LOCUS       BGC0000489              6466 bp    DNA     linear   BCT 18-APR-2005
DEFINITION  E.faecalis plasmid DNA containing gene cluster involved in
            production and immunity to peptide antibiotic AS-48.
ACCESSION   BGC0000489
VERSION     BGC0000489
KEYWORDS    .
SOURCE      Enterococcus faecalis subsp. liquefaciens
  ORGANISM  Enterococcus faecalis subsp. liquefaciens
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:40:06
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6466
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /db_xref="taxon:44244"
                     /mol_type="genomic DNA"
                     /organism="Enterococcus faecalis subsp. liquefaciens"
                     /strain="S-48"
                     /sub_species="liquefaciens"
     subregion       1..6466
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000489"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..6466
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     protocluster    1..5387
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[69:387]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      70..387
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..5387
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     gene            70..387
                     /gene="as-48"
     CDS             70..387
                     /codon_start=1
                     /db_xref="InterPro:IPR009086"
                     /db_xref="InterPro:IPR020038"
                     /db_xref="PDB:1E68"
                     /db_xref="PDB:1O82"
                     /db_xref="PDB:1O83"
                     /db_xref="PDB:1O84"
                     /db_xref="UniProtKB/TrEMBL:Q47765"
                     /gene="as-48"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IId"
                     /gene_kind="biosynthetic"
                     /product="AS-48 protein"
                     /protein_id="CAA72917.1"
                     /sec_met_domain="Bacteriocin_IId (E-value: 1e-33, bitscore:
                     104.5, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVKENKFSKIFILMALSFLGLALFSASLQFLPIAHMAKEFGIPAA
                     VAGTVLNVVEAGGWVTTIVSILTAVGSGGLSLLAAAGRESIKAYLKKEIKKKGKRAVIA
                     W"
     gene            461..2152
                     /gene="as-48B"
     CDS             461..2152
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:O53024"
                     /function="AS-48 maturation and biosynthesis"
                     /gene="as-48B"
                     /product="AS-48B protein"
                     /protein_id="CAA72918.1"
                     /transl_table=11
                     /translation="MNLFGILMKLRANQESSLVKRVFSYFNLKLSLKIYLFINIFFEFS
                     LAFVVSLGLRLTLIKSDINVNLFFIIFFILGIVQGASNCYRTHVFPFEELRKLATISSR
                     KISFIMIVTDLFYIFTFSSSILYGLLSLIYISSNAFIFQKFSLSVFFLFIYICSFYCSN
                     RIFGQYIYNKIVKAIGLTRLIFYSIGAALFTYLGFFIVSFVFSNIVYFIKKYFVNIESV
                     NNKVIWESFSKDIGMFYVNSASKFYESHVYSFTYIDVFLVSAMLLILTILLLAMEPKLY
                     PLKTKMLPKTKIDLCNSYVYFLNKALKKNIFFKCSLLKLANTRWIIANNFFQNIILTYE
                     SFFYIGVMLSIILLNSQNRMLQIQLLFLLNILVIGNQTFEIREEMYPYLSFGLERNQFT
                     LLRSSPNGLNKVFNSKLMTYRLFLIIPLLILIIINIVVSLYIMIPVIFAIFLFITFSMS
                     VYVFPMIQMYMIPLATKLDYTNDTEIGSAKDEKIVLEKFQTVPRYFFNIVPLVLTFIFP
                     IVGERYSLIIFFGELVYFSLATIIFVFFSKKIIRKGIFVVHAIRYH"
     gene            2133..2669
                     /gene="as-48C"
     CDS             2133..2669
                     /codon_start=1
                     /db_xref="InterPro:IPR002798"
                     /db_xref="UniProtKB/TrEMBL:O53025"
                     /function="putative accessory factor in AS-48 secretion"
                     /gene="as-48C"
                     /product="AS-48C protein"
                     /protein_id="CAA72919.1"
                     /transl_table=11
                     /translation="MRLDTINFEKYLKISFYISIFIFFLGVLVGIIIGPHINKLDYFGQ
                     EVSFYSVSINNLKVSFYFLTIGMVTGGIYAFLFMGINGYIIGKLIQYLYINNELNILYK
                     GLLPHFFIELLGLATFSMISTIPIIVILIFFKTPTHVVPIKKIIKLSVLFTVLGIVLII
                     IGGYIESNISYVDIR"
     gene            3041..3535
                     /gene="as-48C1"
     CDS             3041..3535
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:O53026"
                     /function="putative ABC transporter"
                     /gene="as-48C1"
                     /product="AS-48C1 protein"
                     /protein_id="CAA72920.1"
                     /transl_table=11
                     /translation="MHKFTLKKCIGITSLFFLFSNILIAEEFIFVEKNLSFFPKLGIIV
                     LVFFMSAPAFFVVTLILTCLIAFLITLINKFYSFKRVYLSTLFINSIQLFVNLALFSAF
                     LHYNLNLRLLSIMSFMINIFLVAIYRNLLIKFANVNSRAANVLSIFGIVLSIIYLVVGV
                     K"
     gene            3535..4194
                     /gene="as-48D"
     CDS             3535..4194
                     /codon_start=1
                     /db_xref="GOA:O53027"
                     /db_xref="HSSP:1US8"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="UniProtKB/TrEMBL:O53027"
                     /function="putative ABC transporter"
                     /gene="as-48D"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 125.1; E-value:
                     6.1e-38)"
                     /gene_kind="transport"
                     /product="AS-48D protein"
                     /protein_id="CAA72921.1"
                     /transl_table=11
                     /translation="MKKITINNLSFYYESKDTMVFDRLSLEFSSEKIYALVGSNGVGKT
                     TLLNILSGIYQPTGGTIEYDGTLYTEKVTKEKVAFIPYKTKLYPYLDVFDHIKLIAELW
                     GIKTDYLEYKRKVLEYCNRLNLDYYNKRVESYSTGMEYKLYISLMLARDVSLVLLDEPF
                     TMLDKKSRYLAMDLIKEKKIITIFSSHQKDIVEYLSNDIINLDKLKGVNLKNYEKN"
     gene            4181..4351
                     /gene="as-48D1"
     CDS             4181..4351
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:O53028"
                     /function="as-48 immunity gene"
                     /gene="as-48D1"
                     /product="AS-48D1 protein"
                     /protein_id="CAA72922.1"
                     /transl_table=11
                     /translation="MKRIDYIIIIVSLLATIVAIFLIGIDSMLGKIFLAISLGFFSSPF
                     LKWINKLINKN"
     CDS             4556..5065
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:O53029"
                     /note="ORF6"
                     /product="hypothetical protein"
                     /protein_id="CAA72923.1"
                     /transl_table=11
                     /translation="MNDYKKEVKKIKIWNLIALVLISIGTIFSIIGLPSALNPNEKIYT
                     SIGSDGMDIYNQLNSMPVKVFTVLNVLFSIAFVILFFISHRKIKKDQIPVKYPYYLNLG
                     WTVVSIIFSLLTTPSFTNASETQSIGLIIGITTIIFTLVFKLPVIMVLVYLFKVNTGEN
                     DIEKVN"
     CDS             5043..6266
                     /codon_start=1
                     /db_xref="UniProtKB/TrEMBL:O53030"
                     /gene_functions="transport (smcogs) SMCOG1029:RND family
                     efflux transporter MFP subunit (Score: 71; E-value:
                     1.5e-21)"
                     /gene_kind="transport"
                     /note="ORF7"
                     /product="hypothetical protein"
                     /protein_id="CAA72924.1"
                     /transl_table=11
                     /translation="MILKKLTKKQQISLTIIGCLVGVLLISATLIYKLNATEPKEEESY
                     QIIKVKKSDPLVLKGIVQPKTTSYLNFDQSLGKINTISVKNGQEINENDVVATYQNTTV
                     EDQAEEQTQSLEKLNLAVTNAQINLDNATQKQQELENRLSVAKNEQTTIINKKMDDEMK
                     KAEKAESDSKIETIQQALDAQKEAVLQAKQALDAANVDLSSANNTIEQTKKKITTTVTA
                     PFKGIVYINDKGKVDATIPYATIVSPETVIKGSVTEYDYNKVKVGQPVTISQINEEKAT
                     DGIISEINALPEDMAVSPQNTSSTKNSTISTFSFIISPKEPIHYGYNVQISVPMNNLEL
                     AKKNTVKENNNEVFVFIYRDGKVVKQKIEVKEDNDKYVVKTGLKENDSIIENPDTSLKD
                     GQKVTVKQ"
ORIGIN
        1 catcaaaata aactacatgg gtataatagc aatgaaatgc atttcaaaaa tattttgagg
       61 aggagtatca tggttaaaga aaataaattt tctaagattt ttattttaat ggctttgagt
      121 tttttggggt tagccttgtt tagtgcaagt cttcagtttt tgccgattgc acatatggct
      181 aaagagttcg gtataccagc agcagttgca ggaactgtgc ttaatgtagt tgaagctggt
      241 ggatgggtca ctactattgt atcaattctt actgctgtag gtagcggagg tctttcttta
      301 ctcgctgcag caggaagaga gtcaattaaa gcatacctta agaaagaaat taagaaaaaa
      361 ggaaaaagag cagttattgc ttggtaattt aacaatatga taaaaaaaca ggatattttc
      421 tagagatatt ctgtttttta attaaaaaag gggggcgctc atgaatctct ttggaattct
      481 aatgaagtta agagcaaatc aagagagtag tttggttaaa agagtatttt cctactttaa
      541 cttaaaacta tcgttaaaga tctatttgtt tattaatata ttctttgaat tttcattggc
      601 atttgttgtg agtttgggat taagactaac cttaataaaa tcagatataa atgtaaattt
      661 attttttata atatttttta ttttaggaat tgttcaagga gcatcaaatt gttatagaac
      721 acacgtcttt ccttttgaag aattaagaaa attagcgaca atatcttcta gaaagataag
      781 ctttattatg attgttacag acttattcta tatttttacg ttttcatcat caatactata
      841 tggattattg agtttaatat atataagtag taatgctttt atttttcaaa aatttagtct
      901 atctgtattt tttttattta tatacatttg ttcattttat tgtagtaata ggatttttgg
      961 acagtatatc tataataaaa tagttaaggc tattggatta acacggttaa tcttttatag
     1021 cattggagcc gctttattta cttatttagg tttttttata gtttcattcg tatttagtaa
     1081 catagtatat tttataaaaa aatattttgt taacatagaa agtgtaaaca ataaagtaat
     1141 atgggagtcg ttttctaagg atataggaat gttttatgta aatagcgcca gtaaatttta
     1201 tgagagtcat gtatattcat ttacttatat agatgttttt ttagtatcgg ctatgttatt
     1261 aattttgaca atattactat tagctatgga accaaaactc tacccattaa aaacaaaaat
     1321 gctgccaaaa actaaaatag acttatgtaa ttcatatgta tattttttga ataaagcatt
     1381 aaaaaaaaat atttttttta aatgcagctt gttaaagtta gctaatacaa gatggattat
     1441 tgctaataat ttctttcaaa atataatttt gacatatgaa tcattctttt acattggagt
     1501 aatgctttca ataatactac tgaattcaca aaatagaatg ttgcaaatac aactgttatt
     1561 tcttttaaat atacttgtaa ttggaaatca gacatttgaa ataagagaag aaatgtaccc
     1621 ttatctatct tttggattag aaaggaatca atttacgctt ctaagatcgt ctcctaatgg
     1681 tttgaataaa gtttttaatt cgaaactaat gacatacagg ttatttttaa taattccttt
     1741 acttatatta attatcataa atattgtagt ttctttatac attatgattc cagtaatttt
     1801 tgctatcttt ttgtttataa cattttctat gtctgtgtat gttttcccta tgattcaaat
     1861 gtatatgatt cctttagcta ctaaactaga ttacactaat gatacagaaa ttggaagtgc
     1921 taaagatgaa aaaattgttt tagagaaatt tcaaacagtt ccaagatact tttttaatat
     1981 cgttccttta gtgcttacat ttatctttcc aattgtaggg gaaaggtatt cattaataat
     2041 atttttcgga gagttagtat atttctcttt agcaacaata atatttgtgt ttttcagtaa
     2101 aaaaattatt aggaaaggaa tttttgttgt acatgcgatt agataccatt aattttgaga
     2161 aatatttgaa aatttccttc tatatatcaa tatttatttt ttttctggga gtgttagtag
     2221 gaattataat aggtcctcat ataaataaat tggactattt tggtcaggaa gtttcatttt
     2281 atagtgttag tattaataat ttaaaggttt ctttttattt cctcactata ggaatggtaa
     2341 caggggggat ttatgcattt ttatttatgg gtataaatgg ttatataatt ggtaagttga
     2401 ttcaatattt atacattaat aatgaactaa atattttgta taaaggtctt cttccacatt
     2461 tttttataga acttttagga ttggcaacat ttagtatgat aagtaccatt ccaataattg
     2521 ttattttgat tttttttaaa acgcctacac atgtagttcc tattaaaaaa atcataaaat
     2581 taagtgtgct ctttactgta cttggaatag ttttaataat aattggggga tatatagaat
     2641 ctaatataag ctatgtagat atacgataga agttgatata gaaaaactaa tcttaaagga
     2701 aggtatccgt ctatttccaa acgttatact aaagaattta aacaaaccat tgaccacgaa
     2761 ccaaaaatgc tgagttaaag gtatactcat taaaggaaga gtgaacacta ttttaaattt
     2821 atacaaccaa agtaaaattt tcgtgaatta tctttataat aatatgtcgg ttacttaaaa
     2881 tttttgaaat tcacaataaa catataatta acagtgcaat aatttatttg tctacaagtc
     2941 aattgggata ggcaactata ttcgcataaa atatctctca caaaaagtgt ctcatctatt
     3001 gaatctgatc catataagtt cttaagaaag gaagataagt ttgcataagt ttactttgaa
     3061 aaagtgcatc ggtattactt cactattttt tttgttttca aatattttaa ttgctgaaga
     3121 gtttattttt gtagaaaaaa acttgagttt ttttcctaaa ttagggataa ttgtacttgt
     3181 tttttttatg agtgctcccg ctttttttgt tgttacttta attttaactt gtttaattgc
     3241 atttcttatt actttaatta acaaatttta cagtttcaaa agagtatact tatcgacttt
     3301 atttataaat agtattcagt tattcgtaaa tttagcttta tttagtgcat ttttacatta
     3361 caatttaaat ttgcgattgc taagtataat gagttttatg attaatattt ttttagtagc
     3421 tatctatcgc aatttattaa ttaaatttgc gaatgtaaac agtagggctg cgaatgtatt
     3481 atcaattttc gggattgttt tatctataat ttatttagtg gtaggagtaa agtaatgaaa
     3541 aaaataacaa ttaataactt gagtttttat tatgaatcca aagatactat ggtgtttgac
     3601 aggttatctt tagaattttc ttctgaaaaa atttatgcac tagttggttc taatggtgta
     3661 ggaaaaacaa cattgttaaa tattttatca ggtatatatc aacccacagg gggaacaata
     3721 gaatatgacg gcacattgta tacagaaaaa gtaactaaag aaaaagtagc ttttataccc
     3781 tataaaacta agctatatcc ttatcttgat gtttttgatc atataaagct aatagcagaa
     3841 ttatggggaa ttaaaacaga ctatttagag tataaaagaa aagtattaga atattgtaac
     3901 cgtctaaact tggactacta taataagaga gtagagtctt actctacagg tatggagtat
     3961 aaactataca tttctttaat gttggcaaga gatgtttctc ttgtattatt agatgaacct
     4021 tttaccatgt tagataaaaa aagtcgctat ttagctatgg acttaatcaa agagaaaaaa
     4081 ataataacaa tattttcttc acatcagaaa gatattgtag aatatttgtc aaatgatata
     4141 attaatcttg acaaactgaa gggagtaaac ttgaaaaatt atgaaaagaa ttgattacat
     4201 tattattata gtctcactat tagcaacaat agtcgcaata tttttaatag ggatagattc
     4261 tatgttagga aagatttttt tagctatttc tctcggtttt ttttcttccc cattcttaaa
     4321 atggataaat aaattaataa ataaaaatta aaaaaataag aagctgtaca atagaattac
     4381 actacttttt ggtaattttt atagactaat cagcaaaggg agtatgtaaa taattttatt
     4441 tatgtaacaa tatctattag aggaaaccat atgaattatg aaatcttagg aagcttagtt
     4501 aaaatttatt ttttagatag aattctttaa ttatgacaag aaaggatttg aagttatgaa
     4561 tgattataaa aaagaagtaa aaaagattaa aatatggaat ttaatcgctc tagtattgat
     4621 ttcaatcgga acaatttttt cgattattgg cctgcctagt gcattaaatc cgaacgaaaa
     4681 gatttataca agcataggat ctgatgggat ggatatttat aatcaattaa atagtatgcc
     4741 agtaaaagtg tttacagttt taaatgttct tttttcaatt gcttttgtga ttttattttt
     4801 tatttcacat agaaagataa aaaaagatca gattccagta aaatatcctt attatttaaa
     4861 tttgggatgg actgtcgtgt ccattatatt ttctttatta acaacgccta gttttacaaa
     4921 tgcttcagaa acgcaatcta taggactaat tatcggaatt acaactatta ttttcacatt
     4981 agtgttcaag ctaccggtaa ttatggtttt agtttaccta ttcaaagtaa atacagggga
     5041 gaatgatatt gaaaaagtta actaaaaaac aacaaatttc tttaacgatt ataggctgtc
     5101 ttgttggagt attattaatc agcgcgaccc ttatttataa gttgaacgcc actgaaccaa
     5161 aagaagaaga gtcctatcaa attatcaagg taaaaaagag tgatcctttg gttttaaaag
     5221 gaattgttca acctaaaaca actagttatt taaattttga tcaatctctt gggaaaatca
     5281 atactatttc cgtgaaaaat ggtcaagaaa tcaatgaaaa tgatgtagtt gctacttatc
     5341 aaaatacaac tgttgaagat caagcggaag aacaaaccca atctctagaa aagttaaatt
     5401 tggcagttac aaatgctcaa ataaatctag ataatgcaac acaaaaacaa caagaattag
     5461 aaaatcgctt atctgttgct aaaaatgaac aaaccactat tataaataaa aaaatggatg
     5521 atgaaatgaa aaaagccgaa aaagctgaat ctgatagtaa aattgaaacc atacaacaag
     5581 cgttagatgc acaaaaagag gcggttttac aagcaaaaca agcgttagat gcggcgaatg
     5641 ttgatttatc cagtgccaat aatactattg aacaaacaaa aaagaaaatc accactactg
     5701 taacagcacc attcaaagga attgtctata ttaacgataa agggaaagta gacgcaacaa
     5761 taccatatgc gactattgtg agtccagaga cagttataaa aggttctgtc acagaatatg
     5821 attataataa agtaaaagtg ggtcaacctg taacgattag tcaaatcaat gaagaaaaag
     5881 caacagacgg aataattagt gaaattaacg cattgcctga agatatggct gtcagtccac
     5941 aaaatacatc atcaactaaa aatagtacaa tttcaacgtt ctcctttata attagcccta
     6001 aagaacctat tcattatggt tataatgtcc aaatcagtgt tccaatgaat aatttagagc
     6061 tggctaaaaa gaacacagtt aaagaaaata ataatgaggt attcgttttt atttatagag
     6121 atggaaaagt cgttaaacaa aaaatagaag taaaagaaga taatgataaa tacgtagtaa
     6181 agactggact taaagaaaat gattcaatta ttgaaaaccc agatactagt ctgaaagatg
     6241 gccaaaaagt gacggtgaaa caatgattga actcaataag ataaataaat actatgcaaa
     6301 tgaagaggag tcacttcatg tattaaaaga tattcattta tccatcaaaa aaggggaaat
     6361 gatagcgatc atgggccctt caggatctgg caaatcgaca ttaattaatc ttttgggatt
     6421 tatcgatcgt aagtttgagg gagaatactt gtttgaaggc agatct
//
