Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000370.1:
Cluster 1NRP116752
The following clusters are from record BGC0000370.2:
Cluster 2NRP13013
The following clusters are from record BGC0000370.3:
Cluster 3NRP17042
The following clusters are from record BGC0000370.4:
Cluster 4NRP11506
The following clusters are from record BGC0000370.5:
Cluster 5NRP11805
The following clusters are from record BGC0000370.6:
Cluster 6NRP11907
The following clusters are from record BGC0000370.7:
Cluster 7NRP11683
The following clusters are from record BGC0000370.8:
Cluster 8NRP17140

BGC0000370, HC-toxin biosynthetic gene cluster from Bipolaris zeicola. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: HC-toxin
PubChem ID: 107864
SMILES string: Copy to clipboard
Molecular formula: C21H32N4O6
Average molecular mass: 436.509 Da
Molecular activity: Inhibitor
Molecular target: histone deacetylase RPD3-class

Class-specific details

Biosynthetic class(es):
NRP
Other (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
HTS1 (AAA33023.2)
Module 1
A specificity: Proline
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: DCL
Scaffold-modifying domain: Other
Module 2
A specificity: Alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 3
A specificity: D-alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 4
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: DCL

Gene cluster description

HC-toxin (BGC0000370). Gene Cluster 1. Biosynthetic class = NRP. GenBank M98024. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

General MIBiG information on this cluster

Complete gene cluster sequence?incomplete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Jonathan Walton (Michigan State University)

Literature references

1. Ahn JH, Walton JD. (1996) Chromosomal organization of TOX2, a complex locus controlling host-selective toxin biosynthesis in Cochliobolus carbonum. Plant Cell 8(5):887-97. doi: 10.1105/tpc.8.5.887.
2. Ahn JH et al. (2002) An extended physical map of the TOX2 locus of Cochliobolus carbonum required for biosynthesis of HC-toxin. Fungal Genet Biol 35(1):31-8. doi: 10.1006/fgbi.2001.1305.
3. Wight WD et al. (2013) Conservation of the genes for HC-toxin biosynthesis in Alternaria jesenskae. BMC Microbiol 13:165. doi: 10.1186/1471-2180-13-165.
4. Walton JD. (2006) HC-toxin. Phytochemistry 67(14):1406-13. doi: 10.1016/j.phytochem.2006.05.033.

BGC0000370, HC-toxin biosynthetic gene cluster from Bipolaris zeicola. Locus 2. Full MIBiG entry.

Chemical compounds

Compound: HC-toxin
PubChem ID: 107864
SMILES string: Copy to clipboard
Molecular formula: C21H32N4O6
Average molecular mass: 436.509 Da
Molecular activity: Inhibitor
Molecular target: histone deacetylase RPD3-class

Class-specific details

Biosynthetic class(es):
NRP
Other (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
HTS1 (AAA33023.2)
Module 1
A specificity: Proline
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: DCL
Scaffold-modifying domain: Other
Module 2
A specificity: Alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 3
A specificity: D-alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 4
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: DCL

Gene cluster description

HC-toxin (BGC0000370). Gene Cluster 2. Biosynthetic class = NRP. GenBank L48797. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

General MIBiG information on this cluster

Complete gene cluster sequence?incomplete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Jonathan Walton (Michigan State University)

Literature references

1. Ahn JH, Walton JD. (1996) Chromosomal organization of TOX2, a complex locus controlling host-selective toxin biosynthesis in Cochliobolus carbonum. Plant Cell 8(5):887-97. doi: 10.1105/tpc.8.5.887.
2. Ahn JH et al. (2002) An extended physical map of the TOX2 locus of Cochliobolus carbonum required for biosynthesis of HC-toxin. Fungal Genet Biol 35(1):31-8. doi: 10.1006/fgbi.2001.1305.
3. Wight WD et al. (2013) Conservation of the genes for HC-toxin biosynthesis in Alternaria jesenskae. BMC Microbiol 13:165. doi: 10.1186/1471-2180-13-165.
4. Walton JD. (2006) HC-toxin. Phytochemistry 67(14):1406-13. doi: 10.1016/j.phytochem.2006.05.033.

BGC0000370, HC-toxin biosynthetic gene cluster from Bipolaris zeicola. Locus 3. Full MIBiG entry.

Chemical compounds

Compound: HC-toxin
PubChem ID: 107864
SMILES string: Copy to clipboard
Molecular formula: C21H32N4O6
Average molecular mass: 436.509 Da
Molecular activity: Inhibitor
Molecular target: histone deacetylase RPD3-class

Class-specific details

Biosynthetic class(es):
NRP
Other (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
HTS1 (AAA33023.2)
Module 1
A specificity: Proline
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: DCL
Scaffold-modifying domain: Other
Module 2
A specificity: Alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 3
A specificity: D-alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 4
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: DCL

Gene cluster description

HC-toxin (BGC0000370). Gene Cluster 3. Biosynthetic class = NRP. GenBank U73650. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

General MIBiG information on this cluster

Complete gene cluster sequence?incomplete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Jonathan Walton (Michigan State University)

Literature references

1. Ahn JH, Walton JD. (1996) Chromosomal organization of TOX2, a complex locus controlling host-selective toxin biosynthesis in Cochliobolus carbonum. Plant Cell 8(5):887-97. doi: 10.1105/tpc.8.5.887.
2. Ahn JH et al. (2002) An extended physical map of the TOX2 locus of Cochliobolus carbonum required for biosynthesis of HC-toxin. Fungal Genet Biol 35(1):31-8. doi: 10.1006/fgbi.2001.1305.
3. Wight WD et al. (2013) Conservation of the genes for HC-toxin biosynthesis in Alternaria jesenskae. BMC Microbiol 13:165. doi: 10.1186/1471-2180-13-165.
4. Walton JD. (2006) HC-toxin. Phytochemistry 67(14):1406-13. doi: 10.1016/j.phytochem.2006.05.033.

BGC0000370, HC-toxin biosynthetic gene cluster from Bipolaris zeicola. Locus 4. Full MIBiG entry.

Chemical compounds

Compound: HC-toxin
PubChem ID: 107864
SMILES string: Copy to clipboard
Molecular formula: C21H32N4O6
Average molecular mass: 436.509 Da
Molecular activity: Inhibitor
Molecular target: histone deacetylase RPD3-class

Class-specific details

Biosynthetic class(es):
NRP
Other (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
HTS1 (AAA33023.2)
Module 1
A specificity: Proline
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: DCL
Scaffold-modifying domain: Other
Module 2
A specificity: Alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 3
A specificity: D-alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 4
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: DCL

Gene cluster description

HC-toxin (BGC0000370). Gene Cluster 4. Biosynthetic class = NRP. GenBank X92391. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

General MIBiG information on this cluster

Complete gene cluster sequence?incomplete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Jonathan Walton (Michigan State University)

Literature references

1. Ahn JH, Walton JD. (1996) Chromosomal organization of TOX2, a complex locus controlling host-selective toxin biosynthesis in Cochliobolus carbonum. Plant Cell 8(5):887-97. doi: 10.1105/tpc.8.5.887.
2. Ahn JH et al. (2002) An extended physical map of the TOX2 locus of Cochliobolus carbonum required for biosynthesis of HC-toxin. Fungal Genet Biol 35(1):31-8. doi: 10.1006/fgbi.2001.1305.
3. Wight WD et al. (2013) Conservation of the genes for HC-toxin biosynthesis in Alternaria jesenskae. BMC Microbiol 13:165. doi: 10.1186/1471-2180-13-165.
4. Walton JD. (2006) HC-toxin. Phytochemistry 67(14):1406-13. doi: 10.1016/j.phytochem.2006.05.033.

BGC0000370, HC-toxin biosynthetic gene cluster from Bipolaris zeicola. Locus 5. Full MIBiG entry.

Chemical compounds

Compound: HC-toxin
PubChem ID: 107864
SMILES string: Copy to clipboard
Molecular formula: C21H32N4O6
Average molecular mass: 436.509 Da
Molecular activity: Inhibitor
Molecular target: histone deacetylase RPD3-class

Class-specific details

Biosynthetic class(es):
NRP
Other (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
HTS1 (AAA33023.2)
Module 1
A specificity: Proline
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: DCL
Scaffold-modifying domain: Other
Module 2
A specificity: Alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 3
A specificity: D-alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 4
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: DCL

Gene cluster description

HC-toxin (BGC0000370). Gene Cluster 5. Biosynthetic class = NRP. GenBank AF038874. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

General MIBiG information on this cluster

Complete gene cluster sequence?incomplete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Jonathan Walton (Michigan State University)

Literature references

1. Ahn JH, Walton JD. (1996) Chromosomal organization of TOX2, a complex locus controlling host-selective toxin biosynthesis in Cochliobolus carbonum. Plant Cell 8(5):887-97. doi: 10.1105/tpc.8.5.887.
2. Ahn JH et al. (2002) An extended physical map of the TOX2 locus of Cochliobolus carbonum required for biosynthesis of HC-toxin. Fungal Genet Biol 35(1):31-8. doi: 10.1006/fgbi.2001.1305.
3. Wight WD et al. (2013) Conservation of the genes for HC-toxin biosynthesis in Alternaria jesenskae. BMC Microbiol 13:165. doi: 10.1186/1471-2180-13-165.
4. Walton JD. (2006) HC-toxin. Phytochemistry 67(14):1406-13. doi: 10.1016/j.phytochem.2006.05.033.

BGC0000370, HC-toxin biosynthetic gene cluster from Bipolaris zeicola. Locus 6. Full MIBiG entry.

Chemical compounds

Compound: HC-toxin
PubChem ID: 107864
SMILES string: Copy to clipboard
Molecular formula: C21H32N4O6
Average molecular mass: 436.509 Da
Molecular activity: Inhibitor
Molecular target: histone deacetylase RPD3-class

Class-specific details

Biosynthetic class(es):
NRP
Other (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
HTS1 (AAA33023.2)
Module 1
A specificity: Proline
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: DCL
Scaffold-modifying domain: Other
Module 2
A specificity: Alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 3
A specificity: D-alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 4
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: DCL

Gene cluster description

HC-toxin (BGC0000370). Gene Cluster 6. Biosynthetic class = NRP. GenBank AF157629. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

General MIBiG information on this cluster

Complete gene cluster sequence?incomplete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Jonathan Walton (Michigan State University)

Literature references

1. Ahn JH, Walton JD. (1996) Chromosomal organization of TOX2, a complex locus controlling host-selective toxin biosynthesis in Cochliobolus carbonum. Plant Cell 8(5):887-97. doi: 10.1105/tpc.8.5.887.
2. Ahn JH et al. (2002) An extended physical map of the TOX2 locus of Cochliobolus carbonum required for biosynthesis of HC-toxin. Fungal Genet Biol 35(1):31-8. doi: 10.1006/fgbi.2001.1305.
3. Wight WD et al. (2013) Conservation of the genes for HC-toxin biosynthesis in Alternaria jesenskae. BMC Microbiol 13:165. doi: 10.1186/1471-2180-13-165.
4. Walton JD. (2006) HC-toxin. Phytochemistry 67(14):1406-13. doi: 10.1016/j.phytochem.2006.05.033.

BGC0000370, HC-toxin biosynthetic gene cluster from Bipolaris zeicola. Locus 7. Full MIBiG entry.

Chemical compounds

Compound: HC-toxin
PubChem ID: 107864
SMILES string: Copy to clipboard
Molecular formula: C21H32N4O6
Average molecular mass: 436.509 Da
Molecular activity: Inhibitor
Molecular target: histone deacetylase RPD3-class

Class-specific details

Biosynthetic class(es):
NRP
Other (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
HTS1 (AAA33023.2)
Module 1
A specificity: Proline
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: DCL
Scaffold-modifying domain: Other
Module 2
A specificity: Alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 3
A specificity: D-alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 4
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: DCL

Gene cluster description

HC-toxin (BGC0000370). Gene Cluster 7. Biosynthetic class = NRP. GenBank AF169478. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

General MIBiG information on this cluster

Complete gene cluster sequence?incomplete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Jonathan Walton (Michigan State University)

Literature references

1. Ahn JH, Walton JD. (1996) Chromosomal organization of TOX2, a complex locus controlling host-selective toxin biosynthesis in Cochliobolus carbonum. Plant Cell 8(5):887-97. doi: 10.1105/tpc.8.5.887.
2. Ahn JH et al. (2002) An extended physical map of the TOX2 locus of Cochliobolus carbonum required for biosynthesis of HC-toxin. Fungal Genet Biol 35(1):31-8. doi: 10.1006/fgbi.2001.1305.
3. Wight WD et al. (2013) Conservation of the genes for HC-toxin biosynthesis in Alternaria jesenskae. BMC Microbiol 13:165. doi: 10.1186/1471-2180-13-165.
4. Walton JD. (2006) HC-toxin. Phytochemistry 67(14):1406-13. doi: 10.1016/j.phytochem.2006.05.033.

BGC0000370, HC-toxin biosynthetic gene cluster from Bipolaris zeicola. Locus 8. Full MIBiG entry.

Chemical compounds

Compound: HC-toxin
PubChem ID: 107864
SMILES string: Copy to clipboard
Molecular formula: C21H32N4O6
Average molecular mass: 436.509 Da
Molecular activity: Inhibitor
Molecular target: histone deacetylase RPD3-class

Class-specific details

Biosynthetic class(es):
NRP
Other (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactamization

Nonribosomal peptide synthetases:
HTS1 (AAA33023.2)
Module 1
A specificity: Proline
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: DCL
Scaffold-modifying domain: Other
Module 2
A specificity: Alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 3
A specificity: D-alanine
Evidence for specificity: Activity assay
C domain subtype: DCL
Module 4
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: DCL

Gene cluster description

HC-toxin (BGC0000370). Gene Cluster 8. Biosynthetic class = NRP. GenBank KC866372. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

General MIBiG information on this cluster

Complete gene cluster sequence?incomplete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Jonathan Walton (Michigan State University)

Literature references

1. Ahn JH, Walton JD. (1996) Chromosomal organization of TOX2, a complex locus controlling host-selective toxin biosynthesis in Cochliobolus carbonum. Plant Cell 8(5):887-97. doi: 10.1105/tpc.8.5.887.
2. Ahn JH et al. (2002) An extended physical map of the TOX2 locus of Cochliobolus carbonum required for biosynthesis of HC-toxin. Fungal Genet Biol 35(1):31-8. doi: 10.1006/fgbi.2001.1305.
3. Wight WD et al. (2013) Conservation of the genes for HC-toxin biosynthesis in Alternaria jesenskae. BMC Microbiol 13:165. doi: 10.1186/1471-2180-13-165.
4. Walton JD. (2006) HC-toxin. Phytochemistry 67(14):1406-13. doi: 10.1016/j.phytochem.2006.05.033.