LOCUS       BGC0000516             10920 bp    DNA     linear   BCT 15-AUG-2008
DEFINITION  Geobacillus thermodenitrificans strain G11MC16 ctg51, whole genome
            shotgun sequence.
ACCESSION   BGC0000516
VERSION     BGC0000516
KEYWORDS    .
SOURCE      Geobacillus thermodenitrificans
  ORGANISM  Geobacillus thermodenitrificans
            Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
COMMENT     URL -- http://www.jgi.doe.gov
            JGI Project ID: 4043128
            Source DNA and Bacteria available from David Mead
            (dmead@lucigen.com)
            Contacts: David Mead (dmead@lucigen.com)
            David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Annotation by JGI-ORNL
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-10-17 13:46:25
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 10920
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..1173)
                     /codon_start=1
                     /locus_tag="G11MC16DRAFT_3399"
                     /note="KEGG: gtn:GTNG_2060 hypothetical protein"
                     /product="conserved hypothetical protein"
                     /protein_id="EDY04297.1"
                     /transl_table=11
                     /translation="MTTLFVNEVKNQSIDIGDMDRYHFEFLQPILEHKRFVFLGESSHC
                     VKEYSLAKVSLIKYLHEELGFHVLAFESELGPCMIGDYLSDQLDSKRFMAGTIGRVWQN
                     EFVLGLFDYMKKMKQERPLYFTGVDVYQGKKENLFSEFLESYLTGPTKQKFVDFEKRAI
                     EVLIAADKRKIKRSMRQNIREEMESKGMELIEELKQHCFPDSKIYKIVLRTMENRVNYL
                     KATLEKSFSKLFEYRDALMAKNLEFLAQEIYPNEKFIIWAHNMHISKNTSAKRLSAYKS
                     FVENLSQTIKEKSFVLGLYAKEGKMLDYTGKEYPIKKMNKKHLESLLDHSPYQNSFIQC
                     NGNWAQKKWRAYEGGGMPTSFVPAQQYDGILFFKYVSPAYFDVSEKNREE"
     protocluster    1..10920
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3368:6449]"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3369..6449
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(LANC_like and DUF4135)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-ii"
                     /protocluster_number="1"
     cand_cluster    1..10920
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(LANC_like and DUF4135)"
                     /kind="single"
                     /product="lanthipeptide-class-ii"
                     /protoclusters="1"
                     /tool="antismash"
     subregion       1..10920
                     /aStool="externally annotated by: MIBiG"
                     /contig_edge="True"
                     /label="BGC0000516"
                     /subregion_number="1"
                     /tool="antismash"
     region          1..10920
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-ii"
                     /region_number="1"
                     /rules="cds(LANC_like and DUF4135)"
                     /subregion_numbers="1"
                     /tool="antismash"
     gene            complement(1..1173)
                     /locus_tag="G11MC16DRAFT_3399"
     gene            complement(1174..3339)
                     /locus_tag="G11MC16DRAFT_3400"
     CDS             complement(1174..3339)
                     /codon_start=1
                     /db_xref="InterPro:IPR001140"
                     /db_xref="InterPro:IPR003439"
                     /db_xref="InterPro:IPR003593"
                     /db_xref="InterPro:IPR005074"
                     /db_xref="InterPro:IPR011527"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288:ABC
                     transporter related protein (Score: 267.7; E-value:
                     3.7e-81)"
                     /gene_kind="transport"
                     /locus_tag="G11MC16DRAFT_3400"
                     /note="PFAM: ABC transporter transmembrane region; ABC
                     transporter related; peptidase C39 bacteriocin processing;
                     SMART: AAA ATPase; KEGG: gtn:GTNG_2061 lantibiotic
                     mersacidin transporter system"
                     /product="ABC transporter-related protein"
                     /protein_id="EDY04298.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 3.4e-36, bitscore:
                     113.1, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTKRRVPLKRQIAQTDCAFCCLSMIVAYYKSYVSFHELQERMGG
                     GRDGVSLHALSEIASNIGFNTQVYKRKDLFEVDSPVIVYWDHFHYVVLEKINKRGYHVL
                     DPAIGRCIYSKEEFHKHFTGYYLELKPNNNFKRKKPNNVWLPYLKIAFEKPKLLFSVLI
                     GSMFIQLLTIIMPLLTKFAIDEIVGLNHYEFFYNFLLSIIFLVLINGVIHFLRGRFLAN
                     LRVHLDWKLMSRFFKHLINLPFQYFQMRSFGDILYRANAHIMIRELFSQQTIMSFLDST
                     LILIILGYMAINSPWLTGWVILFGLLNVLLLTLTQNAIKQRSEEELSEQAKFQDAQVET
                     LYGIFGIKMAGVERQVFNQWERLLKNLLSAFKKKEYYSNKINACIVSLELAAPLIILAL
                     SLHQVQLGNITVGTMVAFYSLTGTFFGLVSSLVDTVQSMIMMDTYLHRINDVMEAPTEK
                     IPEKAIKLEKLSGNIRLENVSFSYTKYSRPVLKNISLEIKAGQKVAIVGPSGSGKSTLA
                     RMLLGLYEPNVGEIFYDNVNLKDIDKQWLRRKIGVVPPDATLFNKSILHNISVYEENPS
                     MDDVIEAAKIAQIHDEIMAMPMGYYTPVSEMSMNISGGQKQRIMLARAILQKPAILILD
                     EATSSLDNINEKNIDKHLNEMNCTRIIIAHRLSTIKDADCIIVLNDGKIVESGTHYELM
                     KKKGFYYNLYYSKSNNEELDAVLEGEI"
     gene            complement(3369..6449)
                     /locus_tag="G11MC16DRAFT_3401"
     CDS             complement(3369..6449)
                     /codon_start=1
                     /db_xref="InterPro:IPR007822"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: DUF4135"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-ii: LANC_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1070:lanthionine synthetase C family protein (Score:
                     1071.5; E-value: 0.0)"
                     /gene_kind="biosynthetic"
                     /locus_tag="G11MC16DRAFT_3401"
                     /note="PFAM: Lanthionine synthetase C family protein; KEGG:
                     gtn:GTNG_2062 lantibiotic mersacidin modifying enzyme"
                     /product="Lanthionine synthetase C family protein"
                     /protein_id="EDY04299.1"
                     /sec_met_domain="DUF4135 (E-value: 1.8e-123, bitscore:
                     401.4, seeds: 79, tool: rule-based-clusters)"
                     /sec_met_domain="LANC_like (E-value: 4.5e-77, bitscore:
                     248.2, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNEIVENNLMEIIKDKLLYPSEKKKEIENNVPINELLFQKKTNKW
                     IDLFYQYLNVCKNNIEVDQLYSNEENLSNPFSSFFKEFLEIASRYLDSSLKSQVNNYDD
                     LSRICNLTSIKNDIMKFIHENLVYVSIRTLIQDLNEEREKGNLIGDTPKERYNFYVDQI
                     LSSPDKKFELIKKYPVLVRILIEFILNKIDSIVESIYRFLKDRSELVHIFHLSDDDILT
                     SLTLQSGDSHNNGRSVIIFQFSSKKKIVYKPRSLSIDLHFQQFLEWINGKKPSLQLKTI
                     TILNKDQYGWQEFVEYKPCSSNNELSRFYERQGNYIAILYILNATDFHFENLIANGEHP
                     VLIDLEGLVQNTVKLPRKASSAYDIAFSKLTDSVLSTGMLPATFMQANIYDFDLSGLGG
                     DEGQPTGLETFTIENPLTDEMRVIKVPAFSQSSKNKPYLKEEKEIAVTDYSSEIIKGFR
                     EMYTLLLHNKKELLSQNGPIYLFKGDKVRIILRSTQVYSTFLDSSFHPDYLKDGYERER
                     LINFLWIGKENHPEYQDAIMYECRDILNGDIPYFYCYTDSSDLYHPIGIVKHNFFYESS
                     FNSLLEKVKMINEEDLNFQIEILTNSLLAQYSNKTHSHANVSNRVYNLDKISGNFRRES
                     FLEVSEKIADNIKENAIFGKENDVTWLGLNLTIEDKWTFNPLDFDLYDGVLGIGLFYAN
                     LYNLNKRKEYKILAEKTVQTALNYLEYYPAKLPLSAFYGYGAYAYVLGNFSIIFNNSEY
                     LTYVKKVLNKAANTIEDDQLLDFLGGAAGLIIVCIHLYKKTGEQYLLDIANKCGELLLR
                     KKEKQAMGIGWRPNQVNKPLAGLAHGSSGFTWALMALYKFTKNYNYKETFLQSFEYEKS
                     LFNKKEENWLVLSDDGDYYGNSMWCHGAAGIGMSRIMMSEYYNSHDLKNDIEIAIRQTL
                     KSGFGGTHCLCHGDLGNLDLFLLASSKFKNEEMKETALKIGEYIASDITYGNLKYGTPS
                     GTKNLGLMLGQAGIGYGFLRLAYPEIVPSVLTLQLNH"
     gene            complement(6534..6707)
                     /locus_tag="G11MC16DRAFT_3402"
     CDS             complement(6534..6707)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (lanthipeptides)
                     predicted lanthipeptide"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="G11MC16DRAFT_3402"
                     /product="hypothetical protein"
                     /protein_id="EDY04300.1"
                     /transl_table=11
                     /translation="MEKQEQTFVSKISEEELKKLAGGYTEVSPQSTIVCVSLRICNWSL
                     RFCPSFKVKCPM"
     CDS_motif       complement(6639..6707)
                     /prepeptide="leader"
                     /note="peptide class: {self.peptide_class}"
                     /note="predicted sequence: MEKQEQTFVSKISEEELKKLAGG"
                     /locus_tag="G11MC16DRAFT_3402_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(6534..6638)
                     /prepeptide="core"
                     /locus_tag="G11MC16DRAFT_3402_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class II"
                     /score="2.00"
                     /molecular_weight="3797.5"
                     /monoisotopic_mass="3794.9"
                     /core_sequence="YTEVSPQSTIVCVSLRICNWSLRFCPSFKVKCPM"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MEKQEQTFVSKISEEELKKLAGG"
                     /leader_location="[6638:6707](-)"
                     /alternative_weights="3815.5"
                     /alternative_weights="3833.6"
                     /alternative_weights="3851.6"
     gene            complement(6847..7518)
                     /locus_tag="G11MC16DRAFT_3403"
     CDS             complement(6847..7518)
                     /codon_start=1
                     /db_xref="InterPro:IPR000792"
                     /db_xref="InterPro:IPR001789"
                     /db_xref="InterPro:IPR013249"
                     /gene_functions="regulatory (smcogs) SMCOG1016:LuxR family
                     DNA-binding response regulator (Score: 140.2; E-value:
                     9.3e-43)"
                     /gene_kind="regulatory"
                     /locus_tag="G11MC16DRAFT_3403"
                     /note="PFAM: regulatory protein LuxR; response regulator
                     receiver; Sigma-70 region 4 type 2; KEGG: gtn:GTNG_2063
                     two-component response regulator ComA"
                     /product="two component transcriptional regulator, LuxR
                     family"
                     /protein_id="EDY04301.1"
                     /transl_table=11
                     /translation="MIHILVVDDHRLVGEGTKNMLESEQDFQVELVTSGTEAEQMMEQR
                     TYDVFLLDWRMPDMSGTELSKRILQKQPNAKIVIYTGYDVAPFFNYLIESGITGFVSKT
                     ATSEQLITAIRCALRDEAVIPVHLLRQLQLCEVKARMVENEEHVALSQLEQEILFEVAN
                     GLSNKDIAKKHYISQRSVERHLSRIFTKLHVSSRIEAVEKARQLGLIPELHMLLTPSSD
                     R"
     gene            complement(7544..9808)
                     /locus_tag="G11MC16DRAFT_3404"
     CDS             complement(7544..9808)
                     /codon_start=1
                     /db_xref="InterPro:IPR001478"
                     /db_xref="InterPro:IPR003594"
                     /db_xref="InterPro:IPR005467"
                     /db_xref="InterPro:IPR011712"
                     /gene_functions="regulatory (smcogs) SMCOG1048:sensor
                     histidine kinase (Score: 322.6; E-value: 6.9e-98)"
                     /gene_kind="regulatory"
                     /locus_tag="G11MC16DRAFT_3404"
                     /note="PFAM: ATP-binding region ATPase domain protein;
                     histidine kinase dimerisation and phosphoacceptor region;
                     KEGG: gtn:GTNG_2064 sensor protein ComP"
                     /product="integral membrane sensor signal transduction
                     histidine kinase"
                     /protein_id="EDY04302.1"
                     /transl_table=11
                     /translation="MRRTITVISVAAIVALYLTIFNVKHPLIGMDVEKNKNGDIVVSDI
                     YEFGWAKKNGIHINDQVLKVDGKPPLSHFTVRKYKTIEQAKTITIQRENQIQTLTVDYK
                     SHGTEQWLYYIALPMGFFLFAVSLSIFLLRLRPDDKSATVLIYFLLLIGLCYISASLSA
                     KYALFGRQMFNGIFLILPAVFLHFVHHYFEKEKKVWFAKKIVFSLYGFNLVMFVASLIS
                     LSFRSISEAEVLLATFFINMVIILALLGKGFVYLKKDEQQNTVKGLLLAIGLSAAPFVL
                     FYSLPVLIIGKWVLPAELTVTSLFVLPSVFLYLICTDRLFRLQFSINRFVYYAFLSFLP
                     AFLITLLYAKFVNEAWRLVEFALLFLLIYIINILALYLKECFDRTLRAKLYVQKDFYQE
                     SIYRIGETLKKQKNMQEVIRCMEKEVREVLDIENLAFVQLEVKNMQAPFHCDEKEWMKL
                     IKKMDGQPLSIGKIIENKRLFAVFVGSFRQSPLLLIGKKKHPIPFRAEQKDWLSTIVYY
                     ASVTIENMLKVEDFIKELDSLKERSNSVWLTRLIFQWSEKERRKLAIDLHDTILQDLIV
                     LKRKVESLRFQNMRMEEMKKQLNYIEEEIVDMIDLTREICHELRPPFFSQIGLKEAIVQ
                     LIQRFQLRVNIKVELNVHLSAKLKEERELAIYRIIQELLNNALKHSQASYIQLTLEAQN
                     EEVQLHYVDNGIGIDMETLNHCDGKLGLFGIKERVQGLGGTCEMTSQLGEGLDIRITIP
                     L"
     gene            complement(9815..9970)
                     /locus_tag="G11MC16DRAFT_3405"
     CDS             complement(9815..9970)
                     /codon_start=1
                     /locus_tag="G11MC16DRAFT_3405"
                     /product="hypothetical protein"
                     /protein_id="EDY04303.1"
                     /transl_table=11
                     /translation="MQEMIRFLMENPEVIEKLKSGTVSLVGLDELEVQAIIKVFSQSVT
                     PLGYWK"
     gene            complement(9988..10920)
                     /locus_tag="G11MC16DRAFT_3406"
     CDS             complement(9988..10920)
                     /codon_start=1
                     /db_xref="InterPro:IPR000092"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182:Polyprenyl synthetase (Score: 84.5; E-value:
                     1.1e-25)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="G11MC16DRAFT_3406"
                     /note="PFAM: Polyprenyl synthetase; KEGG: gtn:GTNG_2065
                     pre-ComX modifying enzyme"
                     /product="Polyprenyl synthetase"
                     /protein_id="EDY04304.1"
                     /transl_table=11
                     /translation="MQGVAEMIEKEAVKMQMKEIVTASFSVPSLKESVLTSIEEHTKQS
                     DFLFGRLAGVHYQMFSENMKETERIAASVELMMLAGDMLDDLVDQDSLETTWNKAPLTT
                     SLHIAIGLLLAGQKAIVDLLIDDHKKAKAQLYISSRLLQSVNGQHMDVTYDIQTEAEYI
                     EMVKKKSGSLVAMACFVGTALAGAEKNTVIIEKYANDIGIAAQIENDIDALYRWDEKND
                     IKAKKKSLPILYMLTSKRDNLLKQYFSNHVDYNELYVQKEKVIQQMEQEGAFYYAKVMS
                     QIYKEKALQEIEKLDVNDRYKSALKMIIL"
ORIGIN
        1 ttactcttct ctgttcttct cagaaacgtc aaagtaagct ggggaaacat atttaaaaaa
       61 taaaatccca tcatattgtt gtgcaggtac aaaggaagta ggcattcctc cgccctcata
      121 ggcccgccac tttttctgtg cccagtttcc attgcactga ataaaactat tttgataagg
      181 ggaatggtca agaaggcttt ccaaatgttt tttattcatt ttttttattg gatattcctt
      241 acctgtataa tccaacattt tgccttcctt tgcatacaac cccaatacaa aacttttctc
      301 cttaatcgtt tgggagaggt tttccacaaa cgatttatag gcagacaggc gcttggcgga
      361 agtatttttg ctaatatgca tgttatgtgc ccaaatgata aatttttcat ttgggtatat
      421 ttcctgtgct aaaaattcta ggttttttgc cattaatgca tcccgatact caaaaagttt
      481 cgagaaactt ttttctaaag ttgctttcaa gtaatttact ctattttcca tcgtacggag
      541 gacaatttta taaatcttgc tgtccggaaa acaatgctgt ttgagttcct caatcaattc
      601 catgccctta ctttccatct cttcccttat attttggcgc attgaacgct tgattttccg
      661 tttatcggct gcaatcaaaa cctcgatcgc ccgcttttca aaatcgacaa acttctgttt
      721 ggtcggaccc gttaaataag actcgaggaa ttccgaaaac aaattctcct tttttccttg
      781 ataaacatcc acaccagtaa aatacagcgg ccgctcctgc ttcatttttt tcatatagtc
      841 aaataatcca aggacaaatt cattttgcca cacacgccca atggtacccg ccataaaacg
      901 tttagaatca agttgatcag acagataatc gccgatcata catggcccca attcgctttc
      961 aaaggcaagt acatgaaacc ccaattcttc atgaagatat ttaatcaggc ttacttttgc
     1021 tagactgtat tctttgacgc aatggctgct ctccccaagg aagacaaagc gtttatgttc
     1081 caaaatcggc tgaagaaact caaaatgata gcggtccata tcccctatat cgatactttg
     1141 atttttgact tcgtttacaa ataaagtagt catttaaatt tcaccttcca aaacggcgtc
     1201 taattcctca ttattactct ttgaataata aagattgtaa tagaagcctt tctttttcat
     1261 taactcgtag tgagtccccg attcaactat ttttccatca ttcagaacaa taatgcaatc
     1321 cgcatcttta attgtgctta aacggtgcgc tattataata cgagtacaat tcatttcatt
     1381 taaatgttta tcaatatttt tctcattaat attatccaat gaacttgttg cctcatctaa
     1441 tatcaaaata gcaggttttt gtagaatggc tcttgctaac ataattcttt gtttttggcc
     1501 acctgatata ttcatcgaca tttcagagac aggtgtgtag taacccattg gcatagccat
     1561 tatttcgtca tgtatttggg caattttcgc agcttcaatt acatcatcca tagaaggatt
     1621 ttcctcgtaa actgaaatat tgtgaagaat ggatttgtta aaaagagtgg catctggagg
     1681 gacaactcca atttttctcc gtaaccactg cttatctata tcttttaaat taacattatc
     1741 ataaaatatt tcacctacat ttggttcata taaacctaat aacatccggg ccagtgtact
     1801 tttacctgat cctgatggtc caacaatcgc aactttttgc cctgctttta tttcaagact
     1861 gatattttta agtacggggc gactatattt tgtataagaa aagctaacat tttctagtct
     1921 aatattgcct gaaagttttt ctagttttat agctttctcc ggtatttttt ctgttggagc
     1981 ttccattaca tcatttatcc tatgtaaata agtgtccatc attatcatgc tttgtactgt
     2041 atcaactaaa gaagatacca gtccgaaaaa agtccctgtt aatgaataga atgccaccat
     2101 tgtcccaaca gtaatgtttc caagttgaac ttggtgtaat gataaagcta aaattattaa
     2161 aggagcagcc agctccaaac tcactatgca agcatttatt ttattagaat aatattcttt
     2221 tttcttaaat gcacttaata gattttttaa caagcgctcc cactgattaa aaacttgtcg
     2281 ttctacaccg gccattttga ttccaaaaat accatataat gtttcaactt gtgcatcttg
     2341 aaatttagct tgttcagata attcctcttc ggagcgctgc ttaatcgcat tttgtgttaa
     2401 ggttaataat aatacattca ataacccaaa taaaattacc catcctgtta accaaggtga
     2461 atttattgcc atatatccaa gtatgataag aattaaagta gaatctaaaa aactcatgat
     2521 ggtctgttga gaaaaaagtt cacgtatcat tatatgggca tttgccctgt atagtatatc
     2581 tccaaatgat ctcatttgaa agtactgaaa aggtaaatta attaaatgtt taaagaacct
     2641 gctcattaat ttccaatcaa gatgaaccct taaattggct aaaaaacggc cgcgtaaaaa
     2701 atggattact ccattaatta aaaccagaaa aataatactg agaagaaaat tgtaaaaaaa
     2761 ctcgtaatga tttaagccaa caatctcatc tattgcaaat tttgtaagta aaggcattat
     2821 tatagtcagt aattgaataa acattgaccc aattaatact gaaaataata atttaggttt
     2881 ctcaaaggct atttttagat atggtagcca aacattgttc ggtttcttcc ttttaaaatt
     2941 attgttgggt tttaattcca aataataacc tgtgaaatgt ttatgaaact cctctttaga
     3001 ataaatgcat cgaccaatag caggatccaa cacatggtat ccccttttat ttattttctc
     3061 taataccaca taatgaaagt gatcccaata aacaatcact ggcgaatcaa cttcaaataa
     3121 atcttttctt ttgtaaactt gtgtgttaaa cccaatattg gaagctattt cggaaagggc
     3181 atgcaacgaa actccatcac gaccgccacc cattctctct tgtaattcat gaaagctgac
     3241 atagctctta taataagcaa caatcatcga caagcaacaa aaggcacaat cagtttgtgc
     3301 aatttgtctt ttaagcggta ctcttctttt tgttttcatt aatatattcc tccatataat
     3361 tcctcctatt aatgatttaa ctgaagagtt aaaactgatg gaacaatttc cgggtaagca
     3421 agccttaaaa aaccataacc tattcctgct tgtccaagca ttaagcccag attttttgta
     3481 ccggagggag ttccatactt taagtttcca tatgttatat ctgaagcaat atattctccg
     3541 atttttaatg ccgtctcttt catttcttca tttttgaact tgctagatgc taataggaac
     3601 aaatctaaat tacctaaatc cccatgacat agacaatggg tacccccgaa accacttttt
     3661 aatgtttgcc taattgcaat ttcaatatca ttttttaaat catggctatt ataatattca
     3721 ctcatcataa ttctgctcat tccaatgcca gcagctccat ggcaccacat cgaattacca
     3781 taataatctc catcatcact caaaactaac caattttctt cctttttatt aaatagagat
     3841 ttttcatact caaaagattg taaaaatgtt tccttgtaat tataattttt agtaaactta
     3901 tagagtgcca ttagcgccca agtaaatcct gaagatccat gtgcaagtcc agctaaaggt
     3961 ttattaactt gattaggccg ccatccaatc cccattgctt gtttttcttt ttttcttaac
     4021 aataactctc cacacttatt tgcaatgtct aataaatatt gttcgcctgt ttttttataa
     4081 agatgaatgc acactataat taaccctgct gcccctccta gaaagtccaa taattgatca
     4141 tcttcaatcg tatttgctgc cttatttaaa acttttttta cataagtaag atactcggaa
     4201 ttattaaaaa taattgaaaa attccctaat acatatgcat aggcaccata accataaaaa
     4261 gcagataacg gtaattttgc tgggtaatat tccagataat tcaaagctgt ttgtacagtt
     4321 ttttcagcta aaattttgta ttcttttctc ttatttaaat tatataagtt ggcatagaac
     4381 aaacctattc caagcacgcc atcatacaaa tcaaagtcaa gggggttaaa tgtccattta
     4441 tcctcaatgg tcaaatttaa ccccagccat gtaacatcat tctcttttcc aaatatcgca
     4501 ttttctttaa tgttgtcagc gattttttcg gaaacttcca aaaaactttc ccgtcggaaa
     4561 ttgccagata ttttatcaag attataaact ctatttgaaa catttgcatg actatgagtt
     4621 ttatttgagt attgcgctaa tagcgaattc gttaaaatct ctatttgaaa attcaaatct
     4681 tcttcattta tcatttttac tttttcaagc aaactgttaa aactggattc ataaaaaaaa
     4741 ttatgcttga ctattccaat tggatgatat aagtcgctcg aatcagtata gcaatagaaa
     4801 tatggtatgt caccattcaa tatatctcta cattcataca ttattgcatc ttggtattca
     4861 ggatgatttt ctttaccaat ccataagaaa tttataagac gctctctttc ataaccatct
     4921 tttagataat ccggatgaaa actcgaatct aaaaatgttg agtatacctg tgtacttctt
     4981 aaaattatcc ttactttgtc gcctttaaat aaatatattg gaccattttg cgataatagt
     5041 tcttttttat tatggagcaa taacgtgtac atttctctga atcctttgat gatttcggag
     5101 gaatagtctg taactgctat ttctttttct tcttttagat aaggtttatt tttactactt
     5161 tgtgaaaatg ctggaacttt aattaccctc atttcatcag ttagagggtt ttcaattgta
     5221 aacgtttcaa gaccagtcgg ctgtccttca tctcctccta aaccacttaa atcgaaatca
     5281 taaatatttg cttgcatgaa tgttgccgga agcatacctg ttgataagac tgaatcagtt
     5341 aatttactaa atgcaatatc ataggcggaa gatgcttttc gaggtaattt aacagtgttc
     5401 tgcactaatc cttcaagatc aattaacacc ggatgctctc cgtttgcaat taaattctca
     5461 aaatggaaat ctgtagcgtt caatatgtaa aggatagcaa tataattacc ttgccgttca
     5521 taaaaacgag ataattcatt attagaagag caaggcttgt attcaacaaa ttcctgccat
     5581 ccatattgat ccttatttaa aattgttatt gtttttaatt gcaaagaagg tttcttacca
     5641 tttatccatt ctaaaaattg ctgaaagtgc aaatcgatag atagagacct aggcttataa
     5701 actatttttt ttttcgaaga aaactgaaaa ataatcacac tccttccatt attatgagaa
     5761 tctcctgatt gtaaagttaa agaggttaat atatcatcat ctgataaatg gaatatatgt
     5821 acaagttcac ttctatcctt taaaaaacga taaattgatt ctacaatact atcaatttta
     5881 tttaaaataa attcaattaa aattcttact aaaacagggt attttttaat gagttcaaac
     5941 tttttgtctg gagatgataa tatttgatcc acataaaaat tatatctctc cttcggcgta
     6001 tccccaatta gattaccttt ctctctttct tcatttaaat cttgaataag ggttctaata
     6061 cttacataaa ccaaattttc atgaataaat ttcataatat catttttaat actagttaag
     6121 ttacatatcc tagataaatc atcataatta ttaacttgac tttttagact agaatccaaa
     6181 tatcttgatg ctatttctaa aaattcttta aaaaaagaag aaaaaggatt tgataaattt
     6241 tcttcgtttg aatacaattg atctacttct atattatttt tacaaacatt taaatattgg
     6301 taaaataaat ctatccattt attagttttt ttttgaaaaa gtaattcatt tattggaaca
     6361 ttattttcta tctccttttt cttttcagag ggatataata atttatcttt aattatttcc
     6421 atgaggttat tttctactat ttcgttcatt tattaccaac cctccttaaa aaaatcaact
     6481 aaccaaccca aagagttact tcaactcttt ggattggcca aaaatagcaa ttgctacatc
     6541 ggacatttta ctttgaaact aggacaaaat cttaagctcc aattgcatat tcttaagcta
     6601 acgcaaacaa tcgtactttg agggctaacc tctgtgtaac cgcctgctaa tttctttaat
     6661 tcttcttcag aaattttgct tacaaaggtt tgttcttgtt tttccatttg tatccctccc
     6721 ttcatttcaa attatattcc tacttttctt cttcttcacc accaacatgg tgtcggaacc
     6781 ccgccatttt ttggcggagt accgacatga aatggcgtag tcctaaataa aaaagagaaa
     6841 acgccctcac cgatccgatg aaggcgttaa caacatatga agctctggaa taagccctaa
     6901 ctgtctggct ttttctactg cttctatcct cgaagacaca tgtaacttgg tgaagattcg
     6961 cgatagatgt ctttctacac ttcgttggct tatatagtgt tttttagcaa tatctttatt
     7021 gcttaaaccg ttggcgacct cgaataatat ttcttgctca agctggctca gcgcaacatg
     7081 ttcttcattt tctaccatcc tagctttgac ttcacaaagc tgcaactggc gcagtaaatg
     7141 cacaggaata accgcttcat ctcttaacgc acagcgaatc gctgtaatca attgctcgct
     7201 tgttgccgtt ttactgacaa agcctgtaat gcctgattca atcaaataat taaaaaaagg
     7261 agcaacatca tatccggtat atatgacaat tttcgcattc ggttgttttt ggagaattcg
     7321 cttacttaat tcggtgccgc tcatatccgg catacgccaa tcaagcaaaa acacatcata
     7381 tgtacgttgt tccatcatct gttccgcttc tgtaccagat gtaacaagtt ctacttgaaa
     7441 atcttgttcg ctctcgagca tattctttgt cccttcacca acaagacgat gatcatcgac
     7501 aaccaaaatg tgaatcatct agtcattttt ctccttacta tgcttataat ggaatggtga
     7561 tccgtatatc tagcccctcg ccaagttgtg acgtcatctc gcatgttcca cctaagcctt
     7621 gcacgcgctc ttttatgcca aacaaaccaa gcttgccatc acaatgattt aatgtttcca
     7681 tgtctatacc tataccattg tcaacgtaat gaagttgaac ctcctcattt tgagcctcca
     7741 atgttagttg aatatacgat gcctgcgaat gtttcagcgc attgtttaat aattcttgga
     7801 taatccgata aatagcaagc tcccgttctt ccttcaattt agctgataaa tgaacattca
     7861 attctacttt tatattcaca cgtaattgga atcgttggat taactgtaca atcgcttcct
     7921 ttaatccgat ttgactaaaa aatggcggtc ttaattcatg gcatatctcc cttgttaaat
     7981 caatcatatc tacgatttct tcttcaatgt agtttagctg ctttttcatt tcttccatac
     8041 gcatattttg aaagcgcaag ctctccactt tccgttttaa aacaattaaa tcttgaagaa
     8101 tcgtatcgtg caagtcaatg gctaactttc ttctttcctt ttccgaccat tgaaagatca
     8161 aacgagtgag ccaaacagaa ttgcttcttt cctttaagct gtctaactct tttatgaaat
     8221 cctctacttt taacatattt tcaatcgtga cgctggcata atagacaatg gtggataacc
     8281 aatctttttg ttctgcccga aatggaatgg gatgtttctt ttttcctatt aatagtaaag
     8341 gactttggcg gaacgagcct acaaagaccg caaacaaacg cttattttca atgatttttc
     8401 caatcgaaag tggttgacca tccatctttt tgattaactt catccactct ttttcatcgc
     8461 aatgaaaagg tgcttgcata tttttgacct ctaattgtac aaaagccaga ttctctatat
     8521 ctaatacttc tctcacttct ttttccatgc atcggatcac ttcttgcatg tttttctgtt
     8581 tttttaatgt ttcgccgatt cgatatatac tttcttggta aaagtctttt tgtacatata
     8641 acttagctct taatgttcga tcgaaacatt cttttaaata gagagcaagg atatttatta
     8701 tataaattag taaaaacaac aaagcgaatt caacaagtct ccacgcttca ttgacaaatt
     8761 tggcataaag caatgtaata agaaaagctg ggaggaatga taaaaaagca tagtacacaa
     8821 agcgattgat ggaaaactgc aatcgaaata accgatctgt acaaattaaa taaagaaaga
     8881 cagatggcaa cacaaaaagc gaagtaacgg ttaattcagc tggtagtacc cattttccaa
     8941 tgattagaac cgggagacta taaaataaaa caaatggtgc ggcagatagc ccaatggcta
     9001 aaagcaaacc tttgaccgta ttttgctgtt catctttttt aagataaacg aatccttttc
     9061 ctaacaaagc cagaatgatg accatgttga taaaaaatgt agccaatagc acttccgctt
     9121 cacttatcga acgaaacgat aaagatatga gtgaggcaac gaacatcact aagttaaacc
     9181 cataaaggga aaagacgatt tttttcgcga accacacttt cttttctttt tcaaaataat
     9241 gatgaacgaa gtgcaaaaag acggcaggca atattaaaaa tataccgtta aacatttgcc
     9301 ttccaaacag tgcatatttc gctgataagc tggcacttat ataacaaagc cctatcaata
     9361 ataaaaagta aataagcacg gtggccgatt tgtcatccgg ccgcagacga agcaaaaaaa
     9421 tgcttaaact aacagcgaat agaaaaaaac ccatcggcaa ggcgatataa tacagccatt
     9481 gttccgttcc atgcgattta tagtcaaccg tcaatgtttg gatttgattt tctctctgaa
     9541 tcgttattgt tttcgcttgt tcaattgttt tgtatttccg gacagtaaaa tgggagagcg
     9601 ggggctttcc gtccactttc agcacttgat cattaatatg aatgccattt ttttttgccc
     9661 aaccaaactc gtaaatatcg cttacaacaa tatcaccgtt cttatttttc tctacatcca
     9721 tgccgatcaa tggatgcttc acattgaaaa tggttaaata tagtgccaca atcgcagcta
     9781 cgcttatcac ggtaatcgtt cgtctcatcg ctctctactt ccaatatcca aggggggtaa
     9841 cggattggct aaataccttt attatcgctt gaacttcgag ttcatctaaa cctactaagc
     9901 ttactgtacc actttttaat ttttcaataa cctctggatt ttccattaaa aaacgaatca
     9961 tttcttgcat atttactccc tccgaaatta taaaattatc atttttagtg cggatttgta
    10021 cctatcgttt acatccagtt tctcgatttc ttgcaaagcc ttttccttgt aaatctggct
    10081 catcactttt gcatagtaaa acgctccttc ctgttccatt tgctgaatca ctttttcctt
    10141 ttggacataa agctcattgt aatcaacatg attgctaaaa tactgtttca gtaagttatc
    10201 ccgtttactt gttagcatgt acaaaatcgg aagcgacttc ttcttcgctt tgatgtcgtt
    10261 cttctcatcc caacgataca acgcatcgat atcattttcg atttgtgcag caattcctat
    10321 atcattggca tacttttcaa tgatgacagt gtttttttca gcgcctgcca atgcagttcc
    10381 gacgaaacac gccatggcta ctagagaacc cgattttttc ttcaccattt ctatatactc
    10441 cgcttccgtc tgtatgtcat atgtgacatc catatgctgc ccgtttacgc tttgaagcaa
    10501 tcgagatgaa atatataact gtgcctttgc ttttttatga tcatcaataa gtaaatccac
    10561 aatagctttt tgtcctgcta acaataaccc tatggctata tgaagtgaag ttgtaagagg
    10621 tgctttgttc cacgtggttt ccaagctgtc ttgatcaacg aggtcatcga gcatatctcc
    10681 cgccaacatc atcagttcta cagacgctgc gattcgttca gtttccttca tgttttcgga
    10741 aaacatctga taatgcacac cagctaagcg gccgaacaaa aagtcgcttt gttttgtgtg
    10801 ctcctctatt gatgtcaata ccgactcttt taaagacggt acggaaaaag atgctgtaac
    10861 gatctctttc atttgcatct tcactgcttc tttttcgatc atttcagcaa caccttgcat
//
