BGC0000422 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000422 (Streptomyces lavendulae)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	melanin,NRPS	1	62,804	High	saframycin A/saframycin B	NRPS:Type I

BGC0000422 - Region 1 - NRPS,melanin

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Location: 1 - 62,804 nt. (total: 62,804 nt) Show pHMM detection rules used

Region on contig edge.

melanin: melC
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

Tailoring

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 62,805
Label		BGC0000422

Tailoring functions

Oxidoreductases: dehydrogenase oxidase oxygenase

sfmCy1: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

sfmCy2: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

sfmO1: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

sfmO2: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

sfmO3: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

sfmO4: cytochrome P450

smcogs: SMCOG1034: cytochrome P450

sfmO6: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Transferases: kinase methyltransferase

ABQ41261.1: methyltransferase

smcogs: SMCOG1089: methyltransferase

sfmM1: methyltransferase

smcogs: SMCOG1089: methyltransferase

sfmM2: Methylation

MITE: MITE0000032Methylation (identity: 100.00%)
smcogs: SMCOG1042: O-methyltransferase

sfmM3: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

sfmS4: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Ligases: ligase

sfmA: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

sfmB: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

sfmC: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000422.5	1.03	NRPS	saframycin A, saframycin B	Streptomyces lavendulae
BGC0000910.3	0.51	other (other)	melanin	Streptomyces coelicolor A3(2)
BGC0000911.3	0.49	other (other)	melanin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002135.2	0.43	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.41	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.39	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001050.5	0.39	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0001166.5	0.39	NRPS	HC-toxin	Alternaria jesenskae
BGC0001132.4	0.38	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.38	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000422.5	0.99	NRPS	saframycin A, saframycin B	Streptomyces lavendulae
BGC0000910.3	0.79	other (other)	melanin	Streptomyces coelicolor A3(2)
BGC0000911.3	0.78	other (other)	melanin	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001050.5	0.71	NRPS, PKS	thalassospiramide A	Tistrella bauzanensis
BGC0001166.5	0.71	NRPS	HC-toxin	Alternaria jesenskae
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Similar known clusters from MIBiG 4.0

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 57763:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala) + (Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 4413 - 57763:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ala) + (Gly) + (X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

sfmA: NH2 - Ala

Search NORINE for peptide: strict or relaxed

CAL_domain (124..567): NH2

minowa: NH2

Prediction, score:

NH2: 95.7
Acetyl-CoA: 18.2
fatty_acid: 12.0
shikimic_acid: 0.0
AHBA: 0.0

AMP-binding (1236..1624): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLFNNALTYK
Nearest Stachelhaus code(s):: Ala DLFNNALTYK (100% 8Å match); Gly DLFNNALTYK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

sfmB: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (456..845): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGLIWK
Nearest Stachelhaus code(s):: Gly DILQLGLIWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

sfmC: X

AMP-binding (475..876): X

nrpys: ohdmTyr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DPWGLGLIDK
Nearest Stachelhaus code(s):: ohdmTyr DPWGLGLIDK (100% 8Å match); ohmeTyr DPWGLGLIDK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor