LOCUS       BGC0000479             13422 bp    DNA     linear   BCT 08-MAY-2011
DEFINITION  Planktothrix agardhii NIES-596 prenylagaramide gene cluster,
            complete sequence.
ACCESSION   BGC0000479
VERSION     BGC0000479
KEYWORDS    .
SOURCE      Planktothrix agardhii NIES-596
  ORGANISM  Planktothrix agardhii NIES-596
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Oscillatoriales; Microcoleaceae; Planktothrix.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:43:14
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 13422
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(1..198)
                     /gene="pagC"
     CDS             complement(1..198)
                     /codon_start=1
                     /gene="pagC"
                     /product="hypothetical protein"
                     /protein_id="AED99424.1"
                     /transl_table=11
                     /translation="MSEEKKTTAKNKVTKSVGKPEKKEIQQDCMMTGLSDYGFWSQKVK
                     EEVAARKEPDAPFRRGRIWC"
     source          1..13422
                     /db_xref="taxon:443922"
                     /mol_type="genomic DNA"
                     /organism="Planktothrix agardhii NIES-596"
                     /strain="NIES-596"
     misc_feature    1..13422
                     /note="prenylagaramide gene cluster"
     protocluster    1..13422
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[521:13422]"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      522..13422
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
     cand_cluster    1..13422
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /kind="single"
                     /product="cyanobactin"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..13422
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000479"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..13422
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="cyanobactin"
                     /region_number="1"
                     /rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(246..455)
                     /gene="pagB"
     CDS             complement(246..455)
                     /codon_start=1
                     /gene="pagB"
                     /product="hypothetical protein"
                     /protein_id="AED99425.1"
                     /transl_table=11
                     /translation="MRLPNLSPPVKRPDVIYPHRSVDVVNGTEEELLSIRLKLLHGANY
                     NDPAAFAHRSYQQLKSSGSCGCGF"
     gene            complement(522..2489)
                     /gene="pagA"
     CDS             complement(522..2489)
                     /codon_start=1
                     /gene="pagA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 171.8; E-value: 4.9e-52)"
                     /gene_kind="biosynthetic"
                     /product="N-terminal cyanobactin protease"
                     /protein_id="AED99426.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 2.4e-38, bitscore:
                     122.6, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 3.3e-238,
                     bitscore: 781.4, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNIPGLKKLWSETRGDPKICVAVLDGIVDQNHPCFIGADLTRLP
                     SLVSGEANANGSMSTHGTHVASIIFGQHDSPVTGIAPQCRGLIVPVFADESLKLSQLDL
                     SRAIEQAVNNGANIINVSAGQLTDAGEADTWLEKAIQLCQENNVLLIAATGNDGCECLH
                     VPASLPTVLAVGAMDDQGKPVDFSNWGDAYQKQGILAPGKDILGAKPNGGTIRLSGTSF
                     ATPIVSGVAALLLSLQIKRGEKPDPQKVKNALLASATPCNPKDTDDQSRCLMGKLNILD
                     AIEHLTGETMSEDLELESVEASGCGCQDTQINESELDNQLETASEIKPDEIVASVTTQT
                     PINIPSFPQQTTNQPTMSNRTSNFVTASEAPSELEGKNLVYALGVLGYDFGSEARRDSF
                     KQLMPGVSIEGTMIPANPYDARQMVDYLGENLPEAKALIWTLNLELTPIYAIEPVGGFS
                     RDVYEVLQGLLSGQIQEENSPEFVQRVSIPGVLTGRSVKLFSGQVVPVIEINNTRGLYG
                     WKVNSLVSAAIESVQSEAGDAQEDAIRRTLSSFLNRIYYDLRNLGTTSQDRALNFASTN
                     AFQAAQTFAQAVGAGYELDSITVEKSPFCRLDSDCWDVKLKFFDPENSRRAKKIYRFTI
                     DVSDTIPVTLGEVRSWSSAY"
     CDS             complement(2608..2997)
                     /codon_start=1
                     /note="Orf1"
                     /product="conserved hypothetical protein"
                     /protein_id="AED99427.1"
                     /transl_table=11
                     /translation="MTELYNRNSEKEKRQFLRNNMTPAEQKLWLQLKGKQLENCKFRRQ
                     YSVAQFIIDFYSPEIKLAIEVDGDSHFQEGVQEYDQERQQFIESARITFLRFTNDDVYD
                     NISAVCEIIRDKIQSLRNLPEAPPY"
     CDS             complement(3295..3873)
                     /codon_start=1
                     /note="Orf2"
                     /product="conserved hypothetical protein"
                     /protein_id="AED99428.1"
                     /transl_table=11
                     /translation="MLITKPRFQTFAEYLQYDDNSEKLCELFNGELVEMPPESGLNFQI
                     ANRLFLVFALILGTDRVRGHGLELEVRGEPKNRYPNLTIIREEHIQLLSKRNTILLSMS
                     PPLLVIEVVSPGEIQRERDYIAKKLQYQDCSIPEYWIVDPQTQTILVLELTGDTYREIG
                     NFTNDDLVASPGLKELNLKVSEVFNSTSL"
     gene            complement(3873..4775)
                     /gene="pagF"
     CDS             complement(3873..4775)
                     /codon_start=1
                     /gene="pagF"
                     /product="putative prenyl transferase"
                     /protein_id="AED99429.1"
                     /transl_table=11
                     /translation="MIVNVIQKDRLKEQKLQFIRNHQQAFDVEPIYPLPLFEDFVTSIE
                     GDCSLEASCKIESDKLIASRFLLFFEDKTQEWQKYLHQSLTFFGLVENRVGVKINYSLL
                     QQFLGSSFDFSKVTVLSAGIDLRNNLAESSLKMHIRIKDYPEKLDKAFALSDGAADGNY
                     LKDFVNLIGFDFYFNGKSEIEIYAEVQEDDFFKPEINNLVWQHFPKTALQPLKASSLFF
                     TGLSKANNNPVLYYHLKNRQDLTNYFKLNDTAQRVHSFYQHQDILPYMWVGTAQKELEK
                     TRIENIRLYYYKSFKMESN"
     CDS             4806..5621
                     /codon_start=1
                     /note="Orf3"
                     /product="transposase"
                     /protein_id="AED99430.1"
                     /transl_table=11
                     /translation="MPRKPRNLQSGYSYHITIRCNNREFKLQKRECREVFLYAIKKALN
                     KYNFKLYALCIMSNHVHYLIEPAQPEDLPKIMHFLNWYTAMCFNRMLRRTGHFWEKRYF
                     SDGFPASDKDRALNTLRYIHGNPKAAKMRSGFFYDFSNYGSYERLTDDGLTQWHPAFLQ
                     LGNSLEDCAKKYKGFCQRYKPKEKKTPTRCHWGSKLLAGVHLSEKGSTTNPQKSKSVLT
                     SYYQPLTPKKSKSRLVQTPCQVSESPELTRIVSQFIEANRAPRETEKDF"
     gene            complement(5655..5807)
                     /gene="pagE7"
     CDS             complement(5655..5807)
                     /codon_start=1
                     /gene="pagE7"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_precursor"
                     /gene_kind="biosynthetic"
                     /product="prenylagaramide C precursor peptide"
                     /protein_id="AED99431.1"
                     /sec_met_domain="cyanobactin_precursor (E-value: 1.0,
                     bitscore: 1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKKNLKPQQAAPVQREINTTSSESGTSTGLTPHQAYLGIPLPFA
                     GDDAE"
     gene            complement(6095..6241)
                     /gene="pagE6"
     CDS             complement(6095..6241)
                     /codon_start=1
                     /gene="pagE6"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_precursor"
                     /gene_kind="biosynthetic"
                     /product="prenylagaramide B precursor peptide"
                     /protein_id="AED99432.1"
                     /sec_met_domain="cyanobactin_precursor (E-value: 1.0,
                     bitscore: 1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKKNLKPQQAAPVQREINTTSSESGTSTGLTPHINPYLYPFAGD
                     DAE"
     gene            complement(6405..6551)
                     /gene="pagE5"
     CDS             complement(6405..6551)
                     /codon_start=1
                     /gene="pagE5"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_precursor"
                     /gene_kind="biosynthetic"
                     /product="cyanobactin precursor peptide"
                     /protein_id="AED99433.1"
                     /sec_met_domain="cyanobactin_precursor (E-value: 1.0,
                     bitscore: 1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKKNLKPQQAAPVQREINTTSSEAGTGLTPLRSIGDFRHPFAGD
                     DAE"
     gene            complement(6835..7032)
                     /gene="pagE4"
     CDS             complement(6835..7032)
                     /codon_start=1
                     /gene="pagE4"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_precursor"
                     /gene_kind="biosynthetic"
                     /product="cyanobactin precursor peptide"
                     /protein_id="AED99434.1"
                     /sec_met_domain="cyanobactin_precursor (E-value: 1.0,
                     bitscore: 1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKKNLKPQQAAPVQREINTTSSVGGTGLTPQIGFSINKIRDDSA
                     GLFSWYDPFSFDSNIRLKKY"
     CDS             7209..7535
                     /codon_start=1
                     /note="Orf4"
                     /product="conserved hypothetical protein"
                     /protein_id="AED99435.1"
                     /transl_table=11
                     /translation="MMKKVIQSTGKIDRVGQLSLDYPIKGTPPSSVRVIILWEETETEV
                     NDFWEQIKEYQQYPLMSAEQLQLELKQSLTEAGYDSREKIVDLVQDIKREISQERQQKQ
                     DSIQ"
     CDS             7532..7789
                     /codon_start=1
                     /note="Orf5"
                     /product="conserved hypothetical protein"
                     /protein_id="AED99436.1"
                     /transl_table=11
                     /translation="MTKIPELIFLDTNVYLIGAVERQSPEGLILKWLGWEAPNDNSVGV
                     IISEELIDQIARVANLQSIGLIFPIESVYEDIIFEELGTN"
     CDS             8087..8314
                     /codon_start=1
                     /note="Orf6"
                     /product="conserved hypothetical protein"
                     /protein_id="AED99437.1"
                     /transl_table=11
                     /translation="MTHLLEKAFQEAQKLSNYLQDELAQQLLEDIENELKWQESLSNPD
                     MDLGIFQEMAQLALLEDEEGKTEDKGFGEK"
     CDS             8314..8589
                     /codon_start=1
                     /note="Orf7"
                     /product="conserved hypothetical protein"
                     /protein_id="AED99438.1"
                     /transl_table=11
                     /translation="MRSSRTRSFRKLFTVLPQRIKETSYKNYELWKENPFHPSLEFKKV
                     KPKENIWSVRVGIGWRALGVMKPNEDKIVWFWIGSHAEYDKILGTK"
     gene            complement(8603..8689)
                     /gene="pagE3"
     CDS             complement(8603..8689)
                     /codon_start=1
                     /gene="pagE3"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_precursor"
                     /gene_kind="biosynthetic"
                     /product="cyanobactin precursor peptide"
                     /protein_id="AED99439.1"
                     /sec_met_domain="cyanobactin_precursor (E-value: 1.0,
                     bitscore: 1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKKNLKPQQTAPVQREINTTSSVGGTG"
     CDS             complement(8831..9064)
                     /codon_start=1
                     /note="Orf8"
                     /product="conserved hypothetical protein"
                     /protein_id="AED99440.1"
                     /transl_table=11
                     /translation="MKDEDIDTSDIPPLSEGFWTKAQLRIPKPPVTVEVEVDSETFAWF
                     QAQGETAPKQMSVALKIYVQANKAFSSSTSNI"
     CDS             complement(9075..9362)
                     /codon_start=1
                     /note="Orf9"
                     /product="conserved hypothetical protein"
                     /protein_id="AED99441.1"
                     /transl_table=11
                     /translation="MKFEWDEGKNQTNLIKHGFDFTDAYRIFNLPMVVELDERENYRED
                     RFVAIGLLDGRVVVIVYTEPDDQTIRIISLRKALSYERKYYEQYIKNRLE"
     CDS             9369..9653
                     /codon_start=1
                     /note="Orf10"
                     /product="transposase"
                     /protein_id="AED99442.1"
                     /transl_table=11
                     /translation="MIAKIINYYMRSLIYKGFCQRYKPKEKTPTQCHWGSKLLAGVHLS
                     EKGSTTNPKKSKSRLVQTPCQVSESPELTRIVSQFIEANRAPRETEKDF"
     gene            complement(9616..9852)
                     /gene="pagE2"
     CDS             complement(9616..9852)
                     /codon_start=1
                     /gene="pagE2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_precursor"
                     /gene_kind="biosynthetic"
                     /product="cyanobactin precursor peptide"
                     /protein_id="AED99443.1"
                     /sec_met_domain="cyanobactin_precursor (E-value: 1.0,
                     bitscore: 1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKKNLKPQQAAPVQRENTATVARATVARDGAIAPSSWLSMGRKG
                     SSFCGRRRRVIPLILTLTSKSVKSLFLSPLAHG"
     CDS             10031..10615
                     /codon_start=1
                     /note="Orf11"
                     /product="conserved hypothetical protein"
                     /protein_id="AED99444.1"
                     /transl_table=11
                     /translation="MIAVKGKFPKLTPEEYFIWEEKQLLRHEYLSGRVYAMSGGTQNHG
                     RIASNIIFILKGHLRGGGCQVGNSDCRVNIFETKDYVYPDVSVTCDERDRTAIQAIQYP
                     CLIIEVLSASTANYDRGDKFRMYRRNPSLQDYVLVDAEKIAIDLYRKNDRGNWEIFNYQ
                     SGDNIDLQSIGLIFPIESVYEDIIFEELGTN"
     gene            complement(11007..11162)
                     /gene="pagE1"
     CDS             complement(11007..11162)
                     /codon_start=1
                     /gene="pagE1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_precursor"
                     /gene_kind="biosynthetic"
                     /product="cyanobactin precursor peptide"
                     /protein_id="AED99445.1"
                     /sec_met_domain="cyanobactin_precursor (E-value: 1.0,
                     bitscore: 1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKKNIRPQQSAPVQRQVTTTSCQGEKALFASTECDILTRQCTPF
                     AGDDAE"
     gene            11350..13422
                     /gene="pagG"
     CDS             11350..13422
                     /codon_start=1
                     /gene="pagG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 95.8; E-value: 5.9e-29)"
                     /gene_kind="biosynthetic"
                     /product="C-terminal cyanobactin protease/macrocyclase"
                     /protein_id="AED99446.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 7.1e-24, bitscore:
                     75.1, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 2e-199,
                     bitscore: 653.6, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDLITIPGIPELWTQTKGDSRIKIAILDGAADLERACFKGAKIT
                     QFKPYWAEDIELNDEYYHYLKLATEFNQQQKAKKEDPDHDKEEAKKEREAFFKDFPEDI
                     KRRIDLSSHATHISSTILGQHGSPVEGIAPNCTAINIPISFAGDDFISFVNLTHAINEA
                     LKAEVNIVHIAACHPTQSGMAQEIFARAVKQCQDSNILIVAPGGNDKGECWCIPSILPD
                     VLTVGAMRDDGQPFKFSNYGGEYQHKGVMANGENILGANPGTDEPVREKGTSCAAPIVT
                     GISALLMSMQLQRGEKPNAETVRQAILKSAIPCDQNEVEEPERCLLGKLNIPGAYNLLT
                     GERLTTVKTSEIRQSEITIPVNNITPLTKIENPINSPLLTLSNVIASNSSAVAVANITA
                     SQPTNGITASAASKLVYVLGTIGYDFGSEARRDSFKQLMPPVEIDGITIPANPYDASQI
                     VNYLAENSSESKSLIWTINQEFNPVYALEVKGGFAADVYEMLNLMLAGQIEPESSDDYV
                     ERVSIPGQITDKTITLFSGQEVPVITINNIRGMYGWKVNGLVDAALQTLSEELADANEI
                     QMRRSLSSFLKRVYFDLQNLGKTSKDRALNFAATNAFQAACSFAQAVSTGMELDTIEIE
                     KSPFCRVNSDCWDVKLKFFDPERGLRAKKVYLFSIDVSYMIPVTLGQVRSWSVPK"
ORIGIN
        1 ctaacaccaa attcgacccc gacggaaagg agcatcaggc tcttttcggg ctgcaacttc
       61 ttctttaacc ttttgtgacc aaaaaccata atccgaaagt ccagtcatca tacaatcttg
      121 ttgaatttct tttttttcgg gtttaccaac agatttagtc actttatttt ttgccgttgt
      181 cttcttttct tcgctcataa ttctcttaac tcctagttaa atttaagtta gataaacaac
      241 ctatattaaa atccacatcc acaactacca gaagacttaa gttgttgata gcttctgtga
      301 gcaaaagccg ccggatcatt gtaatttgca ccgtgtaata atttcaatct aatactaagt
      361 aattcttctt ctgttccgtt aaccacatct acagagcgat ggggataaat aacatcaggt
      421 cttttgactg gaggggaaag gttaggtaat ctcatttgat ttaatcctta ttgattattt
      481 atttttaatt ccccccatta gtaagggggg gtaggggggg gttaataagc acttgaccaa
      541 gaacgaactt cacccaaggt gacaggaatt gtatcactaa catcaatagt aaagcgatag
      601 atttttttgg cgcgacggct attttcaggg tcaaagaatt tcagtttaac atcccaacaa
      661 tcactatcta aacgacagaa tggactcttt tctactgtaa tactatctaa ttcataacca
      721 gcacccaccg cttgagcaaa ggtttgagcg gcttggaaag cattagtaga agcaaaattt
      781 aaggctctat cttgagaagt tgtacctaag ttgcgtaaat cataataaat ccgatttaag
      841 aagctgctta aggtgcggcg aattgcatct tcttgagcat caccagcttc tgattgcaca
      901 ctttcaatcg cagcactaac taaagagtta actttccaac cgtacaaacc acgagtatta
      961 ttaatctcaa ttacgggaac aacttgccct gagaataatt taacactccg tccggttaaa
     1021 acaccaggaa tactaactct ttgtacaaat tcagggctat tttcttcttg aatttgtccg
     1081 cttaaaagtc cctgtaacac ttcataaaca tcacgggaaa aaccgcctac gggttcgatc
     1141 gcataaatag gagttaattc taaatttaat gtccaaatta acgctttagc ttcgggtaaa
     1201 ttttccccta aataatccac catttgacga gcatcgtagg gattagcagg aatcattgtt
     1261 ccttcaatac taacacctgg cattaactgt ttaaaggaat ctctacgagc ttccgaaccg
     1321 aaatcataac ccaatacacc taaagcataa actaagtttt tgccttcaag ttcgctaggt
     1381 gcttcactgg cggtaacaaa atttgaagta cggtttgaca tggtaggttg gtttgtagtt
     1441 tgttgaggga atgaaggaat attaatcggg gtttgggtag ttacagaagc aactatttca
     1501 tcaggtttga tttctgatgc tgtttctagt tgattatcca attcagactc gttaatttga
     1561 gtgtcttggc aaccacaacc agaagcttct acgctttcta attctaagtc ttcggacatt
     1621 gtttctcctg ttaagtgttc tatcgcatca aggatgttta attttcccat taaacagcga
     1681 ctttgatcat cggtgtcttt gggattacag ggagtagcac ttgctaaaag ggcatttttg
     1741 actttttgag ggtcaggttt ttcccctctt tttatttgta aactcagtaa taaagcggcc
     1801 actccactca caataggagt cgcaaaactt gtaccactca atctaattgt accaccgttg
     1861 ggttttgctc ctaaaatatc ttttccaggg gcaagaatgc cttgcttttg gtatgcgtct
     1921 ccccagttac taaaatcaac gggtttaccc tggtcatcca ttgctcccac tgctaaaacg
     1981 gtgggcaaag atgcggggac gtgcaaacat tcacagccat catttcccgt tgctgcgatt
     2041 agcaatacgt tgttttcttg gcataattga atggcttttt ctagccaagt atctgcttct
     2101 cctgcatcag ttaattgtcc cgcactaaca ttaataatat tagcaccgtt attaacagct
     2161 tgctctatcg cacgggataa gtctaattgc gataatttca agctttcatc ggcaaaaacg
     2221 ggaacaatta aaccccgaca ttggggagca attcctgtta ctggtgaatc atgttgaccg
     2281 aatataatac tagcaacatg agtcccgtga gttgacattg agccgttggc gttggcttct
     2341 cccgatacta aactagggag tcgggttaag tcagcaccga tgaaacaggg gtgattttgg
     2401 tcaactatac cgtcaagtac agcaacacaa attttaggat cacctctagt ttctgaccat
     2461 agttttttta gtccgggaat gttagtcatt ttggttatct gattgtattt gttttacccc
     2521 cccaaccccc cttactaagg ggggctttaa taaaaaaccc ccacctcctt tagtaagggg
     2581 ggctttaata aaaaaccccc acctccttta gtaaggggga gcttctggaa gatttctcaa
     2641 ggattgaatt ttatctctga tgatttcaca aactgctgaa atattatcat atacgtcatc
     2701 attggtaaac ctcagaaaag taattctagc tgactcgata aactgttgtc tttcttggtc
     2761 gtattcttgc actccttctt gaaaatgact gtcgccatca acttcaatag caagtttaat
     2821 ttctgggcta taaaaatcaa taataaattg agctacacta tattgtcgcc gaaatttaca
     2881 gttttctaat tgctttcctt taagttgtaa ccacaatttt tgttccgcag gagtcatgtt
     2941 attgcgtaaa aattgtcgtt tctctttttc agaatttctg ttataaagtt cggtcatata
     3001 gtttagtcat atttaatcat aattttgttc tttaatacat aaccccccaa cccccattag
     3061 taaggggggc taaaagcttt taatttcccc tgagtaagag tagctaggga tgcttcctct
     3121 tattcccccc tttagtaaga gtagctagag gtgcttcctg cttcctctta tccccccctt
     3181 agtaaggggg gttagggggg tagaaacaga tgggaatgtt agtcattttg ttcatgttga
     3241 tgtttttctt ggttatttaa tttgtaaagg atttgtaata acaaagtcta atatttataa
     3301 actggttgaa ttaaatacct cggagacttt taagtttaat tctttaagtc caggagaggc
     3361 aactaaatca tcgttggtaa aattaccgat ttccctatag gtatctccag ttagttctaa
     3421 gactaaaatg gtttgagttt gaggatcaac aatccagtat tcgggaatgc tacaatcttg
     3481 atattgtaat tttttggcga tgtaatctct ttctctttga atttctccag gactaaccac
     3541 ttcaatgact aataaaggtg gtgacatgga aagaagaatg gtatttctct tggagagtaa
     3601 ttgaatatgt tcttctcgaa taatggtcaa attagggtaa cggtttttcg gttcacctct
     3661 gacttctaat tctaagccat gacctctaac tctatcagtt cctagtatta aagcaaaaac
     3721 caaaaaaagg cgattagcaa tttgaaagtt aagtccagat tctggaggca tttctaccaa
     3781 ttctccatta aataattcac agagtttttc tgaattatcg tcatattgta aatactcagc
     3841 aaaagtttga aagcggggtt tcgtgattaa catcaatttg actccatttt aaatgatttg
     3901 taataataca gtctaatatt ttctattctt gttttttcta attctttttg ggcagtacct
     3961 acccacatat aaggtaatat atcttgatgt tgataaaagc tgtgaactcg ttgagcggta
     4021 tcgttgagtt taaaataatt agttaaatct tgtctatttt taagatgata atagagaact
     4081 ggattattat ttgctttaga caaacccgta aaaaataaac ttgaagcttt cagaggttgt
     4141 aaggctgttt tggggaagtg ttgccatact aagttattaa tttctggttt gaaaaaatca
     4201 tcttcttgca cttcggcata aatttctatt tcacttttac cattaaaata aaaatcaaaa
     4261 ccaatcaagt tgacaaaatc tttcaggtaa ttaccatccg ccgcaccatc acttaaggca
     4321 aaagctttat ccaacttttc gggataatct ttgattctaa tgtgcatttt taagctagat
     4381 tcagccagat tgtttcttaa gtcaatcccc gcagaaagaa cggtaacttt actaaaatca
     4441 aaactagatc ctaaaaattg ctgtaacaaa gaatagttga ttttcacacc aacacggttt
     4501 tctaccaggc caaaaaatgt caaagattga tggagatatt tttgccattc ttgtgtttta
     4561 tcttcaaaaa aaagcagaaa tcttgaggca atcaatttgt ctgattctat tttacaagat
     4621 gcttcaaggc tacagtcacc ttcaatgctc gttacaaagt cttcaaataa ggggagcgga
     4681 taaattggct caacgtcaaa agcttgttga tgattgcgaa taaactgtag tttttgttct
     4741 ttgagtctgt ctttttggat tacattaact atcataaatg gctagttttt atcttgttaa
     4801 aaagtatgcc tagaaaacct cgaaatttac agtctggata ttcttaccat attaccatcc
     4861 gttgcaataa ccgtgagttt aaactgcaaa agcgtgagtg tcgggaagtg tttctttacg
     4921 cgatcaagaa ggctttgaac aaatacaatt tcaagcttta tgctctctgt atcatgtcta
     4981 atcatgtcca ttacctgatt gaacctgccc aacccgaaga tttacctaaa attatgcact
     5041 ttctcaattg gtacacggcg atgtgtttca accgtatgtt aaggagaaca ggacactttt
     5101 gggaaaaacg ctattttagt gatggctttc ctgcttcaga caaggacagg gctttaaata
     5161 ccctgcgcta cattcacggc aacccgaaag cggcaaaaat gcgttctggt ttcttctacg
     5221 atttcagtaa ctacggcagt tatgaacgtt taaccgatga cggtttaact caatggcatc
     5281 ctgctttcct acaactaggg aatagtttag aagactgtgc caagaaatat aaaggctttt
     5341 gccaaaggta caaaccgaag gagaagaaga ccccaacccg atgccattgg ggtagtaagc
     5401 tgttggctgg ggttcatcta tcagaaaaag gctctaccac caacccccaa aaatctaagt
     5461 ctgtgcttac aagctattac caaccactca cccccaaaaa atctaagtct cgtcttgttc
     5521 aaaccccgtg ccaggtatcc gaaagccccg aactgactag gattgtgagt caattcatag
     5581 aggctaaccg tgcgccaagg gagacagaaa aagacttttg acactttttg aggtgagtgt
     5641 tagaatcaaa gggattactc cgcatcgtcg cccgcaaaag gtaaagggat tcccaagtat
     5701 gcctgatggg gtgtcagacc tgtacttgta ccactctcag aggaagtggt attgatttcc
     5761 cgttgcacag gagcggcttg ttggggtttg aggtttttct tagtcatggt ttgtttctct
     5821 agaatttaag gtgaaattga cttgatgagc attaaccaaa tagctcagga cttgtcaaat
     5881 acttttgtgg gaattcttga ctaaatgctt cctgtaatta atattaagta atattgctac
     5941 agaggaagtg gtattacttc ccgaccgtgc caggtatggg tcaagccccg aactgactag
     6001 gattgtgagt caattcatag aggcgaaccg tgcgccttgg cagttggaaa aagacttttg
     6061 acactttttg aggtgagtgt tagaatcaaa gggattactc cgcatcgtcg cccgcaaaag
     6121 ggtacaaata agggttaata tggggtgtca gacctgtact tgtaccactc tcagaggaag
     6181 tggtattgat ttcccgttgt acaggagcgg cttgttgggg tttgaggttt ttcttagtca
     6241 tggtttgttt ctccagaatt taaggtgaaa tttgacttga tgtcaatata caggtgtgac
     6301 taccctcttg tcaatagtct tctagcattg gtgagttaag ggaaagggga acccaaaaga
     6361 aaaacaacct ctactcctcc tcccccctac tcctcccaag gggactactc cgcgtcgtcg
     6421 cccgcaaaag gatgcctgaa atcccctata ctccgaaggg gtgtcagccc tgtaccagcc
     6481 tcagaggaag tggtattgat ttcccgttgc acaggagcgg cttgttgggg tttgaggttt
     6541 ttcttagtca tggtttgttt ctccagaatt taaggtgaaa tttgacttga tgggtatcgt
     6601 aaccaaatag ctcaagactt gtcaatattt ttgtgagatt tcttgactaa atgcttcctg
     6661 taattaatat taagtaatat tgcttacaac ctatcattaa ctactcaccc caaaaaatct
     6721 aaatctcgtt tcacttctct accgatgccc actatccgaa agccccgaat taacaaggat
     6781 tgtgagtcaa ttcattgaag ctaatcgtgc gccaaggcag gcagaaaaag acttttaata
     6841 ctttttgagg cgaatgttag aatcgaaact aaaagggtca taccaactaa acaaaccagc
     6901 actatcatcc cttattttat ttatactgaa ccctatttgg ggtgtcagcc ctgtaccacc
     6961 cacagaggaa gtggtattga tttcccgttg cacaggagcg gcttgttggg gtttgaggtt
     7021 tttcttagtc atggtttgtt tctccagaat ttaaggtgaa atttgacttg acgataattt
     7081 ttaacataat atgatggcaa tatggcatcc cgcttttcta acaattaggg aacagtttag
     7141 aagaatgtgc cagaaaatac aaaggctttt gcttacaagc tatcatcata aaggataaaa
     7201 ggagattcat gatgaaaaaa gtaattcaat ctactggaaa gatagatagg gtgggtcaat
     7261 tatctttaga ctatccaatt aaagggactc cacccagttc ggtaagagtt attattcttt
     7321 gggaagaaac agaaacagag gttaatgact tttgggaaca aatcaaagaa tatcaacaat
     7381 atcctttaat gtcagcagaa caattacaac tggaattaaa acaatctttg accgaagcag
     7441 gttatgattc aagggagaaa attgttgatc ttgtccaaga tattaaacgg gaaatatctc
     7501 aagaacgtca acaaaaacaa gattcaatac aatgacgaaa attcctgaat taattttttt
     7561 agataccaat gtttatttga ttggcgcagt tgagcgacaa agccctgaag gtttaattct
     7621 aaaatggtta ggttgggaag ccccaaacga taattctgtg ggggtaataa tttctgaaga
     7681 acttattgat caaattgcca gagttgctaa tttacaaagt attggtttaa tctttcccat
     7741 tgagtccgtt tatgaggata ttatttttga ggaattagga acaaactagg atttctaaaa
     7801 cttgttcttt aattatttct atctctcgaa taagactcca atctcacccc caaaaaattt
     7861 aagtctcgtt taacttcccc accgtgccag gtatccgaaa gccctataag gattgtccgt
     7921 caattcgttg aggctaaccg taccccatgg caagtagaaa aagagttttg acattttttg
     7981 aggtgagtgc taaaattagg gggactactc ccccgtcatc gcccgcaaaa gggtgttgcc
     8041 agattggttt tgcttttgtt tgttttcaga aactcaggaa gaatctatga ctcatttact
     8101 agaaaaagca ttccaagaag ctcaaaaact ctcaaattat cttcaggatg aacttgcaca
     8161 acaattactc gaagatatcg agaatgagtt aaaatggcaa gaaagtttat ctaatccaga
     8221 tatggattta ggtatttttc aagaaatggc tcagttagca ttacttgaag atgaagaagg
     8281 taagactgaa gataagggtt ttggtgagaa ataatgcgat catcaagaac tagaagtttt
     8341 agaaaattat ttacagtatt acctcaaaga atcaaagaaa cttcctataa aaattatgag
     8401 ctttggaaag aaaatccatt tcatccgagt ttagagttta agaaagtcaa acccaaagaa
     8461 aatatttggt cagttagagt aggaatcgga tggagagcat tgggagtaat gaaacccaat
     8521 gaagacaaga tagtttggtt ttggattggt tctcatgctg agtacgataa aattcttggt
     8581 acgaaataat agttgtttaa tttcaccctg taccacccac agaggaagtg gtattgattt
     8641 cccgttgtac gggtgcggtt tgttggggtt tgaggttttt cttagtcatg gtttgtgtgt
     8701 ctccacattt agtttaagtt attttgggag catcctaaat taaagctagg ctatttgtca
     8761 agatttttgt tattttgttc acccatttta ctttgactac ataccccctc ttaattcccc
     8821 tttagtaagg ctaaatatta cttgtagatg aagaaaaagc cttatttgct tggacataaa
     8881 ttttcaacgc tacagacatt tgttttggag cagtttctcc ttgtgcttga aaccaggcaa
     8941 aagtttctga atctacttca acttctacag taacaggagg cttagggatt cgcaattgtg
     9001 ctttagtcca aaaaccttct gacaggggag gaatatcaga agtatcaata tcctcatctt
     9061 tcatcgcatc aattctattc caatctgttt ttgatatatt gctcataata ttttcgttca
     9121 taagataatg cttttcttaa agatataata cgaatcgttt gatcatcagg ttcagtatag
     9181 acaataacca caactcgtcc atctagtaaa ccaatagcaa caaagcgatc ttctctataa
     9241 ttctctcgct catccaattc aacaaccatt ggtaggttaa agatacgata agcatctgta
     9301 aagtcaaatc catgtttgat aaggttagtt tggtttttgc cctcatccca ttcaaatttc
     9361 atagatttat gatagcaaaa ataatcaatt attatatgcg atcgcttata tacaaaggtt
     9421 tttgccaaag gtacaaaccg aaggagaaaa ccccaactca atgccactgg ggtagtaagc
     9481 tgttggctgg ggttcatcta tcagaaaaag gctctaccac caaccccaaa aaatctaagt
     9541 ctcgtcttgt tcaaaccccg tgccaggtat ccgaaagccc cgaattaaca aggattgtga
     9601 gtcaattcat tgaagctaac cgtgcgccaa gggagacaga aaaagacttt tgacactttt
     9661 tgaggtgagt gttagaatca aggggattac tctgcgtcgt cgcccgcaaa aggaacttcc
     9721 ctttctaccc atactgagcc acgaagaagg tgcgatcgca ccatcacgag ccacagttgc
     9781 gcgagccaca gttgcggtat tttcccgttg tacgggtgcg gcttgttggg gtttgaggtt
     9841 tttcttagtc atggtttgtg tgtctccaca tttagtttaa gttattttgg gagcatccta
     9901 aattaaagct aggctatttg tcaatatttg taataattat taactatttt tagtgagatt
     9961 gtgtttgggt ggagtgttag aatagactag atgattgcca attgctgaga gattactaaa
    10021 ggtaggtaaa atgattgctg taaaaggtaa gtttcctaag ttaacacccg aagaatattt
    10081 tatttgggaa gaaaaacaac tgctgcgcca tgagtatctt agtggtagag tttatgccat
    10141 gagtggaggg actcaaaatc atgggcgaat tgctagtaat attattttca tccttaaagg
    10201 tcatttacgg ggcggtggtt gtcaggtagg taattccgat tgtcgtgtaa atatttttga
    10261 aactaaagat tatgtttatc ctgatgtgag cgttacttgt gatgagcgcg atcgcactgc
    10321 aattcaggcg attcaatatc cttgtctaat tattgaggtt ttatctgcaa gtacagccaa
    10381 ttatgaccga ggggataaat ttaggatgta tcgtcgcaat cctagtttgc aagattatgt
    10441 tttagttgat gccgagaaaa tagcgatcga tttataccgc aaaaatgatc gtggtaattg
    10501 ggaaattttt aattatcaat cgggggataa tattgattta caaagtattg gtttaatctt
    10561 tcccattgag tccgtttatg aggatattat ttttgaggaa ttaggaacaa actaggattt
    10621 ctaaaacttg ttctttaatt atttctatct ctcgaataag actccaatct cacccccaaa
    10681 aaatttaagt ctcgtttaac ttccccaccg tgccaggtat ccgaaagccc caagaagcac
    10741 tagaaaacgc gaaggaagtt ttagaagatt tagtgtatgg ttatgagcaa atagggaaaa
    10801 ttttgccaga accaaagaca ttacaggtcg cttgaaaccg attagtaatt tatgttgctc
    10861 cttattcccc ccttagtaat agtggctaaa gttgcttatt attccccctt actaaagatg
    10921 attaaaagtg ctgtgcttat tattcccccc ttactaaggg gggctagggg ggtagaaact
    10981 caggtgagtg ctaaggatta aagggactac tccgcgtcgt ctccagcaaa gggagtacat
    11041 tgtctggtta aaatatcaca ctctgtacta gcaaataatg ctttttcacc ttgacaagaa
    11101 gttgttgtca cttgacgttg taccggggca gattgttggg gacggatatt tttcttgatc
    11161 attgtttgtg tctccagatt agttgaagtt tgactgactg gatgataatc taaggttatg
    11221 ctggtggttg agtctttgta atgattatta acaatgctta ttagcaaata ggtaaagttt
    11281 ctctgtacaa tgctcaagag tagagtgctt ctaatgtaaa ctaaaattat ttgctcgata
    11341 actataacta tgccagattt aattactatt cctggaatac ctgaactttg gactcagacc
    11401 aaaggcgatt ctcggattaa aattgctatt cttgacggtg cagcagattt agaacgtgct
    11461 tgttttaaag gtgcaaagat tactcaattt aaaccctatt gggcagaaga tattgaactc
    11521 aatgatgagt attatcatta tttaaaatta gcgacagaat ttaatcaaca acaaaaggct
    11581 aaaaaagaag acccagatca cgataaagaa gaagcaaaaa aagaaagaga agcctttttt
    11641 aaagatttcc ctgaagacat taaaagacgg attgatttat ctagtcatgc tactcatatt
    11701 tcgagtacaa ttttaggaca gcatggctct cccgtggagg gtattgcgcc taattgtacg
    11761 gcgattaata ttccgatttc cttcgcagga gatgatttta tttcctttgt taatctcacc
    11821 catgcgatta atgaagcatt aaaagccgaa gttaatattg ttcatattgc ggcttgtcat
    11881 cctactcaat caggaatggc tcaagaaatc tttgctcgtg ctgtgaaaca atgccaagat
    11941 agtaatattt taattgtggc tccgggtggt aatgataaag gtgaatgttg gtgtattcct
    12001 tcgattttac ccgatgtttt aacagtgggt gcaatgcgtg atgatggaca gccttttaaa
    12061 tttagtaatt atggtggtga atatcaacat aaaggcgtaa tggctaacgg tgaaaatatc
    12121 ttaggtgcga atcctggtac cgatgaacct gttagagaaa aaggaacaag ttgtgccgct
    12181 cctattgtta ctggaatttc agctttatta atgagtatgc aactgcaaag aggggaaaaa
    12241 cctaatgctg aaaccgtgcg acaggctatt ttaaaaagtg cgattccttg cgaccagaat
    12301 gaagtagaag aaccggaacg ttgtttatta ggaaaactca atattccggg ggcttataac
    12361 ttattaacag gagaacgatt aacaacggtt aaaacttctg aaatcagaca atcagaaatt
    12421 actattcctg ttaataatat tactcctctc actaaaatag aaaacccaat taacagccca
    12481 ttactaactt taagtaatgt aattgccagc aattcttcgg ccgtagctgt ggctaatatt
    12541 accgctagtc aacctactaa tggtattact gctagtgctg catccaaatt agtttatgtt
    12601 ttaggaacaa ttggttatga cttcgggagt gaagcgagac gagacagttt taaacaatta
    12661 atgccacccg tagaaattga cggaataacc attccagcta atccctatga tgcttctcaa
    12721 atagttaatt atttagcaga aaattcttca gaaagtaagt ccttaatttg gacaattaac
    12781 caagaattta acccagttta tgccctggaa gtaaaaggag gatttgcggc tgatgtttat
    12841 gaaatgttaa acttaatgtt agccggacaa attgaacccg aaagcagtga tgattatgtt
    12901 gaaagggtga gtattcctgg gcaaattact gataaaacaa ttaccctatt ttccggtcaa
    12961 gaagttcctg taattacgat taataatatt aggggaatgt acggctggaa agtaaacggt
    13021 ttagtggatg cagccttaca aaccctttct gaagaactag ctgacgctaa cgagattcaa
    13081 atgcgaagaa gtttaagtag tttcctgaaa agagtctatt ttgatttgca aaatttagga
    13141 aaaacctcta aagaccgggc gttaaatttt gcagcaacta acgcttttca agccgcttgt
    13201 agttttgctc aagctgtttc taccgggatg gaattagata caattgaaat cgaaaaaagt
    13261 cccttctgtc gggttaatag tgattgttgg gatgtgaaat taaagttttt tgatcctgaa
    13321 cggggtttga gagccaaaaa ggtttatctt tttagtattg atgtgagtta tatgattcca
    13381 gtgacattgg ggcaagttcg ttcttggtct gtacctaagt ag
//
