BGC0000398: orfamide B biosynthetic gene cluster from Pseudomonas sp. CMR12a
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Location: 1 - 45,376 nt. (total: 45,376 nt).
This entry is originally from NCBI GenBank JQ309921.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000398
Short description orfamide B biosynthetic gene cluster from Pseudomonas sp. CMR12a
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Remarks "orfamide A and B possess a 3-OH-tetradecanoic acid lipid moiety, while orfamide C possesses 3-OH dodecanoic acid"
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
Loci NCBI GenBank: JQ309921.1
Compounds
  • orfamide B
Species Pseudomonas sp. CMR12a [taxonomy]
References
Chemical products information
orfamide B
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C63H112N10O17
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AFH75326.1
 2082 - 2816 (+) putative acetyltransferase
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  • AFH75327.1
 2951 - 3751 (-) LuxR family transcriptional regulator
  • Regulation
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Sequence-based prediction
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  • AFH75328.1
  • ofaA
 4357 - 10734 (+) nonribosomal peptide synthetase
  • Scaffold biosynthesis
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  • AFH75329.1
  • ofaB
 11026 - 24123 (+) nonribosomal peptide synthetase
  • Scaffold biosynthesis
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  • AFH75330.1
  • ofaC
 24120 - 38810 (+) nonribosomal peptide synthetase
  • Scaffold biosynthesis
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  • AFH75331.1
  • macA
 39000 - 40121 (+) macrolide-specific efflux protein
  • Transport
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  • AFH75332.1
  • macB
 40124 - 42082 (+) macrolide export ATP-binding/permease protein MacB
  • Transport
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  • AFH75333.1
 42125 - 42895 (-) LuxR family transcriptional regulator
  • Regulation
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  • AFH75334.1
 43363 - 43779 (-) glyoxalase family protein
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  • AFH75335.1
 43969 - 45375 (-) heme biosynthesis protein
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NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Thioesterase genes
  • ofaC (Unknown)
NRP-synthases
Gene Modules
ofaA
Module A1
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Starter
Module A2
Specificity: glutamic acid
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
ofaB
Module B1
Specificity: threonine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module B2
Specificity: valine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module B3
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module B4
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
ofaC
Module C3
Specificity: serine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: DCL
Module C2
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module C1
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Module C4
Specificity: valine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Dual
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: 4VPAMSFU7CTQRXZA3KNXYTFK, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Corrected NRP module activity
  • Updated bioactivity data
  • Updated NRP substrate specificities
3.1
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Update chemical activity to schema version 2.11
Detailed domain annotation
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