BGC0000364 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000364 (Mycobacteroides abscessus (Mycobacterium abscessus))

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	NRPS	1	40,072	High	glycopeptidolipid	NRPS:Type I

BGC0000364 - Region 1 - NRPS

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Location: 1 - 40,072 nt. (total: 40,072 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 40,073
Label		BGC0000364

Tailoring functions

Transferases: acyltransferase glycosyltransferase methyltransferase transferase

atf1: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

atf2: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

fmt: methyltransferase

smcogs: SMCOG1089: methyltransferase

gtf1: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

gtf2: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

gtf3: glycosyltransferase

smcogs: SMCOG1102: glycosyltransferase

rmlA: glucose-1-phosphate adenylyl/thymidylyltransferase

smcogs: SMCOG1064: glucose-1-phosphate adenylyl/thymidylyltransferase

Lyases: dehydratase

rmlB: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: ligase

mps1: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

mps2: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: transport

mmpl4a: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

mmpl4b: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000364.4	0.98	NRPS	glycopeptidolipid	Mycobacteroides abscessus
BGC0000363.4	0.86	NRPS	glycopeptidolipid	Mycobacteroides chelonae
BGC0000426.5	0.66	NRPS	sevadicin	Paenibacillus larvae
BGC0000365.4	0.65	NRPS	glycopeptidolipid	Mycolicibacterium smegmatis MC2 155
BGC0001133.4	0.64	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.63	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	0.62	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.62	NRPS	AbT1	Aureobasidium pullulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000364.4	0.99	NRPS	glycopeptidolipid	Mycobacteroides abscessus
BGC0000363.4	0.87	NRPS	glycopeptidolipid	Mycobacteroides chelonae
BGC0000365.4	0.67	NRPS	glycopeptidolipid	Mycolicibacterium smegmatis MC2 155
BGC0000426.5	0.66	NRPS	sevadicin	Paenibacillus larvae
BGC0001133.4	0.64	NRPS	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001261.3	0.63	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	0.62	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.62	NRPS	AbT1	Aureobasidium pullulans

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 999 - 40072:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Phe - D-Thr - D-Ala) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

mps1: Phe - Thr

Search NORINE for peptide: strict or relaxed

AMP-binding (466..857): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWTAVAMCK
Nearest Stachelhaus code(s):: Phe DVWTAVAMCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1973..2374): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DYWSVGMVHK
Nearest Stachelhaus code(s):: Thr DYWSVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

mps2: Ala - X

Search NORINE for peptide: strict or relaxed

AMP-binding (46..434): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWCIAAIYK
Nearest Stachelhaus code(s):: Ala DVWCIAAIYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1556..1951): X

nrpys: Aol

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DIWFVAGIYK
Nearest Stachelhaus code(s):: Aol DIWFVAGIYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor