BGC0000461 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000461 (Lysobacter enzymogenes)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	63,720	WAP-8294A2	NRPS:Type I	100%

BGC0000461 - Region 1 - NRPS

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Location: 1 - 63,720 nt. (total: 63,720 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 63,721
Label		BGC0000461

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000461	1.00	NRP	WAP-8294A2	Lysobacter enzymogenes
BGC0001133	0.43	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.40	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002437	0.33	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0002518	0.33	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001844	0.32	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001833	0.31	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001131	0.31	NRP	ambactin	Xenorhabdus miraniensis
BGC0001843	0.31	NRP	tridecaptin M	Paenibacillus sp. M-152
BGC0001758	0.30	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000461	1.00	NRP	WAP-8294A2	Lysobacter enzymogenes
BGC0001133	0.74	NRP	taxlllaid A	Xenorhabdus bovienii SS-2004
BGC0002071	0.72	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0002437	0.66	NRP	thermoactinoamide A	Thermoactinomyces sp. AS95
BGC0002518	0.66	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001844	0.65	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0001833	0.65	NRP	icosalide A, icosalide B	Burkholderia gladioli
BGC0001131	0.64	NRP	ambactin	Xenorhabdus miraniensis
BGC0001843	0.64	NRP	tridecaptin M	Paenibacillus sp. M-152
BGC0001758	0.64	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known gene clusters from MIBiG 4.0

Module view

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NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 63720:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ser - D-X - Ser - Gly - D-NMe-Phe - Leu - D-Orn) + (Glu - D-Asn - D-Trp - D-Orn - NMe-Val)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

AEP18655.1

Search NORINE for peptide: strict or relaxed

AMP-binding (465..867): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHVGSIGK
Nearest Stachelhaus code(s):: Glu DVWHVGSIGK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1531..1944): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DGTKLGEVGK
Nearest Stachelhaus code(s):: Asn DGTKLGEVGK (100% 8Å match); OH-Asn or Asn DGTKLGEVGK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3062..3469): Trp

nrpys: Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAGLVGEVWK
Nearest Stachelhaus code(s):: Trp DAGLVGEVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4602..5010): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: Orn and hydroxy-Orn specific (Orn, OH-Orn)
Single AA prediction:: orn (Orn)

Stachelhaus prediction details:

Stachelhaus sequence:: DTEDMGLVDK
Nearest Stachelhaus code(s):: Orn DTEDMGLVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6134..6536): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAYWWGGTFK
Nearest Stachelhaus code(s):: Val DAYWWGGTFK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AEP18656.1

Search NORINE for peptide: strict or relaxed

AMP-binding (459..862): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHVSLIDK
Nearest Stachelhaus code(s):: Ser DVWHVSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1514..1918): X

nrpys: OH-Asn, Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DGTKLGEVGK
Nearest Stachelhaus code(s):: OH-Asn or Asn DGTKLGEVGK (100% 8Å match); Asn DGTKLGEVGK (91% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3063..3466): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: DVWHVSLIDK
Nearest Stachelhaus code(s):: Ser DVWHVSLIDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4126..4524): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: DILQLGVVWK
Nearest Stachelhaus code(s):: Gly DILQLGVVWK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5190..5583): Phe

nrpys: Phe

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: phe (Phe)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWTIAAVCK
Nearest Stachelhaus code(s):: Phe DAWTIAAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (7136..7542): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAMLIGAICK
Nearest Stachelhaus code(s):: Leu DAMLIGAICK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (8199..8619): Orn

nrpys: Orn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DTEDMGYVDK
Nearest Stachelhaus code(s):: Orn DTEDMGYVDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)