Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000374.1:
Cluster 1NRP151499

BGC0000374, hormaomycin biosynthetic gene cluster from Streptomyces griseoflavus. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: hormaomycin
SMILES string: Copy to clipboard
Molecular formula: C55H69ClN10O14
Exact molecular mass: 1151.4546 Da ([M+Na]+)
Molecular activity: Antibacterial, Signalling, Other

Class-specific details

Biosynthetic class(es):
NRP
Cyclic depsipeptide (cyclic)
Thioesterase type:
Type I
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
No gene ID (AEH41790.1)
Module 0
A specificity: Unknown
Evidence for specificity: Unknown
C domain subtype: N/A
No gene ID (AEH41793.1)
Module 1
A specificity: (3-Ncp)Ala
Evidence for specificity: Activity assay
C domain subtype: LCL
Module 2
A specificity: Threonine
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: LCL
Module 3
A specificity: (beta-Me)Phe
Evidence for specificity: Activity assay
C domain subtype: LCL
Module 4
A specificity: (3-Ncp)Ala
Evidence for specificity: Activity assay
AA is epimerized
C domain subtype: LCL
No gene ID (AEH41794.1)
Module 5
A specificity: (beta-Me)Phe
Evidence for specificity: Activity assay
C domain subtype: LCL
Module 6
A specificity: Valine
Evidence for specificity: Activity assay
C domain subtype: LCL
Module 7
A specificity: (4-Pe)Pro
Evidence for specificity: Activity assay
C domain subtype: LCL

Gene cluster description

hormaomycin (BGC0000374). Gene Cluster 1. Biosynthetic class = NRP. GenBank HQ542230, positions 1-51499. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Sequence-based prediction, Other
MIxS-compliance:Unknown
Contact for this cluster:Eric J. N. Helfrich (Institute of Microbiology, Eidgenoessische Technische Hochschule (ETH) Zurich, Piel lab)

Literature references

1. Hofer I et al. (2011) Insights into the biosynthesis of hormaomycin, an exceptionally complex bacterial signaling metabolite. Chem Biol 18(3):381-91. doi: 10.1016/j.chembiol.2010.12.018.
2. Cai X et al. (2013) Manipulation of regulatory genes reveals complexity and fidelity in hormaomycin biosynthesis. Chem Biol 20(6):839-46. doi: 10.1016/j.chembiol.2013.04.018.
3. Max Crusemann, Christoph Kohlhaas, Jorn Piel. (2013) Evolution-guided engineering of nonribosomal peptide synthetase adenylation domains. Chem. Sci., vol. 4, no. 3, pp. 1041-1045
4. Ellen Rossner, Axel Zeeck, Wilfried A. Konig. (1990) Elucidation of the Structure of Hormaomycin. Angewandte Chemie International Edition in English, vol. 29, no. 1, pp. 64-65