LOCUS       BGC0000514             12705 bp    DNA     linear   BCT 01-MAR-2007
DEFINITION  Staphylococcus gallinarum gallidermin gene cluster, complete
            sequence.
ACCESSION   BGC0000514
VERSION     BGC0000514
KEYWORDS    .
SOURCE      Staphylococcus gallinarum
  ORGANISM  Staphylococcus gallinarum
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:46:09
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     source          1..12705
                     /db_xref="taxon:1293"
                     /mol_type="genomic DNA"
                     /organism="Staphylococcus gallinarum"
                     /strain="Tu3928"
     misc_feature    1..12705
                     /note="gallidermin gene cluster"
     protocluster    1..12705
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5948:10119]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5949..10119
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..12705
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..12705
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000514"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12705
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(579..1271)
                     /gene="gdmG"
     CDS             complement(579..1271)
                     /codon_start=1
                     /gene="gdmG"
                     /note="putative ABC transporter subunit"
                     /product="GdmG"
                     /protein_id="ABC94897.1"
                     /transl_table=11
                     /translation="MKNELKVINLKFRTQILTYTPTLFTIFYITFINLYLGGNWNYTSI
                     SQYTSSFNALSSLIIAITTYQVIHFEESIGHFNHILGKSKRSLWVCATLSYIFINYLFC
                     LLISTVNFIVMTHNVKLTLFYVASSSFMNIIIILLIFIISLFTGSIFSMVSGVVITIFN
                     IYFGIEMLGDKSWYYIPITYSTRYTSMFINNSVPFFLTMSIYIISIIMMFICLLVLVKN
                     WSGRSIQD"
     gene            complement(1261..2025)
                     /gene="gdmE"
     CDS             complement(1261..2025)
                     /codon_start=1
                     /gene="gdmE"
                     /note="putative ABC transporter subunit"
                     /product="GdmE"
                     /protein_id="ABC94898.1"
                     /transl_table=11
                     /translation="MSLLKAEALKFKNTTAMYILFLSPLFFLAFALFTALFAQSSPNQH
                     DLSPFLSLLFNLWPYFVMPIIICISCSSMIGIEKRNSLFNYYVSNNWSINKMIKSKILV
                     MSIALLFHTIIMLFVAMIGNLLVGGNSVSLFLVLVTLFLVFFVSLPLIPLNFLFVKRFG
                     VFFSIFLNIALTIISIVFITITKFFWILPWSYIGRIPLITLSLNPNGTLMSKDSPYFND
                     LNSLSLTIIISIIYFSIFFMSNTKKSWNINEK"
     gene            complement(2027..2581)
                     /gene="gdmF"
     CDS             complement(2027..2581)
                     /codon_start=1
                     /gene="gdmF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 113.2; E-value:
                     2.6e-34)"
                     /gene_kind="transport"
                     /note="putative ABC transporter subunit"
                     /product="GdmF"
                     /protein_id="ABC94899.1"
                     /transl_table=11
                     /translation="MKIICGILPQTNGDVYLDGSPLTRQELTNIGSLIESPPTYNHLSA
                     QDNLKVVALNENIDFSEIPKVLDLVNLDVDPKKKVKNFSLGMKQRLGIAMALIKKPKLL
                     VLDEPSNGLDPYGIQELRDLLKNLTKANTSVIISSHILSEIQQLADHIGIIHNGKLEYQ
                     EKNKTDENLEEIFFNITKGGR"
     gene            2883..3875
                     /gene="gdmH"
     CDS             2883..3875
                     /codon_start=1
                     /gene="gdmH"
                     /note="putative ABC transporter subunit"
                     /product="GdmH"
                     /protein_id="ABC94900.1"
                     /transl_table=11
                     /translation="MYLLILLLLILIITTVILFKKHSRLNIYIVATAQLIFGLLFFYYI
                     SVIFNFSIYIIASMFIILSLSILVFIAFLIFNSKKLLEKEGSRLSNLLSLGLSLLLLWI
                     IVTSPVAFFINKSLASYIFVVYIFIVSLLIYFLSLFISCFILSLFYSRRKVNNNIRYIL
                     VLGSGLIEDKVPPLLQSRIDIGKNFFDSKKDADIKFLLSGGQGKDERISEAQAMKNYLI
                     SQGVNENKIICENKSQSTYENLQFSKKLIKKNTNILICSNNFHILRAVFIAKKLKYQHF
                     YSLGSKTKNYFFTNAFIRELIAILKMDWVFHLCIIISLIFWMVFTLFKL"
     gene            complement(3891..5165)
                     /gene="gdmT"
     CDS             complement(3891..5165)
                     /codon_start=1
                     /gene="gdmT"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 152.7; E-value:
                     2.4e-46)"
                     /gene_kind="transport"
                     /note="putitive ABC transporter subunit"
                     /product="GdmT"
                     /protein_id="ABC94901.1"
                     /transl_table=11
                     /translation="MNITRIFSEELSTFLTNLFSVIVSLTFLFVINKTLTLYLICTLPI
                     LVIVMLPIGKVMKRVSSKSQEATANLSSYYSNRLSIIKLIKTLSTYNVERKHNYSLLKK
                     LFDIELHKIKVLSFFEPIMNLLLFVNIFGILFFGYYLMENNVMKSGDMFAYILYLFQII
                     NPIVSITSYWTEVQRAIGASDRILKINKEQEEVFTVDTAYNNVAQKVKINDLNFTIENK
                     QIINSISLNFYKGYIYNIIGESGCGKSTLLNILAGLNTGYTGNIYLDDFDKEQFSKYEW
                     RSLFSYITQDLQILEDTIYNNLIYGIDETKNIEEVRAACEMTNSLFFIQNLKNGFNTQI
                     SPDSINLSIGQKQRLVLTRAFLQKKPIILLDEVTSNLDKESHKYIIKSIEELAQTSIVL
                     NVTHRHDKNDFSNSNVKLIDFRQFN"
     gene            5692..5850
                     /gene="gdmA"
     CDS             5692..5850
                     /codon_start=1
                     /gene="gdmA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gallidermin"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_ab"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_b"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /note="pregallidermin precursor peptide"
                     /product="GdmA"
                     /protein_id="ABC94902.1"
                     /sec_met_domain="Gallidermin (E-value: 9.7e-40, bitscore:
                     124.4, seeds: 3, tool: rule-based-clusters)"
                     /sec_met_domain="mature_ab (E-value: 1.7e-07, bitscore:
                     20.4, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="mature_b (E-value: 7.6e-16, bitscore:
                     47.2, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEAVKEKNELFDLDVKVNAKESNDSGAEPRIASKFLCTPGCAKTG
                     SFNSYCC"
     CDS_motif       5692..5781
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MEAVKEKNELFDLDVKVNAKESNDSGAEPR"
                     /locus_tag="gdmA_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       5782..5850
                     /prepeptide="core"
                     /locus_tag="gdmA_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="31.90"
                     /molecular_weight="2165.6"
                     /monoisotopic_mass="2164.0"
                     /core_sequence="IASKFLCTPGCAKTGSFNSYCC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEAVKEKNELFDLDVKVNAKESNDSGAEPR"
                     /leader_location="[5691:5781](+)"
                     /alternative_weights="2183.6"
     gene            5949..8876
                     /gene="gdmB"
     CDS             5949..8876
                     /codon_start=1
                     /gene="gdmB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     886.2; E-value: 4.1e-268)"
                     /gene_kind="biosynthetic"
                     /note="modifying enzyme"
                     /product="GdmB"
                     /protein_id="ABC94903.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 1.7e-122,
                     bitscore: 399.1, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 4.3e-16,
                     bitscore: 49.1, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 3.1e-20, bitscore:
                     62.4, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRTPLFSIETYKHHLNSNDINYNLLLKNGIIKESIMTTTYNLYK
                     SISKIDFEKNNKKARNAKESILKYLIRMSTRSTPYGMLSGVALGEFTENNNIKIKDPSC
                     HKKDVKVDGQWLYQLVHHLESDYTYYKDSIVIWNQQNYIYNNRLYLDNNSSITKNKDND
                     ALSVKYNTILMFIHEKSYENITYKELIHLISNEFNVENKEDVKLFIQELINKEILFSNL
                     RPGLENKNPLDYIIKNLNSKDSLVESLINTSNEIKKYSKIPLGEGESNYLYIINLMSKL
                     FDSKNYLQIDTYVDYSKNELKKSLANNISEAAYVLWLLSPNHFGTKTIRNYHEFFMDKY
                     GFEQLVNVKQLLSDINGFGYPQKANYSFSNNIAFLKEKYLLAIQNHSHIEITETDIKKL
                     EKNNTASETDAPVSTEIYSEIYFGNLIDGYDEYAVISPILGSFNSGATFGRFTGAFDTK
                     KRNQLEEEIVQRYNHYMNENDLKISQLNEAPLNSRNVNILNNNKIFDTSLNLNLPKSDV
                     DINDIFVGATFNKLYLYSEKYNSRILFISNSMFNYEFGSELYKFLREVSFEKTKFIQPI
                     TEESIDTLPFCPRIIYKNIILKPATWKLNLEMFSESKDWINTFTTIRKKWGIPENVIIA
                     FGDNRLLLNLLNDKHLVILKKELKKHGRIRILESYINKSDNKRMLEIVTPLYKKTHLKE
                     KHFIIPKNRNKNFHSLRDWFSIHLSIPKTYQDNFIRDYLFPFITELKVNKHINEFFYIK
                     FKKDEDFIKLRLFREDEDYSQIYPFIKKWKDYCLLNSELNDYSIVDYIPEVYRYGGPHV
                     IENVEKFFMYDSLLSINIIQTEFEFSKEFIVAMSIEFLIEQLNISDSEKKEILINNAED
                     LYRNNEIREYKNLLAKLTNSKNNYQFLKEEIPKLHEFLFNKISILEILKHTLQENLYTS
                     RSRIIGSFIHMRCNRIFGINPEKEKFVLSIFNEITKTKKYWDGRD"
     gene            8863..10119
                     /gene="gdmC"
     CDS             8863..10119
                     /codon_start=1
                     /gene="gdmC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     376.7; E-value: 2.2e-114)"
                     /gene_kind="biosynthetic"
                     /note="modifying enzyme"
                     /product="GdmC"
                     /protein_id="ABC94904.1"
                     /sec_met_domain="LANC_like (E-value: 4.3e-81, bitscore:
                     261.8, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVININNTKTILEKEIAFLSDVEQAIYTIENQSEYWDPYTLSHG
                     YPGIMLFLHESKEVLNKDLENTIHQYILKLGSYLENGIDGFSLFSGLSGIGFAIDIASD
                     KQYSYQGVLKQIDHLIIQYVIDFFNNNELEVTPTNYDVIQGLSGIGRYLLNRSSYNDDA
                     KKVLKNILNYFKKIHYSKNNWLVSNKHQFLDIDKQNFPSGNINLGLAHGILGPLSLVAM
                     SKMKGIEIENHEQFLYDFTSFLLKSEFKHNDEWLDRYDILEKYTPPYSVRNGWCYGDTG
                     IMNTLLLSGEALNDSELINTSKNILLTIIEKNNEDLISPTFCHGLASHLTIIHQANKYF
                     NLSQVNTYLETIVEKIVDHYSEENNFMFQDIEYSYGKKIYKNKVGILEGQLGVLLSLLD
                     YIDTQNRSRKNWKDMFLIM"
     gene            10135..10680
                     /gene="gdmD"
     CDS             10135..10680
                     /codon_start=1
                     /gene="gdmD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Flavoprotein"
                     /gene_kind="biosynthetic-additional"
                     /note="modifying enzyme"
                     /product="GdmD"
                     /protein_id="ABC94905.1"
                     /sec_met_domain="Flavoprotein (E-value: 1.3e-27, bitscore:
                     85.8, seeds: 143, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHGKLLICATASINVVNINHYIVELKQYFEEVNILFSPSSKKFIN
                     TDVLNLFCDNLYDETQDPLLNHINIVENHEYILVLPASADTINKIASGICDNLLTTVCL
                     TGYKSLYIFPNMNIKMWENPFLQKNIDLLKNNSVKVYPPDVNKSFEISSGLYKNSITMP
                     NIENVLSFILNSEKRPLD"
     gene            complement(10677..11294)
                     /gene="gdmQ"
     CDS             complement(10677..11294)
                     /codon_start=1
                     /gene="gdmQ"
                     /note="putative transcriptional activator"
                     /product="GdmQ"
                     /protein_id="ABC94906.1"
                     /transl_table=11
                     /translation="MIAINIVGEVDSTLTESILELDRRITINSSNIDPDFIIVYEKFDE
                     YYMFLKESIEKSPIIVVTGNNSYSRECYFYSLGIDLYIVRNKEISSIIICRVLNEIKKH
                     IKYIDDELIDFENQQFVFNNRLIKLSNIELKILQHLYSNLERYVSKDDLKKNVWDTEDF
                     VDSNTINVYIHRLRDNLRVCKEIEIINERKIGYKLLIKKDLC"
     gene            complement(11306..12691)
                     /gene="gdmP"
     CDS             complement(11306..12691)
                     /codon_start=1
                     /gene="gdmP"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S8"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1075:alkaline serine protease, subtilase family
                     (Score: 252; E-value: 2.4e-76)"
                     /gene_kind="biosynthetic-additional"
                     /note="putative serine protease"
                     /product="GdmP"
                     /protein_id="ABC94907.1"
                     /sec_met_domain="Peptidase_S8 (E-value: 1.5e-48, bitscore:
                     155.1, seeds: 43, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKFKFIFIFLMLAFVFVQNDYTFGSSYNEGLSYYSVEYNNERVF
                     NESIKQKDIELTYKIPEIHTAQIKTSKSQVESLIKNNKNIRFINPTCSTCVAKKSVQVE
                     KNSINKENGINDLFDKQWDMRKVTNEGKSYKLSSERKNAKVALIDSGVNSNHPDLKSIN
                     KTINEVPKNGFRGSEKDESGNRNFEKDKLNHGTLVAGQIGAKGSLKGVNPGVEMNMYRV
                     FGSKKSEMLWVSKGIVDAAKDDNDIINVSLGNYLIKDNQNNNKLRDDEKVDYDALQKAI
                     NYAQKKGSIVVAAVGNDGINVKDAKEINKKRNVESKSSKKVYDTPSNLNNVVTVGSINA
                     NNRIPEFSNYGSGFIDLTTIGGSYELLEKYGERVWLEEGYMQKQSVLSTSSNGRYIYQS
                     GTSLAAPKVSGALALEIDKYKLKDNPEKTLEIFKKKGIEKEKGMDKKHYGRGKLDVYKL
                     LKD"
ORIGIN
        1 tcgatacgat gagagacagc gtaaaatgtt tattgagata cttgctaaaa gttttatttg
       61 gattatctat tgtatgttat ttacattatt aattagattg attggtttaa caccaaatat
      121 tgaaaaaact tggttttctc aatatccaga aattttcttt atagtagctg ctatttactt
      181 atggctttta attactttac tacaaataaa aaaatacaca tctaaaggtt aaattaatga
      241 aaaatagaat tagagaatat agaaagcaaa aaagaatttc tcaagaagct ttaagtaagg
      301 aattaaaagt atctaggcaa actattaatg ctatagaaaa caataaatat gatcctacat
      361 tgacattagc atttaagttg gcaaaattgt ttgatacaac tgtagatgaa ttattttctt
      421 aaccattcat ttatcacaaa aagagagtgc aaattcgaac tctctttttg tgtagtagta
      481 ctattaaatt ttgtctcaat aacgatggtt aaaaatcatt gttgagaatt atgttaataa
      541 agtattgtag gcatagtgtt cttttcaaaa taagaaattc aatcttgaat acttctacct
      601 gaccaatttt taactaagac taaaagacat atgaacatca ttattatact tataatataa
      661 atagacattg ttaaaaagaa ggggactgaa ttgtttataa acatggaagt atatctagtt
      721 gaataggtga taggaatata ataccaagat ttatcaccaa gcatttctat accaaaataa
      781 atattaaata ttgtaattac aacccctgat accattgaga aaatactacc cgtaaataaa
      841 ctaattatga atattaatag tattataatt atattcataa aagaactact agctacataa
      901 aacaaagtta gttttacatt gtgagtcatc acaataaagt taactgttga gattaataag
      961 caaaataaat agttgataaa tatatagcta agtgttgcgc aaacccataa gcttctcttg
     1021 gatttgccta gtatatggtt aaaatgacct atactttctt caaaatgtat tacttgatat
     1081 gtagtaatag caataattaa agaagataac gcattaaaac tagaggtata ttgagaaata
     1141 gaagtataat tccagttacc tcctaagtaa agattaataa aagtaatata aaaaattgtg
     1201 aacaatgtgg gagtataggt tagtatctga gtacgaaatt ttaaattaat tacttttagt
     1261 tcatttttca tttatattcc aacttttctt agtatttgac ataaagaaaa tagaaaaata
     1321 aattatacta attattatgg ttaaagataa cgaatttaag tcgttaaaat atgggctatc
     1381 cttactcata agagtaccat ttgggtttaa tgataaagta attagtggaa ttcttccaat
     1441 atatgaccaa ggtagaatcc aaaagaattt agtaatagtg ataaagacaa tactgattat
     1501 agttaacgca atgtttaaaa aaatagaaaa aaacacgcca aaacgcttaa caaacaaaaa
     1561 attgagcggt atcagcggaa gacttacaaa aaagactaaa aacaacgtga ctaatacaag
     1621 gaataaacta actgaatttc caccaaccaa taaattccct atcattgcga caaataacat
     1681 tattatagta tggaataata aagcaatact cataactaat attttacttt taatcatctt
     1741 attaatcgac cagttattac ttacataata attgaataag ctatttcttt tctctatacc
     1801 aatcatcgag ctacaagaaa tgcatataat aattggcatg acaaaatatg gccacaantt
     1861 aaataataga gatangaatg gagataaatc atgttggtta ggactgcttt gagcaaataa
     1921 agcagtaaat aatgcgaatg ctaaaaaaaa caaaggtgat aaaaataata tatacatagc
     1981 agtagtattt ttgaacttta aagcctcagc ttttaataaa ctcatactat ctaccccctt
     2041 ttgttatatt aaagaatatt tcttcaagat tttcatcagt tttatttttt tcttgatatt
     2101 ctaacttacc attatgaata attcctatat gatcagctaa ttgctgtatt tcagaaagga
     2161 tgtgacttga aataataaca ctggtatttg cctttgttaa gttttttaat aagtctctta
     2221 attcttgaat gccataagga tctagcccat tagatggttc atctaaaact aacaacttcg
     2281 gtttctttat tagtgccata gctataccta aacgttgttt catccctaaa gaaaaatttt
     2341 tcaccttttt tttaggatca acatctaagt taacaagatc aagaacttta ggtatttcac
     2401 tgaagtcgat attttcattc aatgctacaa cttttaagtt gtcttgtgcg ctcaaatgat
     2461 tatatgttgg aggagattct attaaagaac caatattggt taattcttgt ctggttaagg
     2521 gtgaaccgtc taaatagaca tcaccatttg tctgtggcaa tattccacat ataattttca
     2581 tcaaagtaga tttaccagct ccattaattc ctaaaagtcc ataaacttca ttaggataga
     2641 cttttaaatt gatgtctttt aaaatagttt cttttttaaa ttgcttagtt aatttttggg
     2701 tttctatttt ataataatcc acaatttaat cctcctttaa gatttaattt attaaagttt
     2761 aacatttaaa aagtatatat agcctaacaa tattgttaga attactaata tgtaatttta
     2821 tattggaata caagaatgtg atatatttgt agaaaataga gttaaagata agggggacat
     2881 atatgtatct tttaatatta cttttattga ttctaattat aactactgtg attttgttta
     2941 aaaagcatag tagacttaac atttatattg tagctacagc acaacttatt ttcggtttac
     3001 tatttttcta ctatatatca gtaatattta actttagtat ttatattata gcgtctatgt
     3061 ttattatact ttccttatca atactagtat tcatagcttt tttaattttt aatagtaaaa
     3121 aacttcttga aaaagaagga agtagattat caaacctttt gagccttggt ttatctttgt
     3181 tgttattatg gataatagtc acatcccctg ttgctttttt tataaataaa tctttagcat
     3241 catatatatt tgtagtatat atctttattg tatctttact tatatatttt cttagtttat
     3301 ttattagttg ctttatttta tctctatttt attcaagaag aaaggtcaat aataatataa
     3361 gatatatttt agttttaggg tctggattaa tagaagataa ggtgcctcct cttttgcaaa
     3421 gtaggataga tattggtaaa aactttttcg actctaaaaa agacgctgat ataaaatttt
     3481 tattgagcgg aggacaaggt aaagacgaaa gaatttcaga agcacaagca atgaaaaatt
     3541 acttgatatc acaaggagta aatgaaaaca agataatatg cgaaaataaa tcacaaagta
     3601 catatgaaaa tttacagttt tcaaagaaat taattaaaaa aaatactaat atcttaatat
     3661 gctcaaataa tttccatata ctaagagcag tattcatagc taaaaaactc aaataccaac
     3721 atttttatag tctaggtagt aaaaccaaaa attatttctt tactaatgct tttattagag
     3781 agcttattgc aatattaaag atggattggg tatttcatct atgtataatt atatcactta
     3841 tattttggat ggttttcacc ttgtttaaat tataatagac tttaggcaat ttaattaaat
     3901 tgcctaaagt ctattaattt aacattacta ttagaaaaat catttttatc atgtctatgc
     3961 gttacattta agacaataga agtttgagct aactcctcaa tagatttaat tatgtattta
     4021 tgagattctt tatctaaatt agaagtgact tcatctagta aaatgatagg ttttttttgt
     4081 aaaaaagcgc gagttagtac taacctttgt ttttgtccaa ttgatagatt gatactatct
     4141 ggggaaattt gggtattaaa accatttttt aaattttgaa taaaaaataa agaattagtc
     4201 atttcgcagg cagctcgaac ttcttctata tttttagttt catcaattcc ataaatcaag
     4261 ttattataaa ttgtatcttc tagaatttga agatcttgtg ttatataact aaataagctt
     4321 ctccattcgt atttagagaa ctgctcctta tcaaaatcat ctaaatatat atttcctgta
     4381 taacctgtat ttaatcccgc caatatattt aatagcgtac tttttccaca accactttca
     4441 cctatgatat tgtaaatata acctttatag aaattcaaac ttatagaatt tattatctgc
     4501 ttgttttcta ttgtaaagtt caaatcattt attttcactt tttgagcaac attattataa
     4561 gctgtgtcga cagtaaaaac ttcttcttgc tctttattta tttttaagat tctatctgaa
     4621 gctccaattg ctcgttgcac ctctgtccaa taagatgtga tggatactat tggattaatt
     4681 atttgaaata aatataagat gtaagcgaac atatctccac ttttcatcac attattttcc
     4741 attaggtaat aaccaaagaa taaaatgcca aaaatattaa caaatagaag caagttcata
     4801 attggctcaa aaaaagacaa tactttaatt ttatgtaact ctatatcaaa caattttttt
     4861 aatagagagt aattatgttt cctttcaacg ttatatgtgc tcaaagtttt aattaacttt
     4921 attatagata atctattact ataataagaa gacaagttag cagttgcttc ttgggatttg
     4981 cttgatactc ttttcattac tttacctata ggtaacatta caattaccaa tattggtaat
     5041 gtacatatta aatatagagt caaagtttta ttgattacaa ataaaaaagt cagcgatact
     5101 ataactgaaa ataaatttgt caaaaaagta gatagttctt cagaaaaaat tctagttatg
     5161 ttcatagtat cgtttactaa tctactagtt aaattactag ctgaattttt taagtgaaaa
     5221 tcataaggta attttattaa cttattccaa gtaactattc taatattttg tattattttt
     5281 tgacctatat atcctaaaat ataagtagaa acaccagaaa aaattaatgt caatccaaag
     5341 cataaaatga taattacaac agtattagtt gaaatactag attcattcaa gtcatttcta
     5401 attattaaag gaatatataa tgaaacgcta gttccaatca aactaaatag taatccaatg
     5461 cttaaaagta tggtattagg cttggttatt ttccatagct catataaacc tctaataata
     5521 tcatcacctt tcaaactata tactattagt atatagtttg attacacaag aaaaaattac
     5581 acttttgtaa ttttctgaat aatatatagt aatttctttt ttatagtact aaaataacaa
     5641 ccgaaaagga ttgtataatt ctttttaata aaatttttag gagtgtttaa aatggaagca
     5701 gtaaaagaga aaaatgaact ttttgatctt gacgttaaag taaatgcaaa agagtctaat
     5761 gattcaggcg cagaaccacg aattgctagt aaatttttat gtactcctgg atgtgccaaa
     5821 acaggtagct tcaatagcta ctgttgttaa ttcaaaagaa ttaaattggc agagtctcaa
     5881 tagaggctct gccttaattt tgaggtgaaa taaaattgga taatatattt ggaccatcaa
     5941 atttatacat gctaagaacc ccattatttt caatagaaac atataaacat catttaaatt
     6001 ctaatgatat aaattacaat ttacttttaa aaaatggaat aattaaagaa tctataatga
     6061 caactacata taatctttat aaaagtatta gtaaaataga ttttgaaaaa aataataaaa
     6121 aagccagaaa tgcgaaagaa agcattttaa aatatctcat aagaatgagt actagaagca
     6181 cgccatatgg aatgttaagt ggtgtagctt taggagaatt cacggaaaat aataacataa
     6241 aaattaaaga cccttcatgc cacaaaaaag atgtaaaagt agacggtcaa tggttatacc
     6301 aattagttca tcatttagaa agtgattaca catattacaa agacagtatt gttatatgga
     6361 atcagcaaaa ttatatttac aacaaccgtt tatatttaga taataattca tcaataacca
     6421 aaaataaaga taatgatgca ttatctgtca agtacaacac tatattaatg tttatacatg
     6481 aaaaatctta tgaaaatatc acttataaag agcttataca tctgatatca aatgagttta
     6541 acgtagagaa taaagaagat gtgaaattat ttattcaaga acttataaac aaagaaattt
     6601 tattttctaa tttgcgacct ggattagaaa ataaaaaccc tttggattac attattaaaa
     6661 atctaaactc taaagacagt ttagttgaat cacttattaa tacttcaaac gaaataaaga
     6721 aatattctaa aattccttta ggggaaggtg aatctaatta tttatatatt attaatttaa
     6781 tgtctaaatt atttgactct aaaaattatt tgcaaataga tacatacgtg gattactcaa
     6841 aaaacgaatt aaaaaaaagt ttagctaata atattagtga agctgcatat gttctctggt
     6901 tattgtctcc gaatcacttt ggtacaaaaa caattagaaa ttatcatgaa ttttttatgg
     6961 ataaatacgg atttgaacaa ctagtaaatg taaagcaatt actctctgat ataaatggct
     7021 ttggttatcc tcaaaaagct aattatagtt tttctaataa tattgcattt ctaaaagaaa
     7081 agtacttact tgctattcaa aatcatagcc atattgaaat aacagaaact gatataaaaa
     7141 aactagaaaa aaacaataca gcttctgaaa cagatgcacc tgtttcaact gaaatttata
     7201 gtgaaatcta tttcggaaat ttaatagatg gttatgacga atacgccgtt ataagcccaa
     7261 tattaggttc ttttaattca ggtgctacct tcggaagatt cactggtgct tttgatacaa
     7321 aaaagagaaa tcaattagag gaagagattg tacaacgtta caatcattat atgaatgaaa
     7381 atgatttgaa aataagtcaa ttgaatgaag cacctcttaa ttcacgaaat gtaaatattt
     7441 tgaataacaa taagatattc gatacttcct taaatttaaa tttacctaaa agtgatgtag
     7501 atataaatga tatatttgtt ggggccacat ttaacaaact ttatctgtan tccgaaaagt
     7561 acaattcaag aattttattc atatctaatt caatgtttaa ttatgaattt ggatctgaat
     7621 tatataaatt tttaagagag gtttcgttcg aaaaaactaa attcatacag cctataactg
     7681 aagaaagtat cgacacattg cctttctgtc caagaattat ttataaaaat attattttga
     7741 aaccagctac ttggaaatta aatttggaaa tgttctctga atctaaagat tggataaata
     7801 cattcacaac tattagaaag aaatggggta ttcctgaaaa tgtaattatt gcttttggag
     7861 ataatcgatt actattaaat ttattgaatg acaagcatct cgttatatta aaaaaggagc
     7921 taaaaaaaca tggaagaatc cgaatattag agagttatat taacaaatct gataataaac
     7981 gaatgttaga aattgttaca ccattatata agaaaaccca tttaaaagaa aaacatttta
     8041 ttatacctaa aaatagaaat aagaacttcc atagtctcag agactggttt tcaatccatt
     8101 taagtattcc taaaacatac caagataatt tcattagaga ttatctattt ccttttataa
     8161 cagaactaaa agtgaacaaa catattaatg aattttttta tataaaattt aaaaaagatg
     8221 aagattttat aaaattaagg ttatttagag aagacgaaga ttattctcaa atttatcctt
     8281 tcataaaaaa atggaaagat tattgtttat taaatagtga attaaatgac tattctatag
     8341 ttgattatat tcccgaagta tacagatatg gtggccctca tgtcattgaa aatgtcgaga
     8401 aattctttat gtatgatagt ttactctcaa taaatataat acaaacagag tttgaatttt
     8461 caaaagaatt tattgttgct atgtcaatag aatttttaat agaacaatta aacattagtg
     8521 acagtgagaa aaaagaaatc ttaattaaca atgctgagga tttatatcgt aacaatgaaa
     8581 tcagagaata taaaaatttg ttagctaaac tgactaattc aaaaaataat tatcaatttt
     8641 taaaagaaga aataccaaag cttcatgagt ttttatttaa taaaatttca attttagaga
     8701 ttcttaaaca tacattacaa gaaaatttat atacgtctcg ctctcgaata attggtagtt
     8761 ttattcacat gcgctgtaat agaatatttg gaattaaccc tgaaaaagaa aaatttgttt
     8821 tatctatttt taacgaaatt acaaaaacta aaaagtactg ggatggtcgt gattaatatt
     8881 aataatacta aaacaatttt agaaaaagaa attgcctttt tgtctgacgt tgaacaagct
     8941 atatacacta tagaaaatca aagtgaatat tgggatcctt atactctatc tcatggttac
     9001 ccaggtataa tgcttttttt acatgaatca aaagaagtct taaataaaga tctagaaaat
     9061 actatacatc aatatatttt aaaactaggt agttatctag aaaatggcat tgacggattt
     9121 tcacttttca gcgggctttc aggcatcgga tttgcgatag atattgcgtc agataaacag
     9181 tattcttatc agggagtctt aaaacaaatt gatcacttaa ttattcaata cgttattgat
     9241 ttttttaata ataatgaatt agaagtgact cctactaatt atgatgtaat acaagggctt
     9301 tctggaatag gaagatatct attaaataga agctcatata atgatgatgc aaaaaaagta
     9361 ttaaagaata tacttaatta ttttaaaaaa attcattatt ccaaaaataa ctggttagtt
     9421 tcaaataagc atcaattttt agatatagat aaacaaaatt tcccttcggg gaatatcaat
     9481 ttaggattag cacatggtat tttaggtcct ctttcattag tagctatgag taaaatgaag
     9541 ggaattgaaa tcgaaaatca tgaacagttt ttatatgact tcacttcatt tttactaaag
     9601 tctgaattca aacataatga tgagtggtta gatcgctatg atatattaga aaaatacacc
     9661 cctccttatt ctgttagaaa tggttggtgt tacggtgata caggtattat gaacacttta
     9721 cttttatctg gcgaagcatt aaatgatagc gaattaatta atacttccaa aaatatttta
     9781 cttactataa tagaaaagaa taatgaagat ttaatcagtc caactttttg ccatggacta
     9841 gcttcacact taactattat tcatcaagct aataaatatt ttaacctatc gcaagtaaat
     9901 acttatcttg aaaccattgt cgaaaaaatt gttgatcatt attctgaaga aaataatttt
     9961 atgtttcaag atatagaata ctcatatggt aagaaaattt ataaaaataa agttgggatt
    10021 ctagaagggc aattgggtgt tcttctatct ctattagatt atattgatac acagaatcga
    10081 agtaggaaaa attggaaaga tatgttttta ataatgtaat aggaggagta aggtatgcat
    10141 ggaaaattat tgatatgcgc tacagcatca ataaatgtgg ttaatattaa tcactatata
    10201 gttgaattaa agcaatattt tgaggaagtt aatattttat ttagtccgag tagcaaaaaa
    10261 tttataaata ctgatgtact caatctattt tgtgataatt tgtacgatga aactcaagat
    10321 cctcttttga atcatattaa tatcgtagaa aatcatgagt atattttagt attacctgct
    10381 tctgcagaca ccattaataa aatcgctagc ggcatatgtg ataatctttt aactactgta
    10441 tgtttgacag gatataaaag tctttatata tttccaaata tgaatataaa aatgtgggaa
    10501 aatccattct tacaaaaaaa tattgattta cttaaaaata atagtgtaaa agtatatccg
    10561 cctgatgtga ataaatcatt tgaaatatct agcggactct acaaaaacag cataacaatg
    10621 ccaaatattg aaaatgtatt aagttttata ttaaattccg aaaaaagacc tttggactaa
    10681 caaaggtctt ttttaatcaa aagtttatat ccaattttac gttcattaat aatttctatt
    10741 tctttgcaca cccttaaatt atctcttaat cgatgtatat atacatttat tgtattagaa
    10801 tcaacaaagt cttctgtatc ccaaacattt ttctttaaat catctttcga cacatatctt
    10861 tcaaggttag aatataaatg ttgtagaatt ttcaattcta tattagaaag ttttattaaa
    10921 cgattattaa atacaaattg ttgattttca aagtctatca attcatcatc aatgtattta
    10981 atatgctttt ttatctcatt caaaactcta catattatta tgctgctaat ttccttattc
    11041 cttacaatat ataaatctat tcctaaacta taaaaataac actctctact ataactatta
    11101 ttacctgtca ctacaattat tggtgatttt tctatagatt cttttaaaaa catataatac
    11161 tcatcaaact tctcatatac aattataaaa tcaggatcta tattcgaaga attgattgta
    11221 atccttctat ctaattctaa aatactttca gtaagagttg aatctacctc acctacaata
    11281 ttaatagcaa tcatattatt tcccctcaat ctttaagtaa tttgtatacg tccagttttc
    11341 ctcttccata atgtttttta tccatacctt tttctttttc aatccctttt ttcttaaata
    11401 tttctaaagt tttttcagga ttatctttca atttatattt atcaatctct aaggctaatg
    11461 caccagaaac ttttggagct gcaagtgatg ttcctgattg atatatgtat cttccattag
    11521 aagatgtact taaaacactt tgcttttgca tatatccttc ctctaaccat actctttctc
    11581 catatttttc taaaagttca tacgaacctc cgatagttgt taaatctata aaaccacttc
    11641 cataattaga aaattcagga atacgattgt tagcattgat agatcctaca gtcacaacgt
    11701 tatttaaatt ggatggagta tcatatacct tttttgatga tttactttct acattcctct
    11761 tcttatttat ttcttttgca tcttttacat taataccatc attacccaca gctgcaacaa
    11821 caatactacc ttttttttga gcataattta tagctttttg tagcgcatca taatctactt
    11881 tttcatcatc tcttaattta ttgttatttt ggttatcttt aattaagtaa tttcctagac
    11941 taacattaat tatatcgtta tcatcttttg ccgcatcaac aattccctta gaaacccaaa
    12001 gcatctcgct tttttttgat ccaaacacac gatacatatt catctctact ccgggattta
    12061 caccttttaa actaccctta gctcctatct gtcctgctac taatgttccg tgattcaact
    12121 tatctttttc aaaatttcta tttcctgatt catccttttc gctacctcta aaaccgtttt
    12181 tggggacttc atttattgtc ttatttatac tttttaaatc cggatggtta ctatttacac
    12241 ctgagtctat taaagcaact tttgcattct ttctttctga acttagctta taacttttac
    12301 cttcattagt tactttccgc atatcccact gtttatcaaa taaatcattt ataccatttt
    12361 ctttattaat tgagttcttt tctacctgta cagatttttt agctacacaa gtagaacatg
    12421 tagggtttat aaacctgatg tttttattat tttttattaa ggactctact tgggacttac
    12481 tagttttaat ttgtgctgta tgtatttctg gaatcttata agttaactct atatcctttt
    12541 gtttaataga ttcattaaaa actctttcat tattatattc aacactataa taacttagcc
    12601 cctcattata tgagcttcca aaagtataat cattttgaac aaacacaaat gccaacatta
    12661 aaaaaataaa aataaattta aatttcttca tatagcaccc ctcac
//
