LOCUS       BGC0000470             14745 bp    DNA     linear   BCT 17-OCT-2012
DEFINITION  Streptomyces sp. WMMB 272 bottromycin D gene cluster, partial
            sequence.
ACCESSION   BGC0000470
VERSION     BGC0000470
KEYWORDS    .
SOURCE      Streptomyces sp. WMMB 272
  ORGANISM  Streptomyces sp. WMMB 272
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:42:19
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14745
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..1260)
                     /codon_start=1
                     /gene="bstK"
                     /gene_functions="transport (smcogs) SMCOG1020:major
                     facilitator transporter (Score: 194; E-value: 6.8e-59)"
                     /gene_kind="transport"
                     /product="MFS-family/H+ antiporter"
                     /protein_id="AFU90407.1"
                     /transl_table=11
                     /translation="MLTRYLLSHGLSLLGNTVATIALPWLVYLRTGDAAAVGLVAAASA
                     LPMLVSAVAGGVLIDRVGRRRLSVGADFASAACVAALPIIDHFSGLTVVWFVVLGVAGA
                     LFDVPGMTARETLAPDLAAASGVGIERLAGLRESVGGVVVVVGPAVAGGLIVLVDPATV
                     LWITSGCSAAAALVTLSLPRTVGARSASAPASEGGGLKADIARGFSVLRHDRVVTAVTF
                     VSAGSAAILAPLQNLVLPVYLVGKDSPGGFGLVISFLAVGGIVGSGLYAAIGARLSRRA
                     VFVVAQLLSTAGIVGLALLPPIPILLGAAVVAGIGAGPLPALCLVLVTDRIPESVRGRV
                     LGLQNAVSMTAIPLGTLGASLLIEWGGLRQTGALLGLGWAVVAVSVLALPALRQLDKVR
                     QQEPEEAVGNEPTDPAVAGQ"
     protocluster    1..14745
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[9449:10292]"
                     /cutoff="20000"
                     /detection_rule="botH"
                     /neighbourhood="10000"
                     /product="bottromycin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      9450..10292
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="botH"
                     /neighbourhood="10000"
                     /product="bottromycin"
                     /protocluster_number="1"
     cand_cluster    1..14745
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="botH"
                     /kind="single"
                     /product="bottromycin"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14745
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000470"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14745
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="bottromycin"
                     /region_number="1"
                     /rules="botH"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..1260)
                     /gene="bstK"
     gene            complement(1521..2351)
                     /gene="bstB"
     CDS             complement(1521..2351)
                     /codon_start=1
                     /gene="bstB"
                     /product="O-methyltransferase"
                     /protein_id="AFU90406.1"
                     /transl_table=11
                     /translation="MKVPLTGAAATLLAPLYARALDASSPHPMLADSTAPELVQKIEYD
                     FGALGMRPSNAVGVALRARFFDRRTREFLTAHPNSTVLHLGCGLDNRVDRVERGPGVRW
                     FDVDQPEVIEVREQLFSPRSDHRTIAASVTETDWLAEVPTDLPVLVIAEGLTMYLAADE
                     GPRMLRALVEHFSEGEAVFDTYARYAVRSTRSMALFRKTGARLAWGVDDPRELEREVPG
                     LRLVKSVKAYHTADPADFRRLPFALRTRMRVDAEVLARLPVLGRVGHLSRYAFG"
     gene            2876..4837
                     /gene="bstC"
     CDS             2876..4837
                     /codon_start=1
                     /gene="bstC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bottromycin_Methyltransferase_RRE"
                     /gene_functions="other (smcogs) SMCOG1277:Radical SAM
                     domain protein (Score: 169; E-value: 2.5e-51)"
                     /gene_kind="biosynthetic-additional"
                     /product="radical SAM-dependent protein"
                     /protein_id="AFU90405.1"
                     /sec_met_domain="TIGR03975 (E-value: 8e-161, bitscore:
                     525.8, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="Bottromycin_Methyltransferase_RRE
                     (E-value: 8.6e-43, bitscore: 134.0, seeds: 12, tool:
                     rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPALGGDDSVGNGRSATLVSMPWNSVVRPSIQVGTLRTVAEEEG
                     WQVTSEYAYLSFYGLGREMLGFSESEWADAYETISEKLYHMAVGDWIFARRHGESQQRD
                     RYFAELREMRVDDETIALIDSLRPVAERHVEETAAALLASDPDVIGFTSMFSQNGPTLA
                     VAERLRAEGYPGVIVLGGSNCEGSMGRALLANYPTVEAVVDGPGEDAFVALLRQVAAGG
                     PVRSQGRLLTRRPAEDSSDVGEAKISPTGLSVPVPNYDGFFEQLHAAGLEGLEAEITLP
                     VEFSRGCWWGAKTHCTFCGLNGATMAYRSKDPDSVADELQYLMDRYGVFDFFAVDNILD
                     LKYLDTALPLVEKLNTDHSLFFEVKANMEWADIRSMRRAGVRSVQPGIESLSTEGLRLL
                     KKGSTAFQNVRFLLGCAEYGVRADWNILTGYPKETPESIRAQLDIVASLSHLTPPHITL
                     IRFDRFSPYVESPESFGLSLSKPLPGYRYVYPELSGEDLWNIAYHFEGDFHDDVRNVDI
                     RRRLAARVRLWRNHHEQARFTYRLGFDSLTLSDHRPGLPAHETVLQGDDAALFRAVIGG
                     TRIRDLQANGWPEGQPWDRAWEKLQNWQRRRWIFIEGTKIIALAIREQPSAYRTPPPKG
                     TPRRQARNPVPVTLTSRK"
     gene            4922..5062
                     /gene="bstA"
     CDS             4922..5062
                     /codon_start=1
                     /gene="bstA"
                     /product="hypothetical protein"
                     /protein_id="AFU90404.1"
                     /transl_table=11
                     /translation="MGPAVVFDCMTADFLNDDPNNAELSSLEMEELESWGAWSDDTDQS
                     V"
     gene            5126..6301
                     /gene="bstD"
     CDS             5126..6301
                     /codon_start=1
                     /gene="bstD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) YcaO"
                     /gene_kind="biosynthetic-additional"
                     /product="YcaO protein"
                     /protein_id="AFU90403.1"
                     /sec_met_domain="YcaO (E-value: 6.5e-14, bitscore: 41.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADSSATDCVLREVAFRSYRAEHTVTVQCTVRPDSGPGQAEGYGT
                     ATTEAVARKKALSEAVERLLACTPFALIARRPTPRTDRSRGTDPWQRGLRSAPAGCQVR
                     NYRSLSGEQPRQVPLFWSSPWVAGEEMRSANLAPQAARLSSTVGWAVAPSPEAALQGAL
                     YELTELLNYGAFLYRCLAAPRERPAPGGGPEPREEGRIQTVPISWATRTPTILAVTRSS
                     DRLMPATGIGSGTTASEAAERAFLELAQAETLWRANSTAVPAERYFMRRFEKWPLLRRC
                     AALDFDLGEHAGPFDDGPHPSPLGALESRAIEVWADPGAVDISGADLRRTRLCFAHVVS
                     DPQPLLGLVRAGIPVFDTGEVRKILVCPRRARSADRSPSGRVAHSGRPTRA"
     gene            6219..7553
                     /gene="bstE"
     CDS             6219..7553
                     /codon_start=1
                     /gene="bstE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) YcaO"
                     /gene_kind="biosynthetic-additional"
                     /product="YcaO protein"
                     /protein_id="AFU90402.1"
                     /sec_met_domain="YcaO (E-value: 9.4e-36, bitscore: 113.6,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVHAEHDLLTAALLDGSLTQDVPPERETPIGRALSAASDWLSSE
                     QLTPDIWKYGLDSAPTYEVHLKDAAGTLCSRSSGKGLGHRSMASALFESAEHYHLDWRR
                     DPLADEAAFVPGREVAGQSTAAGSALLHRLGGILGDSPVLTREYRPVAEALGATGGAAP
                     PHRHPVFLRDGGYRNWPHPSDDGSFKPLWHYTTSAGYAAGTTLHEALVHAVNELIERDA
                     WSYQLARSYFGLPDESGRALRVIDHESLPAELRALVDDVEASSGSPILIVSIPCDTGVP
                     AYVVCDAESDEDVRLIGSGASPVQAYALQRGLLEYLQVCTMYQHGPVDAEREAAQIATA
                     LARYPRHLAAAGFDVRQLPHERQPFDSDGLPAAATPEELLRHLIDRLQGVGVDLHFRVL
                     TRPGTITVVDVVAPGLEMLDKARAGYPVLPTGRLGERLRPQRREP"
     gene            7550..9463
                     /gene="bstF"
     CDS             7550..9463
                     /codon_start=1
                     /gene="bstF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bottromycin_Methyltransferase_RRE"
                     /gene_functions="other (smcogs) SMCOG1277:Radical SAM
                     domain protein (Score: 122.3; E-value: 3.6e-37)"
                     /gene_kind="biosynthetic-additional"
                     /product="radical SAM-dependent protein"
                     /protein_id="AFU90401.1"
                     /sec_met_domain="TIGR03975 (E-value: 3.2e-137, bitscore:
                     447.9, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="Bottromycin_Methyltransferase_RRE
                     (E-value: 3.5e-36, bitscore: 112.9, seeds: 12, tool:
                     rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLVLVAPPWNSLYRPSIQIATLAALSTEEQGDPISAVYGYLDWF
                     EYAVGFLECDPEQFAAVYDTIGEELYGLGVGDWIFGGGLPGAGDADEASATAEYGAFLR
                     EQGVDEEIVADVLRLREAAPRFVADLAERLVDDGAECFGFTTSFSQLVPSLAIAVAIKE
                     RAPDRLVLLGGANCDRPMGQAVMHAYPFVDAVVEGEGEQAVNALTRAKSVDDLAAVPGI
                     VLRHEDGLRTALPTIQPVESAPVPKYDAYFERLESLPWGDLIAPHVALPFQMSRGCWWG
                     EKLQCTFCGENGSGMVYRSKPGSQLVDTLRTLTERHGAFNTYAVDTILAKNDNGLAALA
                     DAEVDITTFFEVKANLGPREVGELRAAGVTMVQPGIESLSSGALKLLRKGATSWHNLRF
                     LVLCQELGIDAQWNLLSAMPDESAEMLEEQLRLIPFITHLAPPSTVSKVRVDRYSPYFE
                     KPDEFGIELRGPEKKYRFIHRGEGVDRTALAYSFEHGQQAGKERDDDAAMLALRRRLGA
                     RVGLWKKLHAQSRFSFRFGPNVTVVHDYRPHVGTGRTVLRGADDLLFRSLVHGGRLMQA
                     VGRVGEQTGTPESRLHERVSEWAACGWVHLEGRRGLVLAVRDGMRDELGRAAALRGSDA
                     ES"
     gene            9450..10292
                     /gene="bstG"
     CDS             9450..10292
                     /codon_start=1
                     /gene="bstG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     bottromycin: botH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262:haloalkane dehalogenase (Score: 55.1; E-value:
                     1.1e-16)"
                     /gene_kind="biosynthetic"
                     /product="alpha-beta hydrolase"
                     /protein_id="AFU90400.1"
                     /sec_met_domain="botH (E-value: 7.8e-147, bitscore: 476.9,
                     seeds: 5, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 5e-14, bitscore: 42.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 2.4e-12, bitscore:
                     37.5, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRSPDREQTERFEVASVDGTLLRGFSRAARGSSDARGEPETLVLL
                     HGVAMDHRVWSESSFLDALPDDVHVVAPDLRGRGESDPGHSAEGHAIDRYVEDVRAVLG
                     FFGRERYSLFGLYFGGRIALRVAEADRRVQRAFSFCAHGEAVEVPADAVEEEAKEIEGP
                     GGYAYLRDHFSAAGAPAWMVSACDRVDQDELAAATRGLLHGSHQRAERGAADQDLVLIT
                     AEGDADLGTFREGSARLGARLWLVDSRTRVRAAARLAEVGTRTAELLTAAPAAMKGDA"
     gene            10410..11681
                     /gene="bstH"
     CDS             10410..11681
                     /codon_start=1
                     /gene="bstH"
                     /product="amidohydrolase"
                     /protein_id="AFU90399.1"
                     /transl_table=11
                     /translation="MLDAETGEAAPPRDLEVVDGAVARWSPAPDAAYCEWFALPGFVDA
                     HAHVSSVTDLVGMLAYGVTGYRQMWGEPAHLYSAGVHRARHAVLPLPWTTAGVVDGPDS
                     RIPHHVTVVDDARQVRRVVEDVLAFGFDGIKVYDDLARPAFEALVREAERAGIPVVGHV
                     PEAVPLDAAYPGMWSTEHLYGIVPNVFRLPAADRWAVLADALQRHRDDGPAVEGAAGRF
                     VCPTLTAWRARSGERRYTRPSRTLLQSATPSRRPAWHAAARDALSLERAEAQRRSLLVD
                     RLGGIAYDLSRRGARLLVGTDCGNPFVLAGPSYHKELAELSRSGLDFATVLKAATVDAY
                     AATGRDPARADLVLYRRSPAHDVTRLARPDAVLVDGVLLDADDLDHLWGLRLTAAGLDA
                     GTWRRGDVDPAAGDRQNKETADAG"
     gene            11755..12894
                     /gene="bstI"
     CDS             11755..12894
                     /codon_start=1
                     /gene="bstI"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007:cytochrome P450 (Score: 230.9; E-value: 4e-70)"
                     /gene_kind="biosynthetic-additional"
                     /product="cytochrome P450"
                     /protein_id="AFU90398.1"
                     /sec_met_domain="p450 (E-value: 1.1e-11, bitscore: 33.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFEFFNGPSAYREAAATHADEGAFYSASGDGFWVLTTYEGICEA
                     FRDEEQFTVGRVSAADGAEEDRWIPLTIEGRQHTEWRRRLGAWFTPQRVRELTPSIEAN
                     ARRRIEGFVDDGEVSFNEDFARPFVLENLMTAVGWEFDRFDQLLRIDRAMIDSRYAPDP
                     REAAFEEVGIPALEKLAREQVERRRAEPADDLTTATFGWEVEGRPVTDDDRASLLCVLF
                     LAGVDSTVNHLANAVQHLVCHDADKRRFLASREVRPRAVEEFLRANSCMYPGRLAARDG
                     AGGTARRGETVLLPLAVANYDPEVFHDPEQVDFDRERNPHIAFGTGAHQCLGAALARAQ
                     IRTALDVWHELIPHYGFPPQPEPSEPRFLRNAYDLRLVW"
     gene            12902..>14745
                     /gene="bstJ"
     CDS             12902..>14745
                     /codon_start=1
                     /gene="bstJ"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bottromycin_Methyltransferase_RRE"
                     /gene_functions="other (smcogs) SMCOG1277:Radical SAM
                     domain protein (Score: 158.8; E-value: 3.2e-48)"
                     /gene_kind="biosynthetic-additional"
                     /product="radical SAM-dependent protein"
                     /protein_id="AFU90397.1"
                     /sec_met_domain="TIGR03975 (E-value: 6.2e-163, bitscore:
                     532.8, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="Bottromycin_Methyltransferase_RRE
                     (E-value: 3.2e-42, bitscore: 132.2, seeds: 12, tool:
                     rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSAGSAGRVTLVSMPWNNVRRPSIQVGTLRAVAEEEGWQVTPEY
                     AYLAFYGLAKRMLGYGDAEWSDAYDLISEGLYHFSVGDWIFACRGTEAARRDEYVEELR
                     SRKIDEPTIGLIDSLREVAERHVEETAAALLASDPDVIGFTSTFSQNGPTLAVAERLRA
                     SGYPGVIVMGGSNCEGSMGRALLENYPAVEAVVDGPGEDAFVALLRQVAAGGPVRSQGR
                     LLTRRPAEDSSDVGEAKISPTGLSVPVPNYDGFFEQLHAAGLEGLEAEITLPVEFSRGC
                     WWGAKTHCTFCGLNGATMAYRSKDPDSVADELQYLMDRYGVFDFFAVDNILDLKYLDTA
                     LPLVEKLNTDHSLFFEVKANMEWADIRSMRRAGVRSVQPGIESLSTEGLRLLKKGSTAF
                     QNVRFLLGCAEFGVLPVWNILTGFPKETPELMREQIDLLPSLTHLPPPDNDVLAVHFDR
                     FSPYVESPESFGLSLSKPLPGYRYVYPELSGEDLWNIAYHFEGDFHDDSRNVDIRRRLA
                     ARVRLWRNHHERAHFTYRLGFDSLTLSDHRPGLPAHETLLQGDDAALFRAVIGGTRIRD
                     LQANGWPEGQSWDRAWEKLQNWQRRRWIFIEGTKIIALA"
ORIGIN
        1 ttactgaccc gcaactgccg gatccgtcgg ctcgttcccc acggcctcct ccggctcctg
       61 ttgccggact ttgtcgagct ggcgcagtgc cggaagggcc agcaccgaga cggcgacgac
      121 ggcccagccg agcccgagga gcgcaccggt ctggcgcagc ccgccccact cgatcaggag
      181 gctggcgccg agggtgccga gcgggatcgc cgtcatggag accgcgttct gaagccccag
      241 gacccgacct cgcacgctct ccggaatccg gtccgtgacc aggacgaggc agagggcggg
      301 cagtggccct gcaccgattc ctgcgactac ggccgccccc agcagaatcg gaatcggcgg
      361 gagcagggcg agccccacga tgcccgctgt ggagaggagt tgggcgacga cgaagaccgc
      421 gcgccgcgac aggcgcgcac cgatggcggc gtacagaccg gaaccgacga tgccgcccac
      481 cgcgaggaag gagatcacca gaccgaagcc gccgggcgaa tccttgccca cgaggtacac
      541 gggcaggacg aggttctgca acggcgcgag gatggcggcg ctccccgcgg agacgaaggt
      601 gaccgcggtg acgacgcggt cgtgccgcag cacggagaac ccgcgggcga tgtcggcctt
      661 cagcccgcca ccctcggaag cgggcgcgga cgcggaccgc gctccgaccg tcctgggaag
      721 actcagcgtg acgagggccg cggcggcgga gcaccccgag gtgatccaca gcaccgtggc
      781 cgggtcgacg aggacgatga gcccgcccgc caccgcagga ccgacgacca ccaccacgcc
      841 gccgacgctc tccctgagcc cggccagccg ctcgatgccg accccggagg cggccgcgag
      901 gtcgggcgcc aacgtctccc gggccgtcat tcccggcacg tcgaagagcg ccccggcgac
      961 tcccaggacg acgaaccaca caaccgtcag accggagaag tggtcgatga tcggcagtgc
     1021 ggccacgcag gcggcggagg cgaagtccgc gcccaccgag aggcgtcggc ggccgacgcg
     1081 gtcgatgagc acccctccgg ccacggcgga caccagcatg gggagcgcgc ttgccgcggc
     1141 gacgaggccg accgctgccg cgtcgccggt ccgcagatag accagccacg gaagggcaat
     1201 ggtcgccacg gtgtttccga gcagggacaa cccgtgggag agaaggtatc gggtcagcat
     1261 cggctcagta tggcaccgcg gtaccgggat aacagcgaaa ttttcggcgt catcgctgca
     1321 ttccgcgagg gaccgtatag gtgtggcact cctgctgtcg ctttacgagt tgacgcaccc
     1381 cgaaaaggcg agactgttcg ctcgtaatcg cgttccttgc aggtactcct gttcaacctt
     1441 ctttcgaatc tgctcagttg ggagcggggg cgcagggtgg tgagcatcta tgcccttctt
     1501 gaagccgatt ccttcttcgg tcagccgaag gcgtatcggg aaaggtggcc gacccgtccc
     1561 aacacgggca accgggcgag cacttcggca tcgaccctca tccgtgtgcg aagggcgaac
     1621 ggcagcctcc ggaagtcggc ggggtcggcc gtgtggtaag ccttgaccga cttcaccagt
     1681 cggagccctg ggacctcgcg ctccaactcc cgcggatcgt ccacgcccca ggccaacctc
     1741 gcgccggtct tgcggaagag cgccatggag cgcgtggacc gcacggcgta cctggcgtag
     1801 gtgtcgaaga cggcctcgcc ctcggagaag tgctccacga gagcgcgcag catgcgcggg
     1861 ccctcgtccg ccgccaggta catggtgagg ccctcggcga tcacgaggac cgggaggtcc
     1921 gtggggactt cggcgagcca gtcggtctcc gtcaccgacg cggcgatcgt ccggtggtcg
     1981 ctccgcgggg agaagagctg ctcgcgcacc tcgatgacct cgggctggtc cacgtcgaac
     2041 cagcgcactc cgggtccccg ctcgacgcga tcgacccggt tgtcgaggcc gcaacccagg
     2101 tgcagcacgg tggagttcgg gtgcgcggtc aggaactcgc gggtgcggcg gtcgaagaac
     2161 cgggcacgca gggcgactcc gaccgcgttg gacggccgca tccccagggc gccgaagtcg
     2221 tattcgatct tctgcaccag ttccggcgcc gtcgagtcgg cgagcatcgg gtgcggcgaa
     2281 ctcgcgtcca gcgcgcgggc gtagaggggt gcgaggaggg tcgcggcggc accggtcagc
     2341 gggaccttca tacccggctc cggtgcccgg cgccgtcgac cgtgtgcagg tcctgttggg
     2401 gaccggatac ctcgatggcc gggtcaacca tggccttcgc ctttccgtaa gggccgacca
     2461 aaagggaagc agtgtacgac ttgaggatct ggaaaccgag atcgtcaaga gattccctga
     2521 tcctaggggt acccctgttt tctgcgcaag gaccgggtga tctccgaccg actcgtcaac
     2581 tattcagtaa cgtggccgcc gtgtaagaga gttgagtggc agttgtccta ggcgcctgcg
     2641 atgacctgca ttgacaatgc cttcgatctt cactagctgt ggttctccgg tttttatcga
     2701 tcttcacgtt gcgcctgcgt gccccctgca caatattcga actgcaacga aaaatactgg
     2761 ccaagacggc cggaaccagc tctgctgtac acggctcgaa aatttgatca gcaattgccc
     2821 gacgtatcga ctcttgacat ggatagccgg cgttcagcac tctgcttcag agtccttgcc
     2881 accagctctc gggggagatg attccgtggg caacggccgt agtgccacgc tggtttcgat
     2941 gccgtggaac agtgtggtgc gtccgtcgat tcaggtgggc acgctgcgta cggtggccga
     3001 agaggaaggc tggcaggtca cctccgaata cgcctatctc agtttctacg gcctgggcag
     3061 ggagatgctc gggttcagcg agagcgagtg ggccgacgcc tacgaaacga tctcggaaaa
     3121 gctttaccac atggcggtcg gcgactggat attcgcgcgc cgccacggcg aatcccagca
     3181 acgcgaccgg tacttcgccg agttgcgcga gatgcgggtc gacgacgaaa cgatagcgct
     3241 gatcgattcg ttgcgcccgg tcgccgagcg gcatgtggag gagaccgcgg cggccctgct
     3301 cgcatccgac cccgatgtca tcggattcac ttcgatgttc agccagaacg ggccgacgct
     3361 cgcggtcgcc gagcgtctgc gtgcggaggg ctatccgggc gtgatcgtgc tggggggcag
     3421 caactgcgag ggctcgatgg gacgagccct gctcgcgaac tacccgaccg tggaggccgt
     3481 ggtcgacggt ccgggtgagg atgcgttcgt ggcgctgctg cggcaggtgg cggccggcgg
     3541 ccccgtcagg tcgcaggggc ggctgctcac ccgccgtccg gcggaggatt cttcggacgt
     3601 gggcgaggcg aagatctcgc ccaccgggct cagtgttccg gtgccgaact acgacggttt
     3661 cttcgagcag ttgcatgcgg cgggtttgga gggtctggaa gctgagatca cgttgccggt
     3721 ggagttttcg cggggttgtt ggtggggtgc gaagacgcac tgtacgttct gtggtttgaa
     3781 cggtgcgacg atggcgtacc gcagcaagga tcctgattcg gtggcggatg agttgcagta
     3841 tctgatggat cgctacgggg tgttcgactt tttcgcggtc gataacatcc tcgatctgaa
     3901 gtatctggat acggcgcttc cgttggtgga gaaattgaac acggatcact ctttgttctt
     3961 cgaggtgaag gccaacatgg agtgggcgga catcaggagc atgcgtcgtg cgggagtgcg
     4021 ttccgttcaa cccggcatcg agagcctgag cacggagggt ctacgtctgc tgaagaaggg
     4081 ttccacggcg ttccagaacg tacgcttcct gctcggctgc gccgaatacg gagtgcgggc
     4141 cgactggaac atcctcaccg ggtacccgaa ggagacgccc gaatccatca gggcgcagct
     4201 cgacatcgtc gcctccctct cccatctgac gccgccacac atcacgttga tccggttcga
     4261 ccggttcagt ccgtacgtcg agtccccgga gtcgttcggt ctttcgctgt cgaagcctct
     4321 tccggggtat cggtacgtgt acccggagct gtccggcgag gacttgtgga acatcgctta
     4381 ccacttcgaa ggcgacttcc acgacgacgt ccgcaacgtc gacatccggc gtcgtctcgc
     4441 tgcccgtgtc cgtctgtggc gcaaccacca tgaacaggcc cgtttcacgt acaggttggg
     4501 gttcgactcc ctcactctga gcgatcaccg ccccggcctc cctgcacacg aaaccgtcct
     4561 gcaaggcgac gacgccgcac tcttccgggc tgtcatcggc gggacccgca tccgtgacct
     4621 gcaagccaac ggttggccgg aaggccaacc ctgggaccgg gcctgggaga aactccagaa
     4681 ctggcaacgc cgccgctgga tcttcatcga aggcaccaaa atcatcgccc tcgccatccg
     4741 cgaacaacca tccgcctacc ggacgccgcc gcccaagggc acacccaggc gacaggcccg
     4801 caaccccgtg cccgtcacgc tcaccagccg gaagtgaggg aggtgaggcc tatcgcggtg
     4861 cattcacatg aacagcgcga cgacggatgg atcggtacag cgaaaagaaa ggatcactga
     4921 catgggacca gcggtcgtat tcgactgtat gacggcggac ttcctcaacg acgaccccaa
     4981 caacgccgag ctcagctctc tggagatgga ggaactcgag tcgtggggcg cctggtccga
     5041 cgacaccgac cagtcagtct gatgcccgcc tgcgcgtagc accagacgtg aagtcggcgg
     5101 gccggcgcac agggaagtgg gagtgatggc cgattcttcc gcgaccgact gcgtactgcg
     5161 ggaagtcgcg ttccgctcct accgggcgga gcacacggtg acggtccagt gcacggtacg
     5221 gcctgactcg ggaccggggc aggccgaagg ctacggcacc gccacgaccg aggccgtcgc
     5281 tcggaagaag gccctttccg aagccgtgga aaggctgttg gcctgcaccc cgttcgccct
     5341 catcgcccgt cggccgacac cgcgcacaga ccggagcagg ggcaccgacc cctggcagcg
     5401 cgggctgcgc tccgcgcccg ccggctgcca ggtacgcaac taccgctcgc tgagcggcga
     5461 gcagccgcgg caggtgcccc tgttctggtc atcgccctgg gtcgccggcg aggagatgcg
     5521 gtcggcgaac ctcgccccgc aggcggcccg cctctccagc acggtcgggt gggccgtcgc
     5581 accgtcgccc gaagcagccc tgcagggcgc cctctacgag ctgacggagc tgctgaacta
     5641 cggcgcgttc ctctaccggt gcctcgcggc cccccgcgaa cgccccgcgc ccggcggagg
     5701 ccccgagccg cgcgaagagg gccgcatcca gaccgtcccc ataagctggg cgacccgcac
     5761 cccgacgatc ctcgccgtga cgaggagcag cgaccggctg atgcccgcca ccggtatcgg
     5821 ctcaggtacg acggcgtccg aggcggccga gcgggccttc ctcgaactcg cccaggccga
     5881 gacgctctgg cgcgccaact cgaccgccgt gccggccgag cgctacttca tgcggcgctt
     5941 cgagaagtgg cccctgctga gacgctgcgc cgccctggac ttcgacctcg gcgagcacgc
     6001 cggccccttc gacgacgggc cccacccctc tcccttgggc gcgctggagt ccagagccat
     6061 cgaggtgtgg gccgaccccg gagccgtcga catctcgggc gccgacctga ggaggacccg
     6121 cctgtgcttc gcccacgtgg tgtcggatcc gcagccgctc ctcggccttg tccgcgcagg
     6181 tattcccgtc ttcgacacgg gggaagtgag gaagatcctt gtctgtccac gccgagcacg
     6241 atctgctgac cgcagccctt ctggacgggt cgctcactca ggacgtccca cccgagcgtg
     6301 agacgccgat cggcagagcg ctctccgcgg cgagcgactg gctgagctcc gagcaactga
     6361 ccccggacat ctggaagtac gggctcgact ccgcgcccac ctacgaggtc cacctcaagg
     6421 acgccgcggg cacgctgtgc tcgcgcagct cgggcaaggg tctggggcac cgcagcatgg
     6481 cgagcgccct cttcgaaagc gccgagcact accacctcga ctggcgccgc gacccgctcg
     6541 ccgacgaggc cgcgttcgtc cccggccgcg aggtcgccgg gcagtcgacg gccgccggct
     6601 cggcgctgct gcaccgactc ggcggcattc tcggcgactc cccggtcctc acccgcgaat
     6661 accgcccagt ggccgaggcg ttgggcgcca ccggcggcgc cgcacccccg caccgccacc
     6721 ccgtcttcct gcgcgacggc ggctaccgga actggccgca cccctcggac gacggctcct
     6781 tcaagccgct ctggcactac acgacgagcg ccggctacgc ggccggaacc acgctgcacg
     6841 aggcgctcgt ccacgcggtc aacgagctga tcgagcgcga cgcctggtcc taccaacttg
     6901 cccgttccta cttcggcctg cccgacgaga gcggccgggc cctccgcgtc atcgaccacg
     6961 agagcctccc ggcggagctg cgcgccctcg tcgacgacgt ggaggcgtcg agcggctccc
     7021 cgatcctcat cgtctcgatc ccctgcgaca cgggggtgcc ggcctacgtg gtctgcgacg
     7081 cggagagcga cgaggacgtg cggctcatcg gcagcggcgc ctcgccggtg caggcgtacg
     7141 ccctccaacg agggctgctg gagtacctcc aggtgtgcac gatgtaccag cacgggcccg
     7201 tcgacgcgga gcgcgaggcc gcccagatcg ccacggccct ggcccgctac ccgaggcacc
     7261 tcgccgccgc cgggttcgac gtgcgtcaac tcccgcacga acggcagccg ttcgacagcg
     7321 acgggctccc tgccgcggcg actccggaag agctgctgcg gcacctcatc gaccggttgc
     7381 agggagtggg cgtcgacctc cacttccgcg tcctgacgag gccggggacg atcaccgtcg
     7441 tcgacgtcgt cgcacccggg ctcgaaatgc tggacaaggc gcgcgccggc tacccggtcc
     7501 tgccgaccgg gcggttgggc gagaggctca ggccgcagag gagggagccg tgaagctcgt
     7561 ccttgtggcg ccaccgtgga acagcctcta ccggccctcg atccagatcg ccaccctcgc
     7621 cgcgctgagc accgaggagc agggcgatcc gatcagcgcc gtctacgggt acttggactg
     7681 gttcgagtac gcggtcggct tcctggagtg cgacccggag cagttcgccg ccgtgtacga
     7741 cacgatcggc gaggagctgt acgggctcgg cgtcggcgac tggatcttcg gcggcgggct
     7801 gccgggcgcc ggggacgcgg acgaggcgtc ggcgaccgcg gagtacggtg cgttcctgcg
     7861 ggagcagggc gtcgacgagg aaatcgtcgc cgatgtcctg cggttgcgcg aggcggcgcc
     7921 acggttcgtc gccgacctcg ccgagcggct cgtggacgac ggggccgagt gcttcggatt
     7981 caccacatcc ttcagtcagt tggtgccctc gctggcgata gccgtggcga tcaaggaacg
     8041 ggcgccggac cggctcgtcc tcctcggcgg ggcgaactgc gaccgtccga tgggccaggc
     8101 cgtgatgcac gcctatccgt tcgtcgacgc ggtcgtcgag ggcgagggcg agcaggcggt
     8161 gaacgcgctc acccgcgcga agagcgtgga cgacctggcg gcggtccccg ggatagtgct
     8221 gcggcacgag gacggcctgc gcaccgcgct gccgacgatc caacccgtgg agtccgctcc
     8281 cgtgcccaag tacgacgcgt acttcgagcg gttggagagc ctgccgtggg gcgacctcat
     8341 cgctccgcac gtggcgctgc cgttccagat gtcccgcggg tgctggtggg gcgagaagtt
     8401 gcagtgcacg ttctgcggtg agaacggcag cgggatggtc taccgcagca aaccgggctc
     8461 ccaactcgtc gacacgctgc gcacgttgac ggagcggcac ggcgccttca acacctacgc
     8521 cgtggacacg atcctggcga agaacgacaa cggcctcgcg gcgctcgccg acgccgaggt
     8581 cgacatcacc accttcttcg aggtcaaggc taacctcggc cctcgggagg tcggcgagct
     8641 gcgcgccgcc ggcgtcacca tggtgcagcc ggggatcgag agcctcagct cgggcgcgtt
     8701 gaagctgctg cgcaagggcg cgacgagctg gcacaacctg cgcttcctcg tcctgtgcca
     8761 ggagttgggg atcgacgcgc agtggaacct gctctcggcc atgccggacg agtcagcgga
     8821 gatgctggag gagcagctcc ggctgatccc cttcatcaca catctggcac caccgtcgac
     8881 ggtgagcaag gtccgcgtcg accgctacag cccctacttc gagaagcccg acgagttcgg
     8941 catcgagctg cggggccccg agaagaagta ccggttcatc caccggggcg agggcgtcga
     9001 ccgtacggcg ctggcgtact ccttcgagca cgggcagcag gcggggaagg agcgggacga
     9061 tgacgcagcc atgctggcgc tgcgccgccg gctcggggca cgtgtcggac tgtggaagaa
     9121 gctgcacgcg cagtcccgct tctccttccg tttcggcccg aacgtgaccg tggtccacga
     9181 ctacaggccg cacgtcggca cgggccgcac cgtgctgcgg ggagccgacg acctcctctt
     9241 ccgctccctc gtccacggcg ggcgcctcat gcaggccgtc ggccgggtcg gcgagcagac
     9301 ggggacgccg gagagccggc tgcacgagag ggtgagcgag tgggcggcgt gcggctgggt
     9361 ccatcttgag gggcggcgcg gtctcgtcct cgccgtacgg gacggcatgc gggacgagtt
     9421 gggccgcgcg gcggcgctga gggggtcgga tgcggagtcc tgaccgggag cagacggaac
     9481 gcttcgaagt cgcctccgtg gacggcacgt tgctgcgcgg gttcagccgg gcggcccgcg
     9541 ggtcgtccga tgcgcggggc gagccggaaa cgctggtgct gctgcacggt gtcgcgatgg
     9601 accaccgggt ctggtccgag tcgtcgttcc tcgacgcgct gcccgacgac gtccacgtcg
     9661 tcgcccccga cctgcgcggc cgcggcgaaa gcgacccggg gcacagcgcc gagggccacg
     9721 cgatcgaccg ctacgtcgag gacgtccgcg ccgtgctcgg cttcttcggg cgggagcggt
     9781 acagcctctt cggcctctac ttcgggggcc gtatcgcgct gcgggtcgcg gaggcggaca
     9841 ggcgggtgca gcgggccttc tccttctgcg cgcacggcga ggcggtggag gtcccggcgg
     9901 acgccgtcga ggaggaggcg aaagagatcg aaggccccgg cgggtacgcg tacctccgcg
     9961 accacttctc cgccgcggga gcgcctgcgt ggatggtctc cgcctgcgac cgggtcgacc
    10021 aggacgagtt ggcggcggcg acccgcggcc tcctgcacgg ttcgcaccag cgggccgagc
    10081 gcggagcagc cgaccaggac ctggtgctga tcactgccga gggcgacgcc gacctcggca
    10141 cgttccgcga gggctcggcc cgcctcggcg cccggctctg gctggtggac agccggaccc
    10201 gggtgcgcgc cgctgcccgc ctcgccgaag tcggcacgcg cacggcggag ttgctcaccg
    10261 cggcgccggc ggcgatgaaa ggggacgcat gacggacgac gcccctctgg acccaccgca
    10321 ggggcctgct ccccgacggc gatccgctcg tcggcgcgct ggaggagctg gcgggccgca
    10381 gccgcagcca cgtcctccgc gccgtcggga tgctcgacgc ggagaccgga gaggccgccc
    10441 cgccaaggga cttggaggtg gtcgacggcg ccgtcgcccg ctggagccct gcgccggacg
    10501 ccgcgtactg cgagtggttc gccctgcccg ggttcgtcga cgcccatgcg cacgtgtcct
    10561 cggtgaccga cctcgtcggc atgctcgcgt acggggtgac gggctaccgg cagatgtggg
    10621 gcgagcccgc acacctgtac agcgccggcg tccaccgtgc gcgccacgcc gtcctccccc
    10681 tcccctggac gacggcgggc gtcgtcgacg gccccgactc ccgtatcccg caccacgtca
    10741 cggtcgttga cgacgcgcgg caggtgcgcc gggtggtcga ggacgtcctc gcgttcggct
    10801 tcgacggcat caaggtctac gacgacttgg cgcggccggc cttcgaggcg ctggtacgcg
    10861 aggcggagcg cgccggcatc cccgtcgtcg gacacgtgcc cgaggcggtg ccgctcgacg
    10921 cggcctaccc gggcatgtgg tcgaccgagc acctgtacgg aatcgtgccc aacgtcttcc
    10981 ggctgcccgc ggccgaccgc tgggccgtcc tcgccgacgc gttgcagcgg caccgcgacg
    11041 acggccccgc cgtcgagggg gccgccggcc gtttcgtctg cccgacgctc accgcgtggc
    11101 gggcgcggag cggggagcgc cgctacaccc ggccgtcgag gacgctcctc cagtcggcga
    11161 ccccgagccg ccgccccgcc tggcacgcgg ccgcgaggga cgccctctcg ctggagcggg
    11221 ccgaggcgca gcggcggagc ctgctggtcg accggctcgg aggcatcgcg tacgacctgt
    11281 cgcgccgcgg ggccaggctg ctcgtcggca ccgactgcgg gaaccccttc gtcctcgccg
    11341 ggccctcgta ccacaaggag ctggccgaac tcagccgcag cggcctcgac ttcgcgaccg
    11401 tcctcaaggc cgcgaccgtc gacgcatacg ccgcaacggg ccgcgacccc gcacgggccg
    11461 acctggtcct ctaccggcgc tccccggcgc acgacgtcac ccgcctggcc cggccggacg
    11521 ccgtactcgt cgacggagtc ctcctcgacg ccgacgactt ggaccacctg tgggggctgc
    11581 ggctcacggc ggccggcctc gacgccggca cctggagaag gggcgacgtg gacccggcgg
    11641 ccggtgaccg acagaataag gagactgccg atgcaggctg acccatccac cgggacgacc
    11701 gaccccgagg ctccccccgg ggtctgcccc gtcaccggac ggcgcgccga ccccgtggac
    11761 ttcgagttct tcaacggccc ttcggcgtac cgcgaagcag cggccaccca cgccgacgag
    11821 ggcgccttct acagcgccag cggcgacggg ttctgggtcc ttaccacgta cgagggaatc
    11881 tgcgaggcgt tcagggacga ggagcagttc accgtcgggc gcgtcagcgc agcggacggc
    11941 gctgaggagg accgctggat tccgctgacc atcgagggcc gccagcacac ggagtggcgc
    12001 cgcaggctgg gcgcgtggtt cacgccgcag cgcgtacgcg agctgacccc ctcgatcgag
    12061 gccaacgccc ggcgccggat cgaggggttc gtcgacgacg gcgaggtgtc gttcaacgag
    12121 gatttcgcca ggccgttcgt gctggagaac ctgatgaccg cggtgggctg ggagttcgac
    12181 cgcttcgacc aactcctgcg catcgaccgc gcgatgatcg attcgcggta cgcgcccgac
    12241 ccgcgtgagg cggcgttcga agaggtcggc atcccggcgc tggaaaagct cgcccgcgag
    12301 caggtcgagc gccgtcgcgc cgaacccgca gacgacctca ccaccgcgac cttcggctgg
    12361 gaggtggagg ggaggcccgt caccgacgac gaccgggcct cgctgctgtg cgtgctcttc
    12421 ctcgccggcg tcgactcgac ggtcaaccac ctggccaacg ccgtccagca cctggtgtgc
    12481 cacgacgccg acaagcggcg gttcctggcc tccagggaag tacgcccgcg ggcggtcgag
    12541 gagttcctcc gggccaactc gtgcatgtat ccggggaggt tggccgcgcg cgacggggcg
    12601 ggcggcaccg cccggcgggg tgagacggtg ctgctgccgc tcgccgtcgc caactacgat
    12661 cccgaggtct tccacgaccc cgagcaggtc gacttcgacc gggagcgcaa cccgcacatc
    12721 gccttcggta caggagcaca ccagtgcctc ggcgccgcgt tggcgcgggc gcagatccgc
    12781 accgccctcg acgtgtggca cgagctcatc ccgcactacg gattccctcc gcagccggag
    12841 ccctcggagc cccggttcct ccgcaatgca tacgacttga ggctcgtctg gtgaggtcgc
    12901 catgaagagt gccggcagtg ctggtcgagt aacgctcgtt tcgatgccgt ggaacaacgt
    12961 gcgccgccct tcgatccagg tgggcacgtt gcgtgcggtg gccgaggagg aaggctggca
    13021 ggtcaccccg gaatacgcct acctcgcctt ctacggactc gccaaaagga tgctgggcta
    13081 cggagacgcc gagtggtcgg acgcgtacga cctgatctcc gagggccttt accacttctc
    13141 ggtcggcgac tggatattcg cctgccgggg cacggaggcc gcgcggcgcg acgagtacgt
    13201 cgaggagctg cgctccagga aaatcgacga gccgacgatc gggctgatcg attcgctgcg
    13261 cgaggtcgcc gagcggcatg tggaggagac cgccgcggcc ctgctcgcgt ccgaccccga
    13321 tgtcatcggg ttcacgagca ccttcagcca gaacgggccg acgctggcgg tggccgagcg
    13381 gttgcgtgca agtggctatc ccggcgtgat cgtcatgggc ggtagcaact gcgagggctc
    13441 gatggggcgg gcgcttctgg agaactaccc cgcggtggag gcggtggtcg acggtccggg
    13501 tgaggatgcg ttcgtggcgc tgctgcggca ggtggcggcc ggcggccccg tcaggtcgca
    13561 ggggcggctg ctcacccgcc gtccggcgga ggattcttcg gacgtgggcg aggcgaagat
    13621 ctcgcccacc gggctcagtg ttccggtgcc gaactacgac ggtttcttcg agcagttgca
    13681 tgcggcgggt ttggagggtc tggaagctga gatcacgttg ccggtggagt tttcgcgggg
    13741 ttgttggtgg ggtgcgaaga cgcactgtac gttctgtggt ttgaacggtg cgacgatggc
    13801 gtaccgcagc aaggatcctg attcggtggc ggatgagttg cagtatctga tggatcgcta
    13861 cggggtgttc gactttttcg cggtcgataa catcctcgat ctgaagtatc tggatacggc
    13921 gcttccgttg gtggagaaat tgaacacgga tcactctttg ttcttcgagg tgaaggccaa
    13981 catggagtgg gcggacatca ggagcatgcg tcgtgcggga gtgcgttccg ttcaacccgg
    14041 catcgagagc ctgagcacgg agggtctacg tctgctgaag aagggttcca cggcgttcca
    14101 gaacgtacgc ttcctcctcg gctgcgccga attcggcgtc ctccccgtgt ggaatatcct
    14161 caccgggttc cccaaagaga caccggagtt gatgcgcgag cagatcgacc tgcttccttc
    14221 gctcacccat ctcccgccgc ccgacaacga cgtcctcgcc gttcacttcg accggttcag
    14281 tccgtacgtc gagtccccgg agtcgttcgg tctttcgctg tcgaagcctc ttccggggta
    14341 ccggtatgtg tacccggagc tgtccggcga ggacttgtgg aacatcgctt accacttcga
    14401 gggcgacttc cacgacgact cccgcaacgt cgacatccgc cgtcgtctcg ctgcccgtgt
    14461 ccgcttgtgg cgcaaccacc atgaacgggc ccacttcacg tacaggttgg ggttcgactc
    14521 cctcactctc agcgatcacc gccccggcct ccctgcacac gaaacccttc tccaaggcga
    14581 cgacgccgcg cttttccggg ctgtcatcgg cgggacccgc atccgtgacc tgcaagccaa
    14641 cggttggccg gaaggccaat cctgggaccg ggcgtgggag aaactccaga actggcaacg
    14701 ccgccgctgg atcttcatcg aaggcaccaa aatcatcgcc ctcgc
//
