BGC0000398: orfamide B biosynthetic gene cluster from Pseudomonas sp. CMR12a
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Location: 1 - 45,376 nt. (total: 45,376 nt).
This entry is originally from NCBI GenBank JQ309921.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000398
Short description orfamide B biosynthetic gene cluster from Pseudomonas sp. CMR12a
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "orfamide A and B possess a 3-OH-tetradecanoic acid lipid moiety, while orfamide C possesses 3-OH dodecanoic acid"
Biosynthetic class(es)
  • NRPS (Type I)
Loci
JQ309921.1
via Knock-out studies
Compounds
  • orfamide B
Species Pseudomonas sp. CMR12a [taxonomy]
References
Chemical products information
orfamide B Evidence:
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C63H112N10O17
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AFH75326.1
 2082 - 2816 (+) putative acetyltransferase
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    • AFH75327.1
    		 2951 - 3751 (-) LuxR family transcriptional regulator
    • Regulation
    • Knock-out
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    • AFH75328.1
    • ofaA
    		 4357 - 10734 (+) nonribosomal peptide synthetase
    • Scaffold biosynthesis
    • Knock-out
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    • AFH75329.1
    • ofaB
    		 11026 - 24123 (+) nonribosomal peptide synthetase
    • Scaffold biosynthesis
    • Knock-out
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    • AFH75330.1
    • ofaC
    		 24120 - 38810 (+) nonribosomal peptide synthetase
    • Scaffold biosynthesis
    • Knock-out
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    • AFH75331.1
    • macA
    		 39000 - 40121 (+) macrolide-specific efflux protein
    • Transport
    • Knock-out
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    • AFH75332.1
    • macB
    		 40124 - 42082 (+) macrolide export ATP-binding/permease protein MacB
    • Transport
    • Knock-out
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    • AFH75333.1
    		 42125 - 42895 (-) LuxR family transcriptional regulator
    • Regulation
    • Knock-out
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    • AFH75334.1
    		 43363 - 43779 (-) glyoxalase family protein
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      • AFH75335.1
      		 43969 - 45375 (-) heme biosynthesis protein
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        Biosynthesis information

        Biosynthetic modules

        Name
        A1
        Type
        nrps-type1
        Genes
        ofaA
        Substrates
        leucine (evidence: Structure-based inference)
        Integrated Monomers
        Domains
        condensation (Starter), adenylation
        Name
        A2
        Type
        nrps-type1
        Genes
        ofaA
        Substrates
        glutamic acid (evidence: Structure-based inference)
        Integrated Monomers
        Domains
        condensation (LCL), adenylation
        Name
        B1
        Type
        nrps-type1
        Genes
        ofaB
        Substrates
        threonine (evidence: Structure-based inference)
        Integrated Monomers
        Domains
        condensation (Dual), adenylation
        Name
        B2
        Type
        nrps-type1
        Genes
        ofaB
        Substrates
        valine (evidence: Structure-based inference)
        Integrated Monomers
        Domains
        condensation (Dual), adenylation
        Name
        B3
        Type
        nrps-type1
        Genes
        ofaB
        Substrates
        leucine (evidence: Structure-based inference)
        Integrated Monomers
        Domains
        condensation (Dual), adenylation
        Name
        B4
        Type
        nrps-type1
        Genes
        ofaB
        Substrates
        serine (evidence: Structure-based inference)
        Integrated Monomers
        Domains
        condensation (Dual), adenylation
        Name
        C3
        Type
        nrps-type1
        Genes
        ofaC
        Substrates
        serine (evidence: Structure-based inference)
        Integrated Monomers
        Domains
        condensation (DCL), adenylation
        Name
        C2
        Type
        nrps-type1
        Genes
        ofaC
        Substrates
        leucine (evidence: Structure-based inference)
        Integrated Monomers
        Domains
        condensation (LCL), adenylation
        Name
        C1
        Type
        nrps-type1
        Genes
        ofaC
        Substrates
        leucine (evidence: Structure-based inference)
        Integrated Monomers
        Domains
        condensation (Dual), adenylation
        Name
        C4
        Type
        nrps-type1
        Genes
        ofaC
        Substrates
        valine (evidence: Structure-based inference)
        Integrated Monomers
        Domains
        condensation (Dual), adenylation
        Annotation changelog

        Entry version: 4

        Date
        Changes
        Submitters
        Reviewers
        2022-10-07
        Update chemical activity to schema version 2.11
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

        Entry version: 3

        Date
        Changes
        Submitters
        Reviewers
        2022-09-15
        Corrected NRP module activity
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
        2022-09-15
        Updated bioactivity data
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
        2022-09-15
        Updated NRP substrate specificities
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

        Entry version: 2

        Date
        Changes
        Submitters
        Reviewers
        2019-10-16
        Migrated from v1.4
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
        2019-10-16
        Updated compound(s) information (NPAtlas curation)
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

        Entry version: 1

        Date
        Changes
        Submitters
        Reviewers
        2015-06-12
        Submitted
        • (ID: 4VPAMSFU7CTQRXZA3KNXYTFK)
        • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
        Detailed domain annotation
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