LOCUS       BGC0000508              8700 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  S.epidermidis genes epiY', epiY, epiA, epiB, epiC, epiD, epiQ, epiP.
ACCESSION   BGC0000508
VERSION     BGC0000508
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
COMMENT     On Jun 21, 2005 this sequence version replaced S82283.1.
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:45:42
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8700
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     gene            complement(<1..827)
                     /gene="epiY'"
     CDS             complement(<1..827)
                     /codon_start=1
                     /db_xref="GOA:P30193"
                     /db_xref="InterPro:IPR001140"
                     /db_xref="InterPro:IPR004881"
                     /db_xref="InterPro:IPR011527"
                     /db_xref="InterPro:IPR017940"
                     /db_xref="UniProtKB/Swiss-Prot:P30193"
                     /gene="epiY_"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 70.2; E-value:
                     2.4e-21)"
                     /gene_kind="transport"
                     /note="Protein sequence is in conflict with the conceptual
                     translation; none of previously described start codons for
                     Staphylococci (ATG,TTg,GTG) is present"
                     /protein_id="CAA44250.1"
                     /transl_table=11
                     /translation="MLFSVIVSLIFLYIINKTLTLYLVCTLPILIIVILPIGNIMKRVS
                     SKSQEATAKLSSYYSNRLSTIKLIKTLSTYNIEKIKNYTLLKNIFDIELHKIKVLSFFE
                     PIMNLILFINIFGILFLGYYLMENNMMKSGDMFAYVLYLFQIINPIVSITSYWTEVQRA
                     IGSSDRILKINKEPEEVLTIKTTYNNFVQKMEINDLNFTKDNKQIINSISLDLHKGYIY
                     NIIGESGCGKSTLLNILAGLNTEYTGNIYLDKLDKSQFSKYEWRNLFSYITQDL"
     source          1..8700
                     /clone="pTepi14"
                     /db_xref="taxon:1282"
                     /mol_type="genomic DNA"
                     /organism="Staphylococcus epidermidis"
                     /strain="Tu 3298"
     protocluster    1..8700
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[1604:5808]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1605..5808
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..8700
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..8700
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000508"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..8700
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(781..1227)
                     /gene="epiY"
     CDS             complement(781..1227)
                     /codon_start=1
                     /db_xref="GOA:P30194"
                     /db_xref="InterPro:IPR001140"
                     /db_xref="InterPro:IPR011527"
                     /db_xref="InterPro:IPR017940"
                     /db_xref="UniProtKB/Swiss-Prot:P30194"
                     /gene="epiY"
                     /note="Protein sequence is in conflict with the conceptual
                     translation; none of previously described start codons for
                     Staphylococci (ATG,TTg,GTG) is present"
                     /protein_id="CAA44251.1"
                     /transl_table=11
                     /translation="MLKGDDIIKGLYDLWKITKPNTLLLSIGLIFSLIGTSFSLYIPLI
                     IRNALNKSSLSTDKIVIIIICFGLTLIFSGVSTYILGYIGQKIIQNIRSVTWNKVIKLP
                     YSFHLKNSASNLTSRLVNDTMNITRVFSVEFIFSYSITNIFIYN"
     gene            1381..1539
                     /gene="epiA"
     CDS             1381..1539
                     /codon_start=1
                     /db_xref="GOA:P08136"
                     /db_xref="InterPro:IPR006078"
                     /db_xref="InterPro:IPR006079"
                     /db_xref="PDB:1G5Q"
                     /db_xref="UniProtKB/Swiss-Prot:P08136"
                     /gene="epiA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gallidermin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_b"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="Epidermin prepeptide"
                     /protein_id="CAA44252.1"
                     /sec_met_domain="Gallidermin (E-value: 4.7e-40, bitscore:
                     125.0, seeds: 3, tool: rule-based-clusters)"
                     /sec_met_domain="mature_b (E-value: 7.5e-16, bitscore:
                     46.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEAVKEKNDLFNLDVKVNAKESNDSGAEPRIASKFICTPGCAKTG
                     SFNSYCC"
     CDS_motif       1381..1470
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MEAVKEKNDLFNLDVKVNAKESNDSGAEPR"
                     /locus_tag="epiA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       1471..1539
                     /prepeptide="core"
                     /locus_tag="epiA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="30.80"
                     /molecular_weight="2165.6"
                     /monoisotopic_mass="2164.0"
                     /core_sequence="IASKFICTPGCAKTGSFNSYCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEAVKEKNDLFNLDVKVNAKESNDSGAEPR"
                     /leader_location="[1380:1470](+)"
                     /alternative_weights="2183.6"
     gene            1605..4565
                     /gene="epiB"
     CDS             1605..4565
                     /codon_start=1
                     /db_xref="GOA:P30195"
                     /db_xref="InterPro:IPR006826"
                     /db_xref="InterPro:IPR006827"
                     /db_xref="UniProtKB/Swiss-Prot:P30195"
                     /function="modifying enzyme"
                     /gene="epiB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     898.1; E-value: 1.1e-271)"
                     /gene_kind="biosynthetic"
                     /protein_id="CAA44253.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.3e-124,
                     bitscore: 405.7, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 2.4e-15,
                     bitscore: 46.2, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 3.9e-19, bitscore:
                     58.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDNIFVPSNIYMVRTPIFSIELYNQFLKSDNIDYDLILQNDIFKE
                     SIMTTTYNLYQSIGKIDWEKDNKKTRNVKESLLKYLIRMSTRSTPYGMLSGVALGEFSE
                     NNNIKIKDSSFHKKDVKIDGQWLYKLVHYLESDYTYYKDSFVIWNQQNYIYNNRLYLDN
                     NSSITENKRNDVLSVKYNSILVFIHENSKKNITYEELVQLISSKYSIENKEEVKVFVQE
                     LINKEIIFSDLRPTLENKNPLDYIINSLNPKNSLVGTLINISNEITKYSKMPLGKGEYK
                     YLDIVNLMSQLFVSKNYLQIDTYIDYSRNELKQSLADNISEAAYILWLLSPNHFGTKTI
                     RNYHEFFMDKYGFEQLVNLKQLLSDINGFGYPKKDSYSFSNNIAFLKEKYLLAIQNNSH
                     IEITENDVKNLEKNNTVSKINAPVSTEIYSEIYFGNSIKGYEDFAVISPILGSFNAGAT
                     FGRFTGNFNIKKKNQLQKEIVHHYNNYMNENGLEISQLNEGPLNSRNVNILNNNRIYNT
                     CLNLNLPKSDIDINDIFIGATFNKLYLYSEKHDSRIVFVSNSMFNYEFGSELYKFLREI
                     SFEKTKFIQPITEEGIDSLPFCPRIIYKNIILKPATWKINSEMFSETENWLNRFATIRE
                     KWHIPKDVIIAFGDNRLLLNLLNDKHLIILKKELKKHGRIRILESFINESNNERMLEIV
                     TPLYKKTSLKEQSFIIPKNRNKHFNNLKDWFSIHLSIPKTYQDNFIQDYLLPFITELKV
                     NNFINKFFYIKFKEDEDFIKLRLLREDEDYSQIYSFIKNWKDYCLLNSELYDYSIVDYV
                     PEVYRYGGPHVIEDIENFFMYDSLLSINIIQSEFKIPKEFIVAISIDFLLDYLEINKSE
                     KEEILINNAEDLYRSNDIREYKNLLAKLTNPKNDYEILKKEFPNLHEFLFNKISILENL
                     KKTLQKSLYTSRSRIIGSFIHMRCNRIFGINPEKEKFVLSIFNEITKTKKYWDGCD"
     gene            4441..5808
                     /gene="epiC"
     CDS             4441..5808
                     /codon_start=1
                     /db_xref="InterPro:IPR007822"
                     /db_xref="InterPro:IPR020406"
                     /db_xref="UniProtKB/Swiss-Prot:P30196"
                     /function="modifying enzyme"
                     /gene="epiC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     376.7; E-value: 2.2e-114)"
                     /gene_kind="biosynthetic"
                     /protein_id="CAA44254.1"
                     /sec_met_domain="LANC_like (E-value: 1.5e-85, bitscore:
                     276.1, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVLYTCVVIEYSVLILKKKNLFYLFLMKLQKLKNIGMVVININN
                     IKKILENKITFLSDIEKATYIIENQSEYWDPYTLSHGYPGIILFLSASEKVFHKDLEKV
                     IHQYIRKLGPYLESGIDGFSLFSGLSGIGFALDIASDKQYSYQSILEQIDNLLVQYVFD
                     FLNNDALEVTPTNYDIIQGFSGIGRYLLNRISYNYNAKKALKHILNYFKTIHYSKDNWL
                     VSNEHQFLDIDKQNFPSGNINLGLAHGILGPLSLTALSKMNGIEIEGHEEFLQDFTSFL
                     LKPEFKNNNEWFDRYDILENYIPNYSVRNGWCYGDTGIMNTLLLSGKALNNEGLIKMSK
                     NILINIIDKNNDDLISPTFCHGLASHLTIIHQANKFFNLSQVSTYIDTIVRKIISHYSE
                     ESSFMFQDIEYSYGQKIYKNKVGILEGELGVLLALLDYIDTQNQSRKNWKNMFLIT"
     gene            5824..6369
                     /gene="epiD"
     CDS             5824..6369
                     /codon_start=1
                     /db_xref="GOA:P30197"
                     /db_xref="InterPro:IPR003382"
                     /db_xref="PDB:1G5Q"
                     /db_xref="PDB:1G63"
                     /db_xref="UniProtKB/Swiss-Prot:P30197"
                     /function="modifying enzyme"
                     /gene="epiD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Flavoprotein"
                     /gene_kind="biosynthetic-additional"
                     /protein_id="CAA44255.1"
                     /sec_met_domain="Flavoprotein (E-value: 3.8e-28, bitscore:
                     87.1, seeds: 143, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYGKLLICATASINVININHYIVELKQHFDEVNILFSPSSKNFIN
                     TDVLKLFCDNLYDEIKDPLLNHINIVENHEYILVLPASANTINKIANGICDNLLTTVCL
                     TGYQKLFIFPNMNIRMWGNPFLQKNIDLLKNNDVKVYSPDMNKSFEISSGRYKNNITMP
                     NIENVLNFVLNNEKRPLD"
     gene            complement(6366..6983)
                     /gene="epiQ"
     CDS             complement(6366..6983)
                     /codon_start=1
                     /db_xref="GOA:P30198"
                     /db_xref="InterPro:IPR001867"
                     /db_xref="InterPro:IPR011991"
                     /db_xref="InterPro:IPR016032"
                     /db_xref="UniProtKB/Swiss-Prot:P30198"
                     /function="response regulator"
                     /gene="epiQ"
                     /protein_id="CAA44256.1"
                     /transl_table=11
                     /translation="MISINIVGEVDSILIESILELDRRITINSSNIDPNFIIVYEKFDE
                     YYTFLKEFIGKIPIVIITGNTSYSRKCYFYSLGIDLYIIKNNESKSLILCRILNEIKKY
                     IKYVNDDFIDFENHQFVFNNYLVNLSNIELKILRCLYINLGRYVSKEELKKGVWDTEDF
                     VDSNTINVYIHRLRDSLKNCKEIEIINERKLGYKILIRKDLC"
     gene            complement(6994..8379)
                     /gene="epiP"
     CDS             complement(6994..8379)
                     /codon_start=1
                     /db_xref="GOA:P30199"
                     /db_xref="InterPro:IPR000209"
                     /db_xref="InterPro:IPR008357"
                     /db_xref="InterPro:IPR015500"
                     /db_xref="InterPro:IPR022398"
                     /db_xref="UniProtKB/Swiss-Prot:P30199"
                     /function="serine protease"
                     /gene="epiP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 254.6; E-value: 3.7e-77)"
                     /gene_kind="biosynthetic-additional"
                     /protein_id="CAA44257.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 5.1e-50, bitscore:
                     159.4, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNKFKFFIVFLILSLVFLQNEYAFGSSLNEELSYYSVEYDNAKTF
                     KESIKQKNIELTYKIPELHTAQIKTSKSKLNSLIKSNKNVKFVNPTCSTCVVEKSVKTG
                     KNLNNKKNGSHDLFDRQWDMRKITNEGKSYKLSPDRKKAKVALVDSGVNSSHTDLKSIN
                     KIVNEVPKNGFRGSENDESGNKNFEEDKLNHGTLVAGQIGANGNLKGVNPGVEMNVYRV
                     FGSKKSEMLWVSKGIIDAANDDNDVINVSLGNYLIKDNQNKKKLRDDEKVDYDALQKAI
                     NYAQKKGSIVVAAVGNDGINVKKVKEINKKRNLNSKTSKKVYDSPANLNNVMTVGSIDD
                     NDYISEFSNYGNNFIDLMTIGGSYKLLDKYGKDAWLEKGYMQKQSVLSTSSNGRYIYQS
                     GTSLAAPKVSGALALEIDKYQLKDQPETAIELFKKKGIEKEKYMDKKHYGNGKLDVYKL
                     LKE"
ORIGIN
        1 agatcttgtg ttatataact aaacaaattt ctccattcgt atttagaaaa ttgactttta
       61 tcaagtttat ccaaatatat atttccagta tattctgtat ttaacccagc taatatattt
      121 aataatgtac tttttccaca cccactttca cctataatat tgtagatata acctttatga
      181 agatccaaac ttatagaatt tattatttgt ttattgtctt ttgtgaagtt caaatcattt
      241 atttccattt tttgaacaaa gttattgtaa gttgttttaa tagttaatac ctcttctggt
      301 tctttattta tttttaaaat tctatctgaa gatccaattg ctcgttgtac ttccgtccaa
      361 taagatgtaa tagatactat tggattaata atttgaaata aatataaaac ataagcaaac
      421 atatctccgc ttttcatcat attattttcc attaagtaat aacccaaaaa taaaatacca
      481 aaaatgttaa taaatagaat taagttcata attggttcga aaaaagataa tactttgatc
      541 ttatgtaact ctatatcgaa tatatttttt aatagggtat agttttttat tttttcgata
      601 ttatatgtac ttaaagtttt tattaatttt attgtagata atctattact ataataagaa
      661 gataatttag cagtagcttc ttgagattta cttgatactc ttttcattat atttcctata
      721 ggtagtatta caattatcaa tataggtaat gtacacacta aatataatgt caaggttttg
      781 ttaattatat ataaaaatat tagtgatact ataactgaaa ataaattcta cagaaaaaac
      841 tctagttatg ttcatagtat cgtttactaa cctactagtt aagttacttg ctgagttttt
      901 taagtgaaaa ctataaggta actttatcac tttattccat gtaacacttc taatgttttg
      961 tattattttt tgacctatat atccaagaat ataagtagaa acaccagaaa atattaaagt
     1021 cagaccaaaa catataataa tgattacaat tttatctgtt gataagctag atttgtttaa
     1081 ggcatttcta attattaaag gaatgtataa tgaaaaacta gttccaatca aactaaatat
     1141 tagtccaata cttaaaagta gagtgttagg tttggttatt ttccataaat catatagacc
     1201 tttgataata tcatcacctt ttaaacttta tatcattaat ataatgttta ggaaaagtag
     1261 aagaaaatta cacttttgta attttctgaa tatacatagt atttattttg ggggagtact
     1321 aaaataataa ttgaaaaggg ttttataatc ctttttaata aatttttagg agtgtttaaa
     1381 atggaagcag taaaagaaaa aaatgatctt tttaatcttg atgttaaagt taatgcaaaa
     1441 gaatctaacg attcaggagc tgaaccaaga attgctagta aatttatatg tactcctgga
     1501 tgtgcaaaaa caggtagttt taacagttat tgttgttaat tcagaagaat tagattggca
     1561 gggcttcaat agaggctctg tcttaatttt gaggtgaaat agaattggat aatatatttg
     1621 ttccatcgaa tatatatatg gtaagaactc ctatattttc aattgaatta tataatcaat
     1681 tcttaaaatc tgacaatata gattatgact taattttaca aaacgatatt tttaaagaat
     1741 ctataatgac aacgacatat aatctttatc aaagtattgg caaaatagac tgggaaaagg
     1801 ataataaaaa aaccagaaat gtaaaagaaa gtttattaaa atatctcata agaatgagta
     1861 ctagaagtac accatatgga atgctaagcg gtgtagcttt aggggaattt agtgaaaata
     1921 ataatattaa aattaaggac tcttcgtttc ataaaaaaga tgtaaaaata gatgggcaat
     1981 ggttatataa attagtccat tatttagaaa gcgattacac atattataaa gacagttttg
     2041 tcatatggaa tcaacaaaat tatatttata acaatcgttt atatttagat aataattcat
     2101 caatcactga aaataaaaga aatgatgtat tatctgtcaa atacaattct atattagtgt
     2161 ttatacatga gaattctaaa aaaaatatta cttatgaaga acttgtacaa ttgatatcta
     2221 gtaagtacag tatagaaaat aaagaagaag taaaagtatt tgttcaagaa ctcataaata
     2281 aagaaattat attttctgat ttgagaccta cattagagaa taaaaatcct ttagattaca
     2341 ttattaatag tttaaatcca aaaaatagtt tagttggaac acttattaat atttctaatg
     2401 aaattacaaa atattctaaa atgcctttag gaaaaggaga atataaatat ttagatattg
     2461 ttaatttaat gtcacaatta tttgtttcta aaaactattt gcaaatagat acctatatag
     2521 attattcaag aaatgaatta aaacaaagtt tagctgataa tattagtgaa gcagcatata
     2581 ttctctggtt attatctcct aatcattttg gtacaaaaac tattaggaat tatcacgaat
     2641 tttttatgga taaatatgga tttgaacaac tagtaaattt aaagcaattg ctctcagata
     2701 taaatggatt tggctatccc aaaaaagaca gttatagttt ttctaataac attgcatttt
     2761 taaaagaaaa gtatttgctt gcaattcaaa ataacagcca tattgaaata acagaaaacg
     2821 acgttaaaaa tttagaaaag aataatacag tttctaaaat caatgcgcct gtttcaactg
     2881 aaatatatag tgagatatat tttggaaatt caataaaagg ttatgaggat tttgccgtga
     2941 taagtccaat attaggatct tttaatgccg gtgcaacttt tggaaggttt acgggaaatt
     3001 tcaatataaa gaaaaaaaat caattacaaa aagaaatagt gcatcattac aataattaca
     3061 tgaatgaaaa tggtttagaa ataagccaat taaatgaagg tcctcttaac tcaagaaatg
     3121 taaatatttt gaataataat agaatatata atacttgttt aaatttaaat ttacctaaaa
     3181 gtgatataga tataaatgac atatttattg gagctacatt taacaaactt tatctatatt
     3241 ctgaaaaaca tgattcaaga attgtattcg tatctaattc aatgtttaat tatgagtttg
     3301 gatctgaatt atacaaattt ttaagagaaa tttcatttga aaaaacaaaa tttatacaac
     3361 ctataactga agaaggcatt gactcattac ctttttgtcc aagaattatt tataaaaata
     3421 ttattttaaa accagctact tggaaaataa attcagaaat gttttctgaa actgaaaatt
     3481 ggttaaatag gttcgcaact attagagaaa aatggcatat tccaaaagat gtaattattg
     3541 cttttggaga taatcgattg ctattaaatt tattaaatga caagcatctc attatactaa
     3601 aaaaagaact aaaaaaacat ggtaggattc gaatattaga aagctttatc aatgaatcta
     3661 ataatgagag aatgttagaa attgttacgc cattatataa aaaaactagt ttaaaagaac
     3721 aatctttcat tatacctaaa aatagaaata agcacttcaa taatcttaaa gattggtttt
     3781 caattcattt aagtattcct aaaacatacc aagataattt tattcaagat tatctattac
     3841 catttataac ggaattaaaa gttaataatt ttattaataa atttttttac ataaaattta
     3901 aagaagatga agattttata aaattaagat tattaagaga agatgaagat tattctcaaa
     3961 tttattcttt cataaaaaat tggaaagatt attgcttatt aaatagtgaa ttatatgact
     4021 attctatagt tgattatgtt cctgaagtat atagatatgg tggtccacac gtaattgaag
     4081 atattgagaa tttttttatg tatgatagtc tattatcaat aaatataata caatcagagt
     4141 tcaaaattcc aaaagaattt atcgttgcta tatcaataga ttttttatta gattatttag
     4201 aaattaataa aagtgagaaa gaagaaattt taattaataa tgcggaagat ttatatcgta
     4261 gtaatgacat aagagaatat aaaaatttat tagctaaact taccaatcct aaaaatgact
     4321 atgaaatttt aaaaaaagaa tttccgaatc ttcatgaatt tctatttaat aaaattagta
     4381 ttttagaaaa tcttaaaaag acactacaaa aaagcttata tacttcacgt tctaggataa
     4441 ttggcagttt tatacacatg cgttgtaata gaatattcgg tattaatcct gaaaaagaaa
     4501 aatttgtttt atctattttt aatgaaatta caaaaactaa aaaatattgg gatggttgtg
     4561 attaatatta ataacattaa aaaaatttta gaaaataaaa tcaccttttt gtctgacatt
     4621 gaaaaagcta catatattat agaaaatcaa agtgagtatt gggatcctta tactctatct
     4681 catggttatc caggtataat acttttttta agcgcatcag aaaaagtatt tcataaagat
     4741 ttagaaaaag taatacatca atatattaga aaactaggcc cttatttaga aagtggtatt
     4801 gatggatttt cactttttag tggtctttcc ggaattggat ttgcgctaga cattgcgtct
     4861 gataaacagt actcttatca aagtatctta gaacaaattg ataatttact tgttcaatat
     4921 gtttttgatt ttttaaataa cgatgcattg gaagtaaccc ctactaacta tgatataata
     4981 caaggatttt ctggtatagg aaggtacttg ttaaatagaa tatcgtataa ttataatgca
     5041 aaaaaagcat taaagcatat acttaattac ttcaaaacaa ttcattactc taaagacaat
     5101 tggttagttt caaatgaaca tcaattttta gatatagata agcaaaattt tccgtcagga
     5161 aatataaatt taggattagc gcatggtatt ttaggtcctc tatcattaac agctttgagt
     5221 aaaatgaatg ggattgaaat cgaaggccat gaagagtttt tacaagactt cacttcattt
     5281 ttgctcaaac ctgaattcaa aaataataat gaatggttcg atcgctatga tatattagaa
     5341 aattatatac ctaattattc cgtcagaaac ggttggtgtt acggtgatac agggattatg
     5401 aatacattac ttttgtctgg taaagcctta aataatgaag gcttaattaa aatgtctaaa
     5461 aatattttaa ttaacataat agataagaat aatgatgatt taatcagtcc aaccttctgt
     5521 cacggactag catcgcactt aaccattatt catcaagcga ataaattctt taatctatct
     5581 caagtaagca catatatcga taccattgtc agaaaaatta ttagtcatta ttctgaagaa
     5641 agtagtttta tgttccaaga catagagtac tcatacggac aaaaaattta taaaaacaaa
     5701 gtgggaattc tagagggtga attaggtgtt cttttagctt tactagatta tattgataca
     5761 caaaaccaat caaggaaaaa ttggaaaaat atgtttttaa taacataata ggaggaataa
     5821 gatatgtatg gaaaattatt gatatgcgct acagcatcga taaatgtaat taatattaat
     5881 cactacatag ttgagttaaa gcaacatttt gatgaagtta atatattatt tagtcctagt
     5941 agtaaaaatt ttataaatac tgatgttctc aagttatttt gtgataactt gtacgatgaa
     6001 attaaagatc ctcttttaaa tcatatcaat attgtagaaa atcatgaata tattttagta
     6061 ttacctgcat cagcaaatac tattaataaa atagctaatg gtatatgtga taatctttta
     6121 actactgtat gtttaaccgg atatcaaaaa ttatttatat ttccaaatat gaacataaga
     6181 atgtggggaa atccattttt acaaaaaaat attgatttac ttaaaaataa tgatgtgaaa
     6241 gtgtattccc ctgatatgaa taaatcattc gaaatatcta gtggccgtta caaaaacaat
     6301 atcacaatgc ctaatattga aaatgtacta aattttgtat taaataacga aaaaagacct
     6361 ttggattaac aaaggtcttt tctaattaaa attttatatc cgagtttacg ttcattaata
     6421 atttctatct ctttacaatt ttttaaacta tcccttaatc gatggatata tacatttatt
     6481 gtattagaat caacaaagtc ttctgtatcc cacactccct tttttaattc ctcttttgat
     6541 acatatcttc caagattaat atataagcac cgtagaattt ttaattctat attagaaaga
     6601 ttaactaagt aattattaaa cacaaattga tggttttcaa agtctataaa atcatcatta
     6661 acatatttaa tatacttttt tatttcattt aaaattctac ataatattaa acttttgctt
     6721 tcattatttt ttataatata taaatctatg cctaaactat aaaaataaca cttcctacta
     6781 tagctagtat tacctgttat tataactatt ggaatttttc ctataaattc ttttaaaaac
     6841 gtataatact catcaaactt ttcatacaca attataaaat ttgggtctat atttgaagaa
     6901 ttaattgtaa ttcttctatc taattctaaa atactttcaa taagaataga atctacctca
     6961 ccgacaatat taatagaaat cattttattc ccttcattct ttaagtaatt tgtatacgtc
     7021 tagttttcca ttaccataat gttttttatc catatatttt tctttttcta tccctttttt
     7081 cttaaataac tctatagctg tttcgggttg gtcttttaat tgatacttat caatttctag
     7141 tgctaaagct ccagaaacct tgggtgcagc aagtgatgtc cctgattgat atatgtatct
     7201 tccattagaa gaagtactta aaacactttg tttttgcata tatccttttt ctaaccaagc
     7261 atcttttcca tacttatcta aaagtttata agaacctcct atcgtcatta aatctataaa
     7321 attatttcca taattagaaa actcagaaat ataatcatta tcatcgatgg atcctacagt
     7381 cataacatta tttagatttg ctgggctatc atataccttt tttgatgttt tagaatttag
     7441 atttcttttt ttatttattt cttttacttt ttttacattg ataccgtcat tacccacagc
     7501 tgcaacaaca atactacctt ttttttgagc atagtttata gctttctgta gtgcatcgta
     7561 atcaactttt tcatcatctc ttaatttttt tttattttga ttatctttaa ttaaataatt
     7621 tcctaaacta acgttgatta catcattgtc atcatttgct gcatcaataa ttcctttaga
     7681 tacccaaagc atttcacttt tctttgagcc aaatactcgg tatacattca tctctactcc
     7741 agggtttaca ccttttaaat taccgtttgc tcctatttgt cctgctacta atgtaccatg
     7801 attcaattta tcttcttcaa aatttttatt tcctgattca tcgttttcgc tacctctaaa
     7861 accattttta ggcacttcat taactatctt atttatactc tttaaatctg tatgactact
     7921 attcacacca gaatctacta aagcaacttt tgcttttttt ctatctggac ttagcttata
     7981 acttttacct tcatttgtta tttttcgcat atcccattgt ctgtcaaata aatcatggct
     8041 gccatttttt ttattattta aattttttcc tgtctttaca gatttttcaa ctacacaagt
     8101 ggaacaggta ggatttacaa acttgacgtt tttattactc tttattagtg aatttaattt
     8161 tgatttgcta gttttaattt gtgctgtatg tagttcagga attttataag ttaactcgat
     8221 atttttttgt ttaatggatt ctttaaaagt ttttgcatta tcatattcaa cactataata
     8281 acttaattct tcatttagtg aacttccaaa agcatactca ttttgcaaaa aaactaatga
     8341 caatattaaa aaaacaatga aaaatttaaa tttgttcata tagcacctct aacatattat
     8401 ttatattaaa cattaattta acacttatgt ttttactttt ttatttatat tatctttaat
     8461 aatgttctgt tgcaagatga aaaatacgag gtatcaaagt accgatacag cgagtattac
     8521 actcaattaa ttaaaaataa aatatgttgt gattaaaatt tattttataa aagtatgggc
     8581 aatttattat tattcaagtt aaaacaaaga gtccgggaca taaagtttca gcctcttcgt
     8641 cctaattacc aaaaaactta ctccaaaatc cttttttaga ttggtttttt ctaatttttt
//
