BGC0000429: skyllamycin A biosynthetic gene cluster from Streptomyces sp. Acta 2897
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Location: 1 - 86,806 nt. (total: 86,806 nt).
This entry is originally from NCBI GenBank JF430460.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
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resistance
TTA codons
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Gene details
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General
Compounds
Genes
Polyketide
NRP
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000429
Short description skyllamycin A biosynthetic gene cluster from Streptomyces sp. Acta 2897
Status Minimal annotation: no
A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s)

Completeness: complete
Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRP (Cyclic depsipeptide)
  • Polyketide
Loci NCBI GenBank: JF430460.1
Compounds
  • skyllamycin A
  • skyllamycin B
Species Streptomyces sp. Acta 2897 [taxonomy]
References
Chemical products information
skyllamycin A
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C75H94N12O20
skyllamycin B
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C74H92N12O20
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AEA30245.1
  • sky1
 148 - 561 (-) putative SMR-type multi-drug efflux transporter
  • Transport
Sequence-based prediction
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  • AEA30244.1
  • sky2
 662 - 982 (-) putative SMR-type multi-drug efflux transporter
  • Transport
Sequence-based prediction
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  • AEA30246.1
 1093 - 1572 (-) putative cytosine/adenosine deaminase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30247.1
  • sky4
 1723 - 2841 (-) putative oxidoreductase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30248.1
  • sky5
 2843 - 3499 (-) putative isomerase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30249.1
  • sky6
 3724 - 4341 (+) putative N-acetyltransferase
  • Unknown
Sequence-based prediction
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  • AEA30250.1
 4330 - 4737 (-) hypothetical protein
  • Unknown
Sequence-based prediction
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  • AEA30251.1
  • sky8
 4894 - 5730 (+) putative DNA-binding protein
  • Unknown
Sequence-based prediction
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  • AEA30252.1
 5727 - 5963 (+) hypothetical protein
  • Unknown
Sequence-based prediction
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  • AEA30253.1
 6119 - 7495 (+) putative integrin-like protein
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30254.1
  • sky11
 7755 - 9338 (-) putative carboxyltransferase
  • Unknown
Sequence-based prediction
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  • AEA30255.1
 9332 - 10375 (-) putative isoprenyl diphosphate synthase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30256.1
  • sky13
 11015 - 11932 (+) putative ACP-acyltransferase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30257.1
  • sky14
 11985 - 13949 (+) putative ABC1-family protein kinase
  • Unknown
Sequence-based prediction
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  • AEA30258.1
  • sky15
 14040 - 14261 (-) putative MbtH-like protein
  • Unknown
Sequence-based prediction
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  • AEA30259.1
  • sky16
 14696 - 14950 (+) putative acyl carrier protein
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30260.1
  • sky17
 15003 - 16259 (+) putative 3-oxoacyl-ACP synthase II
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30261.1
  • sky18
 16256 - 17389 (+) putative 3-oxoacyl-ACP synthase II
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30262.1
  • sky19
 17386 - 18348 (+) putative 3-oxoacyl-ACP synthase I
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30263.1
  • sky20
 18393 - 19211 (+) putative hydrolase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30264.1
  • sky21
 19217 - 20257 (+) putative thioesterase
  • Unknown
Sequence-based prediction
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  • AEA30265.1
  • sky22
 20286 - 21389 (+) putative 3-oxoacyl-ACP synthase I
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30266.1
  • sky23
 21449 - 21697 (+) putative acyl carrier protein
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30267.1
  • sky24
 21751 - 22140 (+) putative dehydratase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30268.1
  • sky25
 22137 - 22625 (+) putative 3-hydroxyacyl-ACP dehydratase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30269.1
  • sky26
 22647 - 23393 (+) putative 3-oxoacyl-ACP reductase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30270.1
  • sky27
 23435 - 24145 (+) putative isomerase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30271.1
  • sky28
 24243 - 25910 (+) putative oxidoreductase
  • Precursor biosynthesis
Sequence-based prediction
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  • AEA30272.1
  • sky29
 26089 - 40332 (+) peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AEA30273.1
  • sky30
 40329 - 54515 (+) peptide synthetase
  • Scaffold biosynthesis
Sequence-based prediction
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  • AEA30274.1
  • sky31
 54509 - 66241 (+) peptide synthetase
  • Scaffold biosynthesis
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  • AEA30275.1
  • sky32
 66281 - 67552 (+) P450 monooxygenase
  • Tailoring (Hydroxylation)
Activity assay
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  • AEA30276.1
  • sky33
 67549 - 68322 (+) putative type II thioesterase
  • Tailoring (Unknown)
Sequence-based prediction
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  • AEA30277.1
 68342 - 68863 (-) hypothetical protein
  • Unknown
Sequence-based prediction
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  • AEA30278.1
  • sky35
 69263 - 70240 (+) putative ABC transporter
  • Transport
Sequence-based prediction
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  • AEA30279.1
  • sky36
 70237 - 71025 (+) putative ABC transporter
  • Transport
Sequence-based prediction
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  • AEA30280.1
  • sky37
 71195 - 72223 (+) O-methyltransferase
  • Tailoring (Methylation)
Sequence-based prediction
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  • AEA30281.1
  • sky38
 72237 - 73031 (+) putative ABC transporter
  • Transport
Sequence-based prediction
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  • AEA30282.1
  • sky39
 73116 - 74186 (+) flavin-dependent monooxygenase
  • Tailoring (Hydroxylation)
Sequence-based prediction
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  • AEA30283.1
  • sky40
 74223 - 74789 (+) putative flavin reductase
  • Tailoring (Reduction)
Sequence-based prediction
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  • AEA30284.1
  • sky41
 74880 - 76196 (-) glutamate mutase subunit B
  • Tailoring (Unknown)
Sequence-based prediction
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  • AEA30285.1
  • sky42
 76193 - 76663 (-) glutamate mutase subunit A
  • Tailoring (Unknown)
Sequence-based prediction
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  • AEA30286.1
  • sky43
 77324 - 78055 (-) putative IclR-family transcriptional regulator
  • Regulation
Sequence-based prediction
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  • AEA30287.1
  • sky44
 78748 - 80688 (-) putative AfsR-like transcriptional regulator
  • Regulation
Sequence-based prediction
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  • AEA30288.1
  • sky45
 80717 - 81394 (-) putative IclR-family transcriptional regulator
  • Regulation
Sequence-based prediction
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  • AEA30289.1
  • sky46
 81812 - 82651 (-) putative LuxR-family transcriptional regulator
  • Regulation
Sequence-based prediction
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  • AEA30290.1
  • sky47
 82799 - 83590 (-) putative LuxR-family transcriptional regulator
  • Regulation
Sequence-based prediction
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  • AEA30291.1
  • sky48
 84713 - 85858 (-) putative DNA-binding protein
  • Regulation
Sequence-based prediction
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  • AEA30292.1
 86556 - >86806 (+) putative DNA polymerase III
  • Unknown
Sequence-based prediction
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Polyketide-specific information
Subclass Unknown
Cyclic? no
Polyketide-synthases
Genes Properties Modules
sky19
+
sky18
+
sky13
+
sky17 		Synthase subclass:
Module ?
Genes: sky13
Core domains: Acyltransferase
Module ?
Genes: sky17
Core domains: Ketosynthase
Module ?
Genes: sky18
Core domains: Ketosynthase
Module ?
Genes: sky19
Core domains: Ketosynthase
NRP-specific information
Subclass Cyclic depsipeptide
Cyclic? yes
Release type
  • Macrolactonization
Thioesterase genes
  • sky31 (Unknown)
NRP-synthases
Gene Modules
sky29
Module 1
Specificity: threonine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Starter
Module 2
Specificity: alanine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 3
Specificity: aspartic acid / 3S-methylaspartic acid
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 4
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
sky30
Module 5
Specificity: phenylalanine
Evidence for specificity:
  • Structure-based inference
  • Knock-out studies
Condensation domain type: Unknown
Modification domains: Oxidation
Module 6
Specificity: proline
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 7
Specificity: O-methyltyrosine / phenylalanine
Evidence for specificity:
  • Structure-based inference
  • Knock-out studies
Condensation domain type: LCL
Modification domains: Oxidation
Module 8
Specificity: tryptophan
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
sky31
Module 10
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: LCL
Module 11
Specificity: leucine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Unknown
Modification domains: Oxidation
Module 9
Specificity: glycine
Evidence for specificity:
  • Structure-based inference
Condensation domain type: Unknown
Annotation changelog
MIBiG version Submitter Notes
1.0
  • Hidden contributor (ID: M2JBIQNJAZIP5YVYS7CZLALR, no GDPR consent given).
  • Submitted
2.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Migrated from v1.4
  • Updated compound(s) information (MIBiG Annotathon)
  • Updated compound(s) information (NPAtlas curation)
3.0
  • Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).
  • Manually updates compound information for skyllamycin A & B (Structure from Pubchem isn't correct)
  • Corrected NRP module activity
  • Removed gene names of 'No gene ID'
  • Removed duplicate gene names
  • Removed ketoreductase stereochemistry annotation from modules without ketoreductases
  • Removed module numbers from incomplete PKS modules
  • Updated NRP substrate specificities
Detailed domain annotation
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