LOCUS       BGC0000497             11249 bp    DNA     linear   BCT 31-OCT-2005
DEFINITION  Streptococcus ratti two-component lantibiotic gene locus, complete
            sequence.
ACCESSION   BGC0000497
VERSION     BGC0000497
KEYWORDS    .
SOURCE      Streptococcus ratti
  ORGANISM  Streptococcus ratti
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:44:35
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /db_xref="taxon:1341"
                     /mol_type="genomic DNA"
                     /organism="Streptococcus ratti"
                     /strain="BHT"
     protocluster    1..11249
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1422:8380]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1423..8380
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..11249
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..11249
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000497"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..11249
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            229..1092
                     /gene="bhtR"
     CDS             229..1092
                     /codon_start=1
                     /gene="bhtR"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR01716"
                     /gene_kind="biosynthetic-additional"
                     /product="BhtR"
                     /protein_id="AAZ76596.1"
                     /sec_met_domain="TIGR01716 (E-value: 1.3e-50, bitscore:
                     161.1, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDQEQLMQLGELYRDLRIARGLKLKDVARENLSLSQLSRFENGQT
                     MLTADKLLLAIEAIHMTFPEFSYAVNHYQETIYFKRSQQLAQLYNQHDIDGLKTMLEDI
                     EGDEVFDVYEKLNKLVVKVALFNLDKSYQVPDDDKKLLVNYLYAIEEWTTYELYIFGNT
                     MTALSNEDLIFLGKAFIERDKLYSSIPTHKLLSQQTLQNLSLVLLERREFYYTRYFISH
                     LRQLITFQDIFTKINIDFYEKLLDHMETGATTMADLEQFIDRVKELADPIFPMFLRENL
                     RQILEV"
     misc_feature    229..10998
                     /note="two-component lantibiotic gene locus"
     gene            1423..4224
                     /gene="bhtM1"
     CDS             1423..4224
                     /codon_start=1
                     /gene="bhtM1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     623.9; E-value: 8.4e-189)"
                     /gene_kind="biosynthetic"
                     /product="BhtM1"
                     /protein_id="AAZ76597.1"
                     /sec_met_domain="DUF4135 (E-value: 1.3e-69, bitscore:
                     224.2, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 2.2e-70, bitscore:
                     226.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCLLEDINASINELEVYEIWNRFHLLSSNTFSNLYLSFLEPYLFE
                     IRNQLQKYDDSFQSDTIERTITENMLENIYSLSEGIWIAEMRNINKNQAEYLAYLNQDY
                     KNLAEVVKKYPYWYFSIILFIRDTTKYLLEFLSHLIQDKALVANQFLEADGMITDIKLS
                     MGDRHRGKFVIKVESEFGNYFYKPRRSKLDTLFKEVIDKIGDQDGILKMKAPKIIDQGS
                     YSWAEEIRYIPLQSKNKNERYYIRLGQLLAIAYILNGSDLHYENIISCGEFPVAIDVET
                     LLTSRLRFKKQMTPKMLQEDNINYLEDSVSNLIILPTIVKSNGDFYDLSPLKIYSGELP
                     NTFAETWKKSNHLVQKNELKDVIDFIQRGFTNIYNEVLKDKERYKEYFNRLFNNIPIRY
                     LNRETHDYSQILRLLKNPVCLFHFKYAFAVSMKLLSEEIQEEEVQEYKEMLKFNIPYFE
                     LNTGSTDLLLSNGEKIKNYFIETPLESLEYKLNLLCEEDLERQLRLITQMLTVVSTEFD
                     VKEINEVTIHEGENPQKQRAQTIEQMIATVFENKLINPLTKQYFWITPELEGDNNRSFY
                     QIHENPDSYYSGSVGILRCLLLLEKSGYKDLPINKLISDIEIDIDKIIKYGTSDISIGA
                     YNGVVEYLRYYAYIYKHNNFSDVLSLKRIHYLLDLIENSFKKDEKLDILDGTAGVILTL
                     IEIFQLHLSDCLNNRVIKLVEKCRLHLIDSIIYDKGEVYFPIAQEKKRYFTGFAHGSSG
                     ITLAIYRANKLLDIDDYTLIQSLLKTERRLFDKTNQMWFKDNNRNSYSWGWCHGIPGIL
                     LSRLELIKHGYQDDYIIEEAYELYEIAMRKSLGVNLTFCHGDLGNLVICKYAEAILNIE
                     YSKVEIYIQEILPYLLNSMEYNIRGTETVGVMHGLVGIATFLDSTICDNYLRLLDILKI
                     TSD"
     gene            4235..5059
                     /gene="bhtF"
     CDS             4235..5059
                     /codon_start=1
                     /gene="bhtF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 155.5; E-value:
                     3.1e-47)"
                     /gene_kind="transport"
                     /product="BhtF"
                     /protein_id="AAZ76598.1"
                     /transl_table=11
                     /translation="MKILEIQNVNFKYGKDYVLKNINLTLEQGDILGLVGENGAGKSTL
                     MKLISGIIPNYKGDIKIQAHNVGTLIEHPSLYADMTVLGNLKFYCRLFGKSYSVIDDYK
                     YVLQVDSYLHKKVSKLSLGMKQRVGLFIALIASEEFILLDEPTNGLDPTGIKNLLDLIK
                     KLSSEKGITFIISSHILQNLEQICNKAVLLRDHTISSLDAKEHMKYKIYHPDLSQSELI
                     CLLEDNGFDYEQNGRDIIVRDIDAIEEMLQREKNITLEKEKISLSEVYFDEQ"
     gene            5049..5765
                     /gene="bhtE"
     CDS             5049..5765
                     /codon_start=1
                     /gene="bhtE"
                     /product="BhtE"
                     /protein_id="AAZ76599.1"
                     /transl_table=11
                     /translation="MNNKWATVLFVRLTIVKFSVFIFLMLSLLLGGGIIYSIENLKGPF
                     QVYNIYSVFSTVSNFLLMYAAINAFGREFRYKTINQLRISGRSSIEIILRKLLAVELLA
                     ILTSLVSFAEVAFYKIYFNHPQIDLFEIFNHLVPAYLVYALFLFSLGSIITLVLKSSLY
                     SFITLFLTLRLGVTIMNVMNNFESTADLTKYIPLSFVENAFSFAKYTPEQYVVTIVWSV
                     ALMALLPVIYRKWGYA"
     gene            5750..8380
                     /gene="bhtM2"
     CDS             5750..8380
                     /codon_start=1
                     /gene="bhtM2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     268.3; E-value: 2.2e-81)"
                     /gene_kind="biosynthetic"
                     /product="BhtM2"
                     /protein_id="AAZ76600.1"
                     /sec_met_domain="DUF4135 (E-value: 2.3e-48, bitscore:
                     154.3, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 5.3e-17, bitscore:
                     50.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGLCIVENIRNSAYPSIEGKFENYFSHYISKIYEQLLSIDSKGLF
                     SSQKDQDNCYYTLIDGILENISSVAINSLIDYYQEERAINPNLSYKDYNENLQKDRYFQ
                     EFHQIYPTVKDNCEVVICNYIQRIKEVFSLLQNYQVEIRNFASLKESESLSLSKLNTFE
                     GDFHINTFVAILTLNEKRILIKKRPNLGDRLLRIFQKYFDKENIKLPIANTIFLDDDLC
                     LQEYIKSDSAVLNSIELQNFYYKFGIITAIFTILGSKDLHSENIISTKYGPYFIDLEAA
                     ITDTLNREKLDSLLTETYLFNSREKRLVYGSLDLSAFSGGDITTWRHGVIHKGQDDINL
                     GVYSYTEKHSNIPQDINGIKVNPEEYSADVLKGFDTGIRVFLKYKERFIQELELIESYD
                     YRVVLRNTAFYGKYLDDLNLPMYTKSKEKSENYINLLRKHSKRDKKIVDKEMACLKQQA
                     IPYFLISEVTDNKIIKDNLIKRIGGLSLNDLNKEKYYLQLILGLDIPIERESHYLDNRN
                     RVDNELQAFESFCSNDMGFKFGVIDHTVQNTLLHYCNDIYTFGGSLLFLASYCKNSKLR
                     EKIIATVRSGDTSEGLSGLIGRQSSYLLYHYFNLESEKQNYTSSKDIVHEKDIVDFSTY
                     GSAILVLNYLYKETSNKAYLDDIKLLGEIYLKTYTKRNLTGLFHGYAGDILVLSVLRQY
                     IEHQTIDKDILDLIQLENSNFNKVSNNWNDTRHNISNTEDLCALSYGAVGILFSRYLLY
                     RSSNLSKKLKDIVITDLFKALNCILVRKRSDYMDDSLVNGYAGALVVLKIILQSGLLNN
                     HYSKVIKVKTYIEEGEKLLENSQWRYEQFSNLLNPSFFNGRLGTVFTLWFISQNDFYIE
                     KFLGV"
     gene            8417..10513
                     /gene="bhtT"
     CDS             8417..10513
                     /codon_start=1
                     /gene="bhtT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 230.6; E-value:
                     6.2e-70)"
                     /gene_kind="transport"
                     /product="BhtT"
                     /protein_id="AAZ76601.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.4e-30, bitscore:
                     95.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQTECGLCVAVMLMEYYDVRLTLNDIPKEFSAGRDGTRIRGLKK
                     IFDHFGFSMNMFKVTDRLSEIKNSSLPGIAYRIGGHFVVVESISSNEVIILDPAFGRTK
                     ISKDELDNDYKNLILKITPNENFKTVNTRGQEFSLIKEAVFFNKKLVVQVLIAAVIVYS
                     LTLIAPLLLKGLVDNFLVSKQIDTTIWALSIGTIASSLVYFVVNRVKQITSIRLSISVD
                     KFLSKKVVDKLFKNKFEFYLNRTSSDIQYRLVLLKNLKMIISDIIIQTFLDIGSMVVIL
                     IYIMNLQPLYAVFLAFITLLVLSMSRFMREKMLLYKNKELATDSKLQVFQYDIFRAIFD
                     VKVLGISSKKHRSWHRYYEDYIKSHEKNQRFITLYQNILAYITLYVPIFIPILGIWISQ
                     LDHSNQVGTIISLQSITGTYISGLISIAQLSDNITTAKSYLIRIDDILLQEDEEVRNRI
                     IDFKGNIRVNNLSFAYPGAEDKTLDDISFSINEGESVAIVGESGSGKSTLFYILLGAYD
                     NYEGDIYFEGLDLKILNKDALREQIGVVPQDALLFNGSIKENLVEDFEVADNDIYDALK
                     KVTLLDFVDSLPMGINTVVSENGFNFSGGQRQRLALARAILNQKSMLFLDEATSALDNI
                     TEHQIVEYLSEDSKTKIVIAHRLSTIKNSDKIIVLKDGQVSEVGDHRSLMARKGEYYRM
                     YSGK"
     gene            10612..10800
                     /gene="bhtA beta"
     CDS             10612..10800
                     /codon_start=1
                     /gene="bhtA_beta"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="BhtA2"
                     /protein_id="AAZ76602.1"
                     /transl_table=11
                     /translation="MKSNLLKINNVTEVEKDMVTLIKDEDMELAGGSTPACAIGVVGIT
                     VAVTGISTACTSRCINK"
     CDS_motif       10612..10707
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence:
                     MKSNLLKINNVTEVEKDMVTLIKDEDMELAGG"
                     /locus_tag="bhtA_beta_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       10708..10800
                     /prepeptide="core"
                     /locus_tag="bhtA_beta_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="2.60"
                     /molecular_weight="2750.2"
                     /monoisotopic_mass="2748.5"
                     /core_sequence="STPACAIGVVGITVAVTGISTACTSRCINK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKSNLLKINNVTEVEKDMVTLIKDEDMELAGG"
                     /leader_location="[10611:10707](+)"
                     /alternative_weights="2768.3"
                     /alternative_weights="2786.3"
                     /alternative_weights="2804.3"
                     /alternative_weights="2822.3"
                     /alternative_weights="2840.3"
     gene            10813..10998
                     /gene="bhtA alpha"
     CDS             10813..10998
                     /codon_start=1
                     /gene="bhtA_alpha"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_ha"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="BhtA1"
                     /protein_id="AAZ76603.1"
                     /sec_met_domain="mature_ha (E-value: 4e-17, bitscore: 50.8,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKEIQKAGLQEELSILMDDANNLEQLTAGIGTTVVNSTFSIVLGN
                     KGYICTVTVECMRNCQ"
     CDS_motif       10813..10899
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKEIQKAGLQEELSILMDDANNLEQLTAG"
                     /locus_tag="bhtA_alpha_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       10900..10998
                     /prepeptide="core"
                     /locus_tag="bhtA_alpha_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-3.00"
                     /molecular_weight="3325.9"
                     /monoisotopic_mass="3323.7"
                     /core_sequence="IGTTVVNSTFSIVLGNKGYICTVTVECMRNCQ"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKEIQKAGLQEELSILMDDANNLEQLTAG"
                     /leader_location="[10812:10899](+)"
                     /alternative_weights="3343.9"
                     /alternative_weights="3361.9"
                     /alternative_weights="3379.9"
                     /alternative_weights="3397.9"
ORIGIN
        1 ggtaccgaat aaattggtca atattgtggt taaataaaaa tatgaggact ggacaagttt
       61 ttgctcagtc ttttttagaa aaagcatatt tccgaataat aaagtgagat ttctatcgaa
      121 aattttaacc attcaggaca aatgacgact attcacgaat agacaaataa aaacagggat
      181 atttgctata atctgagtac cctatctata tgataaagga ggctgtcaat ggaccaagaa
      241 caattaatgc aattgggtga attataccgt gaccttcgta tagcgcgtgg gctcaagcta
      301 aaggatgtcg ctagagaaaa tctttccttg tcccagctat ctagatttga aaatgggcaa
      361 accatgctga cagcagataa gctgctatta gctattgaag ctatacatat gactttccca
      421 gagtttagtt atgccgttaa tcattatcag gaaacaattt attttaaacg ttcccagcaa
      481 cttgctcaac tttataacca acatgatatt gatggtttaa aaaccatgtt ggaagacatt
      541 gagggagatg aagtatttga tgtttatgag aagttaaata aattagtcgt taaagtggct
      601 ctctttaatc tggataagag ttatcaagtt cctgatgatg ataaaaaatt attagttaac
      661 tatctatatg ctattgaaga gtggacgact tatgagctct atatatttgg caataccatg
      721 acagctttaa gcaatgaaga tcttatcttt ttaggtaagg cctttatcga gcgggacaag
      781 ctctatagtt ccataccaac tcataaactt ctctcacagc agacacttca aaacttatca
      841 ctagttttat tagaacgacg agaattttac tacacgagat attttatcag ccacttacga
      901 cagttaatta cctttcaaga tatatttact aagattaata tagattttta tgagaaactc
      961 ctcgaccaca tggaaacagg agcaacgaca atggcagatt tagagcagtt tattgacaga
     1021 gtaaaggagc tggcagaccc aatatttcca atgtttttaa gagaaaattt aaggcaaata
     1081 ttagaagtgt aaaatagaaa tgagctatta gaatagctca ttttttctat agaaaaaata
     1141 gtaaaagtgg aacgacaagg ttaataaaga taagtttctt tttcattttt atcatcctcc
     1201 taatactatt gtatatccag aaagtcgaaa tataaaaaag ttgcaaatgt gcaaaaaaat
     1261 aaaaaactaa tttcatattt gggaatatca gaaaaatatg gttttagata gtaaaatggt
     1321 aaaaaacttg gtaaggggga agttgtggaa gcaaggcctg ataatatttt tattaaatta
     1381 agcgataacc caaatattga aaaattgtac gaaaaatttg gtatgtgtct attagaagat
     1441 ataaacgcta gcataaacga gcttgaggtt tatgagatat ggaatagatt tcatcttctt
     1501 agcagtaata ctttttctaa tttgtattta tcatttcttg agccgtattt atttgagata
     1561 agaaatcaat tacagaaata tgatgacagt tttcaaagtg ataccattga acgaactata
     1621 acagagaata tgttagaaaa tatttattct ctatctgaag gtatctggat agcagagatg
     1681 agaaatatta ataaaaatca agctgaatat ttagcttatt taaatcagga ctataaaaat
     1741 ttagcagaag ttgttaaaaa gtatccttac tggtattttt caataatttt atttataaga
     1801 gataccacaa agtatttact agagtttctt tctcatttga ttcaagataa agccttggtt
     1861 gctaatcagt ttttggaagc agatggaatg ataacagata ttaaactatc tatgggagac
     1921 agacatagag ggaaatttgt catcaaggta gaatcagaat tcggtaatta tttttataaa
     1981 ccgagaagaa gtaagttaga tacattattt aaagaagtta tagataaaat aggggatcaa
     2041 gatggaatat taaaaatgaa agctcctaaa attattgatc aaggaagcta ttcttgggct
     2101 gaagaaattc gatatatccc attacaatca aagaataaaa atgagagata ttatattagg
     2161 ttgggacagt tattagctat tgcatatata cttaatggta gcgatttaca ttatgaaaat
     2221 attatatctt gtggagaatt tcctgtagca atagatgttg agacactttt gactagtaga
     2281 ttgaggttca agaagcaaat gactccaaaa atgttacaag aggataatat taactatttg
     2341 gaagactctg taagtaattt gataatatta cctacaattg taaaaagcaa tggagatttt
     2401 tatgatttga gcccgctgaa aatatatagt ggagagttac caaatacttt tgcagaaact
     2461 tggaaaaaat ctaaccatct agtccaaaaa aatgaattaa aagatgtcat agattttata
     2521 cagagaggtt ttactaatat ttataatgag gttctcaaag acaaagagcg ttataaagaa
     2581 tatttcaatc ggctatttaa caatattcct attcgctatt taaatagaga aacccatgat
     2641 tattcgcaaa tattaagact attaaagaat ccagtgtgcc tatttcattt taaatatgct
     2701 tttgcagtat ctatgaaact tctaagtgag gaaatccagg aagaagaagt acaagagtac
     2761 aaggagatgt taaagtttaa catcccatat tttgaattaa atactggtag taccgattta
     2821 cttttatcca atggagagaa gataaaaaat tactttatag aaacacctct agaaagtcta
     2881 gagtataaat tgaatttact atgtgaggaa gatcttgaaa gacaattaag actaataact
     2941 caaatgttga cagtagtttc tacagaattt gacgtaaaag agataaatga agtaacaatt
     3001 catgagggag agaatccaca aaagcaaagg gcgcagacga ttgaacaaat gattgcgact
     3061 gtttttgaaa ataaactgat aaatccatta actaaacaat atttttggat aactcctgaa
     3121 ttagaaggtg ataataatag aagcttttac caaattcatg aaaatcctga tagttattat
     3181 tcgggtagcg ttggaatact aaggtgtttg ctactgctcg aaaaatcagg ctataaagat
     3241 ttacctatta ataaattaat aagtgatatt gaaattgata tagataaaat tattaaatat
     3301 gggacatcag acatcagtat tggtgcttac aacggagtgg ttgaatattt aagatattat
     3361 gcttatatat ataagcataa caactttagt gatgtcttat ctttgaaacg catccattac
     3421 ttattagact taatagaaaa tagctttaaa aaggatgaaa aattagacat cttagacgga
     3481 acggcaggtg tcatattaac tttaatagag atatttcaat tacatttgag tgattgtttg
     3541 aataatcgtg tgattaaatt agttgagaaa tgtcgcttac atctaataga ttcgattatt
     3601 tatgataagg gggaagtcta ttttcctatt gcacaggaaa aaaaacgtta ttttactggt
     3661 tttgcacatg ggtcatctgg aattacttta gctatttaca gagcaaacaa gctattagat
     3721 atagatgact atactcttat tcaatcatta ctaaaaacag aaagacgctt atttgataag
     3781 acaaatcaaa tgtggtttaa ggacaataat agaaacagct attcatgggg atggtgccac
     3841 ggaattccag gtattctttt atcaagactt gagcttataa aacatggcta tcaggatgac
     3901 tatattattg aagaagctta tgaattatat gagattgcta tgcgaaaatc tctgggtgtg
     3961 aatctaactt tttgtcacgg tgatttagga aatcttgtga tttgtaagta tgctgaggcg
     4021 atacttaaca ttgaatacag taaggtagaa atatacattc aagaaattct tccttattta
     4081 ttaaattcaa tggaatataa cattagagga actgaaacgg taggagtaat gcatggctta
     4141 gttgggatag ccactttttt ggacagtacg atttgtgaca actatctcag acttttggat
     4201 attttaaaaa taacatcaga ttaaggaagt ggatatgaaa attttagaga ttcaaaacgt
     4261 aaattttaaa tatggaaaag attatgtctt gaaaaacatt aatcttacgt tagagcaagg
     4321 agatatccta ggtttggttg gtgaaaatgg tgctggaaag tcaactttga tgaagctcat
     4381 ttcaggcatc attcccaact ataaaggaga tattaagatt caagctcata atgttggtac
     4441 tttaattgaa caccccagcc tctatgctga tatgacagtg ttgggtaatt tgaaatttta
     4501 ctgtcgttta tttggcaaga gttacagcgt cattgatgat tataaatatg ttctacaggt
     4561 ggatagttat ttacataaaa aagtttcaaa actatctttg gggatgaagc agagagttgg
     4621 actctttatt gctctcattg cttctgagga gtttatctta ttagatgaac cgacaaatgg
     4681 tctggaccct actggtatta aaaatttact ggacttaata aaaaaattgt catctgaaaa
     4741 gggtattact tttattattt ccagtcacat cttacaaaat ttggagcaaa tttgtaacaa
     4801 agcagttctg ctgagggatc acacaattag cagcttggat gctaaggagc atatgaaata
     4861 taagatttat catcctgatt tatctcaatc tgagctaatc tgcttattag aagataatgg
     4921 tttcgactat gaacaaaatg gtagggatat tattgttcgt gatattgatg ccattgaaga
     4981 aatgttacaa agagagaaga atattactct tgagaaggaa aaaataagtt tgagtgaggt
     5041 atattttgat gaacaataaa tgggcaacgg tcttgtttgt aagactaact attgtgaaat
     5101 ttagtgtatt tatttttctg atgctatccc tattgcttgg tgggggaatc atctattcta
     5161 ttgagaattt aaagggacct tttcaggttt ataatatcta cagtgtattt tctaccgttt
     5221 ccaatttctt attgatgtat gcagctatca atgcctttgg tagagagttt cgctataaaa
     5281 ccatcaatca gcttagaatt agtgggcgat caagtattga aattattttg agaaagttat
     5341 tagcagttga gcttttagct attttgacta gcctagtttc ttttgcggaa gttgcatttt
     5401 ataaaattta ttttaatcat ccgcagattg atttatttga aatttttaac catctggtac
     5461 ctgcttactt agtttatgcg ctatttctct tttcattagg aagcattatc acattagttt
     5521 taaaaagttc attatattca ttcatcactc tattccttac attaagatta ggggtgacta
     5581 tcatgaatgt tatgaataat tttgaatcta cagctgattt gactaaatat attcctttat
     5641 catttgttga aaatgccttt agttttgcta aatacactcc ggaacagtat gtggttacta
     5701 ttgtttggag tgttgcattg atggcattat tacctgtgat ttatcgtaaa tggggttatg
     5761 catagtggaa aacataagaa attcggctta tcccagtata gagggtaaat ttgaaaacta
     5821 tttttctcac tatatttcaa agatatatga gcaattatta tctattgatt caaaaggact
     5881 attcagtagc caaaaagatc aggacaattg ttactacaca cttatcgatg gtatcttaga
     5941 gaatatatca tcagtagcaa ttaattctct gattgactat tatcaggaag aaagagctat
     6001 aaatcctaat ttatcataca aagattataa tgaaaatctt caaaaagata gatattttca
     6061 agaatttcat caaatttatc caacagtgaa agataattgt gaagttgtta tttgtaatta
     6121 tattcaaagg attaaagaag tatttagtct acttcaaaat taccaagtag aaataaggaa
     6181 ctttgctagc ttaaaagaaa gtgagtcgct gtctcttagt aaacttaata cctttgaagg
     6241 tgattttcat attaatacat ttgtagctat acttacacta aatgaaaaaa gaatattgat
     6301 caagaaacgt cctaatttag gggatcgtct tttgagaatt tttcaaaaat attttgataa
     6361 ggaaaatatc aaactaccta ttgcaaatac aattttctta gacgatgact tatgtcttca
     6421 agaatacatc aagtcagata gtgcagtttt aaatagtata gaactccaaa atttttatta
     6481 taaatttgga attataacag ctatttttac aattttagga agtaaggatc ttcatagtga
     6541 gaatattatt tctacaaaat atggtcccta ttttattgat ttagaagctg ctataacgga
     6601 cacactaaat agagaaaaat tagactcact attgacagaa acttatctat ttaattctag
     6661 agaaaaaaga cttgtatatg gttcactaga tttaagtgca tttagtggag gtgatataac
     6721 cacttggcgg catggtgtaa ttcataaggg acaagatgac attaatctag gagtctacag
     6781 ttatactgaa aagcatagta atataccaca agacataaat ggcatcaagg ttaatcctga
     6841 agagtattca gcagatgtac tgaaaggttt cgatacagga attagagttt ttctcaaata
     6901 taaggagagg ttcattcagg aactagaatt aatagagagc tatgattata gggttgtgct
     6961 tcgtaatacg gctttttatg gtaaatattt agatgattta aacttaccaa tgtacactaa
     7021 gagtaaggaa aagtcagaaa attatatcaa cctattgagg aagcacagta aacgcgataa
     7081 aaaaatagtt gacaaagaaa tggcttgttt gaaacaacaa gctatccctt attttttaat
     7141 cagcgaagta actgataata aaataattaa ggacaacctc ataaagagaa ttggagggct
     7201 tagccttaat gatttgaata aagaaaaata ctatttgcag ctaattttgg gattagatat
     7261 tccaatagaa agagaatctc actatttgga taatagaaac agagtggata atgaactaca
     7321 agcatttgaa tcattttgct cgaatgatat gggttttaaa tttggagtga ttgatcacac
     7381 tgttcagaat acgctgttac attattgtaa tgatatatat acatttggtg gttctctctt
     7441 gtttttagct agctattgta aaaatagtaa attgagagaa aaaataatag ctacagtcag
     7501 gagtggtgac acaagtgaag gtctttctgg actaataggt agacaatcgt catacttgct
     7561 ctatcattat tttaatttag aatctgagaa gcaaaactac acatcttcaa aagacatagt
     7621 acatgaaaaa gatattgtag atttttcaac gtatgggagt gccattctgg ttttaaacta
     7681 tctctacaaa gaaacttcaa ataaggctta tctggatgat ataaaactat taggagaaat
     7741 atatttaaaa acttacacta agcgtaatct tactggttta tttcatggat atgcaggcga
     7801 tattttagtg ttatcagttc tccgccaata tatagaacat cagactattg ataaagatat
     7861 tctcgacctt attcaactcg agaattctaa ttttaacaaa gtttctaata attggaatga
     7921 tactaggcac aatatttcta atactgagga tctatgtgca ttatcgtatg gagctgtggg
     7981 aatacttttc agtaggtact tactctatcg ttcatccaat ctttccaaga aattaaaaga
     8041 tattgttata actgatctct ttaaagctct taattgtatt ttggtcagaa aaaggagcga
     8101 ttatatggat gattctctag ttaatggcta tgcaggtgct cttgtggttc taaaaataat
     8161 tttacaatca ggtttattga ataaccacta tagtaaagtg ataaaagtta aaacctatat
     8221 agaagaagga gaaaaattat tagaaaacag ccaatggcgt tatgagcaat ttagtaatct
     8281 tttaaatcct tcatttttta atgggagatt aggtacagta tttactttat ggttcataag
     8341 ccaaaatgat ttttatattg aaaagttctt gggggtgtag gaaattaaaa agaaaaaaat
     8401 tacaccactt ctacagatga gccaaacaga atgtggatta tgtgttgctg taatgttaat
     8461 ggaatactat gatgttcgct taactttgaa tgatattcct aaggaattct ctgcgggaag
     8521 agatgggacg cgtattaggg gacttaagaa aatttttgac cactttggtt tttccatgaa
     8581 tatgtttaag gtgactgata gactctcaga gataaaaaac tcctcgttac caggcatcgc
     8641 ttatagaatc ggaggacatt ttgtcgtagt agaatcaatc tcttctaatg aggttattat
     8701 attggatcca gcttttggac gaacaaaaat ttcaaaggat gaacttgata atgactataa
     8761 aaatttaata cttaagataa cgcctaacga aaattttaaa accgttaata ctagaggaca
     8821 agaattttca ctgattaaag aagcagtatt ctttaacaag aaactcgttg tacaggtgct
     8881 tattgcagcg gtgattgtct attcgttgac actaattgct ccgttactat taaaaggact
     8941 tgttgataat tttttagtta gcaaacagat agatactaca atttgggcac tatctatagg
     9001 tacaatcgca agcagtttgg tctactttgt agtgaataga gtcaagcaaa ttactagcat
     9061 tcgtctgtct atctccgttg ataaattttt atcaaaaaag gttgttgata aattatttaa
     9121 aaataaattt gaattttatc ttaaccgtac tagctcggat atccaatatc gattagtact
     9181 attaaaaaat ttaaaaatga taattagtga tattatcatt caaacttttt tagatatagg
     9241 ttctatggtt gttatcctca tttacattat gaatttacaa cctttatatg cagtcttttt
     9301 agcatttatc acattgctag tattatccat gagcaggttt atgcgagaga aaatgttgtt
     9361 gtacaaaaac aaggagttgg caacggatag taaattgcaa gtttttcagt atgatatttt
     9421 tcgtgcaatt tttgatgtaa aagtgttagg gataagctca aaaaagcata ggtcgtggca
     9481 tagatattat gaggattata tcaagtctca tgaaaaaaat cagcgattta tcactttgta
     9541 tcaaaatata ctagcatata ttacacttta tgtaccaatt ttcattccta tcttaggaat
     9601 ctggattagt caattggatc acagtaacca agttggaacg attatatctt tacaatcgat
     9661 tacaggtaca tacatttcag gtctaatctc catagctcaa ttatcagata atattacaac
     9721 agcaaaatct tacctcatac gtatagatga catccttttg caggaagacg aagaagtaag
     9781 aaatcgtata atagatttta aagggaatat aagggtaaac aacttgtcat ttgcctatcc
     9841 cggtgccgaa gacaaaacat tagatgatat tagtttttca attaatgaag gtgagtctgt
     9901 agccattgtt ggcgaaagtg gttctggtaa gtctacgtta ttttatatat tgttaggtgc
     9961 ttatgataat tatgaaggtg atatatactt tgaaggacta gatttaaaga ttttaaacaa
    10021 agatgcttta cgtgagcaga taggagtagt tcctcaggat gccttattgt ttaacggaag
    10081 tataaaagaa aatttagttg aagattttga ggttgctgat aatgatatat atgacgctct
    10141 aaaaaaagtt actctgcttg attttgttga ttcattgcct atggggatta atacagtggt
    10201 gtcggaaaat gggttcaact tctcaggagg acagagacag aggctagctt tggccagagc
    10261 tattttaaat caaaaatcaa tgctattctt agatgaagca acgagtgctc ttgataatat
    10321 tactgagcat caaattgtag aatatttgtc tgaagattct aaaacaaaga ttgttattgc
    10381 tcatagatta agcactataa aaaatagtga caaaataatt gttttaaaag acggtcaagt
    10441 atctgaagtt ggagaccatc gtagcttgat ggctcgaaag ggagaatatt accgaatgta
    10501 ttctggtaaa taatatattg agcaataact tttgggtagc tgggtgggac aaggagaaga
    10561 taggagtatt atgatgatag gtagtttata aagaatagga aggaatataa aatgaaaagt
    10621 aatttattaa aaataaataa tgtaacagag gtggaaaaag atatggtaac gctcatcaaa
    10681 gatgaagata tggaattagc cgggggctct actccggcat gtgcaattgg ggttgttggt
    10741 attactgtag cagttactgg tatatcaact gcgtgcacat ctcgctgtat taacaaatag
    10801 gagtaaatta taatgaaaga gattcaaaaa gcgggcttgc aagaagagtt atcaattctc
    10861 atggatgatg ctaataactt agaacaactt actgctggta ttggtacaac agtagtaaat
    10921 tctactttta gcattgtatt gggaaataaa ggttatatct gtacggttac tgttgaatgt
    10981 atgagaaact gtcagtagtt ggtactagtg catattaatt ttatagacta tttacatggt
    11041 gactaatatt cctatctgga aagtcttatt taattatatt gatcagtcag tcaatgtggt
    11101 tagtaggact tttttggctc attgtcaact gtagtgggtg acttataact aagcccgaga
    11161 gagaatcaga ttggttctct ctctttgcat gttcaaagcg atgagaattt tagttttaaa
    11221 ggttttgaag tttcgaaagc caaaagctt
//
