LOCUS       BGC0000483             14400 bp    DNA     linear   BCT 28-MAY-2012
DEFINITION  Microcystis aeruginosa 9432 WGS project CAIH01000000 data, contig
            AAI_A_2195_263, whole genome shotgun sequence.
ACCESSION   BGC0000483
VERSION     BGC0000483
KEYWORDS    .
SOURCE      Microcystis aeruginosa PCC 9432
  ORGANISM  Microcystis aeruginosa PCC 9432
            Bacteria; Cyanobacteriota; Cyanophyceae; Oscillatoriophycideae;
            Chroococcales; Microcystaceae; Microcystis.
COMMENT     Annotation results relative to BLAST similarities, COG
            assignations, enzymatic function prediction (PRIAM software),
            TMHMM, PsortB and SignalP predictions, and synteny conservation
            (Syntonizer software) are available in the MicroScope annotation
            platform
            http://www.genoscope.cns.fr/agc/microscope.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:43:28
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14400
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14400
                     /db_xref="taxon:1160280"
                     /mol_type="genomic DNA"
                     /note="contig AAI_A_2195_263"
                     /organism="Microcystis aeruginosa PCC 9432"
                     /strain="PCC 9432"
     protocluster    1..14400
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1302:13724]"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1303..13724
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /neighbourhood="10000"
                     /product="cyanobactin"
                     /protocluster_number="1"
     cand_cluster    1..14400
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /kind="single"
                     /product="cyanobactin"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14400
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000483"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14400
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="cyanobactin"
                     /region_number="1"
                     /rules="(cyanobactin_synth or TIGR03678 or
                     cyanobactin_precursor)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1303..5223)
                     /locus_tag="MICCA_2630002"
     CDS             complement(1303..5223)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 77.6; E-value: 1.9e-23)"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:AMIGene:2.0"
                     /locus_tag="MICCA_2630002"
                     /note="Evidence 4 : Homologs of previously reported genes
                     of unknown function"
                     /product="conserved hypothetical protein"
                     /protein_id="CCH92964.1"
                     /sec_met_domain="PF00881 (E-value: 2.8e-15, bitscore: 45.5,
                     seeds: 78, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.2e-21, bitscore:
                     65.9, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 8.2e-178,
                     bitscore: 580.4, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNHHPFAVSLNPFVQVSSTEDQYMLRSVESDPTALLTLATTPGIT
                     MVLDGLKAGSSTPELLVQKLAELEGQAVGEQFGQMLKQLNERGWLSYSVLPLAVAIPMV
                     DSAELNLAQPYWTQTRVALSRFAYQHPYEGTMVLESPLSKFRIKLLDWRASAILTQLAQ
                     PQSLGAVTPPPYLGAETAYQFVNLLWAAGFLTADAEPPSLQLWDFHNLLFHSRSRLGRH
                     DYPTQDIDIEQWSDFPVVKPPMSDRILSLPRPNLVALMSNDATLTAAIEARKSIREYDE
                     AHPITIEQLSELLYRTARVKDILRVEEWFGENWREILGFDADIDYGELGTRPYPGGGAM
                     YELEIYPVVRRCAGLEKGLYHYDPLNHQLEQIIGAEDNIFALSGYKLPNNLPSDLGSPQ
                     VLLVITARFGRLFRKYRSLAYSLVLKHVGVLQQNLYLVATNMGLAPCALGAGDSDIFGQ
                     AAGLNYVEESAVGEFILGSLPNRTMEASGVESVEDVEPSDESAEVAVSVSAVELDRGEA
                     EPEPADVGFRSSTQPTSELDVGFRSSTQPTESVETTEAAVSVSEAESVSQTTTEIMESE
                     ASESEVESDVVVNETESAAISASGVESSATPALVALHPHDLDDRVPGLAHIRNQTLGDP
                     RITIVVLEGNPDHSLSCFQGAEISKVFPYWHDMPEPFSPEDYAKYRAIDDSDRTKQEKE
                     EAFKAAFPEPQIVRLKVDDHTCHVTSIIVGQENTPSAGIAPHCRLINLPVNSTGIPEES
                     IAILNLARAFEQALNLGANIIHCGFCRPTQTCEGDELLAKAVQKCLDNNILIVGPAGNG
                     LGEYWCLPGVLPGVVPVGAMDLGSPPSHYSNWGGNYTEEGIMAPGDDIWGAQLGSEKPK
                     LRTGTSGSAPIVTGIAALLMSLQVQQGKTVDAEAVRTTLLNTAIPCDPNVVEEPERCLR
                     GFVNIPGAMKVLFGQPSVTISFAGDQVTRTEWAGYGTAIAGDTSSIPAGITAIAVSSVG
                     DSTMTMQSATPMAAEVAVTASADPATVEASTAFSGHVYALGTLSYDFGDETCVDTFKER
                     MAPVEMDSILVPPDPYNPRQMVEHLDRNPDESRSLIWTLSLDGDTIYVLEPTGAFSDQI
                     YEMFVLMLVGQLEQESSDEFVERISIPARQTNRTVELFSGQVVPVVKVHDIRGMYGWKV
                     NTLVNAAMVAISRQVDEAQAPLIQQALTSFLNRVYNDLRNVGQTSRDRALNFAATNIFQ
                     AASVFAKAIAERRQLDTIEVEKSPFCRRNSDCWDIKLEFSDPERSSRGRKVFLFTLDVV
                     LQMPVTLGKIKSWSMPSRSEQT"
     gene            complement(5348..6322)
                     /gene="tenF"
                     /locus_tag="MICCA_2630003"
     CDS             complement(5348..6322)
                     /codon_start=1
                     /gene="tenF"
                     /inference="ab initio prediction:AMIGene:2.0"
                     /locus_tag="MICCA_2630003"
                     /product="TenF"
                     /protein_id="CCH92965.1"
                     /transl_table=11
                     /translation="MTITPVFSHHYLREQRLRFIRTHLEAFDVEPRFPLPLFEEAVLEI
                     EGYCGIEPTCHVQGDRIFAGDIQVATYEKTWPKSLMGASKFLDKVGSQVGVQINRNLLD
                     RFSSLYIHSSKIETNTIGIDLRPTIQDSLIKIYMHLHPGGSHEDLVMTALALDGANYSA
                     EVTEVLLKDGALIGFNLFLDGRSNIEIWAISPGGKYQHKGNFGRDLAAYIRKNFSQKVN
                     FLFDVADGIAASFSKQKIDPIFYFHFFDIKDIPKYFAFNSLGDRIYDFYQSQDCVTYAS
                     ISVREQDLESSRLDNYGFLYNKSDVCQLNLDKFHFAQQLGISP"
     gene            complement(6399..8114)
                     /locus_tag="MICCA_2630004"
     CDS             complement(6399..8114)
                     /EC_number="2.1.1.41"
                     /NRPS_PKS="Domain: oMT (50-166). E-value: 2.3e-22. Score:
                     70.5. Matches aSDomain: nrpspksdomains_MICCA_2630004_oMT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089:methyltransferase (Score: 111.5; E-value:
                     7.7e-34)"
                     /gene_kind="biosynthetic-additional"
                     /inference="ab initio prediction:AMIGene:2.0"
                     /locus_tag="MICCA_2630004"
                     /note="Evidence 3 : Function proposed based on presence of
                     conserved amino acid motif, structural feature or limited
                     homology; Product type pe : putative enzyme"
                     /product="putative Sterol 24-C-methyltransferase"
                     /protein_id="CCH92966.1"
                     /transl_table=11
                     /translation="MQNNSISPSVPTQSSPTSEAEKVRAFYNKNSINFSRMVPDPVARS
                     HNLDLAARMGIQPGNYIFDAGCGAGIPAIHIAQSYPGTRIEGITISEVEAAEAQIRIAQ
                     ANLVDRVRVQVGDFHAPPFPDEVFDVVFFNDSIKYSNQVSQVLAEAKRVLRPGGTLYIT
                     GLFVKEPPLSALEQQSLEEAKQQEMYGAHVITIKKTVEFVKQAGFQSIEYDENQAMTMP
                     ALALRLTAANLLHPNSPLFFGEIKAVKPSKTKTQNRIQPSAVISSPVWNRHEENLRYIE
                     EHKQIFDIENSYPLNLFENFIKEQRKGKGGTISCCCNIEKERCYPSRFAYLSEVFVSDP
                     ALKLLDIEDFLSFSHRLEERPELQINRSLLQQFLATGLDPSKVDMVTVGVDVRPELRHS
                     RIKLYLSIRDYPEKIAIAIALCGEQRDWEKLIVNGTLLVGFDFFLDNRAAVEVYPTFSP
                     ADLQRADVQAYLTSRLPAKALPLLYESRTVQIGISNDNDSDILYFNNFKSPNSAVDYLK
                     NETVNQVHAHYRNRVFHDLYLGIPESEFYTNSIQRVKMYYHMNNFVNQKNLRLI"
     aSDomain        complement(7617..7964)
                     /aSDomain="MT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_MICCA_2630004_oMT.1"
                     /domain_subtypes="oMT"
                     /evalue="2.30E-22"
                     /label="MICCA_2630004_oMT.1"
                     /locus_tag="MICCA_2630004"
                     /protein_end="166"
                     /protein_start="50"
                     /score="70.5"
                     /tool="antismash"
                     /translation="AARMGIQPGNYIFDAGCGAGIPAIHIAQSYPGTRIEGITISEVEA
                     AEAQIRIAQANLVDRVRVQVGDFHAPPFPDEVFDVVFFNDSIKYSNQVSQVLAEAKRVL
                     RPGGTLYITGLF"
     gene            complement(8736..11063)
                     /locus_tag="MICCA_2630005"
     CDS             complement(8736..11063)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cyanobactin_D_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NHLP_CD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Goadsporin_CD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) YcaO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03604"
                     /gene_kind="biosynthetic-additional"
                     /inference="ab initio prediction:AMIGene:2.0"
                     /locus_tag="MICCA_2630005"
                     /note="Evidence 4 : Homologs of previously reported genes
                     of unknown function"
                     /product="conserved hypothetical protein"
                     /protein_id="CCH92967.1"
                     /sec_met_domain="Cyanobactin_D_RRE (E-value: 3.7e-44,
                     bitscore: 137.5, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="NHLP_CD_RRE (E-value: 8.8e-23, bitscore:
                     69.0, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="Goadsporin_CD_RRE (E-value: 6.6e-20,
                     bitscore: 59.9, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 8.1e-99, bitscore: 320.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 6e-83, bitscore:
                     267.3, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQSTPLLQIQPHFHVEVIEPKQVYLLGEQANYALTGQLYCQILPL
                     LDGQHNREQIVEKLDGEVPEEYIDYVLERLAEKGYLTEAAPELSSEVAAFWSELGIAPP
                     VAAEALRQPVTLTPVGNISEVTVAALTTALRDIGISVQTPTEAGSPTALNVVLTDDYLQ
                     PELAKINKQALESQQTWLLVKPVGSVLWLGPVFVPGKTGCWDCLAHRLRGNREVEASVL
                     QQKQAQQQRNGQSGSVIGCLPTARATLPSTLQTGLQFAATEIAKCIVKHHVNATAPGTV
                     FFPTLDGKIITLNHSILDLKSHILIKRSHCSTCGDRQILHRQGFEPVKLVSRPKHFTHD
                     GGHRAFTPEQTVQKYQHLVSPITGVVTELVRITDPANPLVHTYKAGHAFGSATSLRGLR
                     NTLKHKSSGKGKTDSQSKASGLCEAVERYSGIFQGDEPRKRATLEELGDLAIHPEQCLC
                     FSDAQYANRDTLNEQATVAHDWIPQRFDASQAIEWTPVWSLTEQTHKYLPTALCYYHYP
                     LPPEHRFARGDSNGNAAGNTLEEAILQGFMELVERDGVALWWYNRLRRPAVDLGSFNEP
                     YFVQLQQFYRENDRDLWVLDLTADLGIPAFAGVSNRKTGSSERLILGFGAHLDPTIAIL
                     RAVTEVNQIGLELDKVPDENLKSDATDWLITAKLADHPYLLPDTTQPLKTAQDYPKRWS
                     DDIYTDVMTCVNIAQQAGLETLVIDQTRPDIGLNVVKVTVPGMRHFWSRFGVGRLYDVP
                     VKLGWRDEPLTEAQMNPTPMPF"
     gene            complement(11394..11645)
                     /gene="mcaB"
                     /locus_tag="MICCA_2630006"
     CDS             complement(11394..11645)
                     /codon_start=1
                     /gene="mcaB"
                     /inference="ab initio prediction:AMIGene:2.0"
                     /locus_tag="MICCA_2630006"
                     /note="Evidence 3 : Function proposed based on presence of
                     conserved amino acid motif, structural feature or limited
                     homology"
                     /product="putative microcyclamide biosynthesis protein"
                     /protein_id="CCH92968.1"
                     /transl_table=11
                     /translation="MRLPLLSPPIKRPHFIQPGLCVDLENGRPEDLVHIRMDLLHAANY
                     NDPAAFANRSFNQVMHSSASQSSASQGWGGFGSMGGFY"
     gene            complement(11751..13724)
                     /locus_tag="MICCA_2630007"
     CDS             complement(11751..13724)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     cyanobactin: cyanobactin_synth"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 164.4; E-value: 8.6e-50)"
                     /gene_kind="biosynthetic"
                     /inference="ab initio prediction:AMIGene:2.0"
                     /locus_tag="MICCA_2630007"
                     /note="Evidence 4 : Homologs of previously reported genes
                     of unknown function"
                     /product="conserved hypothetical protein"
                     /protein_id="CCH92969.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.6e-35, bitscore:
                     111.4, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="cyanobactin_synth (E-value: 1.4e-240,
                     bitscore: 787.3, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDRLHTISQGDSSICVAVLDGPVDQAHPCFQGANLTYLPTLVQE
                     AAKVDGSMSMHGTHVASILFGQPGSPVQGIVPQCKGIIIPIFADDRRRTSQLDLARGIE
                     QAVHAGAHIINLSGGQLTDFGESDGWLKNAVRLCRENNVLLVAAAGNNGCECLHVPAAL
                     PSVLAVGAMDAQGKPLDFSNWGEAYQTQGILAPGEDILGAKAGGGTHRLSGTSFATPIV
                     SGVAGVLLSLQREQGDSPDPQKIRQILLQSALPCDLDLPEESRRCLAGKLNVSGAITLL
                     KGEKMSEEFSSVAASEVTAAGCGCGGTLSATPQEQTAPSLTAVASSPSAGAVPSDTPNL
                     NNLIQSFPTAVTQQPTMPNFTANSVTASQAPSELAEMGQLVYALGTLGYDFGAEARRDS
                     FKQLMPPFDLGGGVMVPANPYDARQMVDYLGENISEARSLIWTLNIELTPVYAIDPAGP
                     FAAESYRALQELLAGQIQPENDGEYVERVSIPGILTGRKVKLFSGQVIPVIEPQSTRGL
                     YGWKVNNLVSAAMDAVQAEDGTADEETIRKTLDGFLNRIYYDLRNLGTTSQDRALNFAV
                     TNAFQAAQTFSQAVAVGMELDSVTVEKSPFCRIDSDCWDVKLKFFDPENSRRAKKIYRF
                     TIDVSDLIPVTLGEVRSWSSPY"
ORIGIN
        1 aggaataaat cttcccagag ggacgggtca tctttagcga agcgaaactg ctgccagttc
       61 gtcaccacta caaacgagtc attggtgaga gtaaggtttt ctcggaagtc gctcggttcg
      121 taaagctcaa aattgaagcg ctctcgccac cgcacaccgg ggggcatgaa taggggttta
      181 ttgatgtcag cttcgccacc caatttaaca gcatctagga ttcttttctg gacttcttta
      241 cccggcgtga cgatgagaaa gcgacggcta aacaatccgg ggcgttcatc gttgagcgcg
      301 ttgaaatagt gccacactag cagggcatta agcacccaag tcttaccgct ccccgtcgcc
      361 attttgagac agtatttagg gaaggtaata ggagcgagtt catcctgtag tgtctttgac
      421 cgtgacgctt ctgctacacg gggatgtcca ggtgcgaact cttgatagag ttggtagggg
      481 ttttggatgc cgagaatttc gtgacagtag atgatggttt cgatcgcctg ttgctggcag
      541 gggtaaaagt tagcgccgtc ccgttcctct tcaaaccaat agcggagcag ttcctcggtg
      601 gttgaggtaa cgccggccca gcctgtattt ctccacccct ctacatcccc ctgtaaaagg
      661 gtgggaatga gcattcctat agtcttggcc tggcctttaa ctggcatggt gtttgctgcg
      721 gggagatgat tctttaatag tatatctgga cttccccgtt gacaaaaggg cataacctgt
      781 gcttgtggta gctataccat acatctaatt taattagata attatattat accaattctc
      841 caaagtctga ctacagaagt ttaatccccc taaatccccc ttaaaaaggg gaacttaaag
      901 ggggatttaa attcgatgtg tagttttcat ttagagaaat ggtattagat gctattctgc
      961 ctaaaagccg ggggaagctg tggcggatcc cttgcccttt gatcgctcgt tttttcttga
     1021 tggtcgattt tttgccttga ctgctatcat caagagacct cttgcataaa tccgaaaaca
     1081 aacgatagaa acaataatgg gagggacttt cagttaatta tttattagta gttgataatc
     1141 ttcaaaaatg ttgctgtaca aggatcgact taagactttt gcaagaggtc taatttcgcc
     1201 gaaaaaatgg gtcatttttg caaaacttcc caaggctgca cctggtgaca cgaaccttct
     1261 gtttacgccg acacttaggt tggtagccca ttttgttgta gatcaggttt gttcagaacg
     1321 cgaaggcatt gaccaactct tgattttacc caaagtgacg ggcatctgaa gcactacatc
     1381 caaggtaaat agaaaaacct tgcgtccccg actactcctt tctgggtcag agaactctag
     1441 cttgatatcc caacagtcac tgtttcgccg acagaagggg cttttttcga cttctatagt
     1501 gtcgagttga cgacgctcgg cgatcgcttt cgcaaagacg gaagctgctt ggaagatgtt
     1561 ggtggcggcg aagttgaggg cgcgatcgcg cgaagtctga cccacattgc gtagatcatt
     1621 gtagactcgg ttgaggaacg aagtcagagc ttgttggatg aggggtgctt gggcttcgtc
     1681 cacctgacga gagatcgcca ccatcgccgc gttgaccagg gtattcactt tccagccgta
     1741 cattcctcgg atatcatgta ccttcacgac gggcacaacc tgaccagaga atagttcaac
     1801 tgtgcggtta gtttgtcggg cggggatgct aattcgttct acaaattcat cactgctttc
     1861 ttgttccagt tgacctacta acatcagcac aaacatctcg tagatctggt ctgaaaacgc
     1921 tcctgttggc tctaggacat agatcgtgtc tccatccagg ctcaacgtcc agatcaacga
     1981 acggctttcg tctgggttgc gatcgaggtg ttcaaccatc tgacggggat tgtaggggtc
     2041 tggcggaacc aagatgctgt ccatttctac cggagccatc cgttctttga aggtatcaac
     2101 acaggtttca tcgccaaagt catagctcaa ggttcccaga gcataaacat ggcccgaaaa
     2161 agcggtacta gcctccaccg tagccgggtc agccgacgca gttaccgcta cttcggccgc
     2221 cattggcgta gccgattgca ttgtcatagt agagtctcca accgaactta ctgcgatcgc
     2281 agtaatccca gcaggaatac tcgacgtatc ccctgcgatc gccgtgccat agccagccca
     2341 ttctgtgcgg gtcacttggt cgcctgcaaa ggaaatggta acagaaggtt gtccaaacag
     2401 caccttcatc gccccaggga tattcacaaa gccgcgtaga cagcgttctg gttcttctac
     2461 aacgtttggg tcacagggaa tagcagtatt cagcagtgtg gttcggacag cttcagcatc
     2521 aacagttttg ccttgttgca cttgcaaact cattaacagc gcagcaattc cggtcacgat
     2581 gggggctgat ccactggttc cggttcgcag tttcggtttt tcactaccga gttgcgctcc
     2641 ccaaatgtcg tcacccggag ccataatccc ctcttcggtg tagttgccac cccagttact
     2701 atagtgggac ggtggactgc ctagatccat tgctccaacc ggaacgactc ctggcaggac
     2761 accaggcaga caccaatatt cacccaaacc attacccgca ggcccaacaa ttagaatgtt
     2821 gttatccagg cacttctgta ccgctttagc caatagctca tccccttcgc aggtttgggt
     2881 agggcgacag aacccacagt gaatgatatt tgcccctaaa ttgagagctt gctcaaaggc
     2941 acgggctagg ttcaaaatag caatagattc ctcgggaatc cccgtactat taaccggcaa
     3001 attaatcagg cgacagtggg gagcaatacc agcagaaggg gtgttttctt gacccacgat
     3061 gatgctggtg acatggcaag tatggtcatc gactttgaga cggactatct gcggttctgg
     3121 aaaagctgct ttgaatgctt cttctttctc ctgtttggtg cgatcgctat catcaatcgc
     3181 tcggtatttg gcatagtctt ctggggaaaa cggttctggc atgtcgtgcc agtagggaaa
     3241 gactttagaa atttcagcgc cctggaaaca ggacagggag tgatcggggt tgccttccag
     3301 aaccacaatc gtgatgcggg ggtcgccaag ggtttggttg cgaatatgag ccagtccagg
     3361 gacacgatca tcgaggtcgt ggggatggag tgctaccaaa gctggggtgg cagaactctc
     3421 aaccccactg gctgaaatag cagcagattc agtttcattc actacaacat ccgattccac
     3481 ttcactctcg gaagcctctg attccatgat ttcagtcgtg gtctgggata cagattcagc
     3541 ctcactcacc gaaaccgctg cctctgttgt ctccaccgat tctgtaggtt gggttgagga
     3601 acgaaaccca acatctagtt cagatgtagg ttgagttgag gaacgaaacc caacatcagc
     3661 aggctcaggt tccgcctcac ccctatccaa ctcaaccgcg ctcaccgaga ctgctacctc
     3721 cgcagactca tccgagggct caacgtcttc aactgactca actccactcg cttccattgt
     3781 tcggtttggc agtgacccta aaataaactc acctaccgcc gactcttcca cataatttaa
     3841 accggctgct tgtccaaaaa tatcactatc tccagccccc aacgcgcagg gagccagacc
     3901 catattcgtc gccaccagat aaagattttg ctgtaacact ccgacgtgtt ttagcactaa
     3961 cgaataagct aaggagcgat acttgcggaa caatcgccca aaccgggctg taataacaag
     4021 aaggacttgt ggcgatccca aatcgcttgg gaggttgttt ggaagtttat agcctgatag
     4081 agcaaagatg ttgtcttctg ctcctataat ctgctccaat tggtgattga gtgggtcata
     4141 gtgatacaac cctttttcca gtcctgcaca ccgccgcact actggatata tctctaactc
     4201 atacatggca ccgccgccag ggtaaggacg ggtgcctaat tcgccatagt caatgtccgc
     4261 gtcaaaaccg agtatttctc gccaattttc accaaaccac tcctccactc gaagaatgtc
     4321 tttgacacgg gcagtacgat agagcagttc actgagttgt tctatggtga ttggatgtgc
     4381 ctcgtcgtat tctcgaatcg atttccgcgc ttcgatcgcg gctgttaacg tagcatcgtt
     4441 actcatcaac gccaccaagt tcggacgtgg cagcgacaaa atccgatcgc tcataggtgg
     4501 cttgaccacc ggaaagtcag accactgttc aatatcaata tcttgcgttg gatagtcatg
     4561 gcgacctaag cggctgcgac tatggaacag caggttatgg aagtcccaaa gctgaagcga
     4621 ggggggttcg gcatcagccg tcagaaaccc tgcggcccac aacaaattga caaactgata
     4681 ggcggtttct gcacccaagt aggggggggg tgtgacggct cctaaagatt gcggttgagc
     4741 cagttgagtg aggatggcac tggctcgcca gtcgaggagc ttgatccgaa acttcgacag
     4801 gggggactcc agtaccattg tgccttcgta gggatgctga taggcaaaac gggagagggc
     4861 aacacgggtt tgtgtccagt agggttgggc tagatttaac tcagccgagt ccaccattgg
     4921 gattgccact gctaggggca agacgctata actcaaccaa ccccgctcat tcagttgttt
     4981 gagcatctgc ccgaactgtt cccccacagc ctgaccttcc agttccgcta acttttgtac
     5041 cagtaattca ggggtactac tgccagcctt gagaccatca agaaccatag tgattccagg
     5101 ggttgttgcc agggtaagta aagcggtggg atcggattct acagagcgca gcatatactg
     5161 atcttcagtg ctgctcactt ggacaaaagg atttagggaa actgcaaagg gatgatggtt
     5221 catggcggtg aataataaga ttaggacaag aatagttgga aaacatcaac ttatgagaag
     5281 ggtttgcaaa gctgtcggct ccgattaatt aatcaagtga tgaaaaatgt ttttgatgtt
     5341 tttatgttta aggactaatt ccaagctgtt gtgcgaagtg gaatttatct aaattcaatt
     5401 gacacacatc acttttattg tataaaaaac cataattatc taatcgacta ctttctaaat
     5461 cttgctctcg aacagatata ctagcatagg taacacagtc ctgactctga taaaaatcat
     5521 aaattctgtc gcccaagcta ttgaacgcaa aatattttgg tatgtctttg atatcaaaaa
     5581 agtgaaaata gaatataggg tctatctttt gcttagaaaa acttgccgca attccgtctg
     5641 caacatcaaa taaaaaattt actttttgtg aaaaattttt tcgtatatag gcagccagat
     5701 ctctgccaaa atttcctttg tgttggtatt ttccccctgg cgaaatcgcc catatttcta
     5761 tgttactccg accatccaag aaaagattaa atccgattaa agccccatct tttagcaaaa
     5821 cttctgtcac ctcagccgaa tagttagcac catcaagtgc gagtgctgtc atcactaaat
     5881 cttcatgaga tcccccagga tggaggtgca tatatatctt gattagagaa tcctgaattg
     5941 tcgggcgtaa gtcaataccg atggtgttag tttcgatttt gctagagtga atgtataggg
     6001 atgaaaaccg atcaagcaag ttccgattga tttgaacacc cacttggctt ccaactttgt
     6061 ctaggaattt tgaagcaccc attagcgatt taggccaagt tttttcatag gtagcaactt
     6121 gaatatcacc tgcaaatatg cgatcgcctt gaacatggca agtgggttca ataccacagt
     6181 atccttcaat ttcaagaaca gcttcttcaa aaagaggtag aggaaagcga ggctctacat
     6241 caaaagcctc cagatgagtt ctaatgaacc gcagtcgctg ctctcgtaga taatgatgtg
     6301 aaaatacagg agttatcgtc ataatggtat ctgcttatga aagatattgt aaatatctaa
     6361 ggtggataat tggtactcat taagagtggg tttggggtct atattaatct caaatttttc
     6421 tgattcacaa aattattcat gtgatagtac attttgactc tctgaattga attggtataa
     6481 aattcagatt caggaatacc tagatataga tcgtgaaaaa ctcgatttcg gtagtgagca
     6541 tgaacttgat taaccgtttc atttttcaga tagtcaacgg cactgttggg agatttaaaa
     6601 ttattaaaat atagaatatc actatcgtta tcattgctaa ttccaatttg aacagttcta
     6661 ctctcataga gtagaggcag tgcttttgca ggtagtctgg aagttaaata ggcttgaaca
     6721 tctgctcgtt ggagatcggc gggggaaaac gtgggataga cttctaccgc agctcgatta
     6781 tccaagaaaa agtcaaaccc tactaataat gttccattaa caattaattt ctcccaatca
     6841 cgctgttccc cacaaagtgc tatggcaatt gctatctttt ctggatagtc cctgatgctc
     6901 aaatacaact taatccgtga atgtctgagt tcgggccgaa cgtcaacacc aacagtcacc
     6961 atatctacct tagaagggtc taaacctgtt gctaaaaact gctgaagtaa actccgatta
     7021 atctggagtt ctggacgttc ttctaggcga tgggagaaag agagaaaatc ttctatatcg
     7081 agcaatttca gggcaggatc ggagacaaaa acttcagaga gataagcaaa acgagaggga
     7141 taacaacgtt ctttttcgat attgcagcaa caggaaatcg ttcctccttt acctttcctt
     7201 tgttctttaa taaaattttc aaataaattt aatggatagg aattttcaat gtcaaaaatt
     7261 tgtttatgtt cttcaatata gcgtaaattt tcttcatggc gattccaaac cggactagag
     7321 ataacagcag aaggttgaat tcgattttga gttttagttt tggaaggttt taccgcttta
     7381 atttctccaa aaaacagagg tgaattaggg tgaagcaaat ttgcagcagt cagacgcaaa
     7441 gcgagggctg gcattgtcat cgcctgattt tcgtcatatt caatggactg gaaacctgcc
     7501 tgcttgacaa attcaaccgt tttcttaatg gtaatcacat gagcgccata catttcttgc
     7561 tgcttggctt cctcaagcga ttgttgctct agcgcagaga gtggcggctc tttgacaaaa
     7621 agacctgtga tatatagagt acctcctgga cgcagcaccc gcttggcttc agctaggact
     7681 tgggacacct gattagaata cttgatgctg tcgttgaaaa acaccacatc aaatacttcg
     7741 tctggaaagg gtggggcatg aaaatcacct acttgcaccc gtacccgatc aactaagtta
     7801 gcttgggcaa tccgaatttg cgcttctgct gcctccactt cagaaatcgt tattccctca
     7861 atccgcgttc ctggatacga ctgggcaata tgaattgccg gaattccagc cccacaaccc
     7921 gcatcaaaga tatagttccc cggttgtatc cccatacgag ccgccagatc aagattatgg
     7981 gatctagcga ccggatctgg aaccattcga ctaaagttaa tggaattttt gttataaaaa
     8041 gcacgaactt tttcggcttc agatgttggg ctggactgag taggaacact aggactgata
     8101 gaattatttt gcataatatt ttgagggtaa tagggggtag ccaaacaagt attaggctat
     8161 acaatcgttt ttcaaaaaag acgaggcaaa gatcaggaag acaagactcg atcgatgtct
     8221 gctatccaag catccttatc ctcgtctttc agctaaagcc tagcgctctt tagtgtttag
     8281 atttaggaca gtaaactgct ttattcccaa aggtgaacta ggctgaagat ttgattggct
     8341 agcgattgcc aattcaggtg atttaggcat caccatcatc gtaggaacaa gggaagaaag
     8401 atgcatctgc cccatcgtag gaacaaacag ggaagaaaga tgcatctgcc ccatcgtagg
     8461 aacaagggaa gaaagatgca tccataccgg caccaccgag cgcttcttca gacagttcag
     8521 ccaagtggct agggagttta ccgttggtgg tacggagtac gggcttgcct tggtggggca
     8581 ggatgttttt cttatccatg agaataatcc tctttttgtt gtttggtttg ttgacccgtt
     8641 ggggtgggtc agtccccttc ttcctggaga agaagcctac tgggacgaca tttgtgatgt
     8701 agcgaaagcg agtcgcccgc aaactttaaa aaaacttaaa atggcatcgg tgtggggttc
     8761 atctgcgcct cagtgagcgg ttcatctcgc caccccagtt tcaccgggac atcatagagc
     8821 cgacccaccc caaaccgtga ccaaaaatgg cgcatccctg gcactgtcac cttaaccaca
     8881 ttcagcccaa tgtccggtcg ggtttgatcg attaccaaag tttctaaacc cgcttgttga
     8941 gcaatgttta cgcaggtcat cacatcggtg taaatatcat cactccaacg cttggggtag
     9001 tcctgggctg ttttgagggg ttgtgtggta tcagggagga gatagggatg atccgccagt
     9061 ttcgcagtta ttaaccaatc tgttgcatca cttttcaggt tctcatctgg gactttatcg
     9121 agttccaggc caatttggtt tacttctgtg accgcgcgga gaatagcaat agtcgggtcg
     9181 aggtgcgccc caaagccgag aatcagccgt tccgagcttc cggttttccg attcgacacc
     9241 cctgcaaaag cgggaatacc caagtctgct gttaaatcca gcacccacaa atcgcgatcg
     9301 ttttcccggt agaactgctg caactggaca aaatacggct cgttaaagct gcccagatcc
     9361 actgccggac gcctcaagcg gttgtaccac cacagcgcca caccgtcgcg ctccaccagt
     9421 tccataaacc cttgcagaat ggcttcttcg agggtgttcc ccgccgcgtt tccgttggag
     9481 tcacctctgg cgaagcggtg ttctgggggt aagggatagt ggtagtaaca cagggctgtc
     9541 ggcagatact tatgggtctg ctctgttaat gaccatacgg gggtccattc aatggcttga
     9601 ctcgcatcaa agcgctgagg aatccaatcg tgagccactg ttgcctgttc gttcagggtg
     9661 tcccggttag catattgggc atcgctaaag cacaaacact gctcaggatg aatcgccaag
     9721 tctcccagtt cttccagggt tgcccgtttg cggggttcgt ctccctgaaa gataccggaa
     9781 tagcgttcca ctgcctcaca caacccacta gccttagatt ggctatcggt tttgcctttg
     9841 ccagaactct tgtgtttcag agtattccgt aaaccccgca gggaggtggc actgccgaag
     9901 gcatgacctg ccttgtaggt atgtaccaag ggattagctg ggtcggtgat tcgcaccagt
     9961 tctgtcacta ccccagttat ggggctgacg agatgttgat atttctgtac ggtctgctct
    10021 ggggtaaacg cacgatgacc gccatcatgg gtaaagtgtt taggacgcga taccagtttt
    10081 acgggttcaa agccctgccg atgcaaaatt tggcgatcgc cacaggttga acagtgcgat
    10141 cgcttaatca agatatggct tttcaagtcc aaaatcgagt ggtttaaggt gataatcttg
    10201 ccatcgaggg tgggaaagaa cacagtaccc ggtgcagtgg cattgacgtg gtgcttgaca
    10261 atgcacttgg caatttcagt tgccgcgaac tgtagccccg tttgcagggt agaaggcagg
    10321 gttgcccgtg cggtaggcag gcaaccgatt acagaaccgg actgaccatt acgttgctgc
    10381 tgggcctgtt tttgctgcaa tactgacgcc tcgacttcgc ggttgccccg tagccgatga
    10441 gctagacagt cccaacaccc cgtcttgccc ggaacaaata ctggacccag ccacagcaca
    10501 ctgcctacag gtttgaccag tagccaagtt tgttgactct ctagtgcctg tttattaatc
    10561 ttggcgagtt cgggctgcaa atagtcatcg gttaagacga cgttgagcgc cgttggagaa
    10621 cctgcttcag ttggggtctg cacggaaata ccaatgtccc gcagagccgt tgtcagggct
    10681 gcaaccgtca cttcactgat gttgcccaca ggggttaggg ttacaggctg ccgtaaagct
    10741 tccgccgcaa ccggaggtgc aatgcctaac tcgctccaaa atgccgccac ttccgaggac
    10801 agttctgggg ctgcctcagt cagataacct ttctcagcga ggcgctccag tacatagtcg
    10861 atgtattctt ctgggacttc tccatccagc ttctcgacaa tctgctcacg attgtgttgc
    10921 ccatccaaga ggggcaaaat ctggcagtaa agctgtccgg tgagggcata gttagcctgt
    10981 tcgccaagga gatagacttg ctttggctcg atcacctcga catgaaagtg gggttgaatt
    11041 tggagcagtg gggtggattg catagaacga acagtcagtg attaagtgga cgataacaag
    11101 accagcagct aggggttaag cccagatgcg accgcgccgg aaggatttag ggtctttgtc
    11161 tgatttcttg gcttgatcct tgacgtattg gcaccagtag gaataatctt gcagcccggt
    11221 ctgggaagtg ggaacgtaag actttttggg ttcaggcgtt gcgatcgtcg ttggttcaga
    11281 cttgcttggt tcagggttag aggggacttc cgaggatgtt gttgtggttt cgttcttgct
    11341 catagtgtcc ttgtatgttt agggttggct gagggcgagt gattagcagc ccattaatag
    11401 aatcctccca tactgccaaa accgccccag ccctggctgg ctgaactttg gctggcacta
    11461 gagtgcatca cttggttgaa actacggttg gcaaaggctg caggatcgtt atagttagcg
    11521 gcatggagca gatccatccg aatatgcacc aaatcttcgg gacgaccatt ttctagatcc
    11581 acgcacagtc ctggctggat gaaatgggga cgtttaatcg ggggagataa tagaggaagt
    11641 cgcatagttt aggattctcc ctatggagag tattaatcaa ctgaacacat ctgcatctcc
    11701 tagtgagagt ggggtctaat attgcactct cactaaaatt gtgcggctcc ttagtaggga
    11761 gaagaccaag aacgaacttc gccaagggtg acaggaatta gatcgctgac atcaatggta
    11821 aagcggtaaa tcttcttggc acggcggctg ttttccgggt cgaagaactt cagcttcaca
    11881 tcccagcagt cgctatcgat ccggcagaag gggcttttct ctaccgtcac gctatcgagt
    11941 tccatgccaa cggctaccgc ttggctgaaa gtttgggcgg cttggaaggc gttggtgacg
    12001 gcaaagttaa gggcgcggtc ttgggaagtg gttcccaagt tgcggaggtc gtagtagatg
    12061 cggttgagga agccatccaa ggttttgcgg atcgtttctt cgtcagcagt gccatcttca
    12121 gcttgtaccg cgtccatcgc tgcgctgacc aggttgttga ctttccaacc gtagagacca
    12181 cgggtgcttt ggggttcaat caccgggatg acttgaccgg agaagagttt aaccttacgt
    12241 ccagttaaaa taccggggat acttacccgt tcaacatatt cgccatcgtt ttccggttgg
    12301 atttgacccg ccaggagttc ttgaagggcg cggtaggact cggcggcgaa ggggccagcc
    12361 ggatcaatcg cataaaccgg agtcagttca atgtttaatg tccaaatcag cgatcgcgcc
    12421 tcggagatgt tttcccctag atagtccacc atctgacggg cgtcgtaggg gttggcggga
    12481 accatgactc ccccacctaa atcaaaggga ggcatgagtt gtttaaagga gtcccgacgg
    12541 gcttctgcgc cgaagtcata accaagggtt cctagagcat aaacaagctg acccatctcg
    12601 gcgagttcgc taggagcttg gctggcagta acggaattag cagtgaagtt gggcatagtt
    12661 ggttgttgag ttacagcagt agggaatgat tgaattaagt tgtttaagtt gggagtgtcg
    12721 ctggggacag ccccagccga tggactggaa gccacagcag ttaagctagg agccgtttgc
    12781 tcttgggggg tagcacttaa tgtgcctcca caaccacaac ctgctgctgt tacttctgat
    12841 gccgccacag acgaaaattc ctcagacatt ttttctcctt ttagtaacgt gattgctcca
    12901 gagacattaa gtttgcccgc cagacaccgc cggctttctt ctggcaagtc aaggtcacag
    12961 ggtaaagcac tctgcaacaa aatttgacga attttttggg ggtcggggga atctccctgt
    13021 tcccgttgaa ggctgagtaa taccccagcg acaccagaaa cgataggagt ggcaaaactg
    13081 gttccactta atcggtgggt tcccccccca gctttggctc ccagaatatc ttcccctgga
    13141 gccaggatgc cttgagtttg gtaggcttcc ccccagttgc taaagtcgag gggtttcccc
    13201 tgggcatcca ttgccccgac ggccagcact gacggtaaag ctgccggaac gtgcaaacac
    13261 tcgcaaccat tattgcctgc tgctgccact aacaagacgt tattctctcg gcacaaacga
    13321 acagcatttt ttaaccagcc atcggactca ccaaagtcag tgagttgacc cccactgagg
    13381 ttgatgatgt gggctccggc atgaacggct tgctcgattc cccgagctaa atctagctgg
    13441 gaagttcggc gacggtcatc ggcaaaaatc ggtatgataa tgcctttgca ttgaggtacg
    13501 attccctgaa cggggctacc gggttgccca aagagaatgc tggcaacgtg agttccgtgc
    13561 atggacatac tgccatcaac tttagcggct tcttgcacca atgtgggcag ataggtaagg
    13621 tttgcgcctt ggaagcaagg gtgagcttga tcaacaggcc catccaaaac agccacacaa
    13681 atactgctat ctccttggga aatagtatgt aatctatcta gcatttaaag gtgttatcat
    13741 ttttacttat gtagcctaga ttcttgacaa aataatggta cctcaaaaat tgtgattaag
    13801 ggggttctga catttaagct aaccataatt tttatcatac catagtttag ttgtagtgga
    13861 ttgtttcagc gaaatcaaaa gttgctctag cccttgtata gtaaaacttt tagcgattat
    13921 taaggattat gaagtgtcac actctagtca gcgatcgctg gcttaatgag agtttatctc
    13981 aataatttgg tatcagagct atcagatttt tagggctaat tgtgcatctg ctgggcaaac
    14041 aaattagcct gtaattcaga gccggcaatc gttttgacga tttagataaa attttcaatg
    14101 tgacacttta gggtgcggaa tgtgggttat tgtttcatgc cgatggaatg gggggggttc
    14161 tcaacatttt tcgcgggtta gacacaatgg ctgaaaatct agagttttcg ttgaggtgcg
    14221 tcgatgcctt gtccagcaag gctttgatcc ttatgaccga ggtaactatt gagaattatt
    14281 attaatagtg tctatgcttt tcaatatccc gcgaaaaatc gttgtacaat ccttatacct
    14341 gaacttatcc catacatata tactataata accgaaaacc cttatccatc aatggatacg
//
