BGC0000447

General information about the BGC

MIBiG accession	BGC0000447
Short description	tolaasin I biosynthetic gene cluster from Pseudomonas costantinii
Status	Minimal annotation: no A minimal annotation only contains information on the BGC loci and one or more linked chemical product(s) Completeness: complete Whether the loci encodes everything needed for the pathway producing the compound(s)
Biosynthetic class(es)	NRP (Other lipopeptide)
Loci	NCBI GenBank: HE967327.1
Compounds	tolaasin I tolaasin F
Species	Pseudomonas costantinii [taxonomy]
References	Biosynthesis and mass spectrometric imaging of tolaasin, the virulence factor of brown blotch mushroom disease. Scherlach K et al., Chembiochem (2013) PMID:24222604 In silico study of the ion channel formed by tolaasin I produced by Pseudomonas tolaasii. Jo G et al., J Microbiol Biotechnol (2011) PMID:22031037 Purification of a pore-forming peptide toxin, tolaasin, produced by Pseudomonas tolaasii 6264. Cho KH et al., J Biochem Mol Biol (2007) PMID:17244491 Tolaasins A--E, five new lipodepsipeptides produced by Pseudomonas tolaasii. Bassarello C et al., J Nat Prod (2004) PMID:15165142

Chemical products information

tolaasin I

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C₉₄H₁₆₃N₂₁O₂₅

Chemical database entries
PubCHEM
ChemSpider

tolaasin F

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C₉₄H₁₆₃N₂₁O₂₅

List of genes involved in compound(s) production

Identifiers	Position	Product	Functions	Evidence	Extra
CCJ67633.1 mepC	1 - 1440 (-)	multidrug/solvent efflux pump outer membrane protein mepC			copy AA seq copy Nt seq
CCJ67634.1	1503 - 2384 (-)	bacterial regulatory, luxR family protein			copy AA seq copy Nt seq
CCJ67635.1	2636 - 4333 (+)	cyclic peptide transporter family protein			copy AA seq copy Nt seq
CCJ67636.1 taaA	4795 - 14454 (+)	TaaA			copy AA seq copy Nt seq
CCJ67637.1 taaB	14451 - 24293 (+)	TaaB	Scaffold biosynthesis	Knock-out	copy AA seq copy Nt seq
CCJ67638.1 taaC	24290 - 34258 (+)	TaaC			copy AA seq copy Nt seq
CCJ67639.1 taaD	34255 - 44247 (+)	TaaD			copy AA seq copy Nt seq
CCJ67640.1 taaE	44558 - 65716 (+)	TaaE			copy AA seq copy Nt seq
CCJ67641.1 macA	65838 - 66989 (+)	macrolide-specific efflux protein macA			copy AA seq copy Nt seq
CCJ67642.1 macB	66992 - 68950 (+)	macrolide export ATP-binding/permease protein MacB			copy AA seq copy Nt seq

NRP-specific information

Subclass	Other lipopeptide
Cyclic?	yes
Release type	Macrolactonization
Lipid moiety	beta-hydroxy-octanoic acid
Thioesterase genes	taaE (Type I)

NRP-synthases

Gene	Modules
taaA	Module 1 Specificity: threonine Evidence for specificity: Sequence-based prediction Condensation domain type: Starter Modification domains: dehydration
	Module 2 Specificity: proline Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
	Module 3 Specificity: serine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
taaB	Module 4 Specificity: leucine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
	Module 5 Specificity: valine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
	Module 6 Specificity: serine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
taaC	Module 7 Specificity: leucine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
	Module 8 Specificity: valine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
	Module 9 Specificity: valine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
taaD	Module 10 Specificity: glutamine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
	Module 11 Specificity: leucine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
	Module 12 Specificity: valine Evidence for specificity: Sequence-based prediction Condensation domain type: Unknown
taaE	Module 13 Specificity: threonine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL Modification domains: dehydration
	Module 14 Specificity: threonine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
	Module 15 Specificity: leucine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL
	Module 16 Specificity: homoserine Evidence for specificity: Structure-based inference Condensation domain type: LCL
	Module 17 Specificity: 2,4-diaminobutyric acid Evidence for specificity: Sequence-based prediction Condensation domain type: LCL
	Module 18 Specificity: lysine Evidence for specificity: Sequence-based prediction Condensation domain type: DCL

Annotation changelog

MIBiG version	Submitter	Notes
1.0	Hidden contributor (ID: F65XGYSSLMUEJC2Q4T3BLCPS, no GDPR consent given).	Submitted
2.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Migrated from v1.4
3.0	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Corrected NRP module activity Updated bioactivity data Updated NRP substrate specificities
3.1	Hidden contributor (ID: AAAAAAAAAAAAAAAAAAAAAAAA, no GDPR consent given).	Update chemical activity to schema version 2.11

Detailed domain annotation

Selected features only

Show module domains

Similar known gene clusters

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