Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000429.1:
Cluster 1NRP186806

BGC0000429, skyllamycin biosynthetic gene cluster from Streptomyces sp. Acta. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Skyllamycin A
Exact molecular mass: 1505.6598 Da ([M+Na]+)
Molecular activity: Inhibitor
Molecular target: Platelet-derived growth factor (PDGF)

Class-specific details

Biosynthetic class(es):
NRP
Cyclic depsipeptide (cyclic)
Thioesterase type:
Unknown
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
sky29 (AEA30272.1)
Module 1
A specificity: Threonine
Evidence for specificity: Structure-based inference
C domain subtype: Starter
Module 2
A specificity: Alanine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 3
A specificity: Beta-methoxyaspartate
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 4
A specificity: Glycine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: LCL
sky30 (AEA30273.1)
Module 5
A specificity: Phenylalanine
Evidence for specificity: Structure-based inference
C domain subtype: Other
Scaffold-modifying domain: Oxidation
Module 6
A specificity: Proline
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 7
A specificity: Tyrosine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Scaffold-modifying domain: Oxidation
Module 8
A specificity: D-tryptophan
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: LCL
sky31 (AEA30274.1)
Module 10
A specificity: D-leucine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: LCL
Module 11
A specificity: D-leucine
Evidence for specificity: Structure-based inference
C domain subtype: Other
Scaffold-modifying domain: Oxidation
Module 9
A specificity: Glycine
Evidence for specificity: Structure-based inference
C domain subtype: Other

Gene cluster description

skyllamycin (BGC0000429). Gene Cluster 1. Biosynthetic class = NRP. GenBank JF430460. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Heterologous expression
MIxS-compliance:Unknown
Contact for this cluster:Daniel Petras (TU Berlin / AK Suessmuth)

Literature references

1. Pohle S et al. (2011) Biosynthetic gene cluster of the non-ribosomally synthesized cyclodepsipeptide skyllamycin: deciphering unprecedented ways of unusual hydroxylation reactions. J Am Chem Soc 133(16):6194-205. doi: 10.1021/ja108971p. Epub 2011
2. Toki S et al. (2001) RP-1776, a novel cyclic peptide produced by Streptomyces sp., inhibits the binding of PDGF to the extracellular domain of its receptor. J Antibiot (Tokyo) 54(5):405-14.