LOCUS       BGC0000391             14231 bp    DNA     linear   BCT 04-AUG-2012
DEFINITION  Marinactinospora thermotolerans strain SCSIO 00652
            methylpendolmycin/pendolmycin gene cluster, complete sequence.
ACCESSION   BGC0000391
VERSION     BGC0000391
KEYWORDS    .
SOURCE      Marinactinospora thermotolerans
  ORGANISM  Marinactinospora thermotolerans
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Nocardiopsaceae; Marinactinospora.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:31:51
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..732
                     /codon_start=1
                     /experiment="gene disruption"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PIG-L"
                     /gene_kind="biosynthetic-additional"
                     /note="MpnA"
                     /product="N-acetylglucosaminylphosphatidylinositol
                     deacetylase"
                     /protein_id="AFO85452.1"
                     /sec_met_domain="PIG-L (E-value: 1.1e-27, bitscore: 86.2,
                     seeds: 137, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTSDDIPPLPEDWQRALAVVAHPDDLEFGASAAITRWVGQGRQV
                     AELLVTRGEAGIDTIAPQECGPLRTAEQLAAARVVGVEEVAFLDFPDGSLQYGLDLRRE
                     LARAIRRHRPEVLISLNFRETFPGSAAFNHADHRALGPALLDAVRDAANRWMFPDLSDE
                     GLRPWSGVRFAAFSGSPRATHHVPLSEGELAAGIASLAAHRAYLSALGGFDHEAFLTAE
                     ARSAGESCGTGLATLFEVIPT"
     source          1..14231
                     /db_xref="taxon:531310"
                     /mol_type="genomic DNA"
                     /organism="Marinactinospora thermotolerans"
                     /strain="SCSIO 00652"
                     /type_material="type strain of Marinactinospora
                     thermotolerans"
     misc_feature    1..14231
                     /note="methylpendolmycin/pendolmycin gene cluster"
     protocluster    1..14231
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1145:9767]"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1146..9767
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     cand_cluster    1..14231
                     /SMILES="NC([*])C(=O)NC(CC1=CNc2c1cccc2)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX))"
                     /detection_rules="(indsynth or dmat or indole_PTase)"
                     /kind="neighbouring"
                     /product="NRPS"
                     /product="indole"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..14231
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000391"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14231
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /contig_edge="True"
                     /product="NRPS"
                     /product="indole"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX))"
                     /rules="(indsynth or dmat or indole_PTase)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             1146..9767
                     /NRPS_PKS="Domain: Condensation_Starter (19-183). E-value:
                     6.6e-29. Score: 93.0. Matches aSDomain:
                     nrpspksdomains_AFO85453.1_Condensation_Starter.1"
                     /NRPS_PKS="Domain: AMP-binding (422-815). E-value:
                     7.4e-103. Score: 336.1. Matches aSDomain:
                     nrpspksdomains_AFO85453.1_AMP-binding.1"
                     /NRPS_PKS="Domain: nMT (903-1121). E-value: 6.5e-112.
                     Score: 365.8. Matches aSDomain:
                     nrpspksdomains_AFO85453.1_nMT.1"
                     /NRPS_PKS="Domain: PCP (1333-1402). E-value: 3.3e-25.
                     Score: 80.0. Matches aSDomain:
                     nrpspksdomains_AFO85453.1_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1414-1711). E-value:
                     1.6e-110. Score: 360.7. Matches aSDomain:
                     nrpspksdomains_AFO85453.1_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1881-2276). E-value:
                     8.9e-104. Score: 339.1. Matches aSDomain:
                     nrpspksdomains_AFO85453.1_AMP-binding.2"
                     /NRPS_PKS="Domain: PCP (2382-2449). E-value: 1.2e-21.
                     Score: 68.6. Matches aSDomain:
                     nrpspksdomains_AFO85453.1_PCP.2"
                     /NRPS_PKS="Domain: TD (2490-2735). E-value: 2.7e-82. Score:
                     267.5. Matches aSDomain: nrpspksdomains_AFO85453.1_TD.1"
                     /codon_start=1
                     /experiment="gene disruption"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127:condensation domain-containing protein (Score:
                     384.9; E-value: 1e-116)"
                     /gene_kind="biosynthetic"
                     /note="MpnB"
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="AFO85453.1"
                     /sec_met_domain="PP-binding (E-value: 1.6e-15, bitscore:
                     46.4, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 4.2e-70, bitscore:
                     225.0, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.4e-112, bitscore:
                     365.1, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 6.1e-68, bitscore:
                     217.5, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFHRSTGSIFVCNSYGPWSPLTSAQNAVLESWRAARHAPQVCAVG
                     EYVDIRGPLDPTLFTTAFRRAAEETDALRLGFAEIDGEIRQRLTTSPTPAFRRIDLTHL
                     ADPWEEVHGLLARERLLPRLPAAPHEVYDHALVRLAPDRLLWVHHAHPLALDRTGLAAL
                     RSRVAAYYTDLAGGPPADATRPSRSPDTDRPIGARPPRGHSGALPAGLRDRLGTFAADP
                     GAAVLDPFLAAAALHGRDGHDEAVVGLVTGEPDQRGRAAPSHARRHALRLAADPHVSAA
                     ELLRAASLLRRDAATRPVDPPRDGEETARKPPRVVVETDASVVFLGPHRTTVHTLDPGN
                     GDHTAVIVREDTVELFIGAGADQAADPTLQHRRLLELVAQVLATPETGTAQARATAPAE
                     QPGRPPEATGAPSSEPWACLPDLFLRQARRDPDRVAIVDGDEQMSYAELDARSNRLAHR
                     LVHRGCSPGASVGLALPRSADLLVATLAVLKAGAAYVPLDLRSPGPRLRRILQETGTRI
                     VITEPGTHGHDAFAGMALLPVGPDPAGADPGTPRSALHPDSLAYVMYTSGSTGAPKGIA
                     ISHRAVAALAADRRWDGGAHERVLLHSPHAFDASTYEIWVPLLRGGTVVVAPPGETDPV
                     RLRRLLRAERIGALFVTTALFSLVAEELPDCFATVREVWTGGERMPPKAVERVLDHCPR
                     LHLVHVYGPTESTTFATCHPVPRAEVGDPVPIGGPMDNTRCHVLDDQLRPVPPGVPGEL
                     YLAGIGLARGYTGAAGLTAERFVAEPSGPPGSRMYRTGDIVRWRADGALEFLHRADGQV
                     KIRGFRIEPGEVEKALCAHPGVSAAAVVAREERPGERRLVGYIVPADRDEGAADGEGDQ
                     IGEWRGIYDRLYTAPAETPLGTDFTGWNSSYDGTAIPEEQMREWRDATVERIRALRPRR
                     VLEIGVGTGLLMAGLAPHCESYWGTDFSAPAIEGLRRGIARDPALARRVELRVCAADEL
                     GGLPAGFFDTVVINSVTQYFPNNGYLTRVLAGAAGLLAPGGAIFVGDVRDLRLARPFHT
                     EVALRRAAPGDDAAAVRATIERDSAAEQELMVSPDYFMALCSHVPRLAGADIQVKGGRH
                     RNEMTQYRYDVVLRADRPTTPEEDVDVRTLVWGRDLAGLDELAGLLAARTHRHVRLLDV
                     PNARVAPAALAWRALSEERDADLARRRLDEAGRGLPEPAELEEVGRLLGLRTALTWAAD
                     ASDGALDAHFLALGAEEPVPCGLYRSRGTADAPLRAHTNSPRTSREAGALVASVRDLLG
                     ERLPDYMVPHHIVVVDRLPLTRNGKLDRAALPAPKPGTTLDGRAPRTPTEERLCALYTE
                     VLGVSPVSIDDDFFELGGHSLSATRLVARIRAGLEVEVPLRAVFETPRVVDLVERLGRT
                     AARPALTHRARPERVPLSPGQARLWFLHRFSGADPLYNVPFRIRLSGPLDHAALHAALC
                     DLVDRHESLRTVFPEHEGVPYQLVTEAQVRLPVVASTPQRVEADARAAARCGFDIATEI
                     PFRAVLFRLADDAHVLLIVVHHIACDGWSLGPLWRDLATAYRERLAGRRPFDRPLPVQY
                     ADYTLWQRDLLDEGNPEGTAETQLRHWREGLAGLPDRIVLPTDRPRPPLARHSGATVRF
                     SVPAALHDRLVSVAQEHGATVFMALHAGLAALLCRLGGGSDIPVGSPIAGRTDPALDDL
                     VGFFVNTLVIRTDTSGDPSFAELLGRVREVVLAAHEHQDLPFERLVEELRPARSLAHHP
                     LFQVMLALQNAPGSDFRLAGLTAEADLMDIGTSRFDLVLSLTERFADDGTPQGMDGIAE
                     FDTDLFDPGSVDALVRRLLRLLDRVTARPGLRIGEVDLLEGTEHRRLLREWNDSGGRTA
                     PATFPELFQRQVTAAPEATALVHGEDTWSYRALNARANRFARALIRYGAGPETHVAVVL
                     PRSAELVTVLVAIMKAGAAYLPFEAAQPARRLEAMLAEARPALVIGTADTLAELPGDSP
                     VLPLDRLEAEAALLADNDVTDGERGTPLLPDHPAYLIYTSGSTGRPKGVVVTHSGFSGM
                     ADIQRERFGVTRDSRVLQFAPISFDGAVWEIFGTLAVGATLVTAPADQVMPGPDLVRLL
                     RARGITHLTLPPTALAVMEPADLDGVSSLIVSGEASSGALVAAWSPGRRMLNGYGPTET
                     TVCATLSDPLAGAAPPPIGRPAIGARCYVLDDRLRPVPPGSPGELYVAGAGLARGYLGD
                     PGRSAARFVADPFGVPGERMYRTGDLARWTRGGVLEFLGRRDEQVKIRGFRIEPGEVAA
                     ALERHPSVGRAAVVAREDRPGDRRLVAYVVSTGEPVDAAEVRRHAADLLPAHLVPSVVV
                     PIAALPTLPSGKVDRAALPAPVAVTVSRGRRPSSPLEHTLCGLFEELLGVPGITVDDGF
                     FDLGGHSLLVPRLLRGVRDRTGADLSVAQLFETPTVAGLAHEIGGGRGGRARPSEPDPA
                     ADTVLDPLVKVPADGIDLARNAAPRHVLLTGATGFLGAALLDQLLRHTDATVHCLVRAL
                     DRDAGLARIRDNLHRHGVELDPARAARVVAVPGDLARPRLGLSEERFHALAELLDAVYH
                     NGAGVSAVEPYSRLRAGNVLGTHEVIRLATRVRAVPVHFVSTAAVAVSSDDGDGPFLEG
                     QRAPSASLLPSGYVATKWAAEGLVRAAGERGLPVTIHRPGRISGYSRTGVGGSGDTLWQ
                     LVRAMLLLGAVPRSAEHDAAMVDMVPVDHVAGAIVHLARRPEAMGRVHHLTCPRPIPFP
                     RLLALFRDRGYVLAPLADEEWAELLLRRAEDPDTGTGLAAAALLVEALPGIVRLGALRF
                     DRRNTLRGLAEAGWDLSGAEPVLLERYIDHLVRSGVFPAPARTPQQPPTSERAEV"
     protocluster    1167..14231
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[11166:12291]"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      11167..12291
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="2"
     cand_cluster    1167..14231
                     /candidate_cluster_number="2"
                     /contig_edge="True"
                     /detection_rules="(indsynth or dmat or indole_PTase)"
                     /kind="single"
                     /product="indole"
                     /protoclusters="2"
                     /tool="antismash"
     aSDomain        1203..1694
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AFO85453.1_Condensation_Starter.
                     1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="6.60E-29"
                     /label="AFO85453.1_Condensation_Starter.1"
                     /locus_tag="AFO85453.1"
                     /protein_end="183"
                     /protein_start="19"
                     /score="93.0"
                     /tool="antismash"
                     /translation="PLTSAQNAVLESWRAARHAPQVCAVGEYVDIRGPLDPTLFTTAFR
                     RAAEETDALRLGFAEIDGEIRQRLTTSPTPAFRRIDLTHLADPWEEVHGLLARERLLPR
                     LPAAPHEVYDHALVRLAPDRLLWVHHAHPLALDRTGLAALRSRVAAYYTDLAGGPPADA
                     T"
     aSModule        1203..5351
                     /complete
                     /domains="nrpspksdomains_AFO85453.1_Condensation_Starter.1"
                     /domains="nrpspksdomains_AFO85453.1_AMP-binding.1"
                     /domains="nrpspksdomains_AFO85453.1_nMT.1"
                     /domains="nrpspksdomains_AFO85453.1_PCP.1"
                     /locus_tags="AFO85453.1"
                     /monomer_pairings="X -> NMe-X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1269..1370
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0001"
                     /evalue="2.80E-13"
                     /label="C2_starter_024-063"
                     /locus_tag="AFO85453.1"
                     /protein_end="75"
                     /protein_start="41"
                     /score="42.4"
                     /tool="antismash"
                     /translation="CAVGEYVDIRGPLDPTLFTTAFRRAAEETDALRL"
     CDS_motif       1602..1658
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0002"
                     /evalue="8.30E+00"
                     /label="C3_starter_134-155"
                     /locus_tag="AFO85453.1"
                     /protein_end="171"
                     /protein_start="152"
                     /score="-0.2"
                     /tool="antismash"
                     /translation="LALDRTGLAALRSRVAAYY"
     aSDomain        2412..3590
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AFO85453.1_AMP-binding.1"
                     /evalue="7.40E-103"
                     /label="AFO85453.1_AMP-binding.1"
                     /locus_tag="AFO85453.1"
                     /protein_end="815"
                     /protein_start="422"
                     /score="336.1"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FLRQARRDPDRVAIVDGDEQMSYAELDARSNRLAHRLVHRGCSPG
                     ASVGLALPRSADLLVATLAVLKAGAAYVPLDLRSPGPRLRRILQETGTRIVITEPGTHG
                     HDAFAGMALLPVGPDPAGADPGTPRSALHPDSLAYVMYTSGSTGAPKGIAISHRAVAAL
                     AADRRWDGGAHERVLLHSPHAFDASTYEIWVPLLRGGTVVVAPPGETDPVRLRRLLRAE
                     RIGALFVTTALFSLVAEELPDCFATVREVWTGGERMPPKAVERVLDHCPRLHLVHVYGP
                     TESTTFATCHPVPRAEVGDPVPIGGPMDNTRCHVLDDQLRPVPPGVPGELYLAGIGLAR
                     GYTGAAGLTAERFVAEPSGPPGSRMYRTGDIVRWRADGALEFLHRADGQVKIR"
     CDS_motif       2598..2639
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0003"
                     /evalue="5.00E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="AFO85453.1"
                     /protein_end="498"
                     /protein_start="484"
                     /score="19.5"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPLD"
     CDS_motif       2823..2882
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0004"
                     /evalue="3.80E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="AFO85453.1"
                     /protein_end="579"
                     /protein_start="559"
                     /score="28.6"
                     /tool="antismash"
                     /translation="AYVMYTSGSTGAPKGIAISH"
     CDS_motif       3246..3269
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0005"
                     /evalue="7.40E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="AFO85453.1"
                     /protein_end="708"
                     /protein_start="700"
                     /score="3.5"
                     /tool="antismash"
                     /translation="YGPTESTT"
     CDS_motif       3381..3470
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0006"
                     /evalue="7.00E-13"
                     /label="NRPS-A_a6"
                     /locus_tag="AFO85453.1"
                     /protein_end="775"
                     /protein_start="745"
                     /score="40.7"
                     /tool="antismash"
                     /translation="PPGVPGELYLAGIGLARGYTGAAGLTAERF"
     CDS_motif       3555..3620
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0007"
                     /evalue="2.00E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="AFO85453.1"
                     /protein_end="825"
                     /protein_start="803"
                     /score="32.7"
                     /tool="antismash"
                     /translation="FLHRADGQVKIRGFRIEPGEVE"
     CDS_motif       3660..3713
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0008"
                     /evalue="1.70E+00"
                     /label="C5_LCL_267-296"
                     /locus_tag="AFO85453.1"
                     /protein_end="856"
                     /protein_start="838"
                     /score="1.3"
                     /tool="antismash"
                     /translation="VVAREERPGERRLVGYIV"
     aSDomain        3855..4508
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AFO85453.1_nMT.1"
                     /domain_subtypes="nMT"
                     /evalue="6.50E-112"
                     /label="AFO85453.1_nMT.1"
                     /locus_tag="AFO85453.1"
                     /protein_end="1121"
                     /protein_start="903"
                     /score="365.8"
                     /tool="antismash"
                     /translation="GTAIPEEQMREWRDATVERIRALRPRRVLEIGVGTGLLMAGLAPH
                     CESYWGTDFSAPAIEGLRRGIARDPALARRVELRVCAADELGGLPAGFFDTVVINSVTQ
                     YFPNNGYLTRVLAGAAGLLAPGGAIFVGDVRDLRLARPFHTEVALRRAAPGDDAAAVRA
                     TIERDSAAEQELMVSPDYFMALCSHVPRLAGADIQVKGGRHRNEMTQYRYDVVLR"
     misc_feature    4503..4505
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     aSDomain        5145..5351
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AFO85453.1_PCP.1"
                     /evalue="3.30E-25"
                     /label="AFO85453.1_PCP.1"
                     /locus_tag="AFO85453.1"
                     /protein_end="1402"
                     /protein_start="1333"
                     /score="80.0"
                     /tool="antismash"
                     /translation="EERLCALYTEVLGVSPVSIDDDFFELGGHSLSATRLVARIRAGLE
                     VEVPLRAVFETPRVVDLVERLGRT"
     aSDomain        5388..6278
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AFO85453.1_Condensation_LCL.1"
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.60E-110"
                     /label="AFO85453.1_Condensation_LCL.1"
                     /locus_tag="AFO85453.1"
                     /protein_end="1711"
                     /protein_start="1414"
                     /score="360.7"
                     /tool="antismash"
                     /translation="ERVPLSPGQARLWFLHRFSGADPLYNVPFRIRLSGPLDHAALHAA
                     LCDLVDRHESLRTVFPEHEGVPYQLVTEAQVRLPVVASTPQRVEADARAAARCGFDIAT
                     EIPFRAVLFRLADDAHVLLIVVHHIACDGWSLGPLWRDLATAYRERLAGRRPFDRPLPV
                     QYADYTLWQRDLLDEGNPEGTAETQLRHWREGLAGLPDRIVLPTDRPRPPLARHSGATV
                     RFSVPAALHDRLVSVAQEHGATVFMALHAGLAALLCRLGGGSDIPVGSPIAGRTDPALD
                     DLVGFFVNTLVIRTDT"
     aSModule        5388..9350
                     /complete
                     /domains="nrpspksdomains_AFO85453.1_Condensation_LCL.1"
                     /domains="nrpspksdomains_AFO85453.1_AMP-binding.2"
                     /domains="nrpspksdomains_AFO85453.1_PCP.2"
                     /domains="nrpspksdomains_AFO85453.1_TD.1"
                     /final_module
                     /locus_tags="AFO85453.1"
                     /monomer_pairings="Trp -> Trp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       5454..5570
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0009"
                     /evalue="1.50E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="AFO85453.1"
                     /protein_end="1475"
                     /protein_start="1436"
                     /score="59.2"
                     /tool="antismash"
                     /translation="PLYNVPFRIRLSGPLDHAALHAALCDLVDRHESLRTVFP"
     CDS_motif       5763..5828
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0010"
                     /evalue="1.40E-11"
                     /label="C3_LCL_132-143"
                     /locus_tag="AFO85453.1"
                     /protein_end="1561"
                     /protein_start="1539"
                     /score="36.7"
                     /tool="antismash"
                     /translation="VHHIACDGWSLGPLWRDLATAY"
     CDS_motif       5877..5915
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0011"
                     /evalue="1.00E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="AFO85453.1"
                     /protein_end="1590"
                     /protein_start="1577"
                     /score="14.9"
                     /tool="antismash"
                     /translation="QYADYTLWQRDLL"
     CDS_motif       6180..6269
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0012"
                     /evalue="2.10E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="AFO85453.1"
                     /protein_end="1708"
                     /protein_start="1678"
                     /score="49.0"
                     /tool="antismash"
                     /translation="DIPVGSPIAGRTDPALDDLVGFFVNTLVIR"
     CDS_motif       6318..6437
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0013"
                     /evalue="1.40E-22"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="AFO85453.1"
                     /protein_end="1764"
                     /protein_start="1724"
                     /score="72.2"
                     /tool="antismash"
                     /translation="REVVLAAHEHQDLPFERLVEELRPARSLAHHPLFQVMLAL"
     aSDomain        6789..7973
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AFO85453.1_AMP-binding.2"
                     /evalue="8.90E-104"
                     /label="AFO85453.1_AMP-binding.2"
                     /locus_tag="AFO85453.1"
                     /protein_end="2276"
                     /protein_start="1881"
                     /score="339.1"
                     /specificity="substrate consensus: Trp"
                     /tool="antismash"
                     /translation="FQRQVTAAPEATALVHGEDTWSYRALNARANRFARALIRYGAGPE
                     THVAVVLPRSAELVTVLVAIMKAGAAYLPFEAAQPARRLEAMLAEARPALVIGTADTLA
                     ELPGDSPVLPLDRLEAEAALLADNDVTDGERGTPLLPDHPAYLIYTSGSTGRPKGVVVT
                     HSGFSGMADIQRERFGVTRDSRVLQFAPISFDGAVWEIFGTLAVGATLVTAPADQVMPG
                     PDLVRLLRARGITHLTLPPTALAVMEPADLDGVSSLIVSGEASSGALVAAWSPGRRMLN
                     GYGPTETTVCATLSDPLAGAAPPPIGRPAIGARCYVLDDRLRPVPPGSPGELYVAGAGL
                     ARGYLGDPGRSAARFVADPFGVPGERMYRTGDLARWTRGGVLEFLGRRDEQVKIR"
     CDS_motif       6978..7013
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0014"
                     /evalue="1.10E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="AFO85453.1"
                     /protein_end="1956"
                     /protein_start="1944"
                     /score="12.5"
                     /tool="antismash"
                     /translation="AIMKAGAAYLPF"
     CDS_motif       7221..7280
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0015"
                     /evalue="2.20E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="AFO85453.1"
                     /protein_end="2045"
                     /protein_start="2025"
                     /score="32.3"
                     /tool="antismash"
                     /translation="AYLIYTSGSTGRPKGVVVTH"
     CDS_motif       7635..7661
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0016"
                     /evalue="2.30E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="AFO85453.1"
                     /protein_end="2172"
                     /protein_start="2163"
                     /score="7.7"
                     /tool="antismash"
                     /translation="YGPTETTVC"
     CDS_motif       7764..7883
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0017"
                     /evalue="1.70E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="AFO85453.1"
                     /protein_end="2246"
                     /protein_start="2206"
                     /score="45.9"
                     /tool="antismash"
                     /translation="PPGSPGELYVAGAGLARGYLGDPGRSAARFVADPFGVPGE"
     CDS_motif       7938..8000
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0018"
                     /evalue="5.70E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="AFO85453.1"
                     /protein_end="2285"
                     /protein_start="2264"
                     /score="34.4"
                     /tool="antismash"
                     /translation="FLGRRDEQVKIRGFRIEPGEV"
     CDS_motif       8046..8102
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85453.1_0019"
                     /evalue="2.40E+01"
                     /label="C5_LCL_267-296"
                     /locus_tag="AFO85453.1"
                     /protein_end="2319"
                     /protein_start="2300"
                     /score="-2.4"
                     /tool="antismash"
                     /translation="VAREDRPGDRRLVAYVVST"
     aSDomain        8292..8492
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AFO85453.1_PCP.2"
                     /evalue="1.20E-21"
                     /label="AFO85453.1_PCP.2"
                     /locus_tag="AFO85453.1"
                     /protein_end="2449"
                     /protein_start="2382"
                     /score="68.6"
                     /tool="antismash"
                     /translation="EHTLCGLFEELLGVPGITVDDGFFDLGGHSLLVPRLLRGVRDRTG
                     ADLSVAQLFETPTVAGLAHEIG"
     aSDomain        8616..9350
                     /aSDomain="TD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AFO85453.1_TD.1"
                     /evalue="2.70E-82"
                     /label="AFO85453.1_TD.1"
                     /locus_tag="AFO85453.1"
                     /protein_end="2735"
                     /protein_start="2490"
                     /score="267.5"
                     /tool="antismash"
                     /translation="VLLTGATGFLGAALLDQLLRHTDATVHCLVRALDRDAGLARIRDN
                     LHRHGVELDPARAARVVAVPGDLARPRLGLSEERFHALAELLDAVYHNGAGVSAVEPYS
                     RLRAGNVLGTHEVIRLATRVRAVPVHFVSTAAVAVSSDDGDGPFLEGQRAPSASLLPSG
                     YVATKWAAEGLVRAAGERGLPVTIHRPGRISGYSRTGVGGSGDTLWQLVRAMLLLGAVP
                     RSAEHDAAMVDMVPVDHVAGAIV"
     CDS             9764..11110
                     /codon_start=1
                     /experiment="gene disruption"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 443.2; E-value:
                     1.3e-134)"
                     /gene_kind="biosynthetic-additional"
                     /note="MpnC"
                     /product="P450-like monoxygenase"
                     /protein_id="AFO85454.1"
                     /sec_met_domain="p450 (E-value: 1.7e-15, bitscore: 45.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLFADPEGSYVLLVNGDGRRSAWPASAPLPPGWRSSDESDALP
                     ERGCPAAGPPDPVPYPFRDYHRLDVDPVYAELRADRPVLRIRPPYGDDAWLVTRYDDVK
                     TVLGDPRFSRALTAHNDESRLTPRPVNTSILGTDPPDHTRLRRLLARAFTARRAELLRP
                     GVRAFTATLIDEMTKRGAPADLVEDFALPLTALVICELLGVPYADRPAFRVWLDAFSST
                     TALSVEQINSRTEALYAYIDGLVAQRRSDPAGDLISALVDAHDETAGITGQELVELVSV
                     LLIAGYETTSAQLANSVLVLFTHPHAHEALRADPGLLPGAVEELIRFVPLDAHVAFGRY
                     AREDVELSGVRIRAGEAVLPVLPSANRDPSTFPRPDELDLCRGRNPHLGFGYGIHRCIG
                     APLAKVVLEEGLGGLLARFPGLSPAVPPEEMVFKEGVQVRILQTLPVTW"
     CDS             11167..12291
                     /codon_start=1
                     /experiment="gene disruption"
                     /gene_functions="biosynthetic (rule-based-clusters) indole:
                     indole_PTase"
                     /gene_kind="biosynthetic"
                     /note="MpnD"
                     /product="aromatic prenyltransferase"
                     /protein_id="AFO85455.1"
                     /sec_met_domain="indole_PTase (E-value: 5.1e-77, bitscore:
                     248.1, seeds: 22, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGDPFVDNGTVSSQRPLRAVPGRYPPGATHLDAAVDTLVRCHAA
                     LGRAPSEAEAAVCLLRRLWGRWGNTPVERPGWRSYVAVDGSPFELSAAWNGDGPAEVRV
                     TVEATADPPTPEGNQEAGWEYLRGLSRHPGAATARVLALEDLFRPQTPHDRCWIMHGMA
                     SRPGADPLFKVYLDPDARGAAEAPSVLDEAMDRLGVRAAWQGLRGWLDEHGGSGRIGSL
                     ALDLADTDDARVKVYVQHAGLDWADIDRQAAVARGHVPGAFSAALEEITGTEVPPHKPP
                     VTCFAFHRGVGVPTAATLYIPMPAGVPESDARRRSAAFMRRSGLDSAAYLAFLAAATGD
                     GEGVRALQNFVAYRPAAPGGRPRFACYVAPGLYR"
     CDS             12357..13109
                     /NRPS_PKS="Domain: PKS_KR (14-176). E-value: 2.8e-16.
                     Score: 51.8. Matches aSDomain:
                     nrpspksdomains_AFO85456.1_PKS_KR.1"
                     /codon_start=1
                     /experiment="gene disruption"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     252.2; E-value: 8.5e-77)"
                     /gene_kind="biosynthetic-additional"
                     /note="MpnE"
                     /product="reductase/oxidase"
                     /protein_id="AFO85456.1"
                     /sec_met_domain="adh_short (E-value: 6.8e-38, bitscore:
                     119.3, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2.9e-34, bitscore:
                     108.0, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQRASGALKGRIAVVTGASRGIGRAIALRLARDGAAVVAGYHQD
                     KAGADAAVAEIEAAGGRAQAVRVDLADLADIERLFDVAVARFGAVDILVNNAGTVTAGP
                     IAAVTEAEYDRVMAVNTKGVLFALRQAGLRLRDGGRVINVSSLNTVMPEAEAAVYTGSK
                     AAVEGFTRVAAREFAERGITVNTVSPGPVQTDLLKGNPPDVLEQIAADTPLGRLGRPSD
                     IADVVAFLAGPDARWLTGQNLRATGGLA"
     CDS_motif       12399..12467
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AFO85456.1_0001"
                     /evalue="4.90E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="AFO85456.1"
                     /protein_end="37"
                     /protein_start="14"
                     /score="16.0"
                     /tool="antismash"
                     /translation="VVTGASRGIGRAIALRLARDGAA"
     aSDomain        12399..12884
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AFO85456.1_PKS_KR.1"
                     /evalue="2.80E-16"
                     /label="AFO85456.1_PKS_KR.1"
                     /locus_tag="AFO85456.1"
                     /protein_end="176"
                     /protein_start="14"
                     /score="51.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VVTGASRGIGRAIALRLARDGAAVVAGYHQDKAGADAAVAEIEAA
                     GGRAQAVRVDLADLADIERLFDVAVARFGAVDILVNNAGTVTAGPIAAVTEAEYDRVMA
                     VNTKGVLFALRQAGLRLRDGGRVINVSSLNTVMPEAEAAVYTGSKAAVEGFTRVAARE"
     CDS             13133..13654
                     /codon_start=1
                     /experiment="gene disruption"
                     /note="MpnF"
                     /product="Isopentenyl-diphosphate delta-isomerase"
                     /protein_id="AFO85457.1"
                     /transl_table=11
                     /translation="MVLVDDEGRPTGVAPKATVHTTDTPLHLAFSCYILGRQGRVLVTR
                     RALAKRTWPGVWTNSCCGHPGPGEPTPAAVRRRVREELSLELSDLAEVLPDFRYRATAA
                     DGTVENEICPVYLARTDTDPVPAVAEVAEWRWVDWSELVTLAETAPWAVSPWAALQIPL
                     LARAGVVGEP"
     CDS             13713..14231
                     /codon_start=1
                     /experiment="gene disruption"
                     /note="MpnG"
                     /product="Acetyltransferase"
                     /protein_id="AFO85458.1"
                     /transl_table=11
                     /translation="MLIRRERPADIDAIRAVTTAAFNRPGLDGEPVETGLLEELRADPG
                     WLPGLSFVAQADTGEVVGHVVCTRGQVGPVPALGLGPLSVVPGHQRAGVGSALMHAVLG
                     AADALDEPLVALLGEPAYYGRFGFRPAREEGVIAPDPAWGDYFQVRLLTAHTPRARGAF
                     GYAEPFTRL"
ORIGIN
        1 atgacgacct cagacgacat cccgcccctg cccgaggact ggcagcgcgc gttggccgtg
       61 gtggcgcacc ccgacgacct ggagttcggc gcctccgccg cgatcacccg ctgggtgggg
      121 cagggccggc aggtggcgga gctcctggtg acgcgaggcg aggcgggcat cgacacgatc
      181 gccccgcagg agtgcggacc gctgaggacg gcggagcagc tcgccgccgc ccgtgtggtg
      241 ggggtggagg aggtcgcctt cctcgacttc cccgacggtt ccctgcagta cgggctcgac
      301 ctgcgccgcg agctggcccg cgccattcgc agacaccgtc ccgaggtgct catctcgctg
      361 aacttccgcg agacctttcc gggcagcgcc gcgttcaacc acgccgatca ccgtgccctg
      421 gggcccgccc tcctggacgc cgtccgtgac gcggcgaacc gttggatgtt ccccgacctg
      481 tccgacgagg ggctgcgacc gtggtcgggc gttcgcttcg cggcgttctc cggctcccct
      541 cgggcgaccc atcacgtccc cctcagcgaa ggggaactgg ccgcggggat cgcctcgttg
      601 gccgcgcacc gcgcctacct ctccgcgctc gggggattcg accacgaggc gttcctgacc
      661 gccgaggcgc gttcggcggg cgagtcctgc gggacggggc tggccaccct gttcgaggtg
      721 atccccacct gatgttcggg gatagggaac tgaggtaagt cggtatttca agccgtccct
      781 atccctcttg tgtgctcggg ggagggacag gaatcctccg attcgggaaa accctttcta
      841 cggttcaacg gcacctcggc ggacggccgg ggtgccgttc tcgtccgtgg tacgcggagg
      901 agcaacgaat tcatcttttc tcaccgtgga tggtgaccta gaaaatatct tgtgacatgt
      961 cgggttttgt gttgctcgcg gcatacttgc gaacctggtt gggcattccc ggctcggatt
     1021 ttccctctgt ggagggtgag ggggatcgtt tatcgtgatt tcctgatggg taactcgatt
     1081 cgggtgcctg ctcccccttg ggacattttt gaatttgttt cactctgatg ttgggagtgg
     1141 gcctagtgtt ccaccgatcg acgggatcga tcttcgtgtg caattcatat ggaccgtggt
     1201 ctccgctcac ctccgcccaa aacgccgtgc tggagtcatg gcgtgctgcg cgccacgcac
     1261 cccaggtgtg cgcggtgggg gagtacgtcg acatccgcgg cccgttggac ccgaccctct
     1321 tcaccacggc gttccgtagg gcggccgagg agaccgacgc gctccgcctg gggttcgccg
     1381 agatcgacgg ggagatccgg caacgcctca ccacctcccc gacgcccgcc ttccggcgga
     1441 tcgacctcac ccacctcgcc gatccctggg aagaggtcca cgggctcctc gcgcgcgagc
     1501 gccttctccc acggctcccc gccgcccccc acgaggtgta cgaccacgcc ctggtccgac
     1561 tcgcccccga ccgtctcctg tgggtccacc acgcccaccc ccttgccctg gaccggacgg
     1621 ggctcgccgc gctgcgctcc cgcgtcgccg cgtactacac cgacctcgcc ggcggaccgc
     1681 cggccgacgc cacccgaccg tcccggtccc ccgacaccga ccggcccatc ggggcgcgtc
     1741 ctccccgcgg acacagcgga gccctgccgg cgggcctgcg ggaccgcctg gggaccttcg
     1801 ccgccgaccc gggggccgcc gtcctcgacc cgttcctggc cgccgcggcg ctccacggcc
     1861 gagacggcca cgacgaggcc gtggtcggcc tggtcaccgg ggaacccgac caacgagggc
     1921 gggccgcgcc ctcccacgcg cgacgtcacg ccctccgact cgccgccgac ccccacgtga
     1981 gcgcggccga gctgctgcgg gccgcctccc tgctgcgccg cgacgccgcg acgcgcccgg
     2041 tcgacccgcc ccgcgacggg gaggagacgg cgcgcaagcc tccccgcgtg gtggtcgaga
     2101 ccgacgcctc ggtcgtgttc ctcggccccc accgaaccac cgtccacacc ctcgaccccg
     2161 gcaacggcga ccacaccgcg gtcatcgtgc gcgaggacac cgtcgaactc ttcatcggcg
     2221 cgggggccga ccaggccgcg gacccgacgc tccagcaccg ccggctcctc gagctggtcg
     2281 cccaggtcct cgccaccccc gagacgggca ccgcgcaggc ccgcgcgacg gctcccgcgg
     2341 agcagccggg aaggccgccc gaggcgaccg gcgccccctc ctcggagccc tgggcctgcc
     2401 tacccgacct gttcctacga caggcgcgtc gagaccccga ccgcgtcgcc atcgtggacg
     2461 gcgacgagca gatgagctac gccgagctcg acgcccggtc caaccgcctc gcgcaccgcc
     2521 tggtccaccg cggctgttcc cccggggcca gcgtcgggct cgcgcttccc cgatcggccg
     2581 acctcctcgt ggccaccctc gccgtactca aggccggggc cgcctacgtt ccactggacc
     2641 tgcgctcccc ggggccgcga ctgcgccgga tcctccagga gaccggcacg cggatcgtca
     2701 tcaccgagcc cgggacgcac ggacatgacg ccttcgccgg gatggccctc ctccccgtcg
     2761 gccccgaccc cgccggcgcg gacccgggca cgccccgaag cgctctccac cccgacagcc
     2821 tcgcctacgt catgtacacc tccgggtcca ccggagcccc caaggggatc gccatctcgc
     2881 atcgcgcggt ggcggcgctc gcggccgacc ggcgatggga cggaggcgcg cacgaacggg
     2941 tgctcctgca ctccccgcac gccttcgacg cctcgaccta cgagatctgg gtccccctgc
     3001 tgaggggcgg caccgtggtc gtcgcgcccc cgggcgagac cgatcccgtc cgactgcgcc
     3061 ggctcctgcg cgcggagcgc atcggcgcgc tgttcgtcac caccgccctg ttctccctcg
     3121 tcgccgagga gctgcccgac tgcttcgcca cggtccggga ggtgtggacc gggggcgagc
     3181 ggatgccgcc gaaggcggtc gagcgggtgc tcgaccactg tccacgactc cacctggtcc
     3241 acgtctacgg tcccaccgag tccacgacgt tcgcgacctg tcacccggtc ccccgggccg
     3301 aggtgggcga tccggtgccg atcggcggcc ccatggacaa cacccgctgc cacgtcctcg
     3361 acgaccaatt gcgcccggtc ccgcccggcg tcccgggaga gctctacctc gccgggatcg
     3421 gtctggcgcg ggggtacacg ggagccgcgg gcctcaccgc cgaacgcttc gtcgccgaac
     3481 cgtccggtcc ccctggatcg cggatgtacc gcaccggcga catcgtgcgc tggagggcgg
     3541 acggcgccct ggagttcctg caccgggccg acggacaggt gaagatccgg gggtttcgca
     3601 tcgagccggg cgaggtcgag aaggcgctgt gcgcccatcc gggggtcagc gcggccgcgg
     3661 tcgtggcgcg cgaggagcgg cccggcgagc gcaggctcgt cggctacatc gtgcccgccg
     3721 accgcgacga gggggccgcc gacggcgagg gcgaccagat cggcgagtgg agggggatct
     3781 acgaccggtt gtacacggcc ccggccgaga cccccctcgg gaccgacttc accggctgga
     3841 acagcagtta cgacggcacc gccatccccg aggaacagat gcgcgaatgg cgcgacgcca
     3901 cggtggagcg catccgggcc ctgcgcccgc ggcgcgtgct ggagatcggg gtggggaccg
     3961 gcctgctgat ggcgggactg gccccccact gcgagtcgta ctgggggacc gacttctccg
     4021 cccccgccat cgaggggctg cgccgcggga tcgcccgcga tcccgccctc gcccggcggg
     4081 tcgagttgag ggtgtgcgcg gccgacgagc tcggcggact gccggccggc ttcttcgaca
     4141 ccgtcgtcat caactccgtc acccagtact ttcccaacaa cgggtacctc acccgggtgc
     4201 tggccggggc ggccgggctg ctcgccccgg gcggggcgat cttcgtgggg gacgtgcggg
     4261 acctgcgact cgcccgcccc ttccacaccg aggtcgcgct gcgccgcgcg gcccccggcg
     4321 acgacgcggc ggccgtgcgc gccaccatcg agcgcgacag cgccgccgag caggagctca
     4381 tggtctcccc ggactacttc atggcgctgt gctcccacgt cccccggctg gccggggccg
     4441 acatccaggt caagggcggc cgccaccgca acgagatgac gcagtaccgc tacgacgtcg
     4501 tgttacgcgc cgaccggccg accacgccgg aggaggacgt cgacgtccgc accctcgtct
     4561 ggggcaggga cctggccgga ctcgatgagc tggccgggct gctggccgcc cgtacccacc
     4621 ggcacgtccg tctgctcgac gtgcccaacg cgcgcgtcgc accggccgcg ctggcctggc
     4681 gggcgctgag cgaggagcgg gacgccgacc tcgcccggcg gcgtctggac gaggccgggc
     4741 gaggactccc cgaacccgcg gagctcgagg aggtcgggcg ccttctgggg ctgcggacgg
     4801 cgctgacctg ggccgcggac gcctccgacg gagcgctcga cgcgcacttc ctggccctgg
     4861 gagccgagga gcccgtgccc tgcgggctgt accgttcgcg gggcacggcc gacgcgcccc
     4921 tgcgcgccca caccaacagc ccccgtacct cccgcgaggc cggcgccctg gtcgcctcgg
     4981 tgcgcgacct cctcggcgag cgccttcccg actacatggt gccccaccac atcgtcgtcg
     5041 tggaccggct tcccctgacg cgcaacggca agctcgaccg cgcggcgcta ccggcgccga
     5101 aaccggggac gacccttgac gggcgcgccc cccggacccc gacggaggag cggctgtgcg
     5161 ccctctacac cgaggtgctc ggagtctccc cggtcagcat cgacgacgac ttcttcgagc
     5221 tggggggaca ctccctgtcg gcgacacggc tggtcgcgag gatccgggcc gggctggagg
     5281 tcgaggtccc gttgcgcgcc gtcttcgaga ccccgcgggt cgtcgacctc gtcgaacgcc
     5341 tcggccggac agcggcccgg cccgccctga cccaccgggc gcgccccgag cgcgtccccc
     5401 tgtccccggg acaggcgcgc ctgtggttcc tgcatcggtt ctcgggcgcg gaccccctct
     5461 acaacgtgcc gtttcggatc aggctctcgg gaccgctcga ccacgccgcg ctgcacgcgg
     5521 cactgtgcga cctggtggac cggcacgaga gcctgcgcac ggtcttcccc gagcacgagg
     5581 gcgtccccta ccaactcgtg accgaggcac aggtacggct gccggtcgtc gccagcacgc
     5641 cgcaacgggt cgaggcggac gcgcgtgcgg cggctcggtg cgggttcgac atcgccacgg
     5701 agatcccgtt ccgggcggtc ctcttccgcc tcgccgacga cgcgcacgtc ctcctgatcg
     5761 tcgtgcacca catcgcctgc gacgggtggt cgctgggccc cctgtggcgc gacctggcca
     5821 ccgcctaccg cgagcgcctg gcggggcggc gccccttcga ccgcccgctg ccggtccaat
     5881 acgccgacta caccctgtgg cagcgggacc tcctcgacga agggaacccc gaggggacgg
     5941 ccgagacgca actgcgccac tggcgggagg gcctggccgg actgcccgac cggatcgtgt
     6001 tgcccaccga ccggccccgc cctccgctcg cccggcacag cggcgcgacc gtccgcttct
     6061 cggtgcccgc cgccctgcac gaccggctcg tctcggtggc gcaggagcac ggggccaccg
     6121 tgttcatggc gctgcacgcc ggcctggccg ccctgctgtg ccggctgggc ggtggaagcg
     6181 acatccccgt cggtagtccc atcgccgggc gcaccgaccc cgccctggac gacctcgtgg
     6241 ggttcttcgt caacaccctg gtgatccgga ccgacacctc gggcgacccg tcgttcgccg
     6301 aactcctggg ccgggtccgc gaggtcgtcc tcgccgcgca cgaacaccag gacctgccct
     6361 tcgagcgact ggtcgaggag ctcaggccgg cgcgctccct cgcccaccac ccgctcttcc
     6421 aggtcatgct ggccctccag aacgcgcccg gatcggattt ccggctggcc ggcctcaccg
     6481 ccgaggccga cctgatggac atcgggacct cccggttcga tctggttctc agcctcaccg
     6541 aacgctttgc ggacgacggg accccacagg ggatggacgg gatcgcggag ttcgacaccg
     6601 acctgttcga tcccgggagc gtcgatgcgc tggtgcggcg gctgctccgg ctgctcgacc
     6661 gcgtcaccgc gcggcccggt ctgcggatcg gagaggtcga cctcctcgag gggacggaac
     6721 accgtcggct gctgcgggag tggaacgaca gcggaggacg gaccgccccc gcgaccttcc
     6781 ccgagctgtt ccagcgccag gtcaccgcgg ctcccgaggc caccgcgctg gtccacgggg
     6841 aggacacgtg gtcctaccgg gccctcaacg cccgggccaa ccggttcgcc cgcgccctca
     6901 tccgatacgg cgccggcccc gagacccacg tcgccgtcgt cctgccccgc tcggccgagc
     6961 tggtcacggt cctggtcgcc atcatgaaag ccggcgcggc ctatctcccc ttcgaggccg
     7021 cgcagcccgc ccggcgtctt gaggcgatgc tcgccgaggc ccggccggcg ctggtcatcg
     7081 gcacggccga caccctcgcg gagctgcccg gagactcacc cgttctcccc ctcgaccgcc
     7141 ttgaggccga ggccgccctc ctcgccgaca acgacgtgac cgacggggaa cggggcacgc
     7201 ccctgctgcc cgaccacccc gcctacctga tctacacctc gggctccacc ggacggccca
     7261 agggggtcgt ggtcacccac agcgggttct cggggatggc cgacatccaa cgcgaacgtt
     7321 tcggggtgac ccgtgacagc cgcgtcctcc agttcgcgcc gatctccttc gacggcgccg
     7381 tgtgggagat cttcgggacg ctggccgtgg gggcgaccct cgtcaccgcg ccggccgacc
     7441 aggtcatgcc gggtcccgac ctggtccggc tgctgcgcgc gcgggggatc acccacctca
     7501 ccctgccccc cacggccctg gccgtcatgg agcccgccga cctcgacggc gtctcctccc
     7561 tgatcgtctc gggcgaggcg tcctcgggag cgctcgtcgc cgcctggtcg ccggggcggc
     7621 ggatgctcaa cggctacggc cccaccgaga ccacggtgtg cgccacgctg agcgaccccc
     7681 tggccggcgc ggcgccgccc cccatcggcc gtcccgcgat cggcgcccgc tgttacgtgc
     7741 tcgacgaccg gctgcggccg gttccccccg gttcccccgg agagctctac gtcgccgggg
     7801 ccgggctggc ccggggttac ctcggcgatc cggggcgaag cgccgccagg ttcgtcgccg
     7861 acccgttcgg cgttccgggg gagcggatgt accgcacggg cgacctcgcc cgctggaccc
     7921 gcgggggagt cctggagttc ctgggacgcc gcgacgagca ggtgaagatc cgcggtttcc
     7981 ggatcgagcc cggcgaggtc gcggcggcgc tcgaacggca tccgtcggtc gggcgcgcgg
     8041 ccgtggtcgc gcgcgaggac cggccggggg accggcggct ggtggcctac gtcgtctcca
     8101 cgggggagcc cgtcgacgcc gccgaggtgc gtcggcacgc cgccgacctg cttcccgccc
     8161 acctcgtgcc ctcggtggtc gtcccgatcg cggcactgcc gaccctgccc agcggaaagg
     8221 tcgaccgggc cgcgctcccc gcccccgtcg cggtcacggt gtcgcgagga cgccggccga
     8281 gcagcccgct cgagcacacc ctgtgcggac tcttcgagga actcctcggc gtgcccggga
     8341 tcacggtgga cgacggcttc ttcgacctgg gcgggcactc cctgctcgtc ccgcgtctcc
     8401 tgcggggcgt ccgtgaccgc accggcgccg acctgagcgt ggcgcagttg ttcgagacgc
     8461 cgaccgtcgc ggggctcgcc cacgagatcg gcggtggccg gggcgggcgg gcgaggccgt
     8521 ccgagcccga tcccgccgcc gacaccgttc tcgacccgct tgtcaaggtc cccgcggacg
     8581 ggatcgacct cgcgcgcaac gccgctcccc gccacgtcct gctcaccggg gccaccgggt
     8641 tcctcggcgc agcgctgctc gaccagctgc tccgccacac cgacgccacc gtgcactgcc
     8701 tggtccgcgc cctcgaccgg gacgccggcc tggcccggat ccgcgacaac ctccaccggc
     8761 acggagtgga actggacccc gcgcgtgccg cgcgcgtggt ggccgtcccc ggggacctcg
     8821 cgcggccccg gctgggcctg tccgaggaac ggttccacgc cctggccgag ctgctggacg
     8881 ccgtgtacca caacggggcc ggggtgagcg cggtcgaacc ctactcccgg ttgcgggccg
     8941 gcaacgtgct cggcacccac gaggtcatcc ggctggcgac gcgggttcgg gccgtcccgg
     9001 tgcacttcgt gtcgaccgcg gcggtcgcgg tgtccagcga cgacggggac ggccccttcc
     9061 tcgaagggca gcgggccccc tccgcctccc tcctgcccag cggttacgtg gccaccaagt
     9121 gggcggccga ggggctggtg cgcgcggcgg gagaacgcgg gctgcccgtc accatccacc
     9181 gtcccggccg gatcagcggg tactccagga ccggcgtggg gggttcgggg gacacgctgt
     9241 ggcaactcgt ccgggccatg ctgctgctcg gcgcggttcc gcgcagcgcc gaacacgacg
     9301 cggccatggt cgacatggtt cccgtcgacc acgtcgccgg ggccatcgtc catctcgcgc
     9361 ggcggcccga ggccatggga cgtgtgcacc acctgacctg tcccaggccg atccccttcc
     9421 cccggctgct cgccctgttc cgcgacaggg gctacgtcct ggccccgctc gccgacgagg
     9481 agtgggccga gctgctgctg cgccgggccg aggaccccga cacgggcacc ggcctggcgg
     9541 ccgccgcgct cctcgtcgag gcccttcccg gcatcgtcag gctcggcgcg ctgcggttcg
     9601 accggcgcaa caccctgcgg ggactggcgg aggccggatg ggacctctcc ggagccgagc
     9661 ccgtgctgct cgaacgctac atcgaccacc tcgtccgcag cggtgtcttc cccgcgccgg
     9721 cgcgaacccc ccaacaaccc ccgacaagcg aaagggccga ggtgtgaaca acctcttcgc
     9781 ggaccccgaa ggaagctacg tgctcctcgt caacggcgac gggcgccgct cggcctggcc
     9841 ggcctccgcg ccgctcccgc ccggctggcg gtcgtccgac gagagcgacg ccctccccga
     9901 gcggggctgt ccggcagcag gaccccccga cccggttccc taccccttcc gggactacca
     9961 ccggctcgac gtggaccccg tctacgccga gctccgcgcc gaccgcccgg tcctgcggat
    10021 ccggcctccc tacggggacg acgcctggct cgtcacccgc tacgacgacg tcaagaccgt
    10081 cctcggcgat ccccggttca gccgggcgct gacggcccac aacgacgagt cgcggctcac
    10141 cccccggccc gtcaacacca gtatcctggg aaccgacccg cccgaccaca cgcggctgcg
    10201 ccggctgctc gcgcgggcct tcaccgcgcg gcgggccgaa ctgctccgcc cgggcgtgcg
    10261 cgcgttcacc gccaccctca tcgacgagat gacgaagcgg ggcgcgccgg cggacctggt
    10321 cgaggacttc gcgctccccc tgaccgccct ggtcatctgc gaactcctcg gcgtgcctta
    10381 tgcggaccgg cccgccttcc gcgtctggtt ggacgccttc tccagcacca cggcgctgtc
    10441 cgtcgagcag atcaactccc ggaccgaggc gctgtacgcc tacatcgacg gtctcgtcgc
    10501 ccagcgccgg agcgacccgg ccggcgacct gatcagcgcg ctggtcgacg cgcacgacga
    10561 gaccgcgggc atcaccggcc aagaactcgt cgagctcgtc agcgtcctgt tgatcgccgg
    10621 ctatgagacg acctcggcgc agctggccaa ctccgtcctc gtcctgttca cccaccccca
    10681 cgcccacgag gcgctgcgcg ccgatcccgg cctcctgccc ggcgccgtgg aggagctgat
    10741 ccgcttcgtc cccctcgacg cccacgtggc cttcggccgc tacgcccgcg aggacgtcga
    10801 gctgagcggg gtccgcatcc gcgcgggcga ggccgtgctg cccgtgctgc cctcggccaa
    10861 ccgcgacccc tcgacgttcc cccggcctga cgaactcgac ctgtgccgtg gccgcaaccc
    10921 ccatctgggg ttcggctacg ggatccaccg ctgtatcggc gcgccgttgg cgaaggtcgt
    10981 gttggaggag gggctgggcg ggctcctggc ccgtttcccc gggctgtccc cggcggtgcc
    11041 gcccgaggag atggtcttca aggagggggt ccaggtccgg atcctccaga ccctccccgt
    11101 gacatggtga gcccgcgcgg atccgaacgg ggccctcggg agacggcgga cacgtccacg
    11161 ggcgtgatgg ccggcgaccc cttcgtcgac aacgggacgg tctcctccca gcgcccgttg
    11221 cgggccgtcc ccggccggta cccgccggga gccacccacc tcgacgccgc cgtcgacacc
    11281 ctggtccgct gtcacgcggc cctgggccgg gcgccgtccg aggccgaggc ggcggtctgc
    11341 ctgcttcggc ggctgtgggg ccgttggggg aacaccccgg tcgagcggcc cggctggcgc
    11401 agctacgtgg cggtcgacgg ttcccccttc gagctgtccg ccgcctggaa cggtgacggg
    11461 ccggcggagg tgcgcgtcac cgtcgaggcg acggccgacc cgccgacgcc cgaggggaac
    11521 caggaggccg gctgggagta cctgcgggga ctctcccggc acccgggggc cgccaccgcg
    11581 cgcgtcctcg cgctggagga cctcttccgg ccccagaccc cgcacgaccg gtgctggatc
    11641 atgcacggca tggccagccg gccgggcgcc gatcccctgt tcaaggtcta cctcgatccc
    11701 gacgcccggg gggcggccga ggcgccgtcc gtcctcgacg aggcgatgga ccggctcggt
    11761 gtccgggccg cctggcaggg actacggggt tggctggacg aacacggtgg ttcgggccgt
    11821 atcggttcgc tcgccctcga tctcgccgac accgacgacg cgcgtgtgaa ggtctacgtt
    11881 cagcacgccg gcctggactg ggcggacatc gaccgacagg cggcggtggc ccgggggcac
    11941 gtgcccggag ccttctcggc cgcgctggag gagatcaccg gcacggaggt tcctccccac
    12001 aaacctcccg tcacctgctt cgccttccac cgtggcgtgg gggtgccgac ggccgcgact
    12061 ctctacatcc cgatgccggc gggggttccc gagtccgatg cccgccgaag aagcgccgcg
    12121 ttcatgcggc ggtcgggcct ggattccgcc gcctacctcg cctttctcgc cgctgccacg
    12181 ggggacggcg aaggtgtgcg ggcgcttcag aacttcgtgg cctaccggcc cgccgcccct
    12241 gggggacggc cccggttcgc ctgctacgtc gccccggggc tttaccgctg acggtcccgg
    12301 gccggccctg tccgacagcc ctggacggaa agggacacat gtcttatcac gaacaagtgc
    12361 cgcaaagggc atctggtgcg ctgaagggaa gaatcgcggt cgtcaccgga gcatcgcggg
    12421 ggatcgggcg ggccatcgcc ctgcggctgg cgcgtgacgg cgccgcggtg gtggccggat
    12481 accaccagga caaggcgggg gcggacgcgg ccgtcgccga gatcgaggcc gcgggtgggc
    12541 gcgcccaggc ggtccgggtc gacctggccg acctcgccga catcgagcgg ctgttcgacg
    12601 tcgcggtcgc gcggttcggc gccgtcgaca tcctggtcaa caacgctgga acggtcaccg
    12661 ccggccctat cgccgccgtc accgaggccg aatacgaccg ggtgatggcg gtcaacacca
    12721 agggggtcct cttcgccctc cgccaggccg gactgcggct gcgcgacggg ggacgtgtca
    12781 tcaacgtctc ctcgctcaac accgtcatgc ccgaggccga agccgccgtc tacaccggta
    12841 gcaaggcggc ggtggaggga ttcacccgcg tcgcggccag ggagttcgcc gagcggggca
    12901 tcaccgtcaa caccgtctct cccggtccgg ttcagaccga tcttttgaag ggaaacccac
    12961 ccgacgtgct cgaacagatc gccgcggaca ccccgctcgg ccggctcggc cgccccagcg
    13021 acatcgccga tgtcgtggcc ttcctcgccg gtccggacgc ccgttggctc accggccaga
    13081 acctgcgcgc cacgggagga ctcgcgtgag gggcaccgaa gaggtcgaat cggtggtgct
    13141 ggtcgacgac gagggacggc ccaccggcgt cgcgcccaag gccaccgtgc acaccaccga
    13201 cacaccactg cacctggcct tctcctgcta catcctggga cggcagggaa gggtgctggt
    13261 gacccgcaga gcgctcgcca agcggacgtg gccgggggtg tggaccaatt cctgctgtgg
    13321 ccatccaggg ccgggtgaac cgacgcccgc ggccgtacgc cggcgcgtcc gcgaggagct
    13381 ctccctggag ctgagcgacc tcgccgaggt cctgcccgac ttccgctacc gggcgaccgc
    13441 ggccgatggc accgtcgaga acgagatctg tcccgtctac ctggcgcgca ccgacaccga
    13501 cccggttccc gccgtggccg aggtggccga gtggcggtgg gtggactgga gcgagctggt
    13561 caccctggcc gaaaccgccc cgtgggcggt gagcccgtgg gccgcgttgc agatcccgtt
    13621 gctggcccgg gcgggcgtgg tcggggagcc gtgaccgggc ttaccggggc ggcccggaac
    13681 cggccacgtc cgtggtggga cacaatgatc ggatgctgat ccgacgcgaa cgtcccgccg
    13741 acatcgacgc catccgggcc gtcaccaccg cggccttcaa ccgtcccggt ctcgacggcg
    13801 aacccgtcga gaccggcctg ctggaggagc tgcgggccga ccccggttgg ttgcccggac
    13861 tgtcgttcgt cgcccaggcg gacacgggcg aggtggtcgg ccacgtcgtg tgcactcgag
    13921 gccaggtcgg tccggtgccc gcgctggggc tggggccgtt gagcgtcgtc cccggccacc
    13981 agcgcgcggg ggtggggtcg gcgctcatgc acgcggtgct cggcgccgcc gacgcgctcg
    14041 acgagccgct cgtcgcgctg ctgggggagc ccgcctacta cggcaggttc gggttccggc
    14101 cggcccgcga ggagggggtc atcgcgcccg atccggcgtg gggcgactac ttccaggtgc
    14161 gcctgctcac cgcccacacg ccgcgggcgc ggggcgcgtt cggctacgcc gagccgttca
    14221 cgcggttgtg a
//
