LOCUS       BGC0000511             14150 bp    DNA     linear   BCT 26-JUL-2016
DEFINITION  Bacillus subtilis subtilin-like gene cluster, complete sequence.
ACCESSION   BGC0000511
VERSION     BGC0000511
KEYWORDS    .
SOURCE      Bacillus subtilis
  ORGANISM  Bacillus subtilis
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:43:52
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 14150
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             <1..225
                     /codon_start=1
                     /gene="proZ_opuBD"
                     /product="ProZ/OpuBD"
                     /protein_id="AAL15578.1"
                     /transl_table=11
                     /translation="VIAIGITAIGTFVGAGGLGDMIVRGSNATNGTAIILAGAIPTALM
                     AVLADLMMVWIERMLNQVKQKSEKKLIGV"
     gene            <1..269
                     /gene="proZ/opuBD"
     source          1..14150
                     /db_xref="taxon:1423"
                     /mol_type="genomic DNA"
                     /note="eriBSA1348"
                     /organism="Bacillus subtilis"
                     /strain="A13"
     protocluster    1..14150
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[438:7248]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      439..7248
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..14150
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..14150
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000511"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..14150
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     regulatory      233..269
                     /gene="proZ/opuBD"
                     /note="TerB"
                     /regulatory_class="terminator"
     regulatory      294..300
                     /gene="eriB"
                     /regulatory_class="minus_35_signal"
     gene            294..3531
                     /gene="eriB"
     regulatory      319..325
                     /gene="eriB"
                     /regulatory_class="minus_10_signal"
     regulatory      372..385
                     /function="catabolite repression"
                     /gene="eriB"
                     /note="Cre1 box"
                     /regulatory_class="other"
     CDS             439..3531
                     /codon_start=1
                     /gene="eriB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     849.9; E-value: 4e-257)"
                     /gene_kind="biosynthetic"
                     /product="putative SpaB"
                     /protein_id="AAL15564.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.3e-126,
                     bitscore: 413.3, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 1.9e-33, bitscore:
                     106.3, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 2.1e-14,
                     bitscore: 44.2, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSLYTLENNYMIRVPLVHHVLKNENFQDTDQLLHDFGSDSLFRE
                     QILVSSKTLYDTMNTFIQSPDKLKGKKKRNFRQAILKYAIRRATRTTPFGLFSAVGIGS
                     FSNENQLSFNQHSFYKKARVDFEWLYQLIKKLESEYTELLSFTINPACYIKGDRAYLLY
                     STDGKSEEVSVRATSVFYLINELCGECVAYQEIIRSLTENYENTPISKINQYVEDLIDK
                     EFLISNLRPPMTVSDQFQYLIAQAESSQIPNEFLRACRKIQYQIDEYNRITIGKGEYKY
                     LNLIETMNELIKTSSPLQVDTGLDDFSIQLDNETSLAISELASVFTYMAVPFAKRLDHL
                     EKYKNVFLERYGYEREIPLLEMLCSSAGIGAPATYTNPSNEFFEETSFGEQFSPEIKQF
                     FMRKYFESVRKKTPIQLNDETFHRICNSEIADEEIPVSFELNFFLKLRNGRVLLYLGPN
                     VGSTRAGKTFGRFTHLSDSISTVIKNIHNKEKDIIDSKVKICELSIVPNQVRSGNVTRN
                     ENYREKEMTLFTNSVLHHSDSVKAEDVLIGINKNQIFYARHKSTGEILSFESNHMFNPL
                     LMPNALRFLLEISRDGKREWNNFPWLSIYSDFKYIPEIKYKEIILSCEQWLVHKSDLSM
                     HSNSSIEEAKTAFFEFHCTYNLPQTFYIVNADNRLLIDIEDDRTLDVFFWELKKTDHNQ
                     PLQLVAAEHDADALKDRNQNVYSGEIVVPLVRNKPEKPLDLPIFNSIEENRFERIKMPF
                     EDWLFIKLYCKQTREEELIAFEIADFYNETSRKFPVRHFFMRYRDPKPHIRLRFNGKVE
                     VLYSLFPQLLNWLKSLREKGLVTEFVITQYEREIERYGGPELMDAAEELFCEDSKVVET
                     MIRMHRMKDIALSNEIAGMVSVIQFLEQFELTFEEQLTFLEKNCLQNEYRTEFKKDREK
                     YIEICNSDRDWDNLRKTSDGRILYEILKVRKIAAANYACLIKKEFNNKEAVFSRIGSVI
                     HLHCNRLFGTDREMENKILTLCRHSLYAQRYHKKNGSLAWK"
     regulatory      846..859
                     /function="catabolite repression"
                     /gene="eriB"
                     /note="Cre2 box"
                     /regulatory_class="other"
     gene            3522..5366
                     /gene="eriT"
     CDS             3522..5366
                     /codon_start=1
                     /gene="eriT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 171.2; E-value:
                     4.9e-52)"
                     /gene_kind="transport"
                     /product="putative SpaT"
                     /protein_id="AAL15565.1"
                     /transl_table=11
                     /translation="MEVKEQLKLKELLFIMKQMPKTFKLIFTLERALFIKLIVFSIITG
                     ILPIVSLYISQELINSLVTIQKDVSIVISIFLTYLGVSFFSELISQISEYYNGKFQLNI
                     GYKLNYKVMKKSSNLALKDFENPEIYDKLERVTKEISYKPYQIIQAIITMTTSCVTLLS
                     SIAFLMSWNPKVSLLLLVIPVISLFYFLKIGQQEFFIHWKRAGQERKSWYISYILTHDF
                     SFKELKLYNLKDYLLNKYWDIKKTFIGQDTKILRKKTYLNLIYEIAVQLVGAVIIFIAI
                     MSAFAGKIMVGNVMSYIRSVSLVQNHSQSIMTSIYSIYNSNLYMNQLYEFLELKEEKNE
                     GHKKQIVEPIDSVVFQNVSFIYPNQGEQTLKHINVSLHKGERVAIVGPNGSGKSTFIKL
                     LTGLYEVQQGDILINGINIKELDMDSYMNQIAALFQDFMKYEMTLKENIGFGQIDKLHQ
                     TNKMHEVLDIVKVDFLKSHSSYQFDTQLGLWFDEGRQLSGGQWQKIALARAYFREASMY
                     ILDEPSAALDPIAEKEIFDTFFSLSKDKIGIFISHRLVAAKLADRIIVMDNGEIVGIGK
                     HEELLKTCPLYKKMDESENYMNPLEEERSKWTEVQYQG"
     gene            5339..6664
                     /gene="eriC"
     CDS             5339..6664
                     /codon_start=1
                     /gene="eriC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     400.7; E-value: 1.1e-121)"
                     /gene_kind="biosynthetic"
                     /product="putative SpaT"
                     /protein_id="AAL15566.1"
                     /sec_met_domain="LANC_like (E-value: 1.1e-85, bitscore:
                     277.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDRSTVSGIEIETVKKIARQMSNFDKVLEIVNQKDNFHSIGGVPL
                     IPWKSTALSHGIPGICMLYGELNAHFPAEGWDELGHQYLSMLVDEIKEKGLQTPSMFSG
                     AAGIGLAAVCLSQDFAYYNGFIARINEYLAEVVPLLLNEYKQRQLYMTDYDVIEGVSGI
                     ASYLLLFQEDKRMKDLLIQILKYLVRLTEDINVKGEKVPGWHIPSLHQFTDIEKKSYPY
                     GNFNIGLAHGIPGPICILSSALMEGIKVNGQEHAVEKMVEFLLEFSQNDKDHLYWKGII
                     SFEEYQYGLQPSTVNFSRDAWCYGRPGVCLALVKAGKALRNNQYINLGVQNLKYTISDI
                     QGIFSPTICHGFSGIAQILLGVNQLTGQEHFKDELEKIKRKIVSFYDKNYIFGFHNYES
                     LEGNKVVPLQYVGLLDGVIGVGLGILNMELGSKTEWTKALLI"
     gene            6728..6898
                     /gene="eriSa"
     CDS             6728..6898
                     /codon_start=1
                     /gene="eriSa"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_a"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /product="lantibiotic ericin Sa"
                     /protein_id="AAL15567.1"
                     /sec_met_domain="TIGR03731 (E-value: 2.8e-23, bitscore:
                     71.8, seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_a (E-value: 3.8e-08, bitscore:
                     22.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNMSKFDDFDLDVVKVSKQDSKITPQVLSKSLCTPGCITGPLQT
                     CYLCFPTFAKC"
     CDS_motif       6728..6808
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MTNMSKFDDFDLDVVKVSKQDSKITPQ"
                     /locus_tag="eriSa_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       6809..6898
                     /prepeptide="core"
                     /locus_tag="eriSa_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="27.80"
                     /molecular_weight="2987.6"
                     /monoisotopic_mass="2985.5"
                     /core_sequence="VLSKSLCTPGCITGPLQTCYLCFPTFAKC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MTNMSKFDDFDLDVVKVSKQDSKITPQ"
                     /leader_location="[6727:6808](+)"
                     /alternative_weights="3005.6"
     CDS             6892..7248
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_kind="biosynthetic"
                     /note="orf1"
                     /product="unknown protein"
                     /protein_id="AAL15568.1"
                     /sec_met_domain="LANC_like (E-value: 1.1e-19, bitscore:
                     60.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLKIVNQKDNFHSIGRVPLILWKSTALSHGIPGICMLYGELNAHF
                     PAEGWDELGHQYLSMLVDEIKEKGLQTPSMFSGAAGIGLAAVCLSKDFAYYNGFIARIN
                     EYLAEVVSYGQKHY"
     CDS             7326..7496
                     /codon_start=1
                     /gene="eriSb"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_a"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="similar to ericin Sa"
                     /product="unknown"
                     /protein_id="AAL15569.1"
                     /sec_met_domain="TIGR03731 (E-value: 4.2e-24, bitscore:
                     74.5, seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_a (E-value: 5.2e-08, bitscore:
                     22.3, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKFDDFDLDVVKVSKQDSKITPQWKSESVCTPGCVTGVLQTCFL
                     QTITCNCHISK"
     CDS_motif       7326..7397
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MSKFDDFDLDVVKVSKQDSKITPQ"
                     /locus_tag="eriSb_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       7398..7496
                     /prepeptide="core"
                     /locus_tag="eriSb_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="27.80"
                     /molecular_weight="3343.9"
                     /monoisotopic_mass="3341.6"
                     /core_sequence="WKSESVCTPGCVTGVLQTCFLQTITCNCHISK"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MSKFDDFDLDVVKVSKQDSKITPQ"
                     /leader_location="[7325:7397](+)"
                     /alternative_weights="3361.9"
                     /alternative_weights="3379.9"
                     /alternative_weights="3398.0"
     gene            7326..7639
                     /gene="eriSb"
     regulatory      7587..7639
                     /gene="eriSb"
                     /note="TerS"
                     /regulatory_class="terminator"
     gene            8048..8554
                     /gene="eriI"
     CDS             8048..8554
                     /codon_start=1
                     /gene="eriI"
                     /product="putative SpaI"
                     /protein_id="AAL15570.1"
                     /transl_table=11
                     /translation="MFLKRCVIVFGCFIVLFMLSACQSLTKFKDKVYEETKKTMHFTDD
                     NENETSETTESLIDQGKHDQVVYDGKIYQLQDKVDKDIKGKVIGAIGKTFFVDGDGKRW
                     SEEELKEPYIYMDPDKIRDKNPFRYGKVYSTKEHPDTKDEIIVECNSEYYKAIIINNEK
                     DKGRE"
     gene            8529..9272
                     /gene="eriF"
     CDS             8529..9272
                     /codon_start=1
                     /gene="eriF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 180.9; E-value:
                     5.3e-55)"
                     /gene_kind="transport"
                     /product="putative SpaF"
                     /protein_id="AAL15571.1"
                     /transl_table=11
                     /translation="MKRIKGESDMSSVLLETKGLTKKFGKETAVADVSLTVQKNSIYGL
                     LGPNGAGKSTTLKILTGILRKTSGEIFFDGHKWKRSDLKNIGSLIESPPLYDNLTAFEN
                     VKVHATLLGLSKERINSVLETVDMLHAGKKRAGQFSMGMRQRLGIAIALLNHPKLLILD
                     EPTNGLDPIGIQELRDLIRSFPLEGITVILSSHILSEVEQIADHIGIISDGHLGYQGEI
                     NKADNLEQLFMEVVKSSREKGGAKK"
     gene            9269..10024
                     /gene="eriE"
     CDS             9269..10024
                     /codon_start=1
                     /gene="eriE"
                     /product="putative SpaE"
                     /protein_id="AAL15572.1"
                     /transl_table=11
                     /translation="MKNYIKAENLKFKRTFSRRMIIFVPLLNIVFSFLMNIQFFVSGTF
                     NWWSIIFMPVMISLFCSLSHQKEKRASNYNGTYSLPVDLEKLWYAKIIIIMIYSLISQI
                     VFLIFMFLMGFVIADLSNVSLSIIAASILLWITSLWQIPLCLYVARKWGFTIAVMFNLI
                     GALVLGIMPASRAFWWFIPWSWPIRMMCPAIGIHPNGLLLEKNDPLLSWAVVPSAVFIS
                     IVLLLILAFLTSRSFAPAKDYRTNKQEAM"
     gene            10185..10796
                     /gene="eriG"
     CDS             10185..10796
                     /codon_start=1
                     /gene="eriG"
                     /product="putative SpaG"
                     /protein_id="AAL15575.1"
                     /transl_table=11
                     /translation="MEAIAVAMPLLIGVLCGMTASLEEQAGKFKVLLGSTSPKIIAYVS
                     KTLLLILMNICSISISIISYLLGLKFILKVPNLSYTIFISGGAWLVAGSIALYFIYFFI
                     SLMFGTGASVLIGGAGLLMTSLMNTGLGDIIWKYNPWAWGVRLSGLNGMLQFKEINEKL
                     KPFFIQEINIGALIVIFSIICLFTVSLIWFSRWEGRQTFE"
     gene            10814..11476
                     /gene="eriR"
     CDS             10814..11476
                     /codon_start=1
                     /gene="eriR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 168.9; E-value: 2.1e-51)"
                     /gene_kind="regulatory"
                     /product="putative response regulator"
                     /protein_id="AAL15573.1"
                     /transl_table=11
                     /translation="MANILAVDDEKDILVLIKNILRRDQHQVDTLISVQGQPLDIFQGY
                     DLILLDVMMPNIDGFALCKQIRPLVDCPILFLTAKTEEEAIVNGLITGGDDYITKPFGI
                     MELSARVNAHLRREHREKHQSKRVISGFLFHFDSKEVFINNHKLNLTKNEYKICEFLAQ
                     NKGRIFSREQIYEEIYGIEGNALYSTITEFIRTIRKKCKEHNADPIKTIWGVGYRWE"
     gene            11449..12840
                     /gene="eriK"
     CDS             11449..12840
                     /codon_start=1
                     /gene="eriK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 140.5; E-value: 1.6e-42)"
                     /gene_kind="regulatory"
                     /product="putative histidine kinase"
                     /protein_id="AAL15574.1"
                     /transl_table=11
                     /translation="MGRRLQMGVGFKGKKTLLKELIKYMATLCISLVVVAVLYLFINIL
                     AMNTGFSYPANYNEREAEKLVPKLATVDKVTPDMIPDTMSYAILDKKSKKETAGTVKEE
                     DLHLVKKKIEKKPYVNYKQKGYLVIERKNEYCVLQYSLRADFSSPLLRKYLPNYELTSM
                     CILVILFITVIFIVTTYFANGLKKHFETLNIITRSIKEQDLQFKPEFTNIKEFDCVIDS
                     LLDMKDALQNSLEAQWRLEKNKKEQIGALAHDIKIPMTIIKGNAELLSLSIQNEEQAEY
                     TKYILGAGNRIEQYIHQLIHLSKTEESLTINLEKASIDELTNALQEDISAYKGNKEVNI
                     LFKKENLMHKVKVDCQLLHRALLNILTNAVDHTPEGGGVSVQAECDYANFLFVVTDTGN
                     GFSQDGLKKATDLFYMDDKSRHSNGHYGMGLTFAKNVVNLHNGELKIGNSQYGGGQVRV
                     SIPIR"
     CDS             complement(12924..13031)
                     /codon_start=1
                     /gene="pseudo12923_13031"
                     /note="orfA; similar to YxvaQ; unknown"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRNPFPKSKKQFRLASDHPIQWFKCSRISKNFNRL"
     CDS             complement(13389..13709)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs)
                     SMCOG1167:transcriptional regulator (Score: 101.8; E-value:
                     2e-31)"
                     /gene_kind="regulatory"
                     /note="orfB; similar to YvaP"
                     /product="unknown"
                     /protein_id="AAL15576.1"
                     /transl_table=11
                     /translation="MNQSEMCPRFEKAVDILSKRWVALIVFQLLNGQQRFSEIEAALPN
                     LSGRVLSERLKELETEGVIKRDVIPETPVRIEYSLTEKGCALAPILEEISKWATEWIDP
                     FD"
     CDS             complement(13791..14033)
                     /codon_start=1
                     /note="orfC; similar to YvaO"
                     /product="unknown"
                     /protein_id="AAL15577.1"
                     /transl_table=11
                     /translation="MARAGHIKAFQEEIRETSESYQSICAIYQTAVDRGAEHLPLFDSK
                     KWQHFTKEDIESVSKYFDFIAAEAKKRSPDSSSSF"
ORIGIN
        1 gtcattgcca tcggcatcac ggcgatcggc actttcgtcg gggcgggcgg cctcggcgat
       61 atgatcgtca gagggtcgaa cgcgacaaac ggaaccgcca ttattttggc gggcgccatc
      121 ccgaccgcgc tgatggcggt attagctgat ttaatgatgg tctggattga aagaatgtta
      181 aatcaagtga aacaaaaaag tgaaaaaaag cttataggtg tttagaataa aaaaaggatt
      241 cttttcttag gggaaaagaa tcctttttat atggggttta tcgggctata tcttgatact
      301 ttcttgattt tccaaatgta tattataaat agtacattta taataatttt cataaaatag
      361 aattaaatgg aggttatcgc gggaaaatgt tgaatagttt ttatatacag tgaatacata
      421 ggggggctat agactaatat gaaatcctta tatacacttg aaaataatta tatgattcgg
      481 gttcctttag tacatcatgt cttgaaaaat gagaactttc aggataccga tcagttgttg
      541 catgactttg gcagtgattc actctttcgg gaacaaatac tggtatctag taagacatta
      601 tatgacacaa tgaatacttt cattcagtca ccggataaat taaaagggaa aaaaaagcgg
      661 aacttccggc aggcaatttt gaagtacgca attagacgag caacaagaac aacacccttt
      721 ggcctttttt ctgcagtggg tattgggtct ttttctaatg agaatcaatt atctttcaat
      781 cagcattcat tttacaaaaa agctcgggtt gattttgaat ggctctatca attaatcaaa
      841 aaactagaga gcgaatacac tgagttgctt tcttttacaa taaatcccgc ttgttacatc
      901 aaaggtgatc gggcttactt gttgtatagc acagatggaa aatctgaaga ggttagtgtt
      961 cgtgcaacat cggtttttta tctaataaat gaactctgtg gtgaatgtgt tgcttatcaa
     1021 gaaattattc gtagtttgac agagaactat gaaaataccc caataagcaa aattaatcaa
     1081 tatgtagaag accttattga caaagagttt ctgatatcaa atttacggcc gccaatgact
     1141 gtttcagatc aatttcagta tttaattgct caagcggaaa gcagccaaat tccaaatgag
     1201 tttcttcggg cttgcaggaa aattcaatat caaatagatg aatataaccg gatcactatc
     1261 ggaaagggag agtacaagta tttaaatctg attgagacaa tgaacgaact cataaagact
     1321 tcatctcctt tgcaagtgga cactggatta gatgacttct ccattcaact agataatgaa
     1381 acatctcttg cgataagtga attggctagc gtgtttactt atatggctgt ccccttcgca
     1441 aaaagattag accacttaga aaagtacaaa aatgtgtttt tggagcgtta tgggtatgaa
     1501 agagagattc ctcttttaga aatgctttgt tccagtgcgg gcattggtgc tcctgctact
     1561 tacacgaatc cttctaatga attcttcgaa gaaacatcat ttggggagca attttcgcct
     1621 gaaataaaac aattttttat gagaaaatac tttgaatcag ttagaaagaa aactcctatt
     1681 caactaaacg atgaaacatt ccatagaata tgcaactctg aaattgcaga tgaagaaatt
     1741 ccggtgtcgt tcgaactcaa tttctttctt aaattacgta atggcagagt cctgctttat
     1801 ttagggccca atgtcggatc tacgcgtgct gggaaaacat ttggaagatt tactcactta
     1861 tctgattcaa tcagtactgt cataaaaaat atacacaaca aagaaaagga tataatagac
     1921 agtaaagtaa aaatttgtga gttgagtatc gtacctaatc aggtcaggtc tggaaatgta
     1981 acaaggaatg aaaactatcg ggaaaaagaa atgactcttt ttacaaacag tgtcctgcat
     2041 cacagtgatt ctgttaaagc agaagatgtt ctaattggga tcaataaaaa ccaaatcttt
     2101 tatgccagac ataaaagtac tggtgaaatt ctttcttttg aatcaaatca tatgtttaat
     2161 cctttattaa tgccaaacgc cttaaggttt ttattagaaa tttcaagaga cggcaaaagg
     2221 gaatggaata atttcccgtg gttgagcatc tacagtgatt ttaagtatat tcctgaaatc
     2281 aaatataaag aaataatctt atcatgtgaa caatggttgg tacacaaaag tgatttaagc
     2341 atgcattcga attcatcgat agaagaggcg aaaactgctt tttttgaatt tcactgtact
     2401 tataaccttc cgcaaacatt ttatatcgtt aacgcagata atcgattatt aattgatata
     2461 gaggatgatc gtactttgga tgtttttttc tgggaactta aaaaaacgga tcacaaccag
     2521 ccattacaac ttgtagctgc tgagcatgat gcagatgctt taaaggatag aaaccaaaat
     2581 gtctattcgg gggaaatcgt cgttccgctt gtcagaaata aaccagaaaa accattggat
     2641 ttaccgatct ttaactcgat agaggaaaat aggttcgaaa gaattaaaat gccttttgaa
     2701 gattggctgt ttattaaact ttattgtaaa caaactagag aagaagagct cattgccttt
     2761 gaaatagctg atttttataa cgaaacttcc cgtaaatttc cagtaaggca tttctttatg
     2821 agatatcgag atccaaagcc acatataaga cttagattta atggaaaagt agaagtgctg
     2881 tacagcttat ttccacaatt attgaattgg ctgaaaagct taagagaaaa agggctggtt
     2941 acagagtttg ttatcaccca atatgagcgg gagatagaac gatatggcgg gccagaacta
     3001 atggatgcgg cagaagagct tttctgtgaa gatagtaaag ttgttgaaac gatgattaga
     3061 atgcatcgga tgaaggacat tgcgttaagc aatgaaatcg caggtatggt ttctgtcata
     3121 caatttttag aacagttcga gcttacgttt gaagagcaat taactttttt agagaaaaat
     3181 tgtctgcaga atgagtatcg tactgaattt aagaaggata gagaaaagta tattgaaata
     3241 tgcaactctg atagagattg ggataatctc agaaaaacaa gtgatggcag aatattatac
     3301 gaaatattga aagtaagaaa gatagctgca gctaattacg catgtttaat aaaaaaggaa
     3361 tttaataaca aagaagcagt attttcacgt ataggtagtg taatccacct gcattgcaat
     3421 cgtttattcg gaaccgatag agaaatggaa aataaaatac ttacactttg cagacattca
     3481 ttatatgcgc agcgatatca taagaagaat ggtagtttag catggaagta aaggaacaac
     3541 tgaagctaaa agagctgctg tttatcatga aacaaatgcc taagacattc aaattgattt
     3601 ttaccttaga gagggcactg tttataaagt tgattgtatt cagtattatt actggtattt
     3661 tgcctattgt gtcactatat atttcacaag aattgatcaa ttcccttgtg actattcaga
     3721 aagatgtttc aattgttatc agcattttct tgacatatct aggggtatcg tttttttcgg
     3781 aactcatttc gcagatttca gaatattata atggtaaatt tcaattaaat attggctata
     3841 aacttaacta taaagtcatg aaaaagagca gtaatctagc tcttaaggat ttcgaaaatc
     3901 cagaaatata tgacaagtta gagagggtaa caaaagagat tagttataag ccctatcaga
     3961 ttattcaagc catcatcaca atgaccacat catgtgtgac gctgctctca tccattgcgt
     4021 ttttgatgtc atggaaccct aaagtatccc tgctattact agttattcca gtgatttctc
     4081 tattttattt cttgaagatt ggacagcaag aattttttat acactggaaa cgggcaggac
     4141 aggaaaggaa atcatggtat atcagctata tactcacaca tgacttttct tttaaagaat
     4201 taaaattgta taaccttaag gattatttat taaacaagta ctgggatatc aaaaaaacat
     4261 ttataggaca agatacaaaa atattgagaa agaaaacgta tttaaacctc atatatgaga
     4321 ttgcagtgca attagttgga gctgtcatta tctttattgc catcatgtcg gctttcgcgg
     4381 gaaaaataat ggtcggtaat gtaatgagtt atatcagatc ggtttctctg gtgcaaaatc
     4441 actcacaatc tattatgaca agcatttatt ccatctataa cagcaatctc tatatgaatc
     4501 aattgtatga gtttctggaa ttaaaagaag agaaaaatga aggtcacaag aaacaaattg
     4561 ttgagcctat tgattctgtt gtttttcaaa atgtcagctt tatctatccc aatcaaggag
     4621 aacagacact gaaacatatt aatgtctcat tgcataaagg agaacgtgta gccattgttg
     4681 gaccaaacgg atcagggaaa agtacattta ttaagcttct taccggctta tatgaagttc
     4741 agcagggcga cattttaatt aatggaataa atataaaaga actggatatg gacagttata
     4801 tgaaccaaat tgcagcgcta tttcaagact ttatgaaata cgaaatgaca ttaaaagaaa
     4861 atataggatt cggacaaatt gataaactac atcaaacaaa caaaatgcat gaagttctcg
     4921 atattgtgaa ggtcgatttc ttaaaaagcc actcttccta tcaattcgat acacagctcg
     4981 gactatggtt tgatgaagga agacagctat ccggtggaca atggcaaaag attgctttgg
     5041 caagagcata ttttagagag gcttctatgt atattcttga cgaaccaagt gctgctttag
     5101 atcctattgc tgaaaaagaa atcttcgata cctttttcag tttgtctaag gataaaatag
     5161 gaatatttat ctcccatcga ttggttgcag ctaaattggc agaccgaatc attgtgatgg
     5221 ataatggaga gattgtcgga ataggcaaac atgaagagtt attaaaaaca tgtccgctgt
     5281 ataaaaagat ggatgagtcc gaaaactaca tgaatccact agaggaggaa cgaagtaaat
     5341 ggacagaagt acagtatcag ggatagaaat tgaaactgtt aaaaaaattg ctcgtcaaat
     5401 gtcaaatttc gataaagtgc tggaaattgt gaatcaaaaa gataattttc atagtattgg
     5461 tggagttccg cttattcctt ggaagtcgac tgctttaagc catggcatac caggcatttg
     5521 tatgctgtac ggtgaattga acgctcattt tcctgctgaa ggatgggacg agctgggaca
     5581 tcaatattta tctatgttag tagatgaaat aaaagaaaaa gggctgcaaa ctccgtcaat
     5641 gttttcggga gcagcaggta ttggactggc agctgtctgc ttatcgcaag attttgccta
     5701 ttataatggt tttatcgctc gtattaacga atatctggct gaagttgtac ctttattgct
     5761 aaatgaatat aaacagagac aactttatat gactgattac gatgttatag agggtgtcag
     5821 cggaattgcg agctacctgt tgttgtttca ggaagataag agaatgaagg atttgttaat
     5881 tcaaatttta aaatatctcg ttaggttaac agaagacatt aatgtgaaag gagaaaaagt
     5941 cccgggttgg catattccat ctctacatca gtttactgat attgaaaaaa aatcttatcc
     6001 ttacggtaat tttaatatcg gtttagcaca tggaatacct ggtcctattt gtattctatc
     6061 ctcagcgctt atggaaggaa ttaaagtaaa tggtcaagag catgctgttg aaaaaatggt
     6121 cgagtttctt ctagagtttt cacaaaatga taaagatcat ttgtattgga aaggaatcat
     6181 aagctttgaa gagtatcaat atggattaca gccaagtaca gtaaatttta gcagagatgc
     6241 ttggtgttat gggagacctg gtgtttgttt agcgcttgtt aaagctggaa aagcccttcg
     6301 aaataatcaa tatataaatc tcggagtaca gaatttaaag tatacaatct ctgatataca
     6361 aggaattttt tcacctacca tatgccatgg ttttagcgga attgctcaga ttttgcttgg
     6421 agtgaatcaa ctgaccgggc aggagcattt taaagatgaa cttgaaaaga ttaaacgaaa
     6481 gattgttagt ttctatgata aaaattatat ttttgggttc cataactatg aatcgttaga
     6541 agggaacaaa gtagttcctt tacagtacgt tggtctattg gatggagtaa taggtgtagg
     6601 actaggaatt ttaaatatgg aattaggatc aaaaacagaa tggacaaaag cattattaat
     6661 ttgaataaaa agtaaaaaat gataaaatat tgatattcgt ctgatagtat ataggtattg
     6721 aaaggaggtg actaatatgt caaagttcga tgacttcgat ttggatgttg taaaagtatc
     6781 taaacaagac tcaaaaatta ctcctcaagt gttaagtaaa tcactttgta caccagggtg
     6841 tataactggt cctttgcaaa cttgctacct ttgttttcca acatttgcta agtgctgaaa
     6901 attgtgaatc aaaaagataa ttttcatagt attggtagag ttccgcttat tctttggaag
     6961 tcgactgctt taagccatgg cataccaggc atttgtatgc tgtacggtga attgaatgct
     7021 cattttcctg ctgaaggatg ggacgagctg ggacatcaat atttatctat gttagtagat
     7081 gaaataaaag aaaaagggct gcaaactccg tcaatgtttt cgggagcagc aggtattgga
     7141 ctggcagctg tctgcttatc gaaagatttt gcctattata atggttttat cgctcgtatt
     7201 aacgaatatc tggctgaagt tgtatcttat ggacaaaagc attattaatt tgaataaaaa
     7261 gtaaaaaaag ataaaatctt gatatttgtc tgatagtata taggtattga aaggaggtga
     7321 ctaatatgtc aaagttcgat gacttcgatt tggatgttgt aaaagtatct aaacaagact
     7381 caaaaatcac tccgcaatgg aaaagtgaat cagtttgtac accagggtgt gtaactggtg
     7441 ttttgcaaac ttgcttcctt caaacaatta cttgtaactg ccatatctct aaataagtaa
     7501 aaaaccagga ttttgttctg actcttgcct tctcagtgtt atacataatt attttcccag
     7561 agttagaacg caaaaaaata tttttacaat taagtaacga gtgtgaaaga gctttaattt
     7621 cacactcgtt ttttatttga ctgttgataa atagacagga gtgaaaaaag tcattcgatg
     7681 actgtagtta tatccacagt ctttccgcca aatacaaaga cggttttact atgttggtat
     7741 tctcaagata acatatttta gacggatgaa aaaagagatt aaaccatcta aagtaagtaa
     7801 ggaatgttgc ctgatcacac tctttttata tggtcatata atcgagtcgc tgtaggccaa
     7861 atacttaaag tttccagctg gaaatgcatt ttgattaaat tttgataaaa gtattttaga
     7921 ataatcatca ttcgataaga aaagctctat tacaaagata tttgttcgta tggcaaagaa
     7981 tctttgatcc attttttata tttaacatct ttttggagga aatttcataa gagtgaggtg
     8041 atgtatagtg tttctaaaaa gatgtgtaat tgtttttggt tgctttattg ttttatttat
     8101 gttgtccgca tgccaatcat taacaaagtt taaagataaa gtttatgaag aaacaaaaaa
     8161 aacaatgcat tttactgatg acaatgagaa cgaaacatct gaaacaacgg agagtctgat
     8221 tgaccaggga aaacatgatc aggtggtgta tgacggcaag atttatcagc tgcaggataa
     8281 agttgataaa gatattaaag gaaaagttat aggggcaata ggcaaaactt tttttgttga
     8341 tggcgatgga aagagatggt ctgaagaaga actgaaagag ccttatatct atatggatcc
     8401 ggataaaata agggataaaa atccattccg ctatggaaag gtatacagta caaaggaaca
     8461 tcctgatact aaagacgaaa tcattgttga atgcaatagt gagtattata aagcgattat
     8521 aataaataat gaaaaggata aagggagaga gtgacatgag ttcagtccta ttagagacca
     8581 aaggtcttac aaaaaagttt ggaaaagaaa cagcagttgc ggatgtttca ttaaccgttc
     8641 aaaaaaatag catttatggt ttgttgggac caaacggagc tgggaaatca actacactca
     8701 aaattttaac gggaatttta cggaagactt ctggcgagat tttcttcgat ggacataaat
     8761 ggaaacgctc tgatttaaaa aatattggat cattaataga atctccacct ctttatgata
     8821 atttaactgc atttgaaaat gtgaaagtac atgctacgct gctcggtctt tcaaaggaac
     8881 ggataaacag cgtgctcgaa actgtcgata tgttacatgc tggaaagaaa agagcaggac
     8941 aattctcgat gggaatgagg cagcggcttg gaattgcaat tgctttatta aatcatccta
     9001 aattattaat cctcgatgaa ccaacaaacg ggctagatcc aattggtata caggagctta
     9061 gggaccttat tcgctcattt cctctagaag gaataactgt tattttatct agtcacattc
     9121 tttcagaagt cgagcaaata gcagatcata ttggtattat cagcgacggc catctgggat
     9181 accaaggaga aataaataaa gctgataatt tggagcaatt gtttatggaa gttgtcaaat
     9241 caagtcgtga aaaaggaggt gcaaaaaaat gaaaaattat ataaaagccg aaaacctgaa
     9301 gtttaaaaga acgttttcta gaagaatgat tatttttgta cctcttctta atatagtttt
     9361 ttctttttta atgaacattc aattttttgt atcaggtact tttaactggt ggtctattat
     9421 ctttatgccg gtgatgattt cattattttg ttctttatct catcaaaaag aaaaaagggc
     9481 ctctaattat aacggtacgt actcattgcc tgtagattta gaaaaattat ggtatgcaaa
     9541 gattataatt attatgattt attcactaat atctcagatt gtatttttaa tttttatgtt
     9601 tcttatgggt ttcgttatag ctgatttgtc caacgtttca ctttctatta ttgctgcgag
     9661 tatattactt tggataacaa gtctttggca aatccctctc tgtttatatg tagctagaaa
     9721 gtggggattc acaatagctg tcatgtttaa tctgataggg gctttagttt taggaattat
     9781 gccagcatct cgtgcttttt ggtggtttat tccttggagt tggccaattc gaatgatgtg
     9841 ccccgctatt ggtattcatc ctaacggctt gctgcttgaa aaaaatgatc ctttgcttag
     9901 ttgggcggtc gtaccttctg ctgtattcat ttctattgtc ttattgttga tccttgcatt
     9961 tctgaccagc cgttcgtttg ctcctgccaa agattacaga acaaataagc aggaggcgat
    10021 gtaagttgag actattattg aggtgcttaa aagctgattt ccagaaaaca aagaacacct
    10081 cctttatttg gttgcatatt cttatacctc tagtatgttc agttgtattt attctttatt
    10141 tttatgggag aaatcagagt caatttcaac agtataaaac ttttatggaa gctatcgcag
    10201 ttgctatgcc tcttctcatt ggggtgcttt gcggaatgac tgcatcatta gaagaacagg
    10261 caggcaaatt taaagtgtta ctgggaagca cttctccgaa aatcattgct tatgtgagta
    10321 aaacgctatt gctcatctta atgaatattt gctctatttc catatcaatt atcagttatt
    10381 tattggggtt aaagtttatt ttaaaagtgc cgaatttatc ttacactatt tttatatcgg
    10441 gtggcgcgtg gctcgttgcc ggcagtatag ctctttattt tatctatttt tttattagcc
    10501 tcatgttcgg aacgggggct tctgtcctta taggaggggc tggtctgtta atgacttcac
    10561 ttatgaatac aggtttaggt gatatcatat ggaaatataa tccttgggct tggggagtta
    10621 gattgtcagg gctaaacggc atgttacaat tcaaagaaat caatgagaag cttaaacctt
    10681 tttttatcca agaaataaat ataggtgcat taattgtgat atttagtata atatgtttat
    10741 tcactgtcag tcttatatgg tttagtagat gggaaggcag acaaactttt gaatagtata
    10801 gggggaccat ggagtggcga atatccttgc tgttgatgat gaaaaggaca ttcttgtcct
    10861 gataaagaat attttacgtc gtgatcagca tcaggttgac acattaatta gtgtacaagg
    10921 ccagccgctt gatatatttc aagggtacga tttaattttg cttgatgtta tgatgccgaa
    10981 tatagatggc tttgctcttt gtaaacagat ccggccgttg gttgattgtc cgattttatt
    11041 cctaacggct aagactgaag aggaagcaat agtgaacggc ctcattacag gcggagatga
    11101 ttatattaca aagccgtttg gtatcatgga gcttagtgcc agagttaatg cccatttaag
    11161 aagagagcat agagagaagc atcagtcgaa aagagtcatt tcaggttttt tgtttcattt
    11221 tgatagtaaa gaagttttta ttaataatca caaacttaac cttacaaaaa atgaatataa
    11281 aatctgtgaa ttcttagcac aaaataaagg aagaattttt tcaagagaac aaatttacga
    11341 ggagatatac ggaatagagg ggaatgcctt atattcaaca attactgaat ttattagaac
    11401 gattcgcaag aaatgtaaag agcacaatgc agatcccatt aaaacgatat ggggcgtcgg
    11461 ttacagatgg gagtaggttt caaaggaaaa aaaacgctcc ttaaggaact tataaagtat
    11521 atggcgacgc tatgtatcag tttggttgtg gtcgctgtgc tttatttatt tattaatata
    11581 cttgcaatga atactggatt ttcctatcca gctaattata atgaacgaga agctgaaaaa
    11641 cttgtgccaa aacttgcaac tgttgataaa gtgaccccgg atatgattcc ggatacaatg
    11701 tcgtatgcta ttcttgataa gaaatcaaaa aaagaaacgg ctgggaccgt taaagaagag
    11761 gatctgcatc tggtaaaaaa gaagatagaa aaaaagccat atgttaacta taaacaaaaa
    11821 gggtacttgg ttatagaaag aaaaaatgag tactgtgttt tgcaatattc attacgggca
    11881 gatttcagtt cgcctttatt aagaaaatac cttcctaact atgagctaac aagtatgtgt
    11941 atattagtca ttttgtttat aaccgttatt tttattgtga caacatactt tgccaatgga
    12001 ttaaaaaaac actttgagac actcaatata atcacacgat ctattaaaga acaggatttg
    12061 caattcaaac ctgaatttac gaatatcaag gagtttgatt gtgtaatcga ttctttactg
    12121 gatatgaaag atgctcttca aaattcactc gaggcacagt ggcgtttaga aaaaaataaa
    12181 aaagagcaaa ttggcgcttt ggcacatgat atcaaaattc cgatgaccat tattaaaggg
    12241 aatgctgaat tactcagctt atcaatacaa aatgaagaac aagctgaata tacaaaatat
    12301 atcctcggtg cagggaatag gattgaacaa tatattcatc agttgattca cctttcaaag
    12361 acggaggagt cactaacgat caatttggag aaagcttcaa ttgatgagtt aacgaatgct
    12421 ttacaggaag atatatcggc ctataaaggg aataaggaag ttaacatctt attcaaaaaa
    12481 gaaaacctaa tgcataaagt gaaggtagac tgtcaattgt tacatagggc tttattaaat
    12541 attcttacga acgcagttga tcatacgcca gagggtgggg gtgtctcggt tcaagcagaa
    12601 tgtgactatg caaactttct ctttgtggtg acagacacag ggaatggatt ttctcaggat
    12661 ggcttaaaga aagcgacaga tcttttttac atggatgata agagccgtca ttctaatggt
    12721 cattatggaa tggggcttac ttttgcaaag aatgtagtaa accttcataa cggagaactt
    12781 aagataggta atagccaata tggtggagga caagtgcgag taagcatacc tataagatag
    12841 aagtgataaa aacccatgct ttgattggaa ggcatgggtt atttattaaa taataaactg
    12901 atccatcttc tcattaagct ccttcacaat ctgttaaaat tcttcgatat tctagagcac
    12961 ttgaaccatt gaattggatg atcactcgct aatcgaaatt gtttctttga tttcgggaac
    13021 ggattcctca tatagagaag cgatattttg attgatagta atcgattggt ttgtaagttc
    13081 gtcacttcct taaaggtggt ttgtttgctc ttagttaagt gtcctcattt tgcttttgtc
    13141 tgtgtaaatc agaaaatcct tttctttaga agacaggtgc tcggtcaaat tgtgatatca
    13201 ttgatgccgt caattaaaac cattttgttc tgattgattt catctgtctt ttaaatgatg
    13261 cgctgcatat tataagtgcc ttcttcagat ggtgatctat gggtccttcg acttagatat
    13321 tgtattttca tatggaaaaa ataaaaaacg gctcttttct ctcttttgag acagaaccgt
    13381 tttatcaatt agtcaaaggg gtcaatccat tccgttgccc atttagatat ctcctctaaa
    13441 ataggggcta atgcgcagcc tttttccgtt aatgaatact caatgcggac aggcgtttcg
    13501 gggataacat cccgtttgat gacaccttct gtttcaagtt ctttcagacg ttctgacaag
    13561 actcttccgc ttaaattcgg aagcgccgct tcaatttcgc taaatcgctg ctgtccgttc
    13621 aataactgaa agacgattag agccacccag cgtttactta ggatgtcgac cgctttttcg
    13681 aatctggggc acatttctga ttggttcatg ctttttcacc tcgtcttctg ctattatacc
    13741 gcaaaatcgg agggaacgta cgtctggcgc ttgttaagtt gaggagagga tcaaaaagag
    13801 gatgaggaat ccggggagcg ttttttcgct tcagccgcaa taaaatcaaa gtatttgctg
    13861 acgctttcaa tatcttcttt tgtgaagtgc tgccactttt tgctgtcaaa cagcggcaga
    13921 tgctccgcgc ctctgtcaac ggcggtctga tagatggcgc agatggattg atagctttcg
    13981 gaggtttcac ggatttcctc ctgaaatgcc ttgatgtgtc cggcccgcgc cattaactcc
    14041 tcgtacggaa tttttaatgt gtccgccaat tttctgatgg tcgccggttt aggaatgccg
    14101 cggtgcccgt tttcaattct ggaaatggtc gccgagctga cgccggaata
//
