BGC0000397 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000397 (Nostoc sp. ATCC 53789)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	34,328	nostocyclopeptide A2	NRPS:Type I	100%

BGC0000397 - Region 1 - NRPS

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Location: 1 - 34,328 nt. (total: 34,328 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 34,329
Label		BGC0000397

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000397	1.00	NRP	nostocyclopeptide A2	Nostoc sp. ATCC 53789
BGC0001133	0.44	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.40	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.35	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002437	0.34	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001844	0.34	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002518	0.33	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0002706	0.33	NRP	saframycin Mx1	Myxococcus xanthus
BGC0001131	0.33	NRP	ambactin	Xenorhabdus miraniensis
BGC0000331	0.32	NRP	cyanopeptolin	Planktothrix agardhii NIVA-CYA 116

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000397	1.00	NRP	nostocyclopeptide A2	Nostoc sp. ATCC 53789
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.72	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.68	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002437	0.67	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001844	0.67	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0002706	0.66	NRP	saframycin Mx1	Myxococcus xanthus
BGC0001131	0.66	NRP	ambactin	Xenorhabdus miraniensis
BGC0000331	0.66	NRP	cyanopeptolin	Planktothrix agardhii NIVA-CYA 116

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 34328:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Tyr - Gly - D-Gln) + (Ile - Ser - X - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

ncpA

Search NORINE for peptide: strict or relaxed

AMP-binding (535..936): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DASTVAAVCK
Nearest Stachelhaus code(s):: Tyr DASTVAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1596..1997): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2671..3080): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLIDK
Nearest Stachelhaus code(s):: Gln DAWQFGLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

ncpB

Search NORINE for peptide: strict or relaxed

AMP-binding (486..913): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: ile (Ile)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFFLGVTFK
Nearest Stachelhaus code(s):: Ile DAFFLGVTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1587..2010): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHISLIDK
Nearest Stachelhaus code(s):: Ser DVWHISLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2674..3142): X

nrpys: 4Me-Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVAK
Nearest Stachelhaus code(s):: 4Me-Pro DVQFIAHVAK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3804..4217): X

nrpys: Leu, Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTIGGVCK
Nearest Stachelhaus code(s):: Leu or Phe DAWTIGGVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)