LOCUS       BGC0000494              7008 bp    DNA     linear   BCT 01-MAY-2007
DEFINITION  Streptococcus uberis PurH-like protein, possible orphan response
            regulator, UblA (ublA), UblB (ublB), UblC (ublC), UblD (ublD), and
            UblE (ublE) genes, complete cds.
ACCESSION   BGC0000494
VERSION     BGC0000494
KEYWORDS    .
SOURCE      Streptococcus uberis
  ORGANISM  Streptococcus uberis
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:40:48
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /db_xref="taxon:1349"
                     /mol_type="genomic DNA"
                     /organism="Streptococcus uberis"
     protocluster    1..7008
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[2437:2668]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      2438..2668
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     cand_cluster    1..7008
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..7008
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000494"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..7008
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             170..1330
                     /codon_start=1
                     /note="ORF3"
                     /product="PurH-like protein"
                     /protein_id="ABG48501.1"
                     /transl_table=11
                     /translation="MLRSAAKNHASVTVVVDPSDYERVLAELTEIGETTYETRQALAAK
                     VFRHTAAYDALIAEYFTAQVGETKPEKLTLTYDLKQEMRYGENPQQAADFYQKALPTDY
                     SIASAKQLNGKELSFNNIRDADAAIRIIRDFKERPTVVALKHMNPCGIGQADTIEKAWD
                     YAYEADSVSIFGGIVVLNREVDKATAEKMHPIFLEIIIAPSYSDEALAILTNKKKNLRI
                     LQLPFEGQTASEIETEYTGVVGGMLVQNQDVIEEKADDWEVVTERQPSDDEKEALEFAW
                     RAIKYVKSNGILIANNHMTLGVGPGQTNRVASVRIAIEQAKDRLEGAALASDAFFPFAD
                     NIEEIAAAGIKAIIQPGGSVRDQESIDAANKHGIAMIFTGVRHFRH"
     repeat_region   1172..1330
                     /note="direct repeat 1"
                     /rpt_type=direct
     CDS             complement(1383..2138)
                     /codon_start=1
                     /note="ORF1; ComE-like; may be involved in regulation of
                     ublA"
                     /product="possible orphan response regulator"
                     /protein_id="ABG48502.1"
                     /transl_table=11
                     /translation="MLSVFILENNFFKQSFLESTIQEVFRKNSWKIRSLKIFGKPEKLL
                     SHISERGSHQIFFLNLKITNDVFKGLEFAQKIRNLDPYAIIIIINIHQNILPLIFQYKI
                     SALDVINEDINDHKFFEHIESLFETCIARLGTSIHQDAISIKNSLSVFQIPFKNILFFE
                     TSDIVHKIKLHTITEKIEFYSSLKQIEKVDKRLFRCHKSFIINPQNVKQIDKTNNLVIF
                     DNGENCFISKLKIKDLTKKFDDLRKKVKE"
     gene            2438..2668
                     /gene="ublA"
     CDS             2438..2668
                     /codon_start=1
                     /gene="ublA"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03651"
                     /gene_kind="biosynthetic"
                     /note="circular bacteriocin uberolysin"
                     /product="UblA"
                     /protein_id="ABG48503.1"
                     /sec_met_domain="TIGR03651 (E-value: 3.9e-28, bitscore:
                     86.6, seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDILLELAGYTGIASGTAKKVVDAIDKGAAAFVIISIISTVISAG
                     ALGAVSASADFIILTVKNYISRNLKAQAVIW"
     gene            2758..4365
                     /gene="ublB"
     CDS             2758..4365
                     /codon_start=1
                     /gene="ublB"
                     /note="putative maturation factor; putative protein
                     involved in uberolysin maturation"
                     /product="UblB"
                     /protein_id="ABG48504.1"
                     /transl_table=11
                     /translation="MEEYKKYFNLICYMIKNNFNQEFYSISQEIKRKLNINFSTIPLFV
                     IFLIYQYSFVLLLLLALYQVRNSTIFNIIFSISMICNMILPGTIARTSTMSISKHPWIE
                     CLQMSNLNKEVTTKIIFWVELVNFWIHNIFMEIISLSLLIIKFKFLGILYCVLWIILVS
                     LIFLRTLSKNSHLSNQTIYPITSYLFNIIFSIILTYKLFEIFIGSLNQTSFSQLLKPNQ
                     IDHYIIIYIKKLIFVFTNQVLTISVIIMALLVLKNVIYIFLKKVYKINEMKIVVILFNK
                     YSDYVKELIKNFWVYRDLRQIFNIITKVDVNPLLLIFPSGIFFIITSCLYYFIRMESHI
                     KILISLVFISWVTLYQYNSFLIQKIPIFNISSELRNCELIKMSKHSIEELISAKKILLC
                     LFSLPILLCVWSIKLLLLPVGINPIILALSVITDMLMCYVSITLVLKWTYIMPNFTWKN
                     IFMIKQDNYDQQMIQHFLLIPGRLITLFFSISFIFVNIVKIDFSIPSLILYFILNIITL
                     SALLFIMKRRVRDEIKRY"
     gene            4346..4891
                     /gene="ublC"
     CDS             4346..4891
                     /codon_start=1
                     /gene="ublC"
                     /note="integral membrane protein; possible accessory factor
                     in uberolysin maturation"
                     /product="UblC"
                     /protein_id="ABG48505.1"
                     /transl_table=11
                     /translation="MKLKGIKNQTNYIMVHLLLFFAGIIIGYLFGHIIEDIGEMPFTLR
                     SYQDYFSHNFSSCLLLIFIGFLTYGLLSWIPLVYNAIVFGVAIKLLLQKYTYLQVIFLF
                     THGIFEIPTIFLSIYLGRVFAIEFKNVIKEIFFEKQIVFCKDNFKKYSLLFLILIFMLL
                     IAAILEENIVKIFEWRIA"
     gene            4876..5532
                     /gene="ublD"
     CDS             4876..5532
                     /codon_start=1
                     /gene="ublD"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 103.6; E-value:
                     2.3e-31)"
                     /gene_kind="transport"
                     /note="ABC-transporter; possible exporter of uberolysin"
                     /product="UblD"
                     /protein_id="ABG48506.1"
                     /transl_table=11
                     /translation="MENCIMLEIVNLNFSYENKEVFHNATFVIKENEVVHLRGDNGVGK
                     TTFYKIISGIYNIEPHLSTEVYINGEKIEMENLKNYVNYLPSIPYLFDYLSGFENIDYL
                     LALFDLEESRDEVLDNIKKLDLFDSLDCEIFKYSLGMKAKLYIGVMIERKCPILIIDEL
                     MSNLDKASQNFILEKLNRKLHESNLSIVMSSHTSEFSSFEEIKQVRIVDKRFVQQ"
     gene            5529..5795
                     /gene="ublE"
     CDS             5529..5795
                     /codon_start=1
                     /gene="ublE"
                     /note="ORF2; possible immunity protein"
                     /product="UblE"
                     /protein_id="ABG48507.1"
                     /transl_table=11
                     /translation="MNKVIGKILLINCIISVFLTVVLPTSEPIGYYLYKSYDIIFSIVN
                     FTLSIFLYFFIVRDKTEDVNSNRLKFLLSITFILSLLTNVVFK"
     repeat_region   5848..6006
                     /note="direct repeat 2"
                     /rpt_type=direct
ORIGIN
        1 tcttttagcc cgtcgcgatt tagatactca tctcaaagca gcacaagaaa acggtattga
       61 acttattgac ctagtggttg ttaatcttta tccgtttaaa gaaaccatct tacgtcctga
      121 tgtaacctat gcccaagctg ttgaaaatat cgatattggt ggtccttcaa tgttacgttc
      181 agcagctaag aaccatgcta gtgtgacagt tgttgtcgat ccaagtgatt atgagcgcgt
      241 gctagcagaa ttaactgaaa ttggtgaaac aacctatgaa actcgtcaag ctttagcagc
      301 taaggttttc cgtcatacgg cagcgtatga tgctttaatt gctgaatatt tcactgccca
      361 agttggtgaa acaaaacctg aaaaactaac acttacttat gatttgaaac aggaaatgcg
      421 ctatggagaa aacccacagc aagctgctga tttctatcaa aaagccttgc caacagatta
      481 ctcaattgcc tctgctaaac aattgaacgg taaagagttg tcatttaata atattcgtga
      541 tgctgatgct gctatccgta ttattcgtga tttcaaagaa cgtccaacag tagtggctct
      601 taaacatatg aatccgtgtg gtattgggca agcagatact attgaaaaag cttgggatta
      661 tgcctacgag gctgactctg tttctatctt tggtggcatt gttgttctga acagagaagt
      721 tgacaaagcg acagctgaga aaatgcaccc tatcttccta gaaatcatta ttgcaccaag
      781 ctattcagac gaagcgctag ctattctcac aaacaagaag aaaaaccttc gcattctcca
      841 attgccattt gaagggcaaa ctgcttcaga aattgagacg gaatacacag gtgttgttgg
      901 tggtatgttg gttcaaaatc aagatgttat tgaggaaaaa gctgacgatt gggaagttgt
      961 taccgaacgt caacctagtg atgacgaaaa agaagctttg gaatttgctt ggcgcgccat
     1021 caagtacgtc aagtcaaatg gtatcttgat tgccaataat catatgactc ttggtgttgg
     1081 acctggtcaa acaaaccgtg tagcttctgt tcgtattgct attgagcaag ctaaggatcg
     1141 tctagaagga gcagccctgg ctagtgatgc ctttttccca tttgcggata acattgaaga
     1201 aattgcagca gctggcatca aagctattat tcaacctggt ggctcagttc gtgaccaaga
     1261 atccattgat gcagcaaata aacatggcat tgcaatgatt ttcacaggcg tgagacattt
     1321 tagacattaa gaaaaagaca gttaaaaaag accaagaaat tggtcttttt taactgtgtt
     1381 tgtcattctt ttactttttt tcttaagtca tcaaactttt ttgttaaatc ttttatttta
     1441 agctttgata taaaacaatt ttctccatta tcaaaaatga ctaaattatt agttttatcg
     1501 atttgtttta cattttgagg atttataata aatgatttat gacatctaaa aagtctctta
     1561 tctacttttt cgatttgttt taagctagaa tagaattcaa ttttttcagt gattgtatga
     1621 agtttgattt tatgtacaat atcagacgtt tcaaaaaaaa gaatattttt aaaggggatt
     1681 tgaaaaactg ataaggaatt ctttattgaa atggcatctt ggtgtatact tgtgcccaat
     1741 cgtgctatgc aggtttcaaa taatgattca atatgctcaa aaaatttatg atcattaata
     1801 tcttcattta ttacatctaa agctgatatt ttatattgaa aaataagagg taagatattt
     1861 tgatgaatat taatgataat aatgatcgca taggggtcaa gatttcgaat tttttgggca
     1921 aactcaagtc ctttaaatac atcatttgtt attttcagat ttaagaagaa gatttgatga
     1981 gagcctcttt cagaaatatg acttaataat ttttcaggtt tgccaaaaat ttttaaagac
     2041 ctaattttcc aagagttttt tcgaaaaacc tcttggattg tgctttctag aaagctttgc
     2101 ttgaaaaaat tattttctaa aataaatacc gataacatgt tactacctca caaataaatt
     2161 ttgtagtccg taacataact aggataataa tataactcaa ctaataataa tagtttaaca
     2221 aaaaaatata aagcctcata gggggaaggg atcaaaaatc tattgtaaat tcaaaattag
     2281 tgtaatatta tattattatg atggaataat tatgttatgt gacaattttg tcgcgtggaa
     2341 acttatatag gttatactat ctttgtccta aacatattag gatcaaaatt gtataaataa
     2401 taattccaca ctgttgtaaa tagaaagggg atttaacatg gacattttat tagaactcgc
     2461 aggatatact gggatagcct caggtactgc aaaaaaagtt gttgatgcca ttgataaagg
     2521 agctgcagcc tttgttatta tttcaattat ctcaacagta attagtgcgg gagcattggg
     2581 agcagtttca gcctcagctg attttattat tttaactgta aaaaattaca ttagtagaaa
     2641 tttaaaagca caagctgtca tttggtaaga agtctttagt ttagttgttg tggaattatt
     2701 atttaacaat ttgaagatgt aactcgacta ataatttgat aaaggagcta aattttgatg
     2761 gaggaatata aaaaatattt taatttgata tgctacatga taaaaaataa ctttaatcaa
     2821 gaattttatt caatatcgca agaaattaag agaaaactaa atataaattt ttctacaatc
     2881 ccattattcg ttattttttt gatatatcaa tattcatttg tacttctatt actgttagct
     2941 ctatatcaag ttagaaattc cacgattttt aatataatat ttagtatttc aatgatatgc
     3001 aatatgatat taccaggaac tattgcaagg acaagtacta tgagtatttc gaaacaccct
     3061 tggatagaat gtctacaaat gtcaaatttg aataaagaag taacgacaaa aatcatattt
     3121 tgggtggagc tagtgaactt ttggatacat aatattttta tggaaatcat ttcattgagt
     3181 ttattaatca taaagttcaa atttttagga atactttatt gtgttttatg gataatttta
     3241 gtttcactta tctttttaag aacattaagt aaaaatagtc atttatcaaa tcaaacaatc
     3301 tatccaatta cttcctatct ctttaatatc attttcagta tcatattaac ttataagtta
     3361 tttgaaatct ttatcggaag tctaaatcaa acctctttca gtcaattgct taaaccaaac
     3421 cagattgacc attatattat tatctatata aaaaaattaa tattcgtttt tactaatcag
     3481 gtattaacta tttcagttat tataatggca ttattagtct taaaaaacgt aatctatatt
     3541 tttctaaaaa aagtctacaa aatcaatgaa atgaaaatcg ttgtaatatt atttaataag
     3601 tattctgact atgttaagga attaataaaa aatttttggg tatatagaga tttaaggcag
     3661 atttttaata ttattacaaa agtagacgtc aatcctttgc tacttatttt tccttcaggt
     3721 atttttttta taataacatc atgcttatat tactttataa gaatggaatc acatattaaa
     3781 atccttattt ctttagtgtt tatatcatgg gttaccttat atcaatacaa ctcatttttg
     3841 attcaaaaaa tcccaatttt taatatatcc agcgagttac gtaactgtga attgataaaa
     3901 atgagtaagc attctataga agagttaatt agtgctaaga aaatcctact ttgtttgttt
     3961 agtttaccta tattgttatg tgtatggtct attaaactgt tattacttcc agttggtatt
     4021 aacccaataa tattagcttt aagtgttatc acagatatgt taatgtgcta cgtttcaatt
     4081 actctagttt taaaatggac atatattatg cctaacttca catggaaaaa tatatttatg
     4141 attaaacaag ataattatga tcaacaaatg attcaacatt ttttacttat tccagggaga
     4201 ttaattaccc tatttttttc aattagtttt attttcgtaa atatcgttaa aattgatttt
     4261 agcataccgt cactaattct ttattttata ctaaatatca ttacattatc agctctgcta
     4321 tttattatga aaaggagagt cagagatgaa attaaaaggt attaaaaatc aaacaaatta
     4381 tataatggtt catctactat tattttttgc aggtattatt attggttatt tgtttggaca
     4441 tattattgaa gatataggtg aaatgccttt tacactaaga agttatcagg actattttag
     4501 tcataatttt tcgtcatgtt tattattgat ttttatagga ttcctaacat atggcctttt
     4561 gtcgtggata cctttagttt ataatgcaat agtatttggt gtggctatta aattattgct
     4621 acaaaaatat acttatctac aagttatttt tttattcaca catggtatat tcgaaatccc
     4681 aacaatattt ttatctattt atttaggtag agtatttgca atagagttta aaaatgttat
     4741 taaagaaatt ttttttgaaa agcagattgt tttttgcaag gataatttta aaaaatatag
     4801 cttactattt ttaatcctaa tttttatgtt attaatagca gctattcttg aagaaaatat
     4861 agtgaaaata tttgaatgga gaattgcata atgttagaaa ttgttaactt aaattttagt
     4921 tatgaaaaca aagaagtttt tcataatgct acgtttgtca taaaggagaa tgaagtcgta
     4981 catcttagag gagataatgg tgttgggaaa acaacatttt ataaaataat ttcagggatt
     5041 tataatattg aacctcattt atctactgaa gtatatataa atggggaaaa aatagaaatg
     5101 gaaaatctta aaaattatgt caactatctt ccaagtattc catatttatt tgactattta
     5161 agtggttttg agaatatcga ctatttatta gcgttatttg atttggaaga aagtcgtgat
     5221 gaggtactag ataacataaa aaaattagat ctatttgatt cactcgattg tgaaatcttt
     5281 aagtattccc ttggaatgaa agcaaagcta tatattggtg ttatgataga aagaaagtgc
     5341 ccaattttaa tcatagatga actgatgagt aatttggata aagcttctca aaattttatt
     5401 ctagaaaaat taaatcgaaa attacacgaa agtaatctat caatagttat gtcatcacat
     5461 acaagtgaat ttagtagttt tgaagagatt aaacaagtta gaatagttga taagaggttt
     5521 gttcaacaat gaataaagta attggaaaaa tattattaat aaattgtatt atttcagtgt
     5581 ttttaaccgt tgtattacca acatctgagc caataggtta ttatctttac aagagttatg
     5641 atattatttt ttctattgtt aatttcacat tatcaatatt tctttatttt tttatagttc
     5701 gagataagac ggaagatgtt aattcaaata ggttaaagtt ccttttatct attactttta
     5761 tactttcttt attaacaaat gtggtattta aatagatagt cctcttctat taaacagagt
     5821 gaggttcttt gcctttttca aggaatattt ttcccatttg cggataacat tgaagaaatt
     5881 gcagcagctg gcatcaaagc tattattcaa cctggtggct cagttcgtga ccaagaatcc
     5941 attgatgcag caaataaaca tggtattgca atgattttca caggcgtgag acattttaga
     6001 cattaatcat acaaatcctc aggttttatc tgaggatttt tgtgatcttc ctattaagct
     6061 ttctgtcaaa atctttaagg tctaattaag caatgaatct tatactagta ctatcctagc
     6121 aataggaacc ctaataattc ttttttcata aacctcctaa gacttaaaaa actagctttt
     6181 aatggctagt ttttttattt ttaaggagat gtgttagata gatggataga ttatcttact
     6241 tattttgtaa aattttaact tattctattg acttgtaatt aataatataa tatactctta
     6301 agtgtttata accagttgga ggagaattga tgacaagcca aagaccttta tacatgcagt
     6361 tggttgacat gcttgagtta aaaataagag aatccatgtc accaaatgac aaattattat
     6421 ctgagcgtga attgagtgag cattatggtg taagtcgtat tacagtacgt cttgctttga
     6481 aagaactaga aattagaggg ttaatctaca aaaaacaagg caaaggaaca tacgtttcag
     6541 gtatcaatga acctgcaaca gatttatcag ctgcttatag ttttaccgaa gaaatgagaa
     6601 aacaaggacg tgaacctaaa acaacaatct tatcgtttga aaagttaacg gttactccct
     6661 atttatcaac tttattagac gtgaataaag ggactgaaat ttttgaactt gaacgtctac
     6721 gtttagcaga tgataaacca ttaatgttag aaagaacttt tctacccttt caaaaattct
     6781 caactctttc tgaagagatg ttaagacaaa aacctctata cgatattttt tcagaggatt
     6841 atcatgagat ggttcgtttt gctgaagaag aattctatgc gagcatagct ttggattacg
     6901 aggcatcttt attggaaatt aaaaaaggag acccggttct tcatgttata cgcaaaacct
     6961 ataatgaaaa aaatgtttta attgaataca cctttagtat gctagggc
//
