LOCUS       BGC0000382             19400 bp    DNA     linear   BCT 10-FEB-2015
DEFINITION  Streptomyces toxytricini strain NRRL 15443 lipstatin biosynthetic
            gene cluster, complete sequence.
ACCESSION   BGC0000382
VERSION     BGC0000382
KEYWORDS    .
SOURCE      Streptomyces toxytricini
  ORGANISM  Streptomyces toxytricini
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:30:58
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..19400
                     /culture_collection="NRRL:15443"
                     /db_xref="taxon:67369"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces toxytricini"
                     /strain="NRRL 15443"
     protocluster    1..19400
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[6767:11513]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      6768..11513
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     cand_cluster    1..19400
                     /SMILES="NC(CC(C)C)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="single"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..19400
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000382"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..19400
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            456..1580
                     /gene="lstA"
     CDS             456..1580
                     /codon_start=1
                     /gene="lstA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) fabH"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1084:3-oxoacyl-(acyl carrier protein) synthase III
                     (Score: 163.1; E-value: 1.4e-49)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to beta-ketoacyl-ACP synthase III (KASIII)"
                     /product="LstA"
                     /protein_id="AIT38298.1"
                     /sec_met_domain="fabH (E-value: 1e-26, bitscore: 83.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTTERRSRIEALGAFLPAGRETNDELRAKVPNLGDADVRRITGI
                     AERRVHDPDPAAGEDSFGMALAAARDCLAVSRHRAADLDVVISASITRVKDGSRFHFEP
                     SFAGMLAKELGARPAISFDVSNACAGMMTGVWLLDRMIRSGAVRSGMVVSGEQATRVAR
                     TAARELRDSYDPQFASLSVGDSAAAVVLDESTDPADRIHYIELMTCAAYSHLCLGMPSD
                     RSQGIGLYTDNKKMHDRERLKLWPRFHEDFLAKNGRRFEDEEFDHIIQHQVGTRFIEYA
                     NRTAEAEFAAPMPPSLQVVEQYGNTATTSHFLTLRDHLRRTRGAGATGTGTGPGSGPGA
                     GPAREAAGAKYLLVPAASGLVTGALSATVTHAGA"
     misc_feature    456..19364
                     /note="lipstatin biosynthetic gene cluster"
     gene            1587..2453
                     /gene="lstB"
     CDS             1587..2453
                     /codon_start=1
                     /gene="lstB"
                     /note="similar to beta-ketoacyl-ACP synthase III (KASIII)"
                     /product="LstB"
                     /protein_id="AIT38299.1"
                     /transl_table=11
                     /translation="MGIVITASATATHTDPGTPASAVDLAGRAARRCLAHARVSPSGVG
                     VLVNVGVYRENNTFEPALAALVQKETGINPDYLADPQPAAGFSFDLMDGACGVLSAVQA
                     GQSLLSTGTTERLLITAADVHPGGDASRDPDYPYADLAGAFLLERDADPDTGFGPVRHY
                     GGGDRPTDVAGYLDLDTMGSGGRSRITVHRTPGHEQRTGELAAAAVAAYTGEFGLDAGR
                     TLVIGPDAPAGVGDGPGGGRPHTAAPVLGYLHALESARPEGVDTLLFVTAGAGPRAAVA
                     SYRPQGW"
     gene            2459..5083
                     /gene="lstC"
     CDS             2459..5083
                     /NRPS_PKS="Domain: AMP-binding (27-488). E-value: 1.5e-68.
                     Score: 223.1. Matches aSDomain:
                     nrpspksdomains_lstC_AMP-binding.1"
                     /codon_start=1
                     /gene="lstC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     220.5; E-value: 5.3e-67)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to acyl-CoA synthetase"
                     /product="LstC"
                     /protein_id="AIT38300.1"
                     /sec_met_domain="AMP-binding (E-value: 7.5e-64, bitscore:
                     205.6, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATTTATPAAARPAAADDLGAHSLAGLLERNARAFPDKPAVIHPA
                     AGPRRDGASPAYRTLTYGRLQQAVEELAAGLTRAGITKGTKTVLMAPPGPELFALAFAL
                     FRVGAVPVVVDPGMGVRRMLHCYRTVGAEAFIGPPLAHAARLLGRRAFAGIRVPVTLGR
                     HRLGRARTLAAVRALGARGGAAAPVAAGRDDLLMIGFTTGSTGPAKGVEYTHRMALSAA
                     RQIEAVHGRTRDDTSLVTLPFYGVLDLVYGSTLVLAPLAPSRVAQADPALVVDALERFR
                     VTTMFASPALLGPLAAHLAAAAPGRHPLPDLRCVVGGGAPVPDTTVAALRRALDPRARI
                     HVTYGATEALPITSIEAEELLGPEDGGEGGGSGVGGAGSGGTAARAAEGAGTCVGRPVP
                     GIGLAVLPVTDGPLTGSVPHLPTGRVGEIAVRGDCVSPRYHHSPDADRLHKVPDDTDPA
                     GPAWHRTGDLGYLDDDGRLWFCGRSAQRVRTGTGDLHTVRCEGVFNAHPQVRRTALVGI
                     PASPDSGWGRGGRTTTRSGTGSGGTGTARGATESSVAAGNGNTSTAAAPTTATDNGPAH
                     SATPPCETTGNGTPRRPTPARVSAVSAPAHSATTVSGSSGRAAAVSGSAASAAPGSETV
                     VGGSAGSTSAPGATTAGARAGSAAAGMAAEGSGTARSRTGGRGSAGDGTALGGSATAAP
                     PGVAPGGVPADPRRNRLRPVVCVETVDEDLDEAAWQRLTAELRTLARTHAPTTDLQEFL
                     HHPGFPVDIRHNAKIGREELARWAERRLTPPTPLTPRQRAARIVPLAGWAYLVGGAVWA
                     AAFGVPEARLPRLLWWADAVLSTAGHAVQIPLALPRARTAGIGRPAAVGLTMLYGATWW
                     RQL"
     aSDomain        2540..3922
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_lstC_AMP-binding.1"
                     /evalue="1.50E-68"
                     /label="lstC_AMP-binding.1"
                     /locus_tag="lstC"
                     /protein_end="488"
                     /protein_start="27"
                     /score="223.1"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="LERNARAFPDKPAVIHPAAGPRRDGASPAYRTLTYGRLQQAVEEL
                     AAGLTRAGITKGTKTVLMAPPGPELFALAFALFRVGAVPVVVDPGMGVRRMLHCYRTVG
                     AEAFIGPPLAHAARLLGRRAFAGIRVPVTLGRHRLGRARTLAAVRALGARGGAAAPVAA
                     GRDDLLMIGFTTGSTGPAKGVEYTHRMALSAARQIEAVHGRTRDDTSLVTLPFYGVLDL
                     VYGSTLVLAPLAPSRVAQADPALVVDALERFRVTTMFASPALLGPLAAHLAAAAPGRHP
                     LPDLRCVVGGGAPVPDTTVAALRRALDPRARIHVTYGATEALPITSIEAEELLGPEDGG
                     EGGGSGVGGAGSGGTAARAAEGAGTCVGRPVPGIGLAVLPVTDGPLTGSVPHLPTGRVG
                     EIAVRGDCVSPRYHHSPDADRLHKVPDDTDPAGPAWHRTGDLGYLDDDGRLWFCGRSAQ
                     RVR"
     CDS_motif       3719..3793
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_lstC_0001"
                     /evalue="5.10E-04"
                     /label="NRPS-A_a6"
                     /locus_tag="lstC"
                     /protein_end="445"
                     /protein_start="420"
                     /score="12.5"
                     /tool="antismash"
                     /translation="PTGRVGEIAVRGDCVSPRYHHSPDA"
     gene            5080..6771
                     /gene="lstD"
     CDS             5080..6771
                     /NRPS_PKS="Domain: NAD_binding_4 (4-216). E-value: 5e-25.
                     Score: 79.9. Matches aSDomain:
                     nrpspksdomains_lstD_NAD_binding_4.1"
                     /codon_start=1
                     /gene="lstD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score:
                     221.2; E-value: 3.4e-67)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to dehydrogenase"
                     /product="LstD"
                     /protein_id="AIT38301.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.8e-09, bitscore:
                     26.3, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 1.7e-08,
                     bitscore: 23.7, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 1.2e-25, bitscore:
                     79.9, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILITGATGFLGGHLADACLRSGHGVRALVRPGSNTDRLRALPG
                     VELVTGDLTRPDSLRRAADGCEAVLHSAARVVDHGTRAQFTEANVTGTLRLMDAARAAG
                     VRRFVFVSSPSALMHLREGDRLGIDETTPYPTRWFNDYCATKAVAEQHVLAADTAGFTT
                     CALRPRGIWGPRDHAGFLPRLIGALHAGRLPDLSGGKHVLVSLCHVDNAVDACLRAAVS
                     APAERIGGRAYFVADAETTDLWPFLADVAARLGCPPPAPRIPLPAGRALAAAVETAWRL
                     RPDAAARARSSPPLSRYMMALLTRSSTYDTTAARRDLGYTPVRTQEDGLRDLVRWVASQ
                     GGVASWTAPRPHPAHTHTPDATPHAPARAPHPPMPEPPAAATPAPPPKAEHRPALPRPR
                     SSPEADSTEQPFPHPADATDTPPVSGPAPGPVSVPAPDRTPAPSGSSRTAGDAPACRAG
                     QASGPAPAPVRGPADARSAATGRGPRPVRGSAEQREHRDPSLRASGKPGSDGSGAPADT
                     RPNHDPTRAEAARPGDAGRGMAPEGDTARRGSTDPAGPAGREDTSR"
     aSDomain        5092..5727
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_lstD_NAD_binding_4.1"
                     /evalue="5.00E-25"
                     /label="lstD_NAD_binding_4.1"
                     /locus_tag="lstD"
                     /protein_end="216"
                     /protein_start="4"
                     /score="79.9"
                     /tool="antismash"
                     /translation="ITGATGFLGGHLADACLRSGHGVRALVRPGSNTDRLRALPGVELV
                     TGDLTRPDSLRRAADGCEAVLHSAARVVDHGTRAQFTEANVTGTLRLMDAARAAGVRRF
                     VFVSSPSALMHLREGDRLGIDETTPYPTRWFNDYCATKAVAEQHVLAADTAGFTTCALR
                     PRGIWGPRDHAGFLPRLIGALHAGRLPDLSGGKHVLVSLCHVDNAVDAC"
     gene            6768..11513
                     /gene="lstE"
     CDS             6768..11513
                     /NRPS_PKS="Domain: AMP-binding (824-1224). E-value:
                     1.9e-84. Score: 275.5. Matches aSDomain:
                     nrpspksdomains_lstE_AMP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (1499-1542). E-value:
                     1.7e-09. Score: 30.0. Matches aSDomain:
                     nrpspksdomains_lstE_PP-binding.1"
                     /codon_start=1
                     /gene="lstE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     310.9; E-value: 2.1e-94)"
                     /gene_kind="biosynthetic"
                     /note="nonribosomal peptide synthetase (NRPS)"
                     /product="LstE"
                     /protein_id="AIT38302.1"
                     /sec_met_domain="AMP-binding (E-value: 8.1e-94, bitscore:
                     304.3, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.1e-10, bitscore:
                     30.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTSTPNPPGTSEPQRVSRSAAASDLPSPTGTPGSPGAPGASVPR
                     ASSEASGPAAAAGRPSGALPAPEPSAAVPGASTSPQVPRSPAPAAPGTASQAGTPGPAE
                     DAGSPAASSACTAPVSPGSYLASGNTVSPSPSPSALPATPGTTVAPGAAGGPELPEAVG
                     SATLPGASGTPEARRSSTAGGGSGPHSSDATAAAAYRRPVSPTERLYLAAGEPRGAMAL
                     RIVVEGDGLPDAGQLHAAVARAAETCPGSRLVRRGPLWAADGPPPHIVHAAAAEGPFDV
                     SDPRTARLLTGRSGPGDQPGCELLVVPGAGGARSTLVFSASHALMDGHGALTWVREVFR
                     ALRGEAARPAADPSTDLGVLRRLAADRGGRPRRRPAPLPGRRSPLGPASATGSASQTLW
                     LRRTLPGRHPALTARLAQAVADTARLDTRAMVPVDLRRHLPHLAAATGNLTLPLFLDLR
                     PGQDWAAAHTRLLTALAAGGELAAGFESGPVSRLPQPAVAALLRAAQAAAVRADRHLAS
                     AVVSHLGRLDTGELSGGGFTAATAYALPVHAPLVPLSFAVAETPYAIEITLGVRATDPS
                     LPHRASAFLDSVLAVLAADGAGPRTNTTPARTAASDPSATTAESGPADASAPASTRTAP
                     ADVLTPSASAGASGVCAPPGSAGEAAPDASSAPAASVGAYPPVGASAASDASTQIAPAD
                     ALTPAASAGGFPPDARSVPAASVGAYVPVDASDASTCSAPAGASAPTTSADASGACVAS
                     ASADLPAPVVLGDTSDLAEVSGASGRTAQADACTPSVPADGSAPVTPADASDASARTVP
                     ADATAAGTVVDLFRAQAARTPAAVALDGPAGPVTYAELDRRSDAVAAELVRRGTARRDL
                     VGLVVDRTPEGVAGLWGILKAGAAYVPLDPAHPGPRIRGVLASCGVRLTLTRRHLAAHL
                     AEFVPGALLAVDDVPTPAQPPFAPVATGPAPGDLAYVMHTSGSTGHPKGVLIEHRGLVA
                     FARWMTDLCRVGEGTRFGFASSYAFDISCFPLFLPLLAGGTAVLAPGAPSPATLRALVG
                     EHRADTLALTPSHLALLDGDGGGPLSTLLLGGEELTPAAVRSAYAAFGTGCRLINGYGP
                     TEATVACLAHVVDDTETGPSLPLGTPGPDLRVDLVAEDGASVGTGPDDVGRVGEIVVHG
                     IQVARGYHGSVSAEPDGTVGPFSTGPDGLRSYRTGDLGRRLPGGGIAFAGRADGQVKVA
                     GHRIEPAEITAALEAHPGVARAAVTTRRRPGGRGLVLCAYAVPSTGTATTGPTAAPAAG
                     AGGPADPGTAESGPAYSGPAAAGPADSGSAATGTATTGLAITGTVAAGGPADPGTADAG
                     SARTGRAGTGRFDSGPAGSGSAVTGRSGSGSAVTGAVGSGPAATGSADFGRAATDPAGS
                     GPSDPRSGPPVPPAGAPSAAELRAHLAGILPRHLVPAHILTVPGLPTTVSGKTDFDALP
                     DPFAGTAPLPAEPDGPAAPHDTYDAAPDPRSSRKALRSRVARHWATVLGIDAAALTADS
                     DFQALGGDSLALVEMLSAVSADLLTPDETRRFTGDLTVLVRDLTLDRVCDRLAAVREGT
                     TA"
     CDS_motif       8532..8558
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_lstE_0001"
                     /evalue="9.90E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="lstE"
                     /protein_end="597"
                     /protein_start="588"
                     /score="-3.2"
                     /tool="antismash"
                     /translation="LAVLAADGA"
     aSDomain        9240..10439
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_lstE_AMP-binding.1"
                     /evalue="1.90E-84"
                     /label="lstE_AMP-binding.1"
                     /locus_tag="lstE"
                     /protein_end="1224"
                     /protein_start="824"
                     /score="275.5"
                     /specificity="substrate consensus: Leu"
                     /tool="antismash"
                     /translation="FRAQAARTPAAVALDGPAGPVTYAELDRRSDAVAAELVRRGTARR
                     DLVGLVVDRTPEGVAGLWGILKAGAAYVPLDPAHPGPRIRGVLASCGVRLTLTRRHLAA
                     HLAEFVPGALLAVDDVPTPAQPPFAPVATGPAPGDLAYVMHTSGSTGHPKGVLIEHRGL
                     VAFARWMTDLCRVGEGTRFGFASSYAFDISCFPLFLPLLAGGTAVLAPGAPSPATLRAL
                     VGEHRADTLALTPSHLALLDGDGGGPLSTLLLGGEELTPAAVRSAYAAFGTGCRLINGY
                     GPTEATVACLAHVVDDTETGPSLPLGTPGPDLRVDLVAEDGASVGTGPDDVGRVGEIVV
                     HGIQVARGYHGSVSAEPDGTVGPFSTGPDGLRSYRTGDLGRRLPGGGIAFAGRADGQVK
                     V"
     aSModule        9240..11393
                     /complete
                     /domains="nrpspksdomains_lstE_AMP-binding.1"
                     /domains="nrpspksdomains_lstE_PP-binding.1"
                     /locus_tags="lstE"
                     /monomer_pairings="Leu -> Leu"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       9426..9467
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_lstE_0002"
                     /evalue="4.00E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="lstE"
                     /protein_end="900"
                     /protein_start="886"
                     /score="19.8"
                     /tool="antismash"
                     /translation="WGILKAGAAYVPLD"
     CDS_motif       9660..9719
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_lstE_0003"
                     /evalue="6.60E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="lstE"
                     /protein_end="984"
                     /protein_start="964"
                     /score="27.9"
                     /tool="antismash"
                     /translation="AYVMHTSGSTGHPKGVLIEH"
     CDS_motif       10080..10109
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_lstE_0004"
                     /evalue="2.40E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="lstE"
                     /protein_end="1114"
                     /protein_start="1104"
                     /score="7.7"
                     /tool="antismash"
                     /translation="YGPTEATVAC"
     CDS_motif       10233..10292
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_lstE_0005"
                     /evalue="5.70E-07"
                     /label="NRPS-A_a6"
                     /locus_tag="lstE"
                     /protein_end="1175"
                     /protein_start="1155"
                     /score="21.9"
                     /tool="antismash"
                     /translation="VGRVGEIVVHGIQVARGYHG"
     CDS_motif       10407..10469
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_lstE_0006"
                     /evalue="5.70E-09"
                     /label="NRPS-A_a8"
                     /locus_tag="lstE"
                     /protein_end="1234"
                     /protein_start="1213"
                     /score="28.2"
                     /tool="antismash"
                     /translation="FAGRADGQVKVAGHRIEPAEI"
     aSDomain        11265..11393
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_lstE_PP-binding.1"
                     /evalue="1.70E-09"
                     /label="lstE_PP-binding.1"
                     /locus_tag="lstE"
                     /protein_end="1542"
                     /protein_start="1499"
                     /score="30.0"
                     /tool="antismash"
                     /translation="RVARHWATVLGIDAAALTADSDFQALGGDSLALVEMLSAVSAD"
     gene            11510..12202
                     /gene="lstF"
     CDS             11510..12202
                     /codon_start=1
                     /gene="lstF"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1228:methionyl-tRNA formyltransferase (Score: 87;
                     E-value: 1.8e-26)"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to formyltransferase"
                     /product="LstF"
                     /protein_id="AIT38303.1"
                     /transl_table=11
                     /translation="MIFVGEGALLRRAVSHAAARGHRVDLVCSPDPLDAAAAAPGTPHL
                     AAPDVNAAAADLAAACTDGIVWSVNNRQLFRAPVLSLGLRIVNVHNGLLPQHRGLPSAA
                     VVFALLHGDTEYGATVHEVDAGIDTGPVLAEHRFPIGPADRFHEVMLRGVRACQTLFER
                     TLPAVAAGERPRAAPPDAARPSGYYGRRALARLGEHRDAPSFVRATDLGPFAAHAPEVA
                     EALHRVRP"
     CDS             12714..13334
                     /codon_start=1
                     /note="Orf1"
                     /product="hypothetical protein"
                     /protein_id="AIT38304.1"
                     /transl_table=11
                     /translation="MRRLVYYIATTLDGFIAGPDGADPTGPNGFWPIAEDYLQHLVAEY
                     PETLPVQARQALSVTAEGTRFDTVLEGRRTYEIGLAAGIPDAYPHLRHIVFSRTLTELP
                     APAVELVSGDPVATVRELKQQDGKDIWLIGGAELAGSLYAEIDMLVLKVAPLTLGDGIP
                     LFSRKAAFDPRTWTLTDHTVLKSGAAFLTYTRPTDTDTDTDTA"
     CDS             13464..14072
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057:TetR family
                     transcriptional regulator (Score: 77.7; E-value: 2.5e-23)"
                     /gene_kind="regulatory"
                     /note="Orf2"
                     /product="hypothetical protein"
                     /protein_id="AIT38305.1"
                     /transl_table=11
                     /translation="MARPRGVEDAVILRAAVDVIGRSGPGGLTLAAVAREVGLVPGTLV
                     QRFGSKRGLLLALAEQAAREAEDLPVRARQGHDSPLAALEALLVSSTEHMATPEAFAHH
                     LGFLCIDLTDPEFHRHALAVHRAQQRAVEALLGEAAGAGELRPRTDTAALAATVQAAAA
                     GAGLVWALDRSGSLADRVRRELAAVLRAHQPHHPRPEER"
     CDS             14069..14989
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     90.4; E-value: 2.2e-27)"
                     /gene_kind="biosynthetic-additional"
                     /note="Orf3"
                     /product="hypothetical protein"
                     /protein_id="AIT38306.1"
                     /transl_table=11
                     /translation="MTYATPARPLAGKVALVAGGTRGGGRGTAVALGAAGATVYVTGRS
                     SRTAGRSPLDRPETVEETAARVTAAGGLGIPVRVDHSDPDEVRALTARIGAEQSGRLDI
                     LVNSVWGGDPLTDWEHPLWEQDLDTGLRLLRQAVETHIITSRHALPLMVAQGSGLVVEV
                     TDGNTARYRGSFFYDLAKSAVIRLAFAQAAELREHGVAAVAVTPGFLRSEAVLDHFGVT
                     EDTWRDAVARDPDFAHSESPAYLGRAIAALAADPDVLRWTGRALATWSLYKEYGFTDTD
                     GTQPDFAAHWADALEPVHGPLGEPL"
     CDS             15136..15942
                     /NRPS_PKS="Domain: Thioesterase (36-250). E-value: 2.3e-44.
                     Score: 144.3. Matches aSDomain:
                     nrpspksdomains_AIT38307.1_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1004:thioesterase (Score: 220.9; E-value: 2.1e-67)"
                     /gene_kind="biosynthetic-additional"
                     /note="Orf4"
                     /product="hypothetical protein"
                     /protein_id="AIT38307.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 5.7e-11, bitscore:
                     33.3, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQDADRWLKRFTPASSPATGRTPGTAALTPLTRIRSRVVCLPHAG
                     GAAPAYQPWARHLPADTELLAVQYPGRQERLGEPCADTLGELADAVAAALAALPVLPTV
                     VFGHSMGSLVAYETVLRLARHAPQALPVRLFVSGAPAPAHDRTNLPAPDDASLLAYVRN
                     QGAAQPEVYDIPELRELLMPSLRGDFALLARYRPARPPARVPVPVTALGGDSDAGCPPE
                     SLATWADATTADFASRVFPGGHFYLQGREDTLAGLVTTALRAAAAR"
     aSDomain        15244..15885
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AIT38307.1_Thioesterase.1"
                     /evalue="2.30E-44"
                     /label="AIT38307.1_Thioesterase.1"
                     /locus_tag="AIT38307.1"
                     /protein_end="250"
                     /protein_start="36"
                     /score="144.3"
                     /tool="antismash"
                     /translation="RVVCLPHAGGAAPAYQPWARHLPADTELLAVQYPGRQERLGEPCA
                     DTLGELADAVAAALAALPVLPTVVFGHSMGSLVAYETVLRLARHAPQALPVRLFVSGAP
                     APAHDRTNLPAPDDASLLAYVRNQGAAQPEVYDIPELRELLMPSLRGDFALLARYRPAR
                     PPARVPVPVTALGGDSDAGCPPESLATWADATTADFASRVFPGGHFYLQGR"
     CDS_motif       15439..15501
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AIT38307.1_0001"
                     /evalue="4.70E-05"
                     /label="NRPS-te1"
                     /locus_tag="AIT38307.1"
                     /protein_end="122"
                     /protein_start="101"
                     /score="16.0"
                     /tool="antismash"
                     /translation="PTVVFGHSMGSLVAYETVLRL"
     CDS             complement(15965..16858)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014:LysR family
                     transcriptional regulator (Score: 176.9; E-value: 9e-54)"
                     /gene_kind="regulatory"
                     /note="Orf5"
                     /product="hypothetical protein"
                     /protein_id="AIT38308.1"
                     /transl_table=11
                     /translation="MELRHLRALDAVAAAGTVTAAAARLHITQPALSRTLAQLEARVGV
                     RLVDRSSRRPALTAAGTTLLGHARAILAHLDAALADTREVSRPLRLGYTCAVLGQQTVP
                     LLRSWRRTHPHVPLEVVRHDSSTAGLATGDADLAVLRTVPADRRIRTEALYTEGRVAAL
                     PDDDPLAGRDQVLLAELTAGRDLPLALWPETGTASPELWPAGRRPARTVEVRNVDEWLN
                     LIAIGGAFGVGAAGTAESHGHPGVRFVPVADAPAATVYLARPAHPTHPRTDDFRAAVRD
                     VVSGGGDGGDGRASGA"
     CDS             16988..17587
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_kind="biosynthetic-additional"
                     /note="Orf6"
                     /product="hypothetical protein"
                     /protein_id="AIT38309.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 9.5e-11, bitscore:
                     31.1, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRILLVGAGGTLGGAVRAALSERGHEVVGVGRTGGDLVADVTDPE
                     AVARLYAQAGPLDAVAVAAGDAVFRPLAELTADDIAATLRGKALGQLELVRQGARHVAP
                     TGSFTLISGILTEEPIPAGAAASAANGAVEAFVRAAAIELPPQRINAISPTVAEESLPA
                     YAPFFPGMEPVPASRVATAYVRSIEGAQTGRIYRVH"
     CDS             17624..18094
                     /codon_start=1
                     /note="Orf7"
                     /product="hypothetical protein"
                     /protein_id="AIT38310.1"
                     /transl_table=11
                     /translation="MDGQQALMALIDSAWAELNGSDAGRRDLCLDTVSDVLETGRLAQD
                     DVERTVGRLATVALSGENHAVRESALHAVCTASAHYELPYRVVEPLAAGADGFEPLLLA
                     YVLSVLGSTRDRAALPVVERFLHHPHPEVRQEASEALTDLDRHREPTRDTTA"
     CDS             complement(18174..19364)
                     /codon_start=1
                     /note="Orf8"
                     /product="hypothetical protein"
                     /protein_id="AIT38311.1"
                     /transl_table=11
                     /translation="MSQDIREIVPASCALLGLGEPTHLEPAFGWVRNDLFGQLAGIGFR
                     SVALEVDRVAALAVDDYVRKGRGTLDAVMREGFSHGWGESAANRRLVAWMHAYNRNRPP
                     QERLAFHGFDAPTESMSAPSPRPYLEHARAHLGLDLGADLAELAGDDARWSRPEAVLDA
                     AASPGAEPATTALLALADDLWGALYARAPERIAATSREEWHRAGVHLAAGRGLLRYHRQ
                     AARPMDQPERVRGLLAVRDGLMAENLLAIRAAEAGRGPTLVCAHNLHLQRPPSSMRLGD
                     ADLTWSGAGAVVGALLGAEYAFVAGSLGRSAAIGLGEPEPDTYEGLLQRRAEGSGWGLT
                     AAPGAGSARTRTDTVPQQGYFPLGAAVLAGAAAVLHIPDGPRTTAGHQADAATAAG"
ORIGIN
        1 ggcgcggtgg gggccgcgcg gggtggtgcg gagagagccg agtggcgggc ggggcgccgg
       61 cgtgccgagg gttccggcgt gcagtgggtg gcgggtgggg gagcggggtc aaggcggacc
      121 gggcgcttcc ggacatcttt tgcgcggccc tgcgggacgc ggagaccgcc gggacacccg
      181 ttccgtgccc gtacgggtgt ccgtacgggg acgcccggcc ccgcgccggc gcccccgatc
      241 cgggccgaaa gtcccggatc cgagggcgaa ttggcctgcc gcggattggc cgatattcga
      301 tcgggcgggc ccccggaacc ggtccggcga cctgtgccgg gaattccgta cgctacggta
      361 acttcgctgt ccgtcaccag tgacggccct cagccattta ccgaccggag ccactgacct
      421 ttcgacggcc cggcggatcg ccatggaggg cgaatatgag taccaccgag cgccgcagcc
      481 gaatagaggc cctcggcgcc tttcttcccg cgggacgcga gaccaacgac gaactccgcg
      541 cgaaggtgcc gaacctgggt gacgcggacg tccgccggat caccggcatc gccgagcgcc
      601 gcgtccacga ccccgacccg gccgccggcg aggactcctt cgggatggcc ctcgccgcag
      661 cccgcgactg cctggccgtc tcccgccacc gggcggccga cctcgacgtc gtcatcagcg
      721 cgtccatcac ccgcgtgaag gacggcagcc gcttccactt cgagccgtcc ttcgccggaa
      781 tgctggccaa ggagctgggc gcccggcccg ccatcagctt cgacgtctcc aacgcctgcg
      841 ccggcatgat gaccggcgtg tggctgctgg accggatgat ccgctccggc gccgtccgca
      901 gcggcatggt cgtcagcggc gagcaggcca cccgcgtcgc ccgcaccgcc gcccgcgaac
      961 tgcgcgactc ctacgacccg cagttcgcct ccctctcggt cggcgactcc gccgccgcgg
     1021 tcgtcctcga cgaatccacc gaccccgccg accgcatcca ctacatcgag ctcatgacct
     1081 gcgccgcata ctcccacctg tgcctgggaa tgccgagcga ccgcagccag ggaatcggcc
     1141 tctacaccga caacaagaag atgcacgacc gggaacgcct caaactgtgg ccgcggttcc
     1201 acgaggattt cctcgccaag aacgggcgcc gtttcgagga cgaggaattc gaccacatca
     1261 ttcaacacca ggtcggcacc cggttcatcg agtacgccaa ccggaccgcc gaggccgagt
     1321 tcgccgcgcc catgccgccg tccctccagg tcgtggaaca gtacggcaac accgccacca
     1381 cctcccactt cctgaccctg cgcgaccacc tccgccgcac ccgcggcgcg ggagccaccg
     1441 gcaccggtac aggccccggg tccggccccg gcgccggccc ggcgcgggag gccgcgggcg
     1501 ccaagtacct cctggtgccc gccgcctccg gcctcgtcac cggcgcgctg tccgcaaccg
     1561 tcacccacgc aggggcctga tccgccatgg gcatcgtcat caccgcatcc gcgaccgcca
     1621 cccacaccga ccccggcacg cccgcctccg ccgtcgacct cgccgggcgc gccgcacgcc
     1681 gctgcctcgc ccacgcccgc gtatccccgt ccggcgtcgg cgtcctcgtc aacgtcggcg
     1741 tctaccgcga gaacaacacc ttcgaaccgg ccctcgccgc cctggtccag aaggaaaccg
     1801 gcatcaaccc cgactacctc gccgacccgc agcccgccgc cggcttctcc ttcgacctca
     1861 tggacggcgc ctgcggcgtc ctcagcgccg tccaggccgg ccagtccctc ctctccaccg
     1921 gcaccaccga gaggctcctc atcaccgccg ccgacgtcca ccccggcggc gacgcctccc
     1981 gcgaccccga ctacccctac gccgacctcg ccggagcgtt cctcctcgaa cgagacgccg
     2041 accccgacac cggcttcggc cccgtacgcc actacggcgg cggcgaccgg cccaccgacg
     2101 tcgccggcta cctcgacctc gacaccatgg gcagcggcgg acgctcccgc atcaccgtcc
     2161 accgcacccc cggccacgag cagcgcaccg gcgaactcgc cgcggccgcg gtcgccgcgt
     2221 acaccggcga gttcggcctc gacgccggcc gcaccctcgt catcggcccg gacgcccccg
     2281 ccggcgtcgg cgacgggccg ggaggaggac ggccgcacac cgcggccccg gtcctcggct
     2341 acctccacgc cctggaaagc gcccgccccg aaggcgtcga caccctcctg ttcgtcaccg
     2401 ccggcgccgg accgcgagca gccgtcgcct cctaccgccc gcagggatgg tgaccgccgt
     2461 ggcgaccacg accgccaccc cggcggcggc ccggcccgca gccgccgacg acctcggcgc
     2521 gcacagcctg gccggcctcc tcgaacgcaa cgcccgggcc ttccccgaca aaccggccgt
     2581 catccacccc gccgccggcc cccggcggga cggcgccagc cccgcctacc ggaccctcac
     2641 ctacggccgg ctccagcagg ccgtcgagga gctcgccgcc ggcctcaccc gcgccggcat
     2701 caccaagggc accaagaccg tcctgatggc gccgcccgga cccgaactgt tcgccctcgc
     2761 cttcgccctc ttccgggtgg gtgccgtacc cgtcgtcgtc gaccccggca tgggcgtccg
     2821 ccggatgctg cactgctacc gcaccgtcgg cgccgaggcg ttcatcggac cgcccctcgc
     2881 ccacgccgcc cgcctcctcg gccgccgcgc cttcgccggc atccgcgtcc ccgtcaccct
     2941 cggccggcac cgcctcggcc gggcccgcac cctcgccgcc gtacgcgccc tcggcgcccg
     3001 gggcggcgcg gccgcacccg tcgccgcggg ccgcgacgac ctgctgatga tcggcttcac
     3061 caccggcagc accggccccg ccaagggcgt cgagtacacc caccgcatgg cgctgtcggc
     3121 cgcccgccag atcgaggccg tgcacggccg cacccgcgac gacacctccc tcgtcaccct
     3181 gccgttctac ggggtcctcg acctcgtcta cggctccacc ctcgtcctcg ccccgctggc
     3241 cccctcccgc gtcgcccagg ccgaccccgc gctcgtcgtc gacgctctgg aacgcttccg
     3301 cgtcaccacg atgttcgcct cacccgccct cctcggcccc ctcgccgccc acctcgccgc
     3361 agccgccccc ggccgccacc cgctccccga cctgcgctgc gtcgtcggcg gcggagcacc
     3421 cgtcccggac acgacggtgg ccgcgctgcg ccgcgcactg gacccgcggg cccgcatcca
     3481 cgtcacgtac ggggcgacgg aggcgctgcc catcacgtcg atcgaggcgg aagaactcct
     3541 cggcccggag gacggaggtg agggcggggg cagtggcgtc ggcggcgccg gttccggcgg
     3601 tacggcggca cgcgccgccg aaggcgccgg aacctgtgtg ggacgccccg tccccggcat
     3661 cggcctcgcc gtcctgcccg tcaccgacgg cccgctgacc ggttccgtac cgcacctccc
     3721 gacgggccgg gtcggcgaga tcgccgtccg cggcgactgc gtcagccccc gctaccacca
     3781 ctccccggac gccgaccggc tccacaaggt ccccgacgac accgaccccg ccggccccgc
     3841 ctggcaccgc accggcgacc tcggctacct cgacgacgac ggccgcctct ggttctgcgg
     3901 ccgctccgcc caacgcgtcc gcaccgggac cggcgacctg cacacggtcc gctgcgaggg
     3961 cgtcttcaac gcccaccccc aggtgcgccg cacggccctg gtcggcatcc ccgcctcccc
     4021 ggacagcgga tggggtcgcg gcggccgcac gaccacccgc agcggcaccg ggagcggagg
     4081 caccggcacc gcgcgcggcg ccaccgagag cagcgtcgcc gcgggcaacg gaaacacgag
     4141 caccgccgcc gcccctacca cggccacgga caacggcccc gcgcacagcg cgaccccgcc
     4201 ctgcgagacg accggcaacg gaactccccg ccggcccacc ccggccaggg tctccgcggt
     4261 cagcgccccc gcgcacagcg cgaccacggt cagcggcagc tccggccgcg ccgccgcggt
     4321 cagcgggagc gcggccagcg ccgccccggg cagtgagacg gtcgtcggcg gctccgcggg
     4381 cagcacctcc gcgcccggtg cgaccactgc cggcgcgagg gccggcagcg ccgccgcggg
     4441 catggcggcg gaaggcagcg gcacagcccg cagccggacc gggggcaggg gctccgccgg
     4501 cgacggaaca gccctcggcg gctccgcgac cgccgcgcca cccggcgtcg cgccgggcgg
     4561 tgtgccggcg gacccgcgcc ggaaccgcct ccgcccggtc gtgtgcgtcg agaccgtcga
     4621 cgaggacctc gacgaggccg cctggcagcg cctcaccgcc gaactgcgca ccctggcccg
     4681 cacgcacgcg ccgaccaccg acctgcagga gttcctgcac caccccggct tccccgtcga
     4741 catccggcac aacgccaaga tcggccgcga ggagctcgcc cgctgggccg agcgccgcct
     4801 cacccctccc acccccctca caccccgtca gcgcgccgcc cggatcgtcc cgctcgccgg
     4861 gtgggcctac ctcgtcggcg gcgccgtatg ggcggcagcc ttcggcgtgc ccgaagcccg
     4921 cctgccccgc ctcctgtggt gggcggacgc cgtgctcagc accgccgggc acgccgtcca
     4981 gatcccgctg gccctgcccc gggcccgcac ggccggcata ggccgccccg ccgccgtcgg
     5041 cctgaccatg ctctacgggg cgacctggtg gcggcagctg tgaagatcct gatcaccgga
     5101 gccaccggct tcctcggcgg ccacctcgcc gacgcctgcc tgcgctccgg ccacggcgta
     5161 cgcgccctcg tccgccccgg cagcaacacc gaccggctgc gcgccctgcc cggcgtcgaa
     5221 ctcgtcaccg gcgacctcac ccgccccgac tcgctccggc gcgccgccga cggctgcgaa
     5281 gccgtcctgc acagcgccgc ccgcgtcgtc gaccacggca cccgcgccca gttcaccgaa
     5341 gccaacgtca ccggcacgct ccggctgatg gacgccgccc gcgccgccgg cgtgcgccgg
     5401 ttcgtgttcg tctccagccc gagcgcgctg atgcacctgc gggaggggga ccggctcggc
     5461 atcgacgaga ccacccccta ccccacccgc tggttcaacg actactgcgc gaccaaggcc
     5521 gtcgccgagc agcacgtcct cgccgccgac accgccggct tcaccacctg cgccctgcgg
     5581 ccccgcggca tctgggggcc ccgcgaccac gccgggttcc tgccccgcct catcggcgcc
     5641 ctgcacgcgg gacgcctccc ggacctgtcg ggcggcaaac acgtcctggt ctccctctgc
     5701 cacgtcgaca acgcggtcga cgcctgcctg cgcgccgccg tgtccgcacc ggccgaacgc
     5761 atcggcggcc gcgcctactt cgtcgcggac gccgagacga ccgacctgtg gccgttcctc
     5821 gccgacgtcg ccgcccgcct cggctgcccg ccgcccgcac cccgcatccc gctgcccgcg
     5881 ggccgggcgc tcgccgcggc ggtcgagacg gcatggcgcc tgcgcccgga cgccgccgcc
     5941 cgggcccggt cgagcccgcc actgagccgg tacatgatgg ccctgctgac ccgttcgagc
     6001 acgtacgaca cgacggcggc ccgccgcgac ctcggctaca cccccgtccg cacgcaggag
     6061 gacggcctgc gcgacctggt ccgctgggtc gcctcgcaag gcggcgtcgc ctcctggacc
     6121 gccccgcgcc cccaccccgc ccacacgcac actccggacg ccacacccca cgccccggcc
     6181 cgcgccccgc acccgccgat gcccgaacct ccggccgcgg ccacccccgc acccccgccc
     6241 aaggccgaac accgcccggc cctgccccgc ccgcgctcct cccccgaggc ggactccacc
     6301 gaacagccct tcccccaccc cgccgacgcc acggacaccc caccggtgtc cggccctgca
     6361 ccggggccgg tctccgtgcc ggcccccgac cggacgcctg cgccgtcggg ctcctcgcgg
     6421 acggccggtg atgcgccggc gtgccgtgcc ggacaggcct ccggcccggc accggcgccc
     6481 gtacgcggcc ctgccgacgc ccggtccgcc gccacggggc gcggcccccg gccggtacgc
     6541 ggttcggcag agcagcgcga acaccgcgac ccgtccctgc gcgcaagcgg gaaaccgggc
     6601 agcgacggct ccggcgcccc tgccgacacc cgcccgaacc acgacccgac tcgcgccgaa
     6661 gcagcacggc cgggagacgc ggggaggggc atggcgcccg aaggggacac cgcgcgccgc
     6721 ggatccaccg atccggccgg acctgccggc cgagaggaca cctcacgatg agcaccagca
     6781 caccgaaccc gcccggcacc tcggaaccac agcgggtgtc ccgctcggcg gccgcgtccg
     6841 acctgccgag cccgaccggc actccgggct cgcccggggc acccggcgcc tccgttcccc
     6901 gggcctcgtc ggaggcgtcc ggcccggctg ccgccgcagg ccgcccttcc ggcgcgctcc
     6961 cggcgcccga gccgtccgcc gccgtgcccg gcgcttcgac ctcaccgcag gtgccccggt
     7021 cacccgcacc cgcggcgccc ggcacggcga gccaggccgg caccccgggc ccggcggagg
     7081 atgcgggctc tcccgcggcc tccagcgctt gcaccgcgcc ggtgtcgccg ggttcgtacc
     7141 tcgcgtcagg caacacggtg tcgccgtcgc ccagcccttc agccctgccg gcgacacccg
     7201 gcacaaccgt cgcacccggt gcggcgggag ggccggagct gccggaggcg gtcggctcgg
     7261 ccaccctgcc cggcgcttcc ggcacgccgg aggcacgccg gtcatccacc gccggcggtg
     7321 gatccggacc gcacagttcg gacgccacgg ccgcggccgc gtaccggcgg cccgtgtcgc
     7381 ccaccgagag gctctacctc gccgcggggg agccgcgcgg cgccatggcc ctgcggatcg
     7441 tcgtcgaagg cgacggcctg cccgacgccg ggcagctcca cgccgccgtc gcacgggccg
     7501 cggagacgtg ccccggatcc cggcttgtcc gtcgcggccc cctgtgggcg gcggacggtc
     7561 cgccgccgca catcgtccac gcggcggccg ccgagggccc gttcgacgtg tcggacccgc
     7621 gcaccgctcg cctcctcacc ggccgatccg gcccggggga ccagcccggg tgcgagctgc
     7681 tcgtcgtgcc cggtgcgggc ggggcccgta gcacgctcgt gttcagcgcc tcgcacgccc
     7741 tgatggacgg gcacggcgcc ctcacctggg tgcgggaggt attccgcgcc ctccgaggcg
     7801 aagccgccag gcccgccgcc gacccgtcca ccgacctcgg cgtgctgcgc cgactcgccg
     7861 cggaccgggg cggccgcccc cgccgacggc ccgcgccgct ccccggacgg cgctccccgc
     7921 tcggccccgc ctcggccacc ggctctgcgt cgcagacgct gtggctgcgc cgcaccctgc
     7981 ccggccggca ccccgcgctc accgcccggc tcgcccaggc cgtcgccgac acggcacggc
     8041 tcgacacgcg cgccatggtt cccgtggacc tgcgccgcca cctcccgcac ctcgccgccg
     8101 ccaccggcaa cctgaccctg cccctcttcc tcgacctgcg ccccggccag gactgggccg
     8161 ccgcccacac ccgcctgctg accgcactcg ccgcgggcgg cgaactcgcc gccgggttcg
     8221 aatccgggcc cgtcagccgc ctgccccagc cggccgtcgc cgctctgctg cgcgccgcgc
     8281 aggccgccgc cgtacgcgcc gaccggcacc tggcctccgc cgtcgtctcc cacctcggac
     8341 ggctcgacac cggcgaactc tccggcggcg ggttcaccgc cgccacggcc tatgccctgc
     8401 ccgtgcacgc cccgctggtg ccgctgtcgt tcgccgtcgc ggagacgccc tacgcgatcg
     8461 agatcaccct cggcgtccgc gccaccgacc cgtccctccc ccaccgcgcc tccgccttcc
     8521 tggactccgt actcgccgta ctcgccgcgg acggcgccgg cccgcgtacg aacaccacac
     8581 ccgcccgaac cgccgcatcc gacccgtctg ccacgactgc cgaatccggc ccggccgatg
     8641 cgtccgcgcc ggcgtccacc cggactgccc cggctgacgt gttgaccccg tccgcctcgg
     8701 ccggcgcatc cggcgtgtgc gccccgcccg gctcggccgg cgaagccgcc ccggatgcgt
     8761 cgtccgcccc ggccgcctcg gttggcgcgt accccccggt cggtgcgtcc gccgcgtccg
     8821 acgcgtcgac ccagattgcc ccggccgacg cattgacccc ggccgcctcg gccggcgggt
     8881 tccccccgga tgcccggtcc gtcccggccg cctcggttgg cgcatatgta ccggtcgatg
     8941 cgtccgacgc gtccacctgc tccgccccgg ccggcgcgtc cgcccccacc acctccgctg
     9001 acgcatccgg cgcgtgcgtt gcgtccgcct cggctgacct gcccgccccg gttgtcctgg
     9061 gtgacacgtc cgacttggcc gaggtgtccg gcgcatccgg ccggaccgcc caggctgacg
     9121 cgtgcacccc gtccgtcccg gctgacgggt ccgccccggt caccccggcc gacgcgtccg
     9181 acgcgtccgc ccggaccgtc ccggccgacg cgaccgccgc gggcaccgtc gtcgacctct
     9241 tccgcgcaca ggccgcccgt acgcccgcgg ccgtcgccct cgacgggccc gccgggccgg
     9301 taacctacgc ggagctggac cggcgttccg acgcggtcgc cgccgagctg gtgcggcgcg
     9361 ggaccgcccg ccgcgacctc gtcggcctgg tggtggaccg caccccggag ggcgtggccg
     9421 ggctgtgggg catcctcaag gccggcgccg cctacgtccc cctcgacccg gcccaccccg
     9481 gcccgcgcat ccgcggtgtc ctcgcatcct gcggcgtccg gctgacgctg acccggcgcc
     9541 acctggcggc ccacctcgcc gagttcgtcc cgggcgcgct cctggccgtc gacgacgtac
     9601 cgacgcccgc gcagcccccg ttcgcccctg tggccaccgg acccgccccc ggcgacctgg
     9661 cgtacgtcat gcacacctcc ggctcgaccg gccaccccaa gggcgtgctg atcgagcacc
     9721 gcgggctggt cgccttcgcc cgctggatga cggacctgtg ccgcgtcggc gaaggcaccc
     9781 gcttcggatt cgcctcctcc tacgccttcg acatctcctg cttcccgctg ttcctgcccc
     9841 tgctggccgg cggaacggcc gtcctcgccc ccggcgcgcc gtcccccgcg accctccgcg
     9901 cgctcgtcgg tgaacaccgc gccgacacgc tcgccctgac gccgtcccac ctggcactcc
     9961 tcgacggcga cggcggcggc cccctcagca ccctcctgct cggcggcgag gagctgaccc
    10021 cggcggccgt ccgctccgcg tacgccgcct tcggcaccgg ctgccggctg atcaacggct
    10081 acgggcccac cgaagccacc gtcgcctgcc tcgcgcacgt cgtcgacgac acggagacgg
    10141 gcccctcgct gccgctcggc acacccggcc cggacctccg cgtcgacctg gtcgcggaag
    10201 acggcgcgtc cgtcggaacc ggcccggacg acgtcggccg cgtcggcgag atcgtcgtcc
    10261 acggcatcca ggtggcccgc ggctaccacg gttcggtgtc cgccgaaccg gacggcacgg
    10321 tcgggccgtt ctcaacgggg cccgacgggc tgcgctcgta ccgcaccggc gacctcggac
    10381 gccgcctccc cggcggcggc atcgccttcg ccgggcgggc agacggccag gtgaaggtgg
    10441 ccggccaccg catcgagccc gccgagataa ccgccgccct ggaggcccac cccggcgtcg
    10501 cccgggccgc ggtcacgacc cgccgccgcc ccggcggacg ggggctggtc ctgtgcgcat
    10561 acgcggtccc gtccaccggc acggcaacca cgggcccgac ggccgccccc gccgccggcg
    10621 ccggcggccc tgcggacccc ggcaccgcgg aatccggtcc tgcctacagc ggccctgcag
    10681 ccgcgggccc ggcggactcc ggctctgctg ccaccggcac tgcaaccacg ggcctggcga
    10741 tcaccggcac cgtggccgcc ggcggccctg cggaccccgg caccgcggac gccggctctg
    10801 cccgcaccgg ccgtgcgggc acaggccggt ttgactccgg cccggcgggt tccggctcgg
    10861 cggtcaccgg ccggtcgggt tccggctcgg cggtcaccgg tgcggtgggc tccggccctg
    10921 cggccacggg ttcggcggac ttcggccgtg cggccaccga tccggcgggc tccggcccgt
    10981 cggacccccg ctccggcccc ccggtgcccc ccgccggcgc gccttcggcg gcggagctcc
    11041 gcgcccacct ggccgggatc ctgccccggc acctggtccc ggcccacatc ctcaccgtgc
    11101 cgggcctccc gaccaccgtg agcggcaaga cggacttcga cgcgctcccg gacccgttcg
    11161 ccggaaccgc cccgctcccg gccgagccgg acgggcccgc cgcgccgcac gacacgtacg
    11221 acgcggcccc cgatccccgc agcagccgca aggcgctgcg ctcccgcgtg gcccgccact
    11281 gggcgaccgt cctcggcatc gacgccgcag ccctgaccgc cgactcggac ttccaggcgc
    11341 tcggcggcga ttcgctcgcc ctcgtcgaga tgctgagcgc cgtcagcgcc gacctgctca
    11401 ccccggacga aacccgccgc ttcaccgggg acctcaccgt cctggtccgc gacctgaccc
    11461 tcgaccgggt ctgcgaccgc ctcgccgccg tacgcgaagg aaccaccgca tgatcttcgt
    11521 cggagagggc gccctgctgc gccgggccgt ctcccacgcc gccgcccgag gccaccgcgt
    11581 cgacctggtc tgctcgcccg acccgctcga cgcagcggcc gccgcccccg gcacgccgca
    11641 ccttgccgcg cccgacgtca acgccgcggc cgccgacctg gcggcggcct gcaccgacgg
    11701 gatcgtctgg tcggtcaaca accggcagct tttccgtgcc ccggtgctgt cgctggggct
    11761 ccgcatcgtc aacgtccaca acgggctgct gccgcagcac cgcggactgc cgtccgccgc
    11821 cgtcgtgttc gcgctgctcc acggggacac cgagtacggg gccaccgtgc acgaggtcga
    11881 cgccggcatc gacaccggcc ccgtcctcgc cgagcaccgc ttcccgatcg ggcccgccga
    11941 ccgcttccac gaggtgatgc tgcgcggcgt ccgcgcctgc cagaccctct tcgagcggac
    12001 gctgcccgcc gtggccgccg gcgaacgccc ccgagccgcc ccgccggacg ccgcccggcc
    12061 ttccgggtac tacgggcgtc gggccctcgc ccgcctcggg gagcaccgcg acgccccctc
    12121 cttcgtacgc gccaccgacc tcggcccctt cgcggcgcac gcacccgaag tcgccgaggc
    12181 cctgcaccgc gtacgcccct gagcggcggc gcccgccccg cggctcgggg ccggcccgac
    12241 ggggcggtac gccgatgccc gccgcggcgg agggcgccag gcgcagcctg tggtccttct
    12301 gcgcgatgcg gacgcggcag tgcaccgccc gcacgatgac ggcctacgcg tccgcgtccg
    12361 atccgccggc ccgcggcagc tccgccccgg ccgcggcctg ggcggatccc gcccctcctg
    12421 cccggccccc cgcccggccg cgcaggcccg tcacgtcgtc gtcgtcgacg gcggcggcca
    12481 cgtcggaagc cggtcccgct gccgccccga tgcggcggaa cgccaccccg tccggcacgc
    12541 cgggcacggc ggctccccgc ctaccccgac acgcaggcga actccacatc cactcaaccg
    12601 cgttgggttc cccgcagccc cgatgcgatg atcgttccgc aggtgcgcga cccggctgcc
    12661 ggtcaacggc actggggcgc accgcggcag cggaccaaca ggagggaccc ggcgtgcgcc
    12721 gactcgtcta ctacatcgcc accacgctcg acggcttcat cgccggcccg gacggcgccg
    12781 acccgaccgg cccgaacggc ttctggccga tcgcggagga ctacctccag cacctcgtgg
    12841 ccgagtaccc ggagaccctg ccggtccagg cccggcaggc gctgtccgtc accgccgagg
    12901 gcacccggtt cgacaccgtc ctcgaagggc gtcgcacgta cgagatcggc ctcgcggccg
    12961 gcatccccga cgcctacccc cacctgcggc acatcgtgtt ctcccgcacc ctcaccgagc
    13021 tcccggcccc ggcggtcgag ctggtctccg gcgacccggt ggcgaccgtg cgcgagctga
    13081 agcagcagga cggcaaggac atctggctga tcggcggcgc cgagctggcc ggctccctct
    13141 acgccgagat cgacatgctc gtcctcaagg tcgcgccgct caccctgggc gacggcatcc
    13201 cgctcttctc ccgcaaggcc gccttcgacc cgcgcacgtg gacgctcacc gaccacacgg
    13261 tcctgaagag cggggcggcg ttcctcacct acacccgccc caccgacacc gacaccgaca
    13321 ccgacaccgc ctgacgccga ggcggcgcgg ccccacccgc gccgccgtcc ggccccacca
    13381 cctgccggtt tgccgccgcc cccgccgcgc gctttaatga atgaacggtc attcattaac
    13441 tgtggaagcg ggtgggcagg gacatggcac gaccccgcgg cgtcgaggac gcggtgatcc
    13501 tgcgggccgc cgtcgacgtg atcggccggt ccgggcccgg cggcctcacc ctggccgccg
    13561 tcgcccgcga agtcgggctc gtccccggca cgctcgtgca gcggttcggc tccaagcgcg
    13621 gcctgctcct cgcgctcgcc gagcaggccg cgcgcgaagc ggaggacctg cccgtccgcg
    13681 cccgccaagg ccacgacagc cccctcgccg ccctggaggc gctgctcgtc tcctccacgg
    13741 agcacatggc gacacccgag gcgttcgcgc accatctggg attcctgtgc atcgacctga
    13801 cggacccgga attccaccgc cacgccctcg ccgtgcaccg cgcccagcag cgcgccgtcg
    13861 aagccctcct cggcgaggcg gccggggccg gggagctccg cccccgtacg gacaccgccg
    13921 cgctcgcggc gaccgtacag gccgccgcag caggcgccgg cctcgtctgg gccctcgacc
    13981 gcagcggcag cctcgccgac cgtgtgcgcc gcgaactcgc cgccgtactc cgcgcccacc
    14041 aaccccacca cccacgcccg gaggaacgat gacgtacgcc acccccgccc ggcccctcgc
    14101 cggcaaggtc gccctcgtcg ccggcggcac ccgcggcgga ggccgcggca cggccgtcgc
    14161 cctcggcgcg gccggcgcga cggtgtacgt caccggccgc agcagccgca ccgccggccg
    14221 ctcccccctc gaccgccccg agaccgtcga ggagaccgcc gcccgggtca ccgctgccgg
    14281 cggcctcggc atccccgtac gcgtcgacca cagcgacccc gacgaagtcc gcgccctcac
    14341 cgcccgcatc ggcgccgagc agtcgggccg gctcgacatc ctcgtcaact cggtctgggg
    14401 cggcgacccg ctcaccgact gggagcaccc gctgtgggag caggacctcg acaccggcct
    14461 gcggctgctc cgccaggccg tcgagacgca catcatcacc agccgccacg ccctccccct
    14521 gatggtcgcg cagggcagtg ggctcgtcgt cgaggtcacc gacggcaaca ccgcccgcta
    14581 ccgcggctcg ttcttctacg acctcgccaa gtcggccgtc atccgcctcg ccttcgccca
    14641 ggccgccgaa ctgagggagc acggcgtcgc cgccgtcgcc gtcacccccg gcttcctgcg
    14701 ttccgaggcg gtgctcgacc acttcggcgt caccgaggac acctggcgcg acgccgtcgc
    14761 ccgggatccc gacttcgcgc actccgaaag ccccgcctac ctgggccgcg cgatcgccgc
    14821 cctggccgcc gacccggacg tcctgcggtg gacgggccgc gccctcgcaa cctggtccct
    14881 gtacaaggag tacggcttca ccgacaccga cggcacccag cccgacttcg ccgcccactg
    14941 ggccgatgcc ctcgaaccgg tccacggccc cctcggcgag ccgctgtagc cgccgcctgc
    15001 cccgggtgtg ccgtccccgg ggaccccggt gcctcggcgt gcgccgggca ccagcatgct
    15061 ggtgcccggc ggggccaccg ccgggcgggc ggcgcagcgc ccgtacgcgg acgaacgacg
    15121 accggagaaa ccaggatgca ggacgcagac cgctggctga agcgcttcac ccccgcaagc
    15181 agccccgcaa ccggccgcac gcccggcacg gctgccctca cgcccctgac ccgcatccgc
    15241 tcccgcgtcg tctgcctgcc ccacgccggc ggcgccgccc ccgcctacca gccgtgggcc
    15301 cgtcacctcc ccgccgacac cgagctgttg gccgtccagt accccggccg ccaggagcgg
    15361 ctcggcgagc cctgtgccga caccctcggc gagctcgccg acgcggtcgc cgccgccctc
    15421 gccgcgctcc ccgtcctgcc caccgtcgtc ttcggccaca gcatgggctc gctcgtcgcg
    15481 tacgagaccg tcctgcggct cgcccggcac gccccgcagg cgctgcccgt gcggctcttc
    15541 gtctccggcg cgcccgcccc cgcgcacgac cgcaccaacc tgcccgcccc ggacgacgcc
    15601 tccctcctcg cgtacgtccg caaccagggc gccgcccagc ccgaggtgta cgacatcccc
    15661 gagctccgcg agctcctgat gccctccctc cgcggcgact tcgcgctgct cgcccgctac
    15721 cgcccggccc ggccgccggc ccgcgtcccc gtaccggtga cggccctcgg cggcgactcc
    15781 gacgcgggct gcccgcccga atccctcgcc acctgggccg acgccaccac cgcggacttc
    15841 gcctcccgcg tcttccccgg cggccacttc tacctccagg gccgcgagga caccctggcc
    15901 ggtctggtca cgacggccct ccgcgcggcg gcagcccgct gacgggcccg cggcggccgc
    15961 cggctcaggc gccggacgcg cggccgtcgc cgccgtcccc gccgccgctc accacgtcgc
    16021 ggaccgccgc ccggaagtcg tccgtccgcg ggtgcgtggg gtgcgcgggc cgcgccaggt
    16081 acacggtggc cgccggagcg tccgccaccg gcacgaagcg gacgcccggg tggccgtggc
    16141 tctccgccgt gcccgccgcg ccgacgccga acgccccgcc gatcgcgatc aggttcagcc
    16201 actcgtccac gttgcggacc tccaccgtcc gggccggccg ccgccccgcc ggccacagct
    16261 ccggcgacgc cgtgcccgtc tccggccaca gggccagcgg cagatcccgg cccgccgtca
    16321 gctccgccag cagcacctga tccctgcccg ccagcggatc gtcgtccggc agcgcggcga
    16381 cccggccctc cgtgtacagc gcctccgtgc ggatccgccg gtccgccggc accgtgcgca
    16441 gcaccgccag gtccgcgtcc cccgtggcca ggcccgcggt actgctgtca tgccgcacca
    16501 cctccagcgg gacgtgcggg tgcgtacgcc gccacgagcg cagcagcggc accgtctgct
    16561 gcccgagcac cgcgcacgtg tagcccaggc gcagcggccg cgacacctcc cgcgtgtcgg
    16621 cgagggccgc gtccagatgc gccaggatgg cccgggcgtg cccgagcagc gtcgtgcccg
    16681 cggcggtcag ggccggccgc cgcgaggacc ggtcgacgag ccgcaccccg acccgcgcct
    16741 ccagctgcgc cagcgtccgc gacagcgccg gctgggtgat gtgcagccgc gccgcggccg
    16801 cggtgaccgt gccagccgcg gcgaccgcgt ccagggccct caggtgccgc agctccacgc
    16861 catccatgcc tgtgaagcat agtcgctgcc cagacggcat ttccgcccgc gcggaaggct
    16921 ccgtagcgtc gaaggggccg aggcgccccg ccccggcgga gaaacgttct cagagagacg
    16981 gtgcatggtg aggatcctcc tggtcggagc gggcggcacg ctcggcggcg cggtacgcgc
    17041 ggcgctgtcg gaacgcggac acgaggtggt cggcgtcggc cgcaccggcg gcgacctggt
    17101 cgccgacgtc accgacccgg aggccgtcgc ccgcctgtac gcgcaggccg gcccgctgga
    17161 cgcggtcgcg gtcgccgcgg gcgacgccgt cttccgcccg ctcgccgaac tgaccgccga
    17221 cgacatcgcg gcgaccctgc gcggcaaggc gctcggccag ctggaactcg tccgccaagg
    17281 cgcccggcac gtggcgccga ccggctcgtt cacgctgatc agcggcatcc tcaccgagga
    17341 gccgatcccc gccggagcgg ccgcctccgc ggcgaacggc gccgtcgagg ccttcgtccg
    17401 cgccgccgcg atcgaactcc cgccccagcg catcaacgcg atcagcccca ccgtcgccga
    17461 ggagtccctc cccgcgtacg ccccgttctt ccccggaatg gaaccggtcc ccgcctcccg
    17521 cgtggccacg gcatacgtcc gctcgatcga gggcgcccag acgggccgga tctaccgcgt
    17581 ccactgacgc cggaacccgg cggcggtctc ctatgatccg gccgtggacg gacaacaggc
    17641 actgatggca ttgatcgatt cagcctgggc ggagctgaac ggctccgacg cgggccgccg
    17701 ggacctctgc ctcgataccg tcagcgacgt cctggagacg ggccgtctgg cgcaggacga
    17761 cgtcgagcgg accgtcgggc gcctcgccac cgtcgcgctc tccggcgaga accacgccgt
    17821 acgggagtcc gcgctgcacg cggtctgcac ggcctccgcg cactacgagc ttccgtaccg
    17881 ggtggtggag ccgctcgccg ccggggcgga cggcttcgaa cccctgctcc tcgcctacgt
    17941 tctgtccgtc ctcggctcca ctcgtgaccg ggcagcgctg ccggtcgtcg agcggttcct
    18001 ccaccacccc caccccgagg tccgccagga ggcctcggaa gcgctgacgg acctggaccg
    18061 gcaccgggag ccgacccggg acacgacggc gtagccccgc cgcgcgggcc gtcggccgcc
    18121 ccgcgaccca cccgggccgc gagcgtcagc cgccggcgcg gcccgggacc cggctacccg
    18181 gccgccgtcg ccgcgtcggc ctggtggccg gcggtcgtcc gcgggccgtc cgggatgtgg
    18241 aggaccgctg ccgcgcccgc caagaccgcc gccccgaggg ggaaatagcc ctgctgcggc
    18301 acggtgtcgg tgcgcgtacg ggccgagccg gccccggggg ccgcggtcag cccccaccct
    18361 gagccctccg cccgccgctg cagcagcccc tcgtacgtgt ccggctccgg ctcgcccagg
    18421 ccgatcgccg cgctgcggcc caggctgccg gcgacgaacg cgtactccgc gcccagcagg
    18481 gcccccacca ccgcgccggc accggaccag gtgaggtccg cgtcccccag ccgcatgctg
    18541 ctcggcggcc gctggaggtg gaggttgtgc gcgcagacca gcgtcgggcc ccgcccggcc
    18601 tcggcggcgc ggatggcgag caggttctcc gccatcagcc cgtcccgcac ggcgagcagc
    18661 ccccgcaccc gctccggctg gtccatgggc cgcgccgcct gccggtggta gcgcaacaga
    18721 ccgcgccccg cggcgaggtg gacgcccgcc cggtgccact cctcccgtga cgtggccgcg
    18781 atccgctccg gcgcgcgcgc gtacagtgcg ccccacaggt cgtcggcgag cgccagcagc
    18841 gccgtggtcg ccggctcggc ccccggcgag gccgccgcgt ccaggaccgc ctccgggcgg
    18901 ctccaccgcg cgtcgtcgcc ggccagctcc gcgaggtccg cgccgaggtc caggcccagg
    18961 tgggcgcgcg cgtgctccag gtacgggcgg ggactgggcg cgctcatgga ctccgtcggc
    19021 gcgtcgaagc cgtggaaggc gagccgctcc tgcgggggcc ggttccggtt gtacgcgtgc
    19081 atccacgcga cgagccgccg gttggccgcc gactcccccc agccgtggct gaacccctcg
    19141 cgcatcaccg cgtcgagggt cccccgcccc ttgcggacgt agtcgtcgac ggcgagcgcg
    19201 gccactcggt cgacctccag ggcgaccgac cggaagccga tcccggcgag ctgcccgaac
    19261 aggtcgttgc ggacccaccc gaaggcgggt tcaaggtgcg tcggctctcc caggcccagc
    19321 agggcgcacg acgcagggac gatttcccgg atgtcctgac tcatcatgtc ccgcaagcgt
    19381 atctttgaaa gcccggtgaa
//
