Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000468.1:
Cluster 1RiPP117580

BGC0000468, bottromycin A2 biosynthetic gene cluster from Streptomyces bottropensis. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: Bottromycin A2
ChemSpider ID: 26365309
SMILES string: Copy to clipboard
Molecular formula:
Average molecular mass: Da
Molecular activity: Antibacterial
Molecular target: Bacterial ribosome

Class-specific details

Biosynthetic class(es):
RiPP
RiPP subclass:
Bottromycin (cyclic)
Peptides in this cluster:
BMBC/CCM09443.1
RiPP core peptide sequence(s): GPVVVFDC
Leader peptide length: 1
Follower peptide length: 35
Peptidase(s) involved in precursor cleavage: bmbk, ccm09452.1, bmbg, ccm09447.1, bmbh, ccm09448.1
Crosslinks in final product:
1=4 AA (Lactam)

Gene cluster description

bottromycin A2 (BGC0000468). Gene Cluster 1. Biosynthetic class = RiPP. GenBank HE983629, positions 1377-18956. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Homologous known gene clusters

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Knock-out studies, Heterologous expression
MIxS-compliance:Unknown
Contact for this cluster:Juan Pablo Gomez-Escribano (John Innes Centre; Norwich Research Park; Norwich; NR4 7UH; UK)

Literature references

1. Shimamura H et al. (2009 TI) Structure determination and total synthesis of bottromycin A2: a potent antibiotic against MRSA and VRE. Angew Chem Int Ed Engl 48(5):914-7. doi: 10.1002/anie.200804138.
2. Otaka T, Kaji A. (1983) Mode of action of bottromycin A2: effect of bottromycin A2 on polysomes. FEBS Lett 153(1):53-9.
3. Otaka T, Kaji A. (1981) Mode of action of bottromycin A2: effect on peptide bond formation. FEBS Lett 123(2):173-6.
4. Juan Pablo Gomez-Escribano, Lijiang Song, Mervyn J. Bibb, Gregory L. Challis. (2012) Posttranslational b-methylation and macrolactamidination in the biosynthesis of the bottromycin complex of ribosomal peptide antibiotics. Chemical Science, vol. 3, no. 12, p. 3522