BGC0000464 - 1 region(s) - antiSMASH results

Identified secondary metabolite regions using strictness 'relaxed'

BGC0000464 (Xenorhabdus doucetiae)

Region	Type	From	To	Most similar known cluster		Similarity
Region 1	NRPS	1	105,987	xenoamicin A/xenoamicin B	NRPS:Type I	100%

BGC0000464 - Region 1 - NRPS

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Location: 1 - 105,987 nt. (total: 105,987 nt) Show pHMM detection rules used

Region on contig edge.

NRPS: cds(Condensation and (AMP-binding or A-OX))

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core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

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Gene details

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Gene overview

MIBiG

NRPS/PKS domains

MIBiG comparison

KnownClusterBlast

NRPS/PKS modules

Gene/CDS overview

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Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

External annotation

Name		MIBiG
Version		4.0alpha

Type		Subregion
Location		1 - 105,988
Label		BGC0000464

Detailed domain annotation

Selected features only

Show module domains

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000464	1.00	NRP	xenoamicin A, xenoamicin B	Xenorhabdus doucetiae
BGC0002071	0.48	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001629	0.43	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0002135	0.41	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758	0.39	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001131	0.38	NRP	ambactin	Xenorhabdus miraniensis
BGC0001641	0.37	NRP	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001844	0.37	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002518	0.37	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001806	0.36	NRP	tolaasin A	Pseudomonas tolaasii

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000464	1.00	NRP	xenoamicin A, xenoamicin B	Xenorhabdus doucetiae
BGC0002071	0.77	NRP	virginiafactin A, virginiafactin B, virginiafactin C, virginiafactin D	Pseudomonas sp. QS1027
BGC0001629	0.74	NRP	jessenipeptin	Pseudomonas sp. QS1027
BGC0002135	0.72	NRP	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758	0.71	NRP	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001131	0.70	NRP	ambactin	Xenorhabdus miraniensis
BGC0001641	0.70	NRP	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001844	0.70	NRP	holrhizin	Paraburkholderia rhizoxinica HKI 454
BGC0002518	0.69	NRP	syringafactin A, syringafactin C	Pseudomonas sp. SZ57
BGC0001806	0.69	NRP	tolaasin A	Pseudomonas tolaasii

Similar known gene clusters from MIBiG 4.0

Module view

Legend

NRPS/PKS products

NRPS/PKS substrates

Predicted core structure(s)

For candidate cluster 1, location 0 - 105987:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Pro - Ala - Val - Leu - Ile - Thr - Leu) + (Leu - Leu) + (Ala - bAla - Pro - Leu) + (Leu - X - X - Gln - bAla - Asn - Val) + (Val - Ile) + (Ile - Ile - Gln - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

XDD1_2281

Search NORINE for peptide: strict or relaxed

AMP-binding (512..928): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1588..1985): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DIFNNALIYK
Nearest Stachelhaus code(s):: Ala DIFNNALIYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2673..3076): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWWLGGTFK
Nearest Stachelhaus code(s):: Val DAWWLGGTFK (100% 8Å match); Leu DAWWLGGTFK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3742..4149): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWLLGAVCK
Nearest Stachelhaus code(s):: Leu DAWLLGAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (4846..5253): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGMTFK
Nearest Stachelhaus code(s):: Ile DAWFLGMTFK (100% 8Å match); Leu DAWFLGMTFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5917..6332): Thr

nrpys: Thr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: thr (Thr)
Single AA prediction:: thr (Thr)

Stachelhaus prediction details:

Stachelhaus sequence:: DFWNIGMVHK
Nearest Stachelhaus code(s):: Thr DFWNIGMVHK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (7012..7415): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWWLGGTFK
Nearest Stachelhaus code(s):: Leu DAWWLGGTFK (100% 8Å match); Val DAWWLGGTFK (97% 8Å match)
Stachelhaus code match:: 100% (strong)

XDD1_2282

Search NORINE for peptide: strict or relaxed

AMP-binding (521..933): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGMTFK
Nearest Stachelhaus code(s):: Leu DAWFLGMTFK (100% 8Å match); Ile DAWFLGMTFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1630..2033): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWWLGGTFK
Nearest Stachelhaus code(s):: Leu DAWWLGGTFK (100% 8Å match); Val DAWWLGGTFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

XDD1_2283

Search NORINE for peptide: strict or relaxed

AMP-binding (510..938): Ala

nrpys: Ala

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Tiny, hydrophilic, transition to aliphatic (Gly, Ala)
Single AA prediction:: ala (Ala)

Stachelhaus prediction details:

Stachelhaus sequence:: DLYNNALTYK
Nearest Stachelhaus code(s):: Ala DLYNNALTYK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1647..2066): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VDTVVSFGDK
Nearest Stachelhaus code(s):: bAla VDTVVSFGDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (2727..3143): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: DVQFIAHVVK
Nearest Stachelhaus code(s):: Pro DVQFIAHVVK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (3807..4210): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWWLGGTFK
Nearest Stachelhaus code(s):: Leu DAWWLGGTFK (100% 8Å match); Val DAWWLGGTFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

XDD1_2286

Search NORINE for peptide: strict or relaxed

AMP-binding (219..621): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWLLGAVCK
Nearest Stachelhaus code(s):: Leu DAWLLGAVCK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1284..1677): X

nrpys: Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DALILAGIWK
Nearest Stachelhaus code(s):: Phe DAWVLAGIQK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (2366..2764): X

nrpys: Phe, Trp

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: Unpolar aromatic ring (Phe, Trp)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DGFALVGVCK
Nearest Stachelhaus code(s):: Leu DIFALGGVAK (50% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (3428..3828): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASDLGVVDK
Nearest Stachelhaus code(s):: Gln DAQDLGVVDK (76% 8Å match); Ile DAQDLGVVDK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (4512..4927): bAla

nrpys: bAla

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: VDTVVSFGDK
Nearest Stachelhaus code(s):: bAla VDTVVSFGDK (100% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (5587..5994): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: asn (Asn)

Stachelhaus prediction details:

Stachelhaus sequence:: DATKIGEVGK
Nearest Stachelhaus code(s):: Asn DATKIGEVGK (71% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (6682..7089): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWWLGGTFK
Nearest Stachelhaus code(s):: Val DAWWLGGTFK (97% 8Å match); Leu DAWWLGGTFK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

XDD1_2287

Search NORINE for peptide: strict or relaxed

AMP-binding (510..921): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: DAWWLGGTFK
Nearest Stachelhaus code(s):: Val DAWWLGGTFK (91% 8Å match); Leu DAWWLGGTFK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1614..2021): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGVTFK
Nearest Stachelhaus code(s):: Ile DAWFLGMTFK (97% 8Å match)
Stachelhaus code match:: 90% (moderate)

XDD1_2288

Search NORINE for peptide: strict or relaxed

AMP-binding (510..921): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGVTFK
Nearest Stachelhaus code(s):: Ile DAWFLGMTFK (97% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (1608..2019): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DAWFLGVTFK
Nearest Stachelhaus code(s):: Ile DAWFLGMTFK (97% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (2708..3109): Gln

nrpys: Gln

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DASDLGVVDK
Nearest Stachelhaus code(s):: Gln DAQDLGVVDK (76% 8Å match); Ile DAQDLGVVDK (76% 8Å match)
Stachelhaus code match:: 90% (moderate)

AMP-binding (3798..4224): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: DTSDIGSVIK
Nearest Stachelhaus code(s):: Lys DTEDIGSVIK (71% 8Å match)
Stachelhaus code match:: 90% (moderate)