Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000389.1:
Cluster 1NRP124248

BGC0000389, massetolide A biosynthetic gene cluster from Pseudomonas fluorescens. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: massetolide A
ChEMBL ID: 500573
SMILES string: Copy to clipboard
Molecular formula: C55H97N9O16
Average molecular mass: 1140.4 Da
Molecular activity: Antifungal, Surfactant
Molecular target: membrane

Class-specific details

Biosynthetic class(es):
NRP
Cyclic depsipeptide (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Unknown

Nonribosomal peptide synthetases:
massA (ABH06367)
Module A1
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module A2
A specificity: Glutamate
Evidence for specificity: Structure-based inference
C domain subtype: Dual
massB (ABH06368)
Module B1
A specificity: Threonine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module B2
A specificity: Isoleucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module B3
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module B4
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
massC (ABH06369)
Module B5
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module B6
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module B7
A specificity: Isoleucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual

Gene cluster description

massetolide A (BGC0000389). Gene Cluster 1. Biosynthetic class = NRP. GenBank EU199081, positions 11695-35942. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Enzymatic assays, Heterologous expression, Proven expression in natural host, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Prof. Dr. Jos Raaijmakers (NIOO-KNAW)

Literature references

1. de Bruijn I et al. (2008) Massetolide A biosynthesis in Pseudomonas fluorescens. J Bacteriol 190(8):2777-89. doi: 10.1128/JB.01563-07. Epub 2007 Nov 9.
2. de Bruijn I et al. (2007) Genome-based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics in Pseudomonas species. Mol Microbiol 63(2):417-28. doi: 10.1111/j.1365-2958.2006.05525.x.
3. Loper JE et al. (2012) Comparative genomics of plant-associated Pseudomonas spp.: insights into diversity and inheritance of traits involved in multitrophic interactions. PLoS Genet 8(7):e1002784. doi: 10.1371/journal.pgen.1002784. Epub 2012
4. de Bruijn I, Raaijmakers JM. (2009) Diversity and functional analysis of LuxR-type transcriptional regulators of cyclic lipopeptide biosynthesis in Pseudomonas fluorescens. Appl Environ Microbiol 75(14):4753-61. doi: 10.1128/AEM.00575-09. Epub
5. De Souza JT et al. (2003) Biochemical, genetic, and zoosporicidal properties of cyclic lipopeptide surfactants produced by Pseudomonas fluorescens. Appl Environ Microbiol 69(12):7161-72.