LOCUS       BGC0000471             12970 bp    DNA     linear   BCT 11-SEP-2019
DEFINITION  Oscillatoria nigro-viridis PCC 7112 chromosome, complete genome.
ACCESSION   BGC0000471
VERSION     BGC0000471
KEYWORDS    .
SOURCE      Oscillatoria nigro-viridis PCC 7112
  ORGANISM  Oscillatoria nigro-viridis PCC 7112
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Oscillatoriales; Oscillatoriaceae; Oscillatoria.
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 4087498
            Source DNA and Organism available from Cheryl A. Kerfeld
            (CKerfeld@lbl.gov)
            Contacts: Cheryl A. Kerfeld (CKerfeld@lbl.gov)
            Tanja Woyke (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:42:15
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 4308239
            Orig. end    :: 4321209
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..2001
                     /codon_start=1
                     /db_xref="InterPro:IPR000209"
                     /db_xref="InterPro:IPR015500"
                     /db_xref="InterPro:IPR022398"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 177.1; E-value: 1.2e-53)"
                     /gene_kind="biosynthetic"
                     /locus_tag="Osc7112_3648"
                     /note="PFAM: Subtilase family; TIGRFAM: cyanobactin
                     maturation protease, PatA/PatG family; COGs: COG1404
                     Subtilisin-like serine protease; InterPro IPR000209; KEGG:
                     cyj:Cyan7822_6539 peptidase S8 and S53 subtilisin kexin
                     sedolisin; PFAM: Peptidase S8/S53,
                     subtilisin/kexin/sedolisin; SPTR: Putative uncharacterized
                     protein"
                     /product="peptidase S8 and S53 subtilisin kexin sedolisin"
                     /protein_id="AFZ08002.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 8e-39, bitscore:
                     122.7, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 3.5e-233,
                     bitscore: 763.3, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDTLSTVSQGDSSICIAVLDGPVDQTHPCFQGADLTYLPTLVKG
                     EAQIGGNMSAHGTHVASIIFGQPGSPVEGIAPKCKGIIVPVFADDRLKTSQLDLARAIE
                     QAVNAGAHVINLSGGQLTDYGEADGWLQNAVRLCRENNVLLVAAAGNNSCDCLHVPAAL
                     PSVLAVGAMDADGKPLDFSNWGEAYKDQGILAPGKDILGAKPGGGTDRLSGTSFATPIV
                     TGVAAVLLSLQREQGETPDPQKIRQVLLQSALPCDPDLPEETRRCLAGRLNIPGAITLL
                     KKGGNMSENFASVGASEVEAAGCGCGGGLTNASETTAAADRAQPAPSLVAAGAKPGAAE
                     AFPSNTPNLQNLMQSFPTPVTQPPMMPNFTANSVTASQAPSELPELGQIVYALGTLGYD
                     FGSEARRDSFKQLMPPFDIGGGVMVPANPYDARQMVDYLGENISEARSLIWTLNIELTP
                     VYAIDPTGPFGAESYRALHELLSGQIQPENDAEYVERVSIPGILTGRKVKLFSGQVVPV
                     IEPQTTRGIYGWKVNSLVSAAMDAVQAEEGTADEDRIRNTLDGFLNRIYYDLRNLGITS
                     QDRALNFSVTNAFQAARTFSEAVAVGMELDSVTVEKSPFCRLDSDCWDVKLKFFDPENN
                     RRAKKIFRFTIDVSDLIPVTLGEVRSWSSPY"
     subregion       1..12970
                     /aStool="mibig"
                     /contig_edge="False"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12970
                     /contig_edge="False"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..2001
                     /locus_tag="Osc7112_3648"
                     /note="IMG reference gene:2504090191"
     gene            2098..2349
                     /locus_tag="Osc7112_3649"
                     /note="IMG reference gene:2504090192"
     CDS             2098..2349
                     /codon_start=1
                     /locus_tag="Osc7112_3649"
                     /note="KEGG: cyn:Cyan7425_0514 hypothetical protein; SPTR:
                     Putative uncharacterized protein mcaB"
                     /product="hypothetical protein"
                     /protein_id="AFZ08003.1"
                     /transl_table=11
                     /translation="MRLPVLSPPVKRPHFIQPALCVDVEEGLTDDLVHIRMDLLHGANY
                     NDPSAYANRSLNQVMYSSASQSAASQGWGGFAGLGRFY"
     gene            2374..3132
                     /locus_tag="Osc7112_3650"
                     /note="IMG reference gene:2504090193"
     CDS             2374..3132
                     /codon_start=1
                     /db_xref="InterPro:IPR008979"
                     /locus_tag="Osc7112_3650"
                     /note="KEGG: cyj:Cyan7822_6542 hypothetical protein; SPTR:
                     TenC"
                     /product="hypothetical protein"
                     /protein_id="AFZ08004.1"
                     /transl_table=11
                     /translation="MMPQQTETLWQIGQAGKGAQEFSQVGGWQDEFTYVIGSDRDPINQ
                     PQMPSLLVVPGRKTKPKSKGKQLFSTDKLNIQFTLAHSYSTGELTLFYNFFGSQVDTLW
                     VDGEPVTELVGVGSGKPTKTQIPLPALSAGQHTLTLTTAGGSRDGAHRIDYLKLEGVVA
                     PQQPQTLQTPETPKETKETMANEKKETVAKETKEVTPSEVSSPPEEKKSYVPTSQTGLQ
                     DYSYWCDYCKKQAKKAQRHPKPFRRGRIWA"
     gene            3182..5512
                     /locus_tag="Osc7112_3651"
                     /note="IMG reference gene:2504090194"
     CDS             3182..5512
                     /codon_start=1
                     /db_xref="InterPro:IPR003776"
                     /db_xref="InterPro:IPR016040"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cyanobactin_D_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Goadsporin_CD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NHLP_CD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) YcaO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03604"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Osc7112_3651"
                     /note="PFAM: YcaO-like family; TIGRFAM: uncharacterized
                     domain; bacteriocin biosynthesis cyclodehydratase domain;
                     bacteriocin biosynthesis docking scaffold, SagD family;
                     COGs: COG1944 conserved hypothetical protein; InterPro
                     IPR022291:IPR003776; KEGG: ter:Tery_2355 hypothetical
                     protein; PFAM: YcaO-like; SPTR: Putative uncharacterized
                     protein; TIGRFAM: Bacteriocin biosynthesis,
                     cyclodehydratase; YcaO-like"
                     /product="bacteriocin biosynthesis cyclodehydratase domain
                     protein"
                     /protein_id="AFZ08005.1"
                     /sec_met_domain="Cyanobactin_D_RRE (E-value: 1.8e-43,
                     bitscore: 135.7, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="Goadsporin_CD_RRE (E-value: 1.3e-20,
                     bitscore: 62.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="NHLP_CD_RRE (E-value: 8.2e-23, bitscore:
                     69.5, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 3e-97, bitscore: 315.3,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 2.1e-86, bitscore:
                     279.1, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQSTTLLQIKPHFHVEAIEPKQVYLLGEQSNHALTGQLYCQIVPL
                     LNGQHTLEQIVEKLDGEVPAEYIDYVLDRLAEKGYLTEVAPELSSEVAAFWSELGIAPP
                     VAAEGLQQPVMLTAVGDNISEVTVAALGTALRDMGIPVQNPTDADSSAALNIVLTDDYL
                     QPELAAINKQALERQQTWLLVKPVGSVLWLGPVFVPGKTGCWGCLAHRLGGNREVESSV
                     LRQKQAQQQRNGQNGSVLGCLPTARATLPSTLQTGLQFAATEIAKWIVKQHVNATAPGT
                     ARFPTLDGKVITFNQTILDLKSHILIKRPQCPTCGDPKILQRRGFEPLKLESRRKQFTH
                     DGGHRAATPEQTLQKYQHLIGPVTGVVTELVRISDPANPLVHTYRAGHSFGSSTSLRGL
                     RHTLRHKSSGKGKTDSQSRASGLCEAVERYSAIFQGDEPRKWATLAELGDLAIHPEQCL
                     CFSEAQYANRDMLNERATVAHDWIPQRFVPSQAIDWTPMWSLTEQTHKYVPIAMCYYHY
                     PSPPNHHFCRNDSNGNAAGNTLEEAILQGFLELVERDSVALWWYNRLSRPAVDLCSFNE
                     PYFVQLQQFYQESDRDLWVLDLTADLGIPAFVGVSNRKSGSSERLVLGFGAHLDPSIAI
                     LRALTEVNQIGWELDKVPDENLKGEATNWLLTATLADHPYLVPDSTQALKTAQDYPKLW
                     GDDIYTEVMTCVKIAQEAGLETLVVDQTRPDIGLNVVKVIVPGMRHFWSRFGSGRLYDV
                     PVKLGWRDEPLAEAQMNPTPMPF"
     gene            5647..5874
                     /locus_tag="Osc7112_3652"
                     /note="IMG reference gene:2504090195"
     CDS             5647..5874
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_precursor"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: TIGR03678"
                     /gene_kind="biosynthetic"
                     /locus_tag="Osc7112_3652"
                     /note="TIGRFAM: bacteriocin leader peptide,
                     microcyclamide/patellamide family; InterPro IPR022264;
                     KEGG: cyn:Cyan7425_0517 hypothetical protein; SPTR:
                     Microcyclamide protein; TIGRFAM: Microcyclamide/patellamide
                     bacteriocin family, leader peptide"
                     /product="bacteriocin leader peptide,
                     microcyclamide/patellamide family"
                     /protein_id="AFZ08006.1"
                     /sec_met_domain="TIGR03678 (E-value: 6e-25, bitscore: 76.5,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_precursor (E-value: 1.0,
                     bitscore: 1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKKNILPNPGKPVIRGISGKLPSYLAELSEEALGDAGADASFIC
                     SVDASFICSVDASFICSVDASFICSVDGDA"
     gene            6008..7849
                     /locus_tag="Osc7112_3653"
                     /note="IMG reference gene:2504090196"
     CDS             6008..7849
                     /NRPS_PKS="Domain: oMT (96-258). E-value: 8.2e-19. Score:
                     58.8. Matches aSDomain: nrpspksdomains_Osc7112_3653_oMT.1"
                     /codon_start=1
                     /db_xref="InterPro:IPR013216"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 106.2; E-value:
                     3.2e-32)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="Osc7112_3653"
                     /note="PFAM: Methyltransferase domain; COGs: COG2226
                     Methylase involved in ubiquinone/menaquinone biosynthesis;
                     InterPro IPR013216; KEGG: cyj:Cyan7822_6540
                     methyltransferase type 11; PFAM: Methyltransferase type 11;
                     SPTR: Methyltransferase type 11"
                     /product="Methyltransferase type 11"
                     /protein_id="AFZ08007.1"
                     /transl_table=11
                     /translation="MLGKPTFSQVLPSFICLVFFNSTRCIQTHNPAIQILSFRKLSPEN
                     TMSNNYISSSVTTHLNQTPEVEKVRTFYNNHSINFSRMVPDAVARSHNLGLAARMGIQP
                     GNYIFDAGCGAGIPAIHIAQSFPGTRIEGLTISEVEAAEAQTRIAQTNLVDRVRVQVGD
                     FHSPPFPNEVFDVVFFNDSIKYSNQLPQVLAEVKRVLRPGGTLYITGLFVKEPPLSPLE
                     QRALDEINRQELQGSYVIPLKNLVEFVQQAGFQAIEYDENQTVKMPALALRLTAANLMH
                     PYLPLLFADIKAVKPTIVKAKSSIRPSGLTYNHAQNRHQENLRYIEQHKQIFDMENSYL
                     LNLFEGLVGNQRIGKGGSISCCCNIDKDRLYPARFSYLCEVHVSDPGIKLLDIEHFLYF
                     SHRVEERPEIQIDRRLLKQFLATGLDPSKVDMVIFAIDVRPELINSRIKLYLTIKDYPE
                     KIATAISLCGEQRDWENLIINGMLLVGFDFFFDNRAAVEVYPTFYAADLQRPDVQAYLT
                     SRLPAKALPLLHESRCVQIGISNENKSDVLYFNKFKSPNNAVDYLKNQMVSQVHAQYRN
                     RVFHDLYLGIPESEFYTDSIQRVKMYYHMNDFVSRMK"
     aSDomain        6296..6781
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_Osc7112_3653_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="8.20E-19"
                     /label="Osc7112_3653_oMT.1"
                     /locus_tag="Osc7112_3653"
                     /protein_end="258"
                     /protein_start="96"
                     /score="58.8"
                     /tool="antismash"
                     /translation="AARMGIQPGNYIFDAGCGAGIPAIHIAQSFPGTRIEGLTISEVEA
                     AEAQTRIAQTNLVDRVRVQVGDFHSPPFPNEVFDVVFFNDSIKYSNQLPQVLAEVKRVL
                     RPGGTLYITGLFVKEPPLSPLEQRALDEINRQELQGSYVIPLKNLVEFVQQAGFQAIE"
     gene            7937..8899
                     /locus_tag="Osc7112_3654"
                     /note="IMG reference gene:2504090197"
     CDS             7937..8899
                     /codon_start=1
                     /locus_tag="Osc7112_3654"
                     /note="KEGG: mar:MAE_00500 hypothetical protein; SPTR:
                     TenF"
                     /product="hypothetical protein"
                     /protein_id="AFZ08008.1"
                     /transl_table=11
                     /translation="MPITSVFSHNYLREQRLRFIRTHRDAFDVPYSFLLPLYEEAVFKI
                     EGSCGVEPSCNVEGDRLFAGDFQVANYGHTWPRSLIDAASFLDKIERRIDVKINRNLLE
                     RFSALYIGSSKIENNTIGIDLRPTLQDSVIKIYMHIHPGQSNEDLVMTALALDGAIYSA
                     ELTQVLLRDVVVIGFNLFLDGRSNIEIWAGSAGQKHNHQGNLGRDLTAYIRKYYSHKVN
                     YMFNVSDFSAVSFSKQKIDPLFHFHFFDIKDILKYFAFNSLGDKIYDFCQSQDCITYTG
                     VSARAQELESNRLNNYSFFYNQSDTCQTDLDILRLPMMF"
     gene            9092..12970
                     /locus_tag="Osc7112_3655"
                     /note="IMG reference gene:2504090198"
     CDS             9092..12970
                     /codon_start=1
                     /db_xref="InterPro:IPR000209"
                     /db_xref="InterPro:IPR000415"
                     /db_xref="InterPro:IPR015500"
                     /db_xref="InterPro:IPR022398"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 83.1; E-value: 4e-25)"
                     /gene_kind="biosynthetic"
                     /locus_tag="Osc7112_3655"
                     /note="PFAM: Nitroreductase family; Subtilase family;
                     TIGRFAM: SagB-type dehydrogenase domain; cyanobactin
                     maturation protease, PatA/PatG family; InterPro
                     IPR020051:IPR000209; KEGG: ter:Tery_2365 peptidase S8/S53
                     subtilisin kexin sedolisin; PFAM: Peptidase S8/S53,
                     subtilisin/kexin/sedolisin; SPTR: Similar to A0YXD9_9CYAN
                     Hypothetical protein; TIGRFAM: SagB-type dehydrogenase
                     domain"
                     /product="SagB-type dehydrogenase domain protein"
                     /protein_id="AFZ08009.1"
                     /sec_met_domain="PF00881 (E-value: 1.2e-14, bitscore: 43.8,
                     seeds: 78, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.1e-20, bitscore:
                     63.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 1e-178,
                     bitscore: 583.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYHPFAVSLNPFVQVTSTEDHYTLRSAESDPTALLTMATTPGIG
                     MALDRLKAGNTTPDSLVQGLAELDGRAAGEQFGRMLQQLDERGWLSYAVLPLAVAIPMV
                     DSAELNLTEPYWTQTHLTLSRFAYQHSHEGAMVLESPLSKFRIKLLDWRASAILAQLAQ
                     PQSLATLTPPPYLGPETAYQFLNLLWAAGFLTADPEPPSLQLWEFHNLLFHSRSRLGRH
                     DYPPQDINIKQWSDFPVVKSPMSHQIVPLPRPNLGALKCNDATLTQAIETRKSIREYDE
                     TNPITIEQLSELLYRTARVKEILQTEEWFGENWRQILGFDAAIEYGELGSRPYPGGGAM
                     YELEIYPVVRRCRVLDAGLYHYDSLNHQLERIAESEDVFALQPYIYYKPQPQVLLVITA
                     RFGRLFRKYRSVAYSLVLKHVGALQQNLYLVATNMGLAPCALGSGDSDGFGQAAGLDYV
                     EESAVGEFMLGSLPKRDVQASGVESVQEINLSDEPAELAASESTVESDRGEAEPEPAEV
                     GFRSSTQPTSELDVGFRSSTQATESVETTEVAVSVSEAESVSQTTTQVMDSEASESEVE
                     SDVVVNETESAAISASGVESSATPLSAALHPHDLDDRIPGLAHLRNQTLGDPRITIVVI
                     EGNPDHSLSCFQSAEISKVFPYWEDTPEPISSEEYATFRAIEDSDRTKKEKEESLKAAF
                     PETLIQRLKVDDHTCHVTSIIVGQENTPSAGIAPHCRLINLSLTSGIAEEYISPLNLAR
                     AFEQALDLGANIIHCGICHPTKTGEGEELFAKAVQKCLDNNILIVGPAGNGLGEYWCLP
                     GVLPGTLPVGAMDLGSPPAHYSNWGGNYTEEGIMAPGDDIWGAQLATEKPRLRTGTSGA
                     APIVTGIAALLMSLQLQEGKPVDAEAVRTALLKTAIPCDPKVVEEPERCLRGFVNIGGA
                     MKLLFGRPSFTISFAGDRVTRTERGGYAMATATAAPASIAAAMTDTAVSPALQPATPAT
                     LTEEPVTAAATPADTGTVEASTAFSGNVYALGTIGYDFGDETRRDTFQQQMAPAEVNGI
                     MVPPDPHDPRQMVEHLDRHPDESRSLIWTLSLDGDTIYALESKDGDVYEMFVLMLAGQL
                     EPEASDEFVERISIPARRTKRTVELFSGEVVPVVTVPNVRGMYGWKVNTLVNSAMAAIS
                     RQVDEAQEPLVRQALTAFLNRVYNDLRNVGQTSRDRALNFAATNIFQAAVTFARAIVDR
                     RQLDTINVEKSPFCRINSDCWDVKLVFYDPDNTRRGRKVFRFTLDVVLLMPVTLGQVRS
                     WSLPSKES"
ORIGIN
        1 atgctagata cattaagtac ggtgtcccag ggagatagta gtatttgtat tgccgttttg
       61 gatggtcctg ttgaccagac tcatccttgc ttccaaggcg ccgacttaac gtatctgccc
      121 accttggtta aaggggaggc ccagatcggt ggcaatatgt ccgcccacgg aacccacgtt
      181 gccagcatta tctttgggca accgggcagt ccggtggagg ggatagctcc caaatgtaag
      241 gggatcattg tgccagtctt tgctgacgat cgcctcaaaa cttcccagct agatttggct
      301 agggcgatcg agcaagcagt caatgccggc gcccacgtta tcaacctgag tgggggtcaa
      361 ctgaccgact atggcgaggc cgatggctgg ttacagaatg ccgtccgttt gtgccgagag
      421 aacaacgtct tgctcgtcgc tgcggctggc aacaatagtt gtgattgctt gcacgtaccg
      481 gctgccttgc cctctgtgct ggcggtcggg gcgatggatg ctgatggcaa acccctggac
      541 tttagcaact ggggcgaagc ctacaaagac caaggcatcc tggcccctgg caaggacatc
      601 ctgggagcta aacccggtgg tggcaccgac cgcctgagcg gtacgagctt cgccactccg
      661 attgtcacag gtgtagcagc cgtgctgctg agtcttcagc gggagcaggg agaaaccccc
      721 gacccccaaa aaattcgtca agtgttgttg cagagtgcct taccctgcga ccctgacttg
      781 ccagaagaaa cccgtcggtg tctggcgggc agactgaaca tccctggagc cataacgtta
      841 ttaaaaaaag gaggaaatat gtctgagaat ttcgcatcgg tgggtgcatc agaagtagaa
      901 gcagcaggct gcggttgtgg tggcggctta accaacgcca gtgaaacaac tgccgccgcc
      961 gatcgagcgc aaccggctcc tagtttggtt gctgctggag ccaagccagg agcggctgag
     1021 gctttcccta gcaacacccc caacttgcaa aacttgatgc aatcgtttcc tacacctgta
     1081 acccaaccac caatgatgcc caacttcact gctaactccg tcaccgccag ccaagccccc
     1141 agcgaacttc cggagctcgg tcagattgtt tatgccctag gaaccctcgg ctacgacttc
     1201 ggaagcgaag cccgccggga ctcgttcaag caactgatgc cgccgtttga cataggggga
     1261 ggggtgatgg tgccggcgaa cccctacgac gcccgtcaga tggtggacta cctgggcgag
     1321 aatatttctg aggcgcgatc gctcatttgg acgcttaaca ttgaactgac tcccgtctat
     1381 gcgatcgacc ccaccggtcc cttcggcgcc gagtcctacc gcgctctcca cgaactgcta
     1441 tccggtcaaa tccaacccga aaacgatgcc gaatacgtgg agcgcgtcag cataccgggc
     1501 atcttgaccg ggcgcaaagt caagctcttc tccggtcaag ttgtcccggt aattgaaccc
     1561 caaaccaccc gcggcatcta cggttggaaa gtcaacagct tggtcagcgc agcgatggac
     1621 gcggttcaag ctgaagaggg cactgctgat gaagaccgca tccgcaacac cttagatggc
     1681 ttcctcaacc gcatctacta cgacctccgc aacttgggaa tcacctccca agaccgcgcc
     1741 ctcaacttct ccgtcaccaa cgccttccaa gccgcccgaa ccttcagcga agcggttgcc
     1801 gtcggcatgg aactcgacag cgttactgtt gagaaaagtc ccttctgtcg cctcgatagc
     1861 gactgctggg atgtgaagtt gaagttcttc gaccctgaaa acaaccgccg tgctaagaag
     1921 attttccgct tcaccattga tgtcagcgat ctgattccgg tcacattggg cgaagttcgt
     1981 tcttggtctt caccgtatta aagggcaata caatgggggt gagagtgcta caacaaaatg
     2041 cactctcgcc aagatatcta gatttgttca ccctatccat acggagaatc ctgaaccatg
     2101 cgacttcctg tattgtctcc ccccgtcaaa cggcctcatt tcatccagcc tgcgctgtgc
     2161 gtggatgtgg aagagggtct taccgacgat ttggtgcata ttcggatgga tctgctccat
     2221 ggcgctaact ataacgatcc ttcagcctat gccaaccgca gtttgaacca agtgatgtac
     2281 tctagtgcca gccagagtgc agccagccag ggctggggcg gctttgcggg cctgggacga
     2341 ttttattaat gagccaccga tcggccccta atcatgatgc ctcagcaaac tgaaacccta
     2401 tggcaaattg gtcaagctgg taaaggagct caggagtttt cgcaggtagg cggctggcaa
     2461 gatgaattta cttacgttat tgggtcagat cgagacccga tcaaccagcc ccagatgcca
     2521 tcgttgctcg tggttcccgg tcgcaagacc aaaccaaaga gcaaaggaaa gcagcttttt
     2581 tccaccgaca aactgaatat ccaattcacc cttgcccaca gctacagcac cggcgaactt
     2641 acgctctttt acaacttttt tggctctcaa gttgacaccc tctgggttga tggtgagcca
     2701 gtgaccgagc tcgtcggggt cgggtcaggc aagccaacca aaacccagat tcccttgcca
     2761 gccctcagcg ctgggcaaca taccctgacc ctgaccactg ctggtgggtc gagagatggg
     2821 gcacaccgga tcgactacct caagttagag ggagttgtgg caccccagca accgcaaacc
     2881 ctccaaacac ccgaaacacc caaggaaact aaggaaacta tggctaatga gaaaaaggaa
     2941 acggtggcta aggagactaa agaagtaaca ccctcggaag tctcctcccc tccagaagag
     3001 aaaaagtctt acgtacctac ttcccagacc gggctgcaag attattccta ctggtgcgac
     3061 tactgcaaga agcaagccaa gaaggcacag agacacccta aacccttccg ccggggccgc
     3121 atctgggcct aatccctagc ctgtctagtt atcgtccact taatgactga ctgttcgctc
     3181 tatgcaatcc accacactcc tccaaattaa gccgcacttt catgtcgagg cgatcgagcc
     3241 aaagcaagtc tacctgcttg gcgaacagag caaccacgcc ctcaccgggc aactctactg
     3301 ccaaatcgtg cctctgctga atgggcaaca taccctagag cagattgtcg aaaagcttga
     3361 tggagaagtc ccggcggaat atattgacta tgtgctcgat cgccttgcag agaaaggcta
     3421 cctaaccgag gtagccccgg aactatcctc ggaagtggcg gcattttgga gcgagttagg
     3481 gattgcaccc cccgtagcgg ccgaggggct acagcagccg gtcatgctga cagcggtggg
     3541 cgataacatc agtgaagtaa ctgttgctgc cctgggaact gccctgcggg acatgggcat
     3601 tccggtgcaa aaccccacag acgcggattc ctcagcggcg ctcaacatcg tcttgaccga
     3661 tgactatttg cagccggaac tagcagccat caacaagcag gctctggagc gccaacaaac
     3721 ttggctgctc gtcaagccgg tgggcagtgt cctgtggttg ggcccagtgt ttgttcccgg
     3781 aaagacaggc tgttggggct gtctggctca tcggctgggg ggcaaccgcg aagttgagtc
     3841 gtcggtattg cggcaaaaac aagcccaaca gcagcgcaac ggtcaaaacg gaagtgtatt
     3901 aggctgcttg cccacagcac gggcgacgct gccttcgacc ctgcaaacgg ggttacagtt
     3961 tgccgcgact gagatagcga agtggattgt caagcaacac gtcaatgcca ctgcgccggg
     4021 aacggcacgc ttccccacgc tggacggcaa ggttatcacc ttcaaccaga cgatcctgga
     4081 cttgaaaagt catatcttga ttaagcggcc gcaatgtccg acctgtggtg acccaaaaat
     4141 tttgcagcgg cgagggtttg aaccgctgaa gctagaatcg cgccgcaagc agttcaccca
     4201 cgatggtggt caccgggccg ccactcccga acagactttg cagaaatacc agcacttgat
     4261 cggccccgta acgggagtgg taacggaact ggtgcgaata tctgacccag ctaatccgtt
     4321 ggtacatacc taccgggcgg ggcatagctt cggcagttct acctccctgc gtggtctacg
     4381 ccataccttg cgccataaga gttccggtaa gggtaagacc gatagccaat cacgagcgag
     4441 tggtttgtgt gaggcagtag aacgttattc ggctattttt cagggggacg aaccccgtaa
     4501 atgggcaacc ctcgcagaac taggagattt ggcgattcat ccagagcagt gtttgtgctt
     4561 tagcgaggcg cagtatgcta accgggacat gctcaatgag cgggcaaccg tggctcatga
     4621 ttggattccc cagcggttcg ttcctagcca agcgatcgat tggacaccaa tgtggtcact
     4681 aacggagcag acccataaat atgtacccat agcgatgtgt tactaccact acccgtcgcc
     4741 accaaatcac cacttctgcc gaaatgactc taatgggaac gcagcaggca ataccttgga
     4801 agaagcaatc ctacaagggt ttttggaact ggtggaacgc gacagtgtgg cgttgtggtg
     4861 gtacaaccgt ctgagccgtc cagcggtgga tctttgcagc tttaatgagc cgtattttgt
     4921 ccagttgcaa cagttctatc aggaaagcga tcgagatctg tgggttctgg atttgacagc
     4981 agatttaggt attccagcct ttgtgggagt atcgaatcgg aaaagtggaa gctcggaaag
     5041 actggttctt ggctttggag cgcacttaga cccaagcatt gccatcctcc gcgcccttac
     5101 agaagtgaac caaattggct gggaacttga taaagtgcca gacgagaacc tcaagggcga
     5161 ggcgacaaat tggttactga ctgcgacgct agcagatcat ccctatctgg ttcccgattc
     5221 tactcaagcc ctcaagactg cccaggatta ccccaaactt tggggggatg atatttacac
     5281 cgaggtgatg acctgtgtga agattgccca agaagctggt ctagagacct tggtggtaga
     5341 tcaaacacga ccggacattg gtttgaatgt ggtcaaggtg attgtgccag gaatgcggca
     5401 tttttggtcg cggtttgggt cggggcggct ctatgatgtg ccggtgaagc tggggtggcg
     5461 agatgaaccg ctcgctgagg ctcaaatgaa ccccacaccg atgccatttt aagtttttta
     5521 aagttttcgg gcgactcgct ttcgctacgt accggatgtc gcccgacgaa gcttcttctc
     5581 acgaagaagg ggactggccc accccaacgg gtcaataaac tcagcaacaa aatgaggatt
     5641 attccgatga ataagaagaa catcctgcct aacccaggca aacccgtgat ccgtggcatc
     5701 agtggtaaac tacccagcta cttggctgaa ttgtctgaag aagctctagg cgatgctggt
     5761 gcggatgcgt ctttcatctg ctcagtagat gcgtctttca tctgctcagt agatgcgtct
     5821 ttcatctgct cagtagatgc gtctttcatc tgctcagtag atggtgatgc ttaaagatct
     5881 ccgatttgct gttaattgca agcagattgc atcttaaggc tagtccacat ttgggaaaat
     5941 ggcagttcac tgtcctaact ataggaacta aagaacgcta gtcttgaggc taaagacgag
     6001 gctaaggatg cttggaaagc cgactttctc tcaagtctta ccttccttca tctgcctcgt
     6061 cttctttaat tcaacaagat gtattcaaac tcacaaccca gccattcaga ttttatcttt
     6121 ccgcaagtta tccccagaga acaccatgtc aaataattac attagctcta gtgttactac
     6181 tcatttgaac cagacacctg aagtcgaaaa agttcgtact ttttataaca atcactccat
     6241 caacttcagt cgaatggttc ctgatgcagt agcgagatcg cacaatcttg gtttagcagc
     6301 tcgcatgggg atacagcctg gaaactatat atttgatgcg ggttgtggag ctggaattcc
     6361 agctatccac atcgcccaat cgtttcctgg gacgcggatt gagggtctaa cgatttctga
     6421 agtggaagca gcagaagccc aaactcggat tgctcaaacc aacttggtcg atcgggtacg
     6481 agtgcaagta ggtgattttc attctccacc ttttccaaac gaagtctttg atgtggtatt
     6541 tttcaacgac agcatcaaat attctaatca attaccccaa gtccttgcgg aagtcaagcg
     6601 cgtccttcgt ccggggggaa ccctatacat tacgggtctt tttgttaaag agccgcccct
     6661 ctcgccacta gagcaacgag cgcttgatga aattaatcgg caagaattac aaggtagtta
     6721 tgttattcct cttaaaaact tggttgagtt tgtccagcag gcaggttttc aagccattga
     6781 atacgatgaa aaccaaactg ttaagatgcc tgcactcgct ttacgtctga ctgctgcaaa
     6841 cttaatgcac ccctatttac ctctactttt tgctgatatt aaagcagtaa aaccgacgat
     6901 agtcaaagct aaaagctcga tacgaccttc tggtttgacc tacaatcatg ctcaaaatcg
     6961 ccatcaggaa aatttgcgtt atatcgagca gcataagcaa atttttgata tggaaaattc
     7021 ctatttattg aatttgtttg agggacttgt cggaaatcaa cgaataggca aaggagggtc
     7081 tatttcctgt tgctgcaata tagataaaga tcgtctttat cccgctcgtt tttcatattt
     7141 atgtgaagtt catgtttccg atcctggaat taaattacta gatatcgaac attttctcta
     7201 tttctcccat cgtgtagaag aacgtccaga aattcagatc gatcgccgat tgcttaagca
     7261 gtttttagct acaggtctag acccgtccaa agtagatatg gtgatttttg ctattgatgt
     7321 tcgtccagaa ctcataaatt cgcggatcaa gttgtatttg accatcaaag attatccaga
     7381 aaaaatagca actgcaatat cgctttgcgg agaacagcgc gactgggaaa atttaataat
     7441 taacgggatg ttattggtag gatttgactt tttctttgat aatcgagctg ctgtagaagt
     7501 ttatcctacc ttctacgctg ccgatcttca gcgaccagat gtccaagcgt atttaacttc
     7561 gagactacct gcaaaagcac ttccactcct ccatgagagt agatgcgttc aaatcggtat
     7621 tagcaatgag aacaaaagtg atgttctcta tttcaataaa tttaaatctc ccaacaatgc
     7681 ggttgactat ctgaaaaatc aaatggttag ccaagtccat gctcagtatc gaaatcgagt
     7741 ctttcacgat ttatatcttg gtattcctga atcagagttt tatactgatt caatccaaag
     7801 agtaaaaatg tactatcaca tgaatgactt tgtaagcaga atgaaataaa gatgaatata
     7861 aaatagcctt gactctcaat gagtgcagct agttgggttt tgatacttac aaactgtttg
     7921 agaataaaat acgattatgc cgataacttc tgtattttca cacaattatc tacgagagca
     7981 gcgtctgaga tttatacgga ctcatcgaga tgcttttgat gtaccttata gctttttact
     8041 gcctctgtat gaagaagctg tttttaaaat agaaggctcg tgtggtgttg agcctagttg
     8101 taatgttgaa ggcgatcgct tatttgcagg agactttcaa gttgctaact atggacatac
     8161 ttggcctaga tcgctgattg atgcagcaag ttttttagac aaaattgaac gtagaataga
     8221 tgtaaaaatt aatcggaatt tgctcgagcg gttttcagca ctatatatag gctctagcaa
     8281 aatcgaaaat aacactatcg gcattgactt acgcccaaca cttcaggatt ctgtgattaa
     8341 gatatacatg cacatccatc ctgggcaatc gaacgaagat ttagtgatga cagcgctcgc
     8401 acttgatggt gctatatatt cggctgagtt gacacaagtt ttgctccgag atgttgttgt
     8461 gataggattt aatcttttct tggatggccg gagtaacata gaaatatggg cgggttctgc
     8521 aggacaaaaa cacaaccatc aaggaaattt gggaagagat ctcactgcct acataagaaa
     8581 atattactca cacaaagtaa attatatgtt taatgtttca gattttagcg cggtgagttt
     8641 ttctaagcaa aagatagacc cactcttcca ttttcacttt tttgatatca aagacatact
     8701 aaaatatttt gcatttaata gtttgggcga taaaatttat gatttttgtc aaagtcagga
     8761 ttgcattaca tatactggtg taagtgccag agcgcaagaa ttagaaagta atagattaaa
     8821 taattatagt tttttctata accaaagtga tacatgtcaa acagatttag atatactacg
     8881 cttacctatg atgttttaac tgttagagag gaaaactgag aaagtgatag cgtattaagc
     8941 aatcgttcaa cagtttcaaa aaaatcataa gttttgatat tacatcactc gaatttttcc
     9001 acatcaaata aaatttcatt tctgataatt tcaccaatcg ctactgccat cgactgactt
     9061 gcccaatcat cattatccat tatccgtcgc catgagctat catcccttcg ctgtttccct
     9121 caatcctttt gtgcaagtga caagcaccga agaccattac accctgcgct cggcagaatc
     9181 cgatccgacc gctttgctca ctatggctac aacgcccgga attggtatgg ctctcgatcg
     9241 tctcaaggca ggcaacacca cccccgactc actggtgcaa gggttagccg aactcgatgg
     9301 tcgggcagcc ggggaacagt tcgggcggat gctacaacaa ttggatgagc gaggttggtt
     9361 aagttacgct gtgttgccgc tggcagtggc aatcccgatg gtggactctg ccgagctgaa
     9421 tctgaccgaa ccctattgga cacaaacgca cctcactctc tcccgctttg cctatcagca
     9481 ttcccacgag ggcgcaatgg tgctggagtc ccctctctca aagtttcgga ttaaactcct
     9541 cgattggcga gcaagtgcga tcctggctca acttgcccag ccgcaatcct tagcaacact
     9601 aacgccaccg ccctacctgg ggccagaaac cgcctatcag ttcctcaatt tgttgtgggc
     9661 agcaggcttt ctgactgctg accctgaacc tccctcgctc cagctttggg aatttcataa
     9721 cttgctgttc cacagccgca gccgcttggg tcgccatgac tatccaccac aagatattaa
     9781 cattaagcag tggtctgatt ttccggtggt gaaatcgcct atgagccacc aaattgtccc
     9841 actgccgcgt ccgaacttag gagcgttaaa gtgtaatgat gctacactca cccaagcaat
     9901 tgaaacgcgg aaatcgattc gagaatacga tgaaacaaat ccaatcacca tagaacaact
     9961 cagcgaactg ctttatcgta cagcccgtgt caaagagatt cttcaaacgg aggagtggtt
    10021 tggtgaaaat tggcggcaaa tactcggttt tgatgcggcg attgaatatg gcgagttagg
    10081 tagtcggcca tatcctggcg gcggggcaat gtatgaactg gagatttatc cagtggtgcg
    10141 gcgttgtcga gtgttggatg caggcttgta tcactacgat tcactaaacc atcaattgga
    10201 aaggattgct gaatcggaag atgtttttgc tttgcaacca tatatttatt acaaacccca
    10261 accgcaagtc cttctcgtga ttacagcgcg gtttggacga ctgttccgca agtatcgctc
    10321 tgtggcctat tcgttagtgc tcaaacacgt cggagcgttg caacaaaatc tttatttagt
    10381 ggcaactaat atgggtctgg ctccctgcgc gttgggcagt ggggatagcg atggttttgg
    10441 gcaagccgca ggtctggact atgtggaaga gtcggcggtg ggtgagttca tgttgggctc
    10501 gctgccgaag cgtgatgtgc aagccagtgg ggttgagtca gttcaagaaa tcaatctctc
    10561 agatgagcct gcggagttag cagcctcgga gagtacggtt gaatcggata ggggtgaggc
    10621 ggaacctgag cctgctgagg ttgggtttcg ttcctcaact caacctacat ctgaactaga
    10681 tgttgggttt cgttcctcaa cccaagctac agaatcggtg gagacaacag aggtagcggt
    10741 ttcggtgagt gaggctgaat ctgtatccca gacgacgact caagtcatgg actcagaggc
    10801 ttccgagagt gaagtggaat cggatgttgt agtgaatgaa actgaatctg cggctatttc
    10861 agccagtggg gttgaaagtt ctgcaacccc actctcggca gcacttcacc cccacgacct
    10921 cgacgatcgc atccctggac tcgctcacct tcgcaatcaa actctcggcg acccccgtat
    10981 cactattgtg gttattgagg ggaaccccga ccactccctg tcctgtttcc agagcgctga
    11041 aatttctaaa gtctttccct actgggagga cactccagag ccgatttcct cagaagagta
    11101 cgcgactttc cgagcaattg aggatagcga tcgcacgaaa aaggagaaag aagaatcact
    11161 caaagcggct tttccagaaa cgctcatcca acgtcttaaa gttgatgacc atacttgcca
    11221 cgtcaccagc atcattgtgg gtcaagagaa cactccttct gcgggtattg ctccccactg
    11281 tcgcctgatt aatctgtcgc ttaccagtgg catagcagaa gaatatatct ctcccttgaa
    11341 cctagctcgt gcctttgagc aagctctcga tttgggggca aatatcatcc actgcgggat
    11401 ctgccatcct accaaaactg gcgaagggga agagctattc gctaaagccg tacagaagtg
    11461 cctagataac aacattctga ttgttgggcc tgcgggtaac ggtttgggtg aatactggtg
    11521 tctgcccggt gtattgccag gaaccctgcc tgtaggagca atggatctag gcagtccacc
    11581 cgcccactac agtaactggg gaggcaatta taccgaagag gggattatgg ctccgggtga
    11641 cgacatttgg ggagcgcaac tggcaaccga gaaacccaga ctcagaacgg gaaccagtgg
    11701 agccgcccct atcgtcaccg gaatcgccgc gctgctgatg agtttgcaac tgcaagaggg
    11761 caaacccgtc gatgctgaag ccgtccgaac ggcactgctc aaaaccgcaa ttccctgcga
    11821 cccaaaggtt gtagaagaac cggaacgctg tctgcgcggc tttgtcaata tcggcggcgc
    11881 aatgaagctg ctgtttgggc gaccctcctt caccatttcc ttcgcgggcg atcgagtgac
    11941 ccgcacggaa cggggaggct atgccatggc taccgctacg gctgccccgg caagcatcgc
    12001 agcggctatg acagacactg ccgtcagccc agccctgcaa cccgccaccc ctgccacact
    12061 aaccgaggaa cccgtcacag ccgccgcgac tcccgcagac acaggcactg tcgaagccag
    12121 caccgccttc tctggcaacg tttacgccct agggacgatc ggctacgact ttggggatga
    12181 aacccgccgc gacactttcc aacaacagat ggctcccgcc gaagtcaacg gcattatggt
    12241 tccgccagac ccccacgatc ctcgtcagat ggtcgaacac ctcgatcgcc atccagatga
    12301 aagccgttcc ctgatctgga ccttgagcct cgatggagac acgatatatg ccctagagtc
    12361 aaaagatggg gatgtctacg agatgtttgt gctgatgttg gcggggcaac tcgagccaga
    12421 agcgagcgat gaatttgtag aacggatcag catacccgcc cgacgcacca agcgcacggt
    12481 agaacttttc tccggtgagg ttgtgcctgt agtgacggta cctaacgtgc ggggaatgta
    12541 cggctggaaa gtcaataccc tggtgaactc ggcgatggcg gcaatctccc gtcaggtgga
    12601 cgaagcccaa gaacccctcg tgcgacaggc tctgacagcc ttcctcaacc gagtctacaa
    12661 cgatcttcgc aatgtggggc agacttcgcg cgatcgcgcc ctcaacttcg ccgccaccaa
    12721 catcttccaa gccgctgtga cctttgctcg ggcgatcgtc gatcgtcgtc aactcgacac
    12781 cattaacgtg gaaaaaagcc cgttctgtcg catcaacagt gactgttggg atgtcaagct
    12841 tgtcttctac gacccagaca atactcgtcg ggggcgcaag gtattccgct ttacccttga
    12901 tgtggtgctt ctgatgccgg tgactttggg tcaggtgaga agctggtcac tccctagtaa
    12961 agagagttga
//
