BGC0000443 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000443 (Pseudomonas sp. SHC52)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS,NRPS-like	1	40,038	thanamycin	NRPS:Type I	100%

BGC0000443 - Region 1 - NRPS,NRPS-like

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Location: 1 - 40,038 nt. (total: 40,038 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 40,039
Label		BGC0000443

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000443	1.56	NRP	thanamycin	Pseudomonas sp. SHC52
BGC0002071	0.85	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.74	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135	0.71	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001133	0.70	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002518	0.70	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001128	0.67	NRP	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132	0.67	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286	0.65	NRP	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758	0.64	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000443	1.00	NRP	thanamycin	Pseudomonas sp. SHC52
BGC0002071	0.77	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.70	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135	0.70	NRP	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002518	0.68	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001758	0.68	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001128	0.68	NRP	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001131	0.67	NRP	ambactin	Xenorhabdus miraniensis
BGC0001629	0.67	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0001132	0.67	NRP	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 40038:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - Orn - X - X - His) + (Thr - Thr - X) + (Thr)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 2, location 15639 - 40038:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - Orn - X - X - His) + (Thr - Thr - X) + (Thr)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AED90002.1

Search NORINE for peptide: strict or relaxed

AMP-binding (477..880): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHLSLIDK
Nearest Stachelhaus code(s):: Ser DVWHLSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1541..1943): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DMENMGAVNK
Nearest Stachelhaus code(s):: Orn DMENMGAVNK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2627..3029): X

nrpys: OH-Asp, Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DMKDLGMVDK
Nearest Stachelhaus code(s):: OH-Asp or Asp DMKDLGMVDK (100% 8Å match); Asp DMKDLGMVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3685..4084): X

nrpys: Hse

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DLKNVGSDVK
Nearest Stachelhaus code(s):: Hse DLKNVGSDVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4757..5161): His

nrpys: His

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DSALIAEVWK
Nearest Stachelhaus code(s):: His DSALIAEVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AED90003.1

Search NORINE for peptide: strict or relaxed

AMP-binding (961..1361): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2021..2429): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3114..3516): X

nrpys: OH-Asp, Asp

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asp (Asp)

Stachelhaus prediction details:

Stachelhaus sequence:: DMKDLGMVDK
Nearest Stachelhaus code(s):: OH-Asp or Asp DMKDLGMVDK (100% 8Å match); Asp DMKDLGMVDK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AED90004.1

Search NORINE for peptide: strict or relaxed

AMP-binding (26..427): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWSVGMVHK
Nearest Stachelhaus code(s):: Thr DFWSVGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)