BGC0000380 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000380 (Sorangium cellulosum)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,T1PKS	1	80,175	Leupyrrin A1/Leupyrrin A2/Leupyrrin B1/Leupyrrin B2/Leupyrrin C/Leupyrrin D	NRPS:Type I+PKS:Type I	100%

BGC0000380 - Region 1 - NRPS,T1PKS

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Location: 1 - 80,175 nt. (total: 80,175 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 80,176
Label		BGC0000380

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000380	2.00	NRP, Polyketide	leupyrrin	Sorangium cellulosum
BGC0000016	0.67	Other	amphotericin B	Streptomyces nodosus
BGC0002391	0.66	Terpene	zealexin B2, zealexin C2	Zea mays
BGC0000996	0.66	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0001043	0.66	NRP, Polyketide	SGR PTMs, SGR PTM Compound b, SGR PTM Compound c, SGR PTM Compound d	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002365	0.66	Other, Polyketide	10-epi-HSAF, 10-epi-3-deOH-HSAF, 10-epi-maltophilin, 10-epi-xanthobaccin C, 10-epi-hydroxymaltophilin, 10-epi-FI-2	Streptomyces sp.
BGC0001726	0.65	NRP, Polyketide	pactamides	Streptomyces pactum
BGC0001890	0.64	NRP	amphi-enterobactin 1, amphi-enterobactin 2, amphi-enterobactin 3, amphi-enterobactin 4	Vibrio campbellii ATCC BAA-1116
BGC0000894	0.64	Other	citrinin	Monascus aurantiacus
BGC0002509	0.64	NRP	montamide A, capsimycin, clifednamide A, frontalamide A, combamide A	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000380	1.00	NRP, Polyketide	leupyrrin	Sorangium cellulosum
BGC0000016	0.67	Other	amphotericin B	Streptomyces nodosus
BGC0002391	0.66	Terpene	zealexin B2, zealexin C2	Zea mays
BGC0000996	0.66	NRP, Polyketide	frontalamide B, frontalamide A	Streptomyces sp. SPB78
BGC0001043	0.66	NRP, Polyketide	SGR PTMs, SGR PTM Compound b, SGR PTM Compound c, SGR PTM Compound d	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002365	0.66	Other, Polyketide	10-epi-HSAF, 10-epi-3-deOH-HSAF, 10-epi-maltophilin, 10-epi-xanthobaccin C, 10-epi-hydroxymaltophilin, 10-epi-FI-2	Streptomyces sp.
BGC0001726	0.66	NRP, Polyketide	pactamides	Streptomyces pactum
BGC0001890	0.66	NRP	amphi-enterobactin 1, amphi-enterobactin 2, amphi-enterobactin 3, amphi-enterobactin 4	Vibrio campbellii ATCC BAA-1116
BGC0000894	0.66	Other	citrinin	Monascus aurantiacus
BGC0002509	0.65	NRP	montamide A, capsimycin, clifednamide A, frontalamide A, combamide A	Streptomyces sp.

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 647 - 73011:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Cys - ccmal) + (Me-ccmal) + (ccmal) + (Me-ccmal) + (Pro)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

leu5

AMP-binding (10..429): X

nrpys: Pca

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLLYLALVCK
Nearest Stachelhaus code(s):: Pca DLLYLALVCK (100% 8Å match); Pro DLLYLALVCK (82% 8Å match)
Stachelhaus code match:: 100% (strong)

leuA

Search NORINE for peptide: strict or relaxed

AMP-binding (544..944): Cys

nrpys: Cys

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: DMFNFGVLWK
Nearest Stachelhaus code(s):: Cys DMFNFGVLWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

PKS_AT (1675..1973): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 142.9
inactive: 99.6
Methoxymalonyl-CoA: 76.1
Methylmalonyl-CoA: 59.9
Propionyl-CoA: 43.1
Isobutyryl-CoA: 38.3
2-Methylbutyryl-CoA: 25.0
CHC-CoA: 24.6
Ethylmalonyl-CoA: 24.2
fatty_acid: 18.6
Acetyl-CoA: 18.6
Benzoyl-CoA: 17.9
trans-1,2-CPDA: 14.5
3-Methylbutyryl-CoA: 7.5

leuB

PKS_AT (574..853): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; Methylmalonyl-CoA: 66.7%; inactive: 62.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 152.7
inactive: 101.9
Methoxymalonyl-CoA: 79.5
Methylmalonyl-CoA: 79.3
Propionyl-CoA: 52.8
Isobutyryl-CoA: 50.1
Ethylmalonyl-CoA: 47.1
Benzoyl-CoA: 38.4
2-Methylbutyryl-CoA: 34.1
3-Methylbutyryl-CoA: 31.8
Acetyl-CoA: 25.1
trans-1,2-CPDA: 18.0
fatty_acid: 17.8
CHC-CoA: 17.2

leuC

PKS_AT (568..863): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 91.7%; inactive: 83.3%; Propionyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 172.2
inactive: 113.8
Methoxymalonyl-CoA: 57.4
Methylmalonyl-CoA: 48.8
Propionyl-CoA: 44.6
Benzoyl-CoA: 29.6
fatty_acid: 28.4
Isobutyryl-CoA: 26.1
Ethylmalonyl-CoA: 25.9
Acetyl-CoA: 23.0
2-Methylbutyryl-CoA: 20.4
trans-1,2-CPDA: 13.3
CHC-CoA: 8.6
3-Methylbutyryl-CoA: 0.0

leuD

PKS_AT (560..860): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 70.8%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 153.3
inactive: 114.1
Methylmalonyl-CoA: 70.8
Methoxymalonyl-CoA: 68.6
Isobutyryl-CoA: 38.9
Benzoyl-CoA: 29.1
Propionyl-CoA: 27.4
Ethylmalonyl-CoA: 26.5
2-Methylbutyryl-CoA: 22.8
fatty_acid: 13.5
3-Methylbutyryl-CoA: 12.6
trans-1,2-CPDA: 11.8
Acetyl-CoA: 9.1
CHC-CoA: 0.0

leuE

Search NORINE for peptide: strict or relaxed

AMP-binding (535..937): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAQVVK
Nearest Stachelhaus code(s):: Pro DVQFIAQVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

leu17

AMP-binding (47..471): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: TACLLLMPLK
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)