LOCUS       BGC0000484              6789 bp    DNA     linear   BCT 15-FEB-2011
DEFINITION  Lactobacillus plantarum strain KW30 hypothetical protein (gccI),
            hypothetical protein (gccH), UDP-GlcNAc glycosyltransferase family 2
            (gccA), bacteriocin ABC transporter of glycocin F (gccB),
            hypothetical protein (gccC), hypothetical protein (gccD), putative
            LytTR response regulator (gccE), and prebacteriocin glycocin F
            (gccF) genes, complete cds.
ACCESSION   BGC0000484
VERSION     BGC0000484
KEYWORDS    .
SOURCE      Lactiplantibacillus plantarum
  ORGANISM  Lactiplantibacillus plantarum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
            Lactiplantibacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: False
            Run date     :: 2024-10-17 13:43:19
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6789
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /country="New Zealand"
                     /db_xref="taxon:1590"
                     /isolation_source="fermented corn"
                     /mol_type="genomic DNA"
                     /organism="Lactiplantibacillus plantarum"
                     /strain="KW30"
     subregion       1..6789
                     /aStool="mibig"
                     /contig_edge="True"
                     /label="ribosomal"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6789
                     /contig_edge="True"
                     /product="unknown"
                     /region_number="1"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            185..481
                     /gene="gccI"
     CDS             185..481
                     /codon_start=1
                     /gene="gccI"
                     /note="putative glycocin F immunity protein"
                     /product="hypothetical protein"
                     /protein_id="ADV57359.1"
                     /transl_table=11
                     /translation="MADNVLMAYHIVHDPDERAKHVLNTKKLYKWRITEKTKGTPVVGN
                     VALVQTQFAKRTPVMIYATKEVANDLSDLQPVKVFTNNRDQETVNQTFDDLMR"
     gene            complement(903..1259)
                     /gene="gccH"
     CDS             complement(903..1259)
                     /codon_start=1
                     /gene="gccH"
                     /product="hypothetical protein"
                     /protein_id="ADV57360.1"
                     /transl_table=11
                     /translation="MVTNICIIPSAVVGIQESSISGSMIIDSAISAHFTHRLKKSLQGV
                     PNISKIFTLSEFESNWEPNPNTIYIFPDNVYHVLPASFLNKAKYVKFPNSKIFSSDLKE
                     LSHAIKIKLFQYDA"
     gene            1310..2578
                     /gene="gccA"
     CDS             1310..2578
                     /codon_start=1
                     /gene="gccA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /product="UDP-GlcNAc glycosyltransferase family 2"
                     /protein_id="ADV57361.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 6.7e-21,
                     bitscore: 64.0, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKNRQNEIDSYLNLHLRPVHKSFDFGNLTNIDQFRHHIYVSYIVI
                     CKNSQATIERCVNSIAQNMENGDELIVLDTGSTDETVHLVKKNMPQAKISVTNWKNDFS
                     EVRNKALKLASKDWVFYVDSDEWLDVDDGAQLKKILFKVQAKNFKFVINPTFSDHSGQI
                     YQTVGRIFPKKSSFHYYAKIHEEVRKEDQKLGYDVRHFACDDIILYHDGYDKEVLRDKD
                     KIKRNIRLLQEMTCEEPQNARWPFLLARDGFDVLPQDKLKQLVKRTLDLVASDSLQEKY
                     SPFAKKLLGRILLREGKTTQAVLSFKDVLQITGGEDSDAIYYIESFKINEIIAEAKSIE
                     VKMLRYLNKHKGMIDVNSDISGNYYHIAQVILECDIISANYSHLFPLISEIPKNFSGDI
                     KSSVKSAVKLYSKLQGDSKNENN"
     misc_feature    1430..1849
                     /gene="gccA"
                     /inference="protein motif:Pfam:PF00535"
                     /note="Region: glycosyl transferase family 2"
     misc_feature    order(1676..1678,1682..1684)
                     /gene="gccA"
                     /note="metal-binding site"
     misc_feature    2156..2257
                     /gene="gccA"
                     /inference="protein motif:Pfam:PF07719"
                     /note="Region: tetratricopeptide repeat (TPR_2)"
     gene            2565..4673
                     /gene="gccB"
     CDS             2565..4673
                     /codon_start=1
                     /gene="gccB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 167.4; E-value:
                     8.6e-51)"
                     /gene_kind="transport"
                     /product="bacteriocin ABC transporter of glycocin F"
                     /protein_id="ADV57362.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.8e-21, bitscore:
                     63.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIIKQIDQNDCGPAAVATIIGMISKIKIDDWQIRQIITTTNYGT
                     NFISIINGLKSVQVESVVEKCVKNPDVFDEIEFPVLTQIMQNGYLHFVVLTKCSGSKLY
                     WADPGSGKIESIDKAKFMMHWTPLILTIPRSQNFQILKTKVESLKVPILWFSFRKHWAC
                     LLIYWMLSIIIFATTLFLATMYSAYFNRIVPNKFISAIPTITLVYLTVQVASIFLEVIN
                     AILIAYTGNSISKELYEDIFKAFPKKKRLVVDSFEDGEIITRFNAIYQIAGRQLVLLGD
                     LPIDIITMGITLFLLARINPFLTILLFIPMILMALLFYLSSDAIKKRSTNLFKSQETFS
                     QDIIEQIKNFSTIKTFHAGSYINSKITKSMEKYTAANRSFGLYDRLQQIARNGVSQLFG
                     IILFSVGAFLVIKGNIVLGTLLSFYALTGNVLNPFVKIITMQVNLSQGKVATERYLDLL
                     LSPEDNNNKSKLTLSNSIEIRKVAFSYDGVTPIIKNMNGVFSKFPTAIIGNSGSGKSTI
                     AKLMAGFYPAEGLSLGNIPYYAIGAESLGNTITYVEQSPQIFSDTVMNNITLGRKNITV
                     KSINRIASSIGFDECLNHLPNGIYTQLGSSGYSLSGGQKQLLNIIRSMVIPSRFIIFDE
                     ITNGLDINTKLKVENYLLSQNKPKLIFITHDLTLAIRCDDIFTVKNGEMHQIETNKNST
                     ESLREALL"
     misc_feature    2574..2969
                     /gene="gccB"
                     /inference="protein motif:Pfam:PF03412"
                     /note="Region: peptidase C39 family"
     misc_feature    3057..3866
                     /gene="gccB"
                     /inference="protein motif:Pfam:PF00664"
                     /note="Region: ABC transporter transmembrane region"
     misc_feature    4107..4478
                     /gene="gccB"
                     /inference="protein motif:Pfam:PF00005"
                     /note="Region: ABC transporter"
     gene            4670..5089
                     /gene="gccC"
     CDS             4670..5089
                     /codon_start=1
                     /gene="gccC"
                     /product="hypothetical protein"
                     /protein_id="ADV57363.1"
                     /transl_table=11
                     /translation="MKKTIITIIALSVISIIGVYSWQRLTLVQEPLYHSIKATQLQADI
                     KNKKDDVVYFYQKGCEGCEHSTPILNRFIRNKRVTVKAIDINADPSKDFILGTLGISST
                     PTILFIHHGKIVQRINQVFGYEDLTTKYKGYTGDL"
     misc_feature    4676..4738
                     /gene="gccC"
                     /note="Region: transmembrane anchor"
     misc_feature    4775..5053
                     /gene="gccC"
                     /inference="protein motif:Pfam:PF00085"
                     /note="Region: thioredoxin (TRX) family"
     misc_feature    order(4847..4849,4856..4858)
                     /gene="gccC"
                     /note="thioredoxin CXXC motif; active site"
     gene            5090..5623
                     /gene="gccD"
     CDS             5090..5623
                     /codon_start=1
                     /gene="gccD"
                     /product="hypothetical protein"
                     /protein_id="ADV57364.1"
                     /transl_table=11
                     /translation="MNLLKKRKLVLILLILLIILLVIGLFSVIITKRNSYEHENVLRAE
                     IISESKTNTMVYKELNSITIPTFEKEISDKRDFLVYIGRPTCSDCNLFDPILVNELKKE
                     DMTSNVVFLNVAWERQKGWTSWVQFKKKYGFKQTPAIIHYHNGKVLSIIQWGNNKGISK
                     GDLHLWLIKQKEKI"
     misc_feature    5114..5176
                     /gene="gccD"
                     /note="Region: transmembrane anchor"
     misc_feature    5279..5554
                     /gene="gccD"
                     /inference="protein motif:Pfam:PF00085"
                     /note="Region: thioredoxin (TRX) family"
     misc_feature    order(5348..5350,5357..5359)
                     /gene="gccD"
                     /note="thioredoxin CXXC motif; active site"
     gene            5593..6369
                     /gene="gccE"
     CDS             5593..6369
                     /codon_start=1
                     /gene="gccE"
                     /product="putative LytTR response regulator"
                     /protein_id="ADV57365.1"
                     /transl_table=11
                     /translation="MAYQAEGENLVPQTRIFVLTNKPECQNLNNHILQIMLNLSNHIKF
                     CHPIKLSSSSENFQNFICSLSIVILNLDEEIDLIKKISIAVKRLPNSRKYFIYIGSSRH
                     VFENLIFNHYMLYDALMTKTFQKDDGNILAADICNILIHNSIPLNSSNTIQISVKRQKF
                     FLRLDDILFVITDNQPHKVIFHLKYDEYQVRMTLKQVHHQCRNLTACHEGALVNLKNIT
                     KFNKSNRILYFENGDTCEVSRRYTKKISDLIPMKTF"
     misc_feature    6079..6345
                     /gene="gccE"
                     /inference="protein motif:Pfam:PF04397"
                     /note="Region: LytTR DNA-binding domain"
     regulatory      6352..6387
                     /gene="gccF"
                     /note="imperfect direct repeat (N6-AAG-N18-AAG-N6),
                     putative recognition element for DNA-binding domain of
                     LytTR response regulator"
                     /regulatory_class="promoter"
     gene            6352..6782
                     /gene="gccF"
     regulatory      6390..6395
                     /gene="gccF"
                     /regulatory_class="minus_35_signal"
     regulatory      6414..6419
                     /gene="gccF"
                     /regulatory_class="minus_10_signal"
     mRNA            6426..6782
                     /experiment="molecular size of gccF transcript in northern
                     blot"
                     /gene="gccF"
                     /product="prebacteriocin glycocin F"
     sig_peptide     6447..6509
                     /experiment="Edman sequencing of mature glycocin F"
                     /gene="gccF"
     CDS             6447..6641
                     /codon_start=1
                     /gene="gccF"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     glycocin: glycocin"
                     /gene_kind="biosynthetic"
                     /product="prebacteriocin glycocin F"
                     /protein_id="ADV57366.1"
                     /sec_met_domain="glycocin (E-value: 1.7e-51, bitscore:
                     161.2, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKLVKTLTISEISKAQNNGGKPAWCWYTLAMCGAGYDSGTCDYM
                     YSHCFGIKHHSSGSSSYHC"
     misc_feature    order(6522..6524,6591..6593)
                     /experiment="Edman sequencing of CNBr and proteolytic
                     peptides"
                     /gene="gccF"
                     /note="disulfide bond"
     misc_feature    order(6543..6545,6570..6572)
                     /experiment="Edman sequencing of CNBr and proteolytic
                     peptides"
                     /gene="gccF"
                     /note="disulfide bond"
     misc_feature    6561..6563
                     /experiment="Edman sequencing"
                     /experiment="specific hydrolysis of O-glycosidic bond by
                     N-acetyl-beta-D-glucosaminidase"
                     /gene="gccF"
                     /note="beta-O-linked GlcNAc; glycosylation site"
     misc_feature    6636..6638
                     /experiment="chemical modification"
                     /experiment="Edman sequencing of peptides"
                     /experiment="FT-ICR-MS of peptides"
                     /gene="gccF"
                     /note="S-linked HexNAc; glycosylation site"
     regulatory      6747..6782
                     /gene="gccF"
                     /regulatory_class="terminator"
ORIGIN
        1 aaaccaacga ctccagtacc taagtcaacg gttactaaag tagttaagat ggcccaaact
       61 ggtcatcacc aaacttggtt tgatcgattg gtggcttggt tcaaataatt gctagtttga
      121 gggctcagca cgtatactaa tggtgttaag aaatgactac tggccaataa tgggaggaat
      181 caacatggct gataatgtgt taatggccta tcatattgtg catgatcctg atgagcgggc
      241 aaaacatgtt ttgaatacga aaaagttgta taagtggcgg attaccgaaa agacgaaggg
      301 caccccggta gttggtaatg tggcattagt ccaaacccag tttgcgaagc gcaccccagt
      361 tatgatttat gcgacgaagg aagttgccaa tgatttgagt gacttacaac cagttaaagt
      421 ctttaccaac aatcgtgatc aagagacggt taatcaaacg tttgatgacc tgatgagata
      481 atctaacggc ttgtaccaac atgatttttt tcaagttggt acaagccttt ttttataatt
      541 taatacttag taaaatagtg cattttaata tatctgggtg gtcattaaca agtgcattgt
      601 gtaaataatt tgcttaatga ttttaactac aaaaagccaa agagatttgg ctctctttgg
      661 ctttttgtag ttaatgcaga cttgaatccc acttagcatt ttaagagaag cacgtggtaa
      721 tttaaaaccg atgattttgt tcaatcaaat cattaaaata ttttgaatat tgattgtttt
      781 caatccagaa aacaaggaac aaaagaactg ttacagaaat tatggatctt aatatctagg
      841 attgaattga agcatgctgt gcatgtaaat actaaaataa tatcttctga tgtacgatgt
      901 aactaagcat catattgaaa taattttatt ttaattgcat gagataattc cttgagatct
      961 gacgaaaata tcttactgtt aggaaacttc acgtacttag ctttgtttaa aaaagaggcg
     1021 ggtaaaacat gatacacgtt atctgggaaa atatatatag tatttggatt tggctcccaa
     1081 tttgactcga attctgatag agtaaaaatt tttgagatgt tcggaactcc ttgcaacgac
     1141 ttttttagcc ggtgagtaaa atgtgctgag attgctgagt ctattatcat actgcctgat
     1201 atactacttt cttgaatacc aacgacagca gaaggaataa tacaaatatt agtaaccatt
     1261 ttgttacctc ttttcttaaa tacatgatac cacgaaagca ggaatgagaa tgaaaaatag
     1321 acaaaatgaa attgacagtt atttgaacct tcatttaagg cctgtccata aatcatttga
     1381 ttttggaaat ttaacaaaca ttgatcaatt tagacatcat atttatgtat catatattgt
     1441 aatatgtaaa aattctcaag caactattga aagatgtgtg aattcaattg ctcaaaatat
     1501 ggaaaatgga gatgaattga ttgttttaga cactggttca actgatgaaa ctgtgcatct
     1561 ggttaaaaag aatatgccac aagcaaaaat atcagtaaca aattggaaaa atgatttctc
     1621 agaggttaga aataaggcac tcaaattagc atctaaagac tgggtgttct atgttgatag
     1681 tgatgagtgg ttggatgttg atgatggagc tcagctaaag aaaattttat ttaaagttca
     1741 agcaaaaaat tttaagttcg taattaaccc aacattttct gatcattcag gacagatata
     1801 ccaaacagta gggagaatct tccctaaaaa gtcgtccttt cattattatg caaaaattca
     1861 cgaagaagta agaaaagaag atcaaaaact aggttacgat gttaggcact tcgcatgtga
     1921 tgatattatt ctttatcatg atggatatga taaagaagta ttacgagata aagataaaat
     1981 aaagagaaac attcgtttat tacaagagat gacttgtgag gagcctcaaa atgctagatg
     2041 gccgttttta ctagcacgtg atggatttga tgtactgcct caggacaaac tcaaacagtt
     2101 agtaaaaagg accttagact tagttgcttc cgacagtctt caagaaaaat attcgccatt
     2161 tgcaaaaaaa ttgcttggcc gtattttatt aagagaaggt aaaaccactc aagcagtatt
     2221 atcttttaaa gatgttttac agataacagg tggggaagac agtgatgcaa tatactatat
     2281 tgaatcattt aaaattaatg aaattatagc ggaagctaag tctatagaag taaaaatgtt
     2341 gagatattta aataaacaca aaggaatgat tgatgttaat agcgatatat ctggaaacta
     2401 ctatcatata gctcaggtta ttttagaatg tgacataata agtgccaact acagccatct
     2461 tttcccttta atatcagaaa ttcctaaaaa cttttcaggc gatataaaaa gtagtgtcaa
     2521 atctgcagtg aaactgtatt caaaattaca aggtgacagc aaaaatgaga ataattaaac
     2581 aaattgacca aaatgattgt ggaccagcag cagtagcaac aatcattggt atgattagta
     2641 aaattaaaat tgatgactgg cagataaggc aaattatcac aacaacaaat tatgggacta
     2701 attttatcag tataataaac ggattaaagt cagttcaggt tgagtctgtt gtagaaaagt
     2761 gtgtaaagaa tccagatgtg tttgatgaaa ttgaatttcc agtgctgaca caaattatgc
     2821 aaaatggcta tttacacttt gtagttctca cgaagtgttc tggttcaaaa ttatattggg
     2881 cagacccagg tagtggaaaa atagaaagta ttgacaaggc taaatttatg atgcattgga
     2941 cgccgcttat cttaacgata cctagatcac aaaattttca aattctaaaa acaaaagttg
     3001 aaagcttaaa agtccctatt ttgtggttta gttttaggaa acactgggca tgcctgttaa
     3061 tatattggat gctttcaata attatatttg ctacaacact attcttagca actatgtatt
     3121 ctgcctattt taaccgcatt gttcccaata agtttatttc agcgattcct actataactc
     3181 tcgtatattt gactgttcaa gtagcatcaa tttttttgga agttattaat gcaatcctta
     3241 ttgcctatac tggtaattca atcagtaaag aattatatga agacatcttt aaggcttttc
     3301 ccaaaaagaa gcggttagta gtagattcat ttgaagatgg tgaaattatt acaagattta
     3361 atgcgattta tcaaattgca ggtagacaac ttgtgctttt aggtgactta ccaattgaca
     3421 ttattactat ggggataacc ctttttcttc ttgctagaat caatccattt ttgacgattt
     3481 tattgtttat tccaatgata ttaatggcat tattatttta tctaagttca gacgcaatta
     3541 aaaaaagaag tacaaatttg tttaaatcac aggaaacatt tagccaagat attattgaac
     3601 aaataaaaaa tttttcaact ataaaaactt ttcatgctgg tagctacatc aattccaaaa
     3661 taacaaaaag tatggaaaag tatactgcag caaaccgtag ttttggctta tatgatagat
     3721 tacagcagat agcaagaaat ggtgtttcac aactgtttgg aattatttta ttctcggtgg
     3781 gggcttttct agtaataaag ggcaatattg tgcttggaac attgcttagt ttttatgctt
     3841 taacgggaaa tgttctaaat ccgtttgtga aaataataac tatgcaagta aatctgtctc
     3901 aaggaaaagt agcaacagaa cgctacttag atctattact ttctcctgaa gacaataaca
     3961 ataaaagcaa actaacactt tcaaattcta ttgaaataag aaaggtagca ttttcatatg
     4021 atggggttac accaataatt aaaaacatga acggtgtttt ctcaaagttt ccaactgcaa
     4081 taattggcaa tagtgggagt ggcaagtcaa ctattgctaa actaatggca ggtttttatc
     4141 ctgcagaggg tctatcatta ggaaacatac cttattatgc aataggggca gaatctttag
     4201 gtaatacaat tacctatgtt gaacaatcac cacaaatatt ttcggataca gttatgaata
     4261 atataactct tggtaggaaa aatatcacag ttaaatcaat taataggatt gctagtagta
     4321 ttggttttga cgaatgtttg aatcatttac caaacggaat atatacacaa ttaggatcct
     4381 ctggatattc attatctgga ggtcaaaaac aattattaaa tattataagg tctatggtaa
     4441 ttccaagtag gtttatcatt tttgatgaaa taacgaatgg gctggacatc aatacaaaat
     4501 tgaaagtaga aaattattta ttatcacaaa acaaacctaa attaatattt attactcacg
     4561 atttaacatt ggctataaga tgtgatgata ttttcactgt taaaaatggt gagatgcacc
     4621 aaatagaaac taacaaaaac tctactgaat cattgaggga ggcattgtta tgaaaaaaac
     4681 cattatcact attattgcgc ttagtgttat ctcaataata ggagtatatt cttggcaaag
     4741 gctcactttg gtacaagagc cattatatca ctcaataaag gcgacacaat tgcaagcaga
     4801 cataaaaaat aagaaggatg atgtagttta cttctaccaa aagggttgtg aaggatgtga
     4861 acatagtact cccatattga accgctttat tcgaaataaa cgtgtcactg taaaagcaat
     4921 cgatataaat gcagatccaa gcaaagattt tattttggga acattgggaa tatcatcaac
     4981 accaaccata ttgtttatac accatggaaa aattgttcaa aggataaatc aagtctttgg
     5041 atatgaggac ttaacaacta aatataaggg gtacacggga gatctttaga tgaatctatt
     5101 aaaaaaaaga aaactagtat taattttgct aatacttctc attattttat tagtaattgg
     5161 attattttcg gtgattatta cgaaacggaa ttcttatgaa catgagaacg tacttcgggc
     5221 tgaaattatt tcagaatcaa agacaaacac tatggtttac aaggaattaa attcaataac
     5281 aattccaact tttgaaaagg aaatttcgga taaaagagat tttctagtat atattggacg
     5341 gcctacttgt tcagattgta atttatttga tcctatttta gttaatgaat tgaaaaaaga
     5401 agatatgacc agcaatgttg tctttctcaa cgtagcatgg gaacgccaaa agggatggac
     5461 ttcatgggta cagtttaaga aaaaatatgg attcaagcag acacctgcaa taattcacta
     5521 tcacaatggc aaagtattaa gtattataca gtgggggaat aataaaggga taagtaaggg
     5581 agatttacac ttatggctta tcaagcagaa ggagaaaatt tagttccaca gactagaata
     5641 tttgttttaa caaataaacc ggaatgccaa aatttaaata accatatatt acaaataatg
     5701 ctcaatttat cgaatcatat caagttttgt catccaataa aactatcatc aagctctgaa
     5761 aatttccaaa actttatatg ttcactctct atcgtaattt taaatttaga tgaagaaata
     5821 gacttaataa aaaaaattag tattgctgtc aagaggcttc ctaattcacg gaaatacttc
     5881 atttacattg gatcaagtag acatgttttt gaaaacttaa ttttcaatca ttatatgctt
     5941 tatgatgcac taatgaccaa aacttttcaa aaagatgatg gaaatatatt ggctgctgat
     6001 atttgtaaca ttctaataca caattctatt cccttaaata gttcaaacac tatacaaatt
     6061 tctgtcaaaa gacagaagtt ttttctaaga ttggatgata tattatttgt aataactgac
     6121 aatcaaccgc ataaggtaat atttcatttg aaatatgatg aatatcaagt ccgtatgact
     6181 ttaaaacaag ttcatcatca atgtcgtaat cttactgctt gccatgaagg agccttagtt
     6241 aacctaaaaa acatcactaa atttaacaag agcaatagga tcctatattt tgaaaatggt
     6301 gatacttgcg aggtttcaag aagatataca aaaaagatta gtgatttaat cccgatgaag
     6361 acattttagt taccgaataa gatagttgct ctccaatatg tttgtttttg gtttataatt
     6421 ttcttattaa aaggagggta ttaggaatga gtaaattggt taagacactt actataagtg
     6481 aaatttctaa ggctcaaaac aacggtggaa aacctgcatg gtgttggtat actttagcaa
     6541 tgtgtggtgc tggttatgat tcgggaacct gtgattatat gtattcgcat tgttttggta
     6601 taaagcatca tagtagtggt agtagcagtt atcattgtta gttttgtgaa tgttttagac
     6661 ttattaagtt acgttgatag agtatttatt ctttgatttt gtaatttcca caataaagag
     6721 ttatttggaa aatttatgac gcaaataagg aagacaataa cataataatt attgtcttcc
     6781 tttaaattt
//
