BGC0000429: skyllamycin A biosynthetic gene cluster from Streptomyces sp. Acta 2897
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Location: 1 - 86,806 nt. (total: 86,806 nt).
This entry is originally from NCBI GenBank JF430460.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
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resistance
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Gene details
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General
Compounds
Genes
Biosynthesis
History
KnownClusterBlast
NRPS/PKS domains
General information about the BGC
MIBiG accession BGC0000429
Short description skyllamycin A biosynthetic gene cluster from Streptomyces sp. Acta 2897
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Unknown)
Loci
JF430460.1
via Heterologous expression
Compounds
  • skyllamycin A
  • skyllamycin B
Species Streptomyces sp. Acta 2897 [taxonomy]
References
Chemical products information
skyllamycin A Evidence:
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C75H94N12O20
skyllamycin B Evidence:
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C74H92N12O20
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AEA30245.1
  • sky1
 148 - 561 (-) putative SMR-type multi-drug efflux transporter
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    • AEA30244.1
    • sky2
    		 662 - 982 (-) putative SMR-type multi-drug efflux transporter
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      • AEA30246.1
      		 1093 - 1572 (-) putative cytosine/adenosine deaminase
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        • AEA30247.1
        • sky4
        		 1723 - 2841 (-) putative oxidoreductase
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          • AEA30248.1
          • sky5
          		 2843 - 3499 (-) putative isomerase
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            • AEA30249.1
            • sky6
            		 3724 - 4341 (+) putative N-acetyltransferase
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              • AEA30250.1
              		 4330 - 4737 (-) hypothetical protein
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                • AEA30251.1
                • sky8
                		 4894 - 5730 (+) putative DNA-binding protein
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                  • AEA30252.1
                  		 5727 - 5963 (+) hypothetical protein
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                    • AEA30253.1
                    		 6119 - 7495 (+) putative integrin-like protein
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                      • AEA30254.1
                      • sky11
                      		 7755 - 9338 (-) putative carboxyltransferase
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                        • AEA30255.1
                        		 9332 - 10375 (-) putative isoprenyl diphosphate synthase
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                          • AEA30256.1
                          • sky13
                          		 11015 - 11932 (+) putative ACP-acyltransferase
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                            • AEA30257.1
                            • sky14
                            		 11985 - 13949 (+) putative ABC1-family protein kinase
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                              • AEA30258.1
                              • sky15
                              		 14040 - 14261 (-) putative MbtH-like protein
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                                • AEA30259.1
                                • sky16
                                		 14696 - 14950 (+) putative acyl carrier protein
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                                  • AEA30260.1
                                  • sky17
                                  		 15003 - 16259 (+) putative 3-oxoacyl-ACP synthase II
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                                    • AEA30261.1
                                    • sky18
                                    		 16256 - 17389 (+) putative 3-oxoacyl-ACP synthase II
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                                      • AEA30262.1
                                      • sky19
                                      		 17386 - 18348 (+) putative 3-oxoacyl-ACP synthase I
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                                        • AEA30263.1
                                        • sky20
                                        		 18393 - 19211 (+) putative hydrolase
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                                          • AEA30264.1
                                          • sky21
                                          		 19217 - 20257 (+) putative thioesterase
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                                            • AEA30265.1
                                            • sky22
                                            		 20286 - 21389 (+) putative 3-oxoacyl-ACP synthase I
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                                              • AEA30266.1
                                              • sky23
                                              		 21449 - 21697 (+) putative acyl carrier protein
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                                                • AEA30267.1
                                                • sky24
                                                		 21751 - 22140 (+) putative dehydratase
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                                                  • AEA30268.1
                                                  • sky25
                                                  		 22137 - 22625 (+) putative 3-hydroxyacyl-ACP dehydratase
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                                                    • AEA30269.1
                                                    • sky26
                                                    		 22647 - 23393 (+) putative 3-oxoacyl-ACP reductase
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                                                      • AEA30270.1
                                                      • sky27
                                                      		 23435 - 24145 (+) putative isomerase
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                                                        • AEA30271.1
                                                        • sky28
                                                        		 24243 - 25910 (+) putative oxidoreductase
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                                                          • AEA30272.1
                                                          • sky29
                                                          		 26089 - 40332 (+) peptide synthetase
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                                                            • AEA30273.1
                                                            • sky30
                                                            		 40329 - 54515 (+) peptide synthetase
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                                                              • AEA30274.1
                                                              • sky31
                                                              		 54509 - 66241 (+) peptide synthetase
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                                                                • AEA30275.1
                                                                • sky32
                                                                		 66281 - 67552 (+) P450 monooxygenase
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                                                                  • AEA30276.1
                                                                  • sky33
                                                                  		 67549 - 68322 (+) putative type II thioesterase
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                                                                    • AEA30277.1
                                                                    		 68342 - 68863 (-) hypothetical protein
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                                                                      • AEA30278.1
                                                                      • sky35
                                                                      		 69263 - 70240 (+) putative ABC transporter
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                                                                        • AEA30279.1
                                                                        • sky36
                                                                        		 70237 - 71025 (+) putative ABC transporter
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                                                                          • AEA30280.1
                                                                          • sky37
                                                                          		 71195 - 72223 (+) O-methyltransferase
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                                                                            • AEA30281.1
                                                                            • sky38
                                                                            		 72237 - 73031 (+) putative ABC transporter
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                                                                              • AEA30282.1
                                                                              • sky39
                                                                              		 73116 - 74186 (+) flavin-dependent monooxygenase
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                                                                                • AEA30283.1
                                                                                • sky40
                                                                                		 74223 - 74789 (+) putative flavin reductase
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                                                                                  • AEA30284.1
                                                                                  • sky41
                                                                                  		 74880 - 76196 (-) glutamate mutase subunit B
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                                                                                    • AEA30285.1
                                                                                    • sky42
                                                                                    		 76193 - 76663 (-) glutamate mutase subunit A
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                                                                                      • AEA30286.1
                                                                                      • sky43
                                                                                      		 77324 - 78055 (-) putative IclR-family transcriptional regulator
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                                                                                        • AEA30287.1
                                                                                        • sky44
                                                                                        		 78748 - 80688 (-) putative AfsR-like transcriptional regulator
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                                                                                          • AEA30288.1
                                                                                          • sky45
                                                                                          		 80717 - 81394 (-) putative IclR-family transcriptional regulator
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                                                                                            • AEA30289.1
                                                                                            • sky46
                                                                                            		 81812 - 82651 (-) putative LuxR-family transcriptional regulator
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                                                                                              • AEA30290.1
                                                                                              • sky47
                                                                                              		 82799 - 83590 (-) putative LuxR-family transcriptional regulator
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                                                                                                • AEA30291.1
                                                                                                • sky48
                                                                                                		 84713 - 85858 (-) putative DNA-binding protein
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                                                                                                  • AEA30292.1
                                                                                                  		 86556 - >86806 (+) putative DNA polymerase III
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                                                                                                    Biosynthesis information

                                                                                                    Biosynthetic modules

                                                                                                    Name
                                                                                                    1
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky29
                                                                                                    Substrates
                                                                                                    threonine (evidence: Structure-based inference)
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation (Starter), adenylation
                                                                                                    Name
                                                                                                    2
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky29
                                                                                                    Substrates
                                                                                                    alanine (evidence: Structure-based inference)
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation (LCL), adenylation
                                                                                                    Name
                                                                                                    3
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky29
                                                                                                    Substrates
                                                                                                    aspartic acid, 3S-methylaspartic acid (evidence: Structure-based inference)
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation (LCL), adenylation
                                                                                                    Name
                                                                                                    4
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky29
                                                                                                    Substrates
                                                                                                    glycine (evidence: Structure-based inference)
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation (LCL), adenylation, epimerase
                                                                                                    Name
                                                                                                    5
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky30
                                                                                                    Substrates
                                                                                                    phenylalanine (evidence: Structure-based inference, Knock-out studies)
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation, adenylation, oxidase
                                                                                                    Name
                                                                                                    6
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky30
                                                                                                    Substrates
                                                                                                    proline (evidence: Structure-based inference)
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation (LCL), adenylation
                                                                                                    Name
                                                                                                    7
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky30
                                                                                                    Substrates
                                                                                                    O-methyltyrosine, phenylalanine (evidence: Structure-based inference, Knock-out studies)
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation (LCL), adenylation, oxidase
                                                                                                    Name
                                                                                                    8
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky30
                                                                                                    Substrates
                                                                                                    tryptophan (evidence: Structure-based inference [1])
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation (LCL), adenylation, epimerase
                                                                                                    Name
                                                                                                    10
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky31
                                                                                                    Substrates
                                                                                                    leucine (evidence: Structure-based inference)
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation (LCL), adenylation, epimerase
                                                                                                    Name
                                                                                                    11
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky31
                                                                                                    Substrates
                                                                                                    leucine (evidence: Structure-based inference)
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation, adenylation, oxidase
                                                                                                    Name
                                                                                                    9
                                                                                                    Type
                                                                                                    nrps-type1
                                                                                                    Genes
                                                                                                    sky31
                                                                                                    Substrates
                                                                                                    glycine (evidence: Structure-based inference)
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    condensation, adenylation
                                                                                                    Name
                                                                                                    None
                                                                                                    Type
                                                                                                    pks-modular-starter
                                                                                                    Genes
                                                                                                    sky13
                                                                                                    Substrates
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    acyltransferase
                                                                                                    Name
                                                                                                    None
                                                                                                    Type
                                                                                                    pks-trans-at
                                                                                                    Genes
                                                                                                    sky17
                                                                                                    Substrates
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    ketosynthase
                                                                                                    Name
                                                                                                    None
                                                                                                    Type
                                                                                                    pks-trans-at
                                                                                                    Genes
                                                                                                    sky18
                                                                                                    Substrates
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    ketosynthase
                                                                                                    Name
                                                                                                    None
                                                                                                    Type
                                                                                                    pks-trans-at
                                                                                                    Genes
                                                                                                    sky19
                                                                                                    Substrates
                                                                                                    Integrated Monomers
                                                                                                    Domains
                                                                                                    ketosynthase
                                                                                                    Annotation changelog

                                                                                                    Entry version: 4

                                                                                                    Date
                                                                                                    Changes
                                                                                                    Submitters
                                                                                                    Reviewers
                                                                                                    2024-07-24
                                                                                                    MIBiG v4 annotathon
                                                                                                    • (ID: C62OJVVH7XOTOTFZSJT727YU)
                                                                                                    • (ID: X3QVFXOIMTNXSB4JQGP4RI6A)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                                                    Entry version: 3

                                                                                                    Date
                                                                                                    Changes
                                                                                                    Submitters
                                                                                                    Reviewers
                                                                                                    2022-09-15
                                                                                                    Manually updates compound information for skyllamycin A & B (Structure from Pubchem isn't correct)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    2022-09-15
                                                                                                    Corrected NRP module activity
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    2022-09-15
                                                                                                    Removed gene names of 'No gene ID'
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    2022-09-15
                                                                                                    Removed duplicate gene names
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    2022-09-15
                                                                                                    Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    2022-09-15
                                                                                                    Removed module numbers from incomplete PKS modules
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    2022-09-15
                                                                                                    Updated NRP substrate specificities
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                                                    Entry version: 2

                                                                                                    Date
                                                                                                    Changes
                                                                                                    Submitters
                                                                                                    Reviewers
                                                                                                    2019-10-16
                                                                                                    Migrated from v1.4
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    2019-10-16
                                                                                                    Updated compound(s) information (MIBiG Annotathon)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    2019-10-16
                                                                                                    Updated compound(s) information (NPAtlas curation)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                                                    Entry version: 1

                                                                                                    Date
                                                                                                    Changes
                                                                                                    Submitters
                                                                                                    Reviewers
                                                                                                    2015-06-12
                                                                                                    Submitted
                                                                                                    • (ID: M2JBIQNJAZIP5YVYS7CZLALR)
                                                                                                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                                                    Similar known clusters from MIBiG 4.0
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                                                                                                    Location:

                                                                                                    Identifier
                                                                                                    Identity
                                                                                                    Coverage
                                                                                                    Detailed domain annotation
                                                                                                    Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
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