NORINE for peptide: strict or relaxed

AMP-binding (460..836): X

nrpys: Asn, Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGDVNK
Nearest Stachelhaus code(s):: Asn or Asp DLTKVGDVNK (100% 8Å match); OH-Asn DLTKVGDVNK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2047..2455): Hpr

nrpys: Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pip (Hpr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFQFFGVAVK
Nearest Stachelhaus code(s):: Hpr DFQFFGVAVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

pstC

Search NORINE for peptide: strict or relaxed

AMP-binding (466..855): X

nrpys: bMe-Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKMGVVNK
Nearest Stachelhaus code(s):: bMe-Asp DLTKMGVVNK (100% 8Å match); bMe-Asp DLTKMGVVNK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1503..1873): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGAVNK
Nearest Stachelhaus code(s):: Asp DLTKVGAVNK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2523..2902): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLVWK
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3546..3917): Asp

nrpys: Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKVGAVNK
Nearest Stachelhaus code(s):: Asp DLTKVGAVNK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4565..4944): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLVWK
Nearest Stachelhaus code(s):: Gly DILQLGLVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5597..5989): Dab

nrpys: Dab

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFWGEVFK
Nearest Stachelhaus code(s):: Dab DAFFWGEVFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

pstD

Search NORINE for peptide: strict or relaxed

AMP-binding (465..856): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYWWGGTFK
Nearest Stachelhaus code(s):: Val DAYWWGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1519..1924): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQYVAHVVK
Nearest Stachelhaus code(s):: Pro DVQYVAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)