LOCUS       BGC0000515             12631 bp    DNA     linear   BCT 31-JAN-2014
DEFINITION  Geobacillus thermodenitrificans NG80-2, complete genome.
ACCESSION   BGC0000515
VERSION     BGC0000515
KEYWORDS    .
SOURCE      Geobacillus thermodenitrificans NG80-2
  ORGANISM  Geobacillus thermodenitrificans NG80-2
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
COMMENT     ##antiSMASH-Data-START##
            Version      :: 6.2.0-924c3663(changed)
            Run date     :: 2022-11-07 19:44:30
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12631
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..171
                     /codon_start=1
                     /gene="sunA"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TIGR03731"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mature_a"
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="GTNG_0265"
                     /product="Lantibiotic antimicrobial precursor peptide"
                     /protein_id="ABO65649.1"
                     /sec_met_domain="TIGR03731 (E-value: 3e-23, bitscore: 71.1,
                     seeds: 23, tool: rule-based-clusters)"
                     /sec_met_domain="mature_a (E-value: 2.4e-08, bitscore:
                     22.7, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKFDDFDLDIVVKKQDDVVQPNVTSKSLCTPGCITGVLMCLTQN
                     SCVSCNSCIRC"
     CDS_motif       1..69
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MAKFDDFDLDIVVKKQDDVVQPN"
                     /locus_tag="GTNG_0265_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       70..171
                     /prepeptide="core"
                     /locus_tag="GTNG_0265_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class I"
                     /score="17.80"
                     /molecular_weight="3263.9"
                     /monoisotopic_mass="3261.5"
                     /core_sequence="VTSKSLCTPGCITGVLMCLTQNSCVSCNSCIRC"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MAKFDDFDLDIVVKKQDDVVQPN"
                     /leader_location="[0:69](+)"
                     /alternative_weights="3281.9"
                     /alternative_weights="3300.0"
     protocluster    1..12631
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[945:7358]"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      946..7358
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-i"
                     /protocluster_number="1"
     cand_cluster    1..12631
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((LANC_like and (Lant_dehydr_N or
                     Lant_dehydr_C)) and not (YcaO or TIGR03604))"
                     /kind="single"
                     /product="lanthipeptide-class-i"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..12631
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000515"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..12631
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-i"
                     /region_number="1"
                     /rules="((LANC_like and (Lant_dehydr_N or Lant_dehydr_C))
                     and not (YcaO or TIGR03604))"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            1..171
                     /gene="sunA"
                     /locus_tag="GTNG_0265"
     gene            946..4086
                     /gene="spaB"
                     /locus_tag="GTNG_0266"
     CDS             946..4086
                     /codon_start=1
                     /gene="spaB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: Lant_dehydr_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lanthipeptide_LanB_RRE"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1155:Lantibiotic dehydratase domain protein (Score:
                     1048.1; E-value: 0)"
                     /gene_kind="biosynthetic"
                     /locus_tag="GTNG_0266"
                     /product="dehydratase"
                     /protein_id="ABO65650.1"
                     /sec_met_domain="Lant_dehydr_N (E-value: 3.4e-152,
                     bitscore: 497.1, seeds: 73, tool: rule-based-clusters)"
                     /sec_met_domain="Lant_dehydr_C (E-value: 3.3e-38, bitscore:
                     121.3, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Lanthipeptide_LanB_RRE (E-value: 7.2e-20,
                     bitscore: 61.0, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDLVFKNIDTFMIRTPVLSVDNYLRFFDQKLTEGEMKERLLEIC
                     HNPVFRESILVASKSLYNKMIDFCNGKEIKKYDYFIKAIYKYLIRISTRPTPFGLFAGV
                     DFGEYTDENTSIRYGTNKYKKFARPDLEWLMKIIKKLEQEQYEQLWFTVNDSIFLKGER
                     AYLLHSTRKDDDKRVNEISVRVTLPFKITCELARHLIHYQTLKKELIKQFPNTSEEKIE
                     RFLKQLIENEFLISNLRPPLTVMDQLDYLIKRLKESHIEEWSNELIDIQQKIRTYTMTP
                     LGEGEQIYKELHKKMKKLADTKNVLQVDMKLNLQEKKLNKQVIKDVNELMHILLPFSMT
                     YQQTDSPLSRYKQEFIEKYGVDREVPLLEMLDNDLGIGAPMDYTNPKNARLAVGVPHQL
                     IDEQLREYFLHKYIEAVKDKKSITITEDEIKELGLDDYDYQSIPDSLDINVIVKCDSEQ
                     DLENNHFKYYIGPNLGSTHAGKSFGRFSYMMEGEEKLFETLNKEMLNLNNDSGEYVTCE
                     LVYLPNETRHANVTRNIHNSEYELALFTNNSKDEYHRLSLNDILIGIENDMFYAKSKTL
                     NKKLIITMNNMLNTQNAPNAIRFLYDISLDGKKIWCNFPWDTIFQDYAYIPAIEYKNFV
                     IAPRKWVINKIIKTNDKISFTEFEKRFKKFCSDYDVPKYVYLTFADNRILLNTEDENCL
                     RILHHEFNNRYGNIILSSYEEADLHLVKDDDEKEYVCELVIPLVKVKQENRQWYAKAKK
                     SNHIPSFSDIRVKLPFENWLYLKLYGVHSRADDFIAFYLSEYCREKLAKGEIEKYFFIR
                     YADPEQHIRLRLNASEEKLLQIYPDMKQWLRTLIEKRVVSRFSIDCYEREIERYGGTEL
                     IDLAENLFFFDSIVVEDILKLKRLKHIQFSDEVIGMVSIIHYMEQFGLNYESQLDFLQQ
                     QVNKQDYREDFQKNRSVYMKVCNTDNDWQGLRDTDEGMLLLNVLNQRNRSIKEYASKIQ
                     DGLEASSELSILDSVVHLHCNRLFGIDREFEKKIRTLVAHTLYALKYIKAHGLVLKN"
     gene            4253..6082
                     /gene="spaT"
                     /locus_tag="GTNG_0267"
     CDS             4253..6082
                     /codon_start=1
                     /gene="spaT"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 179.2; E-value:
                     1.7e-54)"
                     /gene_kind="transport"
                     /locus_tag="GTNG_0267"
                     /product="Putative SpaT"
                     /protein_id="ABO65651.1"
                     /transl_table=11
                     /translation="MNIKEYDDHVTIKDIVKSIRIVPKTLVLIKNVDKQSFFIIIFLSL
                     LMGGAPIVTLLGSQNLLNAIGSQNIHIIISALIFYISAVLLSEIISSFMEYYQNKFQTL
                     INYKLNLQIMDKCTRLSLRHFEDAEVYDMLQRVQNETPYKPFEVFLSILGAISAIVTFF
                     SSMMILIHWKPWVLIILIFIPLVFSFYFFKIGQREFNVSWLRAPEKRKSWYLSYLMTRD
                     NTFKEVKIYNLAGYILNKFKEINKTFVGQDIHLFKRRTVFTFIFELVEQICINTVLVII
                     IISALAGEILIGHVVGLIQALNLISSNSKKILNIIHSLYKNNLYINQLFEFLGLDEEVL
                     DKGDRFVSGIDKIENIKIRNLTFRYPTNPKVVLKNINLDINKGERIAIVGANGSGKSTL
                     IKLLLKLYEHDEDSIFYNGVSINDLDTEQLRKNIAVLFQDFVKYELKLRENVGFGDITR
                     IDRDGELKQAMERARVNFIEDLDTQLGLWFVDGVQLSGGQWQKVAIARALFRDASVYIL
                     DEPSSALDPISEKEVIDMFINMTKDKIGIFISHRLSTAKLADKIVVMNDGEIVGIGTHW
                     ELLATNELYRRMYELEALKNEEEYEHLNESAGL"
     gene            6051..7358
                     /gene="spaC"
                     /locus_tag="GTNG_0268"
     CDS             6051..7358
                     /codon_start=1
                     /gene="spaC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-i: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1140:Lanthionine synthetase C family protein (Score:
                     420.8; E-value: 9.3e-128)"
                     /gene_kind="biosynthetic"
                     /locus_tag="GTNG_0268"
                     /product="cyclase"
                     /protein_id="ABO65652.1"
                     /sec_met_domain="LANC_like (E-value: 3.4e-89, bitscore:
                     288.3, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSISMKALVCNLAKKLSDYENMKRIVNHPSNYIKIGNKTINPFNE
                     LSLSHGLPALCALYGELSEQYPDEGWDLLGHQYMKKIGEQMNQHGFPSLSMFTGLAGIG
                     LAAVCLSKGGKRYQNFISTINQVIEERIGEMIEYLKQKPYPNMDDYDAISGVAGIASYC
                     LLFPQEMKKSITLILKYIIDLSQDKQMEDINVPGWYIPPMNAFSDTERRKWPSGFFNIG
                     LSHGIPALLIVLCNAKKLNIYVHGQDECIQRIADFLMKFQIKDENGSYWGTHVSLEEYK
                     NGSVLNKDTRDAWCYGTPGVAYSLLIAGKTLNNQSYIDCAVSGMKLASKRLYNIFSPSF
                     CHGLSGVAYICNRFYEETNISDFKEAACKLVDDIIKFYNEEFPFGFKNIEESEGSTKYY
                     DYVGLIDGTAGILLTILAIQNSKKTPWDCAFLLSEV"
     gene            7701..8399
                     /gene="spaR"
                     /locus_tag="GTNG_0269"
     CDS             7701..8399
                     /codon_start=1
                     /gene="spaR"
                     /gene_functions="regulatory (smcogs) SMCOG1008:response
                     regulator (Score: 199.9; E-value: 6.7e-61)"
                     /gene_kind="regulatory"
                     /locus_tag="GTNG_0269"
                     /product="Subtilin biosynthesis regulatory protein SpaR"
                     /protein_id="ABO65653.1"
                     /transl_table=11
                     /translation="MNNKKILVVDDDEDICCTIKQYLELHHYQVETVHSGVEALKMIND
                     DFDLIVLDIMMEGIDGMELCTIIRDRVDYPIVFVSAKTLEEDKVQALAIGGDDYITKPF
                     SLKELKARIDSHLRREERIRSNERYLLSSKNITIDLLANEVFCKGKKLPLTKKEYEIVK
                     LLMLNKKVVFSKERIFERVWGIDSDSQLDTVTESIKNIRKKIKAVDPETSYIQTLYGLG
                     YKWDVTDEKE"
     gene            8386..9804
                     /gene="spaK"
                     /locus_tag="GTNG_0270"
     CDS             8386..9804
                     /codon_start=1
                     /gene="spaK"
                     /gene_functions="regulatory (smcogs) SMCOG1003:sensor
                     histidine kinase (Score: 188.5; E-value: 4.6e-57)"
                     /gene_kind="regulatory"
                     /locus_tag="GTNG_0270"
                     /product="Sensory transduction protein kinase SpaK"
                     /protein_id="ABO65654.1"
                     /transl_table=11
                     /translation="MKKNKTIKRDFYLLVVKVVLATAISSAVTYLCLIYLIITLATNDV
                     VKPTNYFVKDLDLIEKKVKENEEAIFKGRLIPIHRYSEKIQGEVVDISGKHLYGDREII
                     DDQVDISQSINREIVKGSYVYRFIPLKHDNAIQAVYVLKAPFGFIINNQNRFEAVLIYA
                     VMFISPLIFFIVYLIFFTSRLYKSLFHNVKLLLQASDHIASGNFDFQVSGLKRKEFIKI
                     QDSFNTMISALKEMVERLAKTDDERKMMVSSIAHDIRTPLTVIQGQIDLIQDLRKLDHF
                     DVTPHLEIIRKNCGKMTMLTDNLSLLYKVENDHFSLNGKEVDVRQILEEKKLEISTMVY
                     HQKSINICFDVNLQKSRYILDEAMLMRVLDNILYNSLRFTKSGEIKLEVYDEKEGNECK
                     IYFRCSDTGTGFKENDTSLLFQPFYQDKQYKNHVGLGLYIAKRIVNHHGGEIWAYNNEK
                     GGATVEFYIKELSD"
     gene            complement(9898..10332)
                     /gene="spaI"
                     /locus_tag="GTNG_0271"
     CDS             complement(9898..10332)
                     /codon_start=1
                     /gene="spaI"
                     /locus_tag="GTNG_0271"
                     /product="SpaI"
                     /protein_id="ABO65655.1"
                     /transl_table=11
                     /translation="MKNSILLLMLIGILFIGGCSLVSDLKKTATQNMEIDRKLPKYELN
                     KENLQEIHYQGRTYMIQAAKVDRNQLNKPIGKVAETITINEHHQILSKKELRKIEVIPD
                     QTDEKRTHLNFGWVYSIKGVNPDEEVAVTVNHQFLIAKRK"
     gene            complement(10387..11001)
                     /gene="spaG"
                     /locus_tag="GTNG_0272"
     CDS             complement(10387..11001)
                     /codon_start=1
                     /gene="spaG"
                     /locus_tag="GTNG_0272"
                     /product="SpaG"
                     /protein_id="ABO65656.1"
                     /transl_table=11
                     /translation="MAVSLPIIISLLTGLMSYQEEKAGSFMNILTGPVSKVQWYVGKLT
                     LLIFIACADILLATILMLFGMKYVLKVAHIHYQLFLQGAMLSIVGSLILFSLHLFISFA
                     FGMGASIAVGSGGFLISALIGATSLGDHVWSYIPWAWAVRLSQFPILMMPEVKQALGAQ
                     LLNSFSLSLWEGIAYALLSFIIVTIIGSIWFHRWEGNQPHE"
     gene            complement(11177..11923)
                     /gene="spaE"
                     /locus_tag="GTNG_0273"
     CDS             complement(11177..11923)
                     /codon_start=1
                     /gene="spaE"
                     /locus_tag="GTNG_0273"
                     /product="Putative SpaE"
                     /protein_id="ABO65657.1"
                     /transl_table=11
                     /translation="MLNMFESERLKYKRTFAKKLVFIAPLFFVLYAIITMPTVNSTHNY
                     FEYAVFNWWPLIFVPIGTALIASLSTVREKKSGNDKVLRCHDISIVRLWWSKITVIAYY
                     MLLSTIELIILLVLLKWLFPNSLSSATVAVFASLVIWLTSLAYIPIGLFVAERFGMVVS
                     IIVSVIGLALGVVMAAQPYWLFIPWSWGMRMMCPIVGVHPNGVPLEKGSPLLDPSVIPI
                     GLAIALVMFLLLSFLTAVWFARKEVY"
     gene            complement(11942..12631)
                     /gene="spaF"
                     /locus_tag="GTNG_0274"
     CDS             complement(11942..12631)
                     /codon_start=1
                     /gene="spaF"
                     /gene_functions="transport (smcogs) SMCOG1000:ABC
                     transporter ATP-binding protein (Score: 185.5; E-value:
                     2e-56)"
                     /gene_kind="transport"
                     /locus_tag="GTNG_0274"
                     /product="Putative ABC transporter ATP-binding protein"
                     /protein_id="ABO65658.1"
                     /transl_table=11
                     /translation="MGEYMLETHHVSKAFGKQLVVDDVSIKVKKQTVYGLLGPNGAGKS
                     TLLKMLTGLLRPTKGEIMINGHPWSRKDLKDIGVLIESPALYGNLTAHENLLVHAKLLH
                     LPESRIQEVLNIVGLENTGKKKASQFSLGMKQRLGIAKALLNHPKLIILDEPTNGLDPL
                     GIQDLRKLIQSFTENGITVILSSHILSEVKQLADYIGIIQNGKLRYEGKIDESQDLEKL
                     FVDVVNE"
ORIGIN
        1 atggcgaagt ttgatgattt tgatttggac attgtggtga aaaaacagga cgacgtggta
       61 caaccgaacg tgacgagtaa atctctttgt acgccaggtt gtatcacagg tgtgttaatg
      121 tgtctcacgc aaaattcttg tgtatcttgt aactcttgca ttcgatgcta atggtttcga
      181 ttgatcacac attcattgaa ccatgtgagc gttaaatcac gctaacaagg tcgatcatag
      241 gctaacttcc gacaaaaaag aaaaagggaa tttggcactt acattcaccc ttgatcgaca
      301 atcgtcaagg aggagaagga ttagtaagga caagggcaga aataaagagg ataagaagcc
      361 cttgtccatt cttgattttt ccttagatca tcttggaatg gtccatgact cattatatct
      421 cgggtgagac agacatgata gggccacccg atgtttttga tgacatcaag cataaaaaac
      481 aagcaatgct aatatctttt gacttgcata tacatctcgc ccgctcagaa tgaaacagac
      541 cggattattt tatgaaagaa aaaactgggc gataaaaggg attggagtga taacgtttgc
      601 tacatgtagt tgcgttaggc acgactgatt tgcggcccta ccccatcaga atgttgtgaa
      661 cccacagaca ggtgtcatta ttcctcagtc tgttgtttca aaaacaaagt cgataagttt
      721 gaaggaaggg caaactgctc aaacatgaag tatcacaaca atttttgaaa caccccgcaa
      781 aaaccaaatg tcgtttcgaa agcaacacaa gattgggccg ccagctttct gcttcgtacg
      841 aggcagaagg tgtcagctgc tttagtatgg aatgtatgta tagttccgta tagaatcccc
      901 ttttaactta tggagatggt gaatgattga gtaggaggct ttattatgaa cgatcttgtc
      961 tttaaaaaca tcgatacgtt tatgattcga acaccggttt tatcagtaga caactattta
     1021 agattttttg atcaaaaatt gactgaggga gaaatgaaag agcgtctgtt ggaaatttgt
     1081 cataaccctg tatttagaga gagtatctta gtagcgagta aaagtttata taataaaatg
     1141 attgattttt gcaacggcaa ggaaataaaa aaatacgatt attttatcaa agccatttat
     1201 aagtatttga taagaattag cacgagacca acgccatttg gtctttttgc aggagtagac
     1261 tttggtgaat acacggatga aaatacatca ataagatatg gaacgaataa atataagaaa
     1321 tttgctaggc cagatttaga atggctgatg aagattataa agaaattgga acaagaacaa
     1381 tatgagcaat tatggttcac ggtgaatgac agtatatttt taaaaggaga acgggcatat
     1441 ttactacatt cgacacgaaa agacgatgac aagcgagtca atgaaatcag cgtcagagtc
     1501 acccttcctt ttaaaatcac gtgtgaatta gctcgtcact tgatccatta ccaaacatta
     1561 aaaaaagaat taataaaaca atttccaaat acgagtgagg aaaaaattga acgttttctc
     1621 aagcaactaa tagaaaacga atttttaatt tccaatctcc gcccaccatt aacagtaatg
     1681 gatcagcttg attatctgat aaagcgattg aaagaaagcc atatagaaga gtggagtaac
     1741 gaattaattg atattcaaca aaagattcgc acatatacca tgaccccgtt aggagaaggg
     1801 gagcaaatat ataaagagtt acataaaaaa atgaaaaagt tagccgatac caaaaatgtg
     1861 ttacaagttg atatgaagtt aaacttacaa gaaaagaaac tgaacaagca agtgataaaa
     1921 gatgtaaatg aactaatgca catacttcta cctttttcga tgacctatca acaaacagat
     1981 tctccacttt cgaggtacaa gcaagaattt atcgaaaaat atggggtgga cagagaagtt
     2041 cctttactag aaatgcttga taatgatctt ggtataggag cacctatgga ttatacaaac
     2101 ccgaaaaacg caagactcgc tgtaggtgtg ccccatcaat tgattgatga gcagttaaga
     2161 gaatattttc ttcataaata tatcgaggcg gttaaagaca aaaaaagtat aacaataaca
     2221 gaagacgaga taaaagaatt gggtctagat gattatgatt accaaagcat cccggattca
     2281 ttagacatta acgtcattgt aaaatgtgac tcggaacaag atcttgagaa caatcatttc
     2341 aaatactata ttggccctaa tttaggatcg acccatgcgg ggaaaagttt tggaaggttt
     2401 tcttatatga tggaagggga agagaaactc ttcgaaacgt taaataaaga aatgttgaac
     2461 ttgaataatg atagtggcga gtatgtaact tgtgagcttg tttatttgcc aaatgaaacg
     2521 agacatgcca atgtgacaag aaatattcat aacagcgaat acgaacttgc gttgtttact
     2581 aacaacagca aagatgaata tcatcgattg agcttaaatg atattttgat cggcatagag
     2641 aacgatatgt tttatgccaa aagcaaaaca ctaaataaaa agttgattat tactatgaat
     2701 aatatgttga atacacaaaa tgccccgaat gcgattcgtt ttttgtatga tataagctta
     2761 gatggcaaaa aaatttggtg taatttccct tgggatacaa tctttcaaga ctatgcttac
     2821 ataccggcaa ttgaatacaa aaatttcgtc atagcaccgc gaaaatgggt gatcaataag
     2881 attattaaaa caaacgacaa aattagtttt acggaatttg aaaaacgctt taaaaaattt
     2941 tgttctgatt atgatgtgcc gaaatatgtt tatttgactt ttgcagataa tcggattttg
     3001 ttgaatacag aggatgaaaa ttgtttaaga attcttcatc acgaatttaa caatagatac
     3061 ggaaacatta ttctaagttc ttatgaagag gctgatcttc atttagttaa agatgatgac
     3121 gaaaaagagt atgtttgtga attggtcatt ccgttagtga aagtgaaaca agaaaatcgt
     3181 caatggtatg ccaaagcgaa aaaatcaaat catatcccaa gtttttcaga tataagagtc
     3241 aagttgcctt ttgaaaattg gctttattta aaattgtacg gtgtccattc tagagcggat
     3301 gatttcatcg ccttttatct ttcagaatat tgtcgcgaaa aattggctaa aggagaaatt
     3361 gaaaaatatt tctttatacg gtatgccgac ccggagcaac atataagatt aaggctgaat
     3421 gccagtgaag agaaacttct acagatttat ccggatatga aacaatggct gcgaacgtta
     3481 atcgaaaaaa gagtggtgag tcgtttttcc atcgactgct atgaaagaga aattgagcgt
     3541 tatggtggaa cagaattgat cgatcttgca gaaaacttat ttttcttcga tagcatagta
     3601 gtagaggaca tattaaagtt aaaacgtttg aaacatattc aatttagcga tgaagtgatc
     3661 ggaatggttt ccatcattca ttatatggaa cagttcggcc taaattatga aagtcaattg
     3721 gatttcttgc aacagcaagt caacaaacaa gactatcgtg aagattttca aaagaatcga
     3781 tctgtttaca tgaaagtttg taatacggat aatgattggc aagggttaag agacactgat
     3841 gaggggatgt tattattaaa cgttttgaac caaagaaaca gaagcataaa agagtatgca
     3901 agcaagatcc aggacggtct cgaagcttct tctgaattat ctattttaga cagtgtggtt
     3961 catttacact gtaacaggct attcggcatt gatcgagaat ttgaaaagaa aatccgtaca
     4021 ttagttgccc atacgttata tgcattaaag tatattaaag ctcatggatt agtgttaaaa
     4081 aattaaagat gttatccatt tgtccaagca ttggggagac gatatataaa tggacccacg
     4141 acttggactt ttttcaacaa aagctcgaca agctctcttc tgtgtattca ggtgaaaaaa
     4201 cttttatgac gggaagaaga tctcatttta gcatgcgggg attgaaaatg ttatgaatat
     4261 aaaagagtat gatgatcacg ttactatcaa agatattgtg aaatcaataa ggatagttcc
     4321 caagacgctt gttcttataa agaacgttga caaacaaagt ttttttatca ttattttctt
     4381 aagcttatta atgggaggcg caccgattgt tacattgctc ggttcgcaaa acttgttgaa
     4441 tgcaatcggg tcccaaaata ttcatattat tatatcggca ttgatctttt atatttctgc
     4501 tgttttactc agcgagatca tcagcagttt catggaatat taccaaaata agtttcaaac
     4561 tctcatcaac tacaaactta atttgcaaat catggacaag tgtacaagat tgtctttacg
     4621 gcattttgag gatgccgaag tttatgatat gttgcaaaga gtacagaatg aaacgccgta
     4681 taaaccattt gaagtgtttt tatccatatt aggtgcaata tcggcgattg taaccttttt
     4741 ttcatcgatg atgattctga tccattggaa accgtgggtg ttgattatat taatcttcat
     4801 accactcgtt ttttcttttt attttttcaa gattggccaa agagagttta atgttagttg
     4861 gttgcgtgca ccggaaaaaa ggaaatcatg gtatttgtct tacctaatga caagagacaa
     4921 cacgttcaaa gaagtcaaaa tatataactt ggcaggctat attttgaaca aatttaaaga
     4981 aatcaacaaa acgttcgtcg ggcaggatat ccatcttttt aaaagaagaa cggtttttac
     5041 atttattttt gagctcgttg aacaaatatg tatcaatacc gtacttgtta tcatcatcat
     5101 ttcggcgttg gcaggtgaaa tccttatagg tcacgtcgtg gggcttattc aggccttgaa
     5161 cttaatttct agcaactcta agaaaatatt gaatatcatc cattccttgt ataaaaacaa
     5221 tttatatatc aaccaactgt ttgaattttt ggggttagac gaagaagtat tggacaaagg
     5281 agatcgattc gtatcaggga tagacaaaat cgaaaatatt aagataagaa atttaacgtt
     5341 tcgttatcca acgaatccaa aagtagtatt gaaaaatatt aacctcgaca tcaacaaagg
     5401 ggaaagaata gccatcgtcg gtgcaaatgg atcaggaaaa agcaccctta taaaactttt
     5461 attgaaattg tatgagcacg atgaggatag cattttttat aatggtgtgt ccatcaatga
     5521 tttggatacg gaacaattga gaaaaaacat tgccgtgttg ttccaagatt ttgtgaagta
     5581 tgaattgaaa ttaagggaaa acgtggggtt tggcgatatt acgcgcattg atcgtgacgg
     5641 agaattaaaa caagccatgg agagggcgag agtaaatttt atcgaggatt tagatactca
     5701 gcttggttta tggtttgtcg acggtgttca gctttcggga ggacagtggc aaaaagtagc
     5761 gattgctagg gcgttatttc gggatgcatc cgtatatata ttggatgaac ctagctcggc
     5821 actggatccc atttccgaga aagaagtcat tgatatgttt atcaatatga caaaagataa
     5881 aattggtatc tttatctcac atcgtctatc aacagctaaa cttgccgata aaatcgtagt
     5941 aatgaatgat ggtgaaattg tcggtatagg tacgcattgg gaattgttag ctacgaatga
     6001 gttatacaga agaatgtatg aattggaagc tttgaaaaac gaggaggagt atgagcatct
     6061 caatgaaagc gctggtttgt aatcttgcga aaaaattgtc ggattatgag aatatgaagc
     6121 gaatcgtcaa tcacccaagt aattatatta aaattggaaa caagacgata aatccgttta
     6181 atgagttaag tttaagccat gggttaccag ccttatgtgc gctgtacggc gaattgagcg
     6241 aacaatatcc ggatgaaggt tgggatttat tagggcatca atacatgaaa aaaatcggtg
     6301 aacaaatgaa tcaacacggt ttcccttctt tatctatgtt taccggattg gcagggatcg
     6361 gccttgctgc agtatgttta tccaagggag gaaaaaggta tcaaaacttt atctcaacga
     6421 ttaatcaagt catcgaagaa agaatcggag aaatgatcga gtatttgaaa cagaaaccgt
     6481 atccgaatat ggacgattac gatgctattt cgggagtagc gggtatagcg agctactgcc
     6541 tattatttcc gcaagagatg aaaaaaagta ttacgttaat cttaaaatac attatagatt
     6601 tatctcaaga taaacaaatg gaggatatca acgttcctgg ctggtatatt cctccaatga
     6661 atgctttttc agacaccgaa aggagaaaat ggccatcggg tttctttaac attgggttgt
     6721 ctcacggtat acctgcttta ctcattgtct tgtgcaatgc caaaaaactg aatatttacg
     6781 tacatgggca agatgaatgc attcaaagaa tagctgattt tcttatgaag tttcaaatta
     6841 aagatgaaaa tggctcttat tgggggactc atgtttctct agaggaatac aaaaatggct
     6901 ctgtcctaaa caaagataca agggatgcct ggtgttatgg cacgcctggt gttgcttatt
     6961 cgttacttat agcaggtaaa actctaaata atcaatcata cattgattgt gctgtcagcg
     7021 gaatgaaact ggcaagcaaa cggctttaca acattttttc tccttccttc tgtcatggtt
     7081 tatcgggagt tgcgtatata tgcaacagat tttatgaaga aacgaatatc agcgatttta
     7141 aggaagccgc ctgcaaatta gtcgatgata taataaaatt ttataatgag gaattccctt
     7201 ttggctttaa aaatattgag gagagcgaag gaagcacgaa atattatgat tatgtgggac
     7261 taatcgatgg aacagcggga attttattga cgatattagc tatacaaaac tcgaaaaaaa
     7321 cgccttggga ttgtgcattt ttattatctg aagtttaaat gttgtttata tacaatggaa
     7381 aagccgactg atcgatggtt ggaggcaagc cgcagagggg gttccatcat accttggcgt
     7441 accgatcggt cgaacaactc cctaaaaagg gtgttgttcg atcgaccagt catcgatcaa
     7501 atatgttgga aaaggttcat agacaagagg cgtctttcac ccgggcgtca ctcaagacgt
     7561 gcgcattttt tagtaaaaag caacaaagag taaagcttca cattgcacct atgaacaaac
     7621 gcaacggtgt tatgagatta ccccatgata tggggatgga ataggttaaa atgcgattca
     7681 actgtatggg ggatcaaaag atgaataaca agaaaatttt ggtcgttgat gatgatgaag
     7741 atatttgctg caccattaaa caatatttgg agcttcatca ttatcaggta gaaacggtcc
     7801 acagcggcgt agaagcgttg aagatgataa acgacgattt tgatttgatt gtgttggata
     7861 tcatgatgga aggaattgac ggaatggagc tgtgtacaat cattcgtgat cgggttgatt
     7921 accctattgt atttgttagt gctaaaacgc tggaggaaga taaggttcaa gctctggcga
     7981 tcggtgggga tgattacatc accaaaccgt tcagcttaaa ggagttaaag gcgagaattg
     8041 attctcattt aagaagggaa gaacggattc gttcaaacga gagatatctt ttgagttcta
     8101 aaaatattac gattgatctt ttggctaacg aagtcttttg taaaggaaag aaattaccgt
     8161 taacaaaaaa agaatatgag attgttaagt tgttgatgct caataaaaag gtcgttttct
     8221 ccaaagaaag aatctttgaa cgagtatggg ggattgactc cgacagccag ttagacactg
     8281 ttacagaaag cattaaaaat attcgaaaga aaatcaaggc cgtcgatcca gaaacctctt
     8341 atattcaaac attatatggg ctcggttata aatgggatgt gaccgatgaa aaagaataaa
     8401 acgattaaac gagattttta tttgttagtt gtgaaagtcg tgttggcaac ggcaatctct
     8461 tcggccgtta cttatttatg cttaatctat ttgattatta cattagccac gaacgatgtg
     8521 gtgaaaccga caaattattt cgtgaaggat ttggatttga ttgaaaaaaa ggtgaaagaa
     8581 aacgaagagg cgattttcaa ggggagatta attcccatcc atcgttacag tgaaaagata
     8641 cagggagaag ttgtagacat cagcggtaag catctgtatg gtgatcgaga aattatagac
     8701 gatcaagtgg atattagtca atcgatcaac cgtgagattg taaaaggaag ctatgtgtat
     8761 cggttcattc ccttgaagca tgataacgcc attcaggcgg tatatgtgtt gaaagcgcct
     8821 tttgggttca tcattaacaa tcaaaatcgc ttcgaagccg tgttgattta tgctgtgatg
     8881 tttatttctc cgttgatctt ttttattgtc tatttgattt tctttacatc taggctgtac
     8941 aaatcattgt ttcataatgt caaactgttg ctccaggctt cggatcacat tgcaagcggg
     9001 aattttgatt ttcaagtctc ggggttgaaa agaaaagagt ttatcaagat ccaggattcc
     9061 tttaatacca tgatcagtgc actgaaagaa atggtggaac gtttagcaaa gaccgatgac
     9121 gaacgaaaaa tgatggtatc atcgatcgca catgatatca gaaccccgtt aacggtcatc
     9181 caaggacaaa ttgatttgat tcaagatttg agaaagttgg accactttga tgtcactccc
     9241 catttagaga ttattcgcaa aaactgtggg aagatgacca tgttgacaga taatttgtcg
     9301 ttgctctata aagtggagaa tgaccacttc tcattgaatg gaaaagaagt ggatgtaagg
     9361 caaatccttg aggagaaaaa actagagatc tcaacaatgg tttaccatca aaaatcgata
     9421 aacatttgct ttgatgtgaa tttgcaaaaa tcacgttaca tacttgatga agcgatgtta
     9481 atgagagtgt tggacaatat tttatacaac agcctgaggt ttacaaaaag cggagaaatt
     9541 aagttggaag tctatgatga aaaagagggg aatgagtgta aaatctattt tcgctgttca
     9601 gatacaggaa cggggtttaa agaaaacgat acgtctttgt tgttccagcc tttttaccag
     9661 gataagcagt ataaaaatca tgttggatta ggactgtata ttgccaaacg aattgtgaat
     9721 catcatggcg gtgaaatttg ggcctacaac aatgaaaaag gcggagcgac ggttgaattt
     9781 tatattaagg agctatctga ttaatcatcc aatcttatgg gctttccgcc gttatagaga
     9841 gtggagaatg cagctaggga ggaatttctc tttccctagc tgacagtctc taagccttta
     9901 ttttctttta gcaatcaaaa attggtggtt caccgtaaca gccacttctt catctgggtt
     9961 tacgcctttg atgctgtata cccaaccaaa gttcaagtga gttcgttttt catccgtttg
    10021 atcgggaatg acttcgatct ttcgcagctc ttttttactc aagatttgat gatgctcatt
    10081 gatggtaatc gtctccgcta ctttcccgat cggtttgttc aattgattcc gatcgacttt
    10141 tgctgcttga atcatgtacg ttcggccttg atagtgaatt tcttgcaaat tttctttgtt
    10201 caactcgtat tttggtaatt tccgatcgat ctccatattt tgtgtggctg tcttttttaa
    10261 gtcactgaca agtgaacaac ctcctataaa gaggattcca atcaacatga ggagtaagat
    10321 ggagtttttc atcgttactc tgaaacctcc ttttttacca tcccattgag agggagcagt
    10381 gaactttcat tcatgtggtt gatttccttc ccaacgatga aaccagatcg aaccgatgat
    10441 ggtgacgatg atgaagctta gaagggcgta ggctattcct tcccataatg atagcgaaaa
    10501 ggaatttaac aactgcgccc ccaaggcttg ctttacttcg ggcatcataa gaatcggaaa
    10561 ttgtgagaga cgaacagccc aagcccaagg aatatagctc catacgtgat cgcctaagga
    10621 agttgcgcca atcagggcgg aaatgagaaa tccgccgctt cctactgcga tcgaggctcc
    10681 cattccaaag gcaaagctga tgaacagatg caaggaaaaa aggatgagtg agcccacgat
    10741 ggagagcatg gctccttgca aaaataactg ataatgaatg tgcgccactt tgagtacgta
    10801 tttcattcca aagagcatga gaatcgtcgc tagcaagatg tccgcgcatg caataaagat
    10861 gagcagcgtg agtttgccaa cataccattg aactttggat acaggaccgg tcaagatgtt
    10921 catgaaactt cctgcttttt cttcttgata ggacatcagc ccagtcagta gggagataat
    10981 gatcggtaag gaaacggcca ttacttcaaa aaatccttga tagattttca tctgccaaaa
    11041 cgtagaggta cggtagtgcg aaaaatacca taccataaag agcgaataaa tgattggcgt
    11101 gatgatgacc atcatccgaa tcgctgtgcg ttttgttttt atccattcgg atgacagaag
    11161 gcgaagaaac cacatgttaa tacacttcct ttcgcgcaaa ccaaacggcg gttaaaaaag
    11221 ataacaatag aaacatgaca agggcaatcg ccaggcctat tggaatgaca gaaggatcca
    11281 atagcggact ccctttttcc aaaggaacac cgttcggatg aaccccgacg atcgggcaca
    11341 tcatcctcat tccccagctc catgggatga acagccaata cggttgtgcg gccatgacta
    11401 cccctaaggc gaggccgatg acactgacaa tgatcgagac taccatcccg aaccgctcgg
    11461 ccacaaataa tccgatcggg atataggcta aagaggtcaa ccaaatgacc aagctggcga
    11521 atacggcgac cgtagctgat gagaggctgt tgggaaaaag ccatttcaat aaaacaagca
    11581 agatgatcag ctctattgtt gagagcaaca tgtaataggc gatgacggtg attttgctcc
    11641 accatagacg cacgatgcta atgtcgtgac aacgcaatac tttatcgttt cctgactttt
    11701 tctcccttac cgttgacaag gaagcgatca aggctgttcc aatcggaaca aaaatcaatg
    11761 gccaccaatt aaatacggca tattcgaaat aattgtgtgt ggaatttaca gttggcatgg
    11821 tgatgatggc gtacaggaca aagaaaagcg gagcgatgaa tacaagtttt ttcgcaaacg
    11881 tccgcttata ttttaaacgt tctgactcga acatattgag catgatttcc atcctccttc
    11941 attactcgtt cactacatcg acaaagagtt tttcgagatc ttgactttcg tctattttcc
    12001 cttcgtacct gagttttccg ttttggataa tgccaatata atcggctaat tgcttaactt
    12061 cggacaaaat atggctcgat agaatgacgg tgatcccgtt ttcggtaaac gattgaatga
    12121 gttttcgcaa gtcctgtatg cctaatgggt ccaagccgtt tgttggttcg tcgagaatga
    12181 tcagctttgg atggtttaaa agcgctttgg caattcctaa tcgctgtttc attccgaggg
    12241 aaaattgaga tgctttcttt ttccctgtat tttctaagcc gacgatgttc aaaacttctt
    12301 ggatccgcga ctcaggcaaa tgaagcaatt tagcgtgaac aagcaagttt tcatgagccg
    12361 ttagattgcc gtaaagagca ggggattcga tcagcacacc gatatctttt aagtcttttc
    12421 tgctccaagg atggccgttg atcatgattt cccctttcgt aggacgcaaa agccctgtca
    12481 gcatttttaa aagggttgac tttccggctc catttggtcc gagcaatcca taaaccgtct
    12541 gtttcttgac tttaatggat acatcatcta cgacgagttg tttcccaaac gctttgctta
    12601 catgatgggt ctctaacata tactctccca t
//
