LOCUS       BGC0000506             14098 bp    DNA     linear   BCT 25-JUL-2011
DEFINITION  Bacillus subtilis subsp. spizizenii DSM:15029 entianin gene cluster,
            partial sequence.
ACCESSION   BGC0000506
VERSION     BGC0000506
KEYWORDS    .
SOURCE      Bacillus spizizenii
  ORGANISM  Bacillus spizizenii
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:45:33
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..14098
                     /culture_collection="DSM:15029"
                     /db_xref="taxon:96241"
                     /mol_type="genomic DNA"
                     /note="type strain of Bacillus subtilis supsp. spizizenii"
                     /organism="Bacillus spizizenii"
                     /type_material="type strain of Bacillus subtilis subsp.
                     spizizenii"
     protocluster    1..14098
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[405:6631]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      406..6631
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..14098
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14098
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000506"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14098
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            406..3498
                     /gene="etnB"
     CDS             406..3498
                     /codon_start=1
                     /gene="etnB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     861.5; E-value: 1.2e-260)"
                     /gene_kind="biosynthetic"
                     /note="SpaB-like"
                     /product="EtnB"
                     /protein_id="AEK64491.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.7e-130,
                     bitscore: 425.6, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 7.8e-16,
                     bitscore: 48.3, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.1e-33, bitscore:
                     106.6, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSLYTPTDHYMIRVPLVHHDLKNGNSQDIDQLLHDLCNDSLFRE
                     QILVSSKTLYETINTFLQAPDKLKGKKKRNFQQAILKYATRRATRTTPFGLFSSVGIGS
                     FSDKNQLSFNQHSFYKKARVDFEWLYQLIRKLENEYTDRLSFTLNSACYIKGDRAYLLY
                     STDGKSEEVSVRATSVFYLINELCGESAAYQEIIRCLIDNYPNTPINKINQYVADLIDK
                     EFLISNLRPPMTVSDQFQYLIAQAESSHIPDELIQACKNIQYQIDAYNRITIGEGEHQY
                     LNLIETMNKFIKASSPLQVDAGLADSSIQLDNETSLAISELASMFTYMASPSAKKLDHL
                     EKYKNVFLERYGYEREVPLLEMLCSNTGIGAPATYTNPANEFFEETSFAEQFSPEMKQF
                     FMRKYFESVRKKAPIQLDDETFHRICNSEIADEEIPLSFELNFFVKLRNGRVMLYLGPN
                     VGSTRAGKTFGRFSHMSDSISDIIKTLHNKEKELTESNAKVCELSIVPNQTRSGNVTRN
                     VSYREKEMSLFTNSALHLHDSVKAEDVLIGINKDHIFYARHKTTGEILSFESNHMFNPL
                     LMTNAVRFLLEISRDGKRKWNDFPWFGIYSDFKYIPEIKYKEIILSCEQWLIYKSDLSM
                     HSNSSIEEVKSAFFEFHRTYELPQTFYIVNADNRLLIDIENDRTLDVFFWELKKTDHNQ
                     PLQLVAAERDVDALMDRNQNVYSGEIVVPLLRKQPEKPLYLPVLNAIEESGTDRIKMPF
                     EDWLFIKLYCKQTREEELIAFEIADFYNQISDQYPVRHFFMRYRDPKPHIRLRFNGKAE
                     VLYSLFPQLLNWLKSLREKGLVSESVITQYEREVERYGGLSLMEAAEQLFCEDSKAVET
                     IIRMHRMKDIEISKEIAGMVSVIQFLEQFELTFEEQLTFLERNSLQNEYRTEFKKDREM
                     YIEMCNSDRDWDNLKKTSDGRMLHDILKVRKMAAAHYAGLIKTAFDNKDAVYSRIGSVI
                     HLHCNRLFGTDRELENKILTLCRHSLYAQRYQKMNGSLAWK"
     misc_feature    <406..>12234
                     /note="entianin gene cluster"
     gene            3489..5333
                     /gene="etnT"
     CDS             3489..5333
                     /codon_start=1
                     /gene="etnT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 174.1; E-value:
                     6.2e-53)"
                     /gene_kind="transport"
                     /note="SpaT-like"
                     /product="EtnT"
                     /protein_id="AEK64492.1"
                     /transl_table=11
                     /translation="MEVKEQLKLKELLFIMKQMPRTIKLIFTLERSLFLKLILFSIITG
                     ILPIVSLYISQELINSLVTIRKDVSIVITIFLTYLGVSFCAELISQISEYYNGKFQLNI
                     GYKLNYKVMKKSSNLGLKDFENPEIYDKLERVTKEISYKPYQIIQAIITMTTSFVTLLS
                     SIAFLMSWNPKVSLLLLVIPVISLFYFLKIGQQEFFIHWKRAGKERKSWYISYILTHDF
                     SFKELKLYNLKDYLLNKYWDIKKSFIEQDTKILRKKTYLNLIYEIAVQLVGAVIIFVAI
                     MSAFAGKIMVGNVMSYIRSVSLVQNHSQSIMTSIYSIYNSNLYMNQLYEFLELKEEKSQ
                     GHKKQIVEPIHSVVFQNVSFMYPNQGEQTLRHINVSLHKGERIAIVGPNGSGKSTFIKL
                     LTGLYEVQQGDILINGISIKELDMDSYMNQIAALFQDFMKYEMTLKENIGFGQVDKLHQ
                     TNKMHEVLDIVKADFLKSHSSYQFDTQLGLWFEEGRQLSGGQWQKIALARAYFREASLY
                     ILDEPSSALDPIAEKETFDTFFSLSKEKIGIFISHRLVAAKLADRIIVMDKGEIVGIGT
                     HEELLKTCPLYKKMDESENYMNPLEEEGSKWKEALYQG"
     gene            5306..6631
                     /gene="etnC"
     CDS             5306..6631
                     /codon_start=1
                     /gene="etnC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     407.8; E-value: 7.8e-124)"
                     /gene_kind="biosynthetic"
                     /note="SpaC-like"
                     /product="EtnC"
                     /protein_id="AEK64493.1"
                     /sec_met_domain="LANC_like (E-value: 8e-89, bitscore:
                     287.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERGAVSGIEVEIVKEMARQISNYDKVLEIVNQKDNFRNIGEVPL
                     IPWKSTALSHGIPGICMLYGELHAHFPEEGWDDLGHQYLSILVNEIKEKGLHTPSMFSG
                     AAGIGLAAVCLSQHFTYYNGFIARINEYLAEVVPQLLTEFYQRQVYMSDYDVIEGVSGI
                     ASYLLLFQEDKAMKDLLIDILKYLVRLTEDITVDGEKVPGWHISSEHQFTDIEKKAYPN
                     GNFNMGLAHGIPGPICVLSSALMQGIKVKGQERAIEKMVDFLLEFSEKEQDSLFWKGII
                     SFEEYQYGSPPDAINFSRDAWCYGRPGVCLALVKAGKALHNTELLNIGVQNLRYTISDT
                     RGIFSPTICHGYNGIGQILLAVNQLTGQDYFKEELQEIKQKIMSYYDKEYIFGFHNYES
                     MEENEVVPLQYVGLLDGAVGVGLGVLNMELGSKTEWTKALLI"
     gene            6705..6875
                     /gene="etnS"
     CDS             6705..6875
                     /codon_start=1
                     /gene="etnS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_a"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="SpaS-like"
                     /product="EtnS"
                     /protein_id="AEK64494.1"
                     /sec_met_domain="TIGR03731 (E-value: 4.8e-24, bitscore:
                     73.8, seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_a (E-value: 3.6e-08, bitscore:
                     22.3, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKFDDFDLDVVKVSKQDSKITPQWKSESVCTPGCVTGLLQTCFL
                     QTITCNCKISK"
     CDS_motif       6705..6776
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MSKFDDFDLDVVKVSKQDSKITPQ"
                     /locus_tag="etnS_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       6777..6875
                     /prepeptide="core"
                     /locus_tag="etnS_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="27.80"
                     /molecular_weight="3349.0"
                     /monoisotopic_mass="3346.7"
                     /core_sequence="WKSESVCTPGCVTGLLQTCFLQTITCNCKISK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSKFDDFDLDVVKVSKQDSKITPQ"
                     /leader_location="[6704:6776](+)"
                     /alternative_weights="3367.0"
                     /alternative_weights="3385.0"
                     /alternative_weights="3403.0"
     gene            7364..7939
                     /gene="etnI"
     CDS             7364..7939
                     /codon_start=1
                     /gene="etnI"
                     /note="SpaI-like"
                     /product="EtnI"
                     /protein_id="AEK64495.1"
                     /transl_table=11
                     /translation="MDTHWQRNPFLLFNIYWGKFIRLRWCMLFLKRCVNLLGCFIVLFM
                     LSACQSLIKFKDKVVEETKKTMHFTDDNENDTSETMESLIDKGKLDQVVYDDKLYQLKD
                     KVDEDKKGKVIGAIGQTFFVDGDGKRWSEEELKEPYISNDPDEIREKKPLRYGKVYSTN
                     EDPDAKNEIIVEFNREYYRAVLIKNEKE"
     gene            7926..8669
                     /gene="etnF"
     CDS             7926..8669
                     /codon_start=1
                     /gene="etnF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 181.1; E-value:
                     4.6e-55)"
                     /gene_kind="transport"
                     /note="SpaF-like"
                     /product="EtnF"
                     /protein_id="AEK64496.1"
                     /transl_table=11
                     /translation="MKRNKGESDMSTVLLETKSLTKKFGKETAVSDVSLTVQKNSIYGL
                     LGPNGAGKSTTLKILTGILRKTSGEVFFDGHKWKRSDLQNIGSLIETPPLYDNLTAFEN
                     VKVHATLLGLSEDRIHSVLKTVDMEHAGKKRAGQFSMGMRQRLGIAIALLNHPKLLILD
                     EPTNGLDPIGIQELRDLIRSFPQEGITVILSSHILSEVEQIADHIGIIIGGHLGYQGEI
                     NKADDLEHLFMEVVKSSREKEGVDR"
     gene            8666..9421
                     /gene="etnE"
     CDS             8666..9421
                     /codon_start=1
                     /gene="etnE"
                     /note="SpaE-like"
                     /product="EtnE"
                     /protein_id="AEK64497.1"
                     /transl_table=11
                     /translation="MMNYIKAENLKFKRTFSRKMIIFVPLLNIGFSFLMNIQFFVSGTF
                     NWWSIIFMPVMIALFCSLSHQKEKKASNYNGTYSLPIDLGRLWYAKIIIIMFYSLLSQI
                     VFLVFMLLMGFVIADFAIITLSTIAASLLLWMTSLWQIPLCLFVAKKWGLTIAVMLNLI
                     GTFVLGIMPASRSFWWFIPWSWPIRMMSPTIGIHPNGLLLEKNDPLLSWLVVPPAILIS
                     ILLFLILSFLTSRSFAPSIDYRTKKQGAM"
     gene            9483..10193
                     /gene="etnG"
     CDS             9483..10193
                     /codon_start=1
                     /gene="etnG"
                     /note="SpaG-like"
                     /product="EtnG"
                     /protein_id="AEK64498.1"
                     /transl_table=11
                     /translation="MWLHFIIPIVCSCVLILYFYGREQSQFYLYKSFMEAVAVALPLLI
                     GVLCGMTASLEEQAGKFQVLLGSTSPKVIAYVSKMLMLLFMGLCSIGLALFIYIIGLKF
                     ILKVPDLSYGIFIMGGAWLVAGSVVLYCIYFFISCMFGMGASVLIGGAGLLMTALMNTG
                     LGDMMWKFNPWAWGIRLSGLNGVLHFKEVNQGDNPLLMHEMNSGELIMVFGIICTIIAS
                     LIWFSKWEGRKTFE"
     gene            10211..10873
                     /gene="etnR"
     CDS             10211..10873
                     /codon_start=1
                     /gene="etnR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 171.9; E-value: 2.5e-52)"
                     /gene_kind="regulatory"
                     /note="SpaR-like"
                     /product="EtnR"
                     /protein_id="AEK64499.1"
                     /transl_table=11
                     /translation="MAKILAVDDEKDILVLIQNILRRDQHQVDTLDRVKGQPPDLFQGY
                     DLILLDVMMPDIDGFELCKQIRPLVDCPILFLTAKTEEEAIVKGLITGGDDYITKPFGV
                     RELSARVNAHLRRERRDKHQSKRVISGFLFHFDSKEVFINNNKLNLTKNEYKICEFLAQ
                     HKGRTFSREQIYEEIYGLEGNALYSTITEFIRTIRKKCKEHNADPIKTVWGVGYKWE"
     gene            10846..12234
                     /gene="etnK"
     CDS             10846..12234
                     /codon_start=1
                     /gene="etnK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 135.6; E-value: 5e-41)"
                     /gene_kind="regulatory"
                     /note="SpaK-like"
                     /product="EtnK"
                     /protein_id="AEK64500.1"
                     /transl_table=11
                     /translation="MGRWIQMGIGFKGQKTLLRELIKYMVTLCISLVILALLYLFMNTI
                     AMNTGFSHPANYNEREAEKLAVKLESIDKVTADMIPDTMSYAILDKETKQKTAGNIKEK
                     DLQLVKKKIEKKPYVNYKQKGYLVIERNDEYCVLQYSLRADFTSPLLRKYLPNYELTSI
                     CILIILLIIVISIITTYFANRLRKHFETLNLITRYIKEQNLQFTPEFTHIKEFDDVIDS
                     LIEMRDALQSSLEAQWRLEKNKKEQIGALAHDIKIPITIIKGNAELLSLSIQNEEQAEY
                     TKYILGAGNQIEQYIYQLIHLSKTEDALTVHLEKASVDKLTETLLKDISAYKGNKNINI
                     SFKKENLMKEAKIDWQLLHRALLNILTNAVDYTPEGGTVSVHAECDSEIFYFFVKDTGN
                     GFSEMGLKKATELFYMDDKSRHSKGHYGMGLTFAKNAVNLHNGELTLGNTIAGGAEVRV
                     KIPL"
     gene            complement(12275..13975)
                     /gene="yvaQ"
     CDS             complement(12275..13975)
                     /codon_start=1
                     /gene="yvaQ"
                     /gene_functions="other (smcogs) SMCOG1061:methyl-accepting
                     chemotaxis protein (Score: 268.6; E-value: 1.9e-81)"
                     /gene_kind="other"
                     /note="putative methyl-accepting transducer"
                     /product="YvaQ"
                     /protein_id="AEK64501.1"
                     /transl_table=11
                     /translation="MRLTISRKFSLVFLTLILINLLVGGIGAFNMQHIIQKTDEINTKW
                     IDGIKEITSINYLTEHLSSKEKDFLIFTDKSKMDTLDQEMNQILEDINQKLDSYEKTIS
                     NDKEQKLFEELQNEVNTYADIHAQIIESGRTNDMDKARGLLVQTEASFENMKKSVTQLV
                     DFNKEGSNTAVKETKDVYHKGLIYTASLVAASIIISIFIWLYITRNIVKPIIRMKESAN
                     HIAEGDLSSDIEPLNSKDELGDLNEALQKMVGNLRDIVGYSKEISSRVLSSSQVLATAT
                     NETRSGSKHITETMNEMAEGSEQQAQDAVTIAESMNEFTESIDKAYNHGITISDTSQNV
                     LELAVSGNENMDTSLQQMKTIHHIVQEAVHKVRSLEQHSQDINKLVQVINGIAEQTNLL
                     SLNAAIEAARAGESGKGFAVVAEEVRKLADGVSDSVQDITRIVNGTQQEIYTVIEYLES
                     SFTEVEKGTENLTDTGQAMQHIKQSVTHVADSIKEVTDGLKQLTNQSITINQSIENIAS
                     VSEESAAGIEETFSITEQSAHSMDQVLQNAEELEQLAKELNEKMNQFTI"
ORIGIN
        1 ggcgccgggg ggctcgggga tatgatcgtc cgtggctcaa atgcgacaaa cggaactgcg
       61 attattctcg ctggcgcgat cccgactgcg gtgatggcca taggagcaga tctgataatg
      121 gcctggattg aaaggttgtt gaatccagtg aaacaaaaaa atagaaaaaa gttaataagt
      181 gtatagagtt aagcaaaatg gattcttttc tgagagggaa aagagtcctt ttttatggta
      241 tttattgggt ggatcttgat atttttttga tttttagaat gtatagtaca aatagaatat
      301 tgtaacaatt ttggtttaaa tagaataaaa tggaggactc tgcagaggga cgtagaattg
      361 ttgttattat atgaagtgga taaatatggg gggcaataga ctaatatgaa atccttatat
      421 acacctacag atcattatat gattcgggtt cctttagtac atcatgacct aaaaaatggg
      481 aactcgcagg atatcgatca gttattacat gacctttgca acgattcatt atttcgggaa
      541 caaatactgg tatctagcaa gacactatat gaaacaataa atactttcct gcaagcgccg
      601 gataaattaa aagggaaaaa aaagcggaac tttcagcagg caattttgaa gtatgcaaca
      661 agaagagcaa caagaacaac accttttggc cttttttctt cagtcggtat agggtctttt
      721 tccgataaaa accaattatc ttttaatcaa cattcatttt acaaaaaggc tcgtgttgat
      781 tttgagtggc tctatcaatt aattagaaaa ctggaaaacg aatacactga ccgactttct
      841 tttacattaa attccgcttg ttatattaaa ggtgaccggg cttacttgtt gtacagcaca
      901 gatggaaaat ctgaagaagt tagtgttcgt gcgacatcgg ttttctattt gataaatgaa
      961 ctttgtggtg aatctgctgc atatcaagag attatccgtt gtttgataga taactatcca
     1021 aatactccaa tcaataaaat taatcagtac gtagcagacc tgattgacaa agagttcctt
     1081 atatctaact tacggccgcc gatgactgtt tcagatcaat ttcagtattt aattgctcaa
     1141 gcagaaagca gccatattcc agatgagctc attcaggctt gtaagaacat tcaatatcag
     1201 atagatgcat ataatcggat cactattgga gagggagagc atcagtattt aaatctgatt
     1261 gaaacaatga ataaattcat aaaggcatca tctcctctgc aagtagacgc tggattagca
     1321 gactcctcca ttcaactaga taatgaaaca tctcttgcca taagtgaatt ggcaagcatg
     1381 ttcacttata tggcttctcc ctctgcgaag aaattagacc acttagaaaa gtacaaaaat
     1441 gtgtttttgg aacgctatgg atatgaaaga gaagttccgc ttttagaaat gctttgttcc
     1501 aacactggta tcggtgcgcc tgctacctac acgaatcctg ctaatgaatt cttcgaagaa
     1561 acatcatttg cggagcaatt ttcaccggaa atgaaacaat ttttcatgag aaagtacttt
     1621 gaatcagtta gaaagaaagc tccaattcaa ttagacgacg aaacattcca tagaatttgc
     1681 aactctgaaa ttgctgatga agaaattcca ttatcatttg aactcaattt ctttgttaaa
     1741 ttacgtaatg gcagagttat gctttattta ggccccaacg tcggatctac ccgcgcaggg
     1801 aaaacatttg gaagattttc tcatatgtct gattcaatca gtgacatcat aaagacctta
     1861 cataacaagg agaaagagtt aacagagagt aatgcgaaag tttgtgaact gagtattgtg
     1921 cctaatcaaa ctaggtctgg aaatgtaaca agaaatgtta gctaccgaga aaaagagatg
     1981 tctcttttta cgaacagtgc tctgcatctc catgattctg tcaaagctga agatgttcta
     2041 attggaatca ataaagacca tatcttttat gctagacata aaacaactgg cgaaattctg
     2101 tcttttgaat caaatcatat gtttaatcct ttattaatga ccaacgctgt acgattttta
     2161 ttagagattt caagagacgg taaaagaaaa tggaatgatt tcccgtggtt tggcatctat
     2221 agtgatttca agtatattcc tgaaatcaaa tataaagaaa taatcttatc gtgtgaacaa
     2281 tggctaatat acaaaagtga tttaagcatg cactcgaatt catcaataga agaggtgaaa
     2341 tctgcttttt ttgaatttca tcgtacttat gaactgccgc aaacatttta tatcgttaac
     2401 gcagataatc ggttattaat tgatatagag aatgatcgta ctttggacgt ttttttctgg
     2461 gaactgaaaa aaacggacca taaccagcca ttgcaacttg tggctgctga gcgtgatgta
     2521 gatgcgttaa tggatagaaa ccaaaatgtc tattcgggag aaatcgtcgt tccgctgctc
     2581 agaaaacaac cagaaaaacc attgtattta ccggttctta acgcaataga ggaaagtgga
     2641 accgacagaa taaaaatgcc atttgaagat tggctgttta ttaagcttta ttgtaaacaa
     2701 acaagagaag aagagctaat tgcttttgaa atagcagatt tttataacca gatttctgat
     2761 caatatccag tcagacattt ctttatgagg tatcgggatc caaagcctca tataagactt
     2821 agatttaatg ggaaagccga agtgctgtac agcttgtttc cccaattatt gaattggctg
     2881 aaaagcttaa gagaaaaagg gctggtttca gagtctgtta tcacccaata cgagcgggag
     2941 gtagagcgat atggcgggct aagcctaatg gaagctgcag aacagctttt ctgtgaagac
     3001 agcaaagctg ttgaaacgat aatcagaatg caccggatga aggatattga gataagcaag
     3061 gaaattgcag gcatggtttc tgttatacag tttttagaac agttcgagct tacgtttgaa
     3121 gagcagttaa cttttttaga gagaaattct ctgcagaacg agtatcgtac tgaatttaaa
     3181 aaggatagag aaatgtatat tgaaatgtgc aattctgata gagattggga taatctcaag
     3241 aaaacaagtg atggcagaat gttgcatgac attttgaaag taagaaaaat ggctgcagct
     3301 cattatgcag gtttaatcaa aacggcattt gataacaaag atgcggttta ttcacgtata
     3361 ggaagtgtca tccacctgca ttgcaatcgt ttattcggaa ccgacagaga actagaaaat
     3421 aaaattctta cactttgcag acattcttta tatgcacaac gatatcaaaa gatgaatggt
     3481 agcttggcat ggaagtaaag gaacaactga agctaaaaga gctgctgttt atcatgaaac
     3541 aaatgcctag gactatcaaa ttgattttca ccttagaaag gtcactgttt ttaaagttga
     3601 ttttattcag tattattacc ggtatcctgc caattgtttc actatatatt tcacaagaac
     3661 tgattaattc ccttgtgact atccgaaaag atgtttcaat cgttatcacc attttcttga
     3721 catatcttgg ggtatcattt tgtgcggagc tcatctctca gatttctgaa tattataatg
     3781 gtaaattcca attaaatatt ggttataaac taaactataa agtcatgaaa aagagcagta
     3841 atttaggcct gaaggatttt gaaaatccag aaatatacga caaattagaa agggtaacca
     3901 aagaaatcag ctataaaccc tatcagataa ttcaggccat cattacaatg accacatcat
     3961 ttgtgaccct gctctcttca attgcgtttt tgatgtcatg gaaccctaaa gtctcactgt
     4021 tattgttagt tattccagtg atttctctat tctatttctt gaaaattgga cagcaagaat
     4081 tttttataca ttggaaacga gcaggaaagg aaaggaaatc atggtatatc agctatatac
     4141 tcacacatga cttttctttt aaagaattaa aattgtataa ccttaaggat tatttattaa
     4201 acaagtactg ggatatcaaa aaatcattta tagaacaaga tacaaaaata ttgagaaaga
     4261 aaacttattt gaacctaata tatgagattg cagtgcaatt agttggagct gtcattatat
     4321 ttgttgcaat catgtctgct tttgcgggaa aaattatggt cggtaatgtg atgagttata
     4381 tcagatctgt ttctctggtg caaaaccact cacaatctat catgacaagc atttattcca
     4441 tatataacag caatctctat atgaatcaat tgtatgaatt tctggaatta aaagaagaga
     4501 aaagtcaagg tcacaagaaa caaattgttg agcctattca ttctgttgtc tttcaaaatg
     4561 tcagctttat gtatcccaat caaggagaac agacactgag acatattaac gtttcattgc
     4621 ataaaggaga acgaatagct attgttggac caaacggatc aggaaaaagt acattcatta
     4681 agctccttac cggcttatat gaagttcagc agggtgacat tttgattaat ggaataagta
     4741 ttaaagagct ggatatggac agttatatga atcaaattgc agcactattt caagacttta
     4801 tgaaatatga aatgacatta aaagaaaata taggtttcgg gcaagttgat aaactgcatc
     4861 aaacaaacaa aatgcatgag gttctcgata ttgtgaaggc cgatttctta aaaagccatt
     4921 cttcctatca attcgataca cagctcggac tatggtttga agaaggaagg cagttatccg
     4981 gtggacaatg gcaaaagatt gctttggcaa gggcttattt tagggaagct tctttgtata
     5041 ttttggatga gccaagttct gcgctggatc caattgcaga aaaagaaacc ttcgatactt
     5101 ttttcagttt gtcaaaagaa aaaataggaa tatttatttc ccatcgattg gttgctgcta
     5161 aacttgcaga tcggatcatt gtaatggata aaggagaaat tgttggaata ggcacacatg
     5221 aagagctatt aaaaacatgt ccgctgtata aaaagatgga tgaatccgag aattatatga
     5281 atccactaga ggaggaaggc agtaaatgga aagaggcgct gtatcaggga tagaagttga
     5341 aattgtgaag gaaatggctc gtcaaatttc taattacgat aaagtgctgg aaatagtgaa
     5401 tcaaaaagat aactttcgca atattggcga agttccgctt ataccttgga agtcaactgc
     5461 tttgagtcac ggtataccag gcatttgtat gctgtatggc gaattgcacg ctcattttcc
     5521 tgaggaagga tgggacgacc taggacatca atatttatcg attttagtaa atgaaatcaa
     5581 agaaaaaggg ctgcatactc cttcaatgtt ttctggagca gctggcattg gactggcagc
     5641 agtctgttta tcgcaacatt tcacctatta caatggtttc attgctcgta ttaatgaata
     5701 tttggctgaa gtcgtacctc aattgttaac tgaattttac cagcggcaag tgtatatgag
     5761 tgactacgat gttatagaag gagtcagcgg aattgcaagc tatctgctgc tatttcaaga
     5821 agataaggct atgaaggatt tgttaattga tattttaaaa tatctggtta ggttaactga
     5881 agacattacc gtagatggag aaaaagttcc tggatggcat atttcatctg aacatcagtt
     5941 tacagatatc gaaaaaaaag cttatccaaa cggaaacttt aatatgggat tagcacatgg
     6001 aatacctggc cctatttgtg ttctgtcttc agcactcatg cagggaatta aagtaaaggg
     6061 ccaagaacga gcgattgaaa aaatggtgga tttcctactg gaattttctg aaaaagagca
     6121 agacagcttg ttttggaaag gaatcataag ctttgaggag tatcaatacg gatctccgcc
     6181 ggacgcaata aattttagca gagatgcttg gtgttatgga agaccaggtg tatgtctagc
     6241 gcttgttaaa gccggaaaag cacttcataa tactgaactt ttaaatatcg gagtacagaa
     6301 tttaagatac acaatatctg atacacgggg aattttttca cctaccatat gccatggata
     6361 caacggtatc ggtcagattt tgcttgccgt aaatcaactc accggacagg actattttaa
     6421 ggaagagctt caagagatta aacaaaagat tatgagctac tatgacaagg agtatatctt
     6481 tggattccat aactatgaat caatggaaga gaacgaagta gtacctttgc agtacgttgg
     6541 tctgttggat ggagctgtag gtgtagggtt aggagtatta aacatggaat taggctcaaa
     6601 aacagagtgg acaaaagcat tattaattta ataaaaaaag gaaaaaatga taaaatcttg
     6661 atatttgtct gttactatat aggtattgaa aggaggtgac caatatgtca aagttcgatg
     6721 atttcgattt ggatgttgtg aaagtctcta agcaagactc aaaaatcact cctcaatgga
     6781 aaagtgagtc agtttgtaca ccagggtgtg taactggtct attgcaaact tgcttccttc
     6841 aaacaatcac ttgtaactgc aaaatctcta aataagtaaa accattaatc actttgcccc
     6901 gactccttgc acttctgagc gttatacata cttattttca tagagttggg gcatgaaaac
     6961 tatgtaaaaa gcaacgggtg tgaaagagtt tatatttcac acccgttttt tatattcagc
     7021 tttatgaatc gataaattgc agtggggata aacggataat tctataatga agtttaaaca
     7081 accttcgtca agtacagaca agggatgaat gactcaaggg ttcttaatag ggtttatggt
     7141 taagaatatt tctaagaaaa tcatatttta atttggagat caataaatga gattgatcca
     7201 tctagagtaa ggagtgtgac ctgatcacac tttttttatt tggcatgtga tcgtggtgct
     7261 agccgcccaa atgcttaaag tttccagttg gaaatgtttt tttgattaaa ttttgataaa
     7321 agtattctag aatgatctgc atccggtaaa aaagaatctt cacatggata cacattggca
     7381 aaggaatcca tttttgcttt ttaacatcta ttggggaaaa ttcataagat tgaggtggtg
     7441 tatgttgttt ttgaaaagat gtgtaaacct tttaggctgt tttattgttt tatttatgtt
     7501 atctgcttgt caatcattaa taaagtttaa agataaagtt gttgaagaaa caaaaaaaac
     7561 aatgcatttt actgatgata atgaaaacga tacatctgaa acaatggaga gcctgattga
     7621 taagggaaaa ctcgatcaag tggtctatga cgataaactt tatcagctga aagataaagt
     7681 tgatgaagac aaaaaaggaa aagtaatagg ggcaataggc caaactttct ttgtagatgg
     7741 tgatggaaag agatggtcgg aagaagaact gaaagaaccg tatatctcta atgacccgga
     7801 tgaaatcaga gagaaaaaac cattacgcta tgggaaggtt tacagtacaa acgaggatcc
     7861 tgatgccaaa aatgaaatca ttgttgagtt taatcgtgag tattatcgag cggttttaat
     7921 aaagaatgaa aaggaataag ggagagagtg acatgagtac agtcctatta gaaaccaaaa
     7981 gccttacaaa aaagtttggc aaagaaacag ctgtttcaga tgtttcatta actgttcaaa
     8041 aaaacagcat ttatggtctg ctgggaccaa atggagctgg aaaatcgaca acacttaaaa
     8101 ttttaacagg aattttacgg aagacttctg gtgaggtttt cttcgacgga cataaatgga
     8161 aacgatctga tttacaaaat atcggatcgt taatagaaac tccaccactt tacgataatt
     8221 taactgcatt tgaaaatgtg aaagtccatg ccacgctgct cggtctttct gaagatcgca
     8281 ttcacagtgt gctaaagact gtggatatgg agcatgctgg aaagaaaaga gcagggcagt
     8341 tttcgatggg aatgaggcag cggcttggaa ttgcaatcgc cttattaaat catcctaaat
     8401 tattaattct tgatgagcct acaaacgggc tagatccaat aggtatacag gagcttaggg
     8461 acctaattcg ctcatttcct caggaaggaa taacggtgat tttatctagc cacattcttt
     8521 cagaagtcga acaaatagca gatcatattg ggattatcat cggcggtcac cttgggtatc
     8581 agggtgaaat aaacaaggct gatgatttag agcacttgtt tatggaagtt gtaaagtcaa
     8641 gccgtgaaaa agaaggtgta gatagatgat gaattatata aaagccgaaa acctgaagtt
     8701 taagaggacg ttttccagaa aaatgattat ctttgtacct cttcttaata taggtttttc
     8761 ttttttaatg aacattcaat tttttgtatc cggtactttt aactggtggt ctattatatt
     8821 tatgccggtg atgattgctt tattttgttc tttatctcat caaaaagaaa aaaaggcttc
     8881 taattataat ggtacgtatt cattacctat agatttaggg agattatggt atgcaaagat
     8941 tataattatc atgttttatt cgctattatc tcagattgta tttttagttt ttatgttact
     9001 catgggattc gttatagctg attttgccat tattacgctt tctacaattg ccgcgagttt
     9061 gttactctgg atgacaagtc tttggcaaat tccactctgt ttatttgtgg ccaaaaagtg
     9121 ggggcttaca atagcggtaa tgttaaatct tataggaacc ttcgttctgg gcattatgcc
     9181 ggcatctcgt tccttttggt ggtttattcc ttggagctgg ccaattcgaa tgatgagtcc
     9241 aactattggt attcatccta acggtctgtt gcttgaaaaa aatgatcctt tgttgagttg
     9301 gctggtcgtc cctcctgcta tattgatttc tattctatta tttttgatac tgtcattttt
     9361 aaccagccgt tcatttgccc cttcaatcga ttacagaaca aaaaagcagg gggcgatgta
     9421 aggtgagatt gcttttgagg tgtttacaag ctgatttcca gaaaagcaag cacacctcct
     9481 ttatgtggct gcattttatt attcctatag tttgttcatg tgtattgatc ttgtattttt
     9541 atgggagaga acaaagccaa ttttacttgt ataaatcttt tatggaagct gttgcagtag
     9601 cattgccgct gctcatcggg gtgctatgcg gaatgactgc atccttagaa gaacaggcag
     9661 ggaaatttca agtgttacta gggagcacat ctccgaaagt cattgcttat gtgagtaaaa
     9721 tgttaatgct gcttttcatg gggttgtgct ctattggttt agcgctattc atctatataa
     9781 taggattaaa gtttatttta aaagtgccgg atttgtctta cggtattttt ataatgggtg
     9841 gtgcctggct agttgcgggc agtgtagtcc tgtattgtat ttactttttt atcagctgta
     9901 tgtttggaat gggggcttct gtcctgatag gaggagccgg cctgctcatg acagctctaa
     9961 tgaacactgg attaggggat atgatgtgga aatttaaccc gtgggcttgg gggattcgat
    10021 tgtcaggttt aaatggagta cttcacttta aagaagtaaa tcaaggtgat aatcctttgt
    10081 taatgcatga aatgaattca ggggaattga ttatggtttt tggtataata tgtacaatca
    10141 ttgccagtct tatatggttt agtaaatggg aaggcagaaa aacgtttgaa tagtatgagg
    10201 gggccgggaa atggcaaaaa tccttgctgt cgatgatgaa aaagatattc ttgtactgat
    10261 acaaaatatt ttgcgccgtg atcagcatca agttgacact ttagatcgtg ttaaaggcca
    10321 gccgcctgat ttatttcaag ggtatgattt aattttgctt gatgttatga tgccggatat
    10381 tgacggtttt gagttatgta aacagatacg gcctttggtt gattgtccaa ttttattcct
    10441 cacagctaaa acggaagagg aagcgatagt aaaaggcctc attaccggcg gagatgatta
    10501 tattactaag ccgttcggcg ttagagagct tagtgcccga gttaatgctc acttaagaag
    10561 agaacggaga gataaacatc aatcgaagag agttatctcc ggttttttat ttcattttga
    10621 tagtaaagaa gtatttatta ataacaacaa actcaaccta acaaaaaatg aatataaaat
    10681 ctgtgaattt ttagctcaac ataaagggag aactttttca agagaacaaa tttatgagga
    10741 gatatatgga ttagagggga atgccttgta ttcaacaatt actgaattta ttcgaacgat
    10801 tcgaaaaaaa tgtaaagagc ataatgcaga ccccattaaa acggtatggg gcgttggata
    10861 caaatgggaa taggttttaa ggggcagaaa acacttctta gagaactgat aaagtatatg
    10921 gtgactctat gtattagttt ggtcatactt gctctgctat atttatttat gaatacgatc
    10981 gcaatgaata caggtttctc acacccagcg aattataatg aacgagaagc agaaaaactg
    11041 gcggtgaagc tggaaagcat cgataaagta actgctgata tgattcctga tacaatgtca
    11101 tatgccatcc ttgataaaga aacaaaacaa aaaacagctg gaaatattaa agaaaaagat
    11161 ttgcagcttg taaaaaagaa gattgaaaaa aaaccatatg tcaattataa gcaaaaagga
    11221 tatttggtta tagaaagaaa cgatgaatat tgtgttttgc aatattcatt gcgtgcagat
    11281 ttcacttcgc ctttactaag aaaatacctt cctaattatg aattaacgag tatttgtata
    11341 ttgattatct tgctcattat agttatctct attataacaa cttactttgc gaatagatta
    11401 agaaaacatt ttgagacatt gaacttaatc actcgatata taaaagagca aaatttgcaa
    11461 ttcactcccg aatttacaca tattaaggaa ttcgatgatg tgattgactc cttgattgaa
    11521 atgagagatg cccttcagtc ctcacttgag gcacagtggc gtttagaaaa aaacaagaaa
    11581 gaacaaatag gtgctttggc acatgatatc aagattccga ttaccattat taaggggaat
    11641 gctgaattac tcagcttgtc aatacaaaac gaggaacaag ctgagtatac aaagtatatt
    11701 cttggagcag gaaatcagat tgaacaatat atttatcagc tgattcatct ttcgaaaacg
    11761 gaggatgcct taactgttca tttggagaaa gcatctgtag ataagttgac agaaacccta
    11821 ctgaaagata tatccgccta taaaggaaat aaaaatataa acatttcgtt caaaaaagaa
    11881 aatctaatga aagaagcgaa aatagactgg caattgttac atagggcatt attgaatatc
    11941 ttaacaaacg cagttgatta tacaccggaa ggcggcactg tctcagtaca tgcagaatgc
    12001 gattctgaga tattttattt ttttgtaaaa gacacgggaa atggattttc ggaaatggga
    12061 ttaaaaaaag ccacagaact tttttatatg gatgacaaga gccgtcattc taaaggtcat
    12121 tatggaatgg gattgacttt tgcaaaaaat gcagtgaatc tacataatgg agaacttact
    12181 cttgggaaca ctatagcggg tggagcagag gttcgagtaa aaataccctt atgaaacgaa
    12241 taaagcccac gtcctatctg gacgtgggtt atttttaaat ggtaaactga ttcatcttct
    12301 cattaagctc tttcgccagc tgctcaagct cttcggcatt ctggagcact tgatccatgg
    12361 aatgggcgga ttgctcggta attgaaaatg tttcttcaat gccggcggcc gattcttcag
    12421 atacagaagc gatattttca atcgattgat tgatggtaat cgattggttt gttaactgtt
    12481 tcaggccatc cgttacttcc ttaatgctgt ctgcaacatg agtgacggac tgtttgatat
    12541 gctgcatagc ttgtccggtg tctgtcaggt tttccgttcc tttttcgact tctgtaaagc
    12601 tgctttccaa gtattcaata acggtgtaga tttcctgctg tgtgccgttg acaatccggg
    12661 taatatcctg cacggaatca gaaacgccgt cagccagctt tctgacctcc tctgccacaa
    12721 cagcgaagcc tttgccgctt tctcctgcgc gggcagcttc aatcgcagcg ttaagtgaca
    12781 gcaaattcgt ttgctcggca atgccgttga tgacctgaac gagcttgttg atatcctgag
    12841 aatgctgttc cagtgatctc actttatgca cggcttcctg aacgatatga tggatggttt
    12901 tcatttgctg taatgacgta tccatatttt cgtttccgct cactgcgagt tcgagaacgt
    12961 tttgagacgt atcgctgatt gtgatgccgt gattataggc tttatcaatg ctttctgtaa
    13021 actcattcat tgattcggca attgtcaccg cgtcttgggc ctgctgttca gagccctcag
    13081 ccatttcgtt catcgtctcg gtaatatgtt tgctgccgga tcttgtttca ttcgttgcag
    13141 ttgccagcac ttgtgaagaa gaaagcaccc ggcttgatat ttctttggag tatccgacaa
    13201 tatctctcag attgcctacc attttttgca gcgcttcgtt cagatcaccc agctcgtctt
    13261 ttgaattgag cggctctatg tcactcgaca gatctccctc ggcaatatga ttggcagatt
    13321 ccttcatacg aatgataggc tttacaatgt ttcgcgtgat ataaagccag atgaaaatgc
    13381 tgataatgat cgaagctgcc acaagcgaag ccgtgtaaat gagtccttta tggtatacat
    13441 ccttcgtttc ttttaccgct gtgttgctgc cttctttatt aaagtcaacg agctgagtga
    13501 ctgatttttt catattctca aagctggcct cagtctgtac caataaacct cttgctttgt
    13561 ccatgtcatt cgtgcgtccg ctttctataa tttgcgcatg aatatcagca taggtattca
    13621 cttcattttg aagctcctcg aacagtttct gttctttatc attggaaatc gttttttcgt
    13681 agctatcaag cttttgattg atgtcttcaa ggatctgatt catttcttga tcaagcgtat
    13741 ccattttgct tttgtctgtg aaaatcagaa aatccttttc tttagaagag agatgctcgg
    13801 tcaaataatt aatcgatgta atttctttga tgccgtcaat ccattttgtg ttgatttcat
    13861 ctgtcttttg aatgatatgc tgcatattaa aagcgcctat tccgccgaca agtaaattga
    13921 tcaggatcag tgtcaaaaat accaggctga atttgcggga aatcgtcagt cgcatataag
    13981 tggtctcctt taaaatcgta atgtgtgtgg atgtactata tatcggctga gaaatcgttt
    14041 ttttacctgg aaaatgaaaa ataaaaaacg gctcttttct cgcctttgag atcagaac
//
