BGC0000452 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000452 (Brevibacillus brevis NBRC 100599)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	49,087	tyrocidine	NRPS:Type I	100%

BGC0000452 - Region 1 - NRPS

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Location: 1 - 49,087 nt. (total: 49,087 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 49,088
Label		BGC0000452

Detailed domain annotation

Selected features only

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Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000452	1.00	NRP	tyrocidine	Brevibacillus brevis NBRC 100599
BGC0000426	0.49	NRP	sevadicin	Paenibacillus larvae
BGC0000400	0.41	NRP	paenibacterin	Paenibacillus sp. OSY-SE
BGC0001843	0.38	NRP	tridecaptin M	Paenibacillus sp. M-152
BGC0002437	0.36	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001133	0.36	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.35	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.34	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.34	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001844	0.34	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000452	1.00	NRP	tyrocidine	Brevibacillus brevis NBRC 100599
BGC0000426	0.78	NRP	sevadicin	Paenibacillus larvae
BGC0000400	0.73	NRP	paenibacterin	Paenibacillus sp. OSY-SE
BGC0001843	0.70	NRP	tridecaptin M	Paenibacillus sp. M-152
BGC0002437	0.69	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0001133	0.68	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.68	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002135	0.68	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002518	0.67	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001844	0.67	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 49087:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (D-Phe) + (Pro - Phe - D-Phe) + (Asn - Gln - Tyr - Val - Orn - Leu)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

BBR47_27870

Search NORINE for peptide: strict or relaxed

AMP-binding (37..430): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTIAAICK
Nearest Stachelhaus code(s):: Phe DAWTIAAICK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BBR47_27880

Search NORINE for peptide: strict or relaxed

AMP-binding (472..870): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQSIAHVVK
Nearest Stachelhaus code(s):: Pro DVQSIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1512..1906): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALVMGAVIK
Nearest Stachelhaus code(s):: Phe DALVMGAVIK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2550..2940): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTIAAICK
Nearest Stachelhaus code(s):: Phe DAWTIAAICK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

BBR47_27890

Search NORINE for peptide: strict or relaxed

AMP-binding (470..863): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DLTKIGEVGK
Nearest Stachelhaus code(s):: Asn DLTKIGEVGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1505..1900): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: gln (Gln)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWQFGLIDK
Nearest Stachelhaus code(s):: Gln DAWQFGLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2542..2938): Tyr

nrpys: Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Polar aromatic ring (Tyr, bOH-Tyr)
Single AA prediction:: tyr (Tyr)

Stachelhaus prediction details:

Stachelhaus sequence:: DGLTTGEVVK
Nearest Stachelhaus code(s):: Tyr DGLTTGEVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3582..3975): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAFWIGGTFK
Nearest Stachelhaus code(s):: Val DAFWIGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4617..5019): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Long positively charged side chain (Orn, Lys, Arg)
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DVGEIGSIDK
Nearest Stachelhaus code(s):: Orn DVGEIGSIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5663..6060): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: DGAYTGEVVK
Nearest Stachelhaus code(s):: Leu DGAYTGEVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)