Genomic loci for this biosynthetic pathway

Cluster Type From To
The following clusters are from record BGC0000509.1:
Cluster 1RiPP115487

BGC0000509, epilancin 15X biosynthetic gene cluster from Staphylococcus epidermidis. Locus 1. Full MIBiG entry.

Chemical compounds

Compound: epilancin 15X
Molecular formula: C145H233N41O33S3
Exact molecular mass: 3172.7 Da (M+)
Molecular activity: Antibacterial
Molecular target: unknown, pore formation

Class-specific details

Biosynthetic class(es):
RiPP
RiPP subclass:
Lantipeptide (cyclic)
Peptides in this cluster:
AFN69432.1
RiPP core peptide sequence(s): SASIVKTTIK ASKKLCRGFT LTCGCHFTGK K
Leader peptide length: 25
Cleavage recognition site sequence(s): SDLNPQA
Recognition motif in leader peptide for modification: FDLN
Peptidase(s) involved in precursor cleavage: afn69433.1
Crosslinks in final product:
12=16 AA (Thioether)
20=23 AA (Thioether)
22=25 AA (Thioether)

Gene cluster description

epilancin 15X (BGC0000509). Gene Cluster 1. Biosynthetic class = RiPP. GenBank JQ979180, positions 4943-14152. Click on genes for more information.

Legend:

biosynthetic genes
transport-related genes
regulatory genes
other genes

Detailed annotation

elxA leader / core peptide, putative Class I
MKKELFDLNLNKDIEAQKSDLNPQ - DhaADhaIVKDhbDhbIKADhaKKLCRGFDhbLDhbCGCHFDhbGKK

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

General MIBiG information on this cluster

Complete gene cluster sequence?complete
Evidence for cluster-compound connection:Enzymatic assays, Heterologous expression, Sequence-based prediction
MIxS-compliance:Unknown
Contact for this cluster:Wilfred A. Van Der Donk (The University of Illinois at Urbana-Champaign)

Literature references

1. Ortega MA et al. (2014) Substrate specificity of the lanthipeptide peptidase ElxP and the oxidoreductase ElxO. ACS Chem Biol 9(8):1718-25. doi: 10.1021/cb5002526. Epub 2014 Jun 6.
2. Velasquez JE et al. (2011) Biosynthesis of the antimicrobial peptide epilancin 15X and its N-terminal lactate. Chem Biol 18(7):857-67. doi: 10.1016/j.chembiol.2011.05.007.
3. Knerr PJ, van der Donk WA. (2012) Chemical synthesis and biological activity of analogues of the lantibiotic epilancin 15X. J Am Chem Soc 134(18):7648-51. doi: 10.1021/ja302435y. Epub 2012 Apr