BGC0000447: tolaasin I biosynthetic gene cluster from Pseudomonas costantinii
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Location: 1 - 69,124 nt. (total: 69,124 nt).
This entry is originally from NCBI GenBank HE967327.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000447
Short description tolaasin I biosynthetic gene cluster from Pseudomonas costantinii
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Biosynthetic class(es)
  • NRPS (Type I)
Loci
HE967327.1
via Knock-out studies
Compounds
  • tolaasin I
  • tolaasin F
Species Pseudomonas costantinii [taxonomy]
References
Chemical products information
tolaasin I Evidence:
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C94H163N21O25
Chemical database entries
PubCHEM
ChemSpider
tolaasin F Evidence:
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C94H163N21O25
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • CCJ67633.1
  • mepC
 1 - 1440 (-) multidrug/solvent efflux pump outer membrane protein mepC
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    • CCJ67634.1
    		 1503 - 2384 (-) bacterial regulatory, luxR family protein
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      • CCJ67635.1
      		 2636 - 4333 (+) cyclic peptide transporter family protein
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        • CCJ67636.1
        • taaA
        		 4795 - 14454 (+) TaaA
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          • CCJ67637.1
          • taaB
          		 14451 - 24293 (+) TaaB
          • Scaffold biosynthesis
          • Knock-out
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          • CCJ67638.1
          • taaC
          		 24290 - 34258 (+) TaaC
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            • CCJ67639.1
            • taaD
            		 34255 - 44247 (+) TaaD
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              • CCJ67640.1
              • taaE
              		 44558 - 65716 (+) TaaE
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                • CCJ67641.1
                • macA
                		 65838 - 66989 (+) macrolide-specific efflux protein macA
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                  • CCJ67642.1
                  • macB
                  		 66992 - 68950 (+) macrolide export ATP-binding/permease protein MacB
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                    Biosynthesis information

                    Biosynthetic modules

                    Name
                    1
                    Type
                    nrps-type1
                    Genes
                    taaA
                    Substrates
                    threonine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (Starter), adenylation
                    Name
                    2
                    Type
                    nrps-type1
                    Genes
                    taaA
                    Substrates
                    proline (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    3
                    Type
                    nrps-type1
                    Genes
                    taaA
                    Substrates
                    serine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    4
                    Type
                    nrps-type1
                    Genes
                    taaB
                    Substrates
                    leucine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    5
                    Type
                    nrps-type1
                    Genes
                    taaB
                    Substrates
                    valine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    6
                    Type
                    nrps-type1
                    Genes
                    taaB
                    Substrates
                    serine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    7
                    Type
                    nrps-type1
                    Genes
                    taaC
                    Substrates
                    leucine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    8
                    Type
                    nrps-type1
                    Genes
                    taaC
                    Substrates
                    valine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    9
                    Type
                    nrps-type1
                    Genes
                    taaC
                    Substrates
                    valine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    10
                    Type
                    nrps-type1
                    Genes
                    taaD
                    Substrates
                    glutamine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    11
                    Type
                    nrps-type1
                    Genes
                    taaD
                    Substrates
                    leucine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    12
                    Type
                    nrps-type1
                    Genes
                    taaD
                    Substrates
                    valine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation, adenylation
                    Name
                    13
                    Type
                    nrps-type1
                    Genes
                    taaE
                    Substrates
                    threonine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    14
                    Type
                    nrps-type1
                    Genes
                    taaE
                    Substrates
                    threonine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    15
                    Type
                    nrps-type1
                    Genes
                    taaE
                    Substrates
                    leucine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Name
                    16
                    Type
                    nrps-type1
                    Genes
                    taaE
                    Substrates
                    homoserine (evidence: Structure-based inference)
                    Integrated Monomers
                    Domains
                    condensation (LCL), adenylation
                    Name
                    17
                    Type
                    nrps-type1
                    Genes
                    taaE
                    Substrates
                    2,4-diaminobutyric acid (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (LCL), adenylation
                    Name
                    18
                    Type
                    nrps-type1
                    Genes
                    taaE
                    Substrates
                    lysine (evidence: Sequence-based prediction)
                    Integrated Monomers
                    Domains
                    condensation (DCL), adenylation
                    Annotation changelog

                    Entry version: 4

                    Date
                    Changes
                    Submitters
                    Reviewers
                    2022-10-07
                    Update chemical activity to schema version 2.11
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                    Entry version: 3

                    Date
                    Changes
                    Submitters
                    Reviewers
                    2022-09-15
                    Corrected NRP module activity
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                    2022-09-15
                    Updated bioactivity data
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                    2022-09-15
                    Updated NRP substrate specificities
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                    Entry version: 2

                    Date
                    Changes
                    Submitters
                    Reviewers
                    2019-10-16
                    Migrated from v1.4
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                    Entry version: 1

                    Date
                    Changes
                    Submitters
                    Reviewers
                    2015-06-12
                    Submitted
                    • (ID: F65XGYSSLMUEJC2Q4T3BLCPS)
                    • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                    Detailed domain annotation
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