LOCUS       BGC0000473             13853 bp    DNA     linear   BCT 14-MAR-2008
DEFINITION  Microcystis aeruginosa NIES-298 microcyclamide biosynthetic gene
            cluster, strain NIES-298.
ACCESSION   BGC0000473
VERSION     BGC0000473
KEYWORDS    .
SOURCE      Microcystis aeruginosa NIES-298
  ORGANISM  Microcystis aeruginosa NIES-298
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Chroococcales; Microcystaceae; Microcystis.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:42:27
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13853
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /clone="epifosNIES298-mca5"
                     /db_xref="taxon:449468"
                     /mol_type="genomic DNA"
                     /organism="Microcystis aeruginosa NIES-298"
                     /strain="NIES-298"
     subregion       1..13853
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13853
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            868..2841
                     /gene="mcaA"
     CDS             868..2841
                     /codon_start=1
                     /db_xref="GOA:A8Y990"
                     /db_xref="InterPro:IPR000209"
                     /db_xref="InterPro:IPR015500"
                     /db_xref="UniProtKB/TrEMBL:A8Y990"
                     /gene="mcaA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 158.7; E-value: 4.7e-48)"
                     /gene_kind="biosynthetic"
                     /product="subtilisin-like protein"
                     /protein_id="CAO82081.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 6.9e-34, bitscore:
                     106.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 4.3e-236,
                     bitscore: 773.0, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDRLHTISQGDSSICVAVLDGPVDQAHPCFQGANLTYLPTLVQE
                     AAKVDGSMSMHGTHVASILFGQPGSPVQGIVPQCKGIIIPIFADDRRRTSQLDLARGIE
                     QAVHAGAHIINLSGGQLTDFGESDGWLKNAVRLCRENNVLLVAAAGNNGCECLHVPAAL
                     PSVLAVGAMDAQGKPLDFSNWGEAYQTQGILAPGEDILGAKAGGGTHRLSGTSLATPIL
                     SGVAGVLLSLQREQGDSPDPQKIRQILLQSALPCDLDLPEESRRCLAGKLNVSGAITLL
                     KGEKMAEEFSSVAASEVTAAGCGCGGTLSATPQEQTAPSLTAVASSPSAGAVPSETPNL
                     NNLIQPFPTAVTQQPTMPNITANSVTASEAPSQIADLGQIVYVLGTLGYDFGTEARRDS
                     FKQLMPPFDLDGGIMVPANPYDARQMVDYLGENISESRSLIWTLNIELTPVYAIAPTGP
                     FASDVYRALQELLSGQIQPESDAEYMERVSIPGVLTGKKVKLYSGQIVPVIEPLGTRGI
                     YGWKVNSLVNAAMEAVQAEDGTADEDRIRKTLDGFLNRIYYDLRNLGTTSQDRALNFAV
                     TNAFQAAQTFSQAVAVGMELDSVTVEKSPFCRIDSDCWDVKLKFFDPENSRRAKKIFRF
                     TIDVSDLIPVTLGEVRSWSSPY"
     gene            2944..3195
                     /gene="mcaB"
     CDS             2944..3195
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A8Y991"
                     /gene="mcaB"
                     /product="hypothetical protein"
                     /protein_id="CAO82082.1"
                     /transl_table=11
                     /translation="MRLPILSPPVKRPHFIQPALCVDLENGRPEDLVHIRMDLLHAANY
                     NDPAAFANRSFNQVMHSSASQSSASQGWGGFGSMAGFY"
     gene            3246..3488
                     /gene="mcaC"
     CDS             3246..3488
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A8Y992"
                     /gene="mcaC"
                     /product="hypothetical protein"
                     /protein_id="CAO82083.1"
                     /transl_table=11
                     /translation="MSKNKTTTTSSEVPSNPEPTPPEPSKSEPTTITTPEPKKSYVPTS
                     QTGLQDYSYWCQYVKDQAKKSDKDPKSFRRGRIWA"
     gene            3550..5880
                     /gene="mcaD"
     CDS             3550..5880
                     /codon_start=1
                     /db_xref="GOA:A8Y998"
                     /db_xref="InterPro:IPR003776"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR022291"
                     /db_xref="UniProtKB/TrEMBL:A8Y998"
                     /gene="mcaD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cyanobactin_D_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NHLP_CD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Goadsporin_CD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) YcaO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03604"
                     /gene_kind="biosynthetic-additional"
                     /product="adenylation/heterocyclization protein"
                     /protein_id="CAO82084.1"
                     /sec_met_domain="Cyanobactin_D_RRE (E-value: 3.2e-44,
                     bitscore: 138.3, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="NHLP_CD_RRE (E-value: 1.3e-22, bitscore:
                     69.1, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="Goadsporin_CD_RRE (E-value: 1.4e-20,
                     bitscore: 62.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 2e-99, bitscore: 322.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 1.6e-85, bitscore:
                     276.4, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQSTPLLQIQPHFHVEVIEPKQVYLLGEQANYALTGQLYCQILPL
                     LDGQHSREQIVEKLDGEVPSEYIDYVLDRLAEKGYLTEAAPELSSEVAAFWSELGIAPP
                     VAAEALRQSVTLTPVGNISEVTVAALTTALRDIGISVQTPTEAGSPTALNVVLTDDYLQ
                     PELAKINKQALESQQTWLLVKPVGSVLWLGPVFVPGKTGCWDCLAHRLRGNREVEASVL
                     QQKQAQQQRNGQSGSVIGCLPTARATLPSTLQTGLQFAATEIAKWIVKHHVKATAPGTV
                     FFPTLDGKIITFNHTVIDLKSHVLVRRSQCPSCGDRQILHRQGFEPVKLVSRRKHFTHD
                     GGHRAFTPEQTVQKYQHLVSPITGVVTELVRLTDPANPLVHTYKAGHAFGSATTLRGLR
                     NTLKYKSSGKGKTDIQSRASGLCEAIERYSGIFQGDEPRKRATLAELGDLALHPESLLY
                     FSNTQYANREELNAQGSAAAYRWIPNRFDVSQAIDWTPVWSLTEQKHKYVPTAFCYYGY
                     PLPEEQRFCKADSNGNAAGNTLEEAILQGFLELVERDSIAMWWYNRIRRPAVDLSTFDE
                     PYFVDLQQFYQQQNRELWVLDVTADLGIPAFAGFSRRTVGTSERISIGFGAHLDPTIAI
                     LRALTEVSQVGLELDKIPDDKLDGESKDWMLNVTVENHPWLAPDPSVPMKTASDYPKRW
                     SDDIHTDVMNCVKTAQTAGLEVMVLDQTRPDIGLNVVKVIIPGMRTFWTRFGQGRLYDI
                     PVKLGWLDAPLAEEELNQTNIPF"
     gene            5997..6221
                     /gene="mcaE"
     CDS             5997..6221
                     /codon_start=1
                     /db_xref="InterPro:IPR022264"
                     /db_xref="UniProtKB/TrEMBL:A8Y993"
                     /gene="mcaE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_precursor"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: TIGR03678"
                     /gene_kind="biosynthetic"
                     /product="microcyclamide precursor protein"
                     /protein_id="CAO82085.1"
                     /sec_met_domain="TIGR03678 (E-value: 3.8e-27, bitscore:
                     83.7, seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_precursor (E-value: 1.0,
                     bitscore: 1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRITPMDKKNILPQQGKPVFRTTTGKLPSYLAELSEEALGGNGLE
                     ASHCATICAFDGAEASHCATICAFDGDEA"
     gene            6516..7481
                     /gene="mcaF"
     CDS             6516..7481
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A8Y994"
                     /gene="mcaF"
                     /product="hypothetical protein"
                     /protein_id="CAO82086.1"
                     /transl_table=11
                     /translation="MTLTSMLTNNHLKARRLQFLRGYQEAFDVEPNFMLSLFEEAVLGI
                     EETCGVESKCNVEKDQLFAIDFQVCNDQGRTWPMSLTHAVKFMDKIESTVGVRLNRNLL
                     EQFATLHLDSHKIQDNTVGIDLRPRNEDSCIKVYLHLGSEEEPEELVRTALELDGGSYS
                     PELLQVLLKSTIVIGFNLFLNGYSDLELWATCPGEQYEVPNSDRGKYLKQYVQNNFSQK
                     INDLLRESTFLVVSFSNQKEPALIFHYEDIKEIPKNFLLNSLGDRIYSFCQGQDCMTYA
                     GVAVTERELEKDRLENFSILYNQRDECKPLLHIKKREDFS"
     CDS             7534..8337
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A8Y995"
                     /note="ORF1"
                     /product="hypothetical protein"
                     /protein_id="CAO82087.1"
                     /transl_table=11
                     /translation="MGIEFNHLYVTLDAETLDSIAKSEFISQEFCTISRDTVKTDTESW
                     TGIYLRGKHSYLELFPPGGAEGLREGFSGIGFNSQQAGEMDIVQEKLRPLLGAKEILSD
                     LQVRQTEAGKVPWFYYLSINPVEREAFASWLMEFHQDYLDYKNIEMTSAGCFNRAAYLK
                     TLETSETSLFDDISEVHLELTLPEQEELALLLQAFGYDSSSAGDITTYHSHNFMIKVCQ
                     KVARRYRICKVVCTLKEQLQQEKTFTFGKNAQLNLGRSLAIWEFG"
     CDS             8426..8773
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A8Y996"
                     /note="ORF2"
                     /product="hypothetical protein"
                     /protein_id="CAO82088.1"
                     /transl_table=11
                     /translation="MINNCPFAISLNPDVQIIDTKDGYTLHGAESEPNTWLTLAITSSK
                     LLFSFPTLLLVLPAELKIVESVFEYLLKCSVPRLNHLLTNGLIKCQELYLNQQNSLTTV
                     KGYHFHKCWPG"
     gene            9166..13221
                     /gene="mcaG"
     CDS             9166..13221
                     /codon_start=1
                     /db_xref="GOA:A8Y997"
                     /db_xref="InterPro:IPR000209"
                     /db_xref="InterPro:IPR000415"
                     /db_xref="InterPro:IPR015500"
                     /db_xref="InterPro:IPR020051"
                     /db_xref="InterPro:IPR022398"
                     /db_xref="UniProtKB/TrEMBL:A8Y997"
                     /gene="mcaG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 86; E-value: 5.3e-26)"
                     /gene_kind="biosynthetic"
                     /product="thiazoline oxidase/subtilisin-like protease"
                     /protein_id="CAO82089.1"
                     /sec_met_domain="PF00881 (E-value: 4e-15, bitscore: 45.5,
                     seeds: 78, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S8 (E-value: 2.7e-22, bitscore:
                     68.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 1e-181,
                     bitscore: 593.9, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPPQFLTSNKIPFLIISPIAAVIDSLPNHRSPLSINMNNCPFAI
                     SLNPAVQVTSTKDGYTLHGAESEPNAWLTVATTPGMGMALDSLKAGNTTPELLVQGLAE
                     LEGQAAGEQFGQMLQQLDERGWLSYAVLPLAVAIPMVDSAELNLTEPYWTQTRLSLSRF
                     AYQHPYEGAMVLESPLSKFRVKLLDWRASAILAQLAQPQTLGSLTPPPYLGAETAYQFL
                     NLLWATGFLTADPEPPSLRLWEFHNLLFHSRSRLGRHDYPVIDYDLEQWSDFPAVKPPM
                     SDKIVSLPRPNLQALMCNDATLTEAIERRRSIRGEDDDNNPISIEQLGELLYRTARVKK
                     CVSPQEMFGKYWLKEQPILEEAGVDYGELTRRPYPGGGGMYELEIYVIVRLCQGLSQGV
                     YHYDPLNHHLEMIFEFESDTDILTTSGYGIWNAIAQAPQVILVITARFGRLFRKYRSVA
                     YALVLKHVGILKQNFYLVATNMGLAPTAAGVGDSDAFAQITGLDYLEESVVGEFMLGSL
                     PSSNVEASGLESMEVDGSEEPAEATVSASDTGLAGMELEPDSGEPEPGQAEILGNQLES
                     VGSVAEIASAEIGESPENTSEQVEETEAEAVELTASDAIAQTPDSSSVAEGEKSAGVWA
                     SEVESSATQLSIALHPHDLDDRIPGLAELHNQTLGDPRITIVILDGNPDHTLSCFAGAN
                     VSKVFPYWHEPADPISPEDYATFQAIRDQGLKGKAKQEALESAIPDTINRVELNDHACH
                     VTSTIVGQEHSPVFGIAPNCRVINMPHDAVVTSDNGIALSGYNEVLSPLNLARAFELAI
                     ELGANVIHCAFCRPTQTGEGEEILVKAIKKCIDNNILIVSPTGNNLGECWCMPAVLPGT
                     LAVGAAKVDGTPCHFSNWGGNNGEEGILAPGEDILGAQPCTEKPVRLTGTSMAAPVMTG
                     IAALLMSLQVQQGKPVDAEAVRTALLNTAIPCDPNVVEEPERCLRGFVNIPGAMKVLFG
                     QPSVTISFAGDQVTRTEWAGYGTAIAGDTSSIPAGITAIAVSSVGDSTMTMQSATPMAA
                     EVAVTAAADPATVEASTAFSGHVYALGTLSYDFGDETCVDTFKERMAPVEMDSILVPPD
                     PYDPRQMVEHLDRNPDESRSLIWTLSLDGDTIYVLEPTGAFSDQIYEMFVLMLVGQLEQ
                     KSSDEFVERISIPARQTNRTVELFSGQVVPVVKVHDIRGMYGWKVNTLVNAAMVAISRQ
                     VDEAQAPLIQQALTSFLNRVYNDLRNVGQTSRDRALNFAATNIFQAASVFAKAIAERRQ
                     LDTIEVEKSPFCRRNSDCWDIKLEFSDPERSSRGRKVFLFTLDVVLQMPVTLGKIKSWS
                     MPSRSEQT"
ORIGIN
        1 gagagattgg ccagggttct tttttgactt gatctcctct cggtaggact cccgaagaag
       61 aacggcgggg gggggaatta cggttaagaa ctttttctat atacatggct atcattctag
      121 caaagggaga gaattcttgt ccattaagaa atgttacaaa ttacatgggt acattaagaa
      181 aagttaaggg gtgtatccat tgatggataa gggttttcgg ctattgtagt atatatgtat
      241 ggggtaagtt caggtatcag gattgtacaa cgatttttcg cgggatattg aaaagcatag
      301 acactattaa taataattct caatagttac ctcggtcata aggatcaaag ccttgctgga
      361 caaggcatcg acgcacctca acgaaaactc tagattttca gccattgtgt ctaacccgcg
      421 aaaaatgttg agaacccccc cattccatcg gcatgaaaca ataacccaca ttccgcaccc
      481 taaagtgtca cattgaaaat tttatctaaa tcgtcaaaac gattgccggc tctgaattac
      541 aggctaattt gtttgcccag cagatgcaca attagcccta aaaatctgat agctctgata
      601 ccaaattatt gagataaact ctcattaagc cagcgattgc tggctaaagt gtgacacttc
      661 ataattatta ataatcgcta aaagttttac tatacaaggg ctagagcaac ttttgatttc
      721 gctgaaacaa tccactacaa ctaaactatg gtatgataaa aattatggtt agcttataat
      781 gtcagaaccc ccttaatcac aatttttgag gtaccattat tttgtcaaga atctaggcta
      841 cataagtaaa aatgataaca cctttaaatg ctagatagat tacatactat ttcccaagga
      901 gatagcagta tttgtgtggc tgttttggat gggcctgttg atcaagctca cccttgcttc
      961 caaggcgcaa accttaccta tctgcccaca ttggtgcaag aagccgctaa agttgatggc
     1021 agtatgtcca tgcacggaac tcacgttgcc agcattctct ttgggcaacc cggtagcccc
     1081 gttcagggaa tcgtacctca atgcaaaggc attatcatac cgatttttgc cgatgaccgt
     1141 cgccgaactt cccagctaga tttagctcgg ggaatcgagc aagccgttca tgccggagcc
     1201 cacatcatca acctcagtgg gggtcaactc actgactttg gtgagtccga tggctggtta
     1261 aaaaatgctg ttcgtttgtg ccgagagaat aacgtcttgt tagtggcagc agcaggcaat
     1321 aatggttgcg agtgtttgca cgttccggca gctttaccgt cagtgctggc cgtcggggca
     1381 atggatgccc aggggaaacc cctcgacttt agcaactggg gggaagccta ccaaactcaa
     1441 ggcatcctgg ctcctgggga agatattctg ggagccaaag ctgggggggg aacccaccga
     1501 ttaagtggaa ccagtttggc cactcctatt ctttctggtg tcgctggggt attactcagc
     1561 cttcaacggg aacagggaga ttcccccgac ccccaaaaaa ttcgtcaaat tttgttgcag
     1621 agtgctttac cctgtgacct tgacttgcca gaagaaagcc ggcggtgtct ggcgggcaaa
     1681 cttaatgtct ctggagcaat cacgttacta aaaggagaaa aaatggctga ggaattttcg
     1741 tctgtggcgg catcagaagt aacagcagca ggttgtggtt gtggaggcac gttgagtgct
     1801 accccccaag agcaaacggc tcctagctta actgctgtgg cttccagtcc atcggctggg
     1861 gctgttccca gcgaaactcc caacttaaac aacttaattc aaccattccc tactgctgta
     1921 actcaacaac caactatgcc taacattact gctaattccg tcactgccag cgaagcccct
     1981 agtcaaattg ccgatctcgg tcaaattgtc tacgttttag gaactctagg ttatgacttc
     2041 gggactgaag cccgtcggga ctcgtttaaa caactcatgc ctccctttga tttagatgga
     2101 ggaatcatgg ttcccgccaa cccctacgac gcccgtcaga tggtggacta tctaggggaa
     2161 aacatctcgg aatcgcgatc gctgatttgg acattaaaca ttgaactgac tccggtctat
     2221 gcgatcgcgc ccacgggtcc gtttgcgtcc gatgtttatc gcgctctcca ggaactgcta
     2281 tccggtcaaa tccagcctga gagcgatgct gaatacatgg agcgggtcag catccctggc
     2341 gttttaactg ggaaaaaggt caagctatac tccggtcaaa tcgtcccggt gattgaaccc
     2401 ctgggaaccc gaggaattta cggctggaag gtcaacagct tggtgaatgc ggcgatggaa
     2461 gcagtgcaag cggaagacgg aactgccgac gaagaccgga ttcgcaaaac cttggatggc
     2521 ttcctcaacc gcatctacta cgacctccgc aacttgggaa ccacttccca agaccgcgcc
     2581 cttaactttg ccgtcaccaa cgccttccaa gccgcccaaa ctttcagcca agcggtagcc
     2641 gttggcatgg aactcgatag cgttacggta gagaaaagcc ccttctgccg gatcgatagc
     2701 gactgctggg atgtgaagct gaagttcttc gacccggaaa acagccgtcg ggctaagaag
     2761 attttccgct ttaccattga tgtaagtgac ctaattcctg tcaccctcgg cgaagttcgt
     2821 tcttggtctt ctccttacta aggagttgta ctggcttagt gagagtgaag tttactattg
     2881 cactctcact gggacatccc aatgtgagaa atctattaat cggattacgg agaatcttaa
     2941 accatgcgac tacctatctt gtctcctcct gtcaaacgtc cccatttcat ccagccagca
     3001 ctgtgtgtgg atctagaaaa tggtcgtccc gaagatttgg tgcatattcg gatggatctg
     3061 ctccatgccg ctaactataa cgatcctgca gcctttgcca accgtagttt caaccaagtg
     3121 atgcactcta gtgccagcca aagttcagcc agccagggct ggggcggctt tggcagcatg
     3181 gcaggattct attaatgggc tgctaatcac tcgccctcag ccaaccctaa acatccaagg
     3241 acactatgag caagaacaaa accacaacaa catcctccga agtcccctct aaccctgaac
     3301 cgaccccgcc tgaaccgagc aagtctgaac cgacgacgat cacaacgcct gaacccaaaa
     3361 agtcttacgt tcccacttcc cagaccgggc tgcaagatta ttcctactgg tgccaatacg
     3421 tcaaggatca agccaagaaa tcagacaaag accctaaatc cttccggcgc ggtcgcattt
     3481 gggcttaacc cctagctgct gctgctgctg gtcttgttat cgtccactta atcactgact
     3541 gttcgttcta tgcaatccac cccactgctc caaattcaac cccactttca tgtcgaggtg
     3601 atcgagccaa agcaagtcta tctccttggc gaacaggcta actatgccct gactggacag
     3661 ctttactgcc agattttgcc cctcttggat gggcaacaca gccgtgagca gattgtcgag
     3721 aaactggatg gggaagtccc atcagaatac atcgactatg tactcgatcg cctcgctgag
     3781 aaaggttatc tgactgaggc agccccggaa ctgtcctcgg aagtggcggc attttggagc
     3841 gagttaggga ttgcacctcc agttgcggcg gaagctttgc ggcaatctgt aaccctaacc
     3901 cctgtgggca acatcagtga agtgacggtt gcagccctga caacggctct gcgggacatt
     3961 ggtatttccg tgcagacccc aactgaagca ggttctccaa cggcgctcaa cgtcgtctta
     4021 accgatgact atttgcagcc cgaactcgcc aagattaata aacaggcact agagagtcaa
     4081 caaacttggc tactggtcaa acctgtaggc agtgtgctgt ggctggggcc tgtgtttgtt
     4141 ccgggcaaga cggggtgttg ggactgtcta gctcatcggc tacggggcaa ccgggaagtg
     4201 gaggcatcgg tgttgcagca aaaacaagcc cagcagcaac gcaatggtca gtccggttct
     4261 gtaatcggtt gcctgcctac cgcacgggct accctgcctt ctaccctgca aacggggcta
     4321 cagttcgcgg caactgaaat tgctaagtgg attgtcaagc accacgtcaa ggccactgca
     4381 ccgggaacag tgttctttcc cactctggac ggcaagatta tcacctttaa tcacacagtt
     4441 atcgacttga aaagtcatgt tctcgtccga cggtcccaat gtcctagctg tggagatcgc
     4501 cagattttgc atcggcaggg ctttgaaccc gtaaaactgg tatcgcgtcg taaacacttt
     4561 acccatgatg gcggtcatcg tgcgtttact ccagagcaga ccgtacaaaa atatcaacat
     4621 ctggtcagcc ccataactgg ggtagtgaca gaactggtca gactcaccga tccggctaat
     4681 cctttggtgc atacctacaa ggcaggtcat gcctttggca gtgctacaac tctgcggggt
     4741 ttacggaata ctctgaaata caagagttct ggtaagggta aaactgatat tcaatcccgt
     4801 gccagtggtt tatgtgaagc catagaacgc tattcgggca tttttcaggg agatgaaccc
     4861 cggaaacggg caaccctagc ggaactggga gacttagcct tacatcctga gtcattgctg
     4921 tactttagca acacccaata tgccaaccgg gaagagttaa atgctcaggg ttcagccgcc
     4981 gcttatcgct ggattcctaa ccgttttgat gttagtcaag cgatcgactg gacacctgta
     5041 tggtcattga cggagcagaa gcataaatat gtccctactg ccttttgcta ctacggttat
     5101 cctttgcccg aagaacagcg cttctgtaaa gcagattcta acggtaatgc tgcgggtaat
     5161 actttagaag aggcgatcct acaaggattt ctggaactgg tagagcgcga cagtatcgct
     5221 atgtggtggt acaaccggat acgccgtcct gccgtagatc tgagtacctt tgatgaaccc
     5281 tattttgttg atttacagca attctatcag caacaaaatc gggagttatg ggtgttggat
     5341 gtaactgctg atctagggat tccagccttt gcagggtttt cacggcgcac ggtgggtact
     5401 tcagaacgga tctccatcgg cttcggagcg cacctcgatc cgactattgc gattctccgc
     5461 gccctaactg aagtcagtca agtgggatta gagttagaca aaataccgga cgataagctt
     5521 gatggcgagt ccaaggactg gatgctgaat gtaacagtag aaaatcatcc ttggttagcg
     5581 cccgatccct ctgtgcccat gaaaactgcc agtgattacc cgaaacgttg gagtgatgat
     5641 attcacaccg atgtgatgaa ttgtgtcaaa actgcccaaa cagcaggact agaggtgatg
     5701 gtcttagacc aaacgcgacc ggacatcggt ttgaatgttg tcaaggtgat tatcccagga
     5761 atgcgaactt tttggacgcg gtttggtcag ggacggctct atgacatacc cgtaaagctg
     5821 ggatggctcg atgcgccact agctgaagag gaattgaacc agaccaacat ccccttttaa
     5881 gttttttaaa gtttaggggc gacagatttc tctacatatc cttgccccac caagcttctt
     5941 cccaaagaag aaggggactg acccacccct aacgggttaa taaagcaaac aacaacatga
     6001 ggattactcc aatggataag aaaaacattc tgccccaaca aggcaagcct gtgtttcgta
     6061 ccaccaccgg taaactcccc agctacttag ctgaactctc tgaggaagct ctcggtggta
     6121 atggtcttga agcctctcac tgtgccacta tctgcgcttt cgatggtgca gaagcctctc
     6181 actgtgccac tatctgcgct ttcgatggtg acgaagcgta aggatagcct ataccaaatc
     6241 tgtgagaata gggtctatct gactgattag cctatctcaa ccggatttag tgtattcgtt
     6301 tatttgagaa aacgaggtta aagatgttga gtcgcactcc aaactttttc ctagattggg
     6361 taagcttttc tcatctacct cgttttcttt taattgggtt tttaagttct cattgtaccc
     6421 aattggacac gacttacgca gccacagcac ctgtaggggc aatcccctta ccattggtat
     6481 aaagggacta aaacccaaca acaaacctac tggaaatgac cctaacttct atgctgacaa
     6541 ataatcatct caaagcccgt cgtcttcagt ttttgcgggg ctatcaagag gcttttgatg
     6601 tggaaccaaa ctttatgctt tcgctgtttg aagaagccgt ccttggcata gaagaaactt
     6661 gtggtgttga atctaaatgt aatgttgaaa aagatcaatt atttgccatt gattttcagg
     6721 tttgtaatga tcaaggacgt acttggccaa tgtctttgac ccatgcggtc aagtttatgg
     6781 ataaaattga aagtacagta ggtgttcgac tcaatcgtaa tttgctggag cagtttgcaa
     6841 cattacacct ggactcccat aaaatacagg acaacacagt cggaattgat ctaagaccta
     6901 gaaatgagga ttcttgtata aaagtatatc tgcatcttgg ctcagaagaa gagcctgaag
     6961 aattagtaag aacagcactc gaacttgatg ggggtagcta ttcacctgaa ctcctacagg
     7021 tattactcaa aagcactatt gtaattgggt ttaatctttt cttgaatggc tacagcgatc
     7081 ttgagttatg ggcgacttgt ccgggagagc aatatgaagt ccccaattca gaccgaggca
     7141 aatatttgaa acagtatgta cagaataatt tttcccagaa gatcaatgat ttgttgagag
     7201 aatccacttt tttggtagtt tctttttcca accaaaaaga gcctgcgcta atctttcatt
     7261 atgaggatat taaagagata cccaagaact tcttattgaa tagtttaggg gatagaatct
     7321 acagcttttg tcagggtcaa gattgtatga cttatgctgg agtagctgtc acagagcggg
     7381 aattggaaaa agacaggtta gaaaacttta gtattcttta taatcaacgg gatgaatgta
     7441 aacctttgtt gcatattaaa aaacgagaag attttagtta gtcttctccc cctctccccg
     7501 tctctacttt agttatgctt actgcctaag aatatgggaa tagaatttaa ccatttatat
     7561 gttaccctcg acgcagaaac cctagattca attgccaaat cagagtttat tagccaagaa
     7621 ttctgcacaa tatccagaga tactgtaaaa actgatacag aaagttggac gggaatttat
     7681 ctcagaggaa aacattccta tctagagctt tttcctcccg gcggtgctga agggttgagg
     7741 gaaggttttt ctggtattgg ttttaatagt caacaagcag gagagatgga tattgtacag
     7801 gaaaagctta gacccttatt aggagctaaa gaaatacttt ctgatttgca agttcgtcaa
     7861 accgaagctg gaaaagttcc ctggttttat tatttgtcca tcaacccagt agagagagaa
     7921 gcttttgctt catggttgat ggaatttcat caggattatt tagattataa aaacatcgaa
     7981 atgaccagtg cgggttgctt taaccgtgcg gcttatctga aaacccttga gacttccgaa
     8041 acctcattat ttgatgacat atcagaagtt catcttgaat tgactttacc ggaacaggag
     8101 gagttggcat tacttcttca agcttttggc tatgactcat ctagcgcagg agatattact
     8161 acctatcatt cccataattt tatgatcaaa gtatgccaga aagttgctcg ccgttatcga
     8221 atttgcaagg ttgtttgcac tctcaaagag caattacagc aggaaaaaac ttttactttt
     8281 ggtaaaaatg cacagttaaa tctgggtaga tccttagcaa tttgggagtt tggatgactc
     8341 aagcaatcca taaaaattaa gacaaatttc accaattgct gttttcattg ctccacctat
     8401 ttaataccgt ttcctattat taatcatgat caacaattgt ccttttgcta tttcccttaa
     8461 ccctgatgtc caaatcatcg acactaaaga tggctatacc ctgcacggag cagaatctga
     8521 gccgaatact tggctaacct tagcaataac gtcttcaaaa ctgctcttct ctttccctac
     8581 tcttcttcta gtgcttcctg ctgaattgaa aatcgttgaa tccgtctttg aatatctgct
     8641 aaaatgctcc gtacctcgtt taaatcactt gttaacaaat ggtctgataa aatgtcaaga
     8701 actatatcta aatcaacaga atagtttaac aacagtaaaa ggatatcatt ttcataaatg
     8761 ctggcccggc taaattgtcc ttgacgatag agagatggca aggcataaca ctttaaaatt
     8821 aggagtccta ccactgtgac acatcgaact tttcggtttc caaagatctg ctctgctaca
     8881 aactcttgcg atatttttcg gaagagctta ttttgggtta acaatacgtc aacttgtaac
     8941 ccatcaaatc ttccccgaag aaaattttta tcttcactct ggataccaat ctctggtaaa
     9001 gtgtcaagat cggcacgagc caggatcagg tcaatatctt gagtattgcg accctccaca
     9061 tagctgagta gagctacccc gccaacgaga acataacgaa tttctcgttc agcaagcgcg
     9121 tcaaataatc gttcaacagt ctgaaaaaaa tcagaacttt ctgacattac accaccccaa
     9181 ttcttaacat caaataagat tccatttctg ataatttcac cgatcgctgc tgtcattgat
     9241 tcactgccca atcatcgttc tccattatcc atcaatatga acaattgccc ttttgctatt
     9301 tcccttaacc ctgctgtcca agtcaccagc actaaagacg gttataccct gcacggagca
     9361 gaatctgagc cgaatgcttg gctaaccgtt gccacaacgc ccggaatggg tatggctctt
     9421 gatagtctca aggcaggcaa caccacccct gaattactcg tgcaagggtt agccgaactc
     9481 gagggtcagg cagccgggga acagtttggg cagatgctac aacaattgga tgagcgaggt
     9541 tggttgagtt atgccgtgtt acccctagca gtggcgatcc cgatggtgga ttccgccgag
     9601 ttgaatctca ccgaacccta ctggacacaa acccgtctca gcctctcccg ttttgcctat
     9661 cagcatccct acgaaggcgc gatggtattg gagtcccctc tgtcgaagtt tcgggtcaag
     9721 ctcctcgact ggcgagccag tgcgatctta gctcaactgg cccagcccca aactttagga
     9781 tcactgacac caccgccgta cttgggtgca gaaaccgcct atcagtttct caacctgctg
     9841 tgggcaacgg ggtttctgac ggctgacccc gaacccccct cgcttcggct ttgggagttt
     9901 cataacttgc tgttccacag tcgcagccgc ttgggtcgcc atgactaccc tgttatagac
     9961 tatgatctgg aacaatggtc agatttccca gcagtaaagc cacccatgag cgacaaaatt
    10021 gtgtcgctgc cacgtccgaa cttgcaagca ttgatgtgta atgatgccac ccttacggaa
    10081 gcgattgaaa ggcgaagatc gataagagga gaagatgacg acaataatcc gatctcgatc
    10141 gagcaactgg gggaacttct ttatcgcact gcccgtgtta agaaatgtgt ttctccacag
    10201 gagatgtttg gcaaatattg gctgaaagaa cagccaatat tggaagaagc aggtgtagat
    10261 tatggcgagt tgactcgtcg cccttatcca ggtggtgggg gaatgtatga attggaaatc
    10321 tatgtgatag taaggctgtg tcaagggtta agtcaagggg tgtatcacta cgatccacta
    10381 aatcaccacc tggagatgat ttttgaattt gaatcagata ctgatatctt gactacatca
    10441 ggttatggga tatggaatgc tattgctcag gctccacaag ttatcttagt gattacggca
    10501 cgctttggtc ggttatttcg taaatatcgc tctgtagctt atgcgttagt gctgaagcac
    10561 gttgggatct tgaaacaaaa tttttacctc gtagcaacta atatgggttt agctcctacg
    10621 gcggcagggg ttggggatag cgatgctttt gctcagataa cgggtttaga ctatctcgaa
    10681 gaatcggttg tcggagagtt tatgctcgga tcgttgccca gcagcaatgt tgaagcaagt
    10741 gggctggagt cgatggaggt ggatggctca gaagaacctg ctgaggcaac tgtctcagcc
    10801 agtgacacag gattggctgg aatggagctt gagcctgatt ctggtgaacc tgagcctggt
    10861 caagcagaaa tattgggaaa tcagcttgag tcagttgggt cagtggcaga aattgcctcg
    10921 gctgagatag gagaaagtcc tgaaaatacc tcagagcaag ttgaagagac ggaggctgag
    10981 gctgttgaat tgacggcttc agatgcgatc gcccagactc cagattcatc cagtgtcgct
    11041 gagggagaaa aatcagcagg ggtttgggcc agtgaagttg agagttctgc aacccaacta
    11101 tcgatcgcac tccatcccca tgacctggac gatcgcattc ccggactggc tgaacttcac
    11161 aaccaaacat taggcgatcc tcggatcacg attgtaattc tagatggcaa cccagaccac
    11221 acattatcct gttttgctgg agccaacgtt tctaaggtgt ttccctactg gcatgaacca
    11281 gccgatccaa tcagcccaga agattacgcc acctttcaag ccatccgaga ccaggggcta
    11341 aagggcaaag cgaaacaaga ggcacttgaa agtgctatac ccgacaccat aaatcgtgtt
    11401 gaactcaatg accatgcttg tcatgtcacc agtaccatcg tgggtcaaga acatagccct
    11461 gtttttggca ttgctcccaa ctgccgagtc atcaatatgc ctcacgatgc cgtagttacg
    11521 agcgacaatg gtatagctct gagtggctat aacgaggtgc tgtctcccct caatttagcc
    11581 cgtgcctttg agttagctat agagttgggt gcaaatgtta tccactgcgc tttttgtcgc
    11641 cccacccaaa ccggagaagg ggaagaaatc ctagttaaag cgattaaaaa atgtattgat
    11701 aacaatatct tgatcgtctc gccgacgggg aacaatttag gcgaatgctg gtgtatgcct
    11761 gctgtgctgc cgggaacttt ggcagtggga gcagcaaagg ttgatggtac gccctgccac
    11821 tttagtaact ggggcggcaa taatggagaa gagggtatcc ttgctcccgg tgaagatatt
    11881 ttaggggcgc aaccttgcac tgaaaaaccc gtgcgtttga caggaaccag tatggcggct
    11941 ccggtgatga cgggtattgc ggcgctgtta atgagtttgc aagtgcaaca gggcaaaccc
    12001 gttgatgctg aagccgtgcg gactgcactg ctcaatacgg caattccctg tgacccaaac
    12061 gttgtagaag aaccagaacg ctgtctacgc ggctttgtga atatccctgg ggcgatgaag
    12121 gtgctgtttg gacaaccttc tgttaccatt tcctttgcag gcgaccaagt gacccgcaca
    12181 gaatgggctg gctatggcac ggcgatcgca ggggatacgt cgagtattcc tgctgggatt
    12241 actgcgatcg cagtaagttc ggttggagac tctactatga caatgcaatc ggctacgcca
    12301 atggcggccg aagtcgcggt aactgcggcg gctgacccgg ctacggtgga ggctagtacc
    12361 gctttttcgg gccatgttta tgctctggga accttgagct atgactttgg cgatgaaacc
    12421 tgtgttgata ccttcaaaga acggatggct ccggtagaaa tggacagcat cttggttccg
    12481 ccagacccct acgatccccg tcagatggtt gaacacctcg atcgcaaccc agacgaaagc
    12541 cgttcgttga tctggacgtt gagcctggat ggagacacga tctatgtcct agagccaaca
    12601 ggagcgtttt cagaccagat ctacgagatg tttgtgctga tgttagtagg tcaactggaa
    12661 caaaaaagca gtgatgaatt tgtagaacga attagcatcc cagcccgaca aactaaccgt
    12721 acagttgaac tattctctgg tcaggttgtg cccgtcgtga aggtacatga tatccgagga
    12781 atgtacggct ggaaagtgaa taccctggtc aacgcggcga tggtggcgat ctctcgtcag
    12841 gtggacgaag cccaagcacc cctcatccaa caagctctga cttcgttcct caaccgagtc
    12901 tacaatgatc tacgcaatgt gggtcagact tcgcgcgatc gcgccctcaa cttcgccgcc
    12961 accaacatct tccaagcagc ttccgtcttt gcgaaagcga tcgccgagcg tcgtcaactc
    13021 gacactatag aagtcgaaaa aagccccttc tgtcggcgaa acagtgactg ttgggatatc
    13081 aagctagagt tctctgaccc agaaaggagt agtcggggac gcaaggtttt tctatttacc
    13141 ttggatgtag tgcttcagat gcccgtcact ttgggtaaaa tcaagagttg gtcaatgcct
    13201 tcgcgttctg aacaaacctg atctacaaca aaatgggcta ccaacctaag tgtcggcgta
    13261 aacagaaggt tcgtgtcacc aggtgcagcc ttgggaagtt ttgcaaaaat gacccatttt
    13321 ttcggcgaaa ttagacctct tgcaaaagtc ttaagtcgat ccttgtacag caacattttt
    13381 gaagattatc aactactaat aaataattaa ctgaaagtcc ctcccattat tgtttctatc
    13441 gtttgttttc ggatttatgc aagaggtctc ttgatgatag cagtcaaggc aaaaaatcga
    13501 ccatcaagaa aaaacgagcg atcaaagggc aagggatccg ccacagcttc ccccggcttt
    13561 taggcagaat agcatctaat accatttctc taaatgaaaa ctacacatcg aatttaaatc
    13621 cccctttaag ttcccctttt taagggggat ttagggggat taaacttctg tagtcagact
    13681 ttggagaatt ggtataatat agttatctaa ttaaattaga tgcatgatat agctaccaaa
    13741 agcacaggtt atgccctttt gtcaacgggg aagcccagat atactattaa agaatcatct
    13801 ccccgcagca aacaccatgc cagttaaagg ccaggccaag actataggaa tgc
//
