Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000398.1:
Cluster 1NRP145376

BGC0000398, orfamide biosynthetic gene cluster from Pseudomonas sp. CMR12a. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Orfamide B
SMILES string: Copy to clipboard
Molecular formula: C63H112N10O17
Exact molecular mass: 1280.8207 Da (Molecular ion)
Molecular activity: Unknown

Class-specific details

Biosynthetic class(es):
NRP
Cyclic depsipeptide (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Unknown

Nonribosomal peptide synthetases:
ofaA (AFH75328.1)
Module A1
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Starter
Module A2
A specificity: Glutamate
Evidence for specificity: Structure-based inference
C domain subtype: LCL
ofaB (AFH75329.1)
Module B1
A specificity: Threonine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module B2
A specificity: Valine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module B3
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module B4
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
ofaC (AFH75330.1)
Module C3
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: DCL
Module C2
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module C1
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module C4
A specificity: Valine
Evidence for specificity: Structure-based inference
C domain subtype: Dual

Gene cluster description

orfamide (BGC0000398). Gene Cluster 1. Biosynthetic class = NRP. GenBank JQ309921. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Sequence-based prediction
MIxS-compliance:Unknown
Comments:orfamide A and B possess a 3-OH-tetradecanoic acid lipid moiety, while orfamide C possesses 3-OH dodecanoic acid
Contact for this cluster:Monica Hofte (Ghent University)

Literature references

1. D'aes J et al. (2014) To settle or to move? The interplay between two classes of cyclic lipopeptides in the biocontrol strain Pseudomonas CMR12a. Environ Microbiol 16(7):2282-300. doi: 10.1111/1462-2920.12462. Epub