BGC0000459: griseoviridin biosynthetic gene cluster from Streptomyces griseoviridis
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Location: 13,530 - 118,200 nt. (total: 104,671 nt).
This entry is originally from NCBI GenBank JX508597.1.

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core biosynthetic genes
additional biosynthetic genes
transport-related genes
regulatory genes
other genes
resistance
TTA codons
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Gene details
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General
Compounds
Genes
Biosynthesis
History
NRPS/PKS domains
KnownClusterBlast
General information about the BGC
MIBiG accession BGC0000459
Short description griseoviridin biosynthetic gene cluster from Streptomyces griseoviridis
Status Quality: questionable
The quality level of this entry.

Status: active
The status of this entry.

Completeness: complete
Whether the entry covers everything needed for the pathway producing the compound(s)
Remarks "VG is synthesised by the NRPS within this cluster whereas GV is hybrid PK-NRP and as such utilises the PKS machinery also."
Biosynthetic class(es)
  • NRPS (Type I)
  • PKS (Trans-AT type I)
Loci
JX508597.1
13530 - 118200
via Knock-out studies
Compounds
  • griseoviridin
  • fijimycin A
Species Streptomyces griseoviridis [taxonomy]
References
Chemical products information
griseoviridin [synonyms: pristinamycin IIA, virginiamycin m] Evidence: NMR [1]
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C22H27N3O7S1
Chemical database entries
NPAtlas
fijimycin A Evidence: MS/MS [2]
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C44H62N8O11
Chemical database entries
NPAtlas
List of genes involved in compound(s) production
Identifiers Position Product Functions Evidence Extra
  • AGN74872.1
 13530 - 14696 (+) SARP-type regulator
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    • AGN74873.1
    		 14816 - 16408 (-) major facilitator transporter
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      • AGN74874.1
      		 16726 - 17847 (-) branched-chain amino acid aminotransferase
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        • AGN74875.1
        		 17916 - 18935 (-) methyltransferase
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          • AGN74876.1
          		 19073 - 26989 (-) nonribosomal peptide synthetase
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            • AGN74877.1
            		 27311 - 28057 (+) type ii thioestrerase
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              • AGN74878.1
              		 28052 - 29134 (-) flavin-dependent oxidoreductase
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                • AGN74879.1
                		 29368 - 29613 (-) hypothetical protein
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                  • AGN74880.1
                  		 29782 - 31404 (+) 3-hydroxypicolinic acid:AMP ligase
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                    • AGN74881.1
                    		 31408 - 32709 (+) L-lysine 2-aminotransferase
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                      • AGN74882.1
                      		 32763 - 32951 (+) 4-oxalocrotonate tautomerase
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                        • AGN74883.1
                        		 33036 - 34343 (-) phenylacetyl-CoA dioxygenase
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                          • AGN74884.1
                          		 34358 - 34609 (-) MbtH domain-containing protein
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                            • AGN74885.1
                            		 34683 - 51854 (-) nonribosomal peptide synthetase
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                              • AGN74886.1
                              		 51851 - 56554 (-) nonribosomal peptide synthetase
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                                • AGN74887.1
                                		 57108 - 58265 (+) branched-chain alpha-keto acid dehydrogenase E1 subunit alpha
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                                  • AGN74888.1
                                  		 58262 - 59317 (+) branched-chain alpha-keto acid dehydrogenase E1 subunit beta
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                                    • AGN74889.1
                                    		 59323 - 60720 (+) branched-chain alpha-keto acid dehydrogenase E2
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                                      • AGN74890.1
                                      		 60959 - 62614 (+) ABC transporter
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                                        • AGN74891.1
                                        		 62773 - 63963 (-) P450-like monooxygenase
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                                          • AGN74892.1
                                          		 64158 - 77825 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
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                                            • AGN74893.1
                                            		 77828 - 84568 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
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                                              • AGN74894.1
                                              		 84740 - 91246 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
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                                                • AGN74895.1
                                                		 91257 - 99092 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
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                                                  • AGN74896.1
                                                  		 99089 - 104953 (+) nonribosomal peptide synthetase/polyketide synthase hybrid protein
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                                                    • AGN74897.1
                                                    		 104950 - 105900 (+) acyltransferase
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                                                      • AGN74898.1
                                                      		 105894 - 108320 (+) nonribosomal peptide synthetase
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                                                        • AGN74899.1
                                                        		 108570 - 109964 (+) EmrB/QacA subfamily drug resistance transporter
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                                                          • AGN74900.1
                                                          		 109996 - 110889 (-) streptogramin B lyase
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                                                            • AGN74901.1
                                                            		 111137 - 112285 (-) sarcosine oxidase
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                                                              • AGN74902.1
                                                              		 112395 - 113237 (-) SARP-type regulator
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                                                                • AGN74903.1
                                                                		 113627 - 114274 (-) TetR-type regulator
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                                                                  • AGN74904.1
                                                                  		 114756 - 115520 (+) ketoreductase
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                                                                    • AGN74905.1
                                                                    		 115710 - 116678 (+) AfsA-like gamma-butyrolactone biosynthetic protein
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                                                                      • AGN74906.1
                                                                      		 116703 - 117386 (-) gamma-butyrolactone receptor
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                                                                        • AGN74907.1
                                                                        		 117511 - 118200 (-) phosphatase
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                                                                          Biosynthesis information

                                                                          Biosynthetic modules

                                                                          Name
                                                                          0
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74880.1
                                                                          Substrates
                                                                          3-hydroxypicolinic acid (evidence: Sequence-based prediction)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          adenylation
                                                                          Name
                                                                          1
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74876.1
                                                                          Substrates
                                                                          threonine (evidence: Structure-based inference)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          condensation (LCL), adenylation
                                                                          Name
                                                                          2
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74876.1
                                                                          Substrates
                                                                          leucine (evidence: Structure-based inference)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          condensation (LCL), adenylation, epimerase
                                                                          Name
                                                                          A3
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74886.1
                                                                          Substrates
                                                                          4R-hydroxyproline (evidence: Structure-based inference)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          condensation (DCL), adenylation, epimerase
                                                                          Name
                                                                          4
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74885.1
                                                                          Substrates
                                                                          glycine (evidence: Structure-based inference)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          condensation (DCL), adenylation, methyltransferase
                                                                          Name
                                                                          5
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74885.1
                                                                          Substrates
                                                                          3-methylleucine (evidence: Structure-based inference)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          condensation (LCL), adenylation, methyltransferase
                                                                          Name
                                                                          6
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74885.1
                                                                          Substrates
                                                                          alanine (evidence: Structure-based inference)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          condensation (LCL), adenylation
                                                                          Name
                                                                          A7
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74885.1
                                                                          Substrates
                                                                          phenylglycine (evidence: Structure-based inference)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          condensation (LCL), adenylation, methyltransferase
                                                                          Name
                                                                          B3
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74892.1
                                                                          Substrates
                                                                          glycine (evidence: Structure-based inference)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          condensation, adenylation
                                                                          Name
                                                                          B7
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74895.1
                                                                          Substrates
                                                                          serine (evidence: Structure-based inference)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          adenylation
                                                                          Name
                                                                          B9
                                                                          Type
                                                                          nrps-type1
                                                                          Genes
                                                                          AGN74898.1
                                                                          Substrates
                                                                          cysteine (evidence: Structure-based inference)
                                                                          Integrated Monomers
                                                                          Domains
                                                                          adenylation
                                                                          Name
                                                                          B1
                                                                          Type
                                                                          pks-trans-at
                                                                          Genes
                                                                          AGN74892.1
                                                                          Substrates
                                                                          Integrated Monomers
                                                                          Domains
                                                                          ketosynthase, ketoreductase, carrier (ACP)
                                                                          Name
                                                                          B2
                                                                          Type
                                                                          pks-trans-at
                                                                          Genes
                                                                          AGN74892.1
                                                                          Substrates
                                                                          Integrated Monomers
                                                                          Domains
                                                                          ketosynthase, dehydratase, ketoreductase, carrier (ACP)
                                                                          Name
                                                                          B4
                                                                          Type
                                                                          pks-trans-at
                                                                          Genes
                                                                          AGN74893.1
                                                                          Substrates
                                                                          Integrated Monomers
                                                                          Domains
                                                                          ketosynthase, dehydratase, ketoreductase, carrier (ACP)
                                                                          Name
                                                                          B5
                                                                          Type
                                                                          pks-trans-at
                                                                          Genes
                                                                          AGN74894.1
                                                                          Substrates
                                                                          Integrated Monomers
                                                                          Domains
                                                                          ketosynthase, ketoreductase, carrier (ACP)
                                                                          Name
                                                                          B6
                                                                          Type
                                                                          pks-trans-at
                                                                          Genes
                                                                          AGN74894.1
                                                                          Substrates
                                                                          Integrated Monomers
                                                                          Domains
                                                                          ketosynthase, carrier (ACP)
                                                                          Name
                                                                          B8
                                                                          Type
                                                                          pks-trans-at
                                                                          Genes
                                                                          AGN74895.1
                                                                          Substrates
                                                                          Integrated Monomers
                                                                          Domains
                                                                          ketosynthase, carrier (ACP)
                                                                          Name
                                                                          0
                                                                          Type
                                                                          pks-trans-at-starter
                                                                          Genes
                                                                          AGN74892.1
                                                                          Substrates
                                                                          Integrated Monomers
                                                                          Domains
                                                                          carrier (ACP)
                                                                          Annotation changelog

                                                                          Entry version: next

                                                                          Date
                                                                          Changes
                                                                          Submitters
                                                                          Reviewers
                                                                          2024-05-14
                                                                          MIBiG v4 annotathon
                                                                          • (ID: P75FRHHCFPTCPYSP65VDVPHW)
                                                                          • (ID: 4ZBJCWV5NZUBDJXCSIV4TKIA)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                          Entry version: 4

                                                                          Date
                                                                          Changes
                                                                          Submitters
                                                                          Reviewers
                                                                          2022-10-07
                                                                          Update chemical activity to schema version 2.11
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                          Entry version: 3

                                                                          Date
                                                                          Changes
                                                                          Submitters
                                                                          Reviewers
                                                                          2022-09-15
                                                                          Corrected NRP module activity
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          2022-09-15
                                                                          Removed ketoreductase stereochemistry annotation from modules without ketoreductases
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          2022-09-15
                                                                          Sorted modules by module number
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          2022-09-15
                                                                          Corrected gene identifiers
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          2022-09-15
                                                                          Updated bioactivity data
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          2022-09-15
                                                                          Updated NRP substrate specificities
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                          Entry version: 2

                                                                          Date
                                                                          Changes
                                                                          Submitters
                                                                          Reviewers
                                                                          2019-10-16
                                                                          Migrated from v1.4
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          2019-10-16
                                                                          Updated compound(s) information (NPAtlas curation)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)

                                                                          Entry version: 1

                                                                          Date
                                                                          Changes
                                                                          Submitters
                                                                          Reviewers
                                                                          2015-06-12
                                                                          Submitted
                                                                          • (ID: AADADFAEMFE2AOMR24EUYNFH)
                                                                          • (ID: AAAAAAAAAAAAAAAAAAAAAAAA)
                                                                          Detailed domain annotation
                                                                          Shows NRPS- and PKS-related domains for each feature that contains them. Click on each domain for more information about the domain's location, consensus monomer prediction, and other details.
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