LOCUS       BGC0000442              9759 bp    DNA     linear   PLN 27-FEB-2015
DEFINITION  TPA_asm: Aspergillus nidulans FGSC A4 chromosome V.
ACCESSION   BGC0000442
VERSION     BGC0000442
KEYWORDS    .
SOURCE      Aspergillus nidulans FGSC A4
  ORGANISM  Aspergillus nidulans FGSC A4
            Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
            Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae;
            Aspergillus; Aspergillus subgen. Nidulantes.
COMMENT     Aspergillus nidulans FGSC A4 CADRE,
            updated by Eurofungbase with contributions from
            CADRE (http://www.cadre-genomes.org.uk),
            AspGD (http://www.aspergillusgenome.org) and
            Ensembl Genomes (http://www.ensemblgenomes.org).
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:38:55
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 9759
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(join(1..213,277..2913))
                     /NRPS_PKS="Domain: AMP-binding (32-462). E-value: 1.7e-59.
                     Score: 193.3. Matches aSDomain:
                     nrpspksdomains_ANIA_08513_AMP-binding.1"
                     /NRPS_PKS="Domain: PP-binding (599-666). E-value: 3e-07.
                     Score: 22.8. Matches aSDomain:
                     nrpspksdomains_ANIA_08513_PP-binding.1"
                     /NRPS_PKS="Domain: Thioesterase (699-940). E-value:
                     2.4e-29. Score: 95.2. Matches aSDomain:
                     nrpspksdomains_ANIA_08513_Thioesterase.1"
                     /codon_start=1
                     /db_xref="GOA:Q5AT67"
                     /db_xref="InterPro:IPR000873"
                     /db_xref="InterPro:IPR001031"
                     /db_xref="InterPro:IPR009081"
                     /db_xref="InterPro:IPR020845"
                     /db_xref="InterPro:IPR029058"
                     /db_xref="UniProtKB/TrEMBL:Q5AT67"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002:AMP-dependent synthetase and ligase (Score:
                     230.8; E-value: 4e-70)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ANIA_08513"
                     /note="transcript_id=CADANIAT00003005"
                     /old_locus_tag="AN8513.4"
                     /product="TdiA [Source:UniProtKB/TrEMBL;Acc:A7XRY0]"
                     /protein_id="CBF80711.1"
                     /sec_met_domain="AMP-binding (E-value: 5.9e-64, bitscore:
                     204.4, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.7e-08, bitscore:
                     22.8, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPSKTEIAPLRAAKYPFGNIVDALRHAAAHTDEGIIVYHPNSIS
                     TSSPPQTVSYKDLLHQAEANATRLLQQKLCSPKSIVLVHFESALDSIVWYWSVLLAGGI
                     PALTGPGMFSQNPADRERHLRHLSETLNSPVCLTRPALLAPFEEQTADDRIKARTVDEI
                     LAAPEIADVADAPLPALTPSSTDMLALMLTSGSSGNAKAVPLTHQQLLAAFRGKSTAAS
                     LRFPRSPFLSWVHMDHVANLVHCHIFAIVSGISQIQVPAPDLLINPAQLLNLISRHRVS
                     RTFMPNFLCAKLRRQLESGSPEYILDPGLNLETLYIDTGGEANVTEVCIALQSLLSRYG
                     APDNVFKPSFGMTETVAGCIFNSHCPSYDHAQRHEFACLGKPMPGVRMRVTRLDTPSEE
                     AAPGERGSLEVTGEVVFKGYYNNPAATAEAFTSDGWFRTGDLAFIDSNGNLHLDGRTKE
                     MININGVKYLPYELDAALEQAQIPGATPSYFCTFSSRDATMDTEVVVVLYLPSYVESDD
                     EARFSTQSSIIRVVAMHTRSRPRVVPLRPQDMPKSTLGKLSRAKLKTALEEGQFATQQQ
                     INDEAIRRYQQKTRASPETPDEAVILDIIKEQLEIRSDDDSFGVNDSILSIGATSMDLV
                     AIIHRINKCLQPSQPLRLTDILKDSTARGLAVALATGAAPRSQDQSSTHVYDPVVTLQP
                     HGTKSPLWLVHPGVGEVLVFVNLAHHITDRPVYAFRAKGFNAAAGLPETPFTSLEELFT
                     TYRDAIKERQPHGPYAIAGYSFGGMVAFEVSKLLEQDGDEVRYCGSWNLPPHIKFRMRE
                     LVWEECVIHLFYFVGLMTELAAYTHKPTLQEFNRANRRLDAIRYLRQHCDAARWDELGL
                     SEEYYLLWCMDVFVADPLSHVAKDRIDWVEGRLSAWKEFVREDVRFHDVQGAHYTMLNR
                     EYVEAFAGTLKNVLRERGL"
     protocluster    1..9759
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[3302:4695]"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3303..4695
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(indsynth or dmat or indole_PTase)"
                     /neighbourhood="10000"
                     /product="indole"
                     /protocluster_number="1"
     protocluster    1..9759
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:2913]"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      1..2913
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="2"
     cand_cluster    1..9759
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(indsynth or dmat or indole_PTase)"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX))"
                     /kind="neighbouring"
                     /product="indole"
                     /product="NRPS-like"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     subregion       1..9759
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000442"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..9759
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="indole"
                     /product="NRPS-like"
                     /region_number="1"
                     /rules="(indsynth or dmat or indole_PTase)"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..2913)
                     /locus_tag="ANIA_08513"
                     /old_locus_tag="AN8513.4"
     mRNA            complement(join(1..213,277..2913))
                     /locus_tag="ANIA_08513"
                     /note="transcript_id=CADANIAT00003005"
                     /old_locus_tag="AN8513.4"
     aSDomain        complement(join(31..213,277..816))
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_08513_Thioesterase.1"
                     /evalue="2.40E-29"
                     /label="ANIA_08513_Thioesterase.1"
                     /locus_tag="ANIA_08513"
                     /protein_end="940"
                     /protein_start="699"
                     /score="95.2"
                     /tool="antismash"
                     /translation="PLWLVHPGVGEVLVFVNLAHHITDRPVYAFRAKGFNAAAGLPETP
                     FTSLEELFTTYRDAIKERQPHGPYAIAGYSFGGMVAFEVSKLLEQDGDEVRYCGSWNLP
                     PHIKFRMRELVWEECVIHLFYFVGLMTELAAYTHKPTLQEFNRANRRLDAIRYLRQHCD
                     AARWDELGLSEEYYLLWCMDVFVADPLSHVAKDRIDWVEGRLSAWKEFVREDVRFHDVQ
                     GAHYTMLNREYVEAFAGTL"
     aSModule        complement(31..2817)
                     /complete
                     /domains="nrpspksdomains_ANIA_08513_AMP-binding.1"
                     /domains="nrpspksdomains_ANIA_08513_PP-binding.1"
                     /domains="nrpspksdomains_ANIA_08513_Thioesterase.1"
                     /final_module
                     /locus_tags="ANIA_08513"
                     /monomer_pairings="X -> X"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(553..627)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_08513_0003"
                     /evalue="3.20E-12"
                     /label="NRPS-te1"
                     /locus_tag="ANIA_08513"
                     /protein_end="787"
                     /protein_start="762"
                     /score="38.7"
                     /tool="antismash"
                     /translation="QPHGPYAIAGYSFGGMVAFEVSKLL"
     aSDomain        complement(916..1116)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_08513_PP-binding.1"
                     /evalue="3.00E-07"
                     /label="ANIA_08513_PP-binding.1"
                     /locus_tag="ANIA_08513"
                     /protein_end="666"
                     /protein_start="599"
                     /score="22.8"
                     /tool="antismash"
                     /translation="VILDIIKEQLEIRSDDDSFGVNDSILSIGATSMDLVAIIHRINKC
                     LQPSQPLRLTDILKDSTARGLA"
     aSDomain        complement(1528..2817)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_08513_AMP-binding.1"
                     /evalue="1.70E-59"
                     /label="ANIA_08513_AMP-binding.1"
                     /locus_tag="ANIA_08513"
                     /protein_end="462"
                     /protein_start="32"
                     /score="193.3"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="DEGIIVYHPNSISTSSPPQTVSYKDLLHQAEANATRLLQQKLCSP
                     KSIVLVHFESALDSIVWYWSVLLAGGIPALTGPGMFSQNPADRERHLRHLSETLNSPVC
                     LTRPALLAPFEEQTADDRIKARTVDEILAAPEIADVADAPLPALTPSSTDMLALMLTSG
                     SSGNAKAVPLTHQQLLAAFRGKSTAASLRFPRSPFLSWVHMDHVANLVHCHIFAIVSGI
                     SQIQVPAPDLLINPAQLLNLISRHRVSRTFMPNFLCAKLRRQLESGSPEYILDPGLNLE
                     TLYIDTGGEANVTEVCIALQSLLSRYGAPDNVFKPSFGMTETVAGCIFNSHCPSYDHAQ
                     RHEFACLGKPMPGVRMRVTRLDTPSEEAAPGERGSLEVTGEVVFKGYYNNPAATAEAFT
                     SDGWFRTGDLAFIDSNGNLHLDGRTKEMINI"
     CDS_motif       complement(1624..1710)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_08513_0002"
                     /evalue="8.70E-06"
                     /label="NRPS-A_a6"
                     /locus_tag="ANIA_08513"
                     /protein_end="430"
                     /protein_start="401"
                     /score="18.2"
                     /tool="antismash"
                     /translation="PGERGSLEVTGEVVFKGYYNNPAATAEAF"
     CDS_motif       complement(2293..2346)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_ANIA_08513_0001"
                     /evalue="2.50E-03"
                     /label="NRPS-A_a3"
                     /locus_tag="ANIA_08513"
                     /protein_end="207"
                     /protein_start="189"
                     /score="11.0"
                     /tool="antismash"
                     /translation="LMLTSGSSGNAKAVPLTH"
     gene            3303..4695
                     /locus_tag="ANIA_08514"
                     /old_locus_tag="AN8514.4"
     mRNA            join(3303..3925,3990..4481,4551..4695)
                     /locus_tag="ANIA_08514"
                     /note="transcript_id=CADANIAT00003006"
                     /old_locus_tag="AN8514.4"
     CDS             join(3303..3925,3990..4481,4551..4695)
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00003006"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00003006"
                     /db_xref="GOA:Q5AT66"
                     /db_xref="InterPro:IPR017795"
                     /db_xref="UniProtKB/TrEMBL:Q5AT66"
                     /gene_functions="biosynthetic (rule-based-clusters) indole:
                     dmat"
                     /gene_kind="biosynthetic"
                     /locus_tag="ANIA_08514"
                     /note="transcript_id=CADANIAT00003006"
                     /old_locus_tag="AN8514.4"
                     /product="Putative uncharacterized proteinTdiB;
                     [Source:UniProtKB/TrEMBL;Acc:Q5AT66]"
                     /protein_id="CBF80712.1"
                     /sec_met_domain="dmat (E-value: 1.2e-115, bitscore: 375.2,
                     seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MATEYWSRHLRSVLAPLFAAAGTYSPEDQESHLAFIDEHIAPNLG
                     PLPWEPHGPYSTPSSLVGSPFDPSINIVSSGKAKVRFDFDVISPPDRTGPDPFAEGSAR
                     EILHRLADLVGADTQWMGYLMDALYLTPAEAEVAKTKLPPGVAIPPSSVGFDFDGPERT
                     LKFYIPSVRKALATGQDVSELMLKTLRGLQPLGSELVPAMDLIASYLSTRTNDAMLPLV
                     GIDCLDPRTHKNARVKCYLHTSSNSFAVVRDVLTLGGRLSDDTSLKRVETLKSVWPLLI
                     NELEGPQSDAATMDESWSKPERLNRTGYSGIQYTIEITPGQAIPDTKIYVPLFQYTDSS
                     EVAERNFESALKKLGNEWGLSGKYRSVMQEIFKDVENYGQTYASFSYTEGKGVYTTSYV
                     AMPIKDEGGGSLAGDFGFRN"
     gene            5346..6413
                     /locus_tag="ANIA_08518"
                     /old_locus_tag="AN8518.4"
     mRNA            5346..6413
                     /locus_tag="ANIA_08518"
                     /note="transcript_id=CADANIAT00003007"
                     /old_locus_tag="AN8518.4"
     CDS             5346..6413
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00003007"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00003007"
                     /db_xref="GOA:Q5AT62"
                     /db_xref="InterPro:IPR002085"
                     /db_xref="InterPro:IPR011032"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="UniProtKB/TrEMBL:Q5AT62"
                     /locus_tag="ANIA_08518"
                     /note="transcript_id=CADANIAT00003007"
                     /old_locus_tag="AN8518.4"
                     /product="Putative uncharacterized proteinTdiC;
                     [Source:UniProtKB/TrEMBL;Acc:Q5AT62]"
                     /protein_id="CBF80714.1"
                     /transl_table=11
                     /translation="MHAALVPTWSSPCPIYTEIPDPGPPPPEQLQLKVLAVGIPRVVRL
                     RARGIHPTAKSASLPYDPSIDGVGIDEQTGIMYYILPLSASCLAEKVNVDRDNLVPLQP
                     GAPKPQPRNGPENGYGIALGDAADHRAETLDPIAIAGLVNPVSSSWMALRTRVDGEITG
                     KTVLVLGATSKSGRAAVLVARFLGANKVIGVARREEGLRSVEGLDGWVTSGDMLPGETG
                     VRFALPDWVGPVHIVLDYVGGSVAAGVLGSAEIEEGRELQYVQVGNLALELGTGEKHMF
                     ETLPGHLISRKPICIRGSGMGSFSRRDLVREMPGLVAFLARMKAPFGIASAPMCEVASV
                     WQDEDTKGSRVVIVP"
     CDS             join(6630..6714,6766..6988,7023..7184,7302..7502,
                     7570..7818,7889..8303)
                     /NRPS_PKS="Domain: Aminotran_1_2 (119-433). E-value:
                     3.1e-26. Score: 84.2. Matches aSDomain:
                     nrpspksdomains_ANIA_08516_Aminotran_1_2.1"
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00003008"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00003008"
                     /db_xref="GOA:Q5AT64"
                     /db_xref="InterPro:IPR004839"
                     /db_xref="InterPro:IPR015421"
                     /db_xref="InterPro:IPR015422"
                     /db_xref="InterPro:IPR015424"
                     /db_xref="UniProtKB/TrEMBL:Q5AT64"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019:aminotransferase (Score: 223; E-value: 1.1e-67)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ANIA_08516"
                     /note="transcript_id=CADANIAT00003008"
                     /old_locus_tag="AN8516.4"
                     /product="L-tryptophan:phenylpyruvate aminotransferase
                     (Eurofung)"
                     /protein_id="CBF80716.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 2.6e-27, bitscore:
                     84.2, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGSIGANNAVADPTPLFSSRVQKWEPGAIRSLLPLEALPGMISLV
                     AGKPSPETFPIAEIAISLKDTPAGTGRIVVDGDELNQALQYGLPRGNAQLIQVCSVPYG
                     SKVFRGASTASMRTEAGRAALAHGSQELIHRVIQVFTDPGDPVLLETWVSLPLGVAGFL
                     RADGQELIPVYSDAQGLNPASLEQALSEWPGDSPRPKVLYTTPTGSNPTGQSCTESRKA
                     EILRLAKRFNFIILEDDAYYYLNYGDDKQRARSYLALERDVNGESGRVVRFDSLSKIVS
                     PGMRLGILTAQAAVVDKVVRITENINLQPSSTTQLLALSLLRHWGQAGFLKHCAEAAEV
                     YRRRRDVFVSAAERHLQGRATWVVPTAGMFVWLELKLPPEMDSFELLKSQGMKNGVLAI
                     PGVAFMPGNEQTCYIRVSFSLVPERDMDEACRRIAGLVDRCACHS"
     gene            6630..8348
                     /locus_tag="ANIA_08516"
                     /old_locus_tag="AN8516.4"
     mRNA            join(6630..6714,6766..6988,7023..7184,7302..7502,
                     7570..7818,7889..8348)
                     /locus_tag="ANIA_08516"
                     /note="transcript_id=CADANIAT00003008"
                     /old_locus_tag="AN8516.4"
     aSDomain        join(7072..7184,7302..7502,7570..7818,7889..8267)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ANIA_08516_Aminotran_1_2.1"
                     /evalue="3.10E-26"
                     /label="ANIA_08516_Aminotran_1_2.1"
                     /locus_tag="ANIA_08516"
                     /protein_end="433"
                     /protein_start="119"
                     /score="84.2"
                     /tool="antismash"
                     /translation="AGRAALAHGSQELIHRVIQVFTDPGDPVLLETWVSLPLGVAGFLR
                     ADGQELIPVYSDAQGLNPASLEQALSEWPGDSPRPKVLYTTPTGSNPTGQSCTESRKAE
                     ILRLAKRFNFIILEDDAYYYLNYGDDKQRARSYLALERDVNGESGRVVRFDSLSKIVSP
                     GMRLGILTAQAAVVDKVVRITENINLQPSSTTQLLALSLLRHWGQAGFLKHCAEAAEVY
                     RRRRDVFVSAAERHLQGRATWVVPTAGMFVWLELKLPPEMDSFELLKSQGMKNGVLAIP
                     GVAFMPGNEQTCYIRVSFSLVPERDMDEACRRI"
     gene            complement(8375..9759)
                     /locus_tag="ANIA_08520"
                     /old_locus_tag="AN8520.4"
     mRNA            complement(join(8375..8438,8503..9102,9224..9299,
                     9433..9445,9514..9527,9583..9680,9734..9759))
                     /locus_tag="ANIA_08520"
                     /note="transcript_id=CADANIAT00003009"
                     /old_locus_tag="AN8520.4"
     CDS             complement(join(8375..8438,8503..9102,9224..9299,
                     9433..9445,9514..9527,9583..9680,9734..9759))
                     /codon_start=1
                     /db_xref="EnsemblGenomes-Gn:CADANIAG00003009"
                     /db_xref="EnsemblGenomes-Tr:CADANIAT00003009"
                     /db_xref="GOA:Q5AT60"
                     /db_xref="InterPro:IPR029063"
                     /db_xref="UniProtKB/TrEMBL:Q5AT60"
                     /locus_tag="ANIA_08520"
                     /note="transcript_id=CADANIAT00003009"
                     /old_locus_tag="AN8520.4"
                     /product="conserved hypothetical protein"
                     /protein_id="CBF80718.1"
                     /transl_table=11
                     /translation="MVNRPYANMNDLANAVAESVQKYLDNSGQARNGLFQKTNNGERDI
                     ETERLVISPTVTSAICARYSNRRLNMGPCFWSQCYSRRKRRQRTMDIHPPVRLCPLPPA
                     SPAPRRAPTLQAGVQASTIRLIVLSPVSLAKSSLTPGGWLEMQELSNPVTSDDGTLSEN
                     NPLSQWGRLLIEASKKMNRPVDNPAKYETWMREAGFVNCHTVAYNWPTNPWPADEKGKT
                     LGLWNLYNVLQRFEEFSVALLVKVLGWEMDDAKTFLGNVKEELMNEGVHGYWPVYVVYG
                     QKPAAPGSDVITDSE"
ORIGIN
        1 ctacaggccc cgctccctca gtacattctt caatgtccct gcaaaagcct caacatactc
       61 ccgattcaac atggtgtaat gtgctccttg cacatcgtgg aaacgcacgt cctcccgcac
      121 aaactccttc caggcactta acctcccctc cacccagtcg attcggtcct ttgcaacatg
      181 gctcaaaggg tccgccacaa acacatccat acacttgact gatcccgagg gttcatagtc
      241 caccgccaga ctctgcatat tagatgctag acctacccag agaagatagt actcctctga
      301 aaggccgagc tcgtcccacc gtgcggcatc gcagtgctgg cgcaggtacc ggatggcgtc
      361 cagacggcgg tttgcccgat taaactcctg cagagtaggt ttatgcgtat acgctgccag
      421 ctcggtcatc agaccgacga agtagaatag atggatcacg cactcctccc agacgagctc
      481 acgcatgcgg aacttgatat gcggcggaag attccagctc ccgcagtagc ggacctcatc
      541 gccgtcctgt tcgagaagct tgctgacttc aaatgccacc atcccgccaa acgagtaccc
      601 cgctattgcg tagggcccgt ggggctggcg ttctttaatt gcgtcgcggt aggttgtgaa
      661 caattcctcc agtgaggtga agggggtttc gggcaagccg gccgccgcgt tgaagccttt
      721 tgcgcggaag gcgtaaacgg gtcggtctgt aatgtgatgg gccaggttca cgaagactag
      781 gacctcaccg acgcccgggt gtaccagcca tagaggactc ttggtcccgt gcggttgcag
      841 ggtcacgacg gggtcgtaga cgtgcgtgga agactgatcc tgtgagcgag gcgcggcccc
      901 tgttgcgagg gcgacggcta gccccctggc tgtcgagtct ttgaggatgt ctgttaggcg
      961 gaggggctga gaaggctgta ggcacttatt gatgcggtgg atgatagcga ctagatccat
     1021 cgacgtggcg cctattgaga ggattgaatc gttgacgcca aagctgtcat catcagaccg
     1081 gatctccagc tgttccttga taatatccag aattactgct tcatccggcg tctctgggct
     1141 cgcacgggtc ttttgctggt agcgtcttat agcctcgtcg ttgatctgct gctgcgtagc
     1201 gaactggcct tcttccagag ccgtcttcag ttttgcgcgc gacagttttc ccagcgtgct
     1261 ctttggcata tcctgcggac gcagcggcac tacgcgcggc cgggaccgcg tgtgcatggc
     1321 cacgacacgg atgatgctgc tttgcgtgct gaacctggct tcgtcatcgc tctccacata
     1381 agatggaagg tagagcacaa ccacgacctc ggtatccatg gttgcatcgc ggctgctgaa
     1441 cgtgcagaag taactaggtg ttgcgcctgg gatctgcgcc tgctcgagag cagcatccag
     1501 ttcgtacggg aggtatttga ctccattgat gttgatcatc tccttcgtgc gcccgtcgag
     1561 gtgcagattg ccgttgctgt caatgaacgc cagatccccc gtccggaacc atccatcgct
     1621 ggtgaacgcc tctgctgtgg cggcaggatt attgtagtaa cctttaaaga caacttcccc
     1681 ggttacttcg aggctgccgc gctcaccggg ggctgcctct tcgctcggag tgtcaagccg
     1741 tgtcacccgc attcgcactc caggcatcgg tttcccgaga caggcgaact catggcgctg
     1801 ggcgtgatca tagcttgggc agtgcgagtt gaagatacat ccggccacgg tttcggtcat
     1861 accgaaggag ggcttgaaaa cgttgtcggg agccccgtac cggctgagga gggattggag
     1921 tgcaatacaa acctctgtga cgttcgcctc accaccggta tcaatataga gcgtctcaag
     1981 gttgaggccg gggtccagga tatactctgg actccccgac tccagctgtc gccgcaactt
     2041 ggcgcagagg aagttcggca tgaacgtgcg cgagacgcgg tgtctgctta tcaggttaag
     2101 aagctgagcc gggttgatga gaagatccgg agcagggact tgaatctgtg atatgccgga
     2161 cacgatggcg aagatatggc agtggactag attggcgacg tggtccatgt gcacccagga
     2221 gaggaacggg ctgcggggga agcggaggct ggccgcggtg gacttgcccc tgaaggccgc
     2281 aaggagctgt tgatgggtca gagggacagc ttttgcgttg ccgctgctcc cggaggtcag
     2341 catgagggca agcatatcgg tcgaagacgg ggttagggca ggcagaggtg cgtcagcaac
     2401 gtccgcaatt tcgggagctg cgaggatctc atccactgtt cgagctttga tccggtcatc
     2461 cgctgtctgc tcttcaaagg gggccaagag ggcaggccgg gtcagacaga ccggtgaatt
     2521 gagcgtctcg gacagatgac gcagatgcct ctctctatct gccgggttct ggctgaacat
     2581 cccaggcccg gtgagggcag gtatgccccc agccagaagg acagaccagt accatacgat
     2641 gctgtccagt gcggactcaa agtgaacgag gacaatggac ttggggctac atagcttctg
     2701 ctgcaacagt ctggtggcat tcgcctctgc ctgatgcagc agatctttgt aggagactgt
     2761 ctgtggaggt gatgaggtgc tgatgctgtt tgggtggtat actataatgc cctcatcggt
     2821 atgagcagca gcatgtcgaa gagcgtccac gatgttgcca aacgggtact tggctgccct
     2881 gagcggtgcg atctcggtct tgcttggtgc catcttgtta cagtctaaga ggaggtccta
     2941 gcctggccag aagggtctca atgagtgagt tatgagtaag ttgggtgagc cactgtgcct
     3001 gtttctccgc actcaagaca ctttaagtat gcagcctgcc ctaatacgag atattcccgt
     3061 cctcgcgggg taagtccaaa tcaggcccgt gttgcacaat gacgatacta ttattattac
     3121 tcgttcatat tacacgctga cgggatacga ggttgcattc cgccacacga gataccaatt
     3181 caaggtcaca aaaggacaag ctgcagccgg gcctggacca tggggcgtat atatgatgac
     3241 agtagagtac tctgaaattc cttgcaaaca gtcctttctt ttgcgcagaa actgtcttca
     3301 tcatggctac agaatactgg tcccgtcatc tacgctcagt gctggctccg ctgttcgctg
     3361 cagctggcac atactctcct gaagatcagg agtcccatct ggccttcatt gacgagcaca
     3421 ttgcgcccaa cctgggccct ctcccttggg agccccatgg accctacagc actccttcct
     3481 ccctcgtggg ctcccccttc gaccccagca tcaacatcgt ctcatccgga aaggccaagg
     3541 tccgtttcga ctttgacgtg atcagtccac ctgatcgaac aggcccagac ccctttgcag
     3601 agggatccgc cagggagatc ctccaccgtc tcgccgacct tgtcggcgca gacacacagt
     3661 ggatgggcta cctcatggat gctctctacc tgacccccgc ggaggctgag gttgcgaaaa
     3721 cgaagttgcc tccaggtgtt gctatcccgc ccagctcagt gggcttcgac ttcgacggcc
     3781 ccgagcggac gctgaagttc tacatcccca gtgtgcggaa agcgctagca acggggcagg
     3841 atgtgtccga actcatgcta aagactctac gtggattaca gccacttggg tctgagctag
     3901 tgccagcgat ggacctgatt gcttcgtaag ctctaacttc acatctctag attattctgt
     3961 attagcgcga tgcaagctga ctgaaccagg tatctctcaa cccgcaccaa cgacgccatg
     4021 ctcccgctcg tcgggattga ctgcctcgat ccaaggacac ataagaacgc tcgggtcaaa
     4081 tgctacctgc acacgagcag caacagcttc gcagtcgtcc gcgacgtcct cacgctggga
     4141 ggccgtctca gcgacgacac ctcgctcaag cgagtcgaga cactcaaatc ggtctggcct
     4201 ttgctgatca acgagctaga aggtccacag agcgacgcgg ccaccatgga cgaatcctgg
     4261 tccaagccag agcggctcaa ccggacaggg tactcgggga tccagtacac gattgagatt
     4321 acccccggtc aggcaatccc cgacacgaag atctacgtcc cgttattcca gtacacggac
     4381 agctctgagg tcgctgagcg gaactttgag agcgcgctaa agaagctggg gaatgagtgg
     4441 gggctgagtg gcaagtatag gagcgtcatg caggaaatct tgtgagtcat ttcttctatt
     4501 tcatttatct attttttatt gttatacgat cccttctgac ggccgcaaag caaggatgta
     4561 gagaactatg gccagacgta cgcatctttc tcctacacgg agggaaaggg cgtctatacg
     4621 acctcgtatg ttgctatgcc tattaaggat gagggagggg gtagcctcgc tggagatttc
     4681 ggtttcagga actagatcaa gtactcggtg ctgtagcgct caatcagata ctagtctaat
     4741 caaatcattg gctgtcgttg attcggagtg taggaactat ttcttagtag acatcttcct
     4801 ctcactaccc aagacctccg ccaaaaccta gcacccaaat gagcgcggag catccggctg
     4861 tcccccacat tgacatcaga tgccaagcat cgagcaaagt aaagaaaggt ctcgaccaga
     4921 tctctgcctc attccttttg agtgggattt cgtcagctgc atgcccagaa agctagggct
     4981 gcttagtacg ggacgggcca gctgcacctg cagcttggct tcgtatccca gaatagccaa
     5041 catttatccg gagactcgca cattgtatcc ccttgtggcg attcgagatt ttgagcttct
     5101 ttctatcagg gtctaattct tactgcataa tgcggggttg attgtcgtac gtactaaggt
     5161 caacatgcac acggcctacc tgcaaccttt cagcctacaa gatgtaaccc aagaaaaccc
     5221 agagtataaa aaagcgcaaa taatataggc tgacctcgac tccatctcat tcttcagcct
     5281 accatcttgc tatttaggtt aactccgcat cgcagagaaa cgactcaccc agaacggaaa
     5341 cgactatgca tgccgctctc gtcccaacct ggtcctcccc ctgtcccatc tataccgaga
     5401 ttccagaccc ggggcctccg ccaccagaac agctccaact gaaagtcttg gccgttggga
     5461 ttccccgcgt tgtacggctc cgtgctcgag ggatccaccc gactgccaag tcagccagtc
     5521 tcccgtacga ccccagcatt gacggcgtag ggattgacga acaaaccggc attatgtact
     5581 acattcttcc gctctcggcg tcttgtcttg cggagaaagt gaatgtggac cgggacaatc
     5641 tggtcccgct ccagcccggg gcacctaaac ctcaacctcg aaatggacct gaaaatggat
     5701 atggaattgc acttggcgat gctgctgacc atagagctga gacgctggac cccatcgcaa
     5761 ttgcggggct cgtgaatccc gtgtcgagta gctggatggc gttgagaaca agggtggatg
     5821 gcgaaattac ggggaaaacg gtcctggttc tgggggcaac gagtaagagt gggcgagccg
     5881 cagtgctcgt tgcaaggttt ctgggagcaa ataaagtgat tggagtggcg aggagagagg
     5941 aagggctaag aagtgtggaa gggttggatg gctgggttac ctcgggggat atgcttcctg
     6001 gggagaccgg ggttaggttt gctctaccgg actgggttgg gccagtgcat atcgtcctgg
     6061 attacgttgg aggcagcgtt gccgcgggag tcctgggcag tgctgagatt gaagagggta
     6121 gagagttgca atacgtgcag gttgggaacc tcgcgcttga gctgggcacg ggagagaagc
     6181 atatgtttga aaccttacca ggccacctga tcagccggaa gcccatctgc atccgcggat
     6241 cagggatggg aagtttcagt aggagggatc tggtccgaga gatgcctggc ttggtggcat
     6301 ttcttgcacg aatgaaggcg ccgtttggga ttgcgagcgc gccgatgtgc gaggtggcgt
     6361 cggtttggca ggatgaggat acgaagggaa gccgagtggt gattgtacct tgaacgacac
     6421 ttgtacgaac tggggcggtt tgtcatgaca cattcaacac ggactgacag ttacaatcat
     6481 ggtagtagta gcctgagatc ccgaatacga agccattcct gtggttcatc gccttctatg
     6541 ctatatagag gcttttaatt ttgctggtgt ctagtatcaa acccactact gtagaccatc
     6601 gtgctactga atatcgtacc aacatcacca tgggctcaat aggggccaat aatgctgtgg
     6661 cggacccaac tcctcttttc tcgtcacggg tgcagaaatg ggagcccggt gcaagtaagc
     6721 cacgttggcc atgacagcgc aattccaagc tgaccagatc ttcagtccgg agcctactcc
     6781 ctctcgaagc ccttcctggc atgatctccc tcgtggcagg aaaacccagt cccgagacgt
     6841 tccccattgc tgagatcgcc atctcactca aggatactcc agccgggaca ggtaggatcg
     6901 tggttgatgg ggacgagctc aaccaggctc ttcaatatgg tcttcctcga ggaaatgccc
     6961 agctaatcca ggtttgctct gtcccttagt ctccatattt cctactgatc attgttgttc
     7021 agtggttcga aagtcttcag aggagcgtcc acggcctcga tgagaacgga ggctggtcgt
     7081 gctgcattgg cacatggaag tcaggagctc attcatcgcg tcatccaggt cttcactgat
     7141 cctggggacc cagttcttct cgaaacgtgg gtctcactcc ctttgtatgc attcccggac
     7201 gcaggcgccg ctgactgaag ccatagacca gcatatccgt atgatttcct ccaggaattt
     7261 tcagagtgga cattttagca ccttgctgac agggaccaca gaggcgtcgc cgggttcctt
     7321 cgcgcagacg gacaggagct tatcccggtc tactctgacg cccagggcct caatccggcc
     7381 agcctggagc aggcattgtc ggagtggccc ggagactcgc cgcgccccaa agtcctctat
     7441 acaaccccga ctgggtcgaa cccaaccggt caatcctgca ccgagagccg caaggccgag
     7501 atgtaattac tcctctgccc agccactgct ccagacctga gactgacagt tccatcccga
     7561 cacctatagt ctccgtctgg caaagcgatt caattttatc atcctcgaag acgacgcata
     7621 ctattatctc aactacggag acgacaaaca gagagcgcgg agctatctcg ccctcgaaag
     7681 agacgtcaat ggagagtcag gccgcgtcgt gcgtttcgac tcgctcagca agatcgtcag
     7741 tcccggcatg cgtctgggga tcctcacagc acaagcagcg gttgttgaca aggtggtgcg
     7801 gatcactgaa aatataaagt atgtttatcc ttctctcttc tcctgtcctg ttctggctgc
     7861 ttcaaatgac tcgctcactg aatcccagcc ttcaaccctc ctccacaact caacttctag
     7921 ccctctctct actccgccac tggggtcaag ctggcttcct gaaacactgt gcagaagccg
     7981 ccgaggtcta tcgacgtcgc cgagacgtct ttgtttctgc ggcggagcgg catctccagg
     8041 gtcgagctac atgggtggtt ccaacggccg gcatgtttgt ctggctggaa cttaagttgc
     8101 caccggaaat ggatagcttc gagcttctga agagccaggg aatgaagaat ggggttcttg
     8161 ctattcctgg tgttgccttc atgcctggga acgagcaaac gtgctatatc cgggtgagct
     8221 tcagtctagt tcctgagaga gatatggatg aggcgtgtag gcggatcgcg ggtctagttg
     8281 atcgatgtgc ttgccattcc taagcttatt atacttttgt tcgatggttc taatactatt
     8341 ttcccggtca aaagatcttc gccatgaatt tggttcactc gctatctgta atcacgtcgg
     8401 atccaggtgc agctggcttc tgcccataca caacatatct attcgctcga ctcagcatat
     8461 aagacctcgt aggacagtca tgaaaaggga tggtagactt acaccggcca gtaaccatgc
     8521 acaccctcat tcatcagctc ctcctttacg ttccccaaga acgtctttgc atcatccatc
     8581 tcccatccaa gtacttttac caacaaagcc accgaaaact cttcaaagcg ctgcaacaca
     8641 ttgtacagat tccaaagacc caaggtctta cccttttcat ccgcaggcca cgggttcgtg
     8701 ggccaattat acgcaacggt gtggcaattc acgaatccgg cctcgcgcat ccacgtctca
     8761 tatttggcag gattgtccac gggacggttc atcttctttg aggcctctat aagaagacgg
     8821 ccccattggg agagcgggtt gttctctgat agggttccgt cgtcgcttgt gacggggtta
     8881 ctgagttctt gcatctccag ccagccgccg ggagtgaggg agctttttgc cagtgagacc
     8941 gggctcagca cgattaagcg aatggtactt gcctgaacgc ctgcttgaag agtcggggct
     9001 cgtctaggcg ccggtgatgc tggcggcaat ggacaaagtc gaactggcgg gtgtatgtcc
     9061 atggttcgtt ggcgtcgctt gcgtctggaa taacactgag acctagaggt attttacttg
     9121 tgggactagg atcgttggcc acgatatacg cagaaggatt ctgttcagct gctaccggtt
     9181 aggggatatc tagtgtatat agaccgagaa tgctgcagcc taccaaaagc atggacccat
     9241 gttgagtctc ctgttgctat atcgagcaca gattgcactg gtgacggtag gggagataac
     9301 tgaagtttcc catctagggt tagatggaag agctggtgct ggagtgcttg gtggtgtcag
     9361 tctggtacgc cttgaagttt ctatgcaacc actctatctt atccacgtag acggacgatg
     9421 gactaagctt accaagtcgc tctgtctgtg agacaggttt cgcgtcagca ttgcgttcat
     9481 agtcagggat cataaacact gcctgatacg gacctcgata tcccgttctg taaggggctc
     9541 aattagctta gactggtaac gtatacatct aaatcttcat acctccatta ttggttttct
     9601 gaaacagtcc attcctcgct tgtccactgt tgtccaggta cttttgtacg gactcggcga
     9661 ccgcattggc aaggtcgttc ctgggtcagt tagatattgc tgtaaaatat gtctactagt
     9721 gtcaaggacg tacatgtttg cgtacgggcg gttaaccat
//
