LOCUS       BGC0000474             12600 bp    DNA     linear   BCT 01-FEB-2008
DEFINITION  Microcystis aeruginosa PCC 7806 microcyclamide biosynthetic gene
            cluster, strain PCC 7806.
ACCESSION   BGC0000474
VERSION     BGC0000474
KEYWORDS    .
SOURCE      Microcystis aeruginosa PCC 7806
  ORGANISM  Microcystis aeruginosa PCC 7806
            Bacteria; Cyanobacteria; Oscillatoriophycideae; Chroococcales;
            Microcystaceae; Microcystis.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 19:37:46
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12600
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..12600
                     /clone="epifosPCC7806-mca1"
                     /db_xref="taxon:267872"
                     /mol_type="genomic DNA"
                     /organism="Microcystis aeruginosa PCC 7806"
                     /strain="PCC 7806"
     subregion       1..12600
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12600
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            74..2047
                     /gene="mcaA"
     CDS             74..2047
                     /codon_start=1
                     /db_xref="GOA:A8YE40"
                     /db_xref="InterPro:IPR000209"
                     /db_xref="InterPro:IPR015500"
                     /db_xref="InterPro:IPR022398"
                     /db_xref="UniProtKB/TrEMBL:A8YE40"
                     /gene="mcaA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 167; E-value: 1.4e-50)"
                     /gene_kind="biosynthetic"
                     /product="subtilisin-like protein"
                     /protein_id="CAP64335.1"
                     /sec_met_domain="cyanobactin_synth (E-value: 5.9e-240,
                     bitscore: 785.8, seeds: 40, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.6e-35, bitscore:
                     112.0, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDRLHTISQGDSGICVAVLDGPVDQAHPCFQGANLTYLPTLVQE
                     AAKVDGSMSMHGTHVASILFGQPGSPVQGIVPQCKGIIIPIFADDRRRTSQLDLARGIE
                     QAVHAGAHIINLSGGQLTDFGEADGWLKNAVRLCRENNVLLVAAAGNNGCECLHVPAAL
                     PSVLAVGAMDAQGKPLDFSNWGEAYQTEGILAPGEDILGAKAGGGTHRLSGTSFATPIV
                     SGVAGVLLSLQREQGDSPDPQKIRQILLQSALPCELDLPEESRRCLAGKLNVSGAITLL
                     KGEKMSEEFSSVAASEVTAAGCGCGGTLSATPQEQTAPSLTAVASSPSAGAVPSDTPNL
                     NNLIQSFPTAVTQQPTMPNFTANSVTASQAPSELAEMGQLVYALGTLGYDFGTEARRDS
                     FKQLMPPFDLGGGLMVPANPYDARQMVDYLGENISEARSLIWTLNIELTPVYAIDPAGP
                     FAAESYRALQELLAGQIQPENDGEYVERVSIPGILTGRKVKLFSGQVIPVIEPQSTRGL
                     YGWKVNNLVSAAMDAVQAEDGTADEDRIRKTLDGFLNRIYYDLRNLGTTSQDRALNFAV
                     TNAFQAAQTFSQAVAVGMELDSVTVEKSPFCRIDSDCWDVKLKFFDPENSRRAKKIFRF
                     TIDVSDLIPVTLGEVRSWSSPY"
     gene            2153..2404
                     /gene="mcaB"
     CDS             2153..2404
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A8YE41"
                     /gene="mcaB"
                     /product="putative microcyclamide biosynthesis protein"
                     /protein_id="CAP64336.1"
                     /transl_table=11
                     /translation="MRLPLLSPPIERPHFIQPGLCVDLENGRPEDLVHIRMDLLHAANY
                     NDPAAFANRSFNQVMHSSASQSSASQGWGGFGSMAGFY"
     gene            2455..2697
                     /gene="mcaC"
     CDS             2455..2697
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A8YE42"
                     /gene="mcaC"
                     /product="putative microcyclamide biosynthesis protein"
                     /protein_id="CAP64337.1"
                     /transl_table=11
                     /translation="MSKNKTTTTSSEVPSNPEPSKSEPSKSEPTTIATPEPKKSYVPTS
                     QTGLQDYSYWCQYVKDQAKKSDKDPKSFRRGRIWA"
     gene            2756..5086
                     /gene="mcaD"
     CDS             2756..5086
                     /codon_start=1
                     /db_xref="GOA:A8YE43"
                     /db_xref="InterPro:IPR003776"
                     /db_xref="InterPro:IPR016040"
                     /db_xref="InterPro:IPR022291"
                     /db_xref="UniProtKB/TrEMBL:A8YE43"
                     /gene="mcaD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Cyanobactin_D_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: NHLP_CD_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Goadsporin_CD_RRE"
                     /gene_kind="biosynthetic"
                     /product="adenylation/heterocyclization protein"
                     /protein_id="CAP64338.1"
                     /sec_met_domain="TIGR03604 (E-value: 1.8e-85, bitscore:
                     276.2, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 2.3e-99, bitscore: 322.4,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="Cyanobactin_D_RRE (E-value: 3.2e-44,
                     bitscore: 138.3, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="Goadsporin_CD_RRE (E-value: 1.4e-20,
                     bitscore: 62.6, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="NHLP_CD_RRE (E-value: 1.3e-22, bitscore:
                     69.1, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQSTPLLQIQPHFHVEVIEPKQVYLLGEQANYALTGQLYCQILPL
                     LDGQHSREQIVEKLDGEVPSEYIDYVLDRLAEKGYLTEAAPELSSEVAAFWSELGIAPP
                     VAAAALRQPVTLTPVGNISEVTVAALTTALRDIGISVQTSTEAGSPTALNVVLTDDYLQ
                     PELAKINKQALESQQTWLLVKPVGSVLWLGPVFVPGKTGCWDCLAHRLRGNREVEASVL
                     QQKQAQQQRNGQSGSVISCLPTARATLPSTLQTGLQFAATEIAKCIVKHHVNATAPGTV
                     FFPTLDGKIITFNHTVIDLKSHVLVRRPQCPSCGDRHRLHRQGFEPVKLVSRPKHFTHD
                     GGHRAFTPEQTVQKYQHLVSPITGVVTELVRLTDPANPLVHTYKAGHAFGSATTLRGLR
                     NTLKHKSSGKGKTDIQSRASGLCEAIERYSGIFQGDEPWKRATLAELGDLALHPESLLY
                     FSDTQYANREELNAQGSAAAYRWIPNRFDVSQAIDWTPVWSLTEQKHKYVPTAFCYYGY
                     PLPEEQRFCKADSNGNAAGNTLEEAILQGFLELVERDSIAMWWYNRIRRPAVDLSTFDE
                     PYFVDLQQFYQQQNRELWVLDVTADLGIPAFAGFSRRTVGTSERISIGFGAHLDPTIAI
                     LRALTEVSQVGLELDKIPDDKLDGESKDWMLNVTVENHPWLAPDPSVPMKTASDYPKRW
                     SDDIHTDVMNCVKTAQTAGLEVMVLDQTRPDIGLNVVKVIIPGMRTFWTRFGQGRLYDI
                     PVKLGWLDAPLAEEELNQTNIPF"
     gene            5203..5511
                     /gene="mcaE"
     CDS             5203..5511
                     /codon_start=1
                     /db_xref="InterPro:IPR022264"
                     /db_xref="UniProtKB/TrEMBL:B0BKB6"
                     /gene="mcaE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: TIGR03678"
                     /gene_kind="biosynthetic"
                     /product="microcyclamide precursor protein"
                     /protein_id="CAP64339.1"
                     /sec_met_domain="TIGR03678 (E-value: 8.9e-26, bitscore:
                     79.3, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRITPMDKKNLLPNQGAPVIRGISGKLPSHLAELSEEALGGNGAE
                     ASATVSICAFDGAEASFTGCMCAFDGAEASITGCICAFDGAEASITGCICAFDGDEA"
     gene            5806..6771
                     /gene="mcaF"
     CDS             5806..6771
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A8YE45"
                     /gene="mcaF"
                     /product="putative microcyclamide biosynthesis protein"
                     /protein_id="CAP74572.1"
                     /transl_table=11
                     /translation="MTLTSMLKNNHLKARRLQFLRGHQEAFDVEPTFMLSLFEEAVLGI
                     EETCGVESKCNVEKDQLFAIDFQVCNDQGRTWPMSLTHAVKFMDKIESTVGVRLNRNLL
                     EQFATLHMDSHKIENNTVGIDLRPKNEDSCIKVCLHLGSEEEPEELVRTALELDGGSYS
                     PELLQVLLKSTIVIGFNLFLNGYSDIELWALAPGEQYEITNSDRGKYLKHYIQRNFSPK
                     VNSLLKECTFFCVSFFHKKEPVIIFHYEDTKEIPKNFFFNSLGDRIYSFCQGQDCMTYA
                     GVAVTERELEKDRLENFSILYNQRDECKPLLHIKKREDFS"
     CDS             6824..7627
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:A8YE46"
                     /note="ORF1"
                     /product="hypothetical protein"
                     /protein_id="CAP64340.1"
                     /transl_table=11
                     /translation="MGIEFNHLYVTLDAETLDSIAKSEFISQEFCTISRDTVKTDTESW
                     TGIYLRGKHSYLELFPPGGAEGLREGFSGIGFNSQQSGEMDIVQEKLRPLLGAKEILSD
                     LQVRQTEAGKVPWFYYLSINPVEREAFASWLMEFHQDYLKYKNIKLTSDGCFNRAAYLK
                     TLDTSETCLFDDISEVHLELTLSEQEELALLLQAFGYDSSSAGDITTYHSHNFMIKVCH
                     KVARRYRICKVVCTLKEQLQQEKTFTFGKNAQLNVGRSLAIWEFG"
     CDS             7716..8063
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:B0BKB8"
                     /note="ORF2"
                     /product="hypothetical protein"
                     /protein_id="CAP64341.1"
                     /transl_table=11
                     /translation="MINNCPFAISLNPDVQIIDTKDGYTLHGAESEPNTWLTLAITSSK
                     LLFSFPTLLLVLPAELKIVESVFEYLLKCSVPRLNHLLTNGLIKCQELYLNQQNSLTTV
                     KGYHFHKCWPG"
     gene            8567..12508
                     /gene="mcaG"
     CDS             8567..12508
                     /codon_start=1
                     /db_xref="GOA:A8YE48"
                     /db_xref="InterPro:IPR000209"
                     /db_xref="InterPro:IPR000415"
                     /db_xref="InterPro:IPR015500"
                     /db_xref="InterPro:IPR020051"
                     /db_xref="InterPro:IPR022398"
                     /db_xref="UniProtKB/TrEMBL:A8YE48"
                     /gene="mcaG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 87.3; E-value: 2.2e-26)"
                     /gene_kind="biosynthetic"
                     /product="thiazoline oxidase/subtilisin-like protease"
                     /protein_id="CAP64342.1"
                     /sec_met_domain="cyanobactin_synth (E-value: 3.7e-181,
                     bitscore: 592.0, seeds: 40, tool: rule-based-clusters)"
                     /sec_met_domain="PF00881 (E-value: 2.9e-20, bitscore: 62.2,
                     seeds: 78, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.3e-21, bitscore:
                     66.3, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNCPFAISLNPAVQVTSTKDGYTLHGAESEPNAWLTVATTPGMG
                     MALDSLKAGNTTPELLVQGLAELEGQAAGEQFGQMLQQLDERGWLSYAVLPLAVVIPMV
                     DSAELNLTEPYWTQTRLSLSRFAYQHPYEGAMVLESPLSKFRVKLLDWRASAILAQLAQ
                     PQTLGSLTPPPYLGAETAYQFLNLLWATGFLTADPEPPSLRLWDFHNLLFHSRSRFGRH
                     DYLAIDHDIEQWSDFPVVKPPMSDQIVPLPRPNLGALISNDATLTEAIETRKSVRQYDD
                     NHPITIEQLGELLYRTARIKELLSAEDWFGKNWREQLSFEADIDYGELSRRPYPSGGTM
                     YELEIYPVVRLCEGLSQGIYHYDALNHQLEQVIVTQDDIFALSGYGLLDLLKLNGLGTP
                     QVLLVITARFGRLFRRYRSVAYALVLKHVGVLQQNLYLVATNMGLAPCAIGGGDSDAFA
                     QITGLDYLEESVVGEFMLGSLPNEDVKASGLESMEVDGSEEPAEATVSASETELAGMEL
                     EPDSGEPEPGQTEILGNQLESVGLVAEIASAEIGESPENTSEQVEETEAEAVELTASDA
                     IAQTPDSSSVAEGEKSAGVWASEVESSATQLSIALHPHDLDDRIPGLAELHNQTLGDPR
                     ITIVILDGNPDHTLSCFAGANVSKVFPYWHEPADPISPEDYATFQAIRDQGLKGKAKQE
                     ALESAIPDTINRVELNDHACHVTSTIVGQEHSPVFGIAPNCRVINMPHDAVVTSDNGIA
                     LSGYNEVLSPLNLARAFDLALELGANIIHCAFCRPTQTGEGEEILVKAIKKCIDNNILI
                     VSPTGNNLGECWCMPAVLPGTLAVGAAKVDGTPCHFSNWGGNNGEEGILAPGEDILGAQ
                     PCTEKPVRLTGTSMAAPVMTGIAALLMSLQVQQGKPVDAEAVRTALLNTAIPCDPNVVE
                     EAERCLRGFVNIPGAMKVLFGQPSVTISFAGDQVTRTEWAGYGTAIAGDTSSIPAGITA
                     IAVSSVGDSTMTMQSATPMAAEVAVTAAADPATVEASTAFSGHVYALGTLSYDFGDETC
                     VDTFKERMAPVEMDSILVPPDPYDPRQMVEHLDRNPDESRSLIWTLSLDGDTIYVLEPT
                     GAFSDQIYEMFVLMLVGQLEQKSSDEFVERISIPARQTNRTVELFSGQVVPVVKVHDIR
                     GMYGWKVNTLVNAAMVEISRQVDEAQAPLIQQALTSFLNRVYNDLRNVGQTSRDRALNF
                     AATNIFQAASVFAKAIAERRQLDTIEVEKSPFCRRNSDCWDIKLEFSDPERSSRGRKVF
                     LFTLDVVLQMPVTLGKIKSWSMPSRSEQT"
ORIGIN
        1 aatcacaatt tttgaggtac cattattttg tcaagaatct aggctacata agtaaaaatg
       61 ataacacctt taaatgctag atagattaca taccatctcc caaggagata gcggtatttg
      121 tgtggctgtt ttggatgggc ctgttgatca agctcaccct tgcttccaag gcgcaaacct
      181 tacctatctg cccacattgg tgcaagaagc cgctaaagtt gatggcagta tgtccatgca
      241 cggaactcac gttgccagca ttctctttgg gcaacccggt agccccgttc agggaatcgt
      301 acctcaatgc aaaggcatta tcataccgat ttttgccgat gaccgtcgcc gaacttccca
      361 gctagattta gctcggggaa tcgagcaagc cgttcatgcc ggagcccaca tcatcaacct
      421 cagtgggggt caactcactg actttggtga agccgatggc tggttaaaaa atgctgttcg
      481 tttgtgccga gagaataacg tcttgttagt ggcagcagca ggcaataatg gttgcgagtg
      541 tttgcacgtt ccggcagctt taccgtcagt gctggccgtc ggggcaatgg atgcccaggg
      601 gaaacccctc gactttagca actgggggga agcctaccaa actgaaggca tcctggctcc
      661 aggggaagat attctgggag ccaaagctgg ggggggaacc caccgattaa gtggaaccag
      721 ttttgccact cctatcgttt ctggtgtcgc tggggtatta ctcagccttc aacgggaaca
      781 gggagattcc cccgaccccc aaaaaattcg tcaaattttg ttgcagagtg ctttaccctg
      841 tgaacttgac ttgccagaag aaagccggcg gtgtctggcg ggcaaactta atgtctctgg
      901 agcaatcacg ttactaaaag gagaaaaaat gtctgaggaa ttttcgtctg tggcggcatc
      961 agaagtaaca gcagcaggtt gtggttgtgg aggcacatta agtgctaccc cccaagagca
     1021 aacggctcct agcttaactg ctgtggcttc cagtccatcg gctggggctg tccccagcga
     1081 cactcccaac ttaaacaact taattcaatc attccctact gctgtaactc aacaaccaac
     1141 tatgcccaac ttcactgcta attccgttac tgccagccaa gctcctagcg aactcgccga
     1201 gatgggtcag cttgtttatg ctctaggaac ccttggttat gacttcggga ctgaagcccg
     1261 tcgggactcg tttaaacaac tcatgcctcc ctttgattta ggtggaggac tcatggttcc
     1321 cgccaacccc tacgacgccc gtcagatggt ggactatcta ggggaaaaca tctccgaggc
     1381 gcgatcgctg atttggacat taaacattga actgactccg gtttatgcga ttgatccggc
     1441 tggccccttc gccgccgagt cctaccgcgc ccttcaagaa ctcctggcgg gtcaaatcca
     1501 accggaaaac gatggcgaat atgttgaacg ggtaagtatc cccggtattt taactggacg
     1561 taaggttaaa ctcttctccg gtcaagtcat cccggtgatt gaaccccaaa gcacccgtgg
     1621 tctctacggt tggaaagtca acaacctggt cagcgcagcg atggacgcgg tacaagctga
     1681 agatggcact gctgacgaag accggattcg caaaaccttg gatggcttcc tcaaccgcat
     1741 ctactacgac ctccgcaact tgggaaccac ttcccaagac cgcgccctta actttgccgt
     1801 caccaacgcc ttccaagccg cccaaacttt cagccaagcg gtagccgttg gcatggaact
     1861 cgatagcgtt acggtagaga aaagcccctt ctgccggatc gatagcgact gctgggatgt
     1921 gaagctgaag ttcttcgacc cggaaaacag ccgtcgggct aagaagattt tccgctttac
     1981 cattgatgta agtgacctaa ttcctgtcac cctcggcgaa gttcgttctt ggtcttctcc
     2041 ctactaagga gccgcacaat tttagtgaga gtgcaatatt agaccccact ctcactagga
     2101 gatgcagatg tgttcagtta attaatactc tccataggga gaatcctaaa ctatgcgact
     2161 tcctctatta tctcccccga ttgaacgtcc ccatttcatc cagccaggac tgtgcgtgga
     2221 tctagaaaat ggtcgtcccg aagatttggt gcatattcgg atggatctgc tccatgccgc
     2281 taactataac gatcctgcag cctttgccaa ccgtagtttc aaccaagtga tgcactctag
     2341 tgccagccaa agttcagcca gccagggctg gggcggcttt ggcagcatgg caggattcta
     2401 ttaatgggct gctaatcact cgccctcagc caaccctaaa catccaagga cactatgagc
     2461 aagaacaaaa ccacaacaac atcctcggaa gtcccctcta accctgaacc gagcaagtct
     2521 gaaccgagca agtctgaacc gacgacgatc gcaacgcctg aacccaaaaa gtcttacgtt
     2581 cccacttccc agaccgggct gcaagattat tcctactggt gccaatacgt caaggatcaa
     2641 gccaagaaat cagacaaaga ccctaaatcc ttccggcgcg gtcgcatttg ggcttaaccc
     2701 ctagctgctg ctgctggtct tgttatcgtc cacttaatca ctgactgttc gttctatgca
     2761 atccacccca ctgctccaaa ttcaacccca ctttcatgtc gaggtgatcg agccaaagca
     2821 agtctatctc cttggcgaac aggctaacta tgccctgact ggacagcttt actgccagat
     2881 tttgcccctc ttggatgggc aacacagccg tgagcagatt gtcgagaaac tggatgggga
     2941 agtcccatca gaatacatcg actatgtact cgatcgcctc gctgagaaag gttatctgac
     3001 tgaggcagcc ccggaactgt cctcggaagt ggcggcattt tggagcgagt tagggattgc
     3061 acctccggtt gctgcggcag ctttacggca gcctgtaacc ctaaccccag tgggcaacat
     3121 cagtgaagtg acggtagcag ccctgacaac ggctctgcgg gacattggta tttccgtgca
     3181 gacctcaact gaagcaggtt ctccaacggc gctcaacgtc gtcttaaccg atgactattt
     3241 gcagcccgaa ctcgccaaga ttaataaaca ggcactagag agtcaacaaa cttggctact
     3301 ggtcaaacct gtaggcagtg tgctgtggct ggggcctgtg tttgttccgg gcaagacggg
     3361 gtgttgggac tgtctagctc atcggctacg gggcaaccgg gaagtggagg catcggtgtt
     3421 gcagcaaaaa caagcccagc agcaacgtaa tggtcagtcc ggttctgtaa tcagttgcct
     3481 gcctaccgca cgggctaccc tgccttctac cctgcaaacg gggctacagt tcgcggcaac
     3541 tgaaattgcc aagtgcattg tcaagcacca cgtcaatgcc actgcaccgg gtactgtgtt
     3601 ctttcccact ctggacggca agattatcac ctttaatcac acagttatcg acttgaaaag
     3661 tcatgttctc gtccgacggc cccaatgtcc tagctgtggt gatcgccaca ggttgcaccg
     3721 gcaaggcttt gaacccgtaa aactggtatc ccgtcctaaa cactttaccc atgatggcgg
     3781 tcatcgtgcg tttaccccag agcagaccgt acagaaatat caacatctgg tcagcccaat
     3841 tactggggta gtaacagaac tggtcagact caccgatccg gctaatcctt tggtgcatac
     3901 ctacaaggca ggtcatgcct ttggcagtgc tacaactctg cggggtttac ggaatactct
     3961 gaaacacaag agttctggta agggtaaaac tgatattcaa tcccgtgcca gtgggttgtg
     4021 tgaagccata gaacgctatt cgggcatttt tcagggagat gaaccctgga aacgggcaac
     4081 cctggcggaa ctgggagact tagccttaca tcctgagtca ttgctgtact ttagcgacac
     4141 ccaatatgcc aaccgggaag agttaaatgc tcagggttca gccgccgctt atcgctggat
     4201 tcctaaccgt tttgatgtta gtcaagcgat cgactggaca cctgtatggt cattgacaga
     4261 gcagaagcat aaatatgtcc ctactgcctt ttgctactac ggttatcctt tgcccgaaga
     4321 acagcgcttc tgtaaagcag attctaacgg taatgctgcg ggtaatacct tagaagaggc
     4381 gatcctgcaa ggatttctgg aactggtaga gcgggacagt atcgctatgt ggtggtacaa
     4441 ccggatacgc cgtcctgccg tagatctgag tacctttgat gaaccctatt ttgttgattt
     4501 acagcaattc tatcagcaac aaaatcggga gttatgggtg ttggatgtaa ctgctgatct
     4561 agggattcca gcctttgcag ggttttcacg gcgcacggtg ggtacttcag aacggatctc
     4621 catcggcttc ggggcgcacc tcgatccgac tattgcgatt ctccgcgccc taactgaagt
     4681 cagtcaagtg ggattagagt tagacaaaat accggacgat aagcttgatg gcgagtccaa
     4741 ggactggatg ctgaatgtca cagtagaaaa tcatccttgg ttagcgcccg atccctctgt
     4801 gcccatgaaa actgccagtg attacccgaa acgttggagt gatgatattc acaccgatgt
     4861 gatgaattgt gtcaaaactg cccaaacagc aggactagag gtgatggtct tagaccaaac
     4921 gcgaccggac atcggtttga atgtggtcaa ggtgattatc ccaggaatgc gaactttttg
     4981 gacgcggttt ggtcagggac ggctctatga catacccgta aagctgggat ggctcgatgc
     5041 gccactagct gaagaggaat tgaaccagac caacatcccc ttttaagttt tttaaagttt
     5101 aggggcgacg gatttctcta catatccttg ccccaccaag cttcttccca aagaagaagg
     5161 ggactgaccc acccctaacg ggttaacaaa gcaaacaaca agatgaggat tactccaatg
     5221 gataagaaaa accttctgcc caaccaaggc gcacctgtca tccgtggcat cagtggtaaa
     5281 ctccccagcc acttagctga actctctgag gaagctctcg gtggtaatgg tgcagaagca
     5341 tctgctactg ttagtatctg cgctttcgat ggtgctgaag catctttcac tggctgcatg
     5401 tgcgctttcg atggtgctga agcatctatc actggctgca tttgcgcttt cgatggtgct
     5461 gaagcatcta tcactggctg catttgcgct ttcgatggtg acgaagccta aggatagcct
     5521 ataccaaatc tgtgagaata gggtctatcc gactgattag cctatctcaa ccggatttag
     5581 tgtattcgtt tatttgagaa aacgaggtta aagatgttga gtcgcactcc aaactttttc
     5641 ctagattggg taagattttc tcatctacct cgttttcttt taattgggtt tttaagttct
     5701 cattgtaccc aattggacac gacttacgca gccacagcac ctgtaggggc aatcccctta
     5761 ccattggtat aaagggacta aaacccaaca acaaacctac tggaaatgac cctaacttct
     5821 atgctgaaaa ataatcatct caaagcccgt cgtcttcagt ttttgcgggg ccatcaagag
     5881 gcttttgatg tggagccaac ctttatgctt tcgctgtttg aagaagccgt ccttggcata
     5941 gaagaaactt gtggtgttga atctaaatgt aatgtggaaa aagatcaatt attcgccatt
     6001 gattttcagg tttgtaatga tcaaggacgt acttggccaa tgtctttgac ccatgcggtc
     6061 aagtttatgg ataaaattga aagtacagta ggtgttcgac ttaatcgtaa tttgctggag
     6121 cagtttgcca cattacacat ggactctcat aaaatagaga acaacacagt cggaattgac
     6181 ctaagaccga aaaatgagga ttcttgtata aaagtatgtc tgcatcttgg ctcagaagaa
     6241 gagcctgaag aattagtaag aacagcactc gaacttgatg ggggtagcta ttcacctgaa
     6301 ctcctacagg tattactcaa aagcactatt gtaatagggt ttaatctttt cttgaatggc
     6361 tacagtgata ttgagttatg ggctcttgct ccgggagaac aatatgaaat cactaattcc
     6421 gacagaggca aatatttaaa acattatata cagaggaatt tttcaccgaa agttaattct
     6481 ttgcttaaag aatgcacctt tttttgtgtt tctttttttc ataaaaaaga gccagttata
     6541 atatttcatt atgaggatac taaagagata cctaagaact tcttctttaa cagtttaggg
     6601 gatagaatct acagcttttg tcagggtcaa gattgtatga cttatgctgg agtagctgtc
     6661 acagagcggg aattggaaaa agacaggtta gaaaacttta gtattcttta taatcaacgg
     6721 gatgaatgta aacctttgtt gcatattaaa aaacgagaag attttagtta gtcttctccc
     6781 cctctccccg tctctacttt agttatgctt actgcctaag aatatgggaa tagaatttaa
     6841 ccatttatat gttaccctcg acgcagaaac cctagattca attgccaaat cagagtttat
     6901 tagccaagaa ttctgcacaa tatccagaga tactgtaaaa actgatacag aaagttggac
     6961 gggaatttat ctcaggggaa aacattccta tcttgagctt tttcctcccg gcggtgctga
     7021 agggttaaga gaaggttttt ccggtattgg ttttaatagt caacaatcag gagagatgga
     7081 tattgtacag gaaaagctta gacccttatt aggagctaaa gaaatacttt ctgatttgca
     7141 agttcgtcaa accgaagctg gaaaagttcc ctggttttat tatttgtcca tcaacccagt
     7201 agagagagaa gcttttgctt catggttgat ggaatttcat caggattatt taaagtataa
     7261 aaacatcaaa cttaccagtg atggttgctt taaccgtgct gcttatctaa aaacccttga
     7321 tacttccgaa acctgtttat ttgatgacat atcagaagtt catcttgaat tgactttatc
     7381 ggaacaggag gagttggcat tacttcttca agcttttggc tatgactcat ctagcgcagg
     7441 agatattact acctatcatt cccataattt tatgatcaaa gtatgccata aagttgctcg
     7501 ccgttatcga atttgcaagg ttgtttgcac tctcaaagag caattacagc aggaaaaaac
     7561 ctttactttt ggtaaaaatg cacagttaaa tgtgggtcgc tcgttagcaa tttgggagtt
     7621 tggatgactc aagcaatcca taaaaattaa gacaaatttc accaattgct gttttcattg
     7681 ctccaccgat ttaataccgt ttcctattat taatcatgat caacaattgt ccttttgcta
     7741 tttcccttaa ccctgatgtc caaatcatcg acactaaaga tggctatacc ctgcacggag
     7801 cagaatctga gccgaatact tggctaacct tagcaataac gtcttcaaaa ctgctcttct
     7861 ctttccctac tcttcttcta gtgcttcctg ctgaattgaa aatcgttgaa tccgtctttg
     7921 aatatctgct aaaatgctcc gtacctcgtt taaatcactt gttaacaaat ggtctgataa
     7981 aatgtcaaga actatatcta aatcaacaga atagtttaac aacagtaaaa ggatatcatt
     8041 ttcataaatg ctggcccggc taaattgtcc ttgacgatag agagatggca aggcataaca
     8101 ctttaaaatt aggagtccta ccactgtgac acatcgaact tttcggtttc caaagatctg
     8161 ctctgctaca aactcttgcg atatttttcg gaagagctta ttttgggtta acaatacgtt
     8221 aacttgtaac ccatcaaatc ttccccgaag aaaattttta tcttcactct ggataccaat
     8281 ctctggtaaa gtgtcaagat cggcacgagc caggatcagg tcaatatctt gagtattgcg
     8341 accctccaca tagctgagta gagctacccc gccaacgaga acataacgaa tttctcgttc
     8401 agcaagcgca tcaaataatc gttcaacagt ctgaaaaaaa tcagaacttt ctgacattac
     8461 accaccccaa ttcttaacat caaataagat tccatttctg ataatttcac cgatcgctgc
     8521 tgtcattgat tcactgccca atcatcgttc tccattatcc atcaatatga acaattgccc
     8581 ttttgctatt tcccttaacc ctgctgtcca agtcaccagc actaaagacg gttataccct
     8641 gcacggagca gaatctgagc cgaatgcttg gctaaccgtt gccacaacgc ccggaatggg
     8701 tatggctctt gatagtctca aggcaggcaa caccacccct gaattactcg tgcaagggtt
     8761 agccgaactc gagggtcagg cagccgggga acagtttggg cagatgctac aacaattgga
     8821 tgagcgaggt tggttgagtt atgccgtgtt acccctagca gtggtgattc cgatggtgga
     8881 ttccgccgag ttgaatctca ccgaacccta ctggacacaa acccgtctca gcctctcccg
     8941 ttttgcctat cagcatccct acgaaggcgc gatggtattg gagtcccctc tgtcgaagtt
     9001 tcgggtcaag ctccttgact ggcgagccag tgcgatctta gctcaactgg cccagcccca
     9061 aactttagga tcactgacac caccgccgta cttgggtgca gaaaccgcct atcagtttct
     9121 caacctgctg tgggcaacgg ggtttctgac ggctgacccc gaacccccct ccctccggct
     9181 ttgggacttc cataacctgc tgttccatag tcgcagtcgc ttcggtcgcc acgactatct
     9241 cgccatagac catgatatag agcagtggtc agattttcca gtcgtgaagc ctcccatgag
     9301 tgaccagatt gtgccactac cacgtccgaa cttgggggcg ttgatatcta atgatgctac
     9361 cctcacagaa gcgatcgaga cacggaaatc cgttaggcaa tatgatgata accatccgat
     9421 cactattgaa caactgggag agctacttta tcgcactgcc cgtattaagg aattgctttc
     9481 cgcagaagat tggtttggga aaaattggcg ggaacaatta agttttgaag cagatattga
     9541 ctatggggag ttaagccgtc gcccttatcc gagtggtggg acaatgtatg aattagaaat
     9601 ttacccagta gtacggcttt gtgaaggctt gagtcaagga atctaccact acgacgcact
     9661 aaatcaccag ctagagcagg ttattgtcac ccaagacgac atttttgctc tatcaggcta
     9721 tggtctgcta gacttattaa aattaaacgg attaggaaca ccacaggtac ttctagtaat
     9781 tacagcccga tttggtagac tattccgtag atatcgctct gtagcttatg cgttagtgct
     9841 gaagcacgtt ggagtattac agcaaaatct ctacctagtg gcgacgaata tgggattagc
     9901 tccctgcgcg attgggggag gtgatagcga tgcttttgct cagataacgg gtttagacta
     9961 tctcgaagaa tcggttgtcg gagagtttat gctcggatcg ttgcctaacg aagatgtcaa
    10021 agcaagtggg ttggagtcga tggaggtgga tggctcagaa gaacctgctg aagcaactgt
    10081 ctcagctagt gagactgaat tggctggaat ggagcttgag cctgattctg gtgaacctga
    10141 gcctggtcaa acagaaatat tgggaaatca gcttgagtca gttgggttag tggcagaaat
    10201 tgcctcggct gagataggag aaagtcctga aaatacctca gagcaagttg aagagacgga
    10261 ggctgaggct gttgaattga cggcttcaga tgcgatcgcc cagactccag attcatccag
    10321 tgtcgctgag ggagaaaaat cagcaggggt ttgggccagt gaagttgaga gttctgcaac
    10381 ccaactatcg atcgcactcc atccccatga cctggacgat cgcattcccg gactggctga
    10441 acttcacaac caaacattag gcgatcctcg gatcacgatt gtaattctag atggcaaccc
    10501 agaccacaca ttatcctgtt ttgctggagc caacgtttct aaggtgtttc cctactggca
    10561 tgaaccagcc gatccaatca gcccagaaga ttacgccacc tttcaagcca tccgagacca
    10621 ggggctaaag ggcaaagcga aacaagaggc acttgaaagt gctatacccg acaccataaa
    10681 tcgtgttgaa ctcaatgacc atgcttgtca tgtcaccagt accatcgtgg gtcaagaaca
    10741 tagccctgtt tttggcattg ctcccaactg ccgagtcatc aatatgcctc acgatgccgt
    10801 agtgacgagc gacaatggta tagctctgag tggctataac gaggtgctgt ctcccctcaa
    10861 tttagcccgt gccttcgact tagccttaga gttgggtgca aatattatcc actgcgcttt
    10921 ttgtcgcccc acccaaaccg gagaagggga agaaatccta gttaaagcga ttaaaaaatg
    10981 tattgataac aatatcttga tcgtctcgcc gacggggaac aatttaggcg aatgctggtg
    11041 tatgcctgct gtgctgccgg gaactttggc agtgggagca gcaaaggttg atggtacgcc
    11101 ctgccacttt agtaactggg gcggcaataa tggagaagag ggtatccttg ctcccggtga
    11161 agatatttta ggggcgcaac cttgcactga aaaacccgtg cgtttgacag gaaccagtat
    11221 ggcggctccg gtgatgacgg gtattgcggc gctgttaatg agtttgcaag tgcaacaggg
    11281 caaacccgtt gatgctgaag ccgtgcggac tgcactgctc aatacggcaa ttccctgtga
    11341 cccaaacgtt gtagaagaag cagaacgctg tctacgcggc tttgtgaata tccctggggc
    11401 gatgaaggtg ctgtttggac aaccttctgt taccatttcc tttgcaggcg accaagtgac
    11461 ccgcacagaa tgggctggct atggcacggc gatcgcaggg gatacgtcga gtattcctgc
    11521 tgggattact gcgatcgcag taagttcggt tggagactct actatgacaa tgcaatcggc
    11581 tacgccaatg gcggccgaag tagcggtaac tgcggcggct gacccggcta cggtggaggc
    11641 tagtaccgct ttttcgggcc atgtttatgc tctgggaacc ttgagctatg actttggcga
    11701 tgaaacctgt gttgatacct tcaaagaacg gatggctccg gtagaaatgg acagcatctt
    11761 ggttccgcca gacccctacg atccccgtca gatggttgaa cacctcgatc gcaacccaga
    11821 cgaaagccgt tcgttgatct ggacgttgag cctggatgga gacacgatct atgtcctaga
    11881 gccaacagga gcgttttcag accagatcta cgagatgttt gtgctgatgt tagtaggtca
    11941 actggaacaa aaaagcagtg atgaatttgt agaacgaatt agcatcccag cccgacaaac
    12001 taaccgtaca gttgaactat tctctggtca ggttgtgccc gtcgtgaagg tacatgatat
    12061 ccgaggaatg tacggctgga aagtgaatac cctggtcaac gcggcgatgg tggagatctc
    12121 tcgtcaggtg gacgaagccc aagcacccct catccaacaa gctctgactt cgttcctcaa
    12181 ccgagtctac aatgatctac gcaatgtggg tcagacttcg cgcgatcgcg ccctcaactt
    12241 cgccgccacc aacatcttcc aagcagcttc cgtctttgcg aaagcgatcg ccgagcgtcg
    12301 tcaactcgac actatagaag tcgaaaaaag ccccttctgt cggcgaaaca gtgactgttg
    12361 ggatatcaag ctagagttct ctgacccaga aaggagtagt cggggacgca aggtttttct
    12421 atttaccttg gatgtagtgc ttcagatgcc cgtcactttg ggtaaaatca agagttggtc
    12481 aatgccttcg cgttctgaac aaacctgatc tacaacaaaa tgggctacca acctaagtgt
    12541 cggcgtaaac agaaggttcg tgtcaccagg tgcagccttg tgaagttttg caaaaatgac
//
