LOCUS       BGC0000498             14995 bp    DNA     linear   BCT 19-JUL-2013
DEFINITION  Streptococcus equinus strain HJ50 bovicin biosynthesis gene cluster,
            partial sequence.
ACCESSION   BGC0000498
VERSION     BGC0000498
KEYWORDS    .
SOURCE      Streptococcus equinus (Streptococcus bovis)
  ORGANISM  Streptococcus equinus
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
COMMENT     On Jul 18, 2013 this sequence version replaced EU497962.1.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:44:51
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14995
                     /db_xref="taxon:1335"
                     /mol_type="genomic DNA"
                     /organism="Streptococcus equinus"
                     /strain="HJ50"
     misc_feature    <1..>14995
                     /note="bovicin biosynthesis gene cluster"
     protocluster    1..14995
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3797:6311]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3798..6311
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..14995
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14995
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000498"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14995
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             <1..2728
                     /codon_start=2
                     /product="DNA methylase"
                     /protein_id="ACA51933.1"
                     /transl_table=11
                     /translation="MSNQPTTTKQVVEGNKKKNVTDVEKTTVLRAKETHLQELFQDFVS
                     KYPEVQQMIEDTYNRLYNRTVSKTYDGSYLTIDGLAQNISLRPHQKNAIQRIVEEKRAL
                     LAHEVGSGKTLTMLGAGFKLKELGMVHKPLYVVPSSLTAQFGQEIMKFFPTKKVYVTTK
                     KDFAKAKRKQFVSRIITGDYDAIVIGDSQFEKIPMSREKQVTYVNDKLEQLREIKLGSN
                     SDYTVKEAERSIKGLEHQLEELQKLERDTFIEFENLGIDFLFVDEAHHFKNIRPITGLG
                     NVAGITNTTSKKNVDMEMKVRQVQAEHGDRNVVFATGTPVSNSISELFTMMNYIQPGVL
                     ERYQVSNFDSWVGAFGNIENSMELAPTGDKYQPKKRFKKFVNLPELMRIYKETADIQTA
                     DMLDLPVPEAKIIAVESELTKAQKYYLEELVDRSDAIKSGSVDPSVDNMLKITGEARKL
                     AIDMRLIDPAYTLLDNQKILQVVDNVERIYREGEDDKATQMIFSDIGTPKNKEEGFDVY
                     NELKDLLVDRGIPKEAIAFVHDANTDDKKNSLSRKVNSGEVRILMASTEKGGTGLNVQS
                     RMKAVHHLDVPWRPSDIVQRNGRLIRQGNMHQEVDIYHYITKGSFDNYLWQTQENKLKY
                     ITQIMTSKDPVRSAEDIDEQTMTASDFKALATGNPYLKLKMELENELTVLENQKRAFNR
                     SKDEYRHTISYCEKHLPIMEKRLSQYDKDIAQSLTTKSQDFVMRFDNQSMDNRAEAEDY
                     LRKLITYNRSETKEIWTLANFRGFDLKMTTRAPSEPMPETVSLMIVGDNQYTVALDLKS
                     DVGTVQRITNAIDHILDDQEKTEDMANNLKDKLAVARVEVEKVFPKEEDYQLVKAKYDI
                     LAPLVEQEAEVEEIDAALAKFNETIQPQQDQQLSLDF"
     CDS             2798..3097
                     /codon_start=1
                     /note="orf1"
                     /product="hypothetical protein"
                     /protein_id="AGR45950.1"
                     /transl_table=11
                     /translation="MTQTVEGIRYQLEEWLAQGFTSSEDRANYQALKEQYEDETLDYSF
                     SKREIIGQLEVIITTRENDFPDLDEVTKEEYLDLIAQLDDLDKGKADYYRKQLT"
     CDS             3112..3402
                     /codon_start=1
                     /note="orf2"
                     /product="hypothetical protein"
                     /protein_id="AGR45951.1"
                     /transl_table=11
                     /translation="MLEQIIQSLLIIAATGLILLVLYQIVKMLGSLFVIGLIGFLAFTE
                     VYGLYLFFTERYLYEEDLATNGIWSFTVFYIAFNPLIVAGLVIKVVRNRIV"
     gene            3541..3717
                     /gene="bovA"
     CDS             3541..3717
                     /codon_start=1
                     /gene="bovA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) L_biotic_A"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) leader_eh"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="BovA"
                     /protein_id="ACA51934.1"
                     /sec_met_domain="L_biotic_A (E-value: 9.6e-21, bitscore:
                     63.5, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="leader_eh (E-value: 1.5e-07, bitscore:
                     21.8, seeds: 8, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMNATENQIFVETVSDQELEMLIGGADRGWIKTLTKDCPNVISSI
                     CAGTIITACKNCA"
     CDS_motif       3541..3615
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MMNATENQIFVETVSDQELEMLIGG"
                     /locus_tag="bovA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       3616..3717
                     /prepeptide="core"
                     /locus_tag="bovA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="3.20"
                     /molecular_weight="3359.9"
                     /monoisotopic_mass="3357.7"
                     /core_sequence="ADRGWIKTLTKDCPNVISSICAGTIITACKNCA"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MMNATENQIFVETVSDQELEMLIGG"
                     /leader_location="[3540:3615](+)"
                     /alternative_weights="3378.0"
                     /alternative_weights="3396.0"
     gene            3798..6311
                     /gene="bovM"
     CDS             3798..6311
                     /codon_start=1
                     /gene="bovM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     460; E-value: 2.6e-139)"
                     /gene_kind="biosynthetic"
                     /note="putative lantibiotic modification enzyme"
                     /product="BovM"
                     /protein_id="ACA51935.2"
                     /sec_met_domain="DUF4135 (E-value: 3.1e-58, bitscore:
                     187.7, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 1.4e-29, bitscore:
                     92.9, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEKLDLTINNESESFHKIIDNIVSDFYLVILDAVQTNAELSNVHK
                     YLYKNIRQVLLPILVQDINEWRLESKHQKNDTNQEYIDYCYLFISKNRFAYLKNKYELL
                     NLRIDTIISETKLNLKNFLKNIDKSVSSLKKVFPQCDFEIKKLKFIDFIGDNHGLYQSI
                     MFEVSGKVFFYKCHGSEITNFIVTLQKEIPSLNFLKLPMTYIDQEFIIQEKVTHSSVLI
                     KDDIEEYYHNMGKLLGILYLLNGNDMHNENIIARGKNPIVIDVETLINPIECDSDSKMD
                     DSIFSVGMLPMKYNRNFEGIFDTSSLGQSNIVSEKVFKEYKPFTSEIQLVLIDHTFSDL
                     DRYLPRYKEIHFDVIDYLGVVEDGFIESYHLIMNHKKEIAKVISREASNINCRIVLRNT
                     RIYSEILKFLSTPSLLKNITKAEDTLKRLLVKPRYIIHNWERYRQQEIKQLLNGEIPYF
                     SFGKYGNTSPNDLNLSSVETSLQCKLNRFSDDDLKFQLILLRTSLNIDDDNFEIIGKEN
                     FRGKLKDLIKSSIYGTDIFTLQKDWCGNYIYGESNLGLYEGKLGIILSDSYFSDIFDVS
                     SIRREILFSRDISFIDGRASLDVFDIINNDSILSPVSVEISNKYLDLIEGISGVILVRY
                     KFYCQKLPENISELEQLMRQFMRLYKNSSELKNGFAHGISGIKIIFYIAYQILGEEKYL
                     DEFKKLEILDFPEKYTNGSWCNGLLGWITSEYILYTISGDKTFKENILNHLPRLMTYLI
                     DLSDYCLCHGSFGVLDFLLTLSQKELLPKEFHDEYVKLEKYHLEMITQCRILKKDVSLF
                     TGISGILYYLNRKESGKNSVLTFGF"
     gene            6325..8367
                     /gene="bovT"
     CDS             6325..8367
                     /codon_start=1
                     /gene="bovT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 146.6; E-value:
                     1.7e-44)"
                     /gene_kind="transport"
                     /note="putative lantibiotic transporter"
                     /product="BovT"
                     /protein_id="ACA51936.2"
                     /sec_met_domain="Peptidase_C39 (E-value: 2.5e-34, bitscore:
                     108.0, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRKIKEEIQFQQNDCGPCCLSMILNYYDYQITVPELSSQLSKKNG
                     WSMLDLKTVAAKYYLQSNVFKITDLKGFDKLQCPLVLFWESSHFVVLESISENIVIVDP
                     SIGHVKLNRNEFNQKFSGFAMSFVKNEHFQKRKLSKLRYVKEFNKAEVWSLNFSKKYLL
                     LLLIYNMLLFLAPVVISLFISDLQKSKAYNLIWLALLLFVVAIVVFLLEREKASAVLKL
                     ERDSTYKIYQKIMFQKDSTLSKYHSGDILTRIFSNSEICRFISIDLPNIVSSVILFTTV
                     SLYLVYRIGHLSIFLIFFVLSIGILNGIFVKPLIGLSKKEGYKLSDFRSVSNDGITSHG
                     YLFNSGAIKNYLNKFLNSLSSYIQFSDEKNKFEALATTAQQASNLFFSMLISLLGLSLV
                     ILFPKSSGNVSLLMSSGMILVTPVMSVVSSIVNFAVSYPSFIRLVELINSLEHVSDEQI
                     MENGEIEISNLSFKYPEMKYNIFHNFTVKISQGEQVIITGASGTGKTTLINLILGLEEE
                     YEGEVIVGGVNTKLSNLDLRKDVCYISQPSKLFKGTLKENLELYLDSYHYDELVKLSDK
                     IGLSELMNDISNFENILLLEDGRNFSLGQRQRLTLLRAFLGKYKLVIFDEPTSNLDFSH
                     AKQIFSLIEKIDATKIIITHDKQFLDSSQTIIDLGGQNGYKIMQN"
     gene            8342..9250
                     /gene="bovF"
     CDS             8342..9250
                     /codon_start=1
                     /gene="bovF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 176.4; E-value:
                     1.3e-53)"
                     /gene_kind="transport"
                     /note="putative component of the bovicin HJ50 transporter
                     complex responsible for immunity to bovicin HJ50"
                     /product="BovF"
                     /protein_id="ACA51937.2"
                     /transl_table=11
                     /translation="MDIKLCKINKNYGSRKILKGLNVNVPSGEIYGFIGANGSGKTTTM
                     KIMTGLTPANHGVVKFDGLTLKELAKSEKSFGAFISTPTYYKNLTAVENLTIVQDILGK
                     PKSEVYRVLELVGLSEAADKEVRSFSFGMKQRLGLAFAFLNNPDILILDEPTNGLDPKG
                     IVEIRELLRRLSQEEGKTIFISSHNISEIEAIADRVGIIHQGSLVFEGTLDELYRSNRS
                     TYILEVNDEEKARQLLTEKRIDFMNHQTRFEINLSKSNIPSLLKELIEREISVYEMTPN
                     KNLERIFLNLTAGGTEDAISY"
     gene            9234..9956
                     /gene="bovE"
     CDS             9234..9956
                     /codon_start=1
                     /gene="bovE"
                     /note="putative component of the bovicin HJ50 transporter
                     complex responsible for immunity to bovicin HJ50"
                     /product="BovE"
                     /protein_id="ACA51938.2"
                     /transl_table=11
                     /translation="MLSLIKSEFKKNGNGTQIYLSLAVLLAIQIILMMIIINSFNLTGF
                     GDLKTTEFRKSLLSFAGFSYLFTIFVGAIVGYYFISKEYLNNTWELLILGIKNKFQVVL
                     SKFIVGIIIYIFYQILSLTCFTFLVKTYFGDTIEWSFFMIILVANLSCNLLFYVIQLSA
                     HFLISNSMTAITFSLLLVILPIFLGNNFLFVNFIPLISISYIVNSMAFSMVHFILLSMW
                     TVLVSGFIVIGVSKYFRL"
     gene            9969..10679
                     /gene="bovG"
     CDS             9969..10679
                     /codon_start=1
                     /gene="bovG"
                     /note="putative component of the bovicin HJ50 transporter
                     complex responsible for immunity to bovicin HJ50"
                     /product="BovG"
                     /protein_id="ACA51939.2"
                     /transl_table=11
                     /translation="MKFWRFELYKYRRNMGLAIILFLTTIIVLFQSYTGYITKEIPSKA
                     LYATGLDLLSMLIFPVLIPMVISLSHYIDKENSGLQNLFLKGIDIKQFKLFRWLFYVVG
                     FPILFLIYFGIVLVFIGLRGENIFLTFYNLIPYLLLVVLSIMTLVNISFCIYDLAKQNS
                     LLPILIGLGGAILGTFPFGDHIWLMNPYSYLNYVFSFRRLHTYHIVILIVVTIVTFGGI
                     MILNTSRTECEYEG"
     gene            10669..12180
                     /gene="bovK"
     CDS             10669..12180
                     /codon_start=1
                     /gene="bovK"
                     /note="histidine kinase for bovicin operon expression"
                     /product="BovK"
                     /protein_id="ACA51940.1"
                     /transl_table=11
                     /translation="MKVNSLKISIMYQLVLVIMIIGLGYHHTFNDESSILPLLLEFLFL
                     FIEFILVKIITIKTKFLATCLWTLRLAQIQTISLIIYRGVPLLLQYIIAVLLFNLMLLL
                     VFKLLLQDEKITPVKWTILFGIISSIVSVSFPSFVAPFFVCYMFIVIGILLFYGHKKWN
                     SSVYSHSKNWSYWICMFVFVFNILFLSSVMLFVPGLDLFSGIWQVIVAEFILVLFGILF
                     YNNRLLLSNSNLLLTICLLFLLYWIIFAYIIKRPLQEVLWMELISFALAYIGSLIHHLH
                     LLNGESDNHYNGLSALISEERLKEEFANFLHDDILQDLNAMIQLSRIKNSNEVLPIIND
                     NLERLNVFTRNQMNIYSPQLLKGLSLYENYLQMIESIKKRYPNLPLKIKLSMDSDLKLP
                     PPYDVLVYRWLRELVNNCYKYAQANSLKIDLVIEGAICKISVSDDGIYTSQTTLGEGHG
                     IKTIDSQLRAVGGSLKQYQVMPHGYGILIVFEIEGDRAIESFINR"
     gene            12158..12754
                     /gene="bovR"
     CDS             12158..12754
                     /codon_start=1
                     /gene="bovR"
                     /gene_functions="regulatory (smcogs) SMCOG1016:LuxR family
                     DNA-binding response regulator (Score: 91.6; E-value:
                     6.8e-28)"
                     /gene_kind="regulatory"
                     /note="response regulator for bovicin operon expression"
                     /product="BovR"
                     /protein_id="ACA51941.1"
                     /transl_table=11
                     /translation="MKVLLIDDHTLFSQSLSFALKQFRSDLEIVVINRESDLRKIDDFS
                     IYDVVLLDIHLEKSFSTDGFEIAEYLLNLSSIIKIIMLTGFDLPVYEYKAEQLGIEGFV
                     NKNIEAETLLEVIDSVISGHNYHSKEVEFHDELTDREKEILVFLGEGNKRKEIASKLFI
                     SDRTLTNHIQNILEKLEVDSTIRAIIKARKMGYIK"
     gene            12823..12981
                     /gene="bovI"
     CDS             12823..12981
                     /codon_start=1
                     /gene="bovI"
                     /note="putative lipopeptide for immunity to bovicin HJ50"
                     /product="BovI"
                     /protein_id="AGR45949.1"
                     /transl_table=11
                     /translation="MKKYIPLICFLLFIIFLGITVRAFLADKTLMVADGLLSIVFFISF
                     LITRKKL"
     gene            12992..13477
                     /gene="sdb1"
     CDS             12992..13477
                     /codon_start=1
                     /gene="sdb1"
                     /note="thiol-disulfide isomerase and thioredoxin"
                     /product="Sdb1"
                     /protein_id="ACA51942.1"
                     /transl_table=11
                     /translation="MKKIFKVHLFSLICLVALAIGGNNVYADVNSVNQPSVESATSIEN
                     YNSNIEGLRKIDFATLKSIFDSTSEQSFTIYFGRPTCYYCRLFSPTLKEFNQLLNGGLI
                     YYDTSGQDFDSTAQQFVFDELGIPGTPTIIYVKHGQILNGWVGGGTTAQELMNYLYK"
     CDS             13691..14341
                     /codon_start=1
                     /note="orf3"
                     /product="hypothetical protein"
                     /protein_id="ACA51943.1"
                     /transl_table=11
                     /translation="MALSMLNKMKARLLIGFGGLAVISFIVMMGYTIGSQSVTNHTQKQ
                     IQSEAQKLLTKEKEKEKASVLSDELVKEFLTQYYTKEKLGENNNRIKPYMTDSAFKEEV
                     ARQEDSMNQVYKDYMLDYRFESANIYVNTEANEAFAEVSYQVVYVSDVSDKAQKSTQTE
                     TTSLKLSYAKLSDKLLVNQVTIWKGKLEDLKESSDEVNSSIPKIQGTTTSENS"
     CDS             14381..>14995
                     /codon_start=1
                     /note="orf4"
                     /product="hypothetical protein"
                     /protein_id="AGR45948.1"
                     /transl_table=11
                     /translation="MTRIKAVKQKAILDVAESLGYSFRRLSGHIYEHPDHDSFRIFADT
                     NTFKWFSRDIQGDVIDFVQLVAVVTFKEAVSYLETGDFEQAKLMEETYQPFQYYLHEEP
                     FQQARTYLKDIRGLSNQTINTFGRQGLLAQATYQSESVLVFKSYNHNGTIQAASLQGLV
                     KNEERHKRGYLKKIMKGSHGYVGISFDIGNPKRLIFYESVID"
ORIGIN
        1 gaattccaat caaccaacga ccacaaaaca agttgttgaa gggaataaga aaaagaatgt
       61 gacggatgta gagaaaacaa cagtcctgcg tgccaaggaa acacacctcc aagaactctt
      121 tcaagatttt gtatcaaagt atccagaagt tcaacaaatg attgaagaca cctataatag
      181 gctttacaat cgtacagtat caaagaccta tgatggtagc tatttaacca ttgatggact
      241 tgctcagaat atctccttgc gtcctcacca aaaaaatgcc attcaacgaa ttgtcgagga
      301 aaaacgtgct ctactagccc acgaggtagg gtctggaaaa accttgacca tgcttggggc
      361 aggattcaaa ctgaaagaac tcggaatggt acataaacct ctttatgtgg ttccttctag
      421 tttgactgct cagtttggtc aagaaatcat gaaatttttc ccaaccaaaa aagtctatgt
      481 gaccaccaag aaagattttg ccaaagccaa acgcaagcag tttgtgtctc ggattattac
      541 aggggattat gatgccattg tcattgggga ttcacaattt gaaaaaattc caatgagtcg
      601 tgaaaaacag gtcacctatg tcaatgacaa actcgagcaa ctccgagaga tcaagctagg
      661 aagtaacagt gattacacgg tgaaagaagc ggaacgttcg attaagggat tggaacacca
      721 gttggaagaa ctccaaaaac tagaacgaga tacctttatt gaatttgaaa atcttggcat
      781 tgattttctc tttgtggatg aggctcatca cttcaagaat atccgtccaa tcactggact
      841 tgggaatgtc gcgggaatta ccaacaccac ttcaaaaaag aacgtagaca tggagatgaa
      901 ggtgagacag gttcaggcag agcatggcga tagaaatgtt gtttttgcga caggaacgcc
      961 agtctcaaac tccattagtg aacttttcac catgatgaat tacattcaac ctggtgtttt
     1021 agagcgatac caggtgtcca attttgactc ctgggttggg gcttttggga atattgaaaa
     1081 ctctatggaa ctagccccga caggtgataa gtaccaacct aagaaacggt tcaagaaatt
     1141 tgtcaatttg cctgaactta tgcggattta caaggaaacc gccgatattc agaccgcaga
     1201 tatgcttgac ttacctgtac ctgaagctaa gattattgcg gtggaaagcg agttaacgaa
     1261 agctcagaaa tactatttag aagaactggt tgaccgttca gacgccatta agtcaggcag
     1321 cgttgaccct agtgtcgata atatgttaaa gataactggg gaagctagaa aactagccat
     1381 tgacatgcgt ctcattgacc cagcctatac cttattggac aaccagaaaa tccttcaagt
     1441 agtggataac gtggaacgca tttaccgtga gggagaagat gataaagcta cccagatgat
     1501 tttctcagat attggaacgc ctaagaataa ggaagagggc tttgacgttt acaatgaatt
     1561 gaaagacctc ttagtggata gagggattcc caaagaagca attgcctttg tccatgacgc
     1621 taatacggat gacaagaaaa actctctgtc acgcaaggtc aatagtggag aagttaggat
     1681 tcttatggct tctacggaaa aaggtgggac tggtctaaac gtgcaatcac gcatgaaagc
     1741 ggtccaccac ttagatgttc cctggcgtcc ctctgatatt gtgcagcgaa acggtaggtt
     1801 aattcgtcaa ggcaatatgc accaagaggt agatatttac cattacatta cgaaagggag
     1861 cttcgacaac tacctctggc aaactcagga gaataagctc aaatacatta cgcagataat
     1921 gacctcaaaa gatcctgtga gatcagctga agacattgat gaacaaacta tgacggcttc
     1981 tgactttaag gccttggcaa ctggaaaccc ttatctcaaa ctcaaaatgg agttggaaaa
     2041 tgaactgaca gttttagaga atcaaaaacg agcctttaat cgctccaaag acgagtatcg
     2101 ccataccatt tcctattgcg agaagcacct ccctattatg gaaaaacggt tgagtcaata
     2161 tgataaagat attgcccaat ctttgacaac aaagtcgcaa gattttgtca tgcgatttga
     2221 caatcaatca atggataatc gtgctgaagc tgaggactat ctgcgaaaac tcattaccta
     2281 taaccgctca gaaactaagg aaatttggac tctggccaac tttagaggat ttgatttgaa
     2341 aatgacaaca cgagctccta gtgagcccat gccagagact gtctctttaa tgattgtagg
     2401 agataaccag tatactgtcg cccttgactt gaaatcagat gtgggaaccg ttcaacgcat
     2461 taccaatgcc attgaccata ttctagatga ccaagaaaag acagaagata tggccaataa
     2521 cctcaaagat aagttggcag tcgcaagagt tgaagtggaa aaggtctttc ctaaagaaga
     2581 ggattatcag ctggttaaag ccaaatatga catccttgcc cctttggttg aacaagaagc
     2641 agaggttgag gaaattgatg cagccctagc taagttcaat gaaacgatcc aaccacaaca
     2701 ggaccaacaa ctttcgcttg atttttaaga aaaaggctga caaaaaatag tgaacgcggt
     2761 aaaatgaagt caagaaatca ccaaaggaga attgattatg acccagacag tagaaggtat
     2821 acgttaccaa ttagaagaat ggttagcaca aggttttaca agctccgaag atagagctaa
     2881 ctatcaagcc ttaaaggaac aatatgaaga tgaaaccctt gattatagct tttcaaagcg
     2941 agaaatcatt ggtcaattag aagttattat caccactcgt gagaatgact ttccagactt
     3001 agatgaagta actaaagaag agtatcttga cctaattgcc caactggatg acctagacaa
     3061 ggggaaggca gattattatc gtaagcaatt aacgtagaaa gtggtgtaga tatgttagaa
     3121 caaatcattc aaagtctttt gattatcgca gcaacaggac taattttact tgtcctttac
     3181 cagattgtga agatgcttgg aagcttattt gtcattggat taatcggatt tttagctttc
     3241 acagaagtct atgggcttta cctctttttt acagaacgat acctctatga ggaagattta
     3301 gccaccaatg gtatttggag ttttactgtt ttttatattg cctttaatcc tctgattgta
     3361 gcaggtcttg tgataaaagt cgtgagaaat agaatagtat aaaaataaaa agggctgcct
     3421 gagtgaagtg ccctttttaa aatttgcctt ttttactcat tacaagatct atgcttatat
     3481 tatatactac gtaatgtaaa ggatgtagct atgatattat aaaaatagga ggaaactata
     3541 atgatgaatg ctactgaaaa ccaaattttt gttgagactg tgagtgacca agaattagaa
     3601 atgttaattg gtggtgcaga tcgtggatgg attaagactt taacaaaaga ttgtccaaat
     3661 gtaatttctt caatttgtgc aggtacaatt attacagctt gtaaaaattg tgcttaattt
     3721 attgccgtca aactttttaa aatagctaca agagacctgt tagatatcta ataggtctgt
     3781 cctttttagg agagagtatg gaaaaattag atttaacaat aaacaatgaa tcagaatcat
     3841 ttcataagat aattgataat atagtaagtg atttttattt agtaatttta gatgcagtac
     3901 aaacaaatgc agaactatca aatgtccata aatatttata taaaaatatt agacaggttt
     3961 tgttaccgat tttagtgcaa gatatcaatg aatggagatt ggaaagtaaa catcagaaaa
     4021 atgatacaaa tcaggaatat attgattact gttacctatt tatatcgaaa aatcgatttg
     4081 cttatttaaa aaataaatac gaacttttga atctaagaat tgatactatt atctctgaaa
     4141 ccaagctaaa tttaaaaaat tttttaaaaa atattgataa gagtgtgagt tctttaaaaa
     4201 aagtatttcc acaatgtgat tttgaaatca aaaaattgaa gtttattgat tttatcggtg
     4261 ataatcatgg tttatatcag agtataatgt ttgaagtatc tggaaaggta tttttttata
     4321 agtgtcatgg ttctgaaatc acaaatttta tagtcacatt acagaaagaa attccaagct
     4381 taaatttcct taaactacca atgacatata tagaccagga gtttattatt caagaaaaag
     4441 ttacacattc ttctgttttg ataaaagatg acatagaaga atattaccac aatatgggta
     4501 aattattggg aattctatat ttgctaaatg gaaatgatat gcacaatgaa aatatcatag
     4561 caagaggtaa aaatccaatt gtgattgatg tagaaacact tataaatcca atagaatgtg
     4621 acagtgactc aaaaatggat gatagtatat tttcagtggg tatgctccct atgaaatata
     4681 acagaaattt tgaagggatt tttgatacaa gttcattagg gcaaagtaat attgtttctg
     4741 aaaaagtgtt taaagaatat aaaccattca cttctgaaat ccaattagta ttaatagatc
     4801 atacattttc tgatttggat aggtacttac caaggtacaa agaaatccat tttgatgtga
     4861 tagattattt aggtgttgtt gaagatggtt ttatagaatc ttatcatctt attatgaatc
     4921 ataaaaaaga aatagcaaaa gtaattagtc gggaagcctc caatataaat tgccgaatcg
     4981 ttttaagaaa tactaggatt tatagtgaga ttctcaaatt cttatcgact cctagtcttc
     5041 taaaaaatat tacaaaagca gaagatacct taaaacggtt attagtaaaa ccaagataca
     5101 tcattcataa ttgggaaaga tatagacagc aagaaattaa acagttactt aatggggaaa
     5161 taccttattt ttcatttggt aaatatggaa acacatcacc taatgatcta aatctatcaa
     5221 gtgtagagac ttctttacaa tgtaaattga accgattttc tgatgatgat ttaaaatttc
     5281 agctaatctt gttaagaact tcattgaata ttgatgatga caattttgaa attattggaa
     5341 aggaaaattt tagagggaaa cttaaagacc ttataaaatc atctatctac ggaaccgata
     5401 tttttacatt gcaaaaggat tggtgtggaa attatattta tggggaaagt aatcttgggc
     5461 tttatgaagg aaaacttggc attatcttgt cagatagcta cttctctgat atttttgatg
     5521 taagtagcat tcggagagaa attttatttt cacgagacat tagctttata gatggtcgtg
     5581 ctagtttaga tgtgtttgat ataataaaca atgattcaat tctttctcca gtatctgttg
     5641 aaatatcaaa taaatatttg gatttaattg aaggtatatc cggagtgatt cttgttaggt
     5701 acaagttcta ttgtcaaaaa cttccagaaa atatttcgga attagaacaa ttgatgagac
     5761 aatttatgag attgtacaaa aatagcagtg aattaaagaa tggatttgca cacggtattt
     5821 caggtattaa aataattttt tatattgcgt atcagatatt gggggaagaa aaatatttag
     5881 atgagtttaa aaagcttgag attcttgatt ttccagagaa atatactaat ggatcttggt
     5941 gtaacgggtt actaggctgg attacgtctg agtacattct atatacgata agtggggata
     6001 aaacttttaa agaaaatata ttaaatcatc taccaagatt aatgacttat ctgatagatt
     6061 tgtctgatta ttgtttatgt catgggagct ttggagtatt agatttcttg ttaacattaa
     6121 gccaaaaaga gttattgcct aaggaatttc acgatgaata tgtcaaactt gaaaaatatc
     6181 acttggaaat gattacacag tgtagaatct taaaaaaaga tgtttcactg tttactggca
     6241 tttcaggtat actatattac ttaaatagaa aagaatcggg taagaactca gtactgacgt
     6301 ttggatttta gaggtataat aagaatgaga aaaattaaag aagaaattca atttcagcaa
     6361 aatgactgtg ggccttgttg cttatctatg attttgaatt actatgacta ccaaataact
     6421 gtacctgaac tttcatctca attaagtaaa aaaaatggtt ggagtatgct tgatttaaaa
     6481 acggttgctg ctaaatatta tcttcaaagc aatgtcttta aaattactga cttaaaaggg
     6541 tttgataagc tacagtgtcc tttggtttta ttttgggaaa gctctcattt tgtcgtatta
     6601 gaaagcatct ctgaaaatat tgttatagtt gatcctagta taggacatgt aaaattaaat
     6661 cgtaatgagt ttaaccaaaa attttctggt tttgcaatgt catttgttaa aaatgaacac
     6721 tttcaaaaaa ggaaattaag taaattgagg tacgttaaag aatttaataa agcagaagta
     6781 tggagcttga atttttcgaa aaaatactta ctcttattgt taatttataa tatgctttta
     6841 tttttagctc ctgttgtaat tagtctattt atatctgatc ttcagaaatc aaaagcctac
     6901 aatttaattt ggttagcctt acttttattt gttgtagcta tagtagtttt tcttttagaa
     6961 agggaaaaag cttccgcagt acttaaatta gaaagggatt caacttataa aatttatcag
     7021 aaaattatgt tccaaaagga ttcgacactg tcaaaatatc atagtggcga tattctaaca
     7081 agaatttttt cgaattcaga aatatgtaga tttatttcga ttgatctacc aaatatagtt
     7141 tcatctgtaa tattatttac aacagttagt ttgtacttgg tttatagaat tggtcatctc
     7201 tctatatttt tgatattttt tgtattaagt ataggaatat tgaatggtat atttgttaag
     7261 cctttgattg gcttatctaa gaaggaagga tataaactgt cagattttag atctgtatcg
     7321 aatgatggaa ttacatcgca tggatattta tttaattctg gagccataaa aaattatcta
     7381 aataaatttt taaattcttt atcaagttat atacaatttt cagacgaaaa aaataagttt
     7441 gaagctttag ccacaactgc tcaacaagcg tcgaatctat ttttttcaat gttaatctca
     7501 ttacttggac taagtttagt aattttattt ccaaaaagta gtggaaatgt ctctttgtta
     7561 atgtcatctg gtatgatatt agtaactcca gtcatgtctg tggtatctag tatagtaaat
     7621 tttgcagtgt cttatccaag ttttataaga ttggttgaat taataaatag tttagaacat
     7681 gtttcagatg agcagataat ggaaaatgga gaaattgaaa tatcgaattt atcttttaaa
     7741 taccctgaaa tgaaatataa catttttcat aattttacag taaagatttc tcaaggggaa
     7801 caggtgatta ttactggtgc ttcaggaact ggaaagacga ctttaattaa tcttatttta
     7861 ggacttgagg aggaatatga aggcgaggta attgtaggag gagtaaatac taaattaagt
     7921 aatttagatt tacgaaaaga tgtctgttat atttcccagc catcaaaatt atttaaagga
     7981 actttgaagg aaaatttaga gttgtatctg gattcttatc attacgatga acttgttaag
     8041 ttatcagata aaattggtct atcagaattg atgaatgata tatcaaattt tgaaaatatt
     8101 ttacttttag aagatggaag aaatttttct ttaggtcaaa ggcagagatt aacgctgctt
     8161 agagcgtttt tagggaaata taagctggtt atttttgatg aaccgactag taatttagat
     8221 ttcagtcatg cgaaacaaat attttcactt attgagaaaa ttgatgcaac aaaaattatt
     8281 attactcatg acaaacagtt tttagattcg tctcaaacta tcatagattt aggagggcaa
     8341 aatggatata aaattatgca aaattaataa aaattatgga agccgtaaga ttttaaaagg
     8401 actaaacgta aatgttcctt caggtgaaat ttatgggttt ataggtgcta atggttcagg
     8461 gaaaacaact actatgaaaa ttatgactgg attaactcca gcaaatcatg gagttgtcaa
     8521 atttgacgga ttaacattaa aagaattagc taaatcggaa aaaagttttg gagcctttat
     8581 tagtacacca acctactata aaaatttaac ggcagttgaa aatctgacta tagtacaaga
     8641 tattcttgga aaacctaaaa gtgaagtata cagggttcta gaattggttg gattatctga
     8701 agctgcagat aaagaagttc gctcattttc ttttggaatg aagcaacgtt taggattggc
     8761 ttttgctttt ctaaataatc ctgatatctt gattttagac gagccgacaa acggtttaga
     8821 tcctaaaggg attgtcgaaa tccgtgaatt acttcgaaga ttatcacaag aagaaggaaa
     8881 aactatcttt atttctagtc acaatatttc tgaaatagaa gctattgctg acagggtagg
     8941 aattattcat caggggagcc ttgtctttga agggacttta gatgagttgt atagaagtaa
     9001 tcgcagtacg tacattttgg aggtaaatga tgaagagaaa gctcgacaac tcttaacaga
     9061 aaaaagaatt gattttatga atcatcaaac aaggtttgag attaatttat ctaaaagtaa
     9121 tattccctct ttactgaagg agttgattga gagagagatt tctgtttatg aaatgacccc
     9181 aaataaaaat cttgagagaa tctttttaaa tctgactgca ggaggtactg aagatgctat
     9241 ctcttattaa atcagaattt aaaaagaatg gaaatggcac acaaatatat ttatcacttg
     9301 cagtgctact agcgatacaa attatcttga tgatgattat tataaactcc tttaatctta
     9361 ctggatttgg agatttaaaa actacagaat ttagaaaaag tttactaagt tttgcaggat
     9421 tttcgtatct ttttactata tttgtgggag cgatagttgg gtattatttt atttctaaag
     9481 aatatctcaa taatacttgg gaattattga ttttggggat aaaaaataaa tttcaagtag
     9541 tcctatcaaa atttatagta ggtattatta tttatatttt ttatcaaatt ttatccctta
     9601 cctgttttac gtttcttgtt aaaacttact ttggtgatac tattgaatgg tcatttttta
     9661 tgataatctt agtggccaac ctatcttgta atctgttatt ttatgtgatt caattatctg
     9721 cccatttttt gatttcaaat agtatgacgg ctataacttt ttcattgtta ttagtaattc
     9781 tgccaatttt tttaggaaat aatttcttat ttgtcaattt tattcctctc attagtatct
     9841 cctatattgt gaatagtatg gcattttcaa tggttcactt tatcttactc tctatgtgga
     9901 ctgttttagt aagtggtttt atagttattg gtgttagtaa gtattttagg ttataaggga
     9961 ggatgaaaat gaagttttgg agatttgagt tgtataaata tcggcgaaat atgggacttg
    10021 ctattattct atttctcaca acaataatag ttctattcca atcttatact ggatatatca
    10081 ctaaggaaat tccttcaaaa gctttatatg ctacaggtct agatctgtta tcaatgctga
    10141 tattcccagt gttaattcct atggtcattt ctttatccca ttatatagac aaagaaaatt
    10201 ctgggcttca aaatttattt ttgaaaggaa tagatataaa acaatttaaa ctatttcgat
    10261 ggcttttcta tgtagtcggc tttccgatat tatttttaat ttattttggt atagtattgg
    10321 tgtttattgg tttgcgaggc gaaaatatct ttctcacttt ttataatctg ataccttatt
    10381 tattacttgt tgttctatct atcatgacat tggtaaatat cagtttttgt atatatgact
    10441 tagcaaagca aaatagttta ctacctatac taattggtct tggaggagct atcctaggaa
    10501 cattcccatt tggggaccat atttggttaa tgaatcctta ttcttactta aattatgtct
    10561 tctcatttcg tcgtttacat acctatcata tcgtaatctt gattgtagta accatagtaa
    10621 catttggtgg tataatgata ctaaatacct caagaacgga atgtgaatat gaaggttaat
    10681 agtttaaaaa taagtataat gtatcagctt gtattagtca taatgatcat cgggctagga
    10741 tatcatcata cttttaatga cgagagttca attcttccat tattgttaga gtttctattt
    10801 ttgtttattg aatttatttt agtaaaaatt attacaataa agacaaagtt tcttgcaact
    10861 tgtctttgga cattaaggtt agcgcaaatc cagactattt ccttaataat ctacagagga
    10921 gtcccacttt tgctacagta tatcattgct gttttgttat ttaatctaat gttgctacta
    10981 gtttttaaat tattgttgca agacgaaaaa ataactcctg taaaatggac aatattattt
    11041 ggaataatat caagtattgt ttcagtttct tttccatctt ttgttgctcc tttttttgta
    11101 tgttatatgt ttattgttat tgggatactt cttttttatg gtcataaaaa gtggaacagt
    11161 tccgtttatt cacatagtaa aaactggagc tactggattt gtatgtttgt atttgtattc
    11221 aatatattgt ttcttagttc ggtaatgctg tttgtaccag gactagatct tttctctggt
    11281 atctggcaag ttattgtggc agagtttatt ctagttttat ttggaatttt attttataac
    11341 aatcgattgt tgcttagtaa tagtaatcta ttattaacaa tttgtctgct atttttactc
    11401 tattggatta tttttgcata tatcataaag agacctttac aagaggtatt gtggatggag
    11461 ttgatttcct ttgcgcttgc ttatatcggg agtttaattc accatctcca tctgttaaat
    11521 ggagaaagtg ataatcacta taatggcctt tctgccttga ttagtgaaga aagattaaaa
    11581 gaagagttcg caaattttct tcatgatgat attttacaag atttaaatgc gatgattcag
    11641 ctaagtagga taaaaaattc aaatgaggta ttaccaatta ttaatgataa cttagagaga
    11701 ctgaacgtgt ttacgagaaa ccaaatgaat atatatagtc cacaactatt gaaaggacta
    11761 agtttatatg aaaattatct tcaaatgatt gaatcaataa aaaaacgtta tccgaatctt
    11821 ccattaaaaa ttaagttgag tatggattca gatttaaaac ttcctcctcc gtatgatgtc
    11881 ttggtttatc gttggcttag agaattggtt aataattgtt ataaatacgc tcaagctaat
    11941 tcattaaaga tagatctagt aattgaagga gcaatctgta aaatttcggt ttccgatgat
    12001 ggaatatata cttcacaaac tactttgggt gagggacatg gtattaagac aatagactca
    12061 caattacgag cagttggtgg aagtttgaag cagtatcaag tgatgcccca tggatatggg
    12121 atactaattg tatttgaaat tgaaggagat agagcaattg aaagttttat taatagatga
    12181 tcatacatta ttttcacaga gtttatcatt tgctttaaaa caatttagga gtgatttgga
    12241 gatagtcgta ataaatagag aatcagattt gagaaaaatt gatgattttt cgatttatga
    12301 tgttgttttg ttagatattc acttagaaaa gtctttttct actgatgggt ttgagatagc
    12361 agagtatctg ttaaatttat cttctattat aaagattatc atgttgacag gttttgactt
    12421 gccagtttac gaatataaag cagaacaatt agggatagaa ggttttgtca ataaaaacat
    12481 agaggcagag actctcttag aggtaattga ttcagtgatt tcaggtcaca actatcattc
    12541 aaaagaagtt gagtttcatg acgaattaac agatagagaa aaggaaatct tagttttttt
    12601 aggtgaaggt aataaacgta aggaaatagc tagtaagtta tttatttcag atagaacact
    12661 aacgaatcat attcaaaata ttttagagaa actagaagtt gattcaacaa tcagagctat
    12721 aataaaggct cgaaaaatgg gttatattaa ataatttcct tttttactca tgaaaaaaat
    12781 attgtattac catataataa agatgaagga ggaattatct agatgaaaaa atatattcca
    12841 ctaatttgtt tcttgctgtt catcatattt ttggggatta ccgtaagggc atttttagct
    12901 gataaaacat tgatggtagc ggatggactg ctttctattg tattttttat ttcattttta
    12961 atcacaagaa aaaaattgta aaggagtata catgaaaaag atttttaagg ttcatttgtt
    13021 ttcgctaatc tgtttagtag ctttagcgat aggtggaaat aatgtgtatg ctgatgtgaa
    13081 ttccgtaaat cagccaagtg ttgagtcagc gacatctatc gaaaattata attcaaatat
    13141 tgaaggttta aggaagatag attttgctac tttaaaaagt attttcgata gtactagtga
    13201 acaatctttt actatctact ttggtcgacc aacatgctat tactgtagat tattttctcc
    13261 tacactaaaa gaatttaatc aattattgaa tggtggatta atatactatg atacaagtgg
    13321 acaagacttt gattctactg cgcaacaatt tgtttttgac gaattgggca ttccaggaac
    13381 acctactatt atttatgtca agcatggaca aattctaaat ggatgggtag gaggaggtac
    13441 taccgctcag gaactaatga attatctgta taaataaatg atttattggt attagaagaa
    13501 cgatttaata attttacata atttatcttc taataacttt tactcatatt gaagacaaat
    13561 gctcaagaat agagtgtttg tcttttcatt ttactgttat ttgatgtaag atgatatgat
    13621 tgcatcattt ttgtgataat ttttccaagt caatcttgtt atgatggggc tatcttatta
    13681 aaaaaaggag atggcactgt ctatgttaaa taaaatgaaa gctaggcttc taattggttt
    13741 cggtggttta gctgtgatta gctttatcgt gatgatgggt tatactattg gttctcagtc
    13801 tgtcaccaat cacacgcaga aacaaatcca gtctgaggct caaaaattac tgaccaaaga
    13861 gaaagaaaaa gagaaggcga gtgttctctc tgatgagcta gtcaaggaat tcctcaccca
    13921 atactatact aaggaaaaac tgggcgagaa caacaaccgt atcaagccct atatgacaga
    13981 ttctgccttt aaagaagagg tagctcgtca agaagattcc atgaaccagg tctataaaga
    14041 ctatatgttg gattatcgct ttgagtctgc caatatctat gtcaatacag aagcaaatga
    14101 agcttttgcg gaagtgtctt atcaggttgt ttacgtgtca gatgtcagtg acaaggctca
    14161 gaaaagtaca cagacagaaa cgacctctct taaactatct tatgccaaat tgtcagataa
    14221 actccttgtc aatcaggtaa ccatttggaa aggcaagcta gaggacttga aagagtcttc
    14281 tgatgaagta aattcaagta ttccaaagat ccaaggaact acaacaagtg agaacagcta
    14341 gcaggagaca gtcttctgct ttttttgata gggagacaaa atgacacgaa ttaaagcagt
    14401 aaaacaaaag gccattttag atgtggctga aagtctgggg tattccttta gacgtttatc
    14461 aggacacatt tatgaacacc cagaccatga ttcctttcgg atttttgccg ataccaatac
    14521 tttcaaatgg ttttcaagag atatacaagg ggatgtaatt gactttgttc aattagtggc
    14581 agtcgttact ttcaaagagg ctgtatccta tcttgaaact ggggattttg aacaagctaa
    14641 gttgatggaa gaaacgtatc aaccgtttca atattatttg catgaagaac cctttcagca
    14701 agcacgtact tacttgaaag acatccgtgg actaagtaat cagactatca atacctttgg
    14761 cagacaagga ttgcttgctc aagctaccta tcaatctgag tcagttctgg tgtttaaaag
    14821 ctataaccat aatgggacta tacaggcagc aagtcttcaa ggtctcgtca aaaatgaaga
    14881 aagacacaaa cgtggttatt tgaagaaaat catgaaaggc tctcatggct atgtcggtat
    14941 tagtttcgat attggtaatc ccaagcgact cattttttat gaatcagtta tcgat
//
