BGC0000374: hormaomycin biosynthetic gene cluster from Streptomyces griseoflavus
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Location: 1 - 51,499 nt. (total: 51,499 nt).
This entry is originally from NCBI GenBank HQ542230.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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resistance
TTA codons
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Gene details
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General
Compounds
Genes
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000374
Short description hormaomycin biosynthetic gene cluster from Streptomyces griseoflavus
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
Loci NCBI GenBank: HQ542230.1
Compounds
  • hormaomycin
  • hormaomycin A1
  • hormaomycin A2
  • hormaomycin A3
  • hormaomycin A4
  • hormaomycin A5
  • hormaomycin A6
Species Streptomyces griseoflavus [taxonomy]
References
Chemical products information
hormaomycin [synonyms: takaokamycin]
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C55H69Cl1N10O14
hormaomycin A1
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C55H70N10O14
hormaomycin A2
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C54H67Cl1N10O14
hormaomycin A3
(no structure information available)
C1C1C1H1C1C1H1C1N1C1C1H1C1C1C1C1C1H1C2O1O1N1C1C1H1C1H1C3C1C1C1C1C3C1N1C1C1H1N1C1C1H1C1H1C1C4C1C1C1C1C4N1C1C1H1C1H1C1O2N1C1C1H1C1C1H1C1H1N1O1O1C5N1C1C6C1C1C1Cl1N6O1O1O1O1O1C1C1C1C1O1O1
hormaomycin A4
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C55H72Cl1N9O12
hormaomycin A5
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C55H75Cl1N8O10
hormaomycin A6
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C59H75Cl1N10O16
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AEH41781.1
 656 - 1624 (-) HrmA
  • Regulation
Other in vivo study
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  • AEH41782.1
 2626 - 3219 (+) HrmB
  • Regulation
Other in vivo study
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  • AEH41802.1
 3257 - 4333 (+) HrmC
  • Precursor biosynthesis
Sequence-based prediction
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  • AEH41801.1
 4336 - 5232 (+) HrmD
  • Precursor biosynthesis
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  • AEH41783.1
 5306 - 6286 (-) HrmE
  • Precursor biosynthesis
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  • AEH41784.1
 6270 - 6824 (-) HrmF
  • Precursor biosynthesis
Activity assay
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  • AEH41785.1
 6869 - 8590 (-) HrmG
  • Precursor biosynthesis
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  • AEH41786.1
 8916 - 9338 (+) HrmH
  • Regulation
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  • AEH41787.1
 9386 - 10435 (+) HrmI
  • Precursor biosynthesis
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  • AEH41788.1
 10449 - 11132 (+) HrmJ
  • Precursor biosynthesis
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  • AEH41789.1
 11145 - 12728 (+) HrmK
  • Scaffold biosynthesis
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  • AEH41790.1
 12725 - 13000 (+) HrmL
  • Scaffold biosynthesis
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  • AEH41791.1
 13039 - 14205 (+) HrmM
  • Precursor biosynthesis
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  • AEH41792.1
 14230 - 15399 (+) HrmN
  • Precursor biosynthesis
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  • AEH41793.1
 15504 - 31262 (+) HrmO
  • Scaffold biosynthesis
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  • AEH41794.1
 31259 - 41674 (+) HrmP
  • Scaffold biosynthesis
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  • ACE80255.1
 41671 - 43017 (+) HrmQ
  • Precursor biosynthesis
Activity assay
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  • AEH41800.1
 43074 - 43292 (+) HrmR
  • Scaffold biosynthesis
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  • AEH41795.1
 43304 - 44293 (+) HrmS
  • Precursor biosynthesis
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  • AEH41796.1
 44408 - 45313 (+) HrmT
  • Precursor biosynthesis
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  • AEH41797.1
 45544 - 46593 (+) HrmU
  • Transport
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  • AEH41798.1
 46906 - 47376 (+) HrmV
  • Transport
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  • AEH41799.1
 47373 - 49064 (+) HrmW
  • Transport
Sequence-based prediction
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NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
Thioesterase genes
  • AEH41794.1 (Type I)
NRP-synthases
Gene Modules
AEH41790.1
Module 0
AEH41793.1
Module 1
Specificity: 3-(2-nitrocyclopropylalanine)
Evidence for specificity:
  • Activity assay
Condensation domain type: LCL
Module 2
Specificity: threonine
Evidence for specificity:
  • Activity assay
Condensation domain type: LCL
Module 3
Specificity: R-beta-methylphenylalanine / phenylalanine / 2-fluorophenylalanine / 3-fluorophenylalanine
Evidence for specificity:
  • Structure-based inference
  • Homology
  • Feeding study
Condensation domain type: LCL
Module 4
Specificity: 3-(2-nitrocyclopropylalanine)
Evidence for specificity:
  • Activity assay
Condensation domain type: LCL
AEH41794.1
Module 5
Specificity: R-beta-methylphenylalanine / phenylalanine / 2-fluorophenylalanine / 3-fluorophenylalanine / threonine / tryptophan / isoleucine / valine / alpha-methylphenylalanine
Evidence for specificity:
  • ATP-PPi exchange assay
  • Feeding study
Condensation domain type: LCL
Module 6
both the activity assay and the nonribosomal code for HrmP2A suggest that Val is incorporated into hormaomycin although Ile is the amino acid present in hormaomycin.

Specificity: valine
Evidence for specificity:
  • Activity assay
Condensation domain type: LCL
Module 7
Specificity: 4S-propenylproline
Evidence for specificity:
  • Activity assay
Condensation domain type: LCL
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: CUIZDUBUBVAF27VHF4OZXTI3, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Removed gene names of 'No gene ID'
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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