LOCUS       BGC0000427              6461 bp    DNA     linear   BCT 28-JAN-2014
DEFINITION  Anabaena variabilis ATCC 29413, complete genome.
ACCESSION   BGC0000427
VERSION     BGC0000427
KEYWORDS    .
SOURCE      Trichormus variabilis ATCC 29413 (Anabaena flos-aquae UTEX 1444)
  ORGANISM  Trichormus variabilis ATCC 29413
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae;
            Trichormus.
COMMENT     URL -- http://www.jgi.doe.gov
            Source DNA available from Teresa Thiel (thiel@umsl.edu)
            Bacteria available from ATCC: ATCC 29413
            Whole genome sequencing and draft assembly at JGI-PGF
            Finishing done by JGI-LANL
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Contacts: Paul Richardson (microbes@cuba.jgi-psf.org)
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:36:32
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 4798899
            Orig. end    :: 4805360
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..2667)
                     /EC_number="5.1.1.11"
                     /NRPS_PKS="Domain: AMP-binding (33-431). E-value: 9.8e-125.
                     Score: 408.2. Matches aSDomain:
                     nrpspksdomains_Ava_3855_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (540-609). E-value: 9.2e-22. Score:
                     68.9. Matches aSDomain: nrpspksdomains_Ava_3855_PCP.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR000873"
                     /db_xref="InterPro:IPR001031"
                     /db_xref="InterPro:IPR006162"
                     /db_xref="InterPro:IPR006163"
                     /db_xref="InterPro:IPR010071"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     391.2; E-value: 9.6e-119)"
                     /gene_kind="biosynthetic"
                     /locus_tag="Ava_3855"
                     /product="Amino acid adenylation"
                     /protein_id="ABA23460.1"
                     /sec_met_domain="AMP-binding (E-value: 3e-138, bitscore:
                     448.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.2e-15, bitscore:
                     45.2, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQTIDFNIRKLLVEWNATHRDYDLSQSLHELIVAQVERTPEAIAV
                     TFDKQQLTYQELNHKANQLGHYLQTLGVQPETLVGVCLERSLEMVICLLGILKAGGAYV
                     PIDPEYPQERIAYMLEDSQVKVLLTQEKLLNQIPHHQAQTICVDREWEKISTQANTNPK
                     SNIKTDNLAYVIYTSGSTGKPKGAMNTHKGICNRLLWMQEAYQIDSTDSILQKTPFSFD
                     VSVWEFFWTLLTGARLVIAKPGGHKDSAYLIDLITQEQITTLHFVPSMLQVFLQNRHVS
                     KCSSLKRVICSGEALSIDLQNRFFQHLQCELHNLYGPTEAAIDVTFWQCRKDSNLKSVP
                     IGRPIANTQIYILDADLQPVNIGVTGEIYIGGVGVARGYLNKEELTKEKFIINPFPNSE
                     FKRLYKTGDLARYLPDGNIEYLGRTDYQVKIRGYRIEIGEIENVLSSHPQVREAVVIAR
                     DDNAQEKQIIAYITYNSIKPQLDNLRDFLKARLPDFMIPAAFVMLEHLPLTPSGKVDRK
                     ALPKPDLFNYSEHNSYVAPRNEVEEKLVQIWSNILHLPKVGVTENFFAIGGNSLKALHL
                     ISQIEELFAKEISLATLLTNPVIADLAKVIQANNQIHNSPLVPIQPQGKQQPFFCIHPA
                     GGHVLCYFKLAQYIGTDQPFYGLQAQGFYGDEAPLTRVEDMASLYVKTIREFQPQGPYR
                     VGGWSFGGVVAYEVAQQLHRQGQEVSLLAILDSYVPILLDKQKPIDDVYLVGVLSRVFG
                     GMFGQDNLVTPEEIENLTVEEKINYIIDKARSARIFPPGVERQNNRRILDVLVGTLKAT
                     YSYIRQPYPGKVTVFRAREKHIMAPDPTLVWVELFSVMAAQEIKIIDVPGNHYSFVLEP
                     HVQVLAQRLQDCLENNS"
     subregion       1..6461
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="NRPS"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6461
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..2667)
                     /locus_tag="Ava_3855"
     CDS_motif       complement(532..606)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Ava_3855_0007"
                     /evalue="5.50E-16"
                     /label="NRPS-te1"
                     /locus_tag="Ava_3855"
                     /protein_end="712"
                     /protein_start="687"
                     /score="50.7"
                     /tool="antismash"
                     /translation="QPQGPYRVGGWSFGGVVAYEVAQQL"
     CDS_motif       complement(757..786)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Ava_3855_0006"
                     /evalue="3.80E-03"
                     /label="NRPS-beforete1"
                     /locus_tag="Ava_3855"
                     /protein_end="637"
                     /protein_start="627"
                     /score="10.3"
                     /tool="antismash"
                     /translation="PFFCIHPAGG"
     aSDomain        complement(841..1047)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Ava_3855_PCP.1"
                     /evalue="9.20E-22"
                     /label="Ava_3855_PCP.1"
                     /locus_tag="Ava_3855"
                     /protein_end="609"
                     /protein_start="540"
                     /score="68.9"
                     /tool="antismash"
                     /translation="EEKLVQIWSNILHLPKVGVTENFFAIGGNSLKALHLISQIEELFA
                     KEISLATLLTNPVIADLAKVIQAN"
     aSModule        complement(841..2568)
                     /complete
                     /domains="nrpspksdomains_Ava_3855_AMP-binding.1"
                     /domains="nrpspksdomains_Ava_3855_PCP.1"
                     /locus_tags="Ava_3855"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1171..1188)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Ava_3855_0005"
                     /evalue="9.90E+01"
                     /label="NRPS-beforete1"
                     /locus_tag="Ava_3855"
                     /protein_end="499"
                     /protein_start="493"
                     /score="-3.1"
                     /tool="antismash"
                     /translation="FMIPAA"
     CDS_motif       complement(1345..1410)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Ava_3855_0004"
                     /evalue="6.70E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="Ava_3855"
                     /protein_end="441"
                     /protein_start="419"
                     /score="34.2"
                     /tool="antismash"
                     /translation="YLGRTDYQVKIRGYRIEIGEIE"
     aSDomain        complement(1375..2568)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Ava_3855_AMP-binding.1"
                     /evalue="9.80E-125"
                     /label="Ava_3855_AMP-binding.1"
                     /locus_tag="Ava_3855"
                     /protein_end="431"
                     /protein_start="33"
                     /score="408.2"
                     /tool="antismash"
                     /translation="AQVERTPEAIAVTFDKQQLTYQELNHKANQLGHYLQTLGVQPETL
                     VGVCLERSLEMVICLLGILKAGGAYVPIDPEYPQERIAYMLEDSQVKVLLTQEKLLNQI
                     PHHQAQTICVDREWEKISTQANTNPKSNIKTDNLAYVIYTSGSTGKPKGAMNTHKGICN
                     RLLWMQEAYQIDSTDSILQKTPFSFDVSVWEFFWTLLTGARLVIAKPGGHKDSAYLIDL
                     ITQEQITTLHFVPSMLQVFLQNRHVSKCSSLKRVICSGEALSIDLQNRFFQHLQCELHN
                     LYGPTEAAIDVTFWQCRKDSNLKSVPIGRPIANTQIYILDADLQPVNIGVTGEIYIGGV
                     GVARGYLNKEELTKEKFIINPFPNSEFKRLYKTGDLARYLPDGNIEYLGRTDYQVKIR"
     CDS_motif       complement(1498..1584)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Ava_3855_0003"
                     /evalue="1.00E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="Ava_3855"
                     /protein_end="390"
                     /protein_start="361"
                     /score="49.7"
                     /tool="antismash"
                     /translation="IGVTGEIYIGGVGVARGYLNKEELTKEKF"
     CDS_motif       complement(2095..2154)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Ava_3855_0002"
                     /evalue="3.30E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="Ava_3855"
                     /protein_end="191"
                     /protein_start="171"
                     /score="31.8"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGKPKGAMNTH"
     CDS_motif       complement(2347..2388)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_Ava_3855_0001"
                     /evalue="3.30E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="Ava_3855"
                     /protein_end="107"
                     /protein_start="93"
                     /score="20.1"
                     /tool="antismash"
                     /translation="LGILKAGGAYVPID"
     gene            complement(2824..4200)
                     /locus_tag="Ava_3856"
     CDS             complement(2824..4200)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     mycosporine-like: ATP-grasp_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="Ava_3856"
                     /product="ATP-grasp enzyme-like protein"
                     /protein_id="ABA23461.1"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.3e-09, bitscore:
                     26.5, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQSLPLSSAPATPSLPSQTKIAAIIQNICTLALLLLALPINATI
                     VFISLLVFRPQKVKAANPQTILISGGKMTKALQLARSFHAAGHRVVLVETHKYWLTGHR
                     FSQAVDKFYTVPAPQDNPQAYIQALVDIVKQENIDVYIPVTSPVGSYYDSLAKPELSHY
                     CEVFHFDADITQMLDDKFALTQKARSLGLSVPKSFKITSPEQVINFDFSGETRKYILKS
                     IPYDSVRRLDLTKLPCATPEETAAFVRSLPITPEKPWIMQEFIPGKEFCTHSTVRNGEL
                     RLHCCCESSAFQVNYENVNNPQITEWVQHFVKELKLTGQISFDFIQAEDGTVYAIECNP
                     RTHSAITTFYDHPQVAEAYLSQAPTTETIQPLTTSKPTYWTYHEVWRLTGIRSFTQLQR
                     WLGNIWRGTDAIYQPDDPLPFLMVHHWQIPLLLLNNLRRLKGWTRIDFNIGKLVELGGD
                     "
     gene            complement(4215..5054)
                     /locus_tag="Ava_3857"
     CDS             complement(4215..5054)
                     /EC_number="2.1.1.104"
                     /codon_start=1
                     /db_xref="InterPro:IPR000051"
                     /db_xref="InterPro:IPR002935"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     mycosporine-like: Methyltransf_3"
                     /gene_kind="biosynthetic"
                     /locus_tag="Ava_3857"
                     /product="O-methyltransferase, family 3"
                     /protein_id="ABA23462.1"
                     /sec_met_domain="Methyltransf_3 (E-value: 3e-58, bitscore:
                     184.6, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNVIVQPTARPVTPLGILTKQLEAIVQEVKQHPDLPGELIANIH
                     QAWRLAAGIDPYLEECTTPESPELAALAKTTATEAWGEHFHGGTTVRPLEQEMLSGHIE
                     GQTLKMFVHMTKAKKVLEIGMFTGYSALAMAEALPEDGLLVACEVDPYAAEIGQKAFQQ
                     SPHGGKIRVELDAALATLDKLAEAGESFDLVFIDADKKEYVAYFHKLLGSSLLAPDGFI
                     CVDNTLLQGEVYLPAEERSVNGEAIAQFNHTVAIDPRVEQVLLPLRDGLTIIRRIQP"
     gene            complement(5229..6461)
                     /locus_tag="Ava_3858"
     CDS             complement(5229..6461)
                     /EC_number="4.2.3.4"
                     /codon_start=1
                     /db_xref="InterPro:IPR002658"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     mycosporine-like: DHQ_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1183:3-dehydroquinate synthase (Score: 302; E-value:
                     7.9e-92)"
                     /gene_kind="biosynthetic"
                     /locus_tag="Ava_3858"
                     /product="3-dehydroquinate synthase"
                     /protein_id="ABA23463.1"
                     /sec_met_domain="DHQ_synthase (E-value: 7.8e-60, bitscore:
                     190.3, seeds: 76, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSIVQAKFEAKETSFHVEGYEKIEYDLVYVDGIFEIQNSALADVY
                     QGFGRCLAIVDANVSRLYGNQIQAYFQYYGIELRLFPITITEPDKTIQTFERVIDVFAD
                     FKLVRKEPVLVVGGGLITDVVGFACSTYRRSSNYIRIPTTLIGLIDASVAIKVAVNHRK
                     LKNRLGAYHASRKVFLDFSLLRTLPTDQVRNGMAELVKIAVVAHQEVFELLEKYGEELL
                     RTHFGNIDATPEIKEIAHRLTYKAIHKMLELEVPNLHELDLDRVIAYGHTWSPTLELAP
                     RLPMFHGHAVNVDMAFSATIAARRGYITIAERDRILGLMSRVGLSLDHPMLDIDILWRG
                     TESITLTRDGLLRAAMPKPIGDCVFVNDLTREELAAALADHKELCTSYPRGGEGVDVYP
                     VYQKELIGSVK"
ORIGIN
        1 ttatgaatta ttttccagac aatcttgtaa acgctgtgct aaaacctgta catggggttc
       61 tagaacaaac gaataatggt ttccagggac atcaataatc ttaatttctt gagccgccat
      121 tacagaaaat aattctaccc aaactaaggt cgggtcagga gccataatat gtttttccct
      181 ggctcgaaat acagtgactt ttcctggata tggttgtctt atataggaat aagttgcttt
      241 taaagttccc accaaaacat caagaatacg gcgattattt tgacgttcta caccaggcgg
      301 gaatattcta gcgctccgtg ctttatcaat gatgtaatta attttttctt ctacagttaa
      361 attttctatt tcttcaggtg tgactagatt atcttgacca aacataccgc caaaaactct
      421 ggagagaaca ccaactaaat aaacgtcatc aatgggtttt tgtttatcca gcagaatcgg
      481 tacgtaagaa tctaatattg ctagtaaaga tacttcttgt ccttgtctat gtaactgctg
      541 tgctacttca taagctacga ctccaccaaa tgaccacccc ccgacacgat aaggcccttg
      601 gggttgaaat tctctaatag ttttgacgta gagactagcc atatcttcaa ctcgcgtcaa
      661 gggtgcttca tctccataaa atccttgagc ttgtaagcca taaaatggtt ggtcagttcc
      721 tatatattgt gcgagtttaa aatagcataa aacatgacca ccagcaggat gtatacaaaa
      781 gaaaggctgc tgcttacctt gtggttgaat tggaactagg ggtgaattat ggatttggtt
      841 gtttgcttga ataaccttgg ctaaatctgc aattactgga tttgttaaaa gtgttgctaa
      901 ggatatctct ttagcaaata actcttcaat ttgagaaatt aaatgtagag ctttgaggga
      961 attaccacca atagcgaaaa agttttctgt cacacctact ttaggtaaat gcagaatatt
     1021 cgaccagatt tgtactaatt tttcttcaac ttcattccga ggcgctacat aggaattatg
     1081 ttcactataa ttaaataaat caggcttagg taatgcctta cggtctactt taccactggg
     1141 agttaaagga agatgctcca gcatcacaaa agcggctgga atcataaaat caggtagcct
     1201 tgcttttagg aaatcacgca gattatcaag ctgaggtttg atggagttat aggtaatata
     1261 agcgatgatt tgtttttctt gagcgttatc atcccgcgct atgactacag cttctctgac
     1321 ttgtgggtgt gaagataaaa cattttcaat ctcgccaatt tcaattctat aaccccgaat
     1381 ttttacttga taatctgttc taccaagata ttcaatattt ccatcgggta aataacgagc
     1441 taaatcacct gttttataaa gtcgcttaaa ctcagaattg ggaaagggat taataataaa
     1501 tttttctttg gtcaattctt ctttattcaa ataaccacga gcaaccccta caccaccaat
     1561 ataaatttca ccagtgacac caatatttac tggttgtaaa tcggcatcaa gaatataaat
     1621 ttgagtatta gcaatgggac gaccaatagg tacactcttt aaattactat cttttctaca
     1681 ttgccaaaat gtgacatcaa ttgctgcttc tgtcgggcca tagaggttat gtaattcaca
     1741 ttgcaaatgc tggaaaaatc tattttgtaa atctatagat aaagcttcac cgctacaaat
     1801 aactcttttt agagagctgc atttgcttac atggcgattt tgtaaaaaca cttgcagcat
     1861 tgaggggaca aaatgcaacg tagtgatttg ttcttgagta attaaatcga tgaggtaagc
     1921 actatcttta tgtccgcctg gtttggctat taccaaacgt gcgccagtta ataaagtcca
     1981 aaagaactcc caaacggaaa catcaaaact aaagggggtt ttttgtaaaa tgctatctgt
     2041 ggaatcgatt tgataagctt cctgcatcca caataagcga ttacagatac ctttgtgggt
     2101 gttcattgca ccttttggtt taccagtgga accagaggtg taaattacat aagcaagatt
     2161 atccgttttt atattacttt tgggattggt attagcttgt gtggaaattt tctcccattc
     2221 cctatctaca cagatagttt gtgcttgatg gtggggaatt tgattgagta atttttcttg
     2281 agttagtagt accttcacct gagaatcttc tagcatataa gctatgcgtt cttgaggata
     2341 ttcagggtca ataggaacat aagcaccccc agctttgagg attcctaaaa gacagataac
     2401 catttctaag gaacgttcta aacaaacgcc taccagggtt tctggctgga ctcctaatgt
     2461 ttgtaaataa tgtcctagct ggtttgcttt atgatttagt tcttgataag ttagttgttg
     2521 cttgtcaaag gtgacagcga tcgcctcagg tgttcgttct acttgagcta caattagttc
     2581 atgtaaactc tgggaaagat cataatctct gtgggtcgcg ttccactcta caagtaactt
     2641 acgaatatta aaatctatag tctgcatatc ttctaacttt gctcaataat aaaaaatttc
     2701 tcacgcagag acgcagagaa aacacactcc gcgtccctct ctcttgaaaa gtttcctacg
     2761 gagggaaacc ctcctccaga acttttcgct gcgctaacct cagcgtccct ctgcgtttaa
     2821 aaactaatct ccccccaatt ccaccaactt cccaatattg aaatctatcc gcgtccaacc
     2881 tttaagacga cgcaaattat tcaataacag taggggaatt tgccaatgat gtaccatcaa
     2941 aaacggtaag gggtcatctg gctgataaat cgcatcagtc ccgcgccaaa tattccccag
     3001 ccatctttgc aactgggtga aagaacggat accagttaaa cgccaaactt cgtgataagt
     3061 ccaataggta ggcttgcttg tcgttagtgg ttgtatggtt tcagtcgtcg gtgcttgact
     3121 caagtacgct tctgctacct gggggtggtc gtaaaatgtg gtaattgctg aatgtgtgcg
     3181 ggggttacac tcgatggcgt aaactgttcc gtcttcggct tggataaagt caaaggaaat
     3241 ctgtcctgtc agtttcagtt ccttgacaaa atgctgtacc cattcggtaa tttgcgggtt
     3301 atttacattc tcataattaa cttggaaggc tgaagattcg caacagcaat gcagtctgag
     3361 ttccccattc cgaacggtgc tatgggtgca gaattcctta ccggggataa attcctgcat
     3421 aatccacggt ttttcgggag taattggcaa acttctgacg aatgctgctg tttcctctgg
     3481 agtagcacag gggagtttgg ttaagtccaa ccgccgcact gagtcgtagg gaatgctttt
     3541 gaggatgtat ttacgtgtct ctccagaaaa atcgaagttg atgacttgtt ctggtgaggt
     3601 aattttaaag gatttgggta ctgataaacc aagcgatcgc gctttttgtg tcaacgcaaa
     3661 tttatcatcc aacatttggg taatatctgc gtcaaagtga aacacttcgc aataatggga
     3721 taactctggt ttggctaatg agtcgtagta gctacccact ggactggtga cgggaatata
     3781 aacatcgatg ttttcttgtt tgacgatatc taccaaagcc tgaatgtaag cttggggatt
     3841 gtcctggggt gcggggactg tgtaaaactt atccactgct tgggaaaaac gatgaccagt
     3901 caaccagtat ttatgggttt ccaccaagac aactctatgt ccagccgcgt ggaatgacct
     3961 tgctagttgt aaagctttgg tcatcttacc gccactgata agaatggttt gggggtttgc
     4021 tgctttgacc ttttgcggtc ggaagactaa caaggatata aaaacaatgg tggcattaat
     4081 gggcaatgct agtaataaca aagccaaagt gcagatattt tggataattg cggctatttt
     4141 cgtctgggaa ggaagagacg gtgtagcagg tgcggaagaa aggggaaggg attgtgccat
     4201 agtcgattgg acaattaagg ttgtattctg cggataattg ttaaaccatc tcgcaacggc
     4261 aacaaaacct gttctacacg ggggtctata gctactgtat gattaaattg cgcgatcgct
     4321 tcaccattga cgctacgttc ctctgctggt agataaactt ccccttgtaa taaggtgtta
     4381 tctacacaaa taaagccatc tggtgctaac aaactgctac ctagcaactt gtgaaaatag
     4441 gctacatact cttttttatc tgcgtcgata aataccaagt caaaagactc cccagcttct
     4501 gctaacttat caagagttgc taaggctgca tccaattcca cacgaatctt tccaccgtgg
     4561 ggagattgtt gaaaggcttt ctgtccaatt tccgccgcgt aagggtcaac ttcacaagcc
     4621 acaagcagtc catcctctgg taatgcttcc gccatcgcca gcgccgaata accggtaaac
     4681 atcccaattt ctaagacttt tttagctttg gtcatgtgaa caaacatctt taaggtttgt
     4741 ccttcgatat gaccagaaag catctcttgt tctagaggac ggacggttgt acctccgtgg
     4801 aagtgttctc cccaggcttc ggtggctgtg gtttttgcca atgcagcgag ttcaggagat
     4861 tctggagtgg tgcattcttc caaataaggg tctatacctg cggctaaacg ccaagcctga
     4921 tggatgtttg ctatcaattc cccaggtaaa tctggatgtt gcttaacctc ttggactatg
     4981 gcttctaact gcttggttaa aattcccaat ggtgtaacag gtctagctgt tggttggaca
     5041 atcacatttg tcaagtcgct tcgctccaat tcaaaattca aaattcaaaa ttcaaaatta
     5101 aagacaatta gtgtccgatt atttgcgtag ccttctcttt ccctacggga cgctccgcga
     5161 acagaaatgc taccgcgctc gcgcagtgta tccgtggagt attttgcatt ttgaattcaa
     5221 aaaagtcatt atttaacact cccgattaat tctttttgat aaacgggata cacatccaca
     5281 ccttcaccac cacggggata actggtacaa agttctttgt ggtcagctaa tgcggctgct
     5341 aattcttctc ttgtcaggtc attgacgaag acacaatcac caatgggttt tggcatagca
     5401 gctcttaaca aaccatcacg agttaatgtg atagattcag taccacgcca caaaatatct
     5461 atatccaaca tgggatggtc gagggataga ccaacgcgac tcattaatcc taaaatacga
     5521 tcgcgttctg caattgtaat atatcctcta cgggcggcga tcgttgccga gaaagccata
     5581 tctacattaa cggcgtgtcc gtggaacatg ggtagacgag gcgcaagttc caaggtggga
     5641 ctccaagtgt gaccgtaagc aatcacccta tctaggtcta actcatgcag gttgggaact
     5701 tccaattcca acatcttatg gatagctttg taagtcaaac gatgggctat ttctttaatc
     5761 tctggagttg catctatatt gccaaaatga gtacgtagta attcttcgcc gtacttctcc
     5821 aacaattcaa aaacttcttg atgcgctact acagcgattt ttaccaattc cgccatcccg
     5881 ttacgtactt ggtctgtagg gagagtacgc aacaaggaga aatctaaaaa tactttgcga
     5941 gaagcatgat aagcacccaa acggtttttc agtttgcgat gattaactgc taccttaatt
     6001 gctacactgg catcaattaa tccaatcaat gtagtaggaa tgcggatgta attgctgctg
     6061 cgacgatatg tagaacaagc aaagccgaca acatctgtaa ttaaaccgcc acccacgact
     6121 aatactggtt ctttgcggac taatttgaaa tctgcaaaga catctataac tctctcgaaa
     6181 gtttgaatag tcttatctgg ttcagtaatg gtaataggaa atagcctcag ttctatacca
     6241 taatactgga aatatgcctg aatttgatta ccatacaacc gactgacgtt agcatctaca
     6301 atcgccaagc atcgtccaaa accttgatat acatctgcta gtgcagaatt ctggatttca
     6361 aaaataccat ctacatacac caaatcatac tcaatctttt cgtaaccttc tacatgaaaa
     6421 gatgtttcct tagcttcaaa ctttgcttgg acgatactca t
//
