LOCUS       BGC0000480             11821 bp    DNA     linear   BCT 20-APR-2008
DEFINITION  Nostoc spongiaeforme var. tenue str. Carmeli TenA (tenA), TenB
            (tenB), TenC (tenC), TenD (tenD), TenE (tenE), and TenF (tenF)
            genes, complete cds; and TenG (tenG) gene, partial cds.
ACCESSION   BGC0000480
VERSION     BGC0000480
KEYWORDS    .
SOURCE      Nostoc spongiaeforme var. tenue str. Carmeli
  ORGANISM  Nostoc spongiaeforme var. tenue str. Carmeli
            Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2022-11-07 19:38:40
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 11821
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..1992
                     /gene="tenA"
     CDS             1..1992
                     /codon_start=1
                     /gene="tenA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 179.5; E-value: 2.3e-54)"
                     /gene_kind="biosynthetic"
                     /note="subtilisin-like protease"
                     /product="TenA"
                     /protein_id="ACA04480.1"
                     /sec_met_domain="cyanobactin_synth (E-value: 1.9e-235,
                     bitscore: 770.5, seeds: 40, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S8 (E-value: 8e-39, bitscore:
                     122.5, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLETLSTISQGNNSICVAVLDGPVDRTHPCFQGADLKYLPSLVKD
                     DAKINGNMSAHGTHVGSIIFGQPGSLVQGIAPQCKGIIVPVFSDDRLRTSQLDLARGIE
                     QAVNAGAHIINLSGGQLTDYGESDGWLQNAVRLCRENNVLLVAAAGNNGCDCLHVPAAM
                     PAVLAVGAMDANGKPLDFSNWGEAYQSQGILAPGENILGAKVGGGTHRLSGTSFAAPIV
                     SGVAALLLSLQLERGETPDPHKIRQVLLQSALPCDADLPEDAKRCLAGKLNVSGAITLL
                     KGGKMAEEFTSVSASEVEAAGCGCGSSLTSASEALAAHQGQPAPSLVGAAAKVEGAQSV
                     PSNTPNFQNYIQSFPTPVTQPMMPNLTANSVTASQAPSELPDLGPLVYSLGTLGYDFGT
                     EARRDSFKQLMPPFDIGGGVMVPANPYDARQMVDYLDANISEARSLIWTLNIELTPVYA
                     IDPTGPFAAESYRALHELLSGQIQAESDAEYVERVSIPGILTKRTVKLYSGQVIPVLEP
                     QSTRGIYGWKVNNLVSAAMAAVQAEAGAADEETIRKTLDGFLNRIYYDLRNLGMTSQDR
                     ALNFAVTNAFQAAQTFSEAVAVGMELDSVTVEKSPFCRMDSDCWDVKLKFFDPENNRRA
                     KKIFRFTIDVSDLIPVTLGEVRSWSSPY"
     source          1..11821
                     /country="Israel: Bet Dagan"
                     /db_xref="taxon:503013"
                     /mol_type="genomic DNA"
                     /organism="Nostoc spongiaeforme var. tenue str. Carmeli"
                     /strain="Carmeli"
                     /variety="tenue"
     subregion       1..11821
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="RiPP"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11821
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            2088..2336
                     /gene="tenB"
     CDS             2088..2336
                     /codon_start=1
                     /gene="tenB"
                     /product="TenB"
                     /protein_id="ACA04481.1"
                     /transl_table=11
                     /translation="MRLPILSPPVKRPDVIQPALCVDLENGSPDDLVHIRMDLLHGANY
                     NDPPAFANRSFNQVMHSSASQGMSQGWGGFGSMGGFY"
     gene            2363..3088
                     /gene="tenC"
     CDS             2363..3088
                     /codon_start=1
                     /gene="tenC"
                     /product="TenC"
                     /protein_id="ACA04482.1"
                     /transl_table=11
                     /translation="MPQQTQTLWQIGQPGKGGQEFSQEGGWQGEFTYVVGSDSDPINQP
                     QMPSLLVVPGLKKKPRSKGKQLFSTEKLNIQFTLTQSYNPGELTLFYNFFGSEVDTLWV
                     DGEPVTEIVGVGSSKVTQAQIPLPALSAGEHTLTLTTASQSGDGAHRIDYLKLEAVVAT
                     QQPQTLQTPQTPKETMAKEKTETTPPAVSPTPEPKKKKSYVPTSQTGLQDYSYWWEYAR
                     QHAKAPEEGKSFRRGRIWA"
     gene            3499..5832
                     /gene="tenD"
     CDS             3499..5832
                     /codon_start=1
                     /gene="tenD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Goadsporin_CD_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Cyanobactin_D_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: NHLP_CD_RRE"
                     /gene_kind="biosynthetic"
                     /note="Putative heterocyclizing protein"
                     /product="TenD"
                     /protein_id="ACA04483.1"
                     /sec_met_domain="TIGR03604 (E-value: 4.4e-79, bitscore:
                     254.8, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 2.8e-92, bitscore: 298.8,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="Cyanobactin_D_RRE (E-value: 3.3e-43,
                     bitscore: 134.7, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="Goadsporin_CD_RRE (E-value: 8.7e-21,
                     bitscore: 62.9, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="NHLP_CD_RRE (E-value: 1.6e-23, bitscore:
                     71.6, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQSTTLLQIKPHFHIEVIEPKQVYLLGEQGNHALTGELYCQIVPL
                     LDGQHTIEQIIQKLDGQVPAEYIDYVLNRLAEKGYLTEATPDLSPEVAAFWTELGIAPT
                     VAAQGLKQPVTLTTVGENISEVTVAALATALRDMGIPVQNASDIGSSAALNIVLTDDYL
                     QPELAAINKQALQSQQTWLLVKPVGSVLWLGPVFVPQKTGCWSCLAHRLRGNREVESSV
                     LRQKQAQQERNGQQGRVVSSLPTARATLPSTLQTALQFAATEIAKWIVKQYVNATAPGT
                     ALFPTLDGKVITFNQTILDLKSHLLIKRPQCPTCGDPEIMQRRGFEPLKLESRRKRFTH
                     DGGHRATTPEQTLQKYQHLIGPVTGVVTELVRITDPANPLVHTYRAGHSFGSATSLRGL
                     RNTLRHKSSGKGKTDSQSRASGFCEAVERYSGIFQGDEPRKRATFAELADLAIHPAQCL
                     HFSDEQYTNREALNAQGTEAAYRWIPHRFDASQAIDWTPVWSLTEQRHKYLPTGLCYYH
                     YPMPEANRFCKADSNGNAAGNTLEEAILQGFMELVERDSVALWWYNRLSRPGVDLTSFN
                     EPYFVQLQQFYREQNRELWVLDLTADFGIPAFVGVSYRTVGTSERIIVGFGAHLDPTIG
                     ILRTLTEVSQIGLELDKIPDEQLKDESKDWLLGVTRESHPCLVPDPSQPLKTANDYPKR
                     WSDDIYTDVMTCVKIAQGIGLETLVLDQTRPDIGLNVVKVIIPGTRGLWSRFGPGRLYD
                     VPVKLGWRTVPLVEAEMNPMNIPF"
     gene            5968..6249
                     /gene="tenE"
     CDS             5968..6249
                     /codon_start=1
                     /gene="tenE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: TIGR03678"
                     /gene_kind="biosynthetic"
                     /note="tenuecyclamides A and C precursor protein"
                     /product="TenE"
                     /protein_id="ACA04484.1"
                     /sec_met_domain="TIGR03678 (E-value: 3.6e-25, bitscore:
                     77.0, seeds: 4, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKNILPQQGKPVIRITTGQLPSFLAELSEEALGDAGVGASATG
                     CMCAYDGAGASATGCMCAYDGAGASATACACAYDGAGASATACACAYE"
     gene            6627..7589
                     /gene="tenF"
     CDS             6627..7589
                     /codon_start=1
                     /gene="tenF"
                     /product="TenF"
                     /protein_id="ACA04485.1"
                     /transl_table=11
                     /translation="MTLTSMLQNNRLKDRRLQFIRTHQEAFDVEPTFILSLFEEAVLGI
                     EGTCGVELMCHVEGDQLFAVDFQVSNERHTWPRSLTDAVKFLDKVESQVGVRLNRDLLQ
                     QFVAVHIGSSKILNNTIGIDLRPRHENSCIKVYMHIEHEEDPEELVRTALKLDGDSYSS
                     EMLQVLLKSTIIIGFNIFFNGYSDVELLVATVGDKYESHKFNRGKYLKHYIQKNFSLKA
                     NYMLRESNILLVSFSQAKKVNPLLIFHYEDIKDIRKYFSFNSLGDRSYSFFQSQDCITY
                     AGVSVRELELQKDRLEKFSLFYNKRDKCQHLPLFSTLNE"
     gene            7919..>11821
                     /gene="tenG"
     CDS             7919..>11821
                     /codon_start=1
                     /gene="tenG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 78.2; E-value: 1.2e-23)"
                     /gene_kind="biosynthetic"
                     /note="thiazoline oxidase/subtilisin-like protease"
                     /product="TenG"
                     /protein_id="ACA04486.1"
                     /sec_met_domain="cyanobactin_synth (E-value: 4.2e-191,
                     bitscore: 624.4, seeds: 40, tool: rule-based-clusters)"
                     /sec_met_domain="PF00881 (E-value: 5.4e-13, bitscore: 38.3,
                     seeds: 78, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S8 (E-value: 3.6e-22, bitscore:
                     67.8, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNHPFAVSLNPFVQIKSTEDYYTLQSVESDSTALLTLATTPGIG
                     IALDHLKAGNTTTESLVQSLTELYGQGTGEQFALMLQQLDERGWLNYAVLPLAEAIPMV
                     DSAELNLTEPYSTQTSLSLSRFAYQHSYDGNMVLESPLSKFRIKLLDWRASALLAQLAQ
                     PQTLGSLTPPPYLGPETAYQFLNLLWAAGFLTADPEPPSLQLWDFHNLLFHSRSRNGRH
                     DYPVMDDNIEQWSNFPVVKPPMSDRIVPLPRPNLGALMCNDATLTQAIEVRKSVREYDD
                     NHPISIEQVGELLYRTARVKKLVRLEDVVGENLHRKHENLKFGHDYGELSRRPYPGGGV
                     MYELEIYPVVVHCQGLNKGIYHYDPLNHQLEQILESEDEIVALLGYEHITKFFISPRPQ
                     VLLVFTARFGRLFRRYRSMAYAAVLKHVGVLQHNFYLVATSMGLAPCALGVGDSDAFGR
                     AIGLDYVEESAVGEFLIGSLPNRNVEASGLESIEVDSSEGLTQTIISVNDIELSEIEAE
                     PEPSETEVLGSQLESVGLVAGIQPTEVCESPENTSAQVEDRGTEAADLAASETMVENAN
                     SSTLAESESDAVAVSVTEVESSASQISLALHPHDLDDRIPGLAQLHNQTLGDPRITIVI
                     IDGDPDHTLSCFARAEVSKVFPYWHEPAEPISPEHYASFQAIRDKGLKGKEKEQAIDAA
                     LPKNVKTRIEINDHACHITSIIVGQEHSPVFGIAPKCRVINMPHDALANHDDIQSPLEN
                     YDDIISPLNLARAFELALELGANIIHCAFCRPTRTSVGEEILVKAIKKCLDNNILIVAP
                     VGNNSNKNWCLPAVLPGILAVGAAKVDGTPAHFSNWGGNNTQEGILAPGVDVLGAQPCT
                     EKPVRQTGSSMAAPVITGISALLMSLQLQQRKPVDAEAIRAALLNTAIPCDPKVVEEPE
                     QCLRGFVNIPGAMKMLFGQPSVTFSFAGDGMTRTERPGFAMPSAMATPASITAASPAVQ
                     PTDATTSETSVTAAADAADPGSVEASIAYDGNVYALGILGYDFGDETRRDSFKELMPPT
                     QVNGIMVPSDPYDVRQMVHYLDRNPDESRSLIWTLSLDGDTIYALDPTGPFSGQIYEIF
                     LLMLAGQLEPETSDEFVERISVPARRTNRTVELFSGEVVPVVNVHDLRGLYGWKVNALV
                     DAAMAAISRRVDEAQAPLVRQALITFLNRVYNDLRNVGQTSRDRALNFAATNIFQAASV
                     FAKAIAERRQFDTIKVEKSPFCRINSDCWDVKLEFYDPENSRRGRKVFRFTLDVRLLMP
                     VTLGEVKSWSLPSNSTD"
ORIGIN
        1 atgctagaga cattaagtac aatttcccag ggcaataata gtatttgtgt tgcggttttg
       61 gatgggcctg ttgaccgcac tcatccttgc ttccaaggcg ctgaccttaa gtacctgccc
      121 agcttggtta aagatgatgc caaaatcaat ggcaatatgt ccgcccacgg cacccacgta
      181 gggagcatta tctttgggca accgggtagt ctggtgcagg gaatcgctcc ccaatgcaag
      241 ggaataattg taccagtctt ttccgacgat cgccttagga cttcccagct agatttagct
      301 agggggattg agcaagcagt taacgccggc gctcacatca tcaacctcag tggtggtcaa
      361 ctaaccgact atggggaatc cgatggttgg ctacagaatg ccgttcgtct gtgccgagag
      421 aacaatgttt tgctcgtcgc cgcagcaggc aacaatggtt gcgattgctt acacgtacct
      481 gctgccatgc ccgcggtact ggctgtgggg gcgatggacg caaatggtaa acccctggat
      541 ttcagcaact ggggcgaagc ctaccaaagc caaggtatcc ttgcccctgg ggagaacatt
      601 ctgggagcaa aagtcggtgg tggtactcat cgcctcagtg gtacgagctt tgccgctccc
      661 attgtctcag gtgtagcagc actgctgctg agtcttcaac tggagcgagg agaaaccccc
      721 gacccccata aaattcgtca agtgttattg cagagtgctt taccctgcga cgcggacttg
      781 ccagaagatg ccaagcggtg tctggcgggc aaactgaatg tttctggagc cataacgtta
      841 ttaaaaggag gaaagatggc tgaggaattc acatcagtca gtgcatcaga agtagaagcc
      901 gcaggctgcg gttgtggtag tagcttaact agcgccagtg aagcgcttgc cgcccaccaa
      961 gggcaacctg ctcctagctt ggttggtgcg gcagccaagg ttgaaggggc acagagtgtc
     1021 cctagcaaca cccccaactt ccaaaattat attcaatcat ttcctacacc tgtaactcaa
     1081 ccaatgatgc ccaacttaac tgctaactcc gtcaccgcca gccaagcacc cagtgaactt
     1141 ccggatcttg gccctctggt atattcacta ggaaccttgg gctatgactt cgggactgaa
     1201 gcccgtcgag actcgtttaa acaattgatg ccaccatttg atatcggtgg cggggtgatg
     1261 gtaccagcta acccctacga tgctcgccag atggtggact acctggacgc caatatttcg
     1321 gaagcgcgat cgctaatttg gacacttaat atagaattaa ctccagtgta tgcgatcgac
     1381 cccaccggtc ccttcgccgc cgagtcatac cgcgccctcc acgaactgct atccggtcaa
     1441 atccaagctg aaagcgatgc tgaatacgtt gagcgggtca gcataccagg catcttgacc
     1501 aagcgcacgg tcaagcttta ctccggtcaa gttatcccag tgcttgaacc ccaaagcact
     1561 cgcggtatct acggatggaa agtcaacaac ttggtgagtg cggcgatggc agcagtgcaa
     1621 gcagaagccg gggctgctga cgaagaaacg atccgcaaga ccttagatgg tttcctcaac
     1681 cgcatctact acgacctccg taacttggga atgacatccc aagaccgtgc cctcaacttc
     1741 gcagtcacca acgccttcca agccgcacaa accttcagcg aagcagttgc agttggtatg
     1801 gaacttgata gcgttaccgt tgagaaaagt cctttctgcc gtatggatag cgactgttgg
     1861 gatgtgaaac tcaagttctt cgaccctgaa aacaaccgcc gtgccaagaa gattttccgc
     1921 ttcaccattg atgtcagtga cttgattcct gttaccttgg gcgaagttcg ttcctggtct
     1981 tcaccgtatt aaaggggaat acaattttga taagagtgca acaacaaaat gcactcttgc
     2041 taagaaatct aaatttgttc accctagcat acggagaatt ttaaatcatg cgacttccta
     2101 tattgtctcc ccctgtcaaa cgtcccgatg taatccagcc tgcgctgtgc gtggatttgg
     2161 aaaatggtag tcctgatgat ttggtgcata ttcggatgga tctgctgcat ggtgccaact
     2221 ataacgatcc tccagccttt gccaaccgta gtttcaacca agtgatgcac tctagtgcca
     2281 gccagggtat gagtcagggt tggggcggct ttggcagcat gggaggattc tattaacgag
     2341 cgactaatag ccgctaatca ccatgcctca acaaactcaa accctatggc aaattggtca
     2401 acccggtaaa ggaggtcagg agttttccca ggaaggcggc tggcaaggcg aatttaccta
     2461 cgtagttggg tcagattcag accccattaa ccagccgcaa atgccatcgt tactcgtggt
     2521 tcctggtctc aagaagaaac cacgcagcaa aggaaagcag cttttttcca ccgaaaaact
     2581 gaatattcag ttcaccctta ctcagagcta caacccaggc gaacttacgc tcttttacaa
     2641 cttttttggc tctgaagttg acaccctttg ggttgatggt gagccagtga ctgaaatcgt
     2701 cggcgtcggg tcaagcaagg taacccaagc ccagattccc ttgccagcct tgagcgctgg
     2761 agaacatacc ctgaccttga ccaccgctag tcagtctgga gatggcgcac accgcatcga
     2821 ctatctcaaa ctagaggcag ttgtcgctac ccagcaaccg caaactctcc aaacaccgca
     2881 aacacctaag gaaactatgg ctaaggagaa aacagaaaca acacccccgg cagtctcacc
     2941 tacccccgaa ccgaaaaaga aaaagtctta cgtgcccaca tcccaaactg ggttacaaga
     3001 ttattcttac tggtgggaat atgccaggca acatgccaaa gcaccggagg agggcaagtc
     3061 cttcagacgt ggccgcatct gggcttagag agtgtataca caagtagaat taccccccct
     3121 taatccctcc ttataaaggg gggaaaccgg aaaatctagc agatgtaggt tgggttgagg
     3181 aacgaaaccc aacacatcaa agtcttacct ttgttgtgtt tcactccgta cctctacggg
     3241 gaaaagctac aatccaacct acgtatctgg ttaatttttt tctggaattg ctattaatcc
     3301 ctaaccaata atttctctca gggtagaaga aagcggataa attctgggac tcaatccgca
     3361 atttaaaatc ctgtgagaag atacccccgg ataaattctg ggggctaatc taaaattcac
     3421 taacggataa ccccgtataa accctgtggg ttaatccaaa atctaaaatc caaaatccaa
     3481 aattgtctga tttgctctat gcaatccacc acactcctcc aaattaagcc tcactttcat
     3541 atagaggtaa tcgagccaaa gcaagtgtat ctgcttggcg aacagggtaa ccatgcccta
     3601 accggggaac tgtactgtca aatcgtaccc ctcctggatg ggcaacacac catcgagcag
     3661 attatacaaa agcttgatgg gcaagtcccg gcggaataca ttgactatgt actcaatcgc
     3721 ttggcagaaa agggctacct caccgaagct accccagatt tatctccaga agtggcggca
     3781 ttttggactg agttaggaat tgcacccacc gttgcagcgc aaggactaaa gcagccagtg
     3841 acactgacaa cggtaggcga gaacatcagt gaggtaactg ttgctgctct ggcaactgcc
     3901 ttgcgggata tgggcattcc agtgcaaaac gcctcagaca taggttcttc agcggcgctc
     3961 aacatcgtct tgaccgatga ctatttgcag ccagaactag ccgccatcaa caagcaggct
     4021 cttcaaagcc aacaaacttg gctgctcgtc aaaccagtag gtagtgtcct gtggttgggg
     4081 ccagtgtttg ttccccaaaa gacagggtgt tggagctgcc ttgcccatcg gctgcggggc
     4141 aaccgcgaag ttgagtcgtc agttttgcga caaaaacaag cccagcaaga gcgcaatggg
     4201 cagcaagggc gtgtggtgag tagcttgcca acggcacgag cgacgctgcc atctacccta
     4261 caaactgcat tacaattcgc cgccaccgag atcgccaagt ggattgtcaa gcaatatgtc
     4321 aatgccactg caccgggaac ggcacttttc cccaccctcg acggcaaggt tatcaccttc
     4381 aaccagacga tcttggactt gaaaagtcat ctcttgatta agcgaccgca atgtccgact
     4441 tgtggtgacc cggaaattat gcagcggcga gggtttgaac cgctgaagct agaatcgcgc
     4501 cgcaagcggt tcacgcatga tggcggtcac cgtgccacta caccagaaca gactttgcag
     4561 aaataccagc acttgatcgg ccctgtcacc ggagtagtaa cagaactggt gcggatcacc
     4621 gacccagcta atccgttggt acatacgtac cgagcggggc atagctttgg tagtgccacg
     4681 tcactgcgag ggctacggaa taccctgcgc cacaagagtt ctggcaaagg caagaccgat
     4741 agccaatcca gagcgagtgg tttctgtgag gcagtagaac gctattcggg catctttcag
     4801 ggggacgaac ctcgtaaacg agcaactttc gcagaactag cagatttggc gattcatccg
     4861 gcacagtgtt tgcactttag cgatgagcag tataccaatc gggaagcttt gaacgctcag
     4921 ggtacagaag cagcctatcg ttggattcct catcgatttg atgcaagtca agcgatcgac
     4981 tggacgcctg tgtggtcgtt gactgagcag aggcacaaat atctccccac tggcctttgc
     5041 tactaccact atcctatgcc ggaagccaat cgcttctgta aagcggattc caacggaaac
     5101 gcagcaggca ataccctgga agaggcaatc ttgcaagggt tcatggaact ggtagaacgc
     5161 gacagtgtgg cactctggtg gtataaccgc ctcagccgtc cgggggtaga cctgaccagc
     5221 ttcaatgagc cgtattttgt ccagttgcag cagttctacc gagaacaaaa ccgagaactt
     5281 tgggtgctgg atttgactgc tgactttggt attccagcgt ttgtcggcgt atcgtatcgc
     5341 acggtgggaa cttcagagcg gataattgtt ggttttgggg cgcacctcga cccgacgatt
     5401 ggcattcttc gcaccctcac tgaagtgagc caaattgggc tagaactcga caaaattccc
     5461 gacgagcagc tcaaggatga gtcgaaagat tggctgctgg gagtgactag agaaagtcat
     5521 ccttgtttag tcccagatcc atcgcagccg ctgaagaccg ccaatgatta cccgaaacgc
     5581 tggagtgatg acatctatac agatgtgatg acctgcgtga agattgccca aggaataggg
     5641 ttagaaacac tggtgttaga tcaaacgcga ccagacatcg gcttgaacgt ggtcaaagtg
     5701 attataccgg gaacgcgagg cttatggtcg cggtttgggc caggacggct ctatgacgta
     5761 cccgtgaagc tggggtggcg aactgttcct ctggttgaag cagagatgaa ccccatgaat
     5821 ataccgtttt aagttttttc aaaatttacg ggcgacaggc ttcgctacat accagatgtc
     5881 gtcctaataa gcttctttcc aacgaagaag aggattgacc cacctcaacg ggtcaagaaa
     5941 gcgaacagaa aaaagaggaa attactcatg gataagaaga atattctgcc ccaacaaggc
     6001 aaacccgtga tccgcatcac cactggtcaa ctccccagct tcttagctga actctctgaa
     6061 gaagctctcg gtgatgctgg tgtaggagca tccgcaactg gttgtatgtg cgcttacgac
     6121 ggtgcaggag catctgcaac tggttgtatg tgcgcttacg atggtgcagg agcatctgca
     6181 actgcttgtg cttgcgccta cgatggtgca ggagcatctg caactgcttg tgcttgcgcc
     6241 tacgaataag gttgtatagc ctagctcact ttggtggcat gacagttaac cttctgttgc
     6301 tatcgcagtg atttactagg ctgtgagttg ttggcgaggt taaagatgtc gagttgcgag
     6361 caaaacttta ctccatcgtg agttttgtac actttcttca tctacctcgt cttcatctta
     6421 cacttcccag gagactctcg ctacaacgct tcgccgtcta gcagcgtgaa aaataggagg
     6481 ttaatcggca tcaggtcgat gcgagttgcg attaactccc cctagatatt ttttggtaaa
     6541 aagacaatct gtactttgtt agagttaggc actctattga aattagcgac caaaatgata
     6601 gtgatgccat ttaccttact aagatcatga ccctaacttc tatgcttcaa aataatcgtc
     6661 tcaaagaccg ccgtcttcag tttatccgaa ctcatcaaga ggcttttgat gttgagccaa
     6721 cgtttattct ttcgctattt gaagaggcag tacttggaat agaaggtact tgtggtgttg
     6781 aattaatgtg tcatgttgaa ggagaccagt tgtttgcagt tgattttcaa gtttctaatg
     6841 aaagacatac atggcccaga agcttgaccg atgctgtgaa gtttttggat aaagttgaaa
     6901 gtcaagtagg tgttcgactc aatcgtgatt tacttcagca gtttgtagcg gtacacattg
     6961 gctcctctaa aatattaaac aacacgatag gaattgacct tcgaccgaga catgagaatt
     7021 cttgcatcaa ggtatatatg catattgaac atgaagaaga tcctgaagaa ttagtgagga
     7081 cagcgctcaa acttgatggt gatagctatt catctgaaat gctacaggta ttactcaaaa
     7141 gtactattat aattgggttt aatatttttt ttaatggcta cagtgatgtt gagttattgg
     7201 tcgctactgt aggagataaa tacgaaagcc ataaattcaa tcgaggcaaa tatttgaaac
     7261 attacattca aaaaaatttt tcactgaaag ccaattacat gttacgagaa tcgaatatac
     7321 tgctcgtcag tttttctcaa gcaaagaagg taaatccact tttaattttt cattatgagg
     7381 atattaaaga catacggaaa tatttctcgt tcaacagttt gggtgataga agctacagct
     7441 tttttcagag tcaagattgc attacttatg ctggtgtgag tgttagagaa ctcgaattac
     7501 aaaaagatag attagaaaaa ttcagtcttt tttataataa acgtgataaa tgtcaacatt
     7561 tgccattatt ttcaactctc aatgaataat taatattagc tgaacgttgc catcaatacc
     7621 gtggataact accagtacta cctctcagtt ttttagtttc atcgaactcg cgttagtttc
     7681 ttaatcttaa tagacgtttt aaggactaaa gtccatacta cgaactttag tttaattgag
     7741 tccttctact tatgctccat aataatcatc tttaggtgtg tgattttgtc agaatcaaac
     7801 agtaaaatga aatccagaaa ataacactac atattaatct ccacgtcaat catctatagc
     7861 aaacactcta ctatcattga ctcacccagc taatcatatt ttgtaactgt ccatcgccat
     7921 gagcaatcac cccttcgctg tttccctcaa ccctttcgtc caaatcaaaa gcaccgaaga
     7981 ctactacaca ctgcaatcgg tagaatccga ttcaactgct ttgctcaccc ttgctacaac
     8041 gcccggaatc ggtattgctc tcgatcatct caaggcaggc aacaccacca ccgaatcact
     8101 tgtgcaaagt ttaacggaac tttatggcca gggaaccggg gaacagtttg ctctgatgct
     8161 acaacaattg gatgagcggg gttggcttaa ttatgctgta ttgcccctgg cagaggcgat
     8221 tccaatggtg gattctgccg agttaaatct gaccgaaccc tactcgacac aaaccagtct
     8281 cagcctctcc cgctttgcct atcagcattc ctatgatggc aacatggtgc tggagtcccc
     8341 cctctcgaag tttcggatta aacttctcga ctggcgagca agtgcgctac tggctcaact
     8401 cgcccagccc caaactttag gatcgctgac accaccgccc taccttgggc cagaaaccgc
     8461 ctatcagttt ctcaacctgc tgtgggcagc ggggtttcta acggctgacc ctgaaccccc
     8521 ctcgctccag ctttgggact ttcataacct gctattccac agccgcagtc gcaacggtcg
     8581 ccatgattac ccagttatgg atgataacat tgagcagtgg tcaaattttc cagtagtcaa
     8641 gccgcccatg agcgaccgga ttgtgcccct gccgcgtcca aacctgggag cattgatgtg
     8701 taacgatgct acgctcacgc aagcgatcga agtccgaaaa tccgttcgag aatatgacga
     8761 taaccatccg atctccattg agcaagtggg ggaactactg tatcgcaccg cccgtgttaa
     8821 aaaattagtt cgcctagagg atgtagtagg tgaaaatttg caccgaaagc acgaaaattt
     8881 gaaatttggt catgactatg gggagttgag ccgtcgcccc tatcctggtg gcggggtaat
     8941 gtatgaatta gaaatttatc cggtggtagt gcattgtcaa ggcttgaaca aagggatata
     9001 tcactatgac cctctgaatc accagctaga gcagattctc gaatcggagg atgagattgt
     9061 agctctttta ggttatgagc atattacgaa attttttatt agtccgcgtc cacaagtact
     9121 attggtgttt acggcacggt tcggtcggct atttcgtcgc tatcgctcta tggcttatgc
     9181 agcggtgctt aagcatgttg gggtgttaca gcacaacttt tacttggtgg caaccagtat
     9241 gggtttggct ccctgtgcgt tgggggttgg ggatagcgat gcttttggtc gggctatagg
     9301 tctagactat gtagaggagt cggctgtcgg agaatttctg attggctcgc tgcccaaccg
     9361 caatgtagaa gcaagtgggc tggagtcgat tgaagttgat agctcagaag ggttgactca
     9421 gaccattatc tcagttaatg atattgaatt gagtgaaatt gaggctgaac ctgagcctag
     9481 tgaaacagaa gtgttgggaa gtcagcttga gtcagttggt ctagtggcag gaattcagcc
     9541 gactgaggtg tgtgaaagtc ctgaaaatac ctcggcgcaa gttgaggaca gggggactga
     9601 agctgctgat ttggctgctt cagaaacaat ggttgaaaat gcaaattcat ccactcttgc
     9661 tgaaagtgaa agtgatgctg tggctgtttc agtcactgaa gtggaaagct ctgcatccca
     9721 aatatcgctg gcactacacc cccatgacct cgacgatcgc atccctggac tagcccaact
     9781 ccacaaccaa acccttggcg atccccgcat cactattgta attattgacg gtgacccaga
     9841 tcacacttta tcctgttttg ccagagctga agtctctaag gtttttccct actggcacga
     9901 accagcagaa ccaatcagcc cagaacatta tgctagcttc caagcaatca gggacaaagg
     9961 gctgaagggt aaagagaaag aacaggcgat cgacgctgct ttaccaaaaa atgtcaagac
    10021 gcggattgaa atcaacgacc atgcttgcca tatcaccagt atcatagtgg ggcaagaaca
    10081 cagtcctgtt ttcggtatcg ctcccaagtg ccgggtaatc aatatgcccc acgatgcctt
    10141 agcaaaccat gacgatatac aatcaccctt ggagaactat gatgatataa tatcacccct
    10201 caacttagct cgtgccttcg agttagcctt agagttgggt gcaaatatta tccactgcgc
    10261 tttttgtcgt cccacccgaa caagtgttgg ggaagaaatc cttgttaaag caattaagaa
    10321 atgtctagac aataatatct taatcgttgc gcctgtaggg aacaattcaa acaaaaactg
    10381 gtgtctgcct gccgttctac cgggaatttt ggcagtggga gccgcaaaag tcgatggtac
    10441 accagcccac tttagcaact ggggcggcaa caacacacaa gaaggcattc tcgctccggg
    10501 tgtagatgta ttgggggcgc aaccctgcac cgaaaaaccc gtgcgccaga caggaagcag
    10561 catggcagct cctgtaatca caggtatctc tgcactactg atgagtttgc aactgcaaca
    10621 aagaaaaccc gtcgatgccg aagccatccg agccgcactg ctcaacaccg cgattccctg
    10681 cgacccaaag gttgtggaag aaccagaaca atgtctgcgc ggctttgtca atattcctgg
    10741 cgcaatgaag atgctgtttg ggcaaccctc cgtcactttt tccttcgccg gagacggaat
    10801 gacccgcaca gaacgtcctg gatttgccat gcctagcgct atggctaccc cagcaagcat
    10861 caccgccgcc agcccagctg tgcaacccac cgatgccaca acaagcgaaa catccgtcac
    10921 cgccgctgcc gatgccgcag acccaggctc cgttgaagcc agcatcgcct acgatggcaa
    10981 cgtctacgcc ctcggcatcc tgggctacga ctttggggat gaaacccgcc gtgacagctt
    11041 taaggaacta atgcctccta cccaagtcaa cggtatcatg gttccgtcag acccctacga
    11101 tgtccgtcaa atggtccatt acctcgatcg caaccctgat gaaagccgtt ctctaatctg
    11161 gactttgagc ctagatggag acacaatcta cgccctagac ccaacaggac cgttttcagg
    11221 gcagatatac gaaatatttc tgctcatgtt ggcagggcaa ctcgaaccag aaaccagcga
    11281 tgaatttgtg gagcggatca gcgtacccgc ccgacgtact aaccgcacgg tggaactgtt
    11341 ctccggtgag gtcgtacctg tagtgaatgt acatgacctg cgggggttgt acggctggaa
    11401 agtcaatgcc ctagtggacg cggccatggc ggcaatctct cgtcgggtgg acgaagccca
    11461 agcacccctc gtgcgacaag ctttgataac cttcctcaac cgagtttaca acgatctacg
    11521 caatgtgggg caaacttccc gcgatcgcgc cctcaacttt gctgccacca acatattcca
    11581 agcagcttcg gtctttgcta aggcgatcgc tgagcgtcgt caattcgata ccattaaagt
    11641 agaaaaaagc cccttctgcc gcattaacag tgactgttgg gatgtcaaac tagagttcta
    11701 cgacccagaa aatagccgtc gcgggcgcaa agttttccgc tttacccttg acgtgagact
    11761 tctaatgccc gtgacgctgg gtgaagtcaa gagctggtca ctgccgtcta actcaacaga
    11821 c
//
