Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000462.1:
Cluster 1NRP112151
The following clusters are from record BGC0000462.2:
Cluster 2NRP131000

BGC0000462, WLIP biosynthetic gene cluster from Pseudomonas putida. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: WLIP
SMILES string: Copy to clipboard
Molecular formula: C54H95N9O16
Exact molecular mass: 1126.3828 Da (None)
Molecular activity: Antibacterial, Other

Class-specific details

Biosynthetic class(es):
NRP
Nonribosomal peptide subclass:
Other lipopeptide (cyclic)
3-hydroxydecanoic acid
Thioesterase type:
Type II
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
wlpA (AFJ23819.1)
Module 1
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Starter
Module 2
A specificity: Glutamate
Evidence for specificity: Structure-based inference
C domain subtype: Dual
wlpB (AFJ23825.1)
Module 3
A specificity: Allo-threonine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 4
A specificity: Valine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 5
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 6
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
wlpC (AFJ23826.1)
Module 7
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 8
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 9
A specificity: Isoleucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual

Gene cluster description

WLIP (BGC0000462). Gene Cluster 1. Biosynthetic class = NRP. GenBank JN982332, positions 1116-10056. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Rene De Mot (University of Leuven (KU Leuven))

Literature references

1. Rokni-Zadeh H et al. (2012) Genetic and functional characterization of cyclic lipopeptide white-line-inducing principle (WLIP) production by rice rhizosphere isolate Pseudomonas putida RW10S2. Appl Environ Microbiol 78(14):4826-34. doi: 10.1128/AEM.00335-12. Epub

BGC0000462, WLIP biosynthetic gene cluster from Pseudomonas putida. Locus 2. Full MIBiG entry.

Chemical compounds

Compound: WLIP
SMILES string: Copy to clipboard
Molecular formula: C54H95N9O16
Exact molecular mass: 1126.3828 Da (None)
Molecular activity: Antibacterial, Other

Class-specific details

Biosynthetic class(es):
NRP
Nonribosomal peptide subclass:
Other lipopeptide (cyclic)
3-hydroxydecanoic acid
Thioesterase type:
Type II
Release / cyclization type:
Macrolactonization

Nonribosomal peptide synthetases:
wlpA (AFJ23819.1)
Module 1
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Starter
Module 2
A specificity: Glutamate
Evidence for specificity: Structure-based inference
C domain subtype: Dual
wlpB (AFJ23825.1)
Module 3
A specificity: Allo-threonine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 4
A specificity: Valine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 5
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 6
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
wlpC (AFJ23826.1)
Module 7
A specificity: Leucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual
Module 8
A specificity: Serine
Evidence for specificity: Structure-based inference
C domain subtype: LCL
Module 9
A specificity: Isoleucine
Evidence for specificity: Structure-based inference
C domain subtype: Dual

Gene cluster description

WLIP (BGC0000462). Gene Cluster 2. Biosynthetic class = NRP. GenBank JN982333, positions 3354-30958. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Domain annotation

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Rene De Mot (University of Leuven (KU Leuven))

Literature references

1. Rokni-Zadeh H et al. (2012) Genetic and functional characterization of cyclic lipopeptide white-line-inducing principle (WLIP) production by rice rhizosphere isolate Pseudomonas putida RW10S2. Appl Environ Microbiol 78(14):4826-34. doi: 10.1128/AEM.00335-12. Epub