Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000399.1:
Cluster 1NRP147020

BGC0000399, orfamide biosynthetic gene cluster from Pseudomonas protegens. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Orfamide A
SMILES string: Copy to clipboard
Molecular formula: C64H115N10O17
Exact molecular mass: 1295.8442 Da (M+H+)
Molecular activity: Antifungal, Surfactant, Other

Class-specific details

Biosynthetic class(es):
NRP
Cyclic depsipeptide (cyclic)
Thioesterase type:
Type I & II
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
ofaA (AAY91419)
Module 1
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Starter
Module 2
A specificity: Glutamate
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Dual
ofaB (AAY91420)
Module 3
A specificity: Threonine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Dual
Module 4
A specificity: Isoleucine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Dual
Module 5
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 6
A specificity: Serine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: Dual
ofaC (AAY91421)
Module 7
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 8
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 9
A specificity: Serine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: LCL
Module 10
A specificity: Valine
Evidence for specificity: Structure-based inference
C domain subtype: Dual

Gene cluster description

orfamide (BGC0000399). Gene Cluster 1. Biosynthetic class = NRP. GenBank CP000076, positions 2364934-2411953. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Proven expression in natural host, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Harald Gross (University of Tuebingen)

Literature references

1. Gross H et al. (2007) The genomisotopic approach: a systematic method to isolate products of orphan biosynthetic gene clusters. Chem Biol 14(1):53-63. doi: 10.1016/j.chembiol.2006.11.007.