LOCUS       BGC0000500              8863 bp    DNA     linear   BCT 27-JAN-2014
DEFINITION  Carnobacterium maltaromaticum strain C2 CrnM (crnM), CrnA1 (crnA1),
            CrnJ (crnJ), CrnT (crnT), CrnP (crnP), CrnA2 (crnA2), and CrnY
            (crnY) genes, complete cds.
ACCESSION   BGC0000500
VERSION     BGC0000500
KEYWORDS    .
SOURCE      Carnobacterium maltaromaticum
  ORGANISM  Carnobacterium maltaromaticum
            Bacteria; Bacillota; Bacilli; Lactobacillales; Carnobacteriaceae;
            Carnobacterium.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:44:55
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8863
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            1..3012
                     /gene="crnM"
     CDS             1..3012
                     /codon_start=1
                     /gene="crnM"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     678.7; E-value: 2.4e-205)"
                     /gene_kind="biosynthetic"
                     /note="lantibiotic synthetase"
                     /product="CrnM"
                     /protein_id="AHF21237.1"
                     /sec_met_domain="DUF4135 (E-value: 6.5e-94, bitscore:
                     304.0, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 4.9e-38, bitscore:
                     119.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDINKNKTKTINEKIKIFTKEEVIDISYFEEWRSVRTLLNENYF
                     KIMLEEMNISKNQFSYALQPLNDEFKLHTNVKNEEWIKCFNRVINNFNYKNINYKVGLY
                     LPIQPFSVYLQEKLKEILKKLNNIKINDKIIDAFIEAHLIEMFDLVGKVIALKFEDYKQ
                     INFLKNTNNGTRLEEFLRSTFYSRKSFLKLFNEFPVLARVCTVRTIYLINNFSAIIQNI
                     NSDYLEIQEFLNVDFLNLTNITLSTGDSHEQGKSVSILYFDEKKLIYKPKNLKISEIFE
                     SFIDWYTNVSNHKLLDLKIPKGIFKDDYTYNEFIEPNYCENKREIENYYNRYGYLIAIC
                     YLFNLNDLHVENVIAHGEYPVIVDIETSFQVPVQMEDDTLYVKLLRELELESVSSSFLL
                     PTNLSFGMDDKVDLSALSGTMVELNQQILAPVNINMDNFHYEKSPSYFPGGNNIPKNNK
                     SVTVDYKKYLLNIVTGFDEFMKYTQENQLEFIEFLKKFSDKKIRVLVKGTEKYASMIRY
                     SNHPNYNKEMKYRERLMMNLWAYPYKDKRIVNSEVQDLLFNDIPIFYSFPNSRDLIDSR
                     GLVYKDYLPVTGLQKAIDRVKDTSVKSLFDQKLILQSSLGLWDEILNKPVQKKELLFEK
                     QNFNYVKEAINIAELLIGYLIETDDQSTMLSIDCSEDKHWKIVPLDESLYGGLSGIALF
                     FLDIYKITKDEKYFNYYDKIISTAIKQCKATIFSSSFTGWLSPIYPLILEKKYFGTMKD
                     KKFFDYTMEKLSNMTEEQINNMDGMDYISGKAGIVKLLISAYRESKNNENIGLALSKFS
                     NDLIQNIGTGKVSELQNVGLAHGISGIMVVVASLDTFKSEYIREQLAIEYEMFCLREDS
                     YKWCWGISGMIQARLEILKLSPECVDKKELNLLIKRFKNILNQMINEDSLCHGNGSIIT
                     TMKMIYMYTQDTEWNSLINLWLSNVSIYSTLQGYSIPKLGDVTIKGLFDGICGIGWLYL
                     YSNFSIENVLLLEV"
     source          1..8863
                     /db_xref="taxon:2751"
                     /isolation_source="Surubim"
                     /mol_type="genomic DNA"
                     /organism="Carnobacterium maltaromaticum"
                     /strain="C2"
     protocluster    1..8863
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[0:3012]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..3012
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..8863
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8863
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000500"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8863
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            3127..3348
                     /gene="crnA1"
     CDS             3127..3348
                     /codon_start=1
                     /gene="crnA1"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="lantibiotic carnolysin A1 precursor peptide"
                     /product="CrnA1"
                     /protein_id="AHF21238.1"
                     /transl_table=11
                     /translation="MSELSMEKVVGETFEDLSIAEMTMVQGSGDINGEFTTSPACVYSV
                     MVVSKASSAKCAAGASAVSGAILSAIRC"
     CDS_motif       3127..3210
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MSELSMEKVVGETFEDLSIAEMTMVQGS"
                     /locus_tag="crnA1_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       3211..3348
                     /prepeptide="core"
                     /locus_tag="crnA1_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-0.60"
                     /molecular_weight="4171.8"
                     /monoisotopic_mass="4169.1"
                     /core_sequence="GDINGEFTTSPACVYSVMVVSKASSAKCAAGASAVSGAILSAI
                     RC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSELSMEKVVGETFEDLSIAEMTMVQGS"
                     /leader_location="[3126:3210](+)"
                     /alternative_weights="4189.8"
                     /alternative_weights="4207.8"
                     /alternative_weights="4225.8"
                     /alternative_weights="4243.8"
                     /alternative_weights="4261.9"
                     /alternative_weights="4279.9"
                     /alternative_weights="4297.9"
     gene            3501..4223
                     /gene="crnJ"
     CDS             3501..4223
                     /codon_start=1
                     /gene="crnJ"
                     /note="lantibiotic reductase"
                     /product="CrnJ"
                     /protein_id="AHF21239.1"
                     /transl_table=11
                     /translation="MKKVFVLIGSRKKNGNTASFIKSVTDRLEDEHYDVEFAFPQDYDI
                     NFCDGSNDIFIDTDYSLNDELEILQNKILESDIFIIGSPVYVHSMSADLKLFIERSAWW
                     VHTLRLQGKPVIVMSTCGSNGLKTVIEPLSEVITFMGGNVIATANATQIPDRLNDKVWI
                     KEISEEIITRINTYSELPPMSNKFLEKVFNGSKLNILEQLKLEDKVNTKFGELVYWQKT
                     GMINFDNFSSYLEYIQRK"
     gene            4233..6323
                     /gene="crnT"
     CDS             4233..6323
                     /codon_start=1
                     /gene="crnT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 262.5; E-value:
                     1.4e-79)"
                     /gene_kind="transport"
                     /note="lantibiotic transporter and protease"
                     /product="CrnT"
                     /protein_id="AHF21240.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 5.9e-36, bitscore:
                     112.2, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIRFQQQSEHSECGLACATMLIDYWSEKVNLTEMRERYGVPNGG
                     YNLAQIQLILRDADIDVKAVKSEAKSINAVPVPFIAFWKNKHFIIVEKVTHKHIVVVDP
                     ARGKRKISHLEFEDGYSNICMYITTKRTRKYQQPKLNPVLKELVKNNKKNFIKTFFIFL
                     VIQCISIYIPYAIRWIIDGEEKDMRKIIFYASFLISSYFIANVFRTKLIVLLQTSTDKD
                     LLSRTIIQLLDLPYSYFVNRSKGELIYRINSNAYIRQILIDQVINTVIDLFFFAFYLIV
                     MWTFNKTLTIITIFIAIVTIFFSYLNTKIMKKIAQNEMVVLTNSQELINEMVNNIFTIK
                     STNSQKNMYEKWEKNYDEQIIYEKEKAKYNSFLANIPQTIQTFYSLIIYAVGYFLIINS
                     MTTIGNIVAFSTIGISFLTPITSILNSYTQFQTVKVYLDRLFDILETKSESSLLGNEEL
                     KDYSGDVTIKDVMYKYSSFSEEAIKNISLTIKPRQKVAIVGASGSGKSTLLKVVAGLYQ
                     SSKGNIYYGNQNIKDLDIHKLRDSIGVVLQENVLFNGTFRENITMGRNHTDKEITEIIK
                     AVGLSELVSSFPLALETQISELGQNMSGGQRQKVSIARTIITKPKIIFLDEPTSALDNI
                     SENVVMNCLFNIDTTLIVVAHRLSTIQNFDKIVVMDKGEVVGVGNHAELLNTNKYYQNL
                     YQ"
     gene            6388..7752
                     /gene="crnP"
     CDS             6388..7752
                     /codon_start=1
                     /gene="crnP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 206.1; E-value: 2e-62)"
                     /gene_kind="biosynthetic-additional"
                     /note="lantibiotic protease"
                     /product="CrnP"
                     /protein_id="AHF21241.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 8e-35, bitscore:
                     109.3, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRILVKNILSIISSVLIFVVLSGNRVVAFAQEPSIDLLVSEGESI
                     EQLKEELTEVDNKLEFIEIPEINLLRIENPNEKVQEVIENSDEIDFSGKISDKLILENN
                     TITEKNVDFPKIFQYSAEEQNIETELLNRLTWYINTLTQDKKAFEYSKGKNIKIGLIDS
                     GVDTSHPLISPSLNLEKAKSFVKNDQSIEDSNGHGTMVAGVISQVSPEAKITPYRVMSA
                     IDGESIWTLQAIIRAVNDKQDIINMSLGTYKYETKKDERITIEAFKRAACYAFIKKVLI
                     ISSSGNQQLDSDVNYRENKIMHLPGDIKGVITVSAINKNNQLTNYSNTGTNVQYTAPGG
                     EIIIDENGYLDARELIYTMYPLTLPNPMKDAGIPDGYTLTYGTSFSSAGVTAVFANYYS
                     YYLQIMQKKPNSQNAHKFIAYNSTDFGVVGKDSQYGYGLPNLIRAYELISSNKNH"
     gene            7880..8059
                     /gene="crnA2"
     CDS             7880..8059
                     /codon_start=1
                     /gene="crnA2"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="lantibiotic carnolysin A2 precursor peptide"
                     /product="CrnA2"
                     /protein_id="AHF21242.1"
                     /transl_table=11
                     /translation="MSESNMKKVVGETFEDLSIAEMTKVQGSGDVMPESTPICAGFATL
                     MSSIGLVKTIKGKC"
     CDS_motif       7880..7963
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MSESNMKKVVGETFEDLSIAEMTKVQGS"
                     /locus_tag="crnA2_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       7964..8059
                     /prepeptide="core"
                     /locus_tag="crnA2_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="-1.50"
                     /molecular_weight="3047.7"
                     /monoisotopic_mass="3045.6"
                     /core_sequence="GDVMPESTPICAGFATLMSSIGLVKTIKGKC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSESNMKKVVGETFEDLSIAEMTKVQGS"
                     /leader_location="[7879:7963](+)"
                     /alternative_weights="3065.7"
                     /alternative_weights="3083.7"
                     /alternative_weights="3101.7"
                     /alternative_weights="3119.7"
     gene            8495..8863
                     /gene="crnY"
     CDS             8495..8863
                     /codon_start=1
                     /gene="crnY"
                     /note="transcriptional regulator"
                     /product="CrnY"
                     /protein_id="AHF21243.1"
                     /transl_table=11
                     /translation="MKKKVSSSKMDKYTIIMISFILLSMLSIGPSLYLPSYWAFLPPIV
                     LWLVGMYGAIRVEKMKKTENIRTYKEIINFMENKDSIKDNNQDSKSKDRLLLIITVILF
                     SLIGGGIALGSIWLINIL"
ORIGIN
        1 atgaatgaca ttaataagaa taaaacgaaa actattaatg aaaaaattaa aatatttact
       61 aaagaagaag tcatagatat tagttatttt gaagagtgga gaagtgttag gacattatta
      121 aatgaaaatt actttaaaat tatgttggaa gaaatgaaca tttcaaaaaa tcagttttca
      181 tatgcacttc agccattgaa tgatgaattt aaacttcata ctaatgttaa aaatgaagaa
      241 tggataaaat gttttaaccg agtaattaat aattttaatt ataaaaatat aaattataaa
      301 gtaggtctat atttacctat tcaaccattt agtgtatatt tacaagaaaa attaaaagaa
      361 atattaaaaa aattaaataa tataaaaatt aacgataaaa ttatagatgc atttattgaa
      421 gctcatttaa tagagatgtt tgatttagtc ggaaaagtta ttgctttaaa atttgaagac
      481 tacaagcaaa ttaatttttt gaaaaatact aataatggta cgaggcttga agaatttttg
      541 cgtagtacat tttattctag aaaatctttt ttaaaattat ttaatgagtt tccagtatta
      601 gcaagagttt gtactgttag gactatttat ttaataaata atttctcagc tattattcaa
      661 aatattaatt ctgattactt agaaatacag gagtttttga atgttgattt tttaaattta
      721 acaaatataa cattatctac tggtgattct catgaacaag gaaaatcagt atctattttg
      781 tattttgacg aaaaaaagtt aatttataaa cccaaaaatt taaaaataag tgaaatattt
      841 gaatctttca ttgattggta tactaatgtt agtaaccata aactattaga tttaaaaatt
      901 ccaaaaggaa tttttaaaga tgattatact tacaatgaat ttattgagcc gaattattgt
      961 gaaaataaaa gagaaataga aaattattat aatagatacg ggtatttaat tgctatttgc
     1021 tacttattta atctaaatga tttgcatgta gaaaatgtta ttgctcatgg agagtaccca
     1081 gtaatagttg atatagaaac atcatttcag gttcctgttc aaatggaaga tgatactctt
     1141 tacgtaaaat tgttacgtga attagaatta gaatcagtat ctagttcatt tttactgcca
     1201 acaaatttaa gttttggaat ggatgataaa gtcgatttaa gtgcactcag tggaacaatg
     1261 gttgaactta atcagcaaat acttgcacca gtaaatatca acatggataa ttttcattat
     1321 gaaaagagtc ctagttattt tcctggtggg aataatatac ctaaaaataa caaatcagtt
     1381 actgtggatt acaaaaaata tctcctgaat attgtaactg gttttgatga atttatgaaa
     1441 tacactcaag aaaatcaatt agaatttatt gaatttttaa agaaattttc tgataaaaaa
     1501 attcgtgtac ttgttaaagg aaccgaaaag tatgcttcaa tgattaggta tagtaatcat
     1561 ccaaattata ataaagaaat gaagtataga gaacgtttaa tgatgaatct ttgggcttat
     1621 ccatataaag ataaaagaat tgttaacagt gaagtgcaag atttattatt taatgatatt
     1681 cctatttttt actcattccc taattccaga gacttgattg atagcagagg attagtctat
     1741 aaagactatt taccggtaac aggccttcaa aaagcgattg atagagtaaa agatacctct
     1801 gttaaaagtt tatttgatca aaaattaatt ttgcaatcat ctttaggatt atgggatgaa
     1861 attcttaata agccagttca aaaaaaagag ttactttttg aaaaacaaaa tttcaattat
     1921 gtaaaggaag ccattaatat tgctgaacta ctaatagggt atttaattga gacggatgat
     1981 caatccacaa tgttaagcat tgattgctct gaagataaac attggaaaat agtaccttta
     2041 gatgaaagtt tatatggagg tctaagtggc attgcattgt tctttctaga tatttataag
     2101 ataaccaaag atgaaaaata ttttaactat tacgataaaa taattagtac agcaataaag
     2161 caatgtaaag ctactatttt ttctagctca tttacaggtt ggttatcacc aatatatccg
     2221 ttaattttag aaaaaaaata ttttggaacg atgaaagata agaagttttt tgattataca
     2281 atggaaaagc tatcaaatat gactgaagag caaataaata atatggatgg aatggactat
     2341 atatctggta aagctggaat agtaaaatta ctaatatcag cttatcggga atctaaaaat
     2401 aatgaaaata taggccttgc tttaagcaaa ttttcaaatg acttgattca aaatataggg
     2461 acaggaaaag tatcggaatt gcaaaacgtt gggttagcac atggaataag tgggattatg
     2521 gtcgtagttg catctttgga tacatttaaa tcagaatata tcagagaaca acttgctata
     2581 gaatatgaaa tgttttgttt acgagaagat tcttataaat ggtgttgggg gatatctggc
     2641 atgattcaag ctaggttaga aattttaaaa ttatcgccgg aatgtgttga caaaaaagaa
     2701 ctaaatttat taattaagag gtttaagaac attttgaatc agatgattaa cgaagatagt
     2761 ttatgtcatg gaaatggcag tataataacg actatgaaaa tgatttatat gtatacacaa
     2821 gatactgaat ggaattcttt gattaattta tggttatcta atgtatctat atattcaaca
     2881 ttacaaggat attcaatccc taaattgggt gatgtaacta taaaaggttt atttgatggt
     2941 atttgcggta ttggatggtt atatctttat tcaaattttt ccatagagaa tgttttgttg
     3001 ttagaagtct aattgagact agcacaattt tgattagctt ttatataaaa atatttcaaa
     3061 gggaggtagg ttaattaatt agtacaagta tagttgtgca aaaaaatata ttggaggaaa
     3121 attattatgt cagaattaag tatggaaaaa gtagttggag aaacttttga agatttatcg
     3181 attgctgaga tgactatggt acaaggatct ggagatatta atggtgaatt tactacatca
     3241 ccagcctgtg tttatagtgt aatggttgtt tccaaggctt catcagctaa atgtgcagct
     3301 ggcgcttcag cagtcagcgg tgcaatactt tcagctatta gatgttaaat aataatggtt
     3361 tggtataata agtgatttta cgtaaaaatt ataattttac tttgcatata gaatatagta
     3421 tttgcctata tgcaaagtat tttatatatt tataaaaagt attcattaaa ccagataact
     3481 taagtagggg gatgctgact atgaaaaaag tatttgtact tatcggaagt agaaagaaaa
     3541 atggaaatac agcaagtttt attaaaagcg taacagatag attagaagat gagcattatg
     3601 atgtagaatt tgcatttcct caggattatg atatcaattt ttgtgatggc agtaatgaca
     3661 tttttataga tactgactac agtttaaatg atgaactaga gatattacaa aataagatac
     3721 tagaatcaga catattcatt ataggctctc ctgtatatgt tcattctatg tcagcagatt
     3781 taaaattatt tatagagcgc tctgcttggt gggtgcatac actaagactg caagggaaac
     3841 ctgttatagt aatgagtacc tgtggctcga atggattaaa gactgtaatt gaaccgttaa
     3901 gtgaagtaat aacatttatg ggtgggaatg tcattgctac ggcaaatgct acacaaatcc
     3961 cagatagact taatgataag gtatggatta aagaaatttc tgaagaaata attacacgaa
     4021 taaatacgta ttctgaattg ccaccgatgt caaataaatt tcttgagaaa gtttttaatg
     4081 gatcaaaact aaatatatta gaacaattaa aactagaaga caaagtgaat actaaatttg
     4141 gtgaactagt gtattggcaa aaaacaggaa tgattaattt tgataatttt agtagttatt
     4201 tagaatatat tcagagaaag tagataatat ttatgaaaat acggtttcaa caacaaagtg
     4261 aacactctga gtgtggattg gcatgtgcaa ctatgttgat tgattactgg agtgaaaaag
     4321 taaatttaac tgaaatgaga gaaagatatg gcgttcctaa tggaggatat aatctggctc
     4381 aaattcagtt gattctaaga gatgctgata tagatgtaaa agcagttaaa agtgaagcta
     4441 aatccattaa tgctgttccg gtacctttta ttgcattctg gaaaaataaa cattttatta
     4501 ttgttgaaaa ggtaacacat aaacatattg tagttgtcga tccagctaga ggaaaaagaa
     4561 aaatttctca tttggagttt gaagatggtt attctaacat ttgtatgtac attacaacaa
     4621 aaagaacaag aaaatatcaa cagcctaaat taaatccagt tttaaaagaa ttagttaaaa
     4681 ataataaaaa aaattttata aaaacttttt ttatattttt ggtaattcaa tgtataagta
     4741 tttacattcc ttacgcaata cgttggatta ttgatggtga agaaaaagat atgagaaaga
     4801 ttatttttta tgcctcattt ttaattagtt catattttat agcaaatgtt tttcgaacta
     4861 aactgatagt attattacaa acatcaacag ataaggactt attaagtaga acaattattc
     4921 aattattaga tttgccttat tcttattttg taaatcggag caagggagaa cttatttata
     4981 gaattaactc caatgcatat ataagacaaa tattgattga tcaagttatt aacaccgtga
     5041 ttgatttatt tttctttgca ttctatttaa ttgtaatgtg gacatttaat aaaacgttaa
     5101 ccataattac aatttttatt gcaatagtta ctatattttt ttcgtattta aatacaaaaa
     5161 tcatgaaaaa aattgcacag aatgagatgg tagtattaac taactcacaa gaattaatca
     5221 atgaaatggt caataatatt ttcaccatta aatcaactaa ttctcaaaaa aatatgtatg
     5281 agaaatggga aaaaaattat gatgaacaaa taatttatga aaaagaaaaa gccaaataca
     5341 attcttttct tgcgaatatt ccgcaaacaa ttcaaacatt ttattcactt ataatatatg
     5401 cagtgggtta ttttcttatt attaacagta tgacaacaat aggtaatatt gttgctttta
     5461 gtacaatagg gatttctttt ttaacaccaa taacttctat tctaaattct tatacacaat
     5521 tccaaacagt aaaagtttat ttagataggc tatttgatat actagaaact aaaagtgagt
     5581 cttctctttt aggaaatgaa gaactgaaag attattctgg agatgtaact attaaagatg
     5641 ttatgtacaa atatagtagt ttttctgagg aagcaatcaa aaatatttca ctaactatta
     5701 aacctcgtca aaaagttgct attgtaggtg ccagtggatc aggaaaatca accttattaa
     5761 aagtagtagc tggattatac caaagcagta aaggaaatat atattatggt aatcaaaata
     5821 ttaaagattt agacattcat aaactccgtg attccatagg tgttgttttg caagaaaatg
     5881 tactatttaa tgggacgttt agggaaaata ttacgatggg gagaaatcat acggacaaag
     5941 aaattacaga aataattaaa gcagtaggct tatctgaact agtttctagt tttccgttag
     6001 cattagagac ccaaatatct gagttggggc aaaatatgtc aggtggacaa cgtcaaaaag
     6061 tttcaatagc cagaacaatt ataacaaaac ctaaaattat ctttttggat gaacctacta
     6121 gtgcattaga taatatatct gaaaatgttg ttatgaactg cttatttaat attgacacca
     6181 cgcttattgt ggtggctcat cgtttgtcga ctattcaaaa tttcgataaa attgttgtta
     6241 tggataaagg agaagtggtt ggagttggga atcatgctga gttactaaat acaaataaat
     6301 actatcagaa tttatatcaa taaatactat caaaatttat atcaacaagg ctgttcttat
     6361 actatctatc aaaggaggaa actaaatatg agaattttag ttaaaaatat tttaagtatt
     6421 atatctagtg ttttgatttt tgttgttctt tcaggaaata gggttgttgc tttcgctcag
     6481 gaaccgtcaa tcgatttact agtgtctgaa ggagaaagta ttgagcaact aaaagaagag
     6541 ttgactgaag tagataataa actcgaattt atagagattc cagaaattaa tttattaaga
     6601 attgaaaatc caaatgagaa agtgcaagaa gttatagaga atagtgatga aattgatttt
     6661 tcaggaaaaa tttcagataa gctaatatta gaaaataata caataacaga aaaaaatgtt
     6721 gattttccaa aaatatttca gtattctgca gaggaacaaa atattgaaac tgaattgtta
     6781 aataggttaa cttggtacat taacacacta actcaagata aaaaagcttt tgagtattca
     6841 aaaggaaaaa acatcaaaat tggtctaatt gatagtggtg tggatacttc ccatccatta
     6901 atttctccct ctttaaacct agaaaaagca aagtcatttg taaaaaatga tcaatcaata
     6961 gaggattcca acggtcatgg aacgatggtt gctggagtca tttctcaagt gtcacctgaa
     7021 gctaaaatta ctccttacag agtcatgtca gcaattgatg gagaatctat atggacttta
     7081 caagctataa ttagagcagt caatgataag caagatataa taaatatgag cttaggaacg
     7141 tataaatatg aaactaaaaa ggatgaaaga attacaatag aagcatttaa aagagctgct
     7201 tgctatgctt ttataaaaaa agtgttaatt atttcttcgt cggggaacca acaacttgat
     7261 tctgatgtaa attatagaga aaataaaatt atgcatttac ctggagacat taagggtgta
     7321 ataactgtta gtgcaataaa taaaaataat cagttgacta attatagtaa tactggtact
     7381 aatgtacaat atacagcacc tggtggagag attattatag atgaaaatgg ttatttggat
     7441 gcaagagagt taatttatac catgtatccg ttgaccttac ctaatcctat gaaggacgct
     7501 ggtatacctg atggatatac tttaacctat ggaactagtt tttcatcggc aggtgttact
     7561 gcagtctttg ctaattatta ttcttattat ttacaaataa tgcaaaagaa accaaattca
     7621 caaaacgctc ataaattcat tgcttataat agtactgatt ttggcgttgt tggcaaagat
     7681 agtcaatatg ggtatggttt gcccaatctg attagagcct atgaattaat ttcaagtaat
     7741 aaaaatcatt aaagttaatt tgttattaaa agtaaattca tcaatattga acgaatttgc
     7801 ttgtaataaa atatatgtgt ttaggaggat atatcgtatt taagtgtaaa tagtcaaaaa
     7861 aatttaggag gagattatta tgtcagagtc aaatatgaaa aaagtagttg gagaaacgtt
     7921 tgaagatttg tcaattgcag aaatgacaaa agttcaagga tcaggtgatg tcatgccgga
     7981 atctactcca atttgtgctg ggtttgcaac actaatgtca agtattggtt tagttaaaac
     8041 aataaaagga aaatgttaaa tcgttctcag ttctaatata aaatatcctt ctgaaattta
     8101 ttacattatt ttagaaggat atttttttgt gagaatatat attaacttta aaaatacaca
     8161 taaaaggtgg gaaagtacaa atatttctaa tgtaaaaaac ttttatcatt aaaattagca
     8221 gattataaat atatttgata gactgtataa gaggtaatcg cctatcatgt tgttgaaggg
     8281 aggaatttaa ttgaaattgg ataaacaaat tcaatcaaaa agaaagaatt tgaatttgtc
     8341 acaggaagca cttgcagata aaatattcgt ttctagacag actatttcga actgggagaa
     8401 tgggcgaagt tatccagata tacaaaactt gttattactt agtgcgattt ttgatgttac
     8461 tttagacgaa ttagtgaaag gggatataga agtaatgaag aaaaaagttt ctagttcaaa
     8521 aatggataaa tatactatta taatgatcag tttcattcta ttgtcaatgt tgtcaattgg
     8581 tccatcctta tatttaccaa gctattgggc ctttttacca ccaatagtac tttggctagt
     8641 tggtatgtat ggagcaataa gagttgaaaa aatgaaaaaa acggaaaata ttagaactta
     8701 taaagaaata atcaatttta tggaaaataa agatagtata aaagataaca atcaagacag
     8761 taaatctaaa gatagattgt tattgattat aactgtgata ttatttagtc ttattggtgg
     8821 aggaattgca ttaggatcaa tatggttaat caatatttta taa
//
