Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000433.1:
Cluster 1NRP141884

BGC0000433, surfactin biosynthetic gene cluster from Bacillus amyloliquefaciens. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: surfactin
ChemSpider ID: 391754
SMILES string: Copy to clipboard
Molecular formula:
Average molecular mass: Da
Molecular activity: Surfactant

Class-specific details

Biosynthetic class(es):
NRP
Other lipopeptide (cyclic)
Thioesterase type:
None
Release / cyclization type:
None

Nonribosomal peptide synthetases:
srfAA (YP001419995)
Module 1
A specificity: Glutamate
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 2
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 3
A specificity: Leucine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: DCL
srfAB (YP001419996)
Module 4
A specificity: Valine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 5
A specificity: Aspartate
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 6
A specificity: Leucine
Evidence for specificity: Structure-based inference
AA is epimerized
C domain subtype: DCL
srfAC (YP001419998)
Module 7
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Scaffold-modifying domain: Other

Gene cluster description

surfactin (BGC0000433). Gene Cluster 1. Biosynthetic class = NRP. GenBank AJ575642. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies
MIxS-compliance:Unknown
Contact for this cluster:Rainer Borriss (Humboldt University Berlin)

Literature references

1. Koumoutsi A et al. (2004) Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42. J Bacteriol 186(4):1084-96.