LOCUS       BGC0000509             15487 bp    DNA     linear   BCT 11-OCT-2012
DEFINITION  Staphylococcus epidermidis Orf1, ElxO (elxO), ElxT (elxT), ElxA
            (elxA), ElxP (elxP), ElxB (elxB), ElxC (elxC), Elx1 (Elx1), Elx2
            (elx2), Elx3 (elx3), and OrdA (orfA) genes, complete cds.
ACCESSION   BGC0000509
VERSION     BGC0000509
KEYWORDS    .
SOURCE      Staphylococcus epidermidis
  ORGANISM  Staphylococcus epidermidis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:45:51
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..15487
                     /db_xref="taxon:1282"
                     /mol_type="genomic DNA"
                     /organism="Staphylococcus epidermidis"
                     /strain="15X154"
     protocluster    1..15487
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[8714:12901]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      8715..12901
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..15487
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..15487
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000509"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..15487
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     CDS             4196..4729
                     /codon_start=1
                     /note="ORF1"
                     /product="recombinase"
                     /protein_id="AFN69429.1"
                     /transl_table=11
                     /translation="MIVGYARVSSIDQNLERQLENLKTFGAEKIFIEKQSGKSVENRPI
                     FQEALNFLRRGDRFVVEAIDRLGRNYDEVIGNPLLDKFMKNIIIQILAMISEQERNESK
                     RRQAQGIKVAKEKGVYRGRPLLYSVNAKDPQKRIIYHRVVEMLEEGQAISKIAQEVNIT
                     RQTVYRIQRDNLLS"
     gene            complement(4943..5689)
                     /gene="elxO"
     CDS             complement(4943..5689)
                     /NRPS_PKS="Domain: PKS_KR (4-148). E-value: 9.7e-10. Score:
                     30.5. Matches aSDomain: nrpspksdomains_elxO_PKS_KR.1"
                     /codon_start=1
                     /gene="elxO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001:short-chain dehydrogenase/reductase SDR (Score:
                     205.9; E-value: 1.2e-62)"
                     /gene_kind="biosynthetic-additional"
                     /note="oxidoreductase"
                     /product="ElxO"
                     /protein_id="AFN69430.1"
                     /sec_met_domain="adh_short (E-value: 5.4e-36, bitscore:
                     113.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 1.2e-33, bitscore:
                     106.6, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKNVLITGGFKGIGKQVALEFLKNDYHVCITSRYFEKEKRIPHL
                     FSSYEENISFYQLDVTDEEQVNEIINKIVKKFGRLDVLVNNAGISLSDGLLTETKTTDF
                     NKMINTNILGTYFCMKYALKHMQKVSCGAIVNISSITGLSGFPYSILYGSTKHAVIGLT
                     KGAAVEFADKGIKINAVAPGIIKTETLQKEIDSGEFSEDSISSIHPMQKLGTTLDVAKG
                     IYFLANEDNNFITGHVLSIDGGYLSQ"
     aSDomain        complement(5246..5677)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_elxO_PKS_KR.1"
                     /evalue="9.70E-10"
                     /label="elxO_PKS_KR.1"
                     /locus_tag="elxO"
                     /protein_end="148"
                     /protein_start="4"
                     /score="30.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VLITGGFKGIGKQVALEFLKNDYHVCITSRYFEKEKRIPHLFSSY
                     EENISFYQLDVTDEEQVNEIINKIVKKFGRLDVLVNNAGISLSDGLLTETKTTDFNKMI
                     NTNILGTYFCMKYALKHMQKVSCGAIVNISSITGLSGFPY"
     CDS_motif       complement(5591..5674)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_elxO_0001"
                     /evalue="1.60E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="elxO"
                     /protein_end="33"
                     /protein_start="5"
                     /score="14.3"
                     /tool="antismash"
                     /translation="LITGGFKGIGKQVALEFLKNDYHVCITS"
     gene            complement(5708..7429)
                     /gene="elxT"
     CDS             complement(5708..7429)
                     /codon_start=1
                     /gene="elxT"
                     /gene_functions="resistance (resist) ABC_efflux (Score:
                     257.2; E-value: 1.9e-78)"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 330.7; E-value:
                     3e-100)"
                     /gene_kind="transport"
                     /note="ABC transporter"
                     /product="ElxT"
                     /protein_id="AFN69431.1"
                     /transl_table=11
                     /translation="MKTIHNNPLFYLFKKIRWPKKIFFTAIIITSLGSLSELAVPLLTG
                     KFIDILVSNGINYKFISLLTLVFILDALLNGVGMFLLIKAGEKIIYSIRLLLWNKIIYL
                     EVPFFDKNDSGQLISRLTDDTALINNFISRKIPSVIPEMLTLLGSLIMLFVMDWKMTLL
                     TFIIIPLFLLVIIPLSNIIESLSQSTQLEIAKFTGIINRALSAIRLVKISNTENKELIT
                     AEKKLNAIYRLNIRHARITAILEPFSNILLIIMIGIILGFGGYRISTGVITSGTLVTMI
                     FYVVQLSSPITSLSTLLTDYKNAKGATKRIFEILKEQQESFNGIPYNKKQSYDLSFSSV
                     YFSYNKKHILKDISFNIPKNKTTAIVGPSGAGKTTIFSLITRMYKIDSGAIKVGNESIY
                     NFNLAEWRENIGYVMQNNSMISGTIKENILYGIKQDVSKDVFEKYVRFSNSHNFIMSLE
                     SQYNSEVGESGNKLSGGQKQRINIARNLIKDPEILLLDEATSSLDSDSETKIQKSLNFL
                     SKNRTTIIIAHRLSTIKDADQIIFLDNGKITGIGKHTFLMETHPKYKKFVLNQKLS"
     gene            7589..7756
                     /gene="elxA"
     CDS             7589..7756
                     /codon_start=1
                     /gene="elxA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Antimicr18"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="precursor peptide"
                     /product="ElxA"
                     /protein_id="AFN69432.1"
                     /sec_met_domain="Antimicr18 (E-value: 1.7e-09, bitscore:
                     27.4, seeds: 2, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKELFDLNLNKDIEAQKSDLNPQSASIVKTTIKASKKLCRGFTL
                     TCGCHFTGKK"
     CDS_motif       7589..7660
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MKKELFDLNLNKDIEAQKSDLNPQ"
                     /locus_tag="elxA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       7661..7756
                     /prepeptide="core"
                     /locus_tag="elxA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="18.10"
                     /molecular_weight="3173.8"
                     /monoisotopic_mass="3171.8"
                     /core_sequence="SASIVKTTIKASKKLCRGFTLTCGCHFTGKK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MKKELFDLNLNKDIEAQKSDLNPQ"
                     /leader_location="[7588:7660](+)"
                     /alternative_weights="3191.8"
                     /alternative_weights="3209.9"
                     /alternative_weights="3227.9"
                     /alternative_weights="3245.9"
                     /alternative_weights="3263.9"
     gene            7793..8728
                     /gene="elxP"
     CDS             7793..8728
                     /codon_start=1
                     /gene="elxP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 135.6; E-value: 4.8e-41)"
                     /gene_kind="biosynthetic-additional"
                     /note="protease"
                     /product="ElxP"
                     /protein_id="AFN69433.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.9e-20, bitscore:
                     62.8, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFLYLPFIIQLGDFMDNFLSWPNKNKYFDEIKDEVKILYIDSGCD
                     INHIEVKENILINESKSFVDNDSELYDYTGHGTQIISAITGKHNMIGLYPRSKIVIYKI
                     TNYKGETKFEWLYKALYKAIKMDYKIINISYSGYTQNNYIISKFKRLIEQAVKKNIHIL
                     CSASNDEVEKGFSIPSDFKGVYKIASINIEDKYSSYISKSNAEYFAPGGDNYLKTQNPQ
                     SFILLANSSISNFNIGSDFGIDKRYTLNFGNSIACSYVSCCIGLVVTRRKIKFNKDTSK
                     RYIDCLYNKYKHISLNVIKNTKEIITNEHI"
     gene            8715..11675
                     /gene="elxB"
     CDS             8715..11675
                     /codon_start=1
                     /gene="elxB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     559.8; E-value: 1.7e-169)"
                     /gene_kind="biosynthetic"
                     /note="lantipeptide dehydratase"
                     /product="ElxB"
                     /protein_id="AFN69434.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 3.2e-81, bitscore:
                     262.8, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 7.4e-10,
                     bitscore: 29.1, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 5.1e-20, bitscore:
                     61.7, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNIFKKYMYRSPLLSLNEFNKIQRDDLTDKEYTKYLINYVEENNL
                     YANIYSSSKVLYFALINFNNDTSDKKTKSILKSLYKYLVRMIFRATPFGMYSGVGINEI
                     YESNYIEKESVTYYGYLNNHLLYYLIDRLHNNSKVLHKLKLNINPILHHDKKNIFLPYQ
                     VDYSLTSIVSSDNISQRNNELIEKVINLAKNSIEFKELKEIIMYEFKANEEIAVNYLKK
                     LIKEDFLMTDFKINLSKKNAFKGILFKLQEIDEIDNEVYEILDNLSIIINQIKNTIDRN
                     SILELLIDADKIVKKFYPEFKENAINIDTKLQGKKINLTENDIDDIINVSTLISKLTVF
                     KSSKVLENYKNKFLEIYGENEDVQLLKLLNSSTGLGIPKEYNLSSNLNDLKKANALSGL
                     LENWKTEALIKNQDSIVLNNRRLKELKPYLLKDNINTSFDVFFIKFNKTSSKLYLKTNS
                     GSLQSMQTYGRFMYMFNKQLKYEVNEFCKFYVPMMSHKEIIYNHPNPKLQNVMSSTFSS
                     DAIDFLGVEGNLNIENLYVCLGEDFKFYIKDKQTGNIIFPDFKDMHNTNLSPVVIRFLS
                     DISLQYSTGGYFLNYSATEHAYSPRIEYKNVVLSPRKWHMNFPKKLNFDAFLNELKKFK
                     ELYNLDIVFYIINDDHKLYIDTRFDISLQILYDQYKKQEILEVEEVEEELSFNKDIGIN
                     ELVFSVSNNENSISEKNIAMIDKDIRNYKEVILPGNNWICLNLYYDEYNFKEFMNKGIW
                     NDLFKDIGKNHDIDTVFFIRYFDSDPHIRLRFRIKENINKNRNDILNKLNKFKNENFLK
                     TFSIVPYYRESYRYGGLNCIHLAEKCFQIDSKIVARYYGELDDKSDKIDFAIDNIIEIL
                     NLFTGKCIEENIKILSVFGKNKENKDLYREKRNRIFKSISNNDKYIYNYGISDFRKKVY
                     LKYINELKKENKLNNHDIILSIIHMFCNRLFGIDREIESKVLEIIYRSLIDYKKIN"
     gene            11693..12901
                     /gene="elxC"
     CDS             11693..12901
                     /codon_start=1
                     /gene="elxC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     223.6; E-value: 6.8e-68)"
                     /gene_kind="biosynthetic"
                     /note="lantipeptide cyclase"
                     /product="ElxC"
                     /protein_id="AFN69435.1"
                     /sec_met_domain="LANC_like (E-value: 9.1e-59, bitscore:
                     188.4, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENSIQKSLSYLSDIKLVNKNIEDNIGSDVIFKISLAYGYPGIAL
                     LFNEAYKLTKNIDYYEYCHNYLSETLNLIKHNPMYSTSLFTGVMGYLFTLASCSNDGEN
                     YRNISYQILDEYNAFFNVSLNEVNIALLNKDFSKDSFDIINGAAGTLLSLLHIYDIYGN
                     LHYNLEKWIQSLTNLLEKLIIKSISNNYPEHENVLTDLGVSHGITGIINIVNAAFKRDF
                     GSYTLHNTLLRAKNFLLSLIEKYESSYIIPNFRHFSKENLNHRDAWCYGTPGVAMVLYN
                     LGINLKDKEIINTSKKLASETLKRNKSTRLLISPTLCHGYSGIAIINKYLGNFYVEDYY
                     YNQIENSKNTNLRLMFRNIDYNGENFVYDDNLGLLEGSSGVLLTLLSKKGFNSTWYKLF
                     CFA"
     gene            12940..13158
                     /gene="elx1"
     CDS             12940..13158
                     /codon_start=1
                     /gene="elx1"
                     /note="hypothetical protein"
                     /product="Elx1"
                     /protein_id="AFN69436.1"
                     /transl_table=11
                     /translation="MIFLTKILDTLAYICVGLIFLKYLILTVNSCFDWHLRWYFLENIP
                     YMAIILFVATFIFAVPSEMIKDKLKDK"
     gene            13183..13908
                     /gene="elx2"
     CDS             13183..13908
                     /codon_start=1
                     /gene="elx2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /note="CAAX amino protease"
                     /product="Elx2"
                     /protein_id="AFN69437.1"
                     /sec_met_domain="Abi (E-value: 6.6e-14, bitscore: 41.8,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MILQLKSVNWPEFIFFPIVFFSSFLVNIILSLINNFFNINFFSVL
                     GTKNVVIMSDFSINLIIIFLFLILEKKTKKNIVESLEYIKKNIFLFMFLFTLPKLVIIL
                     INYLFNLSNVNFNLSKTENQMLIIDWIKEPNYFFMKIIIFLSIILVGPISEEILYRHLI
                     IGELGKIFSYKLMAFISIILFSLIHVSDAKSSLEILTYLILSLSLVLIYLISKRNIFVS
                     ITLHCFINITSYAYIYYFY"
     gene            13937..14152
                     /gene="elx3"
     CDS             13937..14152
                     /codon_start=1
                     /gene="elx3"
                     /note="hypothetical protein"
                     /product="Elx3"
                     /protein_id="AFN69438.1"
                     /transl_table=11
                     /translation="MTKILDFLSTACFVLLSSKYAISSINMIFDLKLRWYILEDIPHLS
                     IILLILTLVFFVSSEMIKDNKEKNKK"
     gene            complement(14476..15261)
                     /gene="orfA"
     CDS             complement(14476..15261)
                     /codon_start=1
                     /gene="orfA"
                     /note="membrane-spanning protein"
                     /product="OrdA"
                     /protein_id="AFN69439.1"
                     /transl_table=11
                     /translation="MKCKMGTLVKQECFKLFKKKSTYILPIIIIIIMLSLAILGNHYSD
                     VFNLKESFKGGFSSFSWVVFLMIIQASTIISMEFHYGTIKNLLYRNYSRTSIIVSKFIT
                     LLIYSLIIFLVFIIISLLLHLIFNGETSLTEHSKNHISLLNSMLLTALGTFVGMWLVMS
                     LTLLISCIMKSPGVSIAVGIVFYFAVSIISGLLTVVIDKWEWLKWNPLNMMNIMAQITD
                     NDTFKKITKLELHELFIGNIIYIVIFLVLVVVAFKKKNV"
ORIGIN
        1 gatcttatac atttatacct ccatatataa ctcatattat atggatttgt attttctata
       61 attatagtaa tcaaaatata ctatctagtt acgctgcatc tgctatcatt ttattcttta
      121 tctcttcttg gataacattt attatttttg atttggaggg aaataatgaa aagcatttat
      181 tatttataca attaaattct aaaattaaat ttctaaattt aaaaatattt ttttccttta
      241 tttcttcaat acctttaata attatttctt tccttttccc tattgttact tataggtttt
      301 ctgaagctat aactttccaa tacgcctttc taagtatata tttacatatt atcgtagtca
      361 tattaggtat tattctcgga tcactgattc gtatacaaac tatattgtct agaaagtata
      421 gttggttatt attagtgcta cttactttac tatcaattat taaatttata ttagttaaca
      481 atcaacatat tttaaaatac gttttaatta ttgtgccttc cgtaggggaa tttttaaact
      541 atttcaatca acctattgca aaattatggt gttttgactt tctaattctt aatactaata
      601 tagcggtata tatagtaata actcttattt taattaacct tatatttaaa tattcagaga
      661 atcattaatt aaccctatta ctaatttaaa atataattaa tatggttctg ttgcaaagtt
      721 gaaaaataaa agaagttatt gaacacatat aaatcaagta gacaaaaatg attatgacaa
      781 acgtattgaa catgaaaata aacttgataa taaaattaat cataaaaaaa gaagaagaaa
      841 cacgagtaag aacagaagag aaataactaa ataagaagtt agtaagatat tgaaaatata
      901 caagtttcat tctttagcaa taagaagctt aatcaaaagg gtacatttac tttgttttat
      961 tgaagaattg gaaggttaac gtcaataaag cactatttct ttataatcta ttttcagtag
     1021 aaaggaattt tttatgaata gtaattatat tacttttgct ttttttaatg aaataccgtt
     1081 gtttgatttc aaatttctat tgttaaatca tgagattgag caatggaacc aggaatatga
     1141 agcttttaat tttgaattta atggaattaa atttaaaagt agattggcta aaaaaactcc
     1201 taaaaaagag gggtattttg ttgcatgctg gcgtaaaaat gagactaata aaaatagacc
     1261 ttttaattgt aaagaatcaa aagataaatt gattattaat atattagaca gatctaaaaa
     1321 aggccaattt attttcccta aagagttgtt ggttaaaaaa gggattatta gttctgaaaa
     1381 acacaaaggt aaaatggcta ttcgcgttta tcctagttgg gaaaagaatt taaataaaac
     1441 agctcttaat acacagaaat ggcaaattcc ttatttttta gattttacta agggatttaa
     1501 tgagaaaaaa attaaggaat tgtatttaaa ttgactttaa ttttagaaaa gagtttcttc
     1561 ttgtactgat aattaatatg cttatattag acagtatcat atatcttcat tttcaataaa
     1621 catacaatta caaccataac ttttaagttt aagtaattgt aaatcaattg attcattttt
     1681 aggtaaatgg cgaatataac caatttttga cattagatta gctcctttgg aatagtttag
     1741 aaaatatgaa gttatctata ttttactaaa gtaaagttta ttatagttac tttttgatgt
     1801 ttatttagac ataaaatgac ttatcggaag ttatctgtaa ctcatctagt acagatgata
     1861 taatatacag gtcgcctatc tctcaggcgt caatttagag acgcagagag gaggtgcata
     1921 tcagatggtc gaagctcttg ttcacatcat gaccacagtc atcagtggtt gtattgttgc
     1981 gttatttacg cattggctac gtacacgtaa caataagaaa aaataggcga tattagccaa
     2041 cacctaaaaa tcccctcact actcgcaata gtgaggggat tggtgcatat aatgcacgaa
     2101 gctcttgtta tctaaattat aacactggtt agtagagtaa tgcaaaaatg tatataataa
     2161 atattttatc atgattcaaa gtattcgtta tttctataaa tattagaatt atgacttaat
     2221 gtctaaattg ttctaaatat tatttatact taacactgca agtgatactt ccaatcgcga
     2281 atacggcgtt actatagtta atacaataat acagatgata ttataatcca tacagtaact
     2341 acaataggaa ttaatgattt aaataaaaaa atgccatatt ttttctttct atacaagtcc
     2401 attattagcc agataactat aataatgatt ccaacaaaaa taaatacagg gttcatcgac
     2461 aaactgtcta ctttaatatc actaacaata tgtactttcg taagtattaa aagtacgata
     2521 gcaattaaaa agtagtaaac ggatttaatt ttcgaaattt gtaagtctaa cttttgatat
     2581 tctatatcat cttgatttgc attttccttt ttactaatta gatagttcaa aactttaggt
     2641 tttgtccaaa aattcttgat tgcaaaatac atatacacaa atgaaccaat taaagtaaat
     2701 gctatataat tacttaaatc atttcctgta agtttatttc caataaaatg aaaaataatc
     2761 aacattatta caataacaaa atctaaaaaa atagataaaa ttgaaaaatt ttcagatttc
     2821 agaagactgt caaatagagc taaataaaat ataataaata ctccaatcag tacaaaataa
     2881 accaatactt acacttcctt aattattctc atataccttt gcttttgttt cacatcaacc
     2941 tttatatcgt tcactattaa aacatttttt gatagagtag ggtcaacttt taacatatca
     3001 atatattcta tgtttaattc agagatatgt tgtaacctgt catttaaaga aataaacact
     3061 atattctgat attcattaat cccaataagc tccctttgaa atattatagg tcctgttagc
     3121 tatttatagt tataaattat aaaattcatt tactgtatct aaacctttta accagttttc
     3181 ttctttatta atttcaaatg ttattttgac gaaataaggc attacattca tacctaattc
     3241 tattataatt tcatcccatc ttctttaatg tcataattta agagacctaa ataaaaatat
     3301 gttcgtccaa ttaataaatg gctacaaaat aacattatca ttcccatcca aatcgttcta
     3361 ttttcttcag aatcaacagg aagaattaat gatacataca gcaatagtcc aaatgataat
     3421 aaaatagtta aaattccaat cattctaata aaactaccat tttggatacc aaacatctca
     3481 aattcaccta gacaaaataa gaatagacct atagcagaga ctattatgag cccacaaatt
     3541 aacaaccaat atgtaaaaat atcgtctcta gaatctgcaa aataaaaagc ataagcagaa
     3601 ctaattaaaa atggaatatt gtatcctgaa agtagattga tggtcatata tattacaact
     3661 ctgaatttat aattttttcg attataatgt ttaaaaatat actcacgttc ttgtaaataa
     3721 agaagcaacc ataataataa tgatcctatt aaatatactt tcatatataa aggcatattc
     3781 ccttttatac ctggacctgc atatataaaa tagtaaactg caaaaataat gaaattcact
     3841 aatgataatc ctattaaatg tgaatgttga attgattcta ttgatttttt ttgtagattt
     3901 acctccataa tatccacctt ccttttatca atataatatt atcacaactt atattaaata
     3961 tcgttgctat ttggattaat tgctttttat ctatattatc tattgatatt tttagtatta
     4021 ttatattttc agaaattctt tctaattggg atgacactac aaaacttaaa gctgctttaa
     4081 aaaaacaatt tatatataaa ttaagggtac accctaatca tacaaatgaa gttgttgaat
     4141 ttatagcaac taaaacgtat gattagggtg tatattaatt atgaggtgaa aatcaatgat
     4201 tgttggctat gcgagagtat catctatcga tcaaaattta gaaagacagc ttgaaaattt
     4261 aaagacgttt ggtgctgaaa aaatttttat agaaaaacag tctggtaagt ctgttgaaaa
     4321 tagaccgata tttcaagaag cacttaactt tttgagaagg ggggatcggt ttgtagtcga
     4381 agcaattgat cgtttaggtc gtaattacga tgaagtgatt ggcaatccat tattagataa
     4441 atttatgaaa aacataatca ttcaaatatt agcaatgatt tcagaacaag agcgaaatga
     4501 aagtaaacgt cgccaagcac aaggtattaa agttgcgaaa gaaaaaggtg tgtatagagg
     4561 acgcccttta ttatattctg tcaatgcgaa agatcctcaa aaacgtatca tttatcatag
     4621 agttgtagaa atgttagaag aaggccaagc aattagtaaa attgcccaag aagtaaatat
     4681 tacaagacaa acagtatata gaattcaacg cgacaattta ttatcttaat aaattgtcgc
     4741 gttgaattct atattgattt taagcctcat tgtatgttat tattcaaaat tagacacaga
     4801 ttaaataaga tttaaatcaa ataattcttt attgcacttc ccacattttt ctatatgtaa
     4861 tctaacaaag aaaaatgtgg gatttgtcat tcacaaaata cataaactca gtttttagta
     4921 taatataaaa agataaatgt atttattggg ataaatatcc tccgtcaata cttaatacat
     4981 gacctgtaat aaaattattg tcctcgtttg ctagaaagta tatcccttta gcaacatcta
     5041 aagttgttcc taatttttgc ataggatgta ttgaagaaat agagtcttct gaaaattcac
     5101 cagagtctat ctccttttgt aaagtttcag tttttataat tccaggagcc actgcgttta
     5161 ttttaatccc tttatcagca aattcaacag ctgctccttt agttaaacca ataacagcat
     5221 gttttgtgct accgtataat atagagtaag gaaatccgct taatcctgta atagaagaaa
     5281 tatttactat tgctccacaa gaaacttttt gcatatgttt taatgcatac ttcatacaaa
     5341 agtaagtgcc taaaatattt gtatttatca ttttattaaa gtctgtagtt ttagtttctg
     5401 ttaataatcc atcacttaaa gaaataccgg cattgtttac taaaacatct aatcttccaa
     5461 atttttttac tatcttattt attatttcgt ttacttgttc ttcgtctgta acatctaatt
     5521 gataaaaaga aatgttttct tcatatgatg aaaataaatg aggaattctc ttttcctttt
     5581 caaaatacct gcttgttata catacatgat aatcgttttt taaaaattct aaagctactt
     5641 gttttccaat tcctttaaag ccgcctgtaa taagaacatt ttttttcaca ataattttcc
     5701 tttctcatca tgataatttt tgatttaaaa caaatttttt atattttgga tgagtttcca
     5761 ttaaaaaggt atgtttacca atgcctgtta tttttccgtt atctaaaaat ataatttggt
     5821 ctgcatcttt aattgtagac aatcgatgtg caataataat tgtagttcta tttttagata
     5881 gaaagtttaa agacttctgt atctttgttt cactatcact atcaaggcta gatgttgctt
     5941 catctagaag taaaatttca ggatctttaa ttaaatttct agctatattg attctttgtt
     6001 tttggcctcc agatagtttg ttgccacttt ctcccacctc tgaattatat tggctttcaa
     6061 gggacattat aaagttatga gaattagaaa atcggacata cttttcgaaa acatctttcg
     6121 aaacatcctg tttaataccg tataagatat tttctttaat tgttccactt atcatagaat
     6181 tattctgcat cacataacct atattttcac gccattctgc cagattaaag ttgtaaatag
     6241 actcatttcc aactttaata gctccactat caattttata catcctagta atcaagctaa
     6301 atatagttgt tttaccagct cctgagggtc ctactattgc tgttgtttta tttttaggta
     6361 tattgaaaga tatatctttc aatatatgtt tcttattata agaaaagtaa actgagctga
     6421 aagataaatc gtatgattgt tttttattat atggaatacc attaaaggat tcttgttgtt
     6481 ctttcaaaat ttcaaagatt cttttagtag ctcccttggc atttttatag tcagttagta
     6541 atgtggacaa gctagttatc ggagaactaa gttgcactac ataaaaaatc atggttacta
     6601 gtgtaccaga agttataaca cctgtagaaa ttctataacc tccaaagcct aaaataatac
     6661 caatcataat tattaataaa atattagaaa aaggttctaa tattgcagtt attctagcat
     6721 gtctaatatt taaacgataa atagcattta gctttttttc agcagttatc aattctttat
     6781 tctctgtatt tgatatttta actaatctta ttgctgacaa cgccctatta attataccag
     6841 tgaattttgc aatttctagt tgagtagatt gagataagga ctcaataatg ttactaagtg
     6901 gaattattac tagtaaaaat aaagggatta ttataaaagt taaaagagtc attttccaat
     6961 ccattacaaa caacattatt aatgaaccta ataaagttaa catttctggt ataactgagg
     7021 ggattttacg tgaaataaaa ttatttatta gtgcagtatc gtcggttaat cgacttatta
     7081 attgtccgct atcattttta tcgaaaaagg gtacttcaag ataaataatt ttattccaca
     7141 atagtaatct tatagaataa attatctttt ctcctgcttt aatcaataaa aacattccca
     7201 ctccatttaa taatgcatct aatataaaca ctaaagttaa tagtgagata aatttataat
     7261 ttataccatt acttacaaga atatctataa acttaccggt taataaaggg acagctagtt
     7321 cactcaaact acctaacgaa gttattataa tagcagtgaa aaatattttt ttaggccacc
     7381 taattttttt aaataaatag aaaagagggt tattatgaat cgttttcata ggaaatcctt
     7441 ttttttttta aattcaatta aataataaca taaaacaaga aaagcatata tatttatata
     7501 tgagtataca caataattta atttttatta aaagtattga taatagtaat gcgataaatt
     7561 atattaggat tataggaggt gagatattat gaaaaaagaa ttatttgatt taaatcttaa
     7621 taaagatatc gaggcacaaa aaagtgacct aaatccgcaa tcagctagta ttgttaaaac
     7681 aactatcaaa gcttctaaaa agctttgtag aggatttaca ctaacttgtg gatgtcactt
     7741 tactggtaaa aaataatagg tgtttttagg taggtataaa agtatgttta tcatgttttt
     7801 atatctacct tttataatac agttaggaga ttttatggat aattttctta gttggcctaa
     7861 taaaaataaa tattttgatg aaataaaaga tgaagttaaa atattatata tagatagtgg
     7921 gtgtgatata aatcatatcg aagttaaaga aaatatattg ataaatgaat ctaaatcttt
     7981 tgtggacaat gatagtgaat tatatgacta tacgggacat ggaacacaaa ttattagtgc
     8041 aataacaggt aagcataata tgattggact atatcctaga agtaaaattg taatatataa
     8101 aataactaat tataaaggtg aaactaaatt tgaatggtta tataaagcat tatataaagc
     8161 tataaaaatg gactataaaa ttattaacat aagttattca ggatacaccc aaaataatta
     8221 cataatatct aaattcaaaa gattaataga acaagcagtt aaaaaaaata tacatatttt
     8281 atgtagtgct agtaatgatg aagtggaaaa aggtttttca ataccttctg attttaaagg
     8341 agtctataaa attgcgagta taaatattga agataaatat tctagttata tttctaaatc
     8401 taatgctgaa tactttgctc ctggaggaga taattattta aagacacaga atccacaatc
     8461 atttattttg ttagctaata gttctatttc taactttaat attggttctg attttggtat
     8521 agataaaagg tatactttaa attttggtaa tagtattgca tgctcctatg tttcttgttg
     8581 tattgggcta gtagtaacac gaagaaaaat taaatttaac aaagatactt ctaaaaggta
     8641 tatagattgt ttatataata aatacaagca tataagtttg aatgtaatca aaaacacaaa
     8701 ggagattatt actaatgaac atatttaaaa aatatatgta taggagtcct ttattatcac
     8761 taaatgagtt taataaaatt cagagggacg atcttacgga caaggaatac actaaatatt
     8821 taataaacta tgtagaggaa aacaatcttt atgcaaatat atactcatct agtaaagttt
     8881 tatattttgc tttaataaac tttaacaacg atacttctga taaaaaaaca aaaagtattt
     8941 tgaaatcttt atataaatat ctagtgagaa tgatttttag ggctacacct tttggtatgt
     9001 actctggggt agggattaat gaaatatatg aatctaacta tatagaaaaa gaaagtgtaa
     9061 catattatgg ttatttaaat aatcacttat tatattattt gattgatcga cttcataata
     9121 attcgaaagt tttacataaa ttaaagttaa acataaaccc cattttgcat catgacaaaa
     9181 aaaatatttt tctaccttat caagtagatt attctctaac ttcaatagta tcttctgata
     9241 atattagtca aagaaataat gagttaattg aaaaggttat aaatctggct aaaaattcta
     9301 ttgagtttaa agaattaaaa gaaatcatta tgtatgaatt taaagctaac gaagaaatcg
     9361 ctgtaaacta tttgaaaaaa ttaataaaag aagatttttt gatgactgat tttaaaatta
     9421 atttaagtaa aaaaaatgct tttaaaggca tattatttaa gctacaagaa attgatgaaa
     9481 tagacaatga agtatatgaa attttagaca atttatcaat cattataaat caaataaaaa
     9541 acacaataga tagaaatagt atactagaat tactaattga tgcagataaa atagtaaaaa
     9601 aattttatcc tgaatttaaa gaaaatgcga taaatataga tactaaacta caaggtaaga
     9661 aaataaatct aactgaaaat gatattgatg atattataaa tgtatctaca ttgatttcca
     9721 aactaacagt ttttaaatct tctaaagtat tagaaaacta taaaaataaa tttttagaaa
     9781 tttatggtga aaacgaagat gtacaattgt tgaaattatt aaatagcagc actggtttag
     9841 gcatacctaa agaatacaat ttatctagca atttaaatga tttgaaaaaa gcaaatgctc
     9901 tgtcaggatt attagaaaat tggaaaactg aggctttaat aaaaaatcaa gactctattg
     9961 tattaaataa tcgaagatta aaagagttaa aaccttattt attaaaagac aatataaaca
    10021 cgtcttttga tgtttttttt attaaattta ataaaactag cagcaaacta tatttaaaaa
    10081 caaactcggg ttcattacaa tctatgcaaa catatggaag atttatgtat atgtttaata
    10141 aacaattaaa gtatgaagtc aatgaatttt gtaaattcta tgttcctatg atgtctcata
    10201 aagaaattat atataatcac cctaatccta aattacaaaa tgtgatgtcc tcaacattta
    10261 gttcagatgc tatcgacttt ttgggagttg aaggaaattt aaatatagaa aatttatatg
    10321 tttgtcttgg tgaggatttt aagttttaca taaaagataa acaaacaggt aatataattt
    10381 ttcctgattt taaagatatg cataatacta atctatcacc tgttgtcatt agatttttat
    10441 ctgatatctc tctacaatat agtactggtg gttatttctt gaattattct gctacagagc
    10501 atgcttattc cccaaggata gaatataaaa acgtagttct ttctcctcgt aaatggcata
    10561 tgaattttcc aaaaaagttg aattttgatg cctttctaaa cgaattgaaa aaatttaaag
    10621 aactttataa tttagatata gttttttata taattaacga tgatcataaa ttatatattg
    10681 atacaagatt tgatatctct ttacaaattt tatatgacca gtataaaaaa caagaaattt
    10741 tagaagtaga agaagtagaa gaagaactat cttttaataa agatataggt attaacgaac
    10801 ttgtattctc agtttctaat aatgaaaatt caatttcaga aaaaaatata gctatgattg
    10861 ataaagatat aaggaattac aaagaggtta tattgcccgg taataattgg atatgtctaa
    10921 acttatatta tgatgaatac aattttaaag agtttatgaa taaaggaata tggaacgatt
    10981 tatttaaaga tataggaaaa aatcatgata ttgatactgt ttttttcatt aggtattttg
    11041 attcagatcc tcacataaga ttaagattta gaataaaaga aaacattaat aaaaacagaa
    11101 atgatattct aaataaacta aacaaattca aaaatgagaa ttttttaaaa actttctcta
    11161 tagttccgta ttatcgagaa agctatcgtt acggaggatt aaactgtatt catctagccg
    11221 aaaaatgctt tcaaatagat tctaaaattg tagctagata ctatggggag ctagatgata
    11281 aatcagacaa aatagatttt gctatagata atattataga aattttaaat ttattcacag
    11341 gtaagtgtat tgaagagaat ataaaaattt taagtgtttt tgggaaaaat aaagaaaata
    11401 aagacttata tagagagaaa agaaatcgaa tctttaaatc tatttctaat aatgataaat
    11461 atatatataa ttatggaatt agtgatttta gaaaaaaagt atatttaaag tatataaatg
    11521 aactaaaaaa ggaaaataaa ttaaataatc atgatataat acttagtatc attcacatgt
    11581 tttgcaatag attgttcgga attgatagag aaattgaaag taaagtatta gaaattatat
    11641 atagaagttt aattgattat aaaaaaataa actgatttat aggagtataa tgatggaaaa
    11701 tagtatccaa aaatccttat catacctttc agatataaag ttagttaaca aaaacattga
    11761 agataatata ggatcggacg ttatatttaa aattagttta gcgtatggat atcctggcat
    11821 agctttactt tttaacgaag catataaact cactaaaaac attgattatt atgaatattg
    11881 ccataattat cttagtgaaa ctttaaatct aattaaacat aacccaatgt attctacttc
    11941 attatttact ggtgttatgg gttacttatt cacactagca tcatgctcta atgatggcga
    12001 aaattataga aatataagtt atcaaatttt agatgaatat aatgcttttt tcaatgtatc
    12061 tttaaatgaa gttaacattg ctcttttaaa caaagatttt tcgaaagata gttttgatat
    12121 tataaatgga gctgctggta ctttgctaag tttactacat atatatgata tttatggtaa
    12181 tcttcattac aacttagaaa aatggataca aagtcttaca aacttgttag aaaaacttat
    12241 aataaaatcc ataagtaata attatccaga acatgaaaat gttttaacag acttaggagt
    12301 gtctcatgga ataactggta ttataaatat tgttaatgct gcatttaaaa gggattttgg
    12361 tagctataca ttacataata cattattacg tgctaaaaac tttttactat ccctaataga
    12421 aaaatatgaa agttcttata taatacctaa tttcagacat ttttctaaag aaaatttaaa
    12481 tcacagagat gcttggtgtt atggtactcc tggagttgct atggtactgt ataatttagg
    12541 aattaattta aaagacaaag aaattataaa tactagtaaa aaattggctt ctgaaacttt
    12601 gaaaagaaat aaatctacta gacttctaat ttcaccaaca ctatgtcacg gttatagcgg
    12661 aatagctatt attaataaat acttaggtaa tttttatgta gaagattatt attataatca
    12721 aattgaaaat tctaaaaata ctaatttaag attaatgttt agaaacatag attataacgg
    12781 agaaaatttt gtttatgatg acaatttagg tctattagag ggtagttctg gtgtactatt
    12841 aaccttactt tcaaagaagg gttttaattc aacttggtat aaattgtttt gttttgcgta
    12901 aacaatttta cattaaacat aattgaaagg tgatgtttta tgattttttt aacaaaaatt
    12961 ttagacacct tagcatatat atgtgttggt ttgatattct taaaatattt aatattaaca
    13021 gtaaatagtt gttttgattg gcatttaaga tggtattttt tagaaaatat accatatatg
    13081 gctattatat tatttgtagc tacatttatt tttgcggtac cttctgagat gattaaagat
    13141 aagttaaaag ataaataata cagattttaa aagaggtgct atgtgatttt acaactaaaa
    13201 agcgtgaact ggcctgaatt tatatttttt cctatagttt ttttttcttc tttccttgtg
    13261 aatattatac tttctttgat caacaatttt tttaatatca attttttttc agtattaggg
    13321 actaaaaatg tagtgataat gtctgatttt tcaataaatt taataataat ctttttattt
    13381 ttaattttag aaaaaaaaac aaaaaaaaat atagtggaat ctttagaata tattaagaaa
    13441 aacatttttc tttttatgtt tctttttact ttaccaaaat tagtaattat tttgattaac
    13501 tatttattta acttaagtaa tgtgaatttc aatttatcta agactgagaa tcaaatgcta
    13561 ataatagatt ggataaaaga acctaattac ttctttatga aaataataat ttttttatca
    13621 ataattctag tcggacctat atcagaagaa atactgtatc gtcatcttat tatcggagaa
    13681 ttaggtaaaa tcttttctta taaactcatg gcttttattt caattattct attttcttta
    13741 atacatgttt cagacgcaaa atcatctcta gaaatattaa cgtacttaat tttatcctta
    13801 tctttagtct taatatactt gatatcaaaa agaaatattt ttgtttctat aacattacat
    13861 tgttttatta atataacttc ttacgcgtac atatattatt tttattaata tttctatttc
    13921 aggaaggagc agttttttga caaaaatatt agattttcta tcaactgcat gctttgtatt
    13981 actgtcatct aaatatgcta tatcatcaat aaatatgata tttgacttaa aattacgttg
    14041 gtacatctta gaagatatac ctcatttatc tataatatta ctaattttaa ctttagtttt
    14101 cttcgtttct tcagaaatga taaaagataa taaagaaaaa aataaaaaat aaaaaatcat
    14161 tttatagaca acctctataa tatatagagg ttgtctggtt ctgttgcaaa gttagaaaaa
    14221 gtatagctaa tcatcatttt atcatgtcaa ggttcgttta actggctaac atatgactga
    14281 tttcatggca tggagaaaaa ccgtaaatct gaagagtgga ctgaccccaa ttagtgggaa
    14341 ttatataaaa cactttcgtt gaattcatat taaaatgaat catgcggagg tgttttttct
    14401 atgaaaagag ttgcttattc attttgaatt acatttctaa taaattcata atctccaata
    14461 tatcatctaa ataaactata cattcttctt cttaaaagca actacgacta atacaagaaa
    14521 gataacaata tagataatat ttccaatgaa taactcatgt aattctaact ttgttatttt
    14581 tttgaatgta tcgttgtcag taatttgcgc cataatattc atcatattca gaggattcca
    14641 ttttaaccat tcccatttat ctataactac tgttaataaa ccagagataa ttgagacagc
    14701 aaagtaaaac acaataccta cagcgattga tacgccagga cttttcataa tgcatgagat
    14761 gagtaatgtt aagctcataa ctaaccacat tccaacaaat gtaccaaggg ctgttaataa
    14821 catagagttt aatagtgata tatgattttt agagtgttca gttaaactcg tctctccatt
    14881 aaagattaaa tgtagtaata atgaaataat aataaagacg agaaaaataa taagtgaata
    14941 aataagtaat gtaataaatt tactgactat aatacttgta cgtgaataat ttctgtataa
    15001 taaattttta atcgttccat aatgaaattc cattgaaata attgtacttg cttgaataat
    15061 cattaaaaat acaacccaag aaaatgaaga aaaaccacct ttaaaacttt cctttaagtt
    15121 aaaaacatca gaataatgat ttcccaaaat ggctaaagat aacataatta ttattataat
    15181 aataggtaat atatatgttg atttcttctt aaataactta aaacattctt gtttaactaa
    15241 tgtacccatc ttacatttca ccttctttat caataatatt taataagaca ttttccaaat
    15301 cgtcttcatt ctcttgattt aattcttctt tagttgtttc tttaataatg gaaccatcat
    15361 taataattaa aatagaatca gttattttta ctaattcact taaaatgtga cttgatatta
    15421 aaaacgttgt tccttgctta gcagtttcta aaataagttc tcttacatct cttactgctt
    15481 taggatc
//
